Title:
IMPROVED PROMOTER AND CARRIER COMPOSED OF SAME AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2019/128837
Kind Code:
A1
Abstract:
An improved promoter and application thereof; the improvement refers to the mutation of a nucleotide sequence, in the promoter area, between -35 area to -10 area into an endonuclease recognition site. The improvement may overcome the problem existing in blue-white screening vectors that poisonousness may be produced to the host, which leads to clone incapability, when transcription or translation product of a foreign gene is promoted by a strong promoter. Thus, the defect of false positive clone resulted from gene frame shift mutation which is caused by the deficiency of the vector of 1-2bp at the restriction enzyme cutting site can be avoided, and the false negative phenomenon of a panel being full of locus coeruleus due to smaller foreign DNA fragments and the insertion of foreign DNA not changing the reading frame of the gene can be eliminated.
Inventors:
XUE GAOXU (CN)
JIA YANKAI (CN)
QI TIANMING (CN)
FENG AIHUA (CN)
XIE ZHENGLI (CN)
WU XIN (CN)
SUN ZHONGPING (CN)
LIAO GUOJUAN (CN)
JIA YANKAI (CN)
QI TIANMING (CN)
FENG AIHUA (CN)
XIE ZHENGLI (CN)
WU XIN (CN)
SUN ZHONGPING (CN)
LIAO GUOJUAN (CN)
Application Number:
PCT/CN2018/122312
Publication Date:
July 04, 2019
Filing Date:
December 20, 2018
Export Citation:
Assignee:
GENEWIZ INC SZ (CN)
International Classes:
C12N15/113; C12N1/21; C12N15/66; C12N15/70; C12R1/19
Domestic Patent References:
WO2000022112A1 | 2000-04-20 |
Foreign References:
CN108118059A | 2018-06-05 | |||
CN108165551A | 2018-06-15 | |||
CN108060168A | 2018-05-22 | |||
CN108118058A | 2018-06-05 | |||
CN108130338A | 2018-06-08 | |||
CN108588102A | 2018-09-28 | |||
CN1233287A | 1999-10-27 | |||
CN104513830A | 2015-04-15 | |||
CN107058316A | 2017-08-18 | |||
CN1425071A | 2003-06-18 | |||
CN105907632A | 2016-08-31 | |||
CN105400809A | 2016-03-16 |
Other References:
SHEN XINGZHONG: "Modification and Expression of LipA Promoter in Bacillus Subtilis", CHINESE MASTER'S THESES FULL-TEXT DATABASE , 15 September 2009 (2009-09-15), pages 1 - 55, XP055718476, ISSN: 1674-0246
FEISHU HU: "Associations between hepatitis B virus basal core promo- ter/pre-core region mutations and the risk of acute-on-chronic liver failu- re: a meta-analysis", VIROLOGY JOURNAL, vol. 12, no. 1, 11 June 2015 (2015-06-11), pages 87, XP055622476, ISSN: 1743-422X
HU L-LZHANG S-SLI X-XWANG B-L, MOLECULAR BIOLOGY, vol. 44, no. 1, 2010, pages 161 - 4
MIYAZAKI K, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, vol. 110, no. 3, September 2010 (2010-09-01), pages 372 - 3
See also references of EP 3733851A4
FEISHU HU: "Associations between hepatitis B virus basal core promo- ter/pre-core region mutations and the risk of acute-on-chronic liver failu- re: a meta-analysis", VIROLOGY JOURNAL, vol. 12, no. 1, 11 June 2015 (2015-06-11), pages 87, XP055622476, ISSN: 1743-422X
HU L-LZHANG S-SLI X-XWANG B-L, MOLECULAR BIOLOGY, vol. 44, no. 1, 2010, pages 161 - 4
MIYAZAKI K, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, vol. 110, no. 3, September 2010 (2010-09-01), pages 372 - 3
See also references of EP 3733851A4
Attorney, Agent or Firm:
BEYOND ATTORNEYS AT LAW (CN)
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