PI3Ka INHIBITORS

TECHNICAL FIELD

The present disclosure provides compounds as well as their pharmaceutical compositions that modulate the activity of PI3Ka and are useful in the treatment of various diseases related to PI3Ka, including cancer.

BACKGROUND

In the past few decades, signal transduction events have been studied to demonstrate critical roles in regulating almost all aspects of biological responses. Aberrant activation of the signaling pathways regulating cell survival and proliferation is commonly observed in many human cancers. The phosphoinositide 3-kinases (PI3Ks) signaling pathway is documented to be one of the highly mutated pathways in human cancers (Vogelstein et al., Science, 2013, 339(6127), 1546-1558). The PI3K signaling pathway regulates cell survival and proliferation. Increased activity of this pathway is associated with tumor progression and resistance to cancer therapies (Fusco et al., Front Oncol., 2021, 11, 644737).

PI3Ks belong to a lipid kinase family which catalyzes the phosphorylation of lipids contained in or associated with cell membranes. The PI3K family has fifteen kinases with distinct substrates, expression pattern, and modes of regulation. The class-I PI3Ks (pl 10a, pl 10p, pl 105, and pl 10y) are typically activated by tyrosine receptor kinases or G-protein coupled receptors to generate PIP3, which activates downstream effectors of Akt, mTOR, or Rho GTPases (Fruman et al., Nat. Rev. Drug Discov., 2014, 13(2), 140-156).

Genetic mutations in the gene coding for PI3Ka are hotspot point mutations within helical and kinase domains, such as E542K, E545K and H1047R. These mutations have been observed to occur in many cancer types such as lung, stomach, endometrial, ovarian, bladder, breast, colon, brain, prostate, and skin cancers. Because these gain-of-function mutations in PI3Ka are associated with tumor progression, targeting this pathway may provide valuable therapeutic opportunities (Courtney et al., J. Clin. Oncol., 2010, 28 (6), 1075-1083). While multiple inhibitors of PI3Ks have been developed (for example, taselisib, alpelisib, buparlisib and others), these molecules inhibit multiple PI3K isoforms. These “pan-PI3K” inhibitors have encountered major hurdle in the clinical development due to inability to achieve the required level of target inhibition in tumors while avoiding toxicity in cancer patients (Fruman et al., Nat. Rev. Drug Discov., 2014, 13(2), 140-156). The toxicity of PI3K inhibitors is dependent on their isoform selectivity profde. Inhibition of PI3Ka is associated with hyperglycemia and rash, while inhibition of PI3K5 or PI3Ky is associated with diarrhea, myelosuppression, and transaminitis (Hanker et al., Cancer Discov., 2019, 9(4), 482-491). Therefore, selective inhibitors of PI3Ka may increase the therapeutic window, enabling sufficient target inhibition in the tumor while avoiding dose -limiting toxicity in cancer patients. However, given the central role of PI3Ka in regulating glucose homeostasis and other critical physiological process, current PI3Ka selective inhibitors, which are equally potent to wild-type and mutant PI3Ka, often cause hyperglycemia and/or hyperinsulinemia (Busaidy et al., J. Clin. Oncol., 2012, 30, 2919-2928). In summary, developing inhibitors with enhanced selectivity for mutant PI3Ka against wild-type PI3Ka would be able to overcome the problem of compensatory insulin production and hyperglycemia.

SUMMARY

The present disclosure provides compounds and/or compositions useful for inhibiting PI3Ka. In some embodiments, provided compounds and/or compositions are useful for selectively inhibiting PI3Ka over other PI3K isoforms. In some embodiments, provided compounds and/or compositions are useful for selectively inhibiting mutant PI3Ka over wide- type PI3Ka. In some embodiments, provided compounds and/or compositions are useful, among other things, treating and/or preventing diseases, disorders, or conditions associated with PI3Ka. In some embodiments, provided compounds and/or compositions are useful, among other things, treating and/or preventing diseases, disorders, or conditions associated with mutant PI3Ka.

In some embodiments, the present disclosure provides certain compounds and/or compositions that are useful in medicine, and particularly for treating cancer.

In some embodiments, the present disclosure provides a compound of Formula I:

I or a pharmaceutically acceptable salt thereof, wherein each of X, Y, Z, U, Ring A, L ^A , R ^A , R ¹ , R ³ , R ⁵ , and n is as defined herein.

In some embodiments, provided compounds have structures of any of Formulae II, III, IV, V, Va, VI, Via, VII, Vila, and Vllb as described herein.

In some embodiments, provided compounds have structures of any of Formulae II, III, IV, V, Va, VI, and Via as described herein. In some embodiments, the present disclosure provides compositions that comprise and/or deliver a provided compound. In some embodiments, such compositions are pharmaceutical compositions comprising a pharmaceutically acceptable carrier.

The present disclosure further provides methods of inhibiting PI3Ka activity, comprising contacting the PI3Ka with a compound described herein, or a pharmaceutically acceptable salt thereof.

The present disclosure further provides methods of treating a disease or a disorder associated with PI3Ka in a patient by administering to the patient a therapeutically effective amount of a compound of the disclosure, or a pharmaceutically acceptable salt thereof.

The present disclosure further provides a compound described herein, or a pharmaceutically acceptable salt thereof, for use in any of the methods described herein.

The present disclosure further provides use of a compound described herein, or a pharmaceutically acceptable salt thereof, for the preparation of a medicament for use in any of the methods described herein.

DETAILED DESCRIPTION

Compounds and Definitions

Compounds of this invention include those described generally above, and are further illustrated by the classes, subclasses, and species disclosed herein. As used herein, the following definitions shall apply unless otherwise indicated. For purposes of this invention, the chemical elements are identified in accordance with the Periodic Table of the Elements, CAS version, Handbook of Chemistry and Physics, 75 ^th Ed. Additionally, general principles of organic chemistry are described in “Organic Chemistry”, Thomas Sorrell, University Science Books, Sausalito: 1999, and “March’s Advanced Organic Chemistry”, 5 ^th Ed., Ed.: Smith, M.B. and March, J., John Wiley & Sons, New York: 2001, the entire contents of which are hereby incorporated by reference.

Unless otherwise stated, structures depicted herein are meant to include all stereoisomeric (e.g., enantiomeric or diastereomeric) forms of the structure, as well as all geometric or conformational isomeric forms of the structure. For example, the R and S configurations of each stereocenter are contemplated as part of the disclosure. Therefore, single stereochemical isomers, as well as enantiomeric, diastereomic, and geometric (or conformational) mixtures of provided compounds are within the scope of the disclosure. For example, in some case, Table 1 shows one or more stereoisomers of a compound, and unless otherwise indicated, represents each stereoisomer alone and/or as a mixture. Unless otherwise stated, all tautomeric forms of provided compounds are within the scope of the disclosure. Unless otherwise indicated, structures depicted herein are meant to include compounds that differ only in the presence of one or more isotopically enriched atoms. The isotopically-labeled compounds may have one or more atoms replaced by an atom having an atomic mass or mass number usually found in nature. Examples of isotopes present in compounds of the present disclosure include isotopes of hydrogen, carbon, nitrogen, oxygen, phosphorus, fluorine and chlorine, such as, but not limited to, ² H, ³ H, ¹³ C, ¹⁴ C, ¹⁵ N, ¹⁷ O, ¹⁸ O, ³⁵ S and ¹⁸ F. Certain isotopically-labeled compounds of the present disclosure, in addition to being useful as therapeutic agents, are also useful in drug and/or substrate tissue distribution assays, as analytical tools or as probes in other biological assays. In one aspect of the present disclosure, tritiated (e.g., ³ H) and carbon-14 (e.g., ¹⁴ C) isotopes are useful given their ease of detectability. In another aspect of the present invention, replacement of one or more hydrogen atoms with heavier isotopes such as deuterium, (e.g., ² H) can afford certain therapeutic advantages.

As used herein and unless otherwise specified, the suffix “-ene” is used to describe a bivalent group. Thus, any of the terms above can be modified with the suffix “-ene” to describe a bivalent version of that moiety. For example, a bivalent carbocycle is “carbocyclylene”, a bivalent aryl ring is “arylene”, a bivalent benzene ring is “phenylene”, a bivalent heterocycle is “heterocyclylene”, a bivalent heteroaryl ring is “heteroarylene”, a bivalent alkyl chain is “alkylene”, a bivalent alkenyl chain is “alkenylene”, a bivalent alkynyl chain is “alkynylene”, and so forth.

Aliphatic: As used herein, the term “aliphatic” refers to a straight-chain (i.e., unbranched) or branched, optionally substituted hydrocarbon chain that is completely saturated or that contains one or more units of unsaturation, or a monocyclic or bicyclic hydrocarbon that is completely saturated or that contains one or more units of unsaturation but which is not aromatic (also referred to herein as “carbocyclic” or “cycloaliphatic”), that, unless otherwise specified, has a single point of attachment to the rest of the molecule. Unless otherwise specified, aliphatic groups contain 1-12 aliphatic carbon atoms. In some embodiments, aliphatic groups contain 1-6 aliphatic carbon atoms (e.g., Ci-e). In some embodiments, aliphatic groups contain 1-5 aliphatic carbon atoms (e.g., C1-5). In other embodiments, aliphatic groups contain 1-4 aliphatic carbon atoms (e.g., C1-4). In still other embodiments, aliphatic groups contain 1-3 aliphatic carbon atoms (e.g., C1-3), and in yet other embodiments, aliphatic groups contain 1-2 aliphatic carbon atoms (e.g., C1-2). Suitable aliphatic groups include, but are not limited to, linear or branched, substituted or unsubstituted alkyl, alkenyl, alkynyl groups and hybrids thereof. In some embodiments, “aliphatic” refers to a straight-chain (i.e., unbranched) or branched, optionally substituted hydrocarbon chain that is completely saturated or that contains one or more units of unsaturation that has a single point of attachment to the rest of the molecule.

Alkyl: The term “alkyl”, used alone or as part of a larger moiety, refers to a saturated optionally substituted straight or branched hydrocarbon group having (unless otherwise specified) 1-12, 1-10, 1-8, 1-6, 1-4, 1-3, or 1-2 carbon atoms (e.g., C1-12, CHO, Ci-s, C1-6, C1-4, C1-3, or C1-2). Exemplary alkyl groups include methyl, ethyl, propyl, butyl, pentyl, hexyl, and heptyl.

Alkenyl: The term “alkenyl”, used alone or as part of a larger moiety, refers to an optionally substituted straight or branched hydrocarbon chain having at least one double bond and having (unless otherwise specified) 2-12, 2-10, 2-8, 2-6, 2-4, or 2-3 carbon atoms (e.g., C2-12, C2-10, C2-8, C2-6, C2-4, or C2-3). Exemplary alkenyl groups include ethenyl, propenyl, butenyl, pentenyl, hexenyl, and heptenyl.

Alkynyl: The term “alkynyl”, used alone or as part of a larger moiety, refers to an optionally substituted straight or branched chain hydrocarbon group having at least one triple bond and having (unless otherwise specified) 2-12, 2-10, 2-8, 2-6, 2-4, or 2-3 carbon atoms (e.g., C2-12, C2-10, C2-8, C2-6, C2-4, or C2-3). Exemplary alkynyl groups include ethynyl, propynyl, butynyl, pentynyl, hexynyl, and heptynyl.

