Background

The introduction of hybridoma technology has revolutionized the bio edical field by allowing for the production of unlimited quantities of antibodies of a desired specificity (hereinafter monoclonal antibodies) . In 1975, Kohler and Milstein (Nature 256:495 (1975)) disclosed that monoclonal antibodies could be produced by hybrid cell lines (hereinafter hybridomas) prepared by the fusion of spleen cells from an immunized animal with myeloma cells.

Monoclonal antibodies provide highly specific, well-characterized reagents. They have found wide applications <_in vitro for the identification, purification, and characterization of antigens.

When the antigenic determinants recognized by the monoclonal antibodies are cell surface tumor-as¬ sociated antigens, these reagents may also spe¬ cifically accumulate at the tumor site. In- creasingly they are being tested ij vivo for tumor- imaging and therapy (Levy and Miller, Ann. Rev. Med. 34:107 (1983)).

In some cases, treatment with monoclonal antibodies alone has resulted in partial or complete regression of tumors. See papers in the proceeding of the Wistar Symposium on Immunodiagnosis and Immunotherapy with C017-1A MAb in Gastrointestinal Cancer (Hybridoma 5:Suppl. 1 (1986)). Much effort has also been focused on modifications of monoclonal

antibodies for immunodiagnostic and i muno- therapeutic applications. For example, monoclonal antibodies can be used for targeted delivery of effective agents in the form of antibody-drug conjugates (Baldwin, in Monoclonal antibody therapy of human cancer, p. 23 (1985) or as bifunctional antibodies (Staerz and Bevan, PNAS 83:1453 (1986)).

Summary of the Invention

This invention pertains to immunoreactive heterochain antibody (immunoglobulin) . The hetero¬ chain antibody comprises at least one heavy chain variable region derived from a first antibody and at least one light chain variable region derived from a second antibody, the variable regions being as- sociated to form a functional antigen binding site. The antigen binding site of the heterochain antibody is a composite of the variable regions of the antibodies from which the heterochains are derived and consequently can exhibit different binding properties (e.g. different affinity or specificity) than the parent antibodies. This invention also pertains to methods of preparing heterochain anti¬ bodies and to methods of diagnosis and therapy of disease employing the heterochain antibodies.

Brief Description of the Figures

Figure 1 shows a restriction map of gene constructs for the production of heterochain anti¬ bodies (A) pSV2 Δ Hgptl7-lAV _H -hC _γ3 and (B) pSVneoGA73.3 _κ mC _κ .

Figure 2 shows a schematic illustration of the three types of IgG secreted by the HC1.1 cells. Solid lines represent the chimeric 17-1A heavy chain and broken lines represent Ig peptides of murine GA73.3 origin.

Figure 3 shows SDS/polyacrylamide gel analysis of IgG produced by HC1.1 cells. Three μg of purified IgG was electrophoresed on a SDS/12% polyacrylamide gel under reducing conditions. Lane 1 chimeric 17-1A G3K; lane 2, GA73.3; lane 3, HC1.2; HC1.1 before (lane 4) and after (lane 5) removal of GA73.3-derived heavy chain on Sepharose- bound anti-mouse IgG (Fc) antibody.

Figure 4 shows the binding of iodinated mono- clonal antibody (MAb) to HT29 cells in the presence of purified IgG of GA73.3 (o- ©) , HC1.1 (α π) ,

HC1.2 (. •) , and 17-1A (Λ Δ) . Iodinated MAb used is HC1.2 (A), GA73.3 (B) , and 17-1A (C) .

Figure 5 shows a Southern blot analysis. Ten -3 of genomic DNA was digested with Hindlll, frac¬ tionated on a 0.7% agarose, and transferred to nitrocellulose. Bound DNA was hybridized with the mouse J _κ probe. The 2.7 kb Hindlll restriction fragment from the mouse germline J _κ locus is shown below the autoradiogram. Lane 1, mouse liver DNA as a germline control; lane 2, 653, a mouse myeloma cell line; lane 3, GA73.3, a hybridoma cell line derived by using 653 as a fusion partner.

