FIELD OF THE INVENTION

The present invention relates to the inhibition of exoprotein in an absorbent article, such as vaginal tampons and sanitary napkins. More particularly, the invention relates to the incorporation of ether compounds, amide compounds and amine compound either alone or in combination into such absorbent articles and these compounds effects on Gram positive bacteria.

BACKGROUND OF THE INVENTION Disposable absorbent devices for the absorption of human exudates are widely used. These disposable devices typically have a compressed mass of absorbent formed into the desired shape, which is typically dictated by the intended consumer use. In the area of a menstrual tampon, the device is intended to be inserted in a body cavity for absorption of the body fluids generally discharged during a woman's menstrual period.

There exists in the female body a complex process which maintains the vagina and physiologically related areas in a healthy state. In a female between the age of menarche and menopause, the normal vagina provides an ecosystem for a variety of microorganisms. Bacteria are the predominant type of microorganism present in the vagina; most women harbor about 10 ⁹ bacteria per gram of vaginal exudate. The bacterial flora of the vagina is comprised of both aerobic and anaerobic bacteria. The more commonly isolated bacteria are Lactobacillus species, corynebacteria, Gardnerella vaginalis, Staphylococcus species, Peptococcus species, aerobic and anaerobic Streptococcal species, and Bacteroides species. Other microorganisms that have been isolated from the vagina on occasion include yeast (Candida albjcans), protozoa (Trichomonas vaginalis). mycoplasma (Mvcoplasma horninis). chlamydia (Chlamydia trachomatis). and viruses (Herpes simplex). These latter organisms are generally associated with vaginitis or venereal disease, although they may be present in low numbers without causing symptoms.

Physiological, social and idiosyncratic factors affect the quantity and species of bacteria present in the vagina. Physiological factors include age, days of the menstrual cycle, and pregnancy. For example, vaginal flora present in the vagina throughout the menstrual cycle can include lactobacilli, corynebacterium, ureaplasma, and mycoplasma. Social and idiosyncratic factors include method of birth control, sexual practices, systemic disease (e.g. diabetes), and medication.

Bacterial proteins and metabolic products produced in the vagina can affect other microorganisms and the human host. For example, the vagina between menstrual periods is mildly acidic having a pH ranging from about 3.8 to about 4.5. This pH range is generally considered the most favorable condition for the maintenance of normal flora. At that pH, the vagina normally harbors the numerous species of microorganisms in a balanced ecology, playing a beneficial role in providing protection and resistance to infection and makes the vagina inhospitable to some species of bacteria such as Staphylococcus aureus (S. aureus). The low pH is a consequence of the growth of lactobacilli and their production of acidic products. Microorganisms in the vagina can also produce antimicrobial compounds such as hydrogen peroxide and bactericides directed at other bacterial species. One example is the lactocins, bacteriocin-like products of lactobacilli directed against other species of lactobacilli. Some microbial products may affect the human host. For example, S. aureus can produce and excrete into its environment a variety of exoproteins including enterotoxins, Toxic Shock Syndrome Toxin-1 (TSST-1), and enzymes such as proteases and lipase.

S. aureus is found in the vagina of approximately 16% of healthy women of menstrual age. Approximately 25% of the S. aureus isolated from the vagina are capable of producing TSST-1. TSST-1 and some of the staphylococcal enterotoxins have been identified as causing Toxic Shock Syndrome (TSS) in humans.

Symptoms of TSS generally include fever, diarrhea, vomiting and a rash followed by a rapid drop in blood pressure. Systemic vital organ failure occurs in approximately 6% of those who contact the disease. S. aureus does not initiate TSS as a result of the invasion of the microorganism into the vaginal cavity. Instead as S. aureus grows and multiplies, it can produce Toxic Shock Syndrome Toxin 1 (TSST-1 ; synonyms: pyrogenic exotoxin C and enterotoxin F). Only after entering the bloodstream does the TSST-1 toxin act systemically and produce the symptoms attributed to Toxic Shock Syndrome.

Menstrual fluid has a pH of approximately 7.3. During menses, the pH of the vagina moves toward neutral and can become slightly alkaline. This change permits microorganisms whose growth is inhibited by an acidic environment the opportunity to proliferate. For example, S. aureus is more frequently isolated from vaginal swabs during menstruation than from swabs collected between menstrual periods. There have been numerous attempts to reduce or eliminate pathogenic microorganisms and menstrually occurring TSS by incorporating into a tampon pledget one or more biostatic, biocidial, and/or detoxifying compounds. For example, L-ascorbic acid has been applied to a menstrual tampon to detoxify toxin found in the vagina of the human female during menstruation.

Incorporating glyceryl triacetate into a tampon pledget has been suggested. Glyceryl triacetate is readily broken down into glycerol and acetic acid by the enzymatic action of esterase. Esterase is present in the vaginal epithelium and in menstrual fluid. The enzymatic action of the esterase is in turn controlled by the pH of the environment, being more active when the pH is on the alkaline side. Since the pH of the vagina moves toward the alkaline side during menstruation, the enzymatic activity of the esterase automatically increases and attacks the glyceryl triacetate. This releases acetic acid rapidly, which has the potential to reduce the pH and enzymatic activity of the esterase. However, menstrual fluid is well buffered and the acetic acid is ineffective at lowering the pH of the menstrual fluid.

Others have incorporated monoesters and diesters of polyhydric aliphatic alcohols and a fatty acid containing from 8 to 18 carbon atoms. For example, glycerol monolaurate (GML) has been used to inhibit the production of S. aureus enterotoxins and TSST-1. However, as noted above, esterase is abundantly present in the vaginal epithelium and menstrual fluid. This esterase, in combination with esterase and lipase produced by bacteria can enzymatically degrade the esters into non-effective compounds.

Until now, persons skilled in the art have not appreciated the affects of lipase and esterase on ester compounds. Thus, one or more ester compounds may have to be added to the absorbent article, such as a tampon pledget, in sufficiently high concentrations to detrimentally effect the normal flora present in the vaginal area. When the natural condition is altered, overgrowth by pathogen(s) may take place resulting in a condition known as vaginitis.

Accordingly, there exists a need for an absorbent product that has incorporated therein a compound that will: effectively inhibit the production of exoproteins, such as TSST-1, from Gram positive bacterium; will be substantially unaffected by the enzymes lipase and esterase; and will not substantially alter the natural flora found in the vaginal area.

SUMMARY OF THE INVENTION Briefly, the present invention is based on the discovery that when an effective amount of one or more ether compounds or nitrogen containing compounds, such as amine and amide compositions, either alone or in combination, are incorporated into an absorbent article, such as a catamenial tampon, the production of exoprotein from Gram positive bacterium is substantially inhibited.

Ether compounds of the invention can be represented by the general formula: wherein ^ is a straight or branched chain alkyl group having from 8 to 18 carbon atoms and R ₂ is selected from an alcohol, a polyalkoxylated sulfate salt, or a polyalkoxylated sulfosuccinate salt. It has been observed that when these ether compound are incorporated into an absorbent article, such as a catamenial tampon, the production of exoprotein in Gram positive bacterium is substantially inhibited. Amine compounds of the invention can be represented by the general formula:

wherein R ₃ , is an alkyl group having from about 8 to about 18 carbon atoms; and R ₄ and R ₅ can be the same or different and are independently selected from hydrogen and alkyl group(s) having from 1 to about 18 carbon atoms. The alkyl groups of R ₄ and R ₅ can include one or more substitutional moieties selected from hydroxyl, carboxyl and carboxyl salts and imidazoline. It is also within the scope of this invention for the nitrogen containing compound to include an amine salt. It has been observed that amine compounds and salts thereof are effective in substantially inhibiting the production of exoprotein from Gram positive bacteria.

Amide compositions of the invention can be represented by the general formula:

O

1

Rτ-CN-R ₈ I

R ₉ wherein R ₇ , inclusive of the carbonyl carbon, is an alkyl group having 8 to 18 carbon atoms and R ₈ and R ₉ can be the same or different. Preferably, R ₈ and R ₉ are selected from hydrogen and an alkyl group having 1 to about 12 carbon atoms. The alkyl group of R ₈ and R ₉ may contain one or more substituent groups selected from ester, ether, amine, hydroxyl, carboxyl, carboxyl salts, sulfonate and sulfonate salts.

It is a general object of the invention to provide an absorbent article which inhibits the production of exoproteins from Gram positive bacterium. A more specific object of the invention is to provide a catamenial tampon incorporating one or more ether, amide or amine compounds, either alone or in combination which act to substantially inhibit the production of TSST-1 and Enterotoxin B by S. aureus. Another object of the invention is to provide a catamenial tampon that has incorporated therewith one or more ether, amine or amide compounds, either alone or in combination that will substantially inhibit the production of exoproteins from Gram positive bacterium without significantly imbalancing the natural flora present in the vaginal tract.

Another object of the invention is to provide a method for inhibiting the production exoprotein produced by Gram positive bacteria in an absorbent article. Other objects and advantages of the invention, and modifications thereof, will become apparent to persons skilled in the art without departure from the inventive concepts defined in the appended claims.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS This invention will be described in detail in connection with a catamenial tampon but would be understood by persons skilled in the art to be applicable to other disposable absorbent articles, such as: sanitary napkins, panty liners, adult incontinent undergarments, diapers, medical bandages and tampons such as those intended for medical, dental, surgical, and/or nasal use wherein inhibition of exoproteins from Gram positive bacteria would be beneficial.

Vaginal tampons suitable for use in this invention are usually made of absorbent fibers, including natural and synthetic fibers, compressed into a unitary body of a size which may easily be inserted into the vaginal cavity. They are normally made in an elongated cylindrical form in order that they may have a sufficiently large body of material to provide the required absorbing capacity, but can be made in a variety of shapes. The tampon may or may not be compressed, although compressed types are now generally preferred. The tampon can be made of various fiber blends including both absorbent and nonabsorbent fibers, which may or may not have a suitable cover or wrapper. It has been found that certain ether compounds can substantially inhibit the production of exoprotein of Gram positive bacterium, more specifically, the production of TSST-1 and Enterotoxin B from S. aureus bacterium. The ether compounds of the present invention have the general formula:

R O-R ₂ wherein Ri is a straight or branched alkyl group having a chain of 8 to 18 carbon atoms and R ₂ is selected from an alcohol, a polyalkoxylated sulfate salt or a polyalkoxylated sulfosuccinate salt.

