Title:
HUMAN INTESTINAL EPITHELIUM MODEL AND METHOD FOR PREPARING SAME
Document Type and Number:
WIPO Patent Application WO/2022/098052
Kind Code:
A1
Abstract:
The present invention relates to a method for preparing a human intestinal epithelial model. The human intestinal epithelial model, prepared by the method according to the present invention, has all characteristics of goblet cells, enteroendocrine cells, and Paneth cells, and thus can highly mimic the function of actual human intestinal cells, so that the human intestinal epithelial model can be effectively used for development of new drugs, evaluation of drug absorption and toxicity, or evaluation of adhesion of intestinal microorganisms, or as a composition for in vivo transplantation.
Inventors:
SON MI-YOUNG (KR)
KWON OHMAN (KR)
JUNG KWANG BO (KR)
LEE KYEONG-RYOON (KR)
KIM JANGHWAN (KR)
JUNG CHO-ROK (KR)
KWON OHMAN (KR)
JUNG KWANG BO (KR)
LEE KYEONG-RYOON (KR)
KIM JANGHWAN (KR)
JUNG CHO-ROK (KR)
Application Number:
PCT/KR2021/015694
Publication Date:
May 12, 2022
Filing Date:
November 02, 2021
Export Citation:
Assignee:
KOREA RES INST BIOSCIENCE & BIOTECHNOLOGY (KR)
International Classes:
C12N5/071
Foreign References:
| JP6296399B2 | 2018-03-20 | |||
| US20180320144A1 | 2018-11-08 | |||
| JP2019527068A | 2019-09-26 |
Other References:
SOICHIRO OGAKI, NOBUAKI SHIRAKI, KAZUHIKO KUME, SHOEN KUME: "Wnt and Notch Signals Guide Embryonic Stem Cell Differentiation into the Intestinal Lineages", STEM CELLS, ALPHAMED PRESS, vol. 31, no. 6, 22 June 2013 (2013-06-22), pages 1086 - 1096, XP055177743, ISSN: 10665099, DOI: 10.1002/stem.1344
RYAN FORSTER, KUNITOSHI CHIBA, LORIAN SCHAEFFER, SAMUEL G. REGALADO, CHRISTINE S. LAI, QING GAO, SAMIRA KIANI, HENNER F. FARIN, HA: "Human Intestinal Tissue with Adult Stem Cell Properties Derived from Pluripotent Stem Cells", STEM CELL REPORTS, CELL PRESS, UNITED STATES, vol. 2, no. 6, 1 June 2014 (2014-06-01), United States , pages 838 - 852, XP055311225, ISSN: 2213-6711, DOI: 10.1016/j.stemcr.2014.05.001
NEGORO RYOSUKE, TAKAYAMA KAZUO, KAWAI KANAE, HARADA KAZUO, SAKURAI FUMINORI, HIRATA KAZUMASA, MIZUGUCHI HIROYUKI: "Efficient Generation of Small Intestinal Epithelial-like Cells from Human iPSCs for Drug Absorption and Metabolism Studies", STEM CELL REPORTS, CELL PRESS, UNITED STATES, vol. 11, no. 6, 1 December 2018 (2018-12-01), United States , pages 1539 - 1550, XP055928877, ISSN: 2213-6711, DOI: 10.1016/j.stemcr.2018.10.019
KWON OHMAN, JUNG KWANG BO, LEE KYEONG-RYOON, SEUL YE, SON, LEE HANA, KIM JONG-JIN, KIM KWANGHO, LEE SEOP, SONG YOO-KYUNG, JUNG JA: "The development of a functional human small intestinal epithelium model for drug absorption ", SCIENCE ADVANCES, vol. 7, no. 23, 2 June 2021 (2021-06-02), XP055928873, DOI: 10.1126/sciadv.abh1586
RYAN FORSTER, KUNITOSHI CHIBA, LORIAN SCHAEFFER, SAMUEL G. REGALADO, CHRISTINE S. LAI, QING GAO, SAMIRA KIANI, HENNER F. FARIN, HA: "Human Intestinal Tissue with Adult Stem Cell Properties Derived from Pluripotent Stem Cells", STEM CELL REPORTS, CELL PRESS, UNITED STATES, vol. 2, no. 6, 1 June 2014 (2014-06-01), United States , pages 838 - 852, XP055311225, ISSN: 2213-6711, DOI: 10.1016/j.stemcr.2014.05.001
NEGORO RYOSUKE, TAKAYAMA KAZUO, KAWAI KANAE, HARADA KAZUO, SAKURAI FUMINORI, HIRATA KAZUMASA, MIZUGUCHI HIROYUKI: "Efficient Generation of Small Intestinal Epithelial-like Cells from Human iPSCs for Drug Absorption and Metabolism Studies", STEM CELL REPORTS, CELL PRESS, UNITED STATES, vol. 11, no. 6, 1 December 2018 (2018-12-01), United States , pages 1539 - 1550, XP055928877, ISSN: 2213-6711, DOI: 10.1016/j.stemcr.2018.10.019
KWON OHMAN, JUNG KWANG BO, LEE KYEONG-RYOON, SEUL YE, SON, LEE HANA, KIM JONG-JIN, KIM KWANGHO, LEE SEOP, SONG YOO-KYUNG, JUNG JA: "The development of a functional human small intestinal epithelium model for drug absorption ", SCIENCE ADVANCES, vol. 7, no. 23, 2 June 2021 (2021-06-02), XP055928873, DOI: 10.1126/sciadv.abh1586
Attorney, Agent or Firm:
FIRSTLAW P.C. (KR)
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