Detailed Description of the Invention

This invention pertains to immunoreactive heterochain antibodies and to methods of their preparation and use. The heterochain antibodies are made up of at least one light chain and at least one heavy chain derived from different antibodies. The heterochains are associated to form a functional antigen binding region. The combined chains form unique antigen binding region where one of the variable regions is derived from a first antibody and the other variable region is derived from a second antibody.

The heterochain antibodies of this invention can have different antigen or binding properties than the parent antibodies from which they are derived. For example, the antibodies can have different epitopic specificity or affinity than the parent antibody. In preferred embodiments the heterochain antibodies are derived from parent antibodies reactive with the same antigen or the same epitope (or related epitope) . The invention provides a means for altering antibody binding characteristics to provide improved antibodies for specific applications in diagnosis and therapy. The heterochain antibodies of this invention can be monovalent, divalent or polyvalent. Monovalent are dimers (HL) formed of a heavy chain from a first antibody associated through interchain disulfide bridges with a light chain from a second antibody. Divalent antibodies are tetramers (H_L ) formed of two associated dimers. Polyvalent anti¬ bodies can be produced, for example, by employing

heavy chains which aggregate (e.g. heavy chains having mu type constant regions or aggregatable portions thereof) .

The individual heterochains of the heterochain antibody can be chimeric chains where the variable region of the chain is derived from one animal species and the constant region of the chain is derived from a second animal species. For example, a murine/human chimeric chain can comprise a variable region derived from a ^' murine antibody and a region derived from a human antibody. It is par¬ ticularly advantageous to employ human constant regions to produce heterochain antibodies for in vivo use in humans because this reduces the risk of deleterious immune reactions in humans against the administered antibody. Further, human-derived constant regions are expected to have better ef¬ fector function and longer circulating half-lives in humans. The heterochains can also be truncated chains (truncated at the carboxyl end) . For example, truncated heavy chains can be used to produce heterochain antibody fragments analogous to the antibody fragments Fv, Fab, Fab ¹ or F(ab')_ which are produced by enzyme cleavage. Heterochain antibodies having truncated chains are particularly useful for immunoscintigraphic procedures.

The heavy chains of the heterochain antibody can have constant regions selected from any of the five isotypes alpha, delta, epsilon, gamma or mu. In addition, heavy chains may be of various

subclasses (such as the IgG subclasses) . The different classes and subclasses of heavy chains provide for different antibody effector functions and, thus by choosing the desired heavy chain constant region, a heterochain antibody with a desired effector function can be produced. The light chains can have either a kappa or lambda constant chain.

Antibody heterochains for production can be derived from a variety of sources. Variable regions from antibodies against tumor-associated antigens can be combined to produce novel heterochain anti¬ bodies which have unique binding characteristics. Among the anti-tumor antibodies from which variable regions can be derived are antibodies specific for antigens associated with gastrointestinal (e.g. colon and pancreas) , breast, ovarian, lung and renal cancer. Some specific examples of anti-tumor antibodies are 17-1A (gastrointestinal tumors) , OC 125 (ovarian carcinoma) , OV-TL3 (ovarian carcinoma) , 103D2 (breast) and 123.C3 (renal carcinoma). Variable region may also be derived from antibody against an antigen associated with cardiovascular disease (such as anti-myosin antibody and antibody reactive with artherosclerotic plaques) , antibody against infectious agents (such as antibody against bacterial toxins (e.g. gram-negative lipopolysaccha- ride) and viral antigens) and antibody against components or a thrombrus (e.g. anti-fibrin anti- body, anti-platelet antibody) .