The alkyl, or the Ri moiety of the ether compounds useful herein, can be derived from saturated and unsaturated fatty acid compounds. Suitable compounds include, C ₈ -C ₁₈ fatty acids, and preferably, the fatty acids include, without limitation, caprylic, capric, lauric, myristic, palmitic and stearic acid whose carbon chain lengths are 8, 10, 12, 14, 16 and 18, respectively. Highly preferred materials include capric, lauric, and myristic.

Preferred unsaturated fatty acids are those having one or two cis-type double bonds and mixtures of these materials. Suitable materials include myrystoleic, palmitoleic, linolenic and mixtures thereof.

Desirably, the R ₂ moiety is an aliphatic alcohol which can be ethoxylated or propoxylated for use in the ether compositions of the present invention. Suitable aliphatic alcohols include glycerol, sucrose, glucose sorbitol, sorbitan and derivatives thereof. Preferred ethoxylated and propoxylated alcohols include glycols such as ethylene glycol, propylene glycol, polyethylene glycol and polypropylene glycol.

The aliphatic alcohols can be ethoxylated or propoxylated by conventional ethoxylating or propoxylating compounds and techniques. The compounds are preferably selected from the group consisting of ethylene oxide, propylene oxide, and mixtures thereof, and similar ringed compounds which provide a material which is effective. Most preferably, the ethoxylation compound is selected from the group consisting of ethylene oxide, propylene oxide and mixtures thereof.

The R ₂ moiety can further include polyalkoxylated sulfate and polyalkoxylated sulfosuccinate salts. The salts can have one or more cations. Preferably, the cations are sodium, potassium or both. Preferred ether compounds of the present invention include laureth-3, laureth-

4, laureth-5, PPG-5 lauryl ether, 1-0-dodecyl-rac-glycerol, sodium laureth sulfate, potassium laureth sulfate, disodium laureth (3) sulfosuccinate, dipotassium laureth (3) sulfosuccinate and polyethylene oxide (2) sorbitol ether.

In accordance with this invention, the tampon contains an effective amount of the inhibiting ether compound to substantially inhibit the formation of TSST-1 when the tampon is exposed to S. aureus bacteria. Effective amounts have been found to be at least about 0.005 millimoles of ether compound per gram of absorbent. Preferably, the ether compound ranges from about 0.005 millimoles per gram of absorbent to about 2 millimoles per gram of absorbent and more preferably 0.005 millimoles per gram of absorbent to about 0.2 millimoles per gram of absorbent.

Although "compound" is used in the singular, one skilled in the art would understand that it includes the plural. That is, the absorbent article can include more than one ether compound.

In another embodiment of the invention certain nitrogen containing compounds can substantially inhibit the production of exoprotein of Gram positive bacteria, and more specifically, the production of TSST-1 and Enterotoxin B from S _^ aureus bacterium. Specifically, nitrogen containing compounds of the invention are amines and their salts having the general formula:

R ₃ -N-R ₄ |

R ₅ wherein R ₃ is an alkyl group having from about 8 to about 18 carbon atoms; and R ₄ and R can be the same or different and are selected from hydrogen and alkyl group(s) having from 1 to about 18 carbon atoms. The R ₄ and R ₅ alkyl groups can include one or more substitutional moieties selected from hydroxyl, carboxyl and

carboxyl salts and imidazoline. The amine compounds of the invention are effective in substantially inhibiting the production of exoprotein from Gram positive bacteria. It is critical to the invention that the R ₃ moiety be an alkyl group having from about 8 to about 18 carbon atoms. Desirably, the alkyl group is derived from fatty acid compounds which include, without limitation, caprylic, capric, lauric, myristic, palmitic and stearic acid whose carbon chain lengths are 8, 10, 12, 14, 16 and 18, respectively. Highly preferred materials include capric, lauric, and myristic. Preferred unsaturated fatty acids are those having one or two cis-type double bonds and mixtures of these materials. Suitable materials include myrystoieic, palmitoleic, linolenic and mixtures thereof.

The R ₄ and R ₅ alkyl groups can further include one or more substitutional moieties selected from hydroxyl, carboxyl, carboxyl salts, and R ₃ and R ₄ can form an unsaturated heterocyclic ring that contains a nitrogen that connects via a double bond to the alpha carbon of the R ₅ moiety to form a substituted imidazoline. The carboxyl salts can have one or more cations selected from sodium, potassium or both. The R ₃ -R _s alkyl groups can be straight or branched and can be saturated or unsaturated.

In another embodiment, the amine compound can be a salt. The salt can be represented by the general formula: R ₆

I R ₃ ⁺ -N-R ₄

I R ₅ wherein R ₃ is an anionic moiety associated with the amine and is derived from an alkyl group having from about 8 to about 18 carbon atoms. Desirably, R ₃ is a polyalkyloxylated alkyl sulfate. The R ₆ moiety is the same as that described above for the R ₄ and R ₅ moieties. The R ₃ -R ₆ alkyl groups can be straight or branched and can be saturated or unsaturated. A preferred compound illustrative of an amine salt is TEA laureth sulfate.

