HYBRIDOMA CELL STRAIN C1 FOR SECRETING ANTI-PAROMOMYCIN MONOCLONAL ANTIBODY AND USE THEREOF

Title:

HYBRIDOMA CELL STRAIN C1 FOR SECRETING ANTI-PAROMOMYCIN MONOCLONAL ANTIBODY AND USE THEREOF

Document Type and Number:

WIPO Patent Application WO/2018/103630

Kind Code:

A1

Abstract:

Disclosed are a hybridoma cell strain C1 for secreting an anti-paromomycin monoclonal antibody and the use thereof, belonging to the technical field of immunochemistry. A murine monoclonal cell strain C1 has been prepared through the conventional cell fusion technologies and deposited in the China General Microbiological Culture Collection Centre, and the deposit number is CGMCC No. 12025. The monoclonal antibody secreted by the cell strain is determined by the indirect competitive enzyme-linked immunosorbent assay, and the IC50 to paromomycin and neomycin is 0.61 and 2.43 μg/L respectively. The range of the recovery rate of paromomycin and neomycin in animal foodstuff is 64.56-105.85% and 54.08-100.55%. The hybridoma cell strain C1 provides a raw material for the immunological detection of the paromomycin residue in animal foodstuff, and has a practical application value.

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Inventors:

XU LIGUANG (CN)
CHEN YANNI (CN)
XU CHUANLAI (CN)
KUANG HUA (CN)
LIU LIQIANG (CN)
SONG SHANSHAN (CN)
WU XIAOLING (CN)
ZHENG QIANKUN (CN)

Application Number:

PCT/CN2017/114598

Publication Date:

June 14, 2018

Filing Date:

December 05, 2017

Export Citation:

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Assignee:

DELISI GROUP CO LTD (CN)
UNIV JIANGNAN (CN)

International Classes:

C12N5/20; C07K16/44; G01N33/543; G01N33/577

Foreign References:

CN106520705A	2017-03-22
CN102585006A	2012-07-18
CN1996022A	2007-07-11
CN102608319A	2012-07-25
CN104569325A	2015-04-29
CN102778563A	2012-11-14

Other References:

ISANGA, J. ET AL.: "Development of a monoclonal antibody assay and a lateral flow strip test for the detection of paromomycin residues in food matrices", FOOD AND AGRICULTURAL IMMUNOLOGY, vol. 28, no. 3, 27 January 2017 (2017-01-27), pages 355 - 373, XP055606719

Attorney, Agent or Firm:

BEIJING CHINAGOUB INTELLECTUAL PROPERTY AGENT LTD. (CN)

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