Heterochain antibodies of this invention can be made by several different methods. In one method, cells capable of producing a first antibody are transfected with nucleic acid (DNA or RNA) which encodes a heavy and/or light chain of a second antibody to yield transfected cell capable of expressing first and second antibody chains. The transfected cells are cloned and the resulting clones are selected for the ability to produce heterochain antibody. Suitable cells for trans- fection and production of heterochain antibody are lymphoid cells which are capable of producing a desired first antibody e.g., transfected B lympho¬ cytes and hybridoma cells. Lymphoid cells which normally produce antibody possess the required cellular machinery for glycosylation and assembly of the heterochain antibodies.

In a preferred method of producing heterochain antibody, hybridoma cells which have lost the ability to produce one of the chains of the antibody which they normally produce (called chain-loss variants) are employed. In these cells, production of the normal homochain antibody is eliminated. The cells are transfected with DNA (or RNA) encoding a heterochain which replaces the light or heavy chain which the recipient cell has lost the capability to produce and which has the desired variable region. Transfected hybridomas are selected for production of the heterochain antibody. Hybridoma cells which are unable to produce an antibody chain can be selected by soft-agar cloning and screening with

appropriate antiserum (Springer, et al. Eur. J. Immunol. 8 _^ :539 (1978)).

In another method for producing heterochain antibody, antibody-producing cells which have lost capability to produce both antibody chains are used. In this case, the nonproducing cell is transfected with nucleic acid (DNA or RNA) coding for both the heavy chain and the light chain of the heterochain antibody. Each encodes a variable region of a different antibody. Transfected cells are selected for products of the heterochain antibody. For this method, hybridoma or myeloma cells which are in¬ capable of producing either antibody are available. An example is the non-producing myeloma cell line Sp2/0 (Shulman et al. , Nature 276:269 (1978)). According to the methods of this invention appropriate recipient cells are transfected with nucleic acid constructs, preferably DNA, encoding the desired antibody heterochain or heterochains. In general, DNA constructs for each of the light and heavy chains components of the heterochain antibody comprise a fused gene comprising a first DNA segment which encodes at least the functional portion of the variable region linked to a second DNA segment encoding at least a part of a constant region. The fused gene is assembled in or inserted into a vector for transfection of the appropriate recipient cells.

In preferred embodiments the fused gene con¬ struct will comprise a functionally rearranged gene encoding a variable region for an antibody chain linked to a gene encoding a constant region of an

antibody chain. The construct will also include the endogenous promoter and enhancer for the variable region encoding gene. For example, the variable region encoding genes can be obtained as a DNA fragment comprising the leader peptide, the VJ gene (functionally rearranged variable (V) regions with joining (J) segment) and the endogenous promoter and enhancer for these genes. This fragment is linked to a DNA fragment containing the gene encoding the desired constant region (or a truncated portion thereof) .

Genes encoding antibody light and heavy-chains can be obtained from lymphoid cells which produce the antibodies specific for the desired antigen or epitope. Many antibody producing cell lines are presently avaiable. For example, available hybri¬ doma cell lines which produce antibody against tumor associated antigens (e.g. 17-1A, CA125 and others) provide a source of immunoglobulin variable region genes against tumor-associated antigens. Hybridoma cell lines producing antibody against a desired antigen can be made by standard procedures of Kohler and Milstein, e.g. challenging a rodent with a desired antigen, forming fused hybrid cells between antibody producing cells and a myeloma cloning the hybrid and selecting clones which produce antibody against the antigen. Techniques for production of hybridoma cells which produce antibody against tumor-associated antigens are also known in the art. See e.g., U.S. Patent No. 4,172,124 Koprowski et al.

Constant regions can be obtained from antibody- producing cells by standard cloning techniques. Human constant regions are most preferred. Because genes representing the two classes of human light chains and the five classes of human heavy chains have been cloned, constant regions of human origin are readily available from these clones.

The fused genes encoding either the light or heavy chains are assembled into expression vectors which can be used to transfect a recipient cell.

