Title:
HIGHLY ORTHOGONAL UNIVERSAL SEQUENCES FOR USE IN NUCLEIC ACID ASSAYS
Document Type and Number:
WIPO Patent Application WO2006083925
Kind Code:
A3
Abstract:
The invention provides a set of highly orthogonal six-code universal sequences for use in bDNA singleplex and multiplex nucleic acid hybridization assays. The six-code orthogonal sequences do not cross-hybridize and thus, minimize or eliminate the 3-mer cross-hybridization inherent in the second and third generation bDNA assays. The highly orthogonal universal sequences may be used in singleplex or multiplex bDNA assays quantitatively and qualitatively to determine mRNA levels in a sample; to screen for and genotype targets, such as viruses, that are present in low volumes in a sample; to screen for and genotype SNPs; and to measure changes in the amount of a gene in a sample such as when gene amplifications or deletions occur. The highly orthogonal universal sequences may also be used as universal capture probes to selectively bind assay components in a way that facilitates their further analysis.
Inventors:
ZHENG MINXUE (US)
AHLE DAVID (US)
WARNER BRIAN (US)
WU MING (US)
CHANG CHU-AN (US)
AHLE DAVID (US)
WARNER BRIAN (US)
WU MING (US)
CHANG CHU-AN (US)
Application Number:
PCT/US2006/003490
Publication Date:
November 23, 2006
Filing Date:
January 31, 2006
Export Citation:
Assignee:
BAYER HEALTHCARE LLC (US)
ZHENG MINXUE (US)
AHLE DAVID (US)
WARNER BRIAN (US)
WU MING (US)
CHANG CHU-AN (US)
ZHENG MINXUE (US)
AHLE DAVID (US)
WARNER BRIAN (US)
WU MING (US)
CHANG CHU-AN (US)
International Classes:
C12Q1/68
Domestic Patent References:
| WO1996006950A1 | 1996-03-07 |
Other References:
COLLINS M L ET AL: "A branched DNA signal amplification assay for quantification of nucleic acid targets below 100 molecules/ml", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 25, no. 15, 1997, pages 2979 - 2984, XP002208495, ISSN: 0305-1048
IANNONE M A ET AL: "MULTIPLEXED SINGLE NUCLEOTIDE POLYMORPHISM GENOTYPING BY OLIGONUCLEOTIDE LIGATION AND FLOW CYTOMETRY", CYTOMETRY, ALAN R. LISS, INC, vol. 39, no. 2, 2000, pages 131 - 140, XP001073442, ISSN: 0196-4763
SHEN L-P ET AL: "Quantification of cytokine mRNA in peripheral blood mononuclear cells using branched DNA (bDNA) technology", JOURNAL OF IMMUNOLOGICAL METHODS, ELSEVIER SCIENCE PUBLISHERS B.V.,AMSTERDAM, NL, vol. 215, no. 1-2, 1 June 1998 (1998-06-01), pages 123 - 134, XP004146499, ISSN: 0022-1759
IANNONE M A ET AL: "MULTIPLEXED SINGLE NUCLEOTIDE POLYMORPHISM GENOTYPING BY OLIGONUCLEOTIDE LIGATION AND FLOW CYTOMETRY", CYTOMETRY, ALAN R. LISS, INC, vol. 39, no. 2, 2000, pages 131 - 140, XP001073442, ISSN: 0196-4763
SHEN L-P ET AL: "Quantification of cytokine mRNA in peripheral blood mononuclear cells using branched DNA (bDNA) technology", JOURNAL OF IMMUNOLOGICAL METHODS, ELSEVIER SCIENCE PUBLISHERS B.V.,AMSTERDAM, NL, vol. 215, no. 1-2, 1 June 1998 (1998-06-01), pages 123 - 134, XP004146499, ISSN: 0022-1759
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