Title:
HPPD MUTANT PROTEIN HAVING HERBICIDE RESISTANCE AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2022/151352
Kind Code:
A1
Abstract:
Disclosed are a mutant HPPD protein and a nucleic acid or gene encoding the protein or a fragment. The HPPD protein or a biologically active fragment thereof retains or enhances the property of catalyzing conversion of a 4-hydroxyphenylpyruvic acid (4-HPPA) to a homogentisic acid, and is significantly less sensitive to an HPPD inhibitor than a wild-type HPPD. The present invention further relates to a method for obtaining a plant comprising expression vectors and host cells and having reduced sensitivity to HPPD inhibitor herbicides. The mutant protein having a single point mutation or multiple point mutations obtained in the present invention can tolerate four HPPD inhibitor herbicides such as mesotrione, topramezone, isoxaflutole, and tembotrione in Escherichia coli. The transgenic rice having a single point mutation or multiple point mutations in the present invention can tolerate four HPPD inhibitor herbicides such as mesotrione, topramezone, isoxaflutole, and tembotrione.
Inventors:
ZHANG BAOLONG (CN)
YANG YUWEN (CN)
ZHOU ZHENZHEN (CN)
LIU TINGLI (CN)
GUO DONGSHU (CN)
YANG YUWEN (CN)
ZHOU ZHENZHEN (CN)
LIU TINGLI (CN)
GUO DONGSHU (CN)
Application Number:
PCT/CN2021/072156
Publication Date:
July 21, 2022
Filing Date:
January 15, 2021
Export Citation:
Assignee:
JIANGSU ACAD AGRICULTURAL SCI (CN)
JIANGSU FLAG CROP PROTECTION TECH CO LTD (CN)
JIANGSU FLAG CROP PROTECTION TECH CO LTD (CN)
International Classes:
C12N9/02; A01H5/00; C12N15/53; C12N15/82
Domestic Patent References:
| WO2020221312A1 | 2020-11-05 |
Foreign References:
| CN110616203A | 2019-12-27 | |||
| EP2669373A1 | 2013-12-04 | |||
| CN108866092A | 2018-11-23 | |||
| CN103237894A | 2013-08-07 | |||
| CN102971428A | 2013-03-13 |
Attorney, Agent or Firm:
NANJING SUGAO PATENT AND TRADEMARK FIRM ( ORDINARY PARTNERSHIP ) (CN)
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