Suitable vectors for the heterochain gene constructs include plasmids of the types pBR322, pEMBL, and pUC. The introduction of the heterochain gene constructs into plasmid vectors to produce recom- binant plasmids can be accomplished by standard procedures of eukaryotic cell transfection. In preferred embodiments, the vector contains two selectable genes-one for selection in a bacterial system and one for selection in a eukaryotic system. These vectors allow production and amplification of the fused genes in bacterial systems and subsequent transfection of eukaryotic cells and selection of transfected cells. Examples of selectable gene for the bacterial system are the genes which confer ampicillin resistance and the gene which confers chloramphenicol resistance. Two selectable genes for selection of eukaryotic transfectants are preferred: (i) the xanthine-guanine phosphoribosyl- transferase gene (gpt) , and (ii) the phosphotrans- ferase gene from Tn5 (designated neo) . Selection with gpt is based on the ability of the enzyme

encoded by this gene to use xanthine as a substrate for purine nucleotide synthesis; the analogous endogenous enzyme cannot. In a medium containing xanthine and mycophenolic acid which blocks the conversion of inosine monophosphate to xanthine monophosphate, only cells expressing the gpt gene can survive. The product of the neo blocks the inhibition of protein synthesis in eukaryotic cells caused by the antibiotic G418 and other antibiotics of its class.

In methods where cells are to be cotransfected with genes encoding both the light and heavy chains of the heterochain antibody, the genes are preferably assembled in two different expression vectors which can be used to cotransfect a recipient cell. In this case, each vector contains a dif¬ ferent selectable gene for eukaryotic transfeetants. This allows cotransfection of the recipient cell and selection of cotransfected cells (i.e. cells that have received both vectors) . Selection of cotrans¬ fected cells is accomplished by selection for both selectable markers, which can be done simultaneously or sequentially.

Several methods exist for transfecting lymphoid cells such as hybridoma and myeloma cells with vectors containing antibody chain encoding genes. A preferred way of introducing DNA into lymphoid cells is by electroporation. In the procedure recipient cells are subjected to an electric pulse in the presence of the DNA to be incorporated in to the cell. See e.g. Potter, et al. PNAS 81:7161 (1984).

Another way to introduce DNA is by protoplast fusion. In this method, lysozy e is used to strip cell walls from bacteria harboring the recombinant plasmid containing the heterochain Ig gene to produce spheroplasts. The resulting spheroplasts are fused with the lymphoid cells in the presence of polyethylene glycol. After protoplast fusion, the transfectants are selected and isolated. (Oi, et al. , PNAS 8JD:825 (1983)). Other techniques which can be used to introduce DNA into many cell types are calcium phosphate precipitation (Graham and van der Eb, Virology 52:456 (1973) and DEAE-dextran (Cullen, et al. , Nature 307:241 (1984)).

The heterochain antibody genes can be expressed in nonlymphoid cells such as bacteria or yeast.

When expressed in bacteria, the immunoglobulin heavy chains and light chains become part of inclusion bodies. Thus, the chains must be isolated and purified and then assembled into functional immuno- globulin molecules. The yield of heterochain antibody in bacteria may be low because bacteria lack the ability to glcosylate the expressed anti¬ body chains.

The heterochain antibodies of this invention can be used in methods of diagnosis and therapy.

The heterochain antibodies can be used iτι vitro in diagnostic tests such as immunoassay, as contrast agents (labeled heterochain antibodies) for in vivo imaging based upon a targeted antigen (e.g. immuno- scintigraphy of tumor, myocardial infarct, arthero- scelerotic plaque and thrombus) , tumor therapy and prophylaxis and/or therapy of infectious diseases.

This invention is illustrated further by the following samples of the production of heterochain antibody HC1 containing chimeric (murine/human) 17-1A heavy chain and the murine GA73.3 light chain.

EXAMPLES

Example 1. Transfection of chimeric 17-1A heavy chain gene into GA73.3 cells.

