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Title:
HIGHLY EFFICIENT HYDROGEN PRODUCTION METHOD USING MICROORGANISM
Document Type and Number:
WIPO Patent Application WO/2004/074495
Kind Code:
A1
Abstract:
It is intended to obtain aerobic microbial cells in an amount sufficient for a reaction of evolving hydrogen, impart a hydrogen-evolving function to an aerobic microorganism within a short time and provide an industrially advantageous method of producing hydrogen. The above objects can be established by providing a highly efficient microbial hydrogen production method characterized by comprising culturing a microorganism having a formate dehydrogenase gene and a hydrogenase gene under aerobic conditions, culturing the thus obtained microbial cells under anaerobic conditions in a liquid culture medium containing a formic acid compound, and then using the thus obtained cells for evolving hydrogen.

Inventors:
YUKAWA HIDEAKI (JP)
YAMAMOTO NORIYUKI (JP)
Application Number:
PCT/JP2004/002092
Publication Date:
September 02, 2004
Filing Date:
February 23, 2004
Export Citation:
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Assignee:
RES INST INNOVATIVE TECH EARTH (JP)
SHARP KK (JP)
YUKAWA HIDEAKI (JP)
YAMAMOTO NORIYUKI (JP)
International Classes:
C12N1/15; C12N1/19; C12N1/21; C12P3/00; (IPC1-7): C12P3/00; C12N1/00; C12N1/15; C12N1/19; C12N1/21
Foreign References:
JPS61205492A1986-09-11
US6395521B12002-05-28
JPH04169178A1992-06-17
JP2002270209A2002-09-20
JPS5860992A1983-04-11
Other References:
LARSSON G. ET AL: "Kinetics of Escherichia coli hydrogen production during short term repeated aerobic-anaerobic fluctuations", BIOPROCESS ENGINEERING, vol. 9, no. 4, 1993, pages 167 - 172, XP002980304
TANISHO S. ET AL: "Fermentative hydrogen evolution by enerobacter aerogenes strain E.82005", INT'L J. OF HYDROGEN ENERGY, vol. 12, no. 9, 1987, pages 623 - 627, XP002980305
TANISHO S. ET AL: "Microbial fuel cell using Enterobacter aerogenes", J. OF ELECTROANALYTICAL CHEMISTRY, vol. 275, 1989, pages 25 - 32, XP002980306
Attorney, Agent or Firm:
Iwatani, Ryo (1-31 Dojima 2-chome, Kita-k, Osaka-shi Osaka, JP)
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