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Title:
HIGH THROUGHPUT MUTAGENESIS SCREENING METHOD
Document Type and Number:
WIPO Patent Application WO2003076580
Kind Code:
A3
Abstract:
The present invention provides mutagenesis methods for protein engineering. In particular, the present inventionprovides mutagenesis methods that include feedback adjustment from systematic result evaluation. More specifically, the invention provides site-saturation mutagenesis methods that screen for variants with one or more desirable protein properties and evaluates screening results to provide feedback for repeat screening and construction of new libraries. The invention further provides cutinase variants that provide improved stability and activity compared with the wild type cutinase.

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Inventors:
BOTT RICHARD R (US)
KELLIS JAMES T JR (US)
MORRISON THOMAS B (US)
Application Number:
PCT/US2003/006908
Publication Date:
April 28, 2005
Filing Date:
March 05, 2003
Export Citation:
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Assignee:
GENENCOR INT (US)
BOTT RICHARD R (US)
KELLIS JAMES T JR (US)
MORRISON THOMAS B (US)
International Classes:
C12N9/18; (IPC1-7): C12N9/20
Foreign References:
US5352594A1994-10-04
Other References:
BOSTON M. ET AL.: "Structure and function of engineered Pseudomonas mendocina lipase", METHODS ENZYMOL., vol. 284, 1997, pages 298 - 317, XP008044067
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