A. Construction of expression vector containing the chimeric 17-1A heavy chain gene: pSV2ΔHgptl7- lAVH-hC ₇₃ .

The functionally rearranged heavy chain variable gene of 17-1A was isolated and cloned into an expression vector containing a genomic DNA fragment encoding human i _ constant region to give pSV2Δ Hgptl7-lAVH-hC _?3 as described (Sun et al. , PNAS j$4_:214 (1987)). The restriction map is shown in Figure IA. Transfection of myeloma cells with this gene construct, together with an expression vector containing the 17-IA chimeric light chain, resulted in the production of functional IgG that retained the specific binding to the surface antigen 17-1A expressed on human colorectal carcinoma SW1116 cells.

B. Transfection of pSV2 Δ Hgptl7-lAVH-hC into GA73.3 cells.

DNA was transfected into GA73.3 cells by electro- poration using Bio-Rad Gene Pulsar Transfection Apparatus. Approximately 8 X 10 cells in 0.8 ml of Dulbecco's phosphate-buffered saline (PBS) con- taining 30 μg of pSV2 Δ Hgptl7-lAVH-hC were subjected to an electric field of 0.15 kV and a capacitance of 960 μFD at 4°. The cells were diluted with MEM medium supplemented with 15% FBS and plated out in a 96-well microtiter plate. After 48 hr, the medium was changed to MEM containing 0.5 Ug/ml mycophenolic acid, 50 jug/ml xanthine, and 2.5 μg/ml hypoxanthine for selection of transfected cells.

C. Antibody production and purification.

stably transfected cell line HCl.l was established and analyzed. The HCl.l cells synthesized the mouse/human chimeric heavy chain in addition to the endogenous murine GA73.3 heavy and light chains. Since only the tetrameric antibody consisting of two heavy and two light chains can be secreted, there are three possible types of IgG in the culture supernatant, as illustrated in Figure 2. Tissue culture supernatant was analyzed for IgG content by particle concentration fluorescence immunoassay using standard curves generated with purified human or mouse IgG. The assays were carried out with an automated instrument (Pandex Laboratories, Mundelein, IL) . Concentration of IgG production was ^" estimated

to be 8 μg/ml by using polystyrene beads coated with goat anti-mouse IgG(Fc) antibody and fluorescein- conjugated goat anti-mouse IgG(Fc) antibody. This concentration represented the total amount of both IgG types B and C. When goat anti-human IgG(Fc) antibody coated polystyrene beads and its fluo- rescein conjugated probe were used in the assays with the same culture supernatant, the IgG content was estimated to be 1.2 μg/ml for IgG types A and B. IgG A and B were separated from C using an affinity column containing Sepharose-bound anti human IgG(Fc) antibody which specifically bound the human IgG(Fc) portion of IgG A and B. The eluate, when checked on reducing SDS/PAGE analysis, showed a homogenous light chain band and two heavy chain bands (Figure 3) , corresponding to the chimeric 17-1A heavy chain and the murine GA73.3 heavy chain. Mixture of A and B was then passed through a Sepharose-bound anti mouse IgG(Fc) antibody column to remove IgG B. The relative amounts of the chimeric to the murine heavy chains was about 100:1 after two column purifica¬ tions as determined on Pandex using appropriate reagents. The resulting purified heterochain antibody, HCl.l, was used for HT29 cell binding assay.

D. Immunoactivity analysis of HCl.l using human colorectal carcinoma HT29 cells.

Sixty ug of purified IgG type A (HC1.2, see example

2) was labelled with Iodo-Beads (Pierce Chemical Company, Rockford, IL) using 1 mCi of Na 125I. After

