HIGH-THROUGHPUT NEXT-GENERATION SEQUENCING METHOD FOR DOUBLE-STRANDED NUCLEOTIDE SEQUENCING

Title:

HIGH-THROUGHPUT NEXT-GENERATION SEQUENCING METHOD FOR DOUBLE-STRANDED NUCLEOTIDE SEQUENCING

Document Type and Number:

WIPO Patent Application WO/2020/043174

Kind Code:

A1

Abstract:

Provided is a high-throughput sequencing kit, comprising the following components: 1) a upstream sequencing adapter; 2) M row-encoding adapters; 3) N column-encoding adapters; and 4) a downstream sequencing adapter, where M and N are integers greater than or equal to 1. Also disclosed is a high-throughput sequencing method. The method can realize a quantitative analysis of high-throughput sequencing and has excellent application prospects.

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Inventors:

LI JIN (CN)
CHANG YONG (CN)
JIANG MINGXING (CN)
GAO YA (CN)
LI YOU (CN)
LIU JIAN (CN)
WAN JINQIAO (CN)

Application Number:

PCT/CN2019/103398

Publication Date:

March 05, 2020

Filing Date:

August 29, 2019

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Assignee:

HITGEN INC (CN)

International Classes:

C12Q1/6869; C12N15/11; C12Q1/6806; C12Q1/6809

Foreign References:

CN102061335A	2011-05-18
CN105602936A	2016-05-25
CN102978206A	2013-03-20
CN105154444A	2015-12-16

Other References:

CLARKE, L.J. ET AL.: "Modular Tagging of Amplicons Using A Single PCR for High-Throughput Sequencing", MOLECULAR ECOLOGY RESOURCES, vol. 14, 31 December 2014 (2014-12-31), pages 117 - 121, XP055580614, DOI: 20191114204510
汤道年等 (TANG, DAONIAN ET AL.): "四色荧光标记二硫键可逆终端的合成及在DNA合成测序中的应用 (Synthesis and Application of Four Fluorescence Labeled Nucleotides Through Disulfide as Reversible Terminators in DNA Sequencing by Synthesis)", 高等学校化学学报 (CHEMICAL JOURNAL OF CHINESE UNIVERSITIES), vol. 35, no. 11, 30 November 2014 (2014-11-30), pages 2346 - 2352, XP055299457, DOI: 20191114204920A

Attorney, Agent or Firm:

GAOYUNG INTELLECTUAL PROPERTY AGENCY (GENERAL PARTNERSHIP) (CN)

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