Title:
A HYGIENE DETECTION DEVICE
Document Type and Number:
WIPO Patent Application WO/2021/040649
Kind Code:
A3
Abstract:
The present invention relates to a hygiene detection device (1) which can be used in the fields such as food, water, agriculture, medicine, biology and chemistry, and that enables determination toxic characteristics caused by the contamination of bacteria that has the ability to form indole by decomposing tryptophan amino acid. The tryptophanase enzyme produced by E. coli or such bacteria reacts with the tryptophan, and pyruvic acid, ammonia and indole is formed from the tryptophan by deamination. In the inventive hygiene detection device (1), an incubation environment of 37 C is provided for the bacteria, and the bacteria is left for incubation at 37 C for 4-8 hours. Upon the warning received after 4-8 hours, 0.5 ml of Kovak's reactive is dripped on the indole that is produced by a bacterium. The Kovak's reactive is a biochemical reactive consisting of isoamyl alcohol, paradimethylaminobenzaldehyde and hydrochloric acid. The indole produced by the bacteria reacts with the para¬ dimethylaminobenzaldehyde, and they form a maroon colored layer on the surface of the Eppendorf in which they are located. The optical reader (6) senses the frequency of this maroon color, and the presence of bacteria, if any, is shown to the user via the LCD screen (8).
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Inventors:
TUNÇER HÜSRA (TR)
ERYILDIZ ATAKAN (TR)
KOLUMAN AHMET (TR)
ERYILDIZ ATAKAN (TR)
KOLUMAN AHMET (TR)
Application Number:
PCT/TR2020/050737
Publication Date:
June 17, 2021
Filing Date:
August 21, 2020
Export Citation:
Assignee:
PAMUKKALE UNIV (TR)
International Classes:
C12Q1/04
Domestic Patent References:
| WO2008007206A2 | 2008-01-17 |
Foreign References:
| US20100062467A1 | 2010-03-11 | |||
| US3378346A | 1968-04-16 |
Attorney, Agent or Firm:
ISIKLI, Irfan Can (TR)
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