10 min, the reaction was quenched with PBS con¬ taining 5% BSA. Free iodine was removed with a prepacked PD-10 column (Pharmacia) . Specific activity was typically 10,000 cpm/ng of protein. Human colorectal carcinoma HT29 cell line was grown in MEM supplemented with 10% FBS. Cells were harvested with trypsin, washed, and resuspended in growth medium. Cells were then seeded into 96-well microtiter plates at 2 X 10 5 cells per well m 100 μl aliquots. After incubation at 37° for 17 hr, the cells .were refed with 100 μl of cold medium, and kept at 4° for 0.5 hr. Each well was then incubated at 4° for 2 hr with medium containing 400,000 cpm of iodinated HC1.2 and different concentrations of cold competing IgG in a final volume of 100 μl. The cells were then washed 3 times with warm medium and cell-bound radioactivity was measured in a gamma- counter. Curves for binding of iodinated HC1.2 to HT29 cells as competed by different concentrations of purified IgG are shown in Figure 4A. The concen¬ trations required to inhibit 50% of the iodinated- HC1.2 binding (IC50) are estimated to be 1.3, 5.5, and 45 μg/ml for MAb GA73.3, HCl.l and 17-1A, respectively. The results indicated that HCl.l bound to the surface antigen of HT29 cells with different affinity or specificity when compared to MAb GA73.3 and 17-1A.

Example 2. Transfection of chimeric 17-1A heavy chain gene into GA73.3L cells.

A. Use of a heavy chain-loss variant of GA73.3, GA73.3L.

In example 1, the transfection of a heavy chain gene into GA73.3 cells, and the two column-purification of the desired heterochain antibody (Fig. 2A) from the other possible IgG (Fig. 2B, 2C) present in the culture supernatant of the HCl.l cells is described. To generate a cell line that produced only the heterochain antibody, we used GA73.3L as the re- cipient cell line for the heavy chain gene, pSV2Δ Hgptl7-lAVH-hC - . GA73.3L is a heavy chain-loss variant that lost the ability to synthesize the heavy chain. Heavy chain-loss variant cell lines can be selected according to published protocols. GA73.3L, however, occurred naturally during con- tinuous cell culture. GA73.3L cells ceased produc¬ tion of the heavy chain but retained the ability to synthesize the light chain in the cytoplasm. Upon transfection with pSV2 Hgptl7-lAVH-hC , the GA73.3L cells secreted a monoclonal antibody containing the chimeric 17-1A heavy chain and the murine GA73.3 light chain.

B. Transfection of p ^~ ~SV2 H17-lAVH-hC73., into GA73.3L cells.

DNA was transfected into the GA73.3L cells by the method of electroporation as described in section B of example 1. Forty-eight hours after electropora¬ tion, cells were selected in growth medium (DMEM

supplemented with 15% FBS) containing 0.5 μg/ml of mycophenolic acid, 50 μg/ml of xanthine, and 2.5 μg/ml of hypoxanthine.

C. Antibody production and purification.

A stably transfected cell line HC1.2 was established and analyzed. Tissue culture supernatant was analyzed for IgG content using the same method as described in section C of example 1. Concentration of IgG production was estimated to be 8 μg/ml by using polystyrene beads coated with goat anti-human IgG(Fc) antibody and fluorescein-conjugated goat anti-human IgG(Fc) antibody. Heterochain antibody HC1.2 was purified on an affinity column of Sepharose-bound goat anti-human IgG(Fc) antibody. The purified IgG showed two bands corresponding to the 17-IA chimeric heavy chain and the murine GA73.3 light chain on reducing SDS/PAGE analysis (Figure 3) , and therefore had a structure corresponding to Figure 2A.

D _* Immunoactivity analysis of MAb HC1.2 using HT29 cells.

