Title:
IMMOBILIZED ENZYME AND USE THEREOF AND METHOD THEREFOR FOR PREPARING ETHYLHEXYLGLYCERIN
Document Type and Number:
WIPO Patent Application WO/2024/000620
Kind Code:
A1
Abstract:
Provided are a fusion protein and an immobilized enzyme comprising the fusion protein. The fusion protein comprises an epoxide hydrolase enzyme active moiety and a chitin protein binding moiety. By means of utilizing the specific affinity of chitin with fusion proteins, efficient immobilization of epoxide hydrolase is achieved, and, using ethylhexyl glycidyl ether as a substrate, ethylhexylglycerin is efficiently prepared and obtained by using the immobilized enzyme. Using the immobilization method has advantages such as low costs, high enzyme immobilization efficiency, minimal enzyme activity loss, and strong specificity, fundamentally solving problems such as non-recoverability of free enzymes, poor stability, and low repeatability of conventional whole-cell immobilization methods. Problems such as deep-colored downstream separation and purification and protein residue are solved.
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Inventors:
ZHANG SHASHA (CN)
SHI LUQIU (CN)
SU GUIZHEN (CN)
SHI LUQIU (CN)
SU GUIZHEN (CN)
Application Number:
PCT/CN2022/104609
Publication Date:
January 04, 2024
Filing Date:
September 02, 2022
Export Citation:
Assignee:
NANJING HUASHI NEW MAT CO LTD (CN)
NANJING SHENGDE INST OF BIOTECHNOLOGY CO LTD (CN)
NANJING SHENGDE INST OF BIOTECHNOLOGY CO LTD (CN)
International Classes:
C12N11/10; C12N1/21; C12N15/70; C12P7/18
Foreign References:
| CN112359033A | 2021-02-12 | |||
| CN111187792A | 2020-05-22 | |||
| CN111979206A | 2020-11-24 |
Other References:
WU, XIXI: "Study of a Thermophilic Bacteria Chitin-binding Domain and Its Application in Immobilized Enzyme", BASIC SCIENCES, CHINA MASTER’S THESES FULL-TEXT DATABASE, no. 6, 15 June 2012 (2012-06-15)
BARBOSA OVEIMAR, ORTIZ CLAUDIA, BERENGUER-MURCIA ÁNGEL, TORRES RODRIGO, RODRIGUES RAFAEL C., FERNANDEZ-LAFUENTE ROBERTO: "Strategies for the one-step immobilization–purification of enzymes as industrial biocatalysts", BIOTECHNOLOGY ADVANCES., ELSEVIER PUBLISHING, BARKING., GB, vol. 33, no. 5, 1 September 2015 (2015-09-01), GB , pages 435 - 456, XP093125057, ISSN: 0734-9750, DOI: 10.1016/j.biotechadv.2015.03.006
DATABASE Protein 28 January 2014 (2014-01-28), ANONYMOUS : "Epoxide hydrolase-like protein [Novosphingobium aromaticivorans DSM 12444]", XP093125058, retrieved from NCBI Database accession no. ABD26703.1
BARBOSA OVEIMAR, ORTIZ CLAUDIA, BERENGUER-MURCIA ÁNGEL, TORRES RODRIGO, RODRIGUES RAFAEL C., FERNANDEZ-LAFUENTE ROBERTO: "Strategies for the one-step immobilization–purification of enzymes as industrial biocatalysts", BIOTECHNOLOGY ADVANCES., ELSEVIER PUBLISHING, BARKING., GB, vol. 33, no. 5, 1 September 2015 (2015-09-01), GB , pages 435 - 456, XP093125057, ISSN: 0734-9750, DOI: 10.1016/j.biotechadv.2015.03.006
DATABASE Protein 28 January 2014 (2014-01-28), ANONYMOUS : "Epoxide hydrolase-like protein [Novosphingobium aromaticivorans DSM 12444]", XP093125058, retrieved from NCBI Database accession no. ABD26703.1
Attorney, Agent or Firm:
NANJING ZHONGQING KEZHI INTELLECTUAL PROPERTY AGENCY OFFICE (GENERAL PARTNERSHIP) (CN)
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