Title:
IMPROVED GLUCOSE DEHYDROGENASE POLYPEPTIDES AND RELATED POLYNUCLEOTIDES
Document Type and Number:
WIPO Patent Application WO2005045016
Kind Code:
A3
Abstract:
The present invention is directed to glucose dehydrogenase (GDH) polypeptides that have enhanced GDH activity and/or thermostability relative to the backbone wild-type glucose dehydrogenase polypeptide. In addition, the present invention is directed to a plynucleotide that encodes for the GDH polypeptides of the present invention, to nucleic acid sequences comrpising the polynucleotides, to expression vectors comprising the polynucleotides operatively linked to a promoter, to host cells transformed to express the GDH polypeptides, and to a method for producing the GDH polypeptides of the present invention.
Inventors:
KREBBER ANKE
NEWMAN LISA MARIE
DAVIS S CHRISTOPHER
JENNE STEPHANE J
NEWMAN LISA MARIE
DAVIS S CHRISTOPHER
JENNE STEPHANE J
Application Number:
PCT/US2004/026194
Publication Date:
November 10, 2005
Filing Date:
August 11, 2004
Export Citation:
Assignee:
CODEXIS INC (US)
International Classes:
C12N9/04; (IPC1-7): C12N9/04; C12N15/52; C12N15/63; C12N5/10
Domestic Patent References:
| WO2004015132A2 | 2004-02-19 |
Other References:
LAMPEL K A ET AL: "CHARACTERIZATION OF THE DEVELOPMENTALLY REGULATED BACILLUS-SUBTILIS GLUCOSE DEHYDROGENASE GENE", JOURNAL OF BACTERIOLOGY, vol. 166, no. 1, 1986, pages 238 - 243, XP002327631, ISSN: 0021-9193
MANJON A ET AL: "Increased activity of glucose dehydrogenase co-immobilized with a redox mediator in a bioreactor with electrochemical NAD+ regeneration", BIOTECHNOLOGY LETTERS, vol. 24, no. 15, August 2002 (2002-08-01), pages 1227 - 1232, XP002327599, ISSN: 0141-5492
MANJON A ET AL: "Increased activity of glucose dehydrogenase co-immobilized with a redox mediator in a bioreactor with electrochemical NAD+ regeneration", BIOTECHNOLOGY LETTERS, vol. 24, no. 15, August 2002 (2002-08-01), pages 1227 - 1232, XP002327599, ISSN: 0141-5492
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