Title:
HIGH-SPECIFIC-ACTIVITY ACIDIC MANNANASE MUTANT
Document Type and Number:
WIPO Patent Application WO/2024/103615
Kind Code:
A1
Abstract:
Provided is a high-specific-activity acidic mannanase mutant. The mutant comprises at least one mutation site of F46S/Q, Y132F, T148K/R, F223L/W/M, L259M, S263E/T/D, W264F/Y/M/V, T266F/K/Y/L/G/Q, P335Q/E/K/T, and D336G. The specific activity of said mutant is significantly higher than that of a wild-type mannanase, and said mutant can be widely applied to the field of feed.
Inventors:
WU XIUXIU (CN)
LI XINPEI (CN)
CHEN GANG (CN)
LI XINPEI (CN)
CHEN GANG (CN)
Application Number:
PCT/CN2023/087239
Publication Date:
May 23, 2024
Filing Date:
April 10, 2023
Export Citation:
Assignee:
QINGDAO VLAND BIOTECH GROUP CO LTD (CN)
International Classes:
C12N9/24; A23K20/189; C12N9/42; C12N15/56
Domestic Patent References:
| WO2023041040A1 | 2023-03-23 |
Foreign References:
| CN115838706A | 2023-03-24 | |||
| CN115838707A | 2023-03-24 | |||
| CN111117987A | 2020-05-08 | |||
| CN107083374A | 2017-08-22 | |||
| CN103525790A | 2014-01-22 | |||
| CN110577946A | 2019-12-17 |
Other References:
SHANSHA LI, DEHAI LIU; WENTAO DIAO; XIAOFEI CHEN; SHUJING QUAN: "Screening of thermostable mutants of β-mannanase ManA from Aspergillus niger", CHINA BREWING, vol. 41, no. 3, 23 May 2022 (2022-05-23), pages 62 - 68, XP093170532, DOI: 10.11882/j.issn.0254-5071.2022.03.011
NATTAPOL ARUNRATTANAMOOK ET AL.: "Engineering of β-mannanase from Aspergillus niger to Increase Product Selectivity towards Medium Chain Length Mannooligosaccharides", JOURNAL OF BIOSCIENCE AND BIOENGINEERING, vol. 130, no. 5, 26 July 2020 (2020-07-26), XP086301031, ISSN: 1347-4421, DOI: 10.1016/j.jbiosc.2020.07.001
NATTAPOL ARUNRATTANAMOOK ET AL.: "Engineering of β-mannanase from Aspergillus niger to Increase Product Selectivity towards Medium Chain Length Mannooligosaccharides", JOURNAL OF BIOSCIENCE AND BIOENGINEERING, vol. 130, no. 5, 26 July 2020 (2020-07-26), XP086301031, ISSN: 1347-4421, DOI: 10.1016/j.jbiosc.2020.07.001
Attorney, Agent or Firm:
UNITALEN ATTORNEYS AT LAW CO., LTD. (CN)
Download PDF: