Title:
IN-GEL FLUORESCENT PROTEIN STAINING TECHNIQUE
Document Type and Number:
WIPO Patent Application WO2004038423
Kind Code:
A3
Abstract:
An "in-gel" staining technique for detecting and/or separating proteins during electrophoresis, illustratively as a modification of the standard Laemmli procedure. A fluorescent dye, such as Nile red or Phosphine, is included in the running buffer ( mobile phase) which may be a standard Laemmli Tris-Glycine SDS buffer that has been modified to reduce the concentration of detergent (SDS) to less than the typical concentration (0.10%v/v). The fluorescent dye stains proteins during electrophoretic separation. The post-electrophoretic operations are, therefor, reduced and the separated, stained fractions are recoverable for further processing, purifying or analysis.
Inventors:
KITZLER JEFFREY (US)
WERNER DAVID (US)
IBRAHIM ABDUL (US)
WERNER DAVID (US)
IBRAHIM ABDUL (US)
Application Number:
PCT/US2003/033819
Publication Date:
June 10, 2004
Filing Date:
October 23, 2003
Export Citation:
Assignee:
NAT DIAGNOSTICS INC (US)
KITZLER JEFFREY (US)
WERNER DAVID (US)
IBRAHIM ABDUL (US)
KITZLER JEFFREY (US)
WERNER DAVID (US)
IBRAHIM ABDUL (US)
International Classes:
G01N27/447; (IPC1-7): G01N33/48; G01N27/447; G01N33/52
Foreign References:
| US5616502A | 1997-04-01 | |||
| US6579718B1 | 2003-06-17 | |||
| US6277259B1 | 2001-08-21 |
Other References:
ALBA F JAVIER ET AL: "Detection of five nanograms of protein by two-minute Nile red staining of unfixed SDS gels", BIOTECHNIQUES, EATON PUBLISHING, NATICK, US, vol. 21, no. 4, 1996, pages 625 - 626, XP002181429, ISSN: 0736-6205
GREENSPAN P. ET AL.: "Detection by Nile Red of agarose gel electrophoresed native and modified low density lipoprotein", ELECTROPHORESIS, vol. 14, no. 1-2, 1993, Weinheim, pages 65 - 68, XP009029458
ALBA, JF; ET AL.: "Rapid fluorescent monitoring of total protein patterns on sodium dodecyl sulfate-polyacrylamide gels and Western blots before immunodetection and sequencing", ELECTROPHORESIS, vol. 19, no. 14, October 1998 (1998-10-01), Weinheim, pages 2407 - 2411, XP009029459
GREENSPAN P. ET AL.: "Detection by Nile Red of agarose gel electrophoresed native and modified low density lipoprotein", ELECTROPHORESIS, vol. 14, no. 1-2, 1993, Weinheim, pages 65 - 68, XP009029458
ALBA, JF; ET AL.: "Rapid fluorescent monitoring of total protein patterns on sodium dodecyl sulfate-polyacrylamide gels and Western blots before immunodetection and sequencing", ELECTROPHORESIS, vol. 19, no. 14, October 1998 (1998-10-01), Weinheim, pages 2407 - 2411, XP009029459
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