Title:
INHIBITOR OF NON-SPECIFIC BINDING OF NUCLEIC ACID, HYBRIDIZATION REAGENT AND NUCLEIC ACID HYBRIDIZATION METHOD
Document Type and Number:
WIPO Patent Application WO/2020/090822
Kind Code:
A1
Abstract:
Disclosed are: a non-specific binding inhibitor, which efficiently inhibits cross hybridization between a target nucleic acid and a nucleic acid having a sequence similar to the complementary chain thereof in the case of detecting the target nucleic acid by hybridization and the qualities of which are consistent from production lot to production lot; and a nucleic acid hybridization method using the non-specific binding inhibitor. The inhibitor of non-specific binding of nucleic acid comprises a nucleic acid that has a base length of 2-11 bases and shows a guanine or methylated guanine content of 70% or greater relative to the total base sequence.
Inventors:
SERIZAWA TAKASHI (JP)
Application Number:
PCT/JP2019/042394
Publication Date:
May 07, 2020
Filing Date:
October 29, 2019
Export Citation:
Assignee:
TORAY INDUSTRIES (JP)
International Classes:
C12N15/11; C12Q1/6832; C12Q1/6876
Foreign References:
| JP2010029174A | 2010-02-12 |
Other References:
MATVEEVA, 0. V. ET AL.: "Optimization of signal- to-noise ratio for efficient microarray probe design", BIOINFORMATICS, vol. 32, 2016, pages i552 - i558, XP055704480
"Implementation of DNA Microarray Hybridization Protocols, Translation of Manual Methods to Tecan Hybridization Station", 22 October 2018, TECAN JAPAN CO., LTD.
PAN SJ ET AL., BMC GENOMICS, vol. 3, 2002, pages 1 - 12
KU WC. ET AL., BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 315, 2004, pages 30 - 37
SAMBROOK, J. ET AL.: "Molecular Cloning: A Laboratory Manual", 1998, COLD SPRING HARBOR LABORATORY PRESS
See also references of EP 3875586A4
"Implementation of DNA Microarray Hybridization Protocols, Translation of Manual Methods to Tecan Hybridization Station", 22 October 2018, TECAN JAPAN CO., LTD.
PAN SJ ET AL., BMC GENOMICS, vol. 3, 2002, pages 1 - 12
KU WC. ET AL., BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 315, 2004, pages 30 - 37
SAMBROOK, J. ET AL.: "Molecular Cloning: A Laboratory Manual", 1998, COLD SPRING HARBOR LABORATORY PRESS
See also references of EP 3875586A4
Attorney, Agent or Firm:
TANIGAWA AND PARTNERS, PATENT FIRM (JP)
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