Title:
IMMUNOGLOBULIN-BINDING POLYPEPTIDE
Document Type and Number:
WIPO Patent Application WO/2022/138718
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide a polypeptide, which has excellent alkali stability and allows the elution of immunoglobulin or a fragment of the same, which has been bound thereto, in a weakly acidic region, by modifying the amino acid sequence in an immunoglobulin-binding domain of Protein A. A polypeptide, which has a binding activity to immunoglobulin or a polypeptide having the Fc region of the same and which has excellent alkali stability and allows efficient elution of immunoglobulin or the polypeptide having the Fc region of the same, which has been bound thereto, under weakly acidic mild conditions, can be obtained by substituting serine at the 41st position in each immunoglobulin-binding domain of Protein A with an amino acid having a hydrophobic side chain, tyrosine or histidine.
Inventors:
MAJIMA EIJI (JP)
SHIMA ATSUSHI (JP)
SHIMA ATSUSHI (JP)
Application Number:
PCT/JP2021/047570
Publication Date:
June 30, 2022
Filing Date:
December 22, 2021
Export Citation:
Assignee:
PROTENOVA CO LTD (JP)
International Classes:
C12N15/31; C07K1/16; C07K14/31; C07K17/02; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N15/63; C12P21/02
Domestic Patent References:
| WO2012165544A1 | 2012-12-06 | |||
| WO2007097361A1 | 2007-08-30 | |||
| WO2000023580A1 | 2000-04-27 |
Foreign References:
| JP2016523959A | 2016-08-12 | |||
| JP2013256479A | 2013-12-26 | |||
| JP2010156687A | 2010-07-15 | |||
| CN112210013A | 2021-01-12 |
Other References:
PASTOR JOSE J., GRANADOS GIOVANNA, CARULLA NATÀLIA, RABANAL FRANCESC, GIRALT ERNEST: "Redesign of Protein Domains Using One-Bead-One-Compound Combinatorial Chemistry", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 129, no. 48, 1 December 2007 (2007-12-01), pages 14922 - 14932, XP055945845, ISSN: 0002-7863, DOI: 10.1021/ja073969x
TATIANA A. TATSUSOVATHOMAS L. MADDEN, FEMS MICROBIOL. LETT., vol. 174, 1999, pages 247 - 250
JANSON, J.-C.: "Protein purification", pages: 221 - 258
TATIANA A. TATSUSOVATHOMAS L. MADDEN, FEMS MICROBIOL. LETT., vol. 174, 1999, pages 247 - 250
JANSON, J.-C.: "Protein purification", pages: 221 - 258
Attorney, Agent or Firm:
TANAKA, Junya et al. (JP)
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