Title:
IMMUNE CELL-TARGETING AND RECOGNIZING POLYPEPTIDE AND APPLICATIONS THEREOF
Document Type and Number:
WIPO Patent Application WO/2021/012251
Kind Code:
A1
Abstract:
An immune cell-recognizing polypeptide. The polypeptide comprises the following amino acid sequence: (a) an amino acid sequence containing C-terminus fragment sequence AILEVLQS of human triokinase/FMN cyclase and a homologous sequence thereof; or, (b) an amino acid sequence having basic similarity to the amino acid sequence described in (a), the basic similarity referring to having 70% or more in sequence identity with the amino acid sequence described in (a). Also disclosed is a nucleic acid sequence encoding the polypeptide; a polypeptide probe targeting and recognizing immune cells, comprising the polypeptide, and having a reporting group; a reagent kit comprising the probe, and relevant applications of the polypeptide or probe.
Inventors:
WEI YUAN'AN (CN)
LIU XUESHU (CN)
LIU XUESHU (CN)
Application Number:
PCT/CN2019/097654
Publication Date:
January 28, 2021
Filing Date:
July 25, 2019
Export Citation:
Assignee:
KEYANGLE LIFE TECH CO LTD (CN)
International Classes:
C12N9/12; A61K47/64; A61K49/00; C12N15/11; C12N15/54
Domestic Patent References:
| WO2010009368A2 | 2010-01-21 |
Foreign References:
| CN110885805A | 2020-03-17 | |||
| CN109207451A | 2019-01-15 |
Other References:
DATABASE Protein 2 December 2020 (2020-12-02), ANONYMOUS: "Triokinase/FMN cyclase [Homo sapiens]", XP055775554, retrieved from NCBI Database accession no. Q3LXA3.2
RODRIGUES JOAQUIM RUI, COUTO ANA, CABEZAS ALICIA, PINTO ROSA MARÍA, RIBEIRO JOÃO MEIRELES, CANALES JOSÉ, COSTAS MARÍA JESÚS, CAMES: "Bifunctional homodimeric triokinase/FMN cyclase: contribution of protein domains to the activities of the human enzyme and molecular dynamics simulation of domain movements", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 289, no. 15, 11 April 2014 (2014-04-11), pages 10620 - 10636, XP055775561, ISSN: 0021-9258, DOI: 10.1074/jbc.M113.525626
ALICIA CABEZAS , MARIA JESUS COSTAS , ROSA MARIA PINTO , ANA COUTO , JOSE CARLOS CAMESELLE: "Identification of human and rat FAD-AMP lyase (cyclic FMN forming) as ATP-dependent dihydroxyacetone kinases", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 338, no. 4, 30 December 2005 (2005-12-30), pages 1682 - 1689, XP027218348, ISSN: 0006-291X, DOI: 10.1016/j.bbrc.2005.10.142
FEICI DIAO, SHU LI, YANG TIAN, MIN ZHANG, LIANG-GUO XU, YAN ZHANG, RUI-PENG WANG, DANYING CHEN, ZHONGHE ZHAI, BO ZHONG, PO TIEN, : "Negative regulation of MDA5- but not RIG-I-mediated innate antiviral signaling by the dihydroxyacetone kinase", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, vol. 104, no. 28, 10 July 2007 (2007-07-10), pages 11706 - 11711, XP055775571, ISSN: 0027-8424, DOI: 10.1073/pnas.0700544104
GEISSMANN ET AL.: "Development of monocytes, macrophages and dendritic cells", SCIENCE, vol. 327, no. 5966, 5 February 2010 (2010-02-05), pages 656 - 61
KRISTIN WESTPHALEN ET AL.: "Sessile alveolar macrophages modulate immunity through connexin 43-based epithelial communication", NATURE, vol. 506, no. 7489, 27 February 2014 (2014-02-27), pages 503 - 506
FEICI DIAO ET AL.: "Negative regulation of MDA5- but not RIG-I-mediated innate antiviral signaling by the dihydroxyacetone kinase", PROC NATL ACAD SCI USA, vol. 104, no. 28, 10 July 2007 (2007-07-10), pages 11706 - 11