Aryl: As used herein, the term “aryl” refers to monocyclic, bicyclic, and polycyclic ring systems having a total of six to fourteen ring members (e.g., Ce-u), wherein at least one ring in the system is aromatic and wherein each ring in the system contains three to seven ring members. The term “aryl” may be used interchangeably with the term “aryl ring”. In some embodiments, “aryl” refers to an aromatic ring system which includes, but not limited to, phenyl, naphthyl, anthracyl and the like, which may bear one or more substituents. Unless otherwise specified, “aryl” groups are hydrocarbons.

Bivalent: As used herein, the term “bivalent” refers to a chemical moiety with two points of attachment to the rest of the molecule. For example, “bivalent C1-6 aliphatic,” refers to bivalent aliphatic groups that are as defined herein, containing 1-6 aliphatic carbon atoms.

Carbocyclyl: As used herein, the terms “carbocyclyl,” “carbocycle,” and “carbocyclic ring” refer to saturated or partially unsaturated cyclic aliphatic monocyclic, bicyclic, or polycyclic ring systems, as described herein, having from 3 to 14 members, wherein the aliphatic ring system is optionally substituted as described herein. Carbocyclic groups include, without limitation, cyclopropyl, cyclobutyl, cyclopentyl, cyclopentenyl, cyclohexyl, cyclohexenyl, cycloheptyl, cycloheptenyl, cyclooctyl, cyclooctenyl, norbomyl, adamantyl, and cyclooctadienyl. In some embodiments, “carbocyclyl” (or “cycloaliphatic”) refers to an optionally substituted monocyclic C3-C8 hydrocarbon, or an optionally substituted C5-C10 bicyclic hydrocarbon that is completely saturated or that contains one or more units of unsaturation, but which is not aromatic, that has a single point of attachment to the rest of the molecule. The term “cycloalkyl” refers to an optionally substituted saturated ring system of about 3 to about 10 ring carbon atoms. In some embodiments, cycloalkyl groups have 3-6 carbons. Exemplary monocyclic cycloalkyl rings include cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, and cycloheptyl. The term “cycloalkenyl” refers to an optionally substituted non-aromatic monocyclic or multicyclic ring system containing at least one carbon-carbon double bond and having about 3 to about 10 carbon atoms. Exemplary monocyclic cycloalkenyl rings include cyclopentenyl, cyclohexenyl, and cycloheptenyl.

Carrier: As used herein, the term “carrier” refers to a diluent, adjuvant, excipient, or vehicle with which a composition is administered. In some embodiments, carriers can include sterile liquids, such as, for example, water and oils, including oils of petroleum, animal, vegetable or synthetic origin, such as, for example, peanut oil, soybean oil, mineral oil, sesame oil and the like. In some embodiments, carriers are or include one or more solid components.

Excipient: As used herein, the term “excipient” refers to a non-therapeutic agent that may be included in a pharmaceutical composition, for example, to provide or contribute to a desired consistency or stabilizing effect. Suitable pharmaceutical excipients include, for example, starch, glucose, lactose, sucrose, gelatin, malt, rice, flour, chalk, silica gel, sodium stearate, glycerol monostearate, talc, sodium chloride, dried skim milk, glycerol, propylene, glycol, water, ethanol and the like.

Heteroaryl: As used herein, the terms “heteroaryl” and “heteroar-”, used alone or as part of a larger moiety, e.g., “heteroaralkyl”, or “heteroaralkoxy”, refer to monocyclic or bicyclic ring groups having 5 to 10 ring atoms (e.g., 5- to 6-membered monocyclic heteroaryl or 9- to 10-membered bicyclic heteroaryl); having 6, 10, or 14 n electrons shared in a cyclic array; and having, in addition to carbon atoms, from one to five heteroatoms. Exemplary heteroaryl groups include, without limitation, thienyl, furanyl, pyrrolyl, imidazolyl, pyrazolyl, triazolyl, tetrazolyl, oxazolyl, isoxazolyl, oxadiazolyl, thiazolyl, isothiazolyl, thiadiazolyl, pyridyl, pyridonyl, pyridazinyl, pyrimidinyl, pyrazinyl, indolizinyl, purinyl, naphthyridinyl, pteridinyl, imidazo[l,2-a]pyrimidinyl, imidazo[l,2-a]pyridinyl, thienopyrimidinyl, triazolopyridinyl, and benzoisoxazolyl. The terms “heteroaryl” and “heteroar-”, as used herein, also include groups in which a heteroaromatic ring is fused to one or more aryl, cycloaliphatic, or heterocyclyl rings, where the radical or point of attachment is on the heteroaromatic ring (i.e., a bicyclic heteroaryl ring having 1 to 3 heteroatoms). Nonlimiting examples include indolyl, isoindolyl, benzothienyl, benzofuranyl, dibenzofuranyl, indazolyl, benzimidazolyl, benzothiazolyl, benzothiadiazolyl, benzoxazolyl, quinolyl, isoquinolyl, cinnolinyl, phthalazinyl, quinazolinyl, quinoxalinyl, 47/ quinolizinyl. carbazolyl, acridinyl, phenazinyl, phenothiazinyl, phenoxazinyl, tetrahydroquinolinyl, tetrahydroisoquinolinyl, pyrido[2,3-b]-l,4-oxazin-3(4H)-one, and benzoisoxazolyl. The term “heteroaryl” may be used interchangeably with the terms “heteroaryl ring”, “heteroaryl group”, or “heteroaromatic”, any of which terms include rings that are optionally substituted.

Heteroatom: As used herein, the term “heteroatom” as used herein refers to nitrogen, oxygen, or sulfur, and includes any oxidized form of nitrogen or sulfur, and any quatemized form of a basic nitrogen.

Heterocycle: As used herein, the terms “heterocycle”, “heterocyclyl”, and “heterocyclic ring” are used interchangeably and refer to a stable 3- to 8-membered monocyclic or 5- to 10-membered bicyclic heterocyclic moiety that is either saturated or partially unsaturated, and having, in addition to carbon atoms, one or more, such as one to four, heteroatoms, as defined above. When used in reference to a ring atom of a heterocycle, the term "nitrogen" includes a substituted nitrogen. As an example, in a saturated or partially unsaturated ring having 0-3 heteroatoms selected from oxygen, sulfur or nitrogen, the nitrogen may be N (as in 3,4-dihydro-2H-pyrrolyl), NH (as in pyrrolidinyl), or NR ⁺ (as in N- substituted pyrrolidinyl). A heterocyclic ring can be attached to its pendant group at any heteroatom or carbon atom that results in a stable structure and any of the ring atoms can be optionally substituted. Examples of such saturated or partially unsaturated heterocyclic radicals include, without limitation, tetrahydrofuranyl, tetrahydrothienyl, piperidinyl, decahydroquinolinyl, oxazolidinyl, piperazinyl, dioxanyl, dioxolanyl, diazepinyl, oxazepinyl, thiazepinyl, morpholinyl, and thiamorpholinyl. A heterocyclyl group may be mono-, bi-, tri-, or polycyclic, preferably mono-, bi-, or tricyclic, more preferably mono- or bicyclic. A bicyclic heterocyclic ring also includes groups in which the heterocyclic ring is fused to one or more aryl, heteroaryl, or cycloaliphatic rings. Exemplary bicyclic heterocyclic groups include indolinyl, isoindolinyl, benzodioxolyl, 1,3-dihydroisobenzofuranyl, 2,3- dihydrobenzofuranyl, and tetrahydroquinolinyl. A bicyclic heterocyclic ring can also be a spirocyclic ring system (e.g., 7- to 11-membered spirocyclic fused heterocyclic ring having, in addition to carbon atoms, one or more heteroatoms as defined above (e.g., one, two, three or four heteroatoms)).

Partially Unsaturated: As used herein, the term “partially unsaturated”, when referring to a ring moiety, means a ring moiety that includes at least one double or triple bond between ring atoms. The term “partially unsaturated” is intended to encompass rings having multiple sites of unsaturation, but is not intended to include aromatic (e.g., aryl or heteroaryl) moieties, as herein defined.

Patient or subject: As used herein, the term “patient” or “subject” refers to any organism to which a provided composition is or may be administered, e.g., for experimental, diagnostic, prophylactic, cosmetic, and/or therapeutic purposes. Typical patients or subjects include animals (e.g., mammals such as mice, rats, rabbits, non-human primates, and/or humans). In some embodiments, a patient is a human. In some embodiments, a patient or a subject is suffering from or susceptible to one or more disorders or conditions. In some embodiments, a patient or subject displays one or more symptoms of a disorder or condition. In some embodiments, a patient or subject has been diagnosed with one or more disorders or conditions. In some embodiments, a patient or a subject is receiving or has received certain therapy to diagnose and/or to treat a disease, disorder, or condition.

Pharmaceutical composition: As used herein, the term “pharmaceutical composition” refers to an active agent, formulated together with one or more pharmaceutically acceptable carriers. In some embodiments, active agent is present in unit dose amount appropriate for administration in a therapeutic regimen that shows a statistically significant probability of achieving a predetermined therapeutic effect when administered to a relevant population. In some embodiments, pharmaceutical compositions may be specially formulated for administration in solid or liquid form, including those adapted for the following: oral administration, for example, drenches (aqueous or non-aqueous solutions or suspensions), tablets, e.g., those targeted for buccal, sublingual, and systemic absorption, boluses, powders, granules, pastes for application to the tongue; parenteral administration, for example, by subcutaneous, intramuscular, intravenous or epidural injection as, for example, a sterile solution or suspension, or sustained-release formulation; topical application, for example, as a cream, ointment, or a controlled-release patch or spray applied to the skin, lungs, or oral cavity; intravaginally or intrarectally, for example, as a pessary, cream, or foam; sublingually; ocularly; transdermally; or nasally, pulmonary, and to other mucosal surfaces.

Pharmaceutically acceptable: As used herein, the phrase “pharmaceutically acceptable” refers to those compounds, materials, compositions, and/or dosage forms which are, within the scope of sound medical judgment, suitable for use in contact with the tissues of human beings and animals without excessive toxicity, irritation, allergic response, or other problem or complication, commensurate with a reasonable benefit/risk ratio.

Pharmaceutically acceptable carrier: As used herein, the term “pharmaceutically acceptable carrier” means a pharmaceutically -acceptable material, composition or vehicle, such as a liquid or solid filler, diluent, excipient, or solvent encapsulating material, involved in carrying or transporting the subject compound from one organ, or portion of the body, to another organ, or portion of the body. Each carrier must be “acceptable” in the sense of being compatible with the other ingredients of the formulation and not injurious to the patient. Some examples of materials which can serve as pharmaceutically -acceptable carriers include: sugars, such as lactose, glucose and sucrose; starches, such as com starch and potato starch; cellulose, and its derivatives, such as sodium carboxymethyl cellulose, ethyl cellulose and cellulose acetate; powdered tragacanth; malt; gelatin; talc; excipients, such as cocoa butter and suppository waxes; oils, such as peanut oil, cottonseed oil, safflower oil, sesame oil, olive oil, com oil and soybean oil; glycols, such as propylene glycol; polyols, such as glycerin, sorbitol, mannitol and polyethylene glycol; esters, such as ethyl oleate and ethyl laurate; agar; buffering agents, such as magnesium hydroxide and aluminum hydroxide; alginic acid; pyrogen-free water; isotonic saline; Ringer’s solution; ethyl alcohol; pH buffered solutions; polyesters, polycarbonates and/or polyanhydrides; and other non-toxic compatible substances employed in pharmaceutical formulations.

