Title:
HIGH-RESOLUTION SPATIAL TRANSCRIPTOME
Document Type and Number:
WIPO Patent Application WO/2022/192721
Kind Code:
A3
Abstract:
Example spatial transcriptomics techniques use "continuous" polony arrays on a customized gel surface for spatial barcoding. By screening polyacrylamide (PAA) gel fabrication conditions, polonies formed on a crosslinked PAA gel were shown to exhibit a continuous, homogenous DNA distribution, which is highly suited for tissue barcoding applications. Compared with widely used polonies formed in flow cells that utilize linear PAA gels, continuous polonies showed efficient DNA amplification and restriction digestion to generate capture oligo arrays, which have a significantly better spatial RNA capturing performance. In addition, the crosslinked PAA gel showed sufficient constraints on lateral RNA diffusion and provides better mechanical strength and stability for tissue mapping assays than a semifluidic linear PAA gel used by previous methods.
Inventors:
GU LIANGCAI (US)
FU XIAONAN (US)
LIN SHIN (US)
SUN LI (US)
FU XIAONAN (US)
LIN SHIN (US)
SUN LI (US)
Application Number:
PCT/US2022/020028
Publication Date:
October 20, 2022
Filing Date:
March 11, 2022
Export Citation:
Assignee:
UNIV WASHINGTON (US)
International Classes:
C12Q1/6874; C08F20/56; C08F220/56; C08F222/38; C12Q1/6813; C12Q1/6869
Domestic Patent References:
| WO2019213254A1 | 2019-11-07 | |||
| WO2020076976A1 | 2020-04-16 |
Foreign References:
| US20070087362A1 | 2007-04-19 | |||
| US20200002764A1 | 2020-01-02 | |||
| US20160256846A1 | 2016-09-08 | |||
| US20160253584A1 | 2016-09-01 |
Other References:
FU XIAONAN, SUN LI, CHEN JANE Y., DONG RUNZE, LIN YIING, PALMITER RICHARD D., LIN SHIN, GU LIANGCAI: "Continuous Polony Gels for Tissue Mapping with High Resolution and RNA Capture Efficiency", BIORXIV, 17 March 2021 (2021-03-17), XP055983312, Retrieved from the Internet [retrieved on 20221121], DOI: 10.1101/2021.03.17.435795
Attorney, Agent or Firm:
MEAD, Katherine M. et al. (US)
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