Title:
INFORMATION ACQUISITION DEVICE, PROGRAM, RECOMBINANT VECTOR PRODUCTION METHOD, FISSION YEAST, AND GENE EXPRESSION SUPPRESSING METHOD
Document Type and Number:
WIPO Patent Application WO/2023/063340
Kind Code:
A1
Abstract:
An information acquisition device (100) acquires a target sequence, which is a target of a guide RNA introduced into fission yeast together with enzymatically inactive dCas9 protein so as to suppress expression of a target gene, the information acquisition device (100) comprising an acquisition unit (1) for acquiring, among an upstream target sequence adjacent to the PAM sequence in genes of fission yeast, a target sequence in which an intermediate base is positioned within a region including 30 bases located upstream from the transcription initiation point of the target gene and 150 bases located downstream from the transcription initiation point, wherein when the target sequence is formed of an odd number n of bases, the 0.5(n+1)-th base from the 5' terminal is taken as the intermediate base; and when the target sequence is formed of an even number m of bases, the 0.5m-th or 0.5(m+1)-th base from the 5' terminal is taken as the intermediate base.
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Inventors:
ISHIKAWA KEN (JP)
SAITOH SHIGEAKI (JP)
SAITOH SHIGEAKI (JP)
Application Number:
PCT/JP2022/037999
Publication Date:
April 20, 2023
Filing Date:
October 12, 2022
Export Citation:
Assignee:
UNIV KURUME (JP)
International Classes:
C12M1/00; C12N1/19; C12N15/09; C12N15/11; C12N15/81
Other References:
GILBERT LUKE A.; LARSON MATTHEW H.; MORSUT LEONARDO; LIU ZAIRAN; BRAR GLORIA A.; TORRES SANDRA E.; STERN-GINOS: "CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes", CELL, ELSEVIER, AMSTERDAM NL, vol. 154, no. 2, 18 July 2013 (2013-07-18), Amsterdam NL , pages 442 - 451, XP028680105, ISSN: 0092-8674, DOI: 10.1016/j.cell.2013.06.044
HANA RASCHMANOVá, ASTRID WENINGER, ANTON GLIEDER, KARIN KOVAR, THOMAS VOGL: "Implementing CRISPR-Cas technologies in conventional and non-conventional yeasts: Current state and future prospects", BIOTECHNOLOGY ADVANCES., ELSEVIER PUBLISHING, BARKING., GB, vol. 36, no. 3, 1 May 2018 (2018-05-01), GB , pages 641 - 665, XP055527665, ISSN: 0734-9750, DOI: 10.1016/j.biotechadv.2018.01.006
GILBERT LUKE A.; HORLBECK MAX A.; ADAMSON BRITT; VILLALTA JACQUELINE E.; CHEN YUWEN; WHITEHEAD EVAN H.; GUIMARAES CARLA; PANNING B: "Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation", CELL, ELSEVIER, AMSTERDAM NL, vol. 159, no. 3, 9 October 2014 (2014-10-09), Amsterdam NL , pages 647 - 661, XP029084856, ISSN: 0092-8674, DOI: 10.1016/j.cell.2014.09.029
ISHIKAWA KEN, SOEJIMA SAEKO, MASUDA FUMIE, SAITOH SHIGEAKI: "Implementation of dCas9-mediated CRISPRi in the fission yeast Schizosaccharomyces pombe", G3 GENES|GENOMES|GENETICS, vol. 11, no. 4, 15 April 2021 (2021-04-15), XP093056983, DOI: 10.1093/g3journal/jkab051
HANA RASCHMANOVá, ASTRID WENINGER, ANTON GLIEDER, KARIN KOVAR, THOMAS VOGL: "Implementing CRISPR-Cas technologies in conventional and non-conventional yeasts: Current state and future prospects", BIOTECHNOLOGY ADVANCES., ELSEVIER PUBLISHING, BARKING., GB, vol. 36, no. 3, 1 May 2018 (2018-05-01), GB , pages 641 - 665, XP055527665, ISSN: 0734-9750, DOI: 10.1016/j.biotechadv.2018.01.006
GILBERT LUKE A.; HORLBECK MAX A.; ADAMSON BRITT; VILLALTA JACQUELINE E.; CHEN YUWEN; WHITEHEAD EVAN H.; GUIMARAES CARLA; PANNING B: "Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation", CELL, ELSEVIER, AMSTERDAM NL, vol. 159, no. 3, 9 October 2014 (2014-10-09), Amsterdam NL , pages 647 - 661, XP029084856, ISSN: 0092-8674, DOI: 10.1016/j.cell.2014.09.029
ISHIKAWA KEN, SOEJIMA SAEKO, MASUDA FUMIE, SAITOH SHIGEAKI: "Implementation of dCas9-mediated CRISPRi in the fission yeast Schizosaccharomyces pombe", G3 GENES|GENOMES|GENETICS, vol. 11, no. 4, 15 April 2021 (2021-04-15), XP093056983, DOI: 10.1093/g3journal/jkab051
Attorney, Agent or Firm:
KIMURA Mitsuru (JP)
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