Pharmaceutically acceptable salt: As used herein, the term “pharmaceutically acceptable salt” refers to salts of such compounds that are appropriate for use in pharmaceutical contexts, i.e., salts which are, within the scope of sound medical judgment, suitable for use in contact with the tissues of humans and lower animals without undue toxicity, irritation, allergic response and the like, and are commensurate with a reasonable benefit/risk ratio. Pharmaceutically acceptable salts are well known in the art. For example, S. M. Berge, et al. describes pharmaceutically acceptable salts in detail in J. Pharmaceutical Sciences, 66: 1-19 (1977).

Prevent or prevention: As used herein, the term “prevent” or “prevention,” when used in connection with the occurrence of a disease, disorder, and/or condition, refers to reducing the risk of developing the disease, disorder and/or condition and/or to delaying onset of one or more characteristics or symptoms of the disease, disorder or condition. Prevention may be considered complete when onset of a disease, disorder or condition has been delayed for a predefined period of time.

Substituted or optionally substituted: As described herein, compounds of this disclosure may contain “optionally substituted” moieties. In general, the term “substituted,” whether preceded by the term “optionally” or not, means that one or more hydrogens of the designated moiety are replaced with a suitable substituent (i.e., as described below for optionally substituted groups). “Substituted” applies to one or more hydrogens that are either

Unless otherwise indicated, an “optionally substituted” group may have a suitable substituent at each substitutable position of the group, and when more than one position in any given structure may be substituted with more than one substituent selected from a specified group, the substituent may be either the same or different at every position. Combinations of substituents envisioned by this invention are preferably those that result in the formation of stable or chemically feasible compounds. The term “stable,” as used herein, refers to compounds that are not substantially altered when subjected to conditions to allow fortheir production, detection, and, in certain embodiments, their recovery, purification, and use for one or more of the purposes provided herein. Groups described as being “substituted” preferably have between 1 and 4 substituents, more preferably 1 or 2 substituents. Groups described as being “optionally substituted” may be unsubstituted or be “substituted” as described above.

Suitable monovalent substituents on a substitutable carbon atom of an “optionally substituted” group are independently halogen; -(CH ₂ )o-4R°; -(CThj^OR ⁰ : -0(CH ₂ )o-4R°, -O- (CH ₂ ) ₀ ^C(O)OR°; -(CH ₂ )O-4CH(OR°) ₂ ; -(CH ₂ ) _O-4 SR°; -(CH ₂ ) ₀ ^Ph, which may be substituted with R°; -(CH ₂ )O^O(CH ₂ )O-I Ph which may be substituted with R°; -CH=CHPh, which may be substituted with R°; -(CH ₂ )o-40(CH ₂ )o-i-pyridyl which may be substituted with R°; -NO ₂ ; -CN; -N ₃ ; -(CH ₂ ) _O ^N(R°) ₂ ; -(CH ₂ ) ₀ ^N(R ^O )C(O)R°; -N(R°)C(S)R°; -(CH ₂ ) _O _ ₄ N(R°)C(0)NR ^O ₂ ; -N(R ^O )C(S)NR° ₂ ; -(CH ₂ ) _0-4 N(R ^O )C(O)OR°; - N(R°)N(R°)C(O)R°; -N(R°)N(R°)C(O)NR° ₂ ; -N(R°)N(R°)C(O)OR°; -(CH ₂ ) _0-4 C(O)R°; - C(S)R°; -(CH ₂ ) ₀ ^C(O)OR°; -(CH ₂ ) ₀ ^C(O)SR ^O ; -(CH ₂ ) ₀ ^C(O)OSIR° ₃ ; -(CH ₂ ) ₀ ^OC(O)R ^O ; - OC(0)(CH ₂ )O- ₄ SR°; -(CH ₂ ) ₀ ^SC(O)R ^O ; -(CH ₂ ) _0-4 C(O)NR ^O ₂ ; -C(S)NR ^O ₂ ; -C(S)SR°; - SC(S)SR°, -(CH ₂ ) ₀ ^OC(O)NR° ₂ ; -C(O)N(OR°)R°; -C(O)C(O)R°; -C(0)CH ₂ C(0)R ^O ; - C(NOR°)R°; -(CH ₂ )O- ₄ SSR°; -(CH ₂ ) ₀ ^S(O) ₂ R ^O ; -(CH ₂ ) ₀ ^S(O)(=NR ^O )R°; -(CH ₂ ) _O _ ₄ S(O) ₂ OR°; -(CH ₂ )O- ₄ OS(0) ₂ R°; -(CH ₂ ) _0.4 -S(O) ₂ NR° ₂ ; -(CH ₂ ) _0.4 S(O)(=NR ^O )NR° ₂ ; -(CH ₂ ) _O _ ₄ S(O)R°; -N(R°)S(0) ₂ NR ^O ₂ ; -N(R°)S(0) ₂ R ^O ; -N(R°)S(O)(=NR°)R°; -N(OR°)R°; - C(NH)NR° ₂ ; -P(O) ₂ R°; -P(O)R° ₂ ; -OP(O)R° ₂ ; -OP(O)(OR°) ₂ ; -SiR° ₃ ; -(C _M straight or branched alkylene)O-N(R°) ₂ ; or -(Ci^ straight or branched alkylene)C(O)O-N(R°) ₂ , wherein each R° may be substituted as defined below and is independently hydrogen, Ci-6 aliphatic, - CH2PI1, -0(CH2)o-iPh, -CH2-(5- to 6-membered heteroaryl ring), or a 3- to 6-membered saturated, partially unsaturated, or aryl ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or, notwithstanding the definition above, two independent occurrences of R°, taken together with their intervening atom(s), form a 3- to 12-membered saturated, partially unsaturated, or aryl mono- or bicyclic ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur, which may be substituted as defined below.

Suitable monovalent substituents on R° (or the ring formed by taking two independent occurrences of R° together with their intervening atoms), are independently halogen, -(CH ₂ ) _0-2 R*, -(haloR*), -(CH ₂ ) _0-2 OH, -(CH ₂ ) _0-2 OR*, -(CH ₂ ) ₀ _ ₂ CH(OR*) ₂ , -O(haloR’), -(CH ₂ ) _0-2 CN, -N ₃ , -(CH ₂ ) _0-2 C(O)R*, -(CH ₂ ) _0-2 C(O)OH, -(CH ₂ ) _O _ ₂ C(O)OR*, -(CH ₂ ) ₀ _2C(O)NH ₂ , -(CH ₂ ) _0-2 C(O)NHR*, -(CH ₂ ) ₀ -2C(O)NR*2, -(CH ₂ ) _O-2 SR*, - (CH ₂ ) _0-2 SH, -(CH ₂ ) _O _2NH ₂ , -(CH ₂ ) _O-2 NHR*, -(CH ₂ ) _O _2NR*2, -(CH ₂ ) _0-2 NHC(O)R*, -(CH ₂ )O- 2NR*C(O)R*, -NO2, -SiR*3, -OSiR*3, -C(O)SR* -(C1-4 straight or branched alkylcnc)C(O)OR*. or -SSR* wherein each R* is unsubstituted or where preceded by “halo” is substituted only with one or more halogens, and is independently selected from Ci- 4 aliphatic, -CH2PI1, -0(CH2)o-iPh, or a 3 - to 6-membered saturated, partially unsaturated, or aryl ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur.

Suitable divalent substituents on a saturated carbon atom of R° include =0 and =S.

Suitable divalent substituents on a saturated carbon atom of an “optionally substituted” group include the following: =0 (“oxo”), =S, =NNR*2, =NNHC(0)R*, =NNHC(0)0R*, =NNHS(O) ₂ R*, =NR*, =N0R*, -O(C(R* ₂ )) _2-3 O-, or -S(C(R* ₂ ))2- ₃ S-, wherein each independent occurrence of R* is selected from hydrogen, C1-6 aliphatic which may be substituted as defined below, or an unsubstituted 3- to 6-membered saturated, partially unsaturated, or aryl ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur. Suitable divalent substituents that are bound to vicinal substitutable carbons of an “optionally substituted” group include: -O(CR*2)2-3O-, wherein each independent occurrence of R* is selected from hydrogen, C1-6 aliphatic which may be substituted as defined below, or an unsubstituted 5-6-membered saturated, partially unsaturated, or aryl ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur.

Suitable substituents on the aliphatic group of R* include halogen, -

R*, -(haloR*), -OH, -OR*, -O(haloR*), -CN, -C(O)OH, -C(O)OR*, -NH ₂ , -NHR*, -NR* ₂ , or -NO2, wherein each R* is unsubstituted or where preceded by “halo” is substituted only with one or more halogens, and is independently C 1-4 aliphatic, -CH2PI1, -0(CH2)o-iPh, or a 3- to 6-membered saturated, partially unsaturated, or aryl ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur.

Suitable substituents on a substitutable nitrogen of an “optionally substituted” group include -R ^T , -W ₂ , -C(O)R ^T , -C(O)OR ^t , -C(O)C(O)R ^t , - C(O)CH ₂ C(OM -S(O) ₂ R ^T , -S(O) ₂ NRt ₂ , -C(S)W ₂ , -C(NH)NR ^: ₂ . or -N(Rt)S(O) ₂ R ^T ; wherein each R ^: is independently hydrogen, Ci-6 aliphatic which may be substituted as defined below, or an unsubstituted 3- to 6-membered saturated, partially unsaturated, or aryl ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or, notwithstanding the definition above, two independent occurrences of R ^: . taken together with their intervening atom(s) form an unsubstituted 3- to 12-membered saturated, partially unsaturated, or aryl mono- or bicyclic ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur.

Suitable substituents on the aliphatic group of R ^: are independently halogen, - R*, -(haloR*), -OH, -OR*, -O(haloR*), -CN, -C(O)OH, -C(O)OR*, -NH ₂ , -NHR*, -NR* ₂ , or -NO ₂ , wherein each R* is unsubstituted or where preceded by “halo” is substituted only with one or more halogens, and is independently Ci^ aliphatic, -CH ₂ Ph, -0(CH ₂ )o-iPh, or a 3- to 6-membered saturated, partially unsaturated, or aryl ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur.

Treat: As used herein, the term “treat” (also “treatment” or “treating”) refers to any administration of a therapy that partially or completely alleviates, ameliorates, relives, inhibits, delays onset of, reduces severity of, and/or reduces incidence of one or more symptoms, features, and/or causes of a particular disease, disorder, and/or condition. In some embodiments, such treatment may be of a subject who does not exhibit signs of the relevant disease, disorder and/or condition and/or of a subject who exhibits only early signs of the disease, disorder, and/or condition. Alternatively or additionally, such treatment may be of a subject who exhibits one or more established signs of the relevant disease, disorder and/or condition. In some embodiments, treatment may be of a subject who has been diagnosed as suffering from the relevant disease, disorder, and/or condition.

