Title:
HIGH SENSITIVE DETECTION METHOD FOR TARGET MOLECULE EXPLOITING SPECIFIC BINDING AND KIT FOR THE METHOD
Document Type and Number:
WIPO Patent Application WO/2007/010998
Kind Code:
A1
Abstract:
Disclosed is a label which is highly sensitive, can provide a narrow distribution in values of measurements, can be determined stably and is excellent in handling property. Also disclosed is a labeling method using the label. Further disclosed is an analysis method. A determination method using an oligonucleotide as a novel label; a labeled chemical substance; and an oligonucleotide for use as a label; and a kit for the determination. The determination method may be a method for cleaving a nucleic acid with a nuclease using an oligonucleotide bound to a chemical substance as a label. The method may be a method for cleaving a nucleic acid with a flap endonuclease. The method may be a method for detection, identification or quantification by invader method. The method may be a method for detection, identification or quantification of a target molecule in a sample, comprising the steps of contacting a chemical substance bound to an oligonucleotide as a label (referred to as "a labeled chemical substance", hereinbelow) with a sample which contains the target molecule or which is suspected to contain the target molecule to form a complex of the labeled chemical substance and the target molecule and then determining the oligonucleotide in the complex by invader method.
Inventors:
XIE MINJUE (JP)
FUKUI KOICHI (JP)
HORIE MASANORI (JP)
KAGEYAMA YOSHITAKA (JP)
MATSUMOTO KAZUKO (JP)
FUKUI KOICHI (JP)
HORIE MASANORI (JP)
KAGEYAMA YOSHITAKA (JP)
MATSUMOTO KAZUKO (JP)
Application Number:
PCT/JP2006/314419
Publication Date:
January 25, 2007
Filing Date:
July 20, 2006
Export Citation:
Assignee:
JAPAN SCIENCE & TECH AGENCY (JP)
XIE MINJUE (JP)
FUKUI KOICHI (JP)
HORIE MASANORI (JP)
KAGEYAMA YOSHITAKA (JP)
MATSUMOTO KAZUKO (JP)
XIE MINJUE (JP)
FUKUI KOICHI (JP)
HORIE MASANORI (JP)
KAGEYAMA YOSHITAKA (JP)
MATSUMOTO KAZUKO (JP)
International Classes:
C12N15/09; C12Q1/68; G01N33/532
Foreign References:
| JPH03231151A | 1991-10-15 | |||
| US20040214176A1 | 2004-10-28 |
Other References:
WATANABE N.: "Seitainai Biryo Busshitsu no Kokando Kenshutsuho no Kaihatsu to Rinsho Oyo", LAB. CLIN. PRACT., vol. 20, no. 2, 2002, pages 110 - 114, XP003007608
Attorney, Agent or Firm:
SAEKI, Norio (Aminosan Kaikan Building, 15-8, Nihonbashi 3-chom, Chuo-ku Tokyo 27, JP)
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