Title:
HUMAN LACRIMAL GLAND STEM CELL, AND DIFFERENTIATION CULTURE METHOD THEREFOR AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/274043
Kind Code:
A1
Abstract:
A human lacrimal gland stem cell, and a differentiation culture method therefor and an application thereof. The preservation number of the human lacrimal gland stem cell is CCTCC NO: C202076. The cell is primarily isolated and cultured from human normal lacrimal gland tissue. No exogenous gene is introduced into the cell. The cell can be used for physiological research on human normal cells, pathogenesis research on lacrimal gland and lacrimal gland-related diseases comprising xerophthalmia and tear secretion, toxicological research of environmental pollutants on lacrimal gland, pharmacological research on drugs and the like. The cell also provides a basis for cell therapy of related diseases such as human lacrimal gland dysfunction.
Inventors:
YE LIN (CN)
WANG LING (CN)
HAN CHUNXIA (CN)
ZHANG YONGXIN (CN)
YIN GUOGAN (CN)
WANG YUAN (CN)
YANG MEINA (CN)
GUO HUI (CN)
ZHAN WENZHU (CN)
LIAO ZIFANG (CN)
WANG LING (CN)
HAN CHUNXIA (CN)
ZHANG YONGXIN (CN)
YIN GUOGAN (CN)
WANG YUAN (CN)
YANG MEINA (CN)
GUO HUI (CN)
ZHAN WENZHU (CN)
LIAO ZIFANG (CN)
Application Number:
PCT/CN2022/100948
Publication Date:
January 05, 2023
Filing Date:
June 24, 2022
Export Citation:
Assignee:
SHENZHEN EYE HOSPITAL (CN)
International Classes:
C12N5/071; C12R1/91
Domestic Patent References:
| WO2020165404A1 | 2020-08-20 |
Foreign References:
| CN113444679A | 2021-09-28 | |||
| CN109486766A | 2019-03-19 | |||
| US20140199275A1 | 2014-07-17 | |||
| CN111836884A | 2020-10-27 | |||
| US20180230425A1 | 2018-08-16 |
Other References:
ACKERMANN PHILIPP, HETZ SUSANN, DIECKOW JULIA, SCHICHT MARTIN, RICHTER ANJA, KRUSE CHARLI, SCHROEDER INSA S., JUNG MATTHIAS, PAULS: "Isolation and Investigation of Presumptive Murine Lacrimal Gland Stem Cells", INVESTIGATIVE OPTHALMOLOGY & VISUAL SCIENCE, ASSOCIATION FOR RESEARCH IN VISION AND OPHTHALMOLOGY, US, vol. 56, no. 8, 8 July 2015 (2015-07-08), US , pages 4350, XP093017965, ISSN: 1552-5783, DOI: 10.1167/iovs.15-16475
XIAO SA, ZHANG YAN: "Establishment of long-term serum-free culture for lacrimal gland stem cells aiming at lacrimal gland repair", STEM CELL RESEARCH & THERAPY, vol. 11, no. 1, 1 December 2020 (2020-12-01), XP093017966, DOI: 10.1186/s13287-019-1541-1
LU LILI, WANG YONG; LIU LIANGPING; ZENG MEIZHEN; ZHONG XINGWU: "Isolation and identification of Stem Cells from Rabbit Lacrimal Gland in Vitro", ZHONGSHAN DAXUE XUEBAO (YIXUE KEXUE BAN) / JOURNAL OF SUN YAT-SEN UNIVERSITY, CHINESE ELECTRONIC PERIODICAL SERVICES, CHINA, vol. 35, no. 3, 31 May 2014 (2014-05-31), CHINA , pages 438 - 444, XP093017968, ISSN: 1672-3554, DOI: 10.13471/j.cnki.j.sun.yat-sen.univ(med.sci).2014.0073
"Mouse cell culture : methods and protocols", vol. 1940, 21 February 2019, SPRINGER NEW YORK, New York, NY, ISBN: 978-1-4939-9085-6, article MAKARENKOVA HELEN P., MEECH ROBYN: "Isolation and Propagation of Lacrimal Gland Putative Epithelial Progenitor Cells", pages: 169 - 180, XP009542133, DOI: 10.1007/978-1-4939-9086-3_12
LIN HUI, LIU YING, YIU SAMUEL: "Three Dimensional Culture of Potential Epithelial Progenitor Cells in Human Lacrimal Gland", TRANSLATIONAL VISION SCIENCE & TECHNOLOGY, ASSOCIATION FOR RESEARCH IN VISION AND OPHTHALMOLOGY, US, vol. 8, no. 4, 30 August 2019 (2019-08-30), US , pages 32, XP093017970, ISSN: 2164-2591, DOI: 10.1167/tvst.8.4.32
XIAO SA, ZHANG YAN: "Establishment of long-term serum-free culture for lacrimal gland stem cells aiming at lacrimal gland repair", STEM CELL RESEARCH & THERAPY, vol. 11, no. 1, 1 December 2020 (2020-12-01), XP093017966, DOI: 10.1186/s13287-019-1541-1
LU LILI, WANG YONG; LIU LIANGPING; ZENG MEIZHEN; ZHONG XINGWU: "Isolation and identification of Stem Cells from Rabbit Lacrimal Gland in Vitro", ZHONGSHAN DAXUE XUEBAO (YIXUE KEXUE BAN) / JOURNAL OF SUN YAT-SEN UNIVERSITY, CHINESE ELECTRONIC PERIODICAL SERVICES, CHINA, vol. 35, no. 3, 31 May 2014 (2014-05-31), CHINA , pages 438 - 444, XP093017968, ISSN: 1672-3554, DOI: 10.13471/j.cnki.j.sun.yat-sen.univ(med.sci).2014.0073
"Mouse cell culture : methods and protocols", vol. 1940, 21 February 2019, SPRINGER NEW YORK, New York, NY, ISBN: 978-1-4939-9085-6, article MAKARENKOVA HELEN P., MEECH ROBYN: "Isolation and Propagation of Lacrimal Gland Putative Epithelial Progenitor Cells", pages: 169 - 180, XP009542133, DOI: 10.1007/978-1-4939-9086-3_12
LIN HUI, LIU YING, YIU SAMUEL: "Three Dimensional Culture of Potential Epithelial Progenitor Cells in Human Lacrimal Gland", TRANSLATIONAL VISION SCIENCE & TECHNOLOGY, ASSOCIATION FOR RESEARCH IN VISION AND OPHTHALMOLOGY, US, vol. 8, no. 4, 30 August 2019 (2019-08-30), US , pages 32, XP093017970, ISSN: 2164-2591, DOI: 10.1167/tvst.8.4.32
Attorney, Agent or Firm:
WUHAN HUAQIANG PATENT AGENCY (GENERAL PARTNERSHIP) (CN)
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