Provided Compounds

In some embodiments, the present disclosure provides a compound of Formula I: or a pharmaceutically acceptable salt thereof, wherein:

- is a single or double bond;

X is N or C;

Y is N or C;

Ring A is phenyl, 5- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1-3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, or 5- to 6- membered monocyclic heteroaryl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur; each L ^A is independently a covalent bond or optionally substituted bivalent Ci-6 aliphatic; each R ^A is independently oxo, halogen, -CN, -OR ^A1 , -SR ^A1 , -N(R ^A1 )2, -C(O)R ^A1 , -C(O)OR ^A1 , -C(O)N(R ^A1 ) ₂ , -C(O)NR ^A1 (OR ^A1 ), -OC(O)R ^A1 -OC(O)N(R ^A1 ) ₂ , -OC(O)OR ^A1 , - OSO ₂ R ^A1 , -OSO ₂ N(R ^A1 ) ₂ , -N(R ^A1 )C(O)R ^A1 , -NR ^A1 C(O)OR ^A1 , -NR ^A1 C(O)N(R ^A1 ) ₂ , - N(R ^A1 )SO ₂ R ^A1 , -NR ^A1 S(O) ₂ N(R ^A1 ) ₂ , -NR ^A1 OR ^A1 , -NR ^A1 S(O)R ^A1 , -NR ^A1 S(O)N(R ^A1 ) ₂ , - S(O)R ^A1 , -SO ₂ R ^A1 , -S(O)N(R ^A1 ) ₂ , -SO ₂ N(R ^A1 ) ₂ , -SO ₃ R ^A1 , -C(=NR ^m )R ^A1 , -C(=NR ^m )N(R ^A1 ) ₂ , - NR ^A1 C(=NR ^m )R ^A1 , -NR ^A1 C(=NR ^m )N(R ^A1 ) ₂ , -NR ^A1 S(O)(=NR ^m )R ^A1 , - NR ^A1 S(O)(=NR ^m )N(R ^A1 ) ₂ , -OS(O)(=NR ^m )R ^A1 , -S(O)(=NR ^m )R ^A1 , -S(O)(=NR ^m )N(R ^A1 ) ₂ , - P(O)(R ^A1 ) ₂ , CI-6 aliphatic, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 6- membered monocyclic heteroaryl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, or 8- to 10-membered bicyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein each of the C1-6 aliphatic, 3- to 7-membered monocyclic carbocyclyl, 5- to 10-membered bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7- membered monocyclic heterocyclyl, 5- to 10-membered bicyclic heterocyclyl, 5- to 6- membered monocyclic heteroaryl, and 8- to 10-membered bicyclic heteroaryl of R ^A is independently optionally substituted with 1, 2, 3, 4, 5, or 6 R ^AG substituents; or two L ^A -R ^A taken together with the atoms to which they are attached form a 3 - to 7- membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1-3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 6-membered monocyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, or 8- to 10- membered bicyclic heteroaryl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein each of the 3- to 7-membered monocyclic carbocyclyl, 5- to 10- membered bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7-membered monocyclic heterocyclyl, 5- to 10-membered bicyclic heterocyclyl, 5- to 6-membered monocyclic heteroaryl, and 8- to 10-membered bicyclic heteroaryl is independently optionally substituted with 1, 2, 3, 4, 5, or 6 R ^AG substituents;

R ¹ is hydrogen or an optionally substituted group selected from C1-6 aliphatic, 3- to 7- membered saturated or partially unsaturated monocyclic carbocyclyl, phenyl, 3- to 7- membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, and 5- to 6-membered monocyclic heteroaryl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur; or R ¹ and one of L ^A -R ^A taken together with the atoms to which they are attached form a 3 - to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1-3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, or 5- to 14-membered saturated or partially unsaturated bicyclic heterocyclyl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein each of the 3- to 7-membered monocyclic heterocyclyl and 5- to 14-membered bicyclic heterocyclyl is independently optionally substituted with 1, 2, 3, 4, 5, or 6 R ^AG substituents;

Z is N or CR ² ;

U is N or CR ⁴ ;

R ² and R ⁴ are each independently hydrogen, halogen, -CN, -OR, -SR, -N(R)2, - C(O)R, -C(O)N(R) ₂ , -C(O)NR(OR), -OC(O)R, -OC(O)N(R) ₂ , -OC(O)OR, -OSO ₂ R, - OSO ₂ N(R) ₂ , -N(R)C(O)R, -NRC(O)OR, -NRC(O)N(R) ₂ , -N(R)SO ₂ R, -NRS(O) ₂ N(R) ₂ , - NROR, -NRS(O)R, -NRS(O)N(R) ₂ , -S(O)R, -SO ₂ R, -S(O)N(R) ₂ , -SO ₂ N(R) ₂ , -SO ₃ R, - C(=NR ^m )R, -C(=NR ^m )N(R) ₂ , -NRC(=NR ^m )R, -NRC(=NR ^m )N(R) ₂ , -NRS(O)(=NR ^m )R, - NRS(O)(=NR ^m )N(R) ₂ , -OS(O)(=NR ^m )R, -S(O)(=NR ^m )R, -S(O)(=NR ^m )N(R) ₂ , -P(O)(R) ₂ , or an optionally substituted group selected from C1-6 aliphatic, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, phenyl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, and 5- to 6-membered monocyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur;

R ³ is -F, -Cl, -CN, -OR, -SR, -N(R) ₂ , -C(O)R, -C(O)N(R) ₂ , -C(O)NR(OR), -OC(O)R, -OC(O)N(R) ₂ , -OC(O)OR, -OSO ₂ R, -OSO ₂ N(R) ₂ , -N(R)C(O)R, -NRC(O)OR, - NRC(O)N(R) ₂ , -N(R)SO ₂ R, -NRS(O) ₂ N(R) ₂ , -NROR, -NRS(O)R, -NRS(O)N(R) ₂ , -S(O)R, - SO ₂ R, -S(O)N(R) ₂ , -SO ₂ N(R) ₂ , -SO ₃ R, -C(=NR ^m )R, -C(=NR ^m )N(R) ₂ , -NRC(=NR ^m )R, - NRC(=NR ^m )N(R) ₂ , -NRS(O)(=NR ^m )R, -NRS(O)(=NR ^m )N(R) ₂ , -OS(O)(=NR ^m )R, - S(O)(=NR ^m )R, -S(O)(=NR ^m )N(R) ₂ , -P(O)(R) ₂ , or an optionally substituted group selected from C1-6 aliphatic, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, phenyl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, and 5- to 6-membered monocyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur;

R ⁵ is -L ⁵ -R ^5A ;

L ⁵ is a covalent bond or optionally substituted bivalent C1-6 aliphatic; or L ⁵ and one of L ^A -R ^A taken together with the atoms to which they are attached form a 3 - to 16-membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 16- membered saturated or partially unsaturated bicyclic carbocyclyl, 3- to 16-membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, or 5- to 16-membered saturated or partially unsaturated bicyclic heterocyclyl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein each of the 3- to 16-membered monocyclic carbocyclyl, 5- to 16-membered bicyclic carbocyclyl, 3- to 16 -membered monocyclic heterocyclyl, and 5- to 16-membered bicyclic heterocyclyl is independently optionally substituted with 1, 2, 3, 4, 5, or 6 R ^AG substituents;

R ^5A is -OR ^5A11 , -SR ^5A1 , -NHR ^5A12 , -N(R ^5A12 ) ₂ , -C(O)R ^5A1 , -C(O)N(R ^5A1 ) ₂ , - C(O)NR ^5A1 (OR ^5A1 ), -OC(O)R ^5A1 , -OC(O)N(R ^5A1 ) ₂ , -OC(O)OR ^5A1 , -OSO ₂ R ^5A1 , - OSO ₂ N(R ^5A1 ) ₂ , -N(R ^5A1 )C(O)R ^5A1 , -NR ^5A1 C(O)OR ^5A1 , -NR ^5A1 C(O)N(R ^5A1 ) ₂ , - N(R ^5A1 )SO ₂ R ^5A1 , -NR ^5A1 S(O) ₂ N(R ^5A1 ) ₂ , -NR ^5A1 OR ^5A1 , -NR ^5A1 S(O)R ^5A1 , -NR ^5A1 S(O)N(R ^5A1 ) ₂ , -S(O)R ^5A1 , -SO ₂ R ^5A1 , -S(O)N(R ^5A1 ) ₂ , -SO ₂ N(R ^5A1 ) ₂ , -SO ₃ R ^5A1 , -C(=NR ^m )R ^5A1 , - C(=NR ^m )N(R ^5A1 ) ₂ , -NR ^5A1 C(=NR ^m )R ^5A1 , -NR ^5A1 C(=NR ^m )N(R ^5A1 ) ₂ , -NR ^5A1 S(O)(=NR ^m )R ^5A1 , - NR ^5A1 S(O)(=NR ^m )N(R ^5A1 ) ₂ , -OS(O)(=NR ^m )R ^5A1 , -S(O)(=NR ^m )R ^5A1 , -S(O)(=NR ^m )N(R ^5A1 ) ₂ , - P(O)(R ^5A1 ) ₂ , methyl, C ₂ -6 aliphatic, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 6-membered monocyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, or 8- to 10-membered bicyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein each of the C ₂ -6 aliphatic, 3- to 7-membered monocyclic carbocyclyl, 5- to 10-membered bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7- membered monocyclic heterocyclyl, 5- to 10-membered bicyclic heterocyclyl, 5- to 6- membered monocyclic heteroaryl, and 8- to 10-membered bicyclic heteroaryl of R ^5A is independently optionally substituted with 1, 2, 3, 4, 5, or 6 R ^5AG substituents; or the methyl of R ^5A is substituted with 1, 2, or 3 R ^5AG substituents;

R ^A1 and R ^5A1 are each independently hydrogen, C1-6 aliphatic, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7- membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 6-membered monocyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, or 8- to 10-membered bicyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein each of the C1-6 aliphatic, 3- to 7-membered monocyclic carbocyclyl, 5- to 10-membered bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7- membered monocyclic heterocyclyl, 5- to 10-membered bicyclic heterocyclyl, 5- to 6- membered monocyclic heteroaryl, and 8- to 10-membered bicyclic heteroaryl of R ^A1 or R ^5A1 is independently optionally substituted with 1, 2, 3, 4, 5, or 6 R ^G1 substituents; or two R ^A1 when attached to the same nitrogen atom are taken together to form an optionally substituted ring selected from 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 0-2 additional heteroatoms independently selected from nitrogen, oxygen, and sulfur, and 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 0-3 additional heteroatoms independently selected from nitrogen, oxygen, and sulfur; or two R ^5A1 when attached to the same nitrogen atom are taken together to form an optionally substituted ring selected from 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 0-2 additional heteroatoms independently selected from nitrogen, oxygen, and sulfur, and 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 0-3 additional heteroatoms independently selected from nitrogen, oxygen, and sulfur;

R ^5A11 is methyl, C2-6 aliphatic, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 6-membered monocyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, or 8- to 10-membered bicyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein each of the C2-6 aliphatic, 3- to 7-membered monocyclic carbocyclyl, 5- to 10-membered bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7- membered monocyclic heterocyclyl, 5- to 10-membered bicyclic heterocyclyl, 5- to 6- membered monocyclic heteroaryl, and 8- to 10-membered bicyclic heteroaryl of R ^5A11 is independently optionally substituted with 1, 2, 3, 4, 5, or 6 R ^G1 substituents; or the methyl of R ^5A11 is substituted with 1, 2, or 3 R ^G1 substituents; each R ^5A12 is independently C1-6 aliphatic, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1-3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 6-membered monocyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, or 8- to 10-membered bicyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein each of the C1-6 aliphatic, 3- to 7-membered monocyclic carbocyclyl, 5- to 10-membered bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7- membered monocyclic heterocyclyl, 5- to 10-membered bicyclic heterocyclyl, 5- to 6- membered monocyclic heteroaryl, and 8- to 10-membered bicyclic heteroaryl of R ^5A12 is independently optionally substituted with 1, 2, 3, 4, 5, or 6 R ^G1 substituents; or two R ^5A12 when attached to the same nitrogen atom are taken together to form an optionally substituted ring selected from 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 0-2 additional heteroatoms independently selected from nitrogen, oxygen, and sulfur, and 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 0-3 additional heteroatoms independently selected from nitrogen, oxygen, and sulfur;

