Title:
HIV PSEUDOVIRUS PARTICLE AND METHOD FOR PREPARATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/123375
Kind Code:
A1
Abstract:
Provided are a human immunodeficiency virus (HIV) pseudovirus particle and a method for preparation thereof. The pseudovirus particle comprises a conserved sequence fragment of Gag, Pol, and LTR regions on an HIV-1 gene as a target fragment and a bacteriophage MS2 protein. The method comprises: designing and selecting a target fragment and a bacteriophage MS2 gene sequence; directly synthesizing the two respectively; inserting the bacteriophage MS2 gene sequence into a pETDuet-1 carrier to obtain a pETDuet-MS2 carrier; inserting the target fragment into a pETDuet-MS2 vector to obtain a pETDuet-MS2-HIV recombinant expression vector. The phage MS2 gene sequence and the target fragment are respectively located between any enzymatic cleavage sites behind the tail ends of two T7 promoters of the pseudovirus particle.
Inventors:
JING HAIRONG (CN)
Application Number:
PCT/CN2021/143731
Publication Date:
July 06, 2023
Filing Date:
December 31, 2021
Export Citation:
Assignee:
BOE TECHNOLOGY GROUP CO LTD (CN)
CHENGDU BOE OPTOELECT TECH CO (CN)
CHENGDU BOE OPTOELECT TECH CO (CN)
International Classes:
C12N15/70; C12N7/04
Foreign References:
| CN106755043A | 2017-05-31 | |||
| CN103789277A | 2014-05-14 | |||
| CN1587420A | 2005-03-02 |
Other References:
LI, QING ET AL.: "Construction and Validation of a Novel HIV-1 Nucleic Acid Quantitative Internal Standard", CHINESE EXPERIMENTS AND CLINICAL INFECTIOUS DISEASES (ELECTRONIC EDITION), vol. 11, no. 1, 15 February 2017 (2017-02-15), XP009547447, ISSN: 1674-1358
DENG, JUNHUA ET AL.: "Research Progress on the Application of Pseudoviruses in the Detection of RNA Viruses", CHINA ANIMAL HEALTH INSPECTION, vol. 26, no. 11, 1 November 2009 (2009-11-01), XP009547458, ISSN: 1005-944X
GHOLAMI MOHAMMAD, RAVANSHAD MEHRDAD, BAESI KAZEM, SAMIEE SIAMAK M, NEGIN HOSSEINI ROZBAHANI, MOHRAZ MINOO: "Preparation and Evaluation of Ribonuclease-Resistant Viral HIV RNA Standards Based on Armored RNA Technology", IRANIAN BIOMEDICAL JOURNAL, PASTEUR INSTITUTE OF IRAN, IRAN, vol. 22, no. 6, 1 November 2018 (2018-11-01), Iran , pages 394 - 400, XP093075892, ISSN: 1028-852X, DOI: 10.29252/ibj.22.6.394
DENG, JUNHUA ET AL.: "Research Progress on the Application of Pseudoviruses in the Detection of RNA Viruses", CHINA ANIMAL HEALTH INSPECTION, vol. 26, no. 11, 1 November 2009 (2009-11-01), XP009547458, ISSN: 1005-944X
GHOLAMI MOHAMMAD, RAVANSHAD MEHRDAD, BAESI KAZEM, SAMIEE SIAMAK M, NEGIN HOSSEINI ROZBAHANI, MOHRAZ MINOO: "Preparation and Evaluation of Ribonuclease-Resistant Viral HIV RNA Standards Based on Armored RNA Technology", IRANIAN BIOMEDICAL JOURNAL, PASTEUR INSTITUTE OF IRAN, IRAN, vol. 22, no. 6, 1 November 2018 (2018-11-01), Iran , pages 394 - 400, XP093075892, ISSN: 1028-852X, DOI: 10.29252/ibj.22.6.394
Attorney, Agent or Firm:
AFD CHINA INTELLECTUAL PROPERTY LAW OFFICE (CN)
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