Preferred amine compounds of the present invention which may be incorporated into the absorbent article or onto the cover thereof include: TEA laureth sulfate, iauramine, lauramino propionic acid, sodium lauriminodipropionic acid, lauryl hydroxyethyl imidazoline and mixtures thereof.

In accordance with the invention, the tampon contains an effective amount of the inhibiting nitrogen containing compound to substantially inhibit the formation of TSST-1 when the tampon is exposed to S. aureus bacteria. Effective amounts have been found to be at least about 1 x (10 ⁵ ) millimoles of the amine compound per gram of absorbent. Preferably, the amine compound ranges from about 0.005 millimoles per gram of absorbent to about 2 millimoles per gram of absorbent and more preferably 0.005 millimoles per gram of absorbent to about 0.2 millimoles per gram of absorbent. Although "compound" is used in the singular, one skilled in the art would understand that it includes the plural. That is, the absorbent article can include more than one amine compound.

In another embodiment of the invention, the nitrogen containing compounds of the invention have the general formula:

O

I R _r CN-R ₈ wherein R ₇ , inclusive of the carbonyl carbon, is an alkyl group having 8 to 18 carbon atoms and R ₈ and R ₉ can be the same or different. R ₈ and R ₉ are selected from hydrogen and an alkyl group having 1 to about 12 carbon atoms which may contain one or more substituent groups selected from ester, ether, amine, hydroxyl, carboxyl, carboxyl salts, sulfonate and sulfonate salts.

The alkyl moiety, which includes the carbonyl carbon, can be derived from saturated and unsaturated fatty acid compounds. Suitable compounds include, C ₈ - Cιβ fatty acids, and preferably, the fatty acids include, without limitation, caprylic, capric, lauric, myristic, palmitic and stearic acid whose carbon chain lengths are 8,

10, 12, 14, 16 and 18, respectively. Highly preferred materials include capric, lauric, and myristic.

Preferred unsaturated fatty acids are those having one or two cis-type double bonds and mixtures of these materials. Suitable materials include myrystoieic, palmitoleic, linolenic and mixtures thereof.

The R ₈ and R ₉ moieties can be the same or different and each being selected from hydrogen and an alkyl group having a carbon chain having from 1 to about 12 carbon atoms. The R ₇ -R ₉ alkyl groups can be straight or branched and can be saturated or unsaturated. When R ₈ and/or R ₉ are an alkyl moiety having a carbon chain of at least 2 carbons, the alkyl group can include one or more substituent groups selected from ester, ether, amine, hydroxyl, carboxyl, carboxyl salts,

sulfonate and sulfonate salts. The salts can have one or more cations selected from sodium, potassium or both.

Preferred amide compounds of the present invention include sodium lauroyl sarcosinate, lauramide MEA, lauramide DEA, lauramidopropyl dimethylamine, disodium lauramido MEA sulfosuccinate and disodium lauroamphodiacetate.

In accordance with the invention, the tampon contains an effective amount of the inhibiting nitrogen containing compound to substantially inhibit the formation of TSST-1 when the tampon is exposed to S. aureus bacteria. Effective amounts have been found to be at least about 5 x (10 ^" ) millimoles of the amide compound per gram of absorbent. Preferably, the amide compound ranges from about 0.005 millimoles per gram of absorbent to about 2 millimoles per gram of absorbent. More preferably the amide compound ranges from about 0.005 millimoles per gram of absorbent to about 0.2 millimoles per gram of absorbent. Although "compound" is used in the singular, one skilled in the art would understand that it includes the plural. That is, the absorbent article can include more than one amide compound. The compositions of the present invention can be prepared and applied in any suitable form, but are preferably prepared in forms including, without limitation aqueous solutions, lotions, balms, gels, salves, ointments, boluses, suppositories, and the like. The compositions may be applied to the absorbent article using conventional methods for applying an inhibitory agent to the desired absorbent article. For example, unitary tampons without separate wrappers, may be dipped directly into a liquid bath having the agent and then can be air dried, if necessary to remove any volatile solvents. For compressed tampons, impregnating of any of its elements is best done before compressing. The compositions when incorporated on and/or into the tampon materials may be fugitive, loosely adhered, bound, or any combination thereof. As used herein the term "fugitive" means that the composition is capable of migrating through the tampon materials.

It is not necessary to impregnate the entire absorbent body of the tampon with the inhibitory agent. Optimum results both economically and functionally, can be obtained by concentrating the material on or near the outer surface where it will be most effective during use.

The substantially inhibitory composition may additionally employ one or more conventional pharmaceutically-acceptable and compatible carrier materials useful for the desired application. The carrier can be capable of co-dissolving or suspending the materials used in the composition. Carrier materials suitable for use in the instant composition, therefore, include those well-known for use in the cosmetic and medical arts as a basis for ointments, lotions, creams, salves, aerosols, suppositories, gels and the like.

