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Title:
HETEROGENEOUS POLYPEPTIDE EXPRESSION CASSETTE
Document Type and Number:
WIPO Patent Application WO/2015/166640
Kind Code:
A1
Abstract:
The present invention addresses the problem of providing: a signal peptide which enables the highly efficient secretion of a heterogeneous polypeptide produced in a cell of a bacterium belonging to the genus Bifidobacterium to the outside of the cell; an expression cassette which enables the efficient secretion of the heterogeneous polypeptide to the outside of the cell; an expression vector for the heterogeneous polypeptide; and a bacterium belonging to the genus Bifidobacterium, in which the heterogeneous polypeptide can be secreted. A means for solving the problem is a bacterium belonging to the genus Bifidobacterium, which is transformed with a vector having an expression cassette inserted thereinto, wherein the expression cassette comprises promoter DNA capable of acting in a bacterium belonging to the genus Bifidobacterium, DNA encoding the secretion signal peptide, DNA encoding a single-strand antibody having an anti-tumor activity, and terminator DNA capable of acting in a bacterium belonging to the genus Bifidobacterium in this order. The single-strand antibody produced in the Bifidobacterium bacterium can be secreted efficiently to the outside of a cell of the bacterium.

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Inventors:
KOSEKI KOICHI (JP)
MASAKI TAKESHI (JP)
SHIOYA KOICHIRO (JP)
SHIMIZU HITOMI (JP)
TSUKAMOTO MASAO (JP)
KOBAYASHI SATOSHI (JP)
MATSUMURA TOMIO (JP)
SHIMATANI YUKO (JP)
Application Number:
PCT/JP2015/002132
Publication Date:
November 05, 2015
Filing Date:
April 17, 2015
Export Citation:
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Assignee:
ANAEROPHARMA SCIENCE INC (JP)
International Classes:
A61K35/74; C12N15/09; A61K39/395; A61P35/00; A61P37/04; C12N1/21; C07K16/18; C07K16/28
Domestic Patent References:
WO2011093465A12011-08-04
WO2011093468A12011-08-04
WO2014010758A12014-01-16
Foreign References:
JP2004531245A2004-10-14
Other References:
DATABASE GenBank [O] 4 May 2012 (2012-05-04), SHKOPOROV,A.N: "NlpC/P60 family protein [Bifidobacterium longum subsp. longum 35B]", XP055235291, retrieved from ncbi Database accession no. EIJ27286
DATABASE GenBank [O] 4 May 2012 (2012-05-04), SHKOPOROV,A.N.: "hypothetical protein HMPREF1313_0372 [Bifidobacterium longum subsp. longum 1-6B", XP055235294, retrieved from ncbi Database accession no. EIJ22210
DATABASE GenBank [O] 31 January 2014 (2014-01-31), HAO,Y.: "Hypothetical protein BBMN68_1126 [Bifidobacterium longum subsp. longum BBMN68", XP055235297, retrieved from ncbi Database accession no. ADQ02103
DATABASE genbank [O] 4 May 2012 (2012-05-04), SHKOPOROV,A.N: "G5 domain protein [Bifidobacterium longum subsp. longum 35B]", XP055235306, retrieved from ncbi Database accession no. EIJ27376
DATABASE GenBank [O] 29 January 2011 (2011-01-29), FUKUDA,S.: "putative cell surface protein [Bifidobacterium longum subsp. infantis 157F]", XP055235299, retrieved from ncbi Database accession no. BAJ72090
DATABASE GenBank [O] 17 June 2013 (2013-06-17), OBERG,T.S: "Autotransporter adhesin [Bifidobacterium longum D2957", XP055235303, retrieved from ncbi Database accession no. EPE38071
JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE, vol. 244, no. 9, 2013, pages 809 - 815, XP008184468
Attorney, Agent or Firm:
HIROTA, Masanori (JP)
Masaki Hirota (JP)
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