Title:
HYDROGEL SUBSTRATE FOR CELL EVALUATION, AND CELL EVALUATION METHOD
Document Type and Number:
WIPO Patent Application WO/2014/126228
Kind Code:
A1
Abstract:
In order to more accurately evaluate the infiltration behavior of cancer cells under conditions approaching the environment in a living body than the prior art, a novel cell culture system, which is a three-dimensional environment and in which a plurality of types of cells co-exist, is constructed. For that purpose, a hydrogel substrate for cell evaluation, which is constituted by hydrogel and has a fiber- or sheet-like form, is used. The hydrogel substrate has a region (A) inside a cross section (S) in a plane perpendicular to a longitudinal axis, the periphery of which does not touch the periphery of the cross section (S) and which contains first cells. In addition, the region (A) is constituted by hydrogel with a low modulus of elasticity compared to a region (B) outside the region (A) in the cross section (S).
Inventors:
YAMADA MASUMI (JP)
SEKI MINORU (JP)
KITAGAWA YOICHI (JP)
SEKI MINORU (JP)
KITAGAWA YOICHI (JP)
Application Number:
PCT/JP2014/053578
Publication Date:
August 21, 2014
Filing Date:
February 17, 2014
Export Citation:
Assignee:
UNIV CHIBA NAT UNIV CORP (JP)
International Classes:
C12N5/071; C12N5/07; C12Q1/02
Domestic Patent References:
| WO2012009682A2 | 2012-01-19 |
Foreign References:
| JP2013009598A | 2013-01-17 | |||
| JP2010110272A | 2010-05-20 | |||
| JP2012172055A | 2012-09-10 |
Other References:
YOICHI KITAGAWA ET AL.: "Saibo no Seicho Hoko o Seigyo suru Danmen Ihosei Hydrogel Fiber no Sakusei", JAPANESE SOCIETY FOR BIOMATERIALS SYMPOSIUM 2012 YOKOSHU, 2012, pages 329
CULLEN D. KACY ET AL.: "Microtissue Engineered Constructs with Living Axons for Targeted Nervous System Reconstruction", TISSUE ENGINEERING; PART A, vol. 18, no. 21-22, 2012, pages 2280 - 2289
RAOF ABDUL NURAZHANI ET AL.: "Bioengineering embryonic stem cell microenvironments for exploring inhibitory effects on metastatic breast cancer cells", BIOMATERIALS, vol. 32, 2011, pages 4130 - 4139
GURSKI A. LISA ET AL.: "Hyaluronic acid-based hydrogels as 3D matrices for in vitro evaluation of chemotherapeutic drugs using poorly adherent prostate cancer cells", BIOMATERIALS, vol. 30, 2009, pages 6076 - 6085
KAWANO TAKAHIRO ET AL.: "Elasticity boundary conditions required for cell mechanotaxis on microelastically-patterned gels", BIOMATERIALS, vol. 32, 2011, pages 2725 - 2733
CULLEN D. KACY ET AL.: "Microtissue Engineered Constructs with Living Axons for Targeted Nervous System Reconstruction", TISSUE ENGINEERING; PART A, vol. 18, no. 21-22, 2012, pages 2280 - 2289
RAOF ABDUL NURAZHANI ET AL.: "Bioengineering embryonic stem cell microenvironments for exploring inhibitory effects on metastatic breast cancer cells", BIOMATERIALS, vol. 32, 2011, pages 4130 - 4139
GURSKI A. LISA ET AL.: "Hyaluronic acid-based hydrogels as 3D matrices for in vitro evaluation of chemotherapeutic drugs using poorly adherent prostate cancer cells", BIOMATERIALS, vol. 30, 2009, pages 6076 - 6085
KAWANO TAKAHIRO ET AL.: "Elasticity boundary conditions required for cell mechanotaxis on microelastically-patterned gels", BIOMATERIALS, vol. 32, 2011, pages 2725 - 2733
Attorney, Agent or Firm:
TAKAHASHI MASAYOSHI (JP)
Masayoshi Takahashi (JP)
Masayoshi Takahashi (JP)
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