The inhibitory compositions of the present invention may additionally employ adjunct components conventionally found in pharmaceutical compositions in their art-established fashion and at their art-established levels. For example, the compositions may contain additional compatible pharmaceutically active materials for combination therapy, such as supplementary antimicrobials, anti-parasitic agents, antipruritics, astringents, local anesthetics, or anti-inflammatory agents. The present invention may be readily understood by considering the following examples illustrative of specific embodiments. The Examples are given to serve as a guide in carrying out the invention and are not to be construed as a limitation or limitations of the invention. It will be understood that various changes or modifications may be made, as will be apparent to those skilled in the art, without departing from the spirit of the invention or the scope of the claims annexed hereto.

EXAMPLES 1-3 The efficacy of the test compounds on TSST-1 production was determined by placing the desired concentration, expressed in millimoles/milliiiter (millimoiar hereinafter mM) of the active compound in 10 milliliters of a Growth Medium of each test compound in a Corning 50 ml conical polystyrene tube. The polystyrene tube is available from Scientific Products Division, Baxter Diagnostics Incorporated, 1430 Waukegan Road, McGaw Park, IL 60085-6787.

The Growth Medium was prepared as follows: Brain heart infusion broth (BHI), available from Becton Dickinson Microbiology Systems, Cockeysville, MD 21030, was dissolved and sterilized according to the manufacturer's instructions. Ninety milliliters of BHI broth was supplemented with 10 ml fetal bovine serum (FBS), available from Sigma Chemical Company, P.O. Box 14508, St. Louis, MO 63178- 9916. One milliliter of a 0.02 molar sterile solution of the hexahydrate of magnesium chloride, available from Sigma Chemical Company, was added to the

BHI-FBS mixture. One milliliter of a 0.027 molar sterile solution of L-glutamine available from Sigma Chemical Company was also added to the BHI-FBS mixture.

If the test compound was not water soluble or water miscible, it was first dissolved at 50 times the desired concentration in 10 ml isopropanol, then diluted to the desired final concentration in 10 ml of the Growth Medium. Tubes of Growth Medium with an equivalent amount of isopropanol, but no test compound, were prepared as controls.

In preparation for inoculation of the tubes of Growth Medium containing the test compound, an inoculating broth was prepared as follows. S. aureus. (MN8) was streaked onto a sheep blood agar plate and incubated at 37°C. The test organism in this Example was obtained from Dr. Pat Schlievert, Department of Microbiology of the University of Minnesota Medical School, Minneapolis, MN. After 24 hours of incubation three to five individual colonies were picked with a sterile inoculating loop and used to inoculate 10 ml of the Growth Medium. The tube of inoculated Growth Medium was capped with a S/P® diSPo® plug available from Scientific Products Division, Baxter Diagnostics, Incorporated and incubated at 37°C in atmospheric air having 5% by volume CO ₂ . After 24 hours of incubation, the culture was removed from the incubator and mixed well on a S/P brand vortex mixer. A second tube containing 10 ml of the Growth Medium was inoculated with 0.5 ml of the above 24 hour culture and re-incubated at 37°C in atmospheric air having 5% by volume CO ₂ . After 24 hours of incubation the culture was removed from the incubator and mixed well on a S/P brand vortex mixer. Each tube of Growth Medium containing a test compound and growth control tubes with or without isopropanol were inoculated with 0.1 ml of the prepared inoculating broth. The initial colony forming units (CFU) per ml of Growth Medium were approximately 1 x 10 ⁷ . The tubes were capped with S/P® diSPo® plugs and incubated at 37°C in atmospheric air having 5% by volume CO ₂ . After 24 hours of incubation the tubes were removed from the incubator and the culture fluid was assayed for the number of colony forming units of S. aureus and prepared for analysis of TSST-1 per method described below.

The number of colony forming units per ml after incubation was determined by standard plate count procedure. The culture fluid broth was centrifuged and the supernatant subsequently filter sterilized through an Acrodisc® syringe filter unit available from Scientific Products Division, Baxter Diagnostics, Inc. The resulting fluid was frozen at -80°C until assayed.

The amount of TSST-1 per milliliter was determined by a non-competitive, sandwich enzyme-linked immunoabsorbent assay (ELISA). Samples of the culture fluid and the TSST-1 reference standard were assayed in triplicate. The method employed was as follows: Four reagents, rabbit polyclonal anti-TSST-1 IgG (#LTI- 101), rabbit polyclonal anti-TSST-1 IgG conjugated to horseradish peroxidase

