Title:
HIGH-PURITY HUMAN CORTICOTROPIN OR ANALOG THEREOF, AND LARGE-SCALE PREPARATION METHOD THEREFOR
Document Type and Number:
WIPO Patent Application WO/2022/217395
Kind Code:
A1
Abstract:
The present invention belongs to the technical field of preparation methods for polypeptides, and particularly relates to high-purity corticotropin (human-derived sequence) or an analog thereof, and a large-scale preparation method therefor, which method comprises the main steps of: firstly, coupling amino acids in sequence from a C terminus to an N terminus by means of an Fmoc solid-phase synthesis method to acquire a crude peptide-resin of corticotropin (human-derived sequence) or an analog thereof with a protective group, wherein the reaction temperature of C-15 peptide synthesis is 40-60ºC; then cutting and settling same to obtain a crude product of the corticotropin (human-derived sequence) or the analog thereof; and then separating and purifying same by means of liquid chromatography to acquire a high-purity finished product. The chromatographic purity of a sample of the corticotropin (human-derived sequence) or the analog thereof prepared by the present invention reaches 99% or above, the stability is good, and the yield of a target peptide is ≥63%.
Inventors:
MENG JUNDONG (CN)
RUI KANGNING (CN)
LIU BIN (CN)
HAN YUANYUAN (CN)
CHEN SONG (CN)
ZHANG HAONING (CN)
RUI KANGNING (CN)
LIU BIN (CN)
HAN YUANYUAN (CN)
CHEN SONG (CN)
ZHANG HAONING (CN)
Application Number:
PCT/CN2021/086395
Publication Date:
October 20, 2022
Filing Date:
April 12, 2021
Export Citation:
Assignee:
NANJING HANXIN PHARMACEUTICAL TECH CO LTD (CN)
International Classes:
C07K14/695; A61K38/35; A61P21/02; A61P29/00; A61P37/08; C07K1/06
Domestic Patent References:
| WO2013098802A2 | 2013-07-04 |
Foreign References:
| US4055524A | 1977-10-25 | |||
| GB1586974A | 1981-03-25 | |||
| CN102675453A | 2012-09-19 |
Other References:
OELOFSEN, W.: "The chemistry of the adrenocorticotropins and the melanotropins", PHARMACOLOGY & THERAPEUTICS, vol. 1, no. 3, 1 January 1975 (1975-01-01), GB , pages 459 - 500, XP023858293, ISSN: 0163-7258
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