Title:
INDUCIBLE CONTROL OF GENE EXPRESSION
Document Type and Number:
WIPO Patent Application WO/2022/020431
Kind Code:
A3
Abstract:
The technology described herein is directed to inducible and repressible polypeptides and polypeptide systems. In particular, described herein are split sequence-specific nucleases and split recombinases that are linked to drug-inducible or drug-repressible dimerization domains. In some embodiments, the polypeptides comprise sequestering domains and/or have their expression controlled by an inducible promoter. In multiple aspects described herein are polynucleotides, vectors, cells, and pharmaceutical compositions comprising said polypeptides or polypeptide systems. Also described herein are methods of using said polypeptide systems to modulate the expression of a target polypeptide or to treat a subject in need of a cell therapy.
Inventors:
WONG WILSON (US)
DING YAGE (US)
DING YAGE (US)
Application Number:
PCT/US2021/042515
Publication Date:
March 10, 2022
Filing Date:
July 21, 2021
Export Citation:
Assignee:
UNIV BOSTON (US)
International Classes:
C12N9/22; C07K19/00; C12N9/90; C12N15/63; C12N15/79
Domestic Patent References:
| WO2019236982A1 | 2019-12-12 | |||
| WO2019060746A1 | 2019-03-28 |
Foreign References:
| US20180066242A1 | 2018-03-08 |
Other References:
BOJAR DANIEL, SCHELLER LEO, HAMRI GHISLAINE CHARPIN-EL, XIE MINGQI, FUSSENEGGER MARTIN: "Caffeine-inducible gene switches controlling experimental diabetes", NATURE COMMUNICATIONS, vol. 9, no. 1, 1 December 2018 (2018-12-01), XP055786426, DOI: 10.1038/s41467-018-04744-1
ZHANG BO, YE YANGMIAO, YE WEIWEI, PERČULIJA VANJA, JIANG HAN, CHEN YIYANG, LI YU, CHEN JING, LIN JINYING, WANG SIQI, CHEN QI, HAN : "Two HEPN domains dictate CRISPR RNA maturation and target cleavage in Cas13d", NATURE COMMUNICATIONS, NATURE PUBLISHING GROUP, vol. 10, no. 1, 1 December 2019 (2019-12-01), pages 2544, XP055915355, DOI: 10.1038/s41467-019-10507-3
ZHANG BO, YE YANGMIAO, YE WEIWEI, PERČULIJA VANJA, JIANG HAN, CHEN YIYANG, LI YU, CHEN JING, LIN JINYING, WANG SIQI, CHEN QI, HAN : "Two HEPN domains dictate CRISPR RNA maturation and target cleavage in Cas13d", NATURE COMMUNICATIONS, NATURE PUBLISHING GROUP, vol. 10, no. 1, 1 December 2019 (2019-12-01), pages 2544, XP055915355, DOI: 10.1038/s41467-019-10507-3
Attorney, Agent or Firm:
KLING, Nicole D. et al. (US)
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