(#LTC-101), TSST-1 (#TT-606), and normal rabbit serum certified anti-TSST-1 free (#NRS-10) were purchased from Toxin Technology, Incorporated, 7165 Curtiss Avenue, Sarasota, Florida, 34231. Sixty-two microliters of polyclonal rabbit anti-TSST-1 IgG (#LTI-101) was appropriately diluted so that a 1:100 dilution gave an absorbency of 0.4 at 650 nanometers. This was added to 6.5 ml of 0.5 molar carbonate buffer, pH 9.6, and 100 microliters of this solution was pipetted into the inner wells of polystyrene microtiter plates #439454, obtained from Nunc-Denmark. The plates were covered and incubated overnight at 37°C. Unbound antitoxin was removed by three washes with phosphate buffered saline (pH 7.2) (0.011 molar NaH ₂ PO and 0.9% [wt/vol] NaCI both available from Sigma Chemical Company) containing 0.5% [vol/vol] Tween 20 (PBS-Tween), also available from Sigma Chemical Company. The plates were treated with 100 microliters of a 1% [wt/vol] solution of bovine serum albumin (BSA), available from Sigma Chemical Company, covered, and incubated at 37°C for one hour. Unbound BSA was removed by 6 washes with PBS-Tween. TSST-1 reference standard, serially diluted from 1 - 10 ng/ml in PBS-Tween, test samples treated with normal rabbit serum 10% [vol/vol] final concentration and reagent controls were pipetted in 100 microliter volumes to their respective wells. This was followed by incubation for two hours at 37°C and three washes to remove unbound toxin. The rabbit polyclonal anti-TSST-1 IgG conjugated to horseradish peroxidase and diluted according to manufacturer's instructions was added (100 microliter volumes) to each microtiter well. The plates were covered and incubated at 37°C for one hour.

Following incubation the plates were washed 6 times in PBS-Tween. Following this, the wells were treated with a solution consisting of 0.075 molar sodium citrate (pH 4.0), 0.6 millimolar 2,2'-Azino-bis-(3-ethylbenzthiazoline-6- sulfonic acid) diammonium salt and 0.009% [vol/vol] hydrogen peroxide, all available from Sigma Chemical Company. The intensity of the color reaction in each well was evaluated over time using a BioTek Model EL340 Microplate reader (OD 405 nm) and Kineticalc® software available from Biotek Instruments, Inc. TSST-1 concentrations in test samples were predicted from the reference toxin regression equations derived during each assay procedure. The efficacy of the ether, amine and amide compounds in inhibiting the production of TSST-1 is shown below in Tables I— I II, respectively.

TABLE ! mM Test ELISA: TSST-1

Compound Compound CFU/ml ng/ml

Growth control None 3.3 X 10 ⁹ 381.8

1 -0-dodecyl-rac-glycerol 10.67 1.2 X 10 ⁹ 19.8

Laureth-3 9.03 2.4 X 10 ⁸ 3.7

Laureth-4 10.20 2.4 X 10 ⁸ 2.3

Sodium laureth sulfate 10.65 2.9 X 10 ³ ND

PPG-5 Laureth-5 7.35 2.0 X 10 ⁸ 1.6

Disodium laureth sulfosuccinate 9.84 3.4 X 10 ⁸ 2.3 ND = None detected

The above list of compounds (Commercial Name), their percent active compound, and vendor are as follows: 1-0-dodecyl-rac-glycerol, Sigma Chemical Company, 100%, P.O. Box 14508,

St. Louis, MO 63178-9916.

Laureth-3, (Trycol 5966), 97%, Henkle Corporation, Emery Group, 4900 Este Avenue, Cincinnati, Ohio, 45232.

Laureth-4, (Trycol 5882), 100%, Henkle Corporation, 300 Brookside Avenue, Ambler, Pennsylvania 19002.

Sodium laureth sulfate, (Standapol ES-2), 25%, Henkle Corporation. PPG-5 Laureth-5, (Aethoxal B), 100%, Henkle Corporation.

Disodium laureth sulfosuccinate, (Standapul SH124-3), 39%, Henkle Corporation.

In accordance with the present invention, the data in Table I shows that & aureus MN8, when compared to the control, produced significantly less TSST-1 in the presence of the ether compounds. The ether compounds reduced the amount of exotoxin production ranging from about 95 percent to greater than 99.6 percent. However, although the amount of toxin produced was significantly reduced, in most cases the ether compounds did not substantially reduce the number of S. aureus cells.

TABLE II mM Test ELIS >A: TSST-1

Compound Compound CFU/ml nq/ml Growth Control None 2.5 x 10 ⁹ 153.2 Laurimino propionic acid 10.67 No Growth <1.1 0.43 Not Determined* 24.2

Sodium lauriminodipropionic 10.67 1.0 x 10 ³ <1.1 acid 2.15 Not Determined 2.9

Lauryl hydroxyethyl 10.67 No Growth <1.1 imidazoline 0.43 Not Determined 85.9 TEA laureth sulfate 10.67 6.2 x 10 ² <1.1 2.15 None Detected 4.7

Lauramine 10.67 No Growth <1.1 2.15 Not Determined 77.0

* S. aureus grew in the culture broth, the amount of growth was not determined.

The above list of compounds (Commercial Name), their percent active compound, and vendor are as follows:

Laurimino propionic acid, (Mackam 151L), 40%, Mclntyre Group, LTD, 1000 Governors Highway, University Park, IL. 60466.

Sodium lauriminodipropionic acid, (Deriphat 160-C), 30%, Henkle/Cospha, Cospha Group, 300 Brookside Ave., Ambler, PA 19002.