R ^AG , R ^5AG , and R ^G1 are each independently halogen, -CN, -OR, -SR, -N(R)2, -C(O)R, -C(O)OR, -C(O)N(R) ₂ , -C(O)NR(OR), -OC(O)R, -OC(O)N(R) ₂ , -OC(O)OR, -OSO ₂ R, - OSO ₂ N(R) ₂ , -N(R)C(O)R, -NRC(O)OR, -NRC(O)N(R) ₂ , -N(R)SO ₂ R, -NRS(O) ₂ N(R) ₂ , - NROR, -NRS(O)R, -NRS(O)N(R) ₂ , -S(O)R, -SO ₂ R, -S(O)N(R) ₂ , -SO ₂ N(R) ₂ , -SO ₃ R, - C(=NR ^m )R, -C(=NR ^m )N(R) ₂ , -NRC(=NR ^m )R, -NRC(=NR ^m )N(R) ₂ , -NRS(O)(=NR ^m )R, - NRS(O)(=NR ^m )N(R) ₂ , -OS(O)(=NR ^m )R, -S(O)(=NR ^m )R, -S(O)(=NR ^m )N(R) ₂ , -P(O)(R) ₂ , or an optionally substituted group selected from C1-6 aliphatic, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1-3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 6-membered monocyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, and 8- to 10- membered bicyclic heteroaryl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur; each R is independently hydrogen or an optionally substituted group selected from Ci- 6 aliphatic, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic carbocyclyl, phenyl, 8- to 10- membered bicyclic aryl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 6-membered monocyclic heteroaryl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, and 8- to 10-membered bicyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur; or two R when attached to the same nitrogen atom are taken together to form an optionally substituted ring selected from 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 0-2 additional heteroatoms independently selected from nitrogen, oxygen, and sulfur, and 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 0-3 additional heteroatoms independently selected from nitrogen, oxygen, and sulfur; each R ^m is independently -OH, -CN, or R; and n is 0, 1, 2, 3, or 4.

In some embodiments of the previous embodiment, L ⁵ and one of L ^A -R ^A taken together with the atoms to which they are attached form a 3 - to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic carbocyclyl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, or 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein each of the 3- to 7-membered monocyclic carbocyclyl, 5- to 10-membered bicyclic carbocyclyl, 3- to 7-membered monocyclic heterocyclyl, and 5- to 10-membered bicyclic heterocyclyl is independently optionally substituted with 1, 2, 3, 4, 5, or 6 R ^AG substituents.

In some embodiments: is a single or double bond;

X is N or C;

Y is N or C;

Ring A is phenyl, 5- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1-3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, or 5- to 6- membered monocyclic heteroaryl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur; each L ^A is independently a covalent bond or a bivalent C1-6 aliphatic, wherein the bivalent C1-6 aliphatic is optionally substituted with 1, 2, 3, or 4 independently selected R ^LA1 substituents; each R ^A is independently oxo, halogen, -CN, -OR ^A1 , -SR ^A1 , -N(R ^A1 )2, -C(O)R ^A1 , -C(O)OR ^A1 , -C(O)N(R ^A1 ) ₂ , -C(O)NR ^A1 (OR ^A1 ), -OC(O)R ^A1 -OC(O)N(R ^A1 ) ₂ , -OC(O)OR ^A1 , - OSO ₂ R ^A1 , -OSO ₂ N(R ^A1 ) ₂ , -N(R ^A1 )C(O)R ^A1 , -NR ^A1 C(O)OR ^A1 , -NR ^A1 C(O)N(R ^A1 ) ₂ , - N(R ^A1 )SO ₂ R ^A1 , -NR ^A1 S(O) ₂ N(R ^A1 ) ₂ , -NR ^A1 OR ^A1 , -NR ^A1 S(O)R ^A1 , -NR ^A1 S(O)N(R ^A1 ) ₂ , - S(O)R ^A1 , -SO ₂ R ^A1 , -S(O)N(R ^A1 ) ₂ , -SO ₂ N(R ^A1 ) ₂ , -SO ₃ R ^A1 , -C(=NR ^m )R ^A1 , -C(=NR ^m )N(R ^A1 ) ₂ , - NR ^A1 C(=NR ^m )R ^A1 , -NR ^A1 C(=NR ^m )N(R ^A1 ) ₂ , -NR ^A1 S(O)(=NR ^m )R ^A1 , - NR ^A1 S(O)(=NR ^m )N(R ^A1 ) ₂ , -OS(O)(=NR ^m )R ^A1 , -S(O)(=NR ^m )R ^A1 , -S(O)(=NR ^m )N(R ^A1 ) ₂ , - P(O)(R ^A1 ) ₂ , CI-6 aliphatic, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 6- membered monocyclic heteroaryl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, or 8- to 10-membered bicyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein each of the C1-6 aliphatic, 3- to 7-membered monocyclic carbocyclyl, 5- to 10-membered bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7- membered monocyclic heterocyclyl, 5- to 10-membered bicyclic heterocyclyl, 5- to 6- membered monocyclic heteroaryl, and 8- to 10-membered bicyclic heteroaryl of R ^A is independently optionally substituted with 1, 2, 3, 4, 5, or 6 R ^AG substituents; or two L ^A -R ^A taken together with the atoms to which they are attached form a 3 - to 7- membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1-3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 6-membered monocyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, or 8- to 10- membered bicyclic heteroaryl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein each of the 3- to 7-membered monocyclic carbocyclyl, 5- to 10- membered bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7-membered monocyclic heterocyclyl, 5- to 10-membered bicyclic heterocyclyl, 5- to 6-membered monocyclic heteroaryl, and 8- to 10-membered bicyclic heteroaryl is independently optionally substituted with 1, 2, 3, 4, 5, or 6 R ^AG substituents;

R ¹ is hydrogen or a group selected from C1-6 aliphatic, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, phenyl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, and 5- to 6-membered monocyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein the Ci-6 aliphatic, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, phenyl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl, and 5- to 6-membered monocyclic heteroaryl are each optionally substituted with 1, 2, 3, or 4 independently selected R ^1A substituents; or R ¹ and one of L ^A -R ^A taken together with the atoms to which they are attached form a 3 - to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1-3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, or 5- to 14-membered saturated or partially unsaturated bicyclic heterocyclyl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein each of the 3- to 7-membered monocyclic heterocyclyl and 5- to 14-membered bicyclic heterocyclyl is independently optionally substituted with 1, 2, 3, 4, 5, or 6 R ^AG substituents;

Z is N or CR ² ;

U is N or CR ⁴ ;

R ² and R ⁴ are each independently hydrogen, halogen, -CN, -OR, -SR, -N(R)2, - C(O)R, -C(O)N(R) ₂ , -C(O)NR(OR), -OC(O)R, -OC(O)N(R) ₂ , -OC(O)OR, -OSO ₂ R, - OSO ₂ N(R) ₂ , -N(R)C(O)R, -NRC(O)OR, -NRC(O)N(R) ₂ , -N(R)SO ₂ R, -NRS(O) ₂ N(R) ₂ , - NROR, -NRS(O)R, -NRS(O)N(R) ₂ , -S(O)R, -SO ₂ R, -S(O)N(R) ₂ , -SO ₂ N(R) ₂ , -SO ₃ R, - C(=NR ^m )R, -C(=NR ^m )N(R) ₂ , -NRC(=NR ^m )R, -NRC(=NR ^m )N(R) ₂ , -NRS(O)(=NR ^m )R, - NRS(O)(=NR ^m )N(R) ₂ , -OS(O)(=NR ^m )R, -S(O)(=NR ^m )R, -S(O)(=NR ^m )N(R) ₂ , -P(O)(R) ₂ , Ci- ₆ aliphatic, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, phenyl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1-3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, and 5- to 6- membered monocyclic heteroaryl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein the C1-6 aliphatic, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, phenyl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl, and 5- to 6-membered monocyclic heteroaryl are each optionally substituted with 1, 2, 3, or 4 independently selected R ^2A substituents;

R ³ is -F, -Cl, -CN, -OR, -SR, -N(R) ₂ , -C(O)R, -C(O)N(R) ₂ , -C(O)NR(OR), -OC(O)R, -OC(O)N(R) ₂ , -OC(O)OR, -OSO ₂ R, -OSO ₂ N(R) ₂ , -N(R)C(O)R, -NRC(O)OR, - NRC(O)N(R) ₂ , -N(R)SO ₂ R, -NRS(O) ₂ N(R) ₂ , -NROR, -NRS(O)R, -NRS(O)N(R) ₂ , -S(O)R, - SO ₂ R, -S(O)N(R) ₂ , -SO ₂ N(R) ₂ , -SO ₃ R, -C(=NR ^m )R, -C(=NR ^m )N(R) ₂ , -NRC(=NR ^m )R, - NRC(=NR ^m )N(R) ₂ , -NRS(O)(=NR ^m )R, -NRS(O)(=NR ^m )N(R) ₂ , -OS(O)(=NR ^m )R, - S(O)(=NR ^m )R, -S(O)(=NR ^m )N(R) ₂ , -P(O)(R) ₂ , C1-6 aliphatic, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, phenyl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, and 5- to 6-membered monocyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein the Ci-6 aliphatic, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, phenyl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl, and 5- to 6-membered monocyclic heteroaryl are each optionally substituted with 1, 2, 3, or 4 independently selected R ^3A substituents;

R ⁵ is -L ⁵ -R ^5A ;

L ⁵ is a covalent bond or a bivalent C1-6 aliphatic, wherein the bivalent C1-6 aliphatic is optionally substituted with 1, 2, 3, or 4 independently selected R ^L5A substituents; or L ⁵ and one of L ^A -R ^A taken together with the atoms to which they are attached form a 3 - to 16-membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 16- membered saturated or partially unsaturated bicyclic carbocyclyl, 3- to 16-membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, or 5- to 16-membered saturated or partially unsaturated bicyclic heterocyclyl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein each of the 3- to 16-membered monocyclic carbocyclyl, 5- to 16-membered bicyclic carbocyclyl, 3- to 16 -membered monocyclic heterocyclyl, and 5- to 16-membered bicyclic heterocyclyl is independently optionally substituted with 1, 2, 3, 4, 5, or 6 R ^AG substituents;

