Title:
IMMUNOGLOBULIN-BINDING MODIFIED PROTEIN
Document Type and Number:
WIPO Patent Application WO/2017/022672
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide an immunoglobulin-binding modified protein for the production of an affinity separation matrix having high antibody binding capacity. The present invention provides a protein that comprises two or more amino acid sequences derived from the E, D, A, B, or C domains of the protein A indicated in SED ID NOs. 1-5, in which the number of Lys included in an amino acid sequence derived from the domain furthest to the N-terminal side is more than the number of Lys included in amino acid sequences derived from other domains, and in which the number of Lys included from position 39 onward in an amino acid sequence derived from the domain furthest to the N-terminal side does not exceed the number of Lys included in positions 1-38.
Inventors:
NISHIHACHIJYO MASAKATSU (JP)
KONOIKE FUMINORI (JP)
NAKANO YOSHIYUKI (JP)
TAKANO MASAYUKI (JP)
YAMASHITA KEITA (JP)
KONOIKE FUMINORI (JP)
NAKANO YOSHIYUKI (JP)
TAKANO MASAYUKI (JP)
YAMASHITA KEITA (JP)
Application Number:
PCT/JP2016/072356
Publication Date:
February 09, 2017
Filing Date:
July 29, 2016
Export Citation:
Assignee:
KANEKA CORP (JP)
International Classes:
C07K14/31; B01D15/08; B01J20/24; C07K1/22; C12N1/15; C12N1/19; C12N1/21; C12N5/16; C12N15/09; C12P21/02; C12P21/08
Domestic Patent References:
| WO2012133349A1 | 2012-10-04 | |||
| WO2015034000A1 | 2015-03-12 |
Foreign References:
| JP2007252368A | 2007-10-04 | |||
| JP2013256484A | 2013-12-26 |
Other References:
DEIS, LN ET AL.: "Suppression of conformational heterogeneity at a protein-protein interface", PROC.NATL.ACAD.SCI.USA, vol. 112, no. 29, 2015, pages 9028, XP055362428
Attorney, Agent or Firm:
YASUTOMI & ASSOCIATES (JP)
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