B.ERNI ET AL.: "Small Substrate, Big Surprise: Fold, Function and Phylogeny of Dihydroxyacetone Kinases", CELL. MOL. LIFE SCI., vol. 63, 2006, pages 890 - 900, XP019419138, DOI: 10.1007/s00018-005-5518-0
A. CABEZAS ET AL.: "Identification of human and rat FAD-AMP lyase (cyclic FMN forming) as ATP-dependent dihydroxyacetone kinases", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 338, 2005, pages 1682 - 1689, XP027218348, DOI: 10.1016/j.bbrc.2005.10.142
"GenBank", Database accession no. DKFZP586B1621
HUANG ZHIPING ET AL.: "Application of polypeptide fluorescent probes in protein detection", CHINESE SCIENCE: CHEMISTRY, vol. 43, 2013, pages 1013 - 1021
See also references of EP 3792355A1
RODRIGUES JOAQUIM RUI, COUTO ANA, CABEZAS ALICIA, PINTO ROSA MARÍA, RIBEIRO JOÃO MEIRELES, CANALES JOSÉ, COSTAS MARÍA JESÚS, CAMES: "Bifunctional homodimeric triokinase/FMN cyclase: contribution of protein domains to the activities of the human enzyme and molecular dynamics simulation of domain movements", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 289, no. 15, 11 April 2014 (2014-04-11), pages 10620 - 10636, XP055775561, ISSN: 0021-9258, DOI: 10.1074/jbc.M113.525626
ALICIA CABEZAS , MARIA JESUS COSTAS , ROSA MARIA PINTO , ANA COUTO , JOSE CARLOS CAMESELLE: "Identification of human and rat FAD-AMP lyase (cyclic FMN forming) as ATP-dependent dihydroxyacetone kinases", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 338, no. 4, 30 December 2005 (2005-12-30), pages 1682 - 1689, XP027218348, ISSN: 0006-291X, DOI: 10.1016/j.bbrc.2005.10.142
FEICI DIAO, SHU LI, YANG TIAN, MIN ZHANG, LIANG-GUO XU, YAN ZHANG, RUI-PENG WANG, DANYING CHEN, ZHONGHE ZHAI, BO ZHONG, PO TIEN, : "Negative regulation of MDA5- but not RIG-I-mediated innate antiviral signaling by the dihydroxyacetone kinase", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, vol. 104, no. 28, 10 July 2007 (2007-07-10), pages 11706 - 11711, XP055775571, ISSN: 0027-8424, DOI: 10.1073/pnas.0700544104
GEISSMANN ET AL.: "Development of monocytes, macrophages and dendritic cells", SCIENCE, vol. 327, no. 5966, 5 February 2010 (2010-02-05), pages 656 - 61
KRISTIN WESTPHALEN ET AL.: "Sessile alveolar macrophages modulate immunity through connexin 43-based epithelial communication", NATURE, vol. 506, no. 7489, 27 February 2014 (2014-02-27), pages 503 - 506
FEICI DIAO ET AL.: "Negative regulation of MDA5- but not RIG-I-mediated innate antiviral signaling by the dihydroxyacetone kinase", PROC NATL ACAD SCI USA, vol. 104, no. 28, 10 July 2007 (2007-07-10), pages 11706 - 11
B.ERNI ET AL.: "Small Substrate, Big Surprise: Fold, Function and Phylogeny of Dihydroxyacetone Kinases", CELL. MOL. LIFE SCI., vol. 63, 2006, pages 890 - 900, XP019419138, DOI: 10.1007/s00018-005-5518-0
A. CABEZAS ET AL.: "Identification of human and rat FAD-AMP lyase (cyclic FMN forming) as ATP-dependent dihydroxyacetone kinases", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 338, 2005, pages 1682 - 1689, XP027218348, DOI: 10.1016/j.bbrc.2005.10.142
"GenBank", Database accession no. DKFZP586B1621
HUANG ZHIPING ET AL.: "Application of polypeptide fluorescent probes in protein detection", CHINESE SCIENCE: CHEMISTRY, vol. 43, 2013, pages 1013 - 1021
See also references of EP 3792355A1
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JIAQUAN IP LAW FIRM (CN)
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