R ^5A is -OR ^5A11 , -SR ^5A1 , -NHR ^5A12 , -N(R ^5A12 ) ₂ , -C(O)R ^5A1 , -C(O)N(R ^5A1 ) ₂ , - C(O)NR ^5A1 (OR ^5A1 ), -OC(O)R ^5A1 , -OC(O)N(R ^5A1 ) ₂ , -OC(O)OR ^5A1 , -OSO ₂ R ^5A1 , - OSO ₂ N(R ^5A1 ) ₂ , -N(R ^5A1 )C(O)R ^5A1 , -NR ^5A1 C(O)OR ^5A1 , -NR ^5A1 C(O)N(R ^5A1 ) ₂ , - N(R ^5A1 )SO ₂ R ^5A1 , -NR ^5A1 S(O) ₂ N(R ^5A1 ) ₂ , -NR ^5A1 OR ^5A1 , -NR ^5A1 S(O)R ^5A1 , -NR ^5A1 S(O)N(R ^5A1 ) ₂ , -S(O)R ^5A1 , -SO ₂ R ^5A1 , -S(O)N(R ^5A1 ) ₂ , -SO ₂ N(R ^5A1 ) ₂ , -SO ₃ R ^5A1 , -C(=NR ^m )R ^5A1 , - C(=NR ^m )N(R ^5A1 ) ₂ , -NR ^5A1 C(=NR ^m )R ^5A1 , -NR ^5A1 C(=NR ^m )N(R ^5A1 ) ₂ , -NR ^5A1 S(O)(=NR ^m )R ^5A1 , - NR ^5A1 S(O)(=NR ^m )N(R ^5A1 ) ₂ , -OS(O)(=NR ^m )R ^5A1 , -S(O)(=NR ^m )R ^5A1 , -S(O)(=NR ^m )N(R ^5A1 ) ₂ , - P(O)(R ^5A1 ) ₂ , methyl, C ₂ -6 aliphatic, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 6-membered monocyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, or 8- to 10-membered bicyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein each of the C2-6 aliphatic, 3- to 7-membered monocyclic carbocyclyl, 5- to 10-membered bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7- membered monocyclic heterocyclyl, 5- to 10-membered bicyclic heterocyclyl, 5- to 6- membered monocyclic heteroaryl, and 8- to 10-membered bicyclic heteroaryl of R ^5A is independently optionally substituted with 1, 2, 3, 4, 5, or 6 R ^5AG substituents; or the methyl of R ^5A is substituted with 1, 2, or 3 R ^5AG substituents;

R ^A1 and R ^5A1 are each independently hydrogen, C1-6 aliphatic, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7- membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 6-membered monocyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, or 8- to 10-membered bicyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein each of the C1-6 aliphatic, 3- to 7-membered monocyclic carbocyclyl, 5- to 10-membered bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7- membered monocyclic heterocyclyl, 5- to 10-membered bicyclic heterocyclyl, 5- to 6- membered monocyclic heteroaryl, and 8- to 10-membered bicyclic heteroaryl of R ^A1 or R ^5A1 is independently optionally substituted with 1, 2, 3, 4, 5, or 6 R ^G1 substituents; or two R ^A1 when attached to the same nitrogen atom are taken together to form a ring selected from 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 0-2 additional heteroatoms independently selected from nitrogen, oxygen, and sulfur, and 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 0-3 additional heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein the 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl and 5- to 10- membered saturated or partially unsaturated bicyclic heterocyclyl are each optionally substituted with 1, 2, 3, or 4 independently selected R ^A11 substituents; or two R ^5A1 when attached to the same nitrogen atom are taken together to form a ring selected from 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 0-2 additional heteroatoms independently selected from nitrogen, oxygen, and sulfur, and 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 0-3 additional heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein the 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl and 5- to 10- membered saturated or partially unsaturated bicyclic heterocyclyl are each optionally substituted with 1, 2, 3, or 4 independently selected R ^A11 substituents;

R ^5A11 is methyl, C2-6 aliphatic, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 6-membered monocyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, or 8- to 10-membered bicyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein each of the C2-6 aliphatic, 3- to 7-membered monocyclic carbocyclyl, 5- to 10-membered bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7- membered monocyclic heterocyclyl, 5- to 10-membered bicyclic heterocyclyl, 5- to 6- membered monocyclic heteroaryl, and 8- to 10-membered bicyclic heteroaryl of R ^5A11 is independently optionally substituted with 1, 2, 3, 4, 5, or 6 R ^G1 substituents; or the methyl of R ^5A11 is substituted with 1, 2, or 3 R ^G1 substituents; each R ^5A12 is independently C1-6 aliphatic, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1-3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 6-membered monocyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, or 8- to 10-membered bicyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein each of the C1-6 aliphatic, 3- to 7-membered monocyclic carbocyclyl, 5- to 10-membered bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7- membered monocyclic heterocyclyl, 5- to 10-membered bicyclic heterocyclyl, 5- to 6- membered monocyclic heteroaryl, and 8- to 10-membered bicyclic heteroaryl of R ^5A12 is independently optionally substituted with 1, 2, 3, 4, 5, or 6 R ^G1 substituents; or two R ^5A12 when attached to the same nitrogen atom are taken together to form a ring selected from 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 0-2 additional heteroatoms independently selected from nitrogen, oxygen, and sulfur, and 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 0-3 additional heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein the 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl and

5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl are each optionally substituted with 1, 2, 3, or 4 independently selected R ^5B12 substituents;

R ^AG , R ^5AG , and R ^G1 are each independently oxo, halogen, -CN, -OR, -SR, -N(R)2, - C(O)R, -C(O)OR, -C(O)N(R) ₂ , -C(O)NR(OR), -OC(O)R, -OC(O)N(R) ₂ , -OC(O)OR, - OSO ₂ R, -OSO ₂ N(R) ₂ , -N(R)C(O)R, -NRC(O)OR, -NRC(O)N(R) ₂ , -N(R)SO ₂ R, - NRS(O) ₂ N(R) ₂ , -NROR, -NRS(O)R, -NRS(O)N(R) ₂ , -S(O)R, -SO ₂ R, -S(O)N(R) ₂ , -SO ₂ N(R) ₂ , -SO ₃ R, -C(=NR ^m )R, -C(=NR ^m )N(R) ₂ , -NRC(=NR ^m )R, -NRC(=NR ^m )N(R) ₂ , - NRS(O)(=NR ^m )R, -NRS(O)(=NR ^m )N(R) ₂ , -OS(O)(=NR ^m )R, -S(O)(=NR ^m )R, - S(O)(=NR ^m )N(R) ₂ , -P(O)(R) ₂ , CI-6 aliphatic, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1-3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 6-membered monocyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, and 8- to 10-membered bicyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein the Ci-6 aliphatic, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic carbocyclyl, phenyl, 8- to 10- membered bicyclic aryl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl, 5- to

6-membered monocyclic heteroaryl, and 8- to 10-membered bicyclic heteroaryl are each optionally substituted with 1, 2, 3, or 4 independently selected R ^AG1 substituents; each R is independently selected from oxo, hydrogen, C1-6 aliphatic, 3- to 7- membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1-3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5 - to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 6-membered monocyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, and 8- to 10- membered bicyclic heteroaryl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein the C1-6 aliphatic, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl, 5- to 6-membered monocyclic heteroaryl, and 8- to 10- membered bicyclic heteroaryl are each optionally substituted with 1, 2, 3, or 4 independently selected R ^N substituents; or two R when attached to the same nitrogen atom are taken together to form a ring selected from 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 0-2 additional heteroatoms independently selected from nitrogen, oxygen, and sulfur, and 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 0-3 additional heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein the 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl and 5- to 10- membered saturated or partially unsaturated bicyclic heterocyclyl are each optionally substituted with 1, 2, 3, or 4 independently selected R ^N substituents; each R ^LA1 , R ^1A , R ^2A , R ^3A , R ^L5A , R ^A11 , R ^5B12 , R ^AG1 , and R ^N is independently selected from oxo, halogen, -(CH ₂ ) ₀ ^R°, -(CH ₂ ) ₀ -4OR°, -O(CH ₂ ) _0.4 R°, -O-(CH ₂ ) _0-4 C(O)OR°, - (CH ₂ ) ₀ ^CH(OR°) ₂ , -(CH ₂ ) _O ^SR°, -(CH ₂ ) ₀ ^Ph, -(CH ₂ )(I- ₄ O(CH ₂ )(I-I Ph. -CH=CHPh, -(CH ₂ ) ₀ _ ₄ 0(CH ₂ )o-i-pyridyl, -NO ₂ , -CN, -N ₃ , -(CH ₂ ) _0-4 N(R°) ₂ , -(CH ₂ ) _0-4 N(R°)C(O)R°, - N(R°)C(S)R°, -(CH ₂ )O^N(R°)C(0)NR° ₂ , -N(R ^O )C(S)NR° ₂ , -(CH ₂ ) ₀ ^N(R ^O )C(O)OR°, - N(R°)N(R°)C(0)R°, -N(R°)N(R°)C(0)NR° ₂ , -N(R°)N(R°)C(0)0R°, -(CH ₂ ) _0-4 C(O)R ^O , - C(S)R°, -(CH ₂ ) _0-4 C(O)OR°, -(CH ₂ )O^C(0)SR°, -(CH ₂ ) ₀ ^C(O)OSIR°3, -(CH ₂ ) _0-4 OC(O)R ^O , - OC(0)(CH ₂ )O_ ₄ SR°, -(CH ₂ )O^SC(0)R°, -(CH ₂ ) _0-4 C(O)NR ^O ₂ , -C(S)NR ^O ₂ , -C(S)SR°, - SC(S)SR°, -(CH ₂ ) ₀ ^OC(O)NR° ₂ , -C(0)N(0R°)R°, -C(O)C(O)R°, -C(0)CH ₂ C(0)R ^O , - C(N0R°)R°, -(CH ₂ )O^SSR°, -(CH ₂ )O^S(0) ₂ R°, -(CH ₂ ) _0-4 S(O)(=NR ^O )R°, -(CH ₂ ) _O _ ₄ S(0) ₂ 0R ^O , -(CH ₂ ) _0-4 OS(O) ₂ R ^O , -(CH ₂ )O. ₄ -S(0) ₂ NR° ₂ , -(CH ₂ ) _0.4 S(O)(=NR ^O )NR° ₂ , -(CH ₂ ) _O _ ₄ S(0)R ^O , -N(R°)S(0) ₂ NR ^O ₂ , -N(R°)S(0) ₂ R ^O , -N(R°)S(0)(=NR°)R°, -N(0R°)R°, - C(NH)NR° ₂ , -P(O) ₂ R°, -P(O)R° ₂ , -OP(O)R° ₂ , -OP(O)(OR°) ₂ , -SiR°3, -(Ci- ₄ straight or branched alkylene)O-N(R°) ₂ , and — (Ci- ₄ straight or branched alkylene)C(O)O-N(R°) ₂ ; each R° is independently oxo, hydrogen, Ci-6 aliphatic, -CH ₂ Ph, -0(CH ₂ )o-iPh, - CH ₂ -(5- to 6-membered heteroaryl ring), or a 3- to 6-membered saturated, partially unsaturated, or aryl ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur; or two independent occurrences of R°, taken together with their intervening atoms, form a 3 - to 12-membered saturated, partially unsaturated, or aryl mono- or bicyclic ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur; each R ^m is independently oxo, -OH, -CN, or R; and n is 0, 1, 2, 3, or 4.

