Title:
IDENTIFICATION OF NOVEL CELL SURFACE MARKERS FOR PANCREATIC PROGENITOR CELLS AND DEFINITE ENDODERMAL CELLS
Document Type and Number:
WIPO Patent Application WO/2012/070014
Kind Code:
A3
Abstract:
Provided are methods of identifying, isolating and qualifying pancreatic progenitor cells and definite endodermal cells. Also provided are an isolated population of pancreatic progenitor cells, comprising at least 75% of cells having a TROP-2+ and/or TROP-2+/GPR50+ expression pattern and an isolated population of definite endodermal cells, comprising at least 50% of cells having a SOX17+/SOX7+/GSC+/CER+/FOXA2+/CXCR4+/NANOG expression pattern. Also provided are nucleic acid constructs comprising a reporter protein under the transcriptional regulation of SOX17 regulatory sequence or of PDX1 regulatory sequence, and cells comprising same, and methods and kits using same.
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Inventors:
BENVENISTY NISSIM (IL)
ITSKOVITZ-ELDOR JOSEPH (IL)
FISHMAN BETTINA (IL)
SEGEV HANNA (IL)
KITSBERG DANNY (IL)
ITSKOVITZ-ELDOR JOSEPH (IL)
FISHMAN BETTINA (IL)
SEGEV HANNA (IL)
KITSBERG DANNY (IL)
Application Number:
PCT/IB2011/055283
Publication Date:
August 23, 2012
Filing Date:
November 24, 2011
Export Citation:
Assignee:
STEM CELL THERAPEUTICS LTD (IL)
BENVENISTY NISSIM (IL)
ITSKOVITZ-ELDOR JOSEPH (IL)
FISHMAN BETTINA (IL)
SEGEV HANNA (IL)
KITSBERG DANNY (IL)
BENVENISTY NISSIM (IL)
ITSKOVITZ-ELDOR JOSEPH (IL)
FISHMAN BETTINA (IL)
SEGEV HANNA (IL)
KITSBERG DANNY (IL)
International Classes:
A61K35/12; G01N33/48; G01N33/53; A61K35/39
Foreign References:
| US20070212732A1 | 2007-09-13 | |||
| US20100272695A1 | 2010-10-28 | |||
| US20100061964A1 | 2010-03-11 | |||
| US20090263896A1 | 2009-10-22 | |||
| US20070217999A1 | 2007-09-20 |
Other References:
FONG ET AL.: "High expression of TROP2 correlates with poor prognosis in pancreatic cancer", BR. J. CANCER, vol. 99, no. 9, 21 October 2008 (2008-10-21), pages 1290 - 1295
SPENCE ET AL.: "Sox17 regulates organ lineage segregation of ventral foregut progenitor cells", DEV. CELL., vol. 17, no. 1, July 2009 (2009-07-01), pages 62 - 74
DHAWAN ET AL.: "Formation and regeneration of the endocrine pancreas", CURR. OPIN. CELL. BIOL., vol. 19, no. 6, December 2007 (2007-12-01), pages 634 - 645, XP022382270, DOI: doi:10.1016/j.ceb.2007.09.015
GOLDSTEIN ET AL.: "Trop2 identifies a subpopulation of murine and human prostate basal cells with stem cell characteristics.", PROC. NATL. ACAD. SCI. USA, vol. 105, no. 52, 30 December 2008 (2008-12-30), pages 20882 - 20887
TADA ET AL.: "Characterization of mesendoderm: a diverging point of the definitive endoderm and mesoderm in embryonic stem cell differentiation culture", DEVELOPMENT, vol. 132, no. 19, October 2005 (2005-10-01), pages 4363 - 4374, XP008054294, DOI: doi:10.1242/dev.02005
SPENCE ET AL.: "Sox17 regulates organ lineage segregation of ventral foregut progenitor cells", DEV. CELL., vol. 17, no. 1, July 2009 (2009-07-01), pages 62 - 74
DHAWAN ET AL.: "Formation and regeneration of the endocrine pancreas", CURR. OPIN. CELL. BIOL., vol. 19, no. 6, December 2007 (2007-12-01), pages 634 - 645, XP022382270, DOI: doi:10.1016/j.ceb.2007.09.015
GOLDSTEIN ET AL.: "Trop2 identifies a subpopulation of murine and human prostate basal cells with stem cell characteristics.", PROC. NATL. ACAD. SCI. USA, vol. 105, no. 52, 30 December 2008 (2008-12-30), pages 20882 - 20887
TADA ET AL.: "Characterization of mesendoderm: a diverging point of the definitive endoderm and mesoderm in embryonic stem cell differentiation culture", DEVELOPMENT, vol. 132, no. 19, October 2005 (2005-10-01), pages 4363 - 4374, XP008054294, DOI: doi:10.1242/dev.02005
Attorney, Agent or Firm:
G.E EHRLICH (1995) LTD. et al. (Ramat Gan, IL)
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