Lauryl hydroxyethyl imidazoline, (Schercozoline L), Scher Chemicals, Inc., Industrial West, P.O. Box 4317, Clifton, NJ 07012.

TEA laureth sulfate, (Sulfochem TLES), 39%, Chemron, P O Box 2299, Paso-Robles, CA 93447.

Lauramine.(Dodecylamine), 99+, Aldrich, 1001 West Saint Paul Ave, Milwaukee, WI 53233.

In accordance with the present invention, the data in Table II shows that S. aureus MN8, when compared to the control, produced significantly less TSST-1 in the presence of the amine compounds. The amine compounds reduced the amount of exotoxin production ranging from about 86 percent to greater than 99.4 percent.

TABLE mM Test ELISA: TSST-1

Compound Compound CFU/ml ng/ml

Growth Control None 3.1 x 10 ⁹ 381.8

Lauramide MEA 10.67 1.5 x 10 ⁹ 51.6

Sodium lauroyl sarcosinate 10.70 1.4 x 10 ³ <1.1

Disodium lauroamphodiacetate 10.74 3.5 x 10 ⁸ 2.9

Disodium lauramido MEA sulfosuccinate 10.71 9.1 x 10 ⁸ 1.2

The above list of compounds (Commercial Name), their percent active compound, and vendor are as follows:

Lauramide MEA, (Comperlan LNN), 98.5%, Henkle Corporation, 300 Brookside Avenue, Ambler, Pennsylvania 19002.

Sodium lauroyl sarcosinate, (Hamposyl L-30), 30%, Hampshire Chemical Co. 55 Hayden Ave., Lexington, MA 02173.

Disodium lauroamphodiacetate, (Mackam 2-L), 50% Mclntyre Group, 1000 Governors Highway, University Park, IL. 60466.

Disodium lauramido MEA sulfosuccinate, (Mackanate LM-40), 40%, Mclntyre Group, 1000 Governors Highway, University Park, IL. 60466. In accordance with the present invention, the data in Table III shows that S^ aureus MN8, when compared to the control, produced significantly less TSST-1 in the presence of the amide compounds. The amide compounds reduced the amount of exotoxin production ranging from about 86 percent to greater than 99.6 percent.

EXAMPLES 4-6 The efficacy of the test compounds in reducing the production of a second exoprotein of S. aureus was determined using S. aureus HOCH, a known producer of Enterotoxin B. The test organism in this Example was obtained from Dr. Pat Schlievert, Department of Microbiology of the University of Minnesota Medical

School, Minneapolis, MN. The experimental procedure for assessing the efficacy of the test compounds effect on growth of S. aureus HOCH and for production of a culture filtrate was the same as set forth in Example A above.

The amount of S. aureus enterotoxin B per milliliter was determined by Western Blot assay. Samples of the culture fluid and the Staphylococcal

Enterotoxin B (SEB) reference standard were assayed in triplicate. The method employed was similar to that described in "A Rapid, Sensitive Method for Detection of Alkaline Phosphatase-Conjugated Anti-Antibody on Western Blots," M.S. Blake, K.H. Johnston, G.J. Russell-Jones and E. C. Gotschlich, Analytical Biochemistry, 136:175-179, 1984. The disclosure of which is incorporated herein by reference. Enterotoxin B was separated from other proteins in the test samples by sodium dodecyl sulfate-polyacrylamide gel (SDS-PAGE) electrophoresis. The upper stacking gel used 3% acrylamide gel, the lower separating gel contained 14% acrylamide gel. The upper gel was prepared with a comb containing twenty lanes spanning 15.5 centimeters of the top of the stacking gel. A low molecular weight SDS-PAGE standard, BioRad #161-0305 available from Bio-Rad Laboratories having offices at 2000 Alfred Nobel Drive, Hercules, CA 94547, SEB, #BT-202 from Toxin Technology, Incorporated, and culture extracts, as prepared above, were each mixed 1:1 with a loading buffer. The loading buffer contained 30% [vol/vol] glycerol, 15% [vol/vol] mercaptoethanol, 7% [wt/vol] sodium dodecyl sulfate,

0.0036% [wt/vol] bromphenol blue, and 0.76% [wt/vol] Trizma base with a pH of 6.8. The mixtures were boiled for 5 minutes, then twenty-five (25) microliters of each mixture was placed in a lane. The SDS-PAGE gel was electrophoresed (60 to 80 volts) for 90 minutes or until the dye front was through the stacking gel and for an additional 2.5 hours (160-170 volts) with an electrophoresis buffer of 0.61% [wt/vol] Tris base, 2.85% [wt/vol] glycine, 0.1% [wt/vol] SDS, pH 7.85. Proteins were transferred to a nitrocellulose transfer membrane, available from Schleicher and Schull, Inc., Keene, N.H. 03431, #BA 85, overnight approximately 15 hours at 200 milliamps in a Bio-Rad Trans-Blot® cell. The transfer buffer was composed of 0.3% [wt vol] Tris base, 1.4% [wt/vol] glycine, 20% [vol/vol] methanol, pH 7.6.