In some embodiments of the previous embodiment:

R ^AG , R ^5AG , and R ^G1 are each independently halogen, -CN, -OR, -SR, -N(R)2, -C(O)R, -C(O)OR, -C(O)N(R) ₂ , -C(O)NR(OR), -OC(O)R, -OC(O)N(R) ₂ , -OC(O)OR, -OSO ₂ R, - OSO ₂ N(R) ₂ , -N(R)C(O)R, -NRC(O)OR, -NRC(O)N(R) ₂ , -N(R)SO ₂ R, -NRS(O) ₂ N(R) ₂ , - NROR, -NRS(O)R, -NRS(O)N(R) ₂ , -S(O)R, -SO ₂ R, -S(O)N(R) ₂ , -SO ₂ N(R) ₂ , -SO ₃ R, - C(=NR ^m )R, -C(=NR ^m )N(R) ₂ , -NRC(=NR ^m )R, -NRC(=NR ^m )N(R) ₂ , -NRS(O)(=NR ^m )R, - NRS(O)(=NR ^m )N(R) ₂ , -OS(O)(=NR ^m )R, -S(O)(=NR ^m )R, -S(O)(=NR ^m )N(R) ₂ , -P(O)(R) ₂ , Ci- ₆ aliphatic, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic carbocyclyl, phenyl, 8- to 10- membered bicyclic aryl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 6-membered monocyclic heteroaryl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, and 8- to 10-membered bicyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein the C1-6 aliphatic, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7- membered saturated or partially unsaturated monocyclic heterocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl, 5- to 6-membered monocyclic heteroaryl, and 8- to 10-membered bicyclic heteroaryl are each optionally substituted with 1, 2, 3, or 4 independently selected R ^AG1 substituents; each R is independently selected from hydrogen, C1-6 aliphatic, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7- membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 6-membered monocyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, and 8- to 10- membered bicyclic heteroaryl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein the C1-6 aliphatic, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl, 5- to 6-membered monocyclic heteroaryl, and 8- to 10- membered bicyclic heteroaryl are each optionally substituted with 1, 2, 3, or 4 independently selected R ^N substituents; or two R when attached to the same nitrogen atom are taken together to form a ring selected from 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 0-2 additional heteroatoms independently selected from nitrogen, oxygen, and sulfur, and 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 0-3 additional heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein the 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl and 5- to 10- membered saturated or partially unsaturated bicyclic heterocyclyl are each optionally substituted with 1, 2, 3, or 4 independently selected R ^N substituents; each R ^LA1 , R ^1A , R ^2A , R ^3A , R ^L5A , R ^A11 , R ^5B12 , R ^AG1 , and R ^N is independently selected from halogen, -(CH ₂ ) ₀ ^R°, -(CH ₂ ) ₀ ^OR°, -O(CH ₂ ) _0.4 R°, -O-(CH ₂ ) _0-4 C(O)OR°, -(CH ₂ ) ₀ _ ₄ CH(OR°) ₂ , -(CH ₂ ) _O-4 SR°, -(CH ₂ ) ₀ ^Ph, -(CH ₂ )(I- ₄ O(CH ₂ )(I-I Ph. -CH=CHPh, -(CH ₂ ) ₀ _ ₄ 0(CH ₂ )o-i-pyridyl, -NO ₂ , -CN, -N ₃ , -(CH ₂ ) _0-4 N(R°) ₂ , -(CH ₂ ) _0-4 N(R°)C(O)R°, - N(R°)C(S)R°, -(CH ₂ )O^N(R°)C(0)NR° ₂ , -N(R ^O )C(S)NR° ₂ , -(CH ₂ ) ₀ ^N(R ^O )C(O)OR°, - N(R°)N(R°)C(O)R°, -N(R°)N(R°)C(O)NR° ₂ , -N(R°)N(R°)C(O)OR°, -(CH ₂ ) _0-4 C(O)R ^O , - C(S)R°, -(CH ₂ ) _0-4 C(O)OR°, -(CH ₂ )O^C(0)SR°, -(CH ₂ ) ₀ ^C(O)OSIR°3, -(CH ₂ ) _0-4 OC(O)R ^O , - OC(0)(CH ₂ )O_ ₄ SR°, -(CH ₂ )O^SC(0)R°, -(CH ₂ ) _0-4 C(O)NR ^O ₂ , -C(S)NR ^O ₂ , -C(S)SR°, - SC(S)SR°, -(CH ₂ ) ₀ ^OC(O)NR° ₂ , -C(O)N(OR°)R°, -C(O)C(O)R°, -C(O)CH ₂ C(O)R°, - C(NOR°)R°, -(CH ₂ )O^SSR°, -(CH ₂ )O^S(0) ₂ R°, -(CH ₂ ) _0-4 S(O)(=NR ^O )R°, -(CH ₂ ) _O _ ₄ S(0) ₂ 0R ^O , -(CH ₂ ) _0-4 OS(O) ₂ R ^O , -(CH ₂ )O. ₄ -S(0) ₂ NR° ₂ , -(CH ₂ ) _0.4 S(O)(=NR ^O )NR° ₂ , -(CH ₂ ) _O _ ₄ S(O)R°, -N(R°)S(0) ₂ NR ^O ₂ , -N(R°)S(O) ₂ R°, -N(R°)S(O)(=NR°)R°, -N(OR°)R°, - C(NH)NR° ₂ , -P(O) ₂ R°, -P(O)R° ₂ , -OP(O)R° ₂ , -OP(O)(OR°) ₂ , -SiR°3, -(Ci- ₄ straight or branched alkylene)O-N(R°) ₂ , and — (Ci- ₄ straight or branched alkylene)C(O)O-N(R°) ₂ ; each R° is independently hydrogen, Ci-6 aliphatic, -CH ₂ Ph, -0(CH ₂ )o-iPh, -CH ₂ -(5- to 6-membered heteroaryl ring), or a 3 - to 6-membered saturated, partially unsaturated, or aryl ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur; or two independent occurrences of R°, taken together with their intervening atoms, form a 3 - to 12-membered saturated, partially unsaturated, or aryl mono- or bicyclic ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur; and each R ^m is independently-OH, -CN, or R.

In some embodiments of the previous embodiments, L ⁵ and one of L ^A -R ^A taken together with the atoms to which they are attached form a 3 - to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic carbocyclyl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, or 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein each of the 3- to 7-membered monocyclic carbocyclyl, 5- to 10-membered bicyclic carbocyclyl, 3- to 7-membered monocyclic heterocyclyl, and 5- to 10-membered bicyclic heterocyclyl is independently optionally substituted with 1, 2, 3, 4, 5, or 6 R ^AG substituents.

In some embodiments:

- is a single or double bond;

X is N or C;

Y is N or C;

Ring A is phenyl or 5- to 6-membered monocyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur; each L ^A is independently a covalent bond or optionally substituted bivalent Ci-6 aliphatic; each R ^A is independently oxo, halogen, -CN, -OR ^A1 , -SR ^A1 , -N(R ^A1 )2, -C(O)R ^A1 , -C(O)OR ^A1 , -C(O)N(R ^A1 ) ₂ , -C(O)NR ^A1 (OR ^A1 ), -OC(O)R ^A1 -OC(O)N(R ^A1 ) ₂ , -OC(O)OR ^A1 , - OSO ₂ R ^A1 , -OSO ₂ N(R ^A1 ) ₂ , -N(R ^A1 )C(O)R ^A1 , -NR ^A1 C(O)OR ^A1 , -NR ^A1 C(O)N(R ^A1 ) ₂ , - N(R ^A1 )SO ₂ R ^A1 , -NR ^A1 S(O) ₂ N(R ^A1 ) ₂ , -NR ^A1 OR ^A1 , -NR ^A1 S(O)R ^A1 , -NR ^A1 S(O)N(R ^A1 ) ₂ , - S(O)R ^A1 , -SO ₂ R ^A1 , -S(O)N(R ^A1 ) ₂ , -SO ₂ N(R ^A1 ) ₂ , -SO ₃ R ^A1 , -C(=NR ^m )R ^A1 , -C(=NR ^m )N(R ^A1 ) ₂ , - NR ^A1 C(=NR ^m )R ^A1 , -NR ^A1 C(=NR ^m )N(R ^A1 ) ₂ , -NR ^A1 S(O)(=NR ^m )R ^A1 , - NR ^A1 S(O)(=NR ^m )N(R ^A1 ) ₂ , -OS(O)(=NR ^m )R ^A1 , -S(O)(=NR ^m )R ^A1 , -S(O)(=NR ^m )N(R ^A1 ) ₂ , - P(O)(R ^A1 ) ₂ , CI-6 aliphatic, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 6- membered monocyclic heteroaryl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, or 8- to 10-membered bicyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein each of the C1-6 aliphatic, 3- to 7-membered monocyclic carbocyclyl, 5- to 10-membered bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7- membered monocyclic heterocyclyl, 5- to 10-membered bicyclic heterocyclyl, 5- to 6- membered monocyclic heteroaryl, and 8- to 10-membered bicyclic heteroaryl of R ^A is independently optionally substituted with 1, 2, 3, 4, 5, or 6 R ^AG substituents;

R ¹ is hydrogen or an optionally substituted group selected from C1-6 aliphatic, 3- to 7- membered saturated or partially unsaturated monocyclic carbocyclyl, phenyl, 3- to 7- membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, and 5- to 6-membered monocyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur;

Z is N or CR ² ;

U is N or CR ⁴ ;

R ² and R ⁴ are each independently hydrogen, halogen, -CN, -OR, -SR, -N(R)2, - C(O)R, -C(O)N(R) ₂ , -C(O)NR(OR), -OC(O)R, -OC(O)N(R) ₂ , -OC(O)OR, -OSO ₂ R, - OSO ₂ N(R) ₂ , -N(R)C(O)R, -NRC(O)OR, -NRC(O)N(R) ₂ , -N(R)SO ₂ R, -NRS(O) ₂ N(R) ₂ , - NROR, -NRS(O)R, -NRS(O)N(R) ₂ , -S(O)R, -SO ₂ R, -S(O)N(R) ₂ , -SO ₂ N(R) ₂ , -SO ₃ R, - C(=NR ^m )R, -C(=NR ^m )N(R) ₂ , -NRC(=NR ^m )R, -NRC(=NR ^m )N(R) ₂ , -NRS(O)(=NR ^m )R, - NRS(O)(=NR ^m )N(R) ₂ , -OS(O)(=NR ^m )R, -S(O)(=NR ^m )R, -S(O)(=NR ^m )N(R) ₂ , -P(O)(R) ₂ , or an optionally substituted group selected from C1-6 aliphatic, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, phenyl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, and 5- to 6-membered monocyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur;

R ³ is -F, -Cl, -CN, -OR, -SR, -N(R) ₂ , -C(O)R, -C(O)N(R) ₂ , -C(O)NR(OR), -OC(O)R, -OC(O)N(R) ₂ , -OC(O)OR, -OSO ₂ R, -OSO ₂ N(R) ₂ , -N(R)C(O)R, -NRC(O)OR, - NRC(O)N(R) ₂ , -N(R)SO ₂ R, -NRS(O) ₂ N(R) ₂ , -NROR, -NRS(O)R, -NRS(O)N(R) ₂ , -S(O)R, - SO ₂ R, -S(O)N(R) ₂ , -SO ₂ N(R) ₂ , -SO ₃ R, -C(=NR ^m )R, -C(=NR ^m )N(R) ₂ , -NRC(=NR ^m )R, - NRC(=NR ^m )N(R) ₂ , -NRS(O)(=NR ^m )R, -NRS(O)(=NR ^m )N(R) ₂ , -OS(O)(=NR ^m )R, - S(O)(=NR ^m )R, -S(O)(=NR ^m )N(R) ₂ , -P(O)(R) ₂ , or an optionally substituted group selected from C1-6 aliphatic, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, phenyl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, and 5- to 6-membered monocyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur;

R ⁵ is -L ⁵ -R ^5A ;

L ⁵ is a covalent bond or optionally substituted bivalent C1-6 aliphatic;