The cell binding assays were done according to section D of example 1. Curve for binding of iodinated HC1.2 to HT29 cells as competed by dif- ferent concentrations of MAb HC1.2 is shown in

Figure 4A. The concentration required to inhibit 50% of the iodinated-HC1.2 is estimated to be 5.0

μg/ml. Competitive HT29 cell binding assays were also carried out using iodinated MAb GA73.3 (Figure 4B) and MAb 17-1A (Figure 4C) . IC50 values were estimated from binding curves and summarized in Table 1. When iodinated GA73.3 was used, IC50 values of GA73.3, HCl.2, and 17-1A were 4.2, 42 and 4000 μg/ml, respectively. When iodinated 17-1A was used, IC50 values of GA73.3, HCl.2 and 17-1A were 0.45, 0.96 and 3.0 jUg/ml, respectively. In each case, the IC50 values of HCl.2 were different than either of GA73.3 and 17-1A. The results indicated that MAb HCl.2 had different binding affinity than the two antibody from which its heavy and light chains were derived. It is also possible that the heterochain antibody recognized a different epitope of the surface antigen of HT29 cells.

Table 1. Summary of IC50 values (μg/ml) in competitive binding assays using HT29 cells.

iodinated MAb com etin MAb

Example 3. An alternative method to produce a cell line that secrets the heterochain antibody HCl is to transfect both the heavy and light chain genes into a mouse myeloma cell line. This approach requires

the cloning of the functionally rearranged light chain variable region gene of the GA73.3 cells. The light chain variable gene is then cloned into • expression vector containing the mouse K constant region gene. Transfection of this light chain gene construct together with pSV2ΔHgptl7-lAV _H ~hC ₃ into nonproducing mouse myeloma SP2/0 cells allows selection of stable cell lines producing heterochain antibody consisting chimeric 17-IA heavy chain and GA73.3 light chain.

A. Cloning of the functionally rearranged light chain variable gene of GA73.3 and construction of the expression vector.

Figure 5 shows the Southern analysis of re- arranged fragments cont ininc__J sequences._ DNA of GA73.3 cells gave a rearranged Hindlll fragment of 4.6 kb which was different from the 6.5 kb band characteristic of the fusion partner, 653. This 4.6 kb Hindlll DNA fragment thus contains the functional VJ rearrangement in the « light chain locus of the GA73.3 cells. A partial genomic library can be constructed with enriched DNA fragments of appro¬ priate sizes using phage vector such as Charon 27 or Lambda ZAP (Stratagene, San Diego, CA) . Alter- natively, a complete genomic library can be con¬ structed using EMBL-3A phage vectors (Stratagene) . The recombinant plaques of genomic libraries are screened with the mouse J _κ probe using standard techniques (Maniatis, et al. Molecular Cloning: A

Laboratory Manual p316 (1982)). The 4.6 kb Hindlll fragment is then joined to the genomic DNA segment encoding mouse constant region in an expression vector containing a dominant selectable marker, neo. The restriction map of this light chain gene con¬ struct, pSVneoGA73.3V, -mC , is shown in Figure IB.

B. Transfection of the heavy and light chain constructs into mouse myeloma Sp2/0 cells.

The heavy and light chain vectors shown in Figure 1, pSV2.Hgptl7-lAVH-hC 7J_ and pSVneo GA73.3V i -mC K . are used to transfect the nonproducing mouse myeloma Sp2/0 cells. The heavy chain vector contains the gpt gene and the light chain vector contains the neo gene, it is therefore possible to select for cells that have integrated these two vectors by growing transfected cells in medium containing both myco- phenolic acid and the antibiotic G418. Transfer of DNA into Sp2/0 cells is carried out by electro¬ poration as in section B of example 1. The optimal electric field is 0.2 kV for Sp2/0 cells. Forty- eight hr after electroporation, the transfected cells are selected in growth medium (DMEM sup¬ plemented with 15% FBS) containing 1 μg/ml of mycophenolic acid, 50 μg/ml of xanthine, 2.5 μg/ml of hypoxanthine, and 1 mg/ l of G418. Stable transfected cell lines are established and analyzed..

C. Antibody characterization and purification, immunoactivity analysis are done according to section C and D of example 2.

Equivalents Those skilled in the art will recognize, or be able to ascertain using no more than routine ex¬ perimentation, many equivalents to the specific embodiments of the invention described herein. Such equivalents are intended to be encompassed by the following claims.