The nitrocellulose membrane was treated for 45 minutes at 37°C with 3% [wt/vol] gelatin in 0.02 molar Tris buffer, 0.5 molar NaCI, at pH 7.5 (TBS) to block non-specific reactions, then washed at 37°C with TBS-0.05% [vol/vol] Tween 20 (TBS-Tween) for 45 minutes. The membrane was then submerged for 1.5 hours at 37°C in 50 ml TBS-Tween containing 0.05 ml rabbit polyclonal anti-SEB IgG, #LBI-202 available from Toxin Technology, Incorporated.

The membrane was washed two times in TBS-Tween, then submerged a second time for 1.5 hours at 37°C in a 50 ml solution of TBS-Tween with 25 microliters of goat anti-rabbit IgG conjugated to alkaline phosphatase. The membrane was washed twice in TBS-Tween and twice in TBS. The blot was developed with a reaction solution consisting of 2 mg 5-bromo-4-chloroindolyl phosphatase, 100 microliters of N,N dimethyl formamide, 18 ml 0.15 molar barbitol buffer, pH 9.2, 2 mg nitroblue tetrazolium, and 40 microliters of a 2 molar solution of MgCI ₂ -6H ₂ O, all available from Sigma. The reaction was stopped with a cold water wash. The amount of SEB produced in the presence of the test compound was estimated by a comparison with the staining intensity produced by a serial dilution of SEB.

The efficacy of the ether, amine and amide compounds in inhibiting the production of Enterotoxin B is shown below in Tables IV-VI, respectively.

TABLE IV mM Test Western Blot: SEB

Compound Compound CFU/ml mq/ml

Growth control None 1.0 X 10 ⁹ 0.8

1 -0-dodecyl-rac-glycerol 10.67 7.0 X 10 ⁸ 0.8

Laureth-3 9.03 8.3 X 10 ⁸ ND

Laureth-4 10.20 7.5 X 10 ⁸ ND

Sodium laureth sulfate 2.13 1.2 X 10 ⁷ ND

PPG-5 Laureth-5 7.35 6.4 X 10 ⁸ ND

Disodium laureth sulfosuccinate 9.84 2.1 X 10 ⁷ ND

ND = None detected, <0.16 mg/ml

In accordance with the present invention, the data in Table IV shows that S aureus HOCH, when compared to the control, produced less SEB in the presence of the ether compounds. However, although the amount of toxin produced was substantially reduced, the ether compounds did not significantly reduce the number of S. aureus cells.

TABLE V mM Test Western Blot: SEB

Compound Compound CFU/ml mo/ml Growth control None 7.0 x 10 ⁹ 0.8

Laurimino 10.67 No growth None detected propionic acid 0.43 Not determined* None detected

Sodium 10.67 1.3 x 10 ³ None detected lauriminodipropionic acid 2.15 Not determined None detected

Lauryl hydroxyethyl 10.67 No growth None detected imidazoline 0.43 Not determined None detected

TEA laureth 10.67 5.6 x 10 ² None detected sulfate 2.15 Not determined None detected Lauramine 10.67 No growth None Detected 2.15 Not determined 0.2

* S. aureus grew in the culture broth, the amount of growth was not determined. In accordance with the present invention, the data in Table II shows that aureus HOCH produced significantly less SEB in the presence of the amine compounds. Compared to the control, the amine compounds reduced the amount of exotoxin production below the detectable range of 0.16 milligrams/milliliter.

TABLE IV

mM Test Western Blot: SEB

Compound Compound CFU/ml mg/ml

Growth Control None 1.5 x 10 ⁹ 0.8

Lauramide MEA 10.67 3.7 x 10 ,8' None Detected

Sodium lauroyl sarcosinate 10.70* 1.7 x 10 ^J None Detected

Disodium lauroamphodiacetate 10.74 1.2 x 10 ⁸ None Detected

Disodium lauramido MEA sulfosuccinate 10.71 1.2 x 10 ,8 None Detected

* No SEB detected at 0.43 millimole concentration of sodium lauroyl sarcosinate, no plate count was performed.

None Detected = <0.16 mg/ml.

In accordance with the present invention, the data in Table II shows that S _^ aureus HOCH, when compared to the control, produced significantly less SEB in the presence of the amide compounds. However, although the amount of toxin produced was reduced below detectable level, the concentration of the amide compound can be adjusted so that there was little reduction in the number of S. aureus cells. The amide compounds reduced the amount of exotoxin production below the detectable range of 0.16 milligrams/milliliter.

Another aspect of the invention is for a method of inhibiting the production of exoprotein from Gram positive bacteria in an absorbent product. The method includes the steps of contacting the absorbent article, such as a tampon, with one or more of the above described inhibitory compositions then placing the absorbent article for use so that the production of exoprotein from Gram positive bacteria is inhibited. The absorbent article can have one or more of the inhibitory compositions absorbed into or coated onto the fibers or cover material. Desirably, the production of TSST-1 and Enterotoxin B are inhibited.

While the invention has been described in conjunction with a specific embodiment, it is to be understood that many alternatives, modifications and variations will be apparent to those skilled in the art in light of foregoing description. Accordingly, this invention is intended to embrace all such alternatives, modifications and variations which fall within the spirit and scope of the appended claims.