R ^5A is -OR ^5A11 , -SR ^5A1 , -NHR ^5A12 , -N(R ^5A12 ) ₂ , -C(O)R ^5A1 , -C(O)N(R ^5A1 ) ₂ , - C(O)NR ^5A1 (OR ^5A1 ), -OC(O)R ^5A1 , -OC(O)N(R ^5A1 ) ₂ , -OC(O)OR ^5A1 , -OSO ₂ R ^5A1 , - OSO ₂ N(R ^5A1 ) ₂ , -N(R ^5A1 )C(O)R ^5A1 , -NR ^5A1 C(O)OR ^5A1 , -NR ^5A1 C(O)N(R ^5A1 ) ₂ , - N(R ^5A1 )SO ₂ R ^5A1 , -NR ^5A1 S(O) ₂ N(R ^5A1 ) ₂ , -NR ^5A1 OR ^5A1 , -NR ^5A1 S(O)R ^5A1 , -NR ^5A1 S(O)N(R ^5A1 ) ₂ , -S(O)R ^5A1 , -SO ₂ R ^5A1 , -S(O)N(R ^5A1 ) ₂ , -SO ₂ N(R ^5A1 ) ₂ , -SO ₃ R ^5A1 , -C(=NR ^m )R ^5A1 , - C(=NR ^m )N(R ^5A1 ) ₂ , -NR ^5A1 C(=NR ^m )R ^5A1 , -NR ^5A1 C(=NR ^m )N(R ^5A1 ) ₂ , -NR ^5A1 S(O)(=NR ^m )R ^5A1 , - NR ^5A1 S(O)(=NR ^m )N(R ^5A1 ) ₂ , -OS(O)(=NR ^m )R ^5A1 , -S(O)(=NR ^m )R ^5A1 , -S(O)(=NR ^m )N(R ^5A1 ) ₂ , - P(O)(R ^5A1 ) ₂ , methyl, C ₂ -6 aliphatic, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 6-membered monocyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, or 8- to 10-membered bicyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein each of the C ₂ -6 aliphatic, 3- to 7-membered monocyclic carbocyclyl, 5- to 10-membered bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7- membered monocyclic heterocyclyl, 5- to 10-membered bicyclic heterocyclyl, 5- to 6- membered monocyclic heteroaryl, and 8- to 10-membered bicyclic heteroaryl of R ^5A is independently optionally substituted with 1, 2, 3, 4, 5, or 6 R ^5AG substituents; or the methyl of R ^5A is substituted with 1, 2, or 3 R ^5AG substituents;

R ^A1 and R ^5A1 are each independently hydrogen, C1-6 aliphatic, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7- membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 6-membered monocyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, or 8- to 10-membered bicyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein each of the C1-6 aliphatic, 3- to 7-membered monocyclic carbocyclyl, 5- to 10-membered bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7- membered monocyclic heterocyclyl, 5- to 10-membered bicyclic heterocyclyl, 5- to 6- membered monocyclic heteroaryl, and 8- to 10-membered bicyclic heteroaryl of R ^A1 or R ^5A1 is independently optionally substituted with 1, 2, 3, 4, 5, or 6 R ^G1 substituents; or two R ^A1 when attached to the same nitrogen atom are taken together to form an optionally substituted ring selected from 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 0-2 additional heteroatoms independently selected from nitrogen, oxygen, and sulfur, and 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 0-3 additional heteroatoms independently selected from nitrogen, oxygen, and sulfur; or two R ^5A1 when attached to the same nitrogen atom are taken together to form an optionally substituted ring selected from 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 0-2 additional heteroatoms independently selected from nitrogen, oxygen, and sulfur, and 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 0-3 additional heteroatoms independently selected from nitrogen, oxygen, and sulfur;

R ^5A11 is methyl, C2-6 aliphatic, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 6-membered monocyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, or 8- to 10-membered bicyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein each of the C2-6 aliphatic, 3- to 7-membered monocyclic carbocyclyl, 5- to 10-membered bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7- membered monocyclic heterocyclyl, 5- to 10-membered bicyclic heterocyclyl, 5- to 6- membered monocyclic heteroaryl, and 8- to 10-membered bicyclic heteroaryl of R ^5A11 is independently optionally substituted with 1, 2, 3, 4, 5, or 6 R ^G1 substituents; or the methyl of R ^5A11 is substituted with 1, 2, or 3 R ^G1 substituents; each R ^5A12 is independently C1-6 aliphatic, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1-3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 6-membered monocyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, or 8- to 10-membered bicyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein each of the C1-6 aliphatic, 3- to 7-membered monocyclic carbocyclyl, 5- to 10-membered bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7- membered monocyclic heterocyclyl, 5- to 10-membered bicyclic heterocyclyl, 5- to 6- membered monocyclic heteroaryl, and 8- to 10-membered bicyclic heteroaryl of R ^5A12 is independently optionally substituted with 1, 2, 3, 4, 5, or 6 R ^G1 substituents; or two R ^5A12 when attached to the same nitrogen atom are taken together to form an optionally substituted ring selected from 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 0-2 additional heteroatoms independently selected from nitrogen, oxygen, and sulfur, and 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 0-3 additional heteroatoms independently selected from nitrogen, oxygen, and sulfur;

R ^AG , R ^5AG , and R ^G1 are each independently halogen, -CN, -OR, -SR, -N(R)2, -C(O)R, -C(O)OR, -C(O)N(R) ₂ , -C(O)NR(OR), -OC(O)R, -OC(O)N(R) ₂ , -OC(O)OR, -OSO ₂ R, - OSO ₂ N(R) ₂ , -N(R)C(O)R, -NRC(O)OR, -NRC(O)N(R) ₂ , -N(R)SO ₂ R, -NRS(O) ₂ N(R) ₂ , - NROR, -NRS(O)R, -NRS(O)N(R) ₂ , -S(O)R, -SO ₂ R, -S(O)N(R) ₂ , -SO ₂ N(R) ₂ , -SO ₃ R, - C(=NR ^m )R, -C(=NR ^m )N(R) ₂ , -NRC(=NR ^m )R, -NRC(=NR ^m )N(R) ₂ , -NRS(O)(=NR ^m )R, - NRS(O)(=NR ^m )N(R) ₂ , -OS(O)(=NR ^m )R, -S(O)(=NR ^m )R, -S(O)(=NR ^m )N(R) ₂ , -P(O)(R) ₂ , or an optionally substituted group selected from C1-6 aliphatic, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 6-membered monocyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, and 8- to 10- membered bicyclic heteroaryl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur; each R is independently hydrogen or an optionally substituted group selected from Ci- 6 aliphatic, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic carbocyclyl, phenyl, 8- to 10- membered bicyclic aryl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 6-membered monocyclic heteroaryl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, and 8- to 10-membered bicyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur; or two R when attached to the same nitrogen atom are taken together to form an optionally substituted ring selected from 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 0-2 additional heteroatoms independently selected from nitrogen, oxygen, and sulfur, and 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 0-3 additional heteroatoms independently selected from nitrogen, oxygen, and sulfur; each R ^m is independently -OH, -CN, or R; and n is 0, 1, 2, 3, or 4.

In some embodiments:

- is a single or double bond;

X is N or C;

Y is N or C;

Ring A is phenyl, or 5- to 6-membered monocyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur; each L ^A is independently a covalent bond or a bivalent C1-6 aliphatic, wherein the bivalent C1-6 aliphatic is optionally substituted with 1, 2, 3, or 4 independently selected R ^LA1 substituents; each R ^A is independently oxo, halogen, -CN, -OR ^A1 , -SR ^A1 , -N(R ^A1 )2, -C(O)R ^A1 , -C(O)OR ^A1 , -C(O)N(R ^A1 ) ₂ , -C(O)NR ^A1 (OR ^A1 ), -OC(O)R ^A1 -OC(O)N(R ^A1 ) ₂ , -OC(O)OR ^A1 , - OSO ₂ R ^A1 , -OSO ₂ N(R ^A1 ) ₂ , -N(R ^A1 )C(O)R ^A1 , -NR ^A1 C(O)OR ^A1 , -NR ^A1 C(O)N(R ^A1 ) ₂ , - N(R ^A1 )SO ₂ R ^A1 , -NR ^A1 S(O) ₂ N(R ^A1 ) ₂ , -NR ^A1 OR ^A1 , -NR ^A1 S(O)R ^A1 , -NR ^A1 S(O)N(R ^A1 ) ₂ , - S(O)R ^A1 , -SO ₂ R ^A1 , -S(O)N(R ^A1 ) ₂ , -SO ₂ N(R ^A1 ) ₂ , -SO ₃ R ^A1 , -C(=NR ^m )R ^A1 , -C(=NR ^m )N(R ^A1 ) ₂ , - NR ^A1 C(=NR ^m )R ^A1 , -NR ^A1 C(=NR ^m )N(R ^A1 ) ₂ , -NR ^A1 S(O)(=NR ^m )R ^A1 , - NR ^A1 S(O)(=NR ^m )N(R ^A1 ) ₂ , -OS(O)(=NR ^m )R ^A1 , -S(O)(=NR ^m )R ^A1 , -S(O)(=NR ^m )N(R ^A1 ) ₂ , - P(O)(R ^A1 ) ₂ , CI-6 aliphatic, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 6- membered monocyclic heteroaryl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, or 8- to 10-membered bicyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein each of the C1-6 aliphatic, 3- to 7-membered monocyclic carbocyclyl, 5- to 10-membered bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7- membered monocyclic heterocyclyl, 5- to 10-membered bicyclic heterocyclyl, 5- to 6- membered monocyclic heteroaryl, and 8- to 10-membered bicyclic heteroaryl of R ^A is independently optionally substituted with 1, 2, 3, 4, 5, or 6 R ^AG substituents;

R ¹ is hydrogen or a group selected from C1-6 aliphatic, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, phenyl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, and 5- to 6-membered monocyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein the Ci-6 aliphatic, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, phenyl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl, and 5- to 6-membered monocyclic heteroaryl are each optionally substituted with 1, 2, 3, or 4 independently selected R ^1A substituents;

Z is N or CR ² ;

U is N or CR ⁴ ;

R ² and R ⁴ are each independently hydrogen, halogen, -CN, -OR, -SR, -N(R) ₂ , - C(O)R, -C(O)N(R) ₂ , -C(O)NR(OR), -OC(O)R, -OC(O)N(R) ₂ , -OC(O)OR, -OSO ₂ R, - OSO ₂ N(R) ₂ , -N(R)C(O)R, -NRC(O)OR, -NRC(O)N(R) ₂ , -N(R)SO ₂ R, -NRS(O) ₂ N(R) ₂ , - NROR, -NRS(O)R, -NRS(O)N(R) ₂ , -S(O)R, -SO ₂ R, -S(O)N(R) ₂ , -SO ₂ N(R) ₂ , -SO ₃ R, - C(=NR ^m )R, -C(=NR ^m )N(R) ₂ , -NRC(=NR ^m )R, -NRC(=NR ^m )N(R) ₂ , -NRS(O)(=NR ^m )R, - NRS(O)(=NR ^m )N(R) ₂ , -OS(O)(=NR ^m )R, -S(O)(=NR ^m )R, -S(O)(=NR ^m )N(R) ₂ , -P(O)(R) ₂ , Ci- ₆ aliphatic, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, phenyl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1-3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, and 5- to 6- membered monocyclic heteroaryl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein the C1-6 aliphatic, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, phenyl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl, and 5- to 6-membered monocyclic heteroaryl are each optionally substituted with 1, 2, 3, or 4 independently selected R ^2A substituents;

R ³ is -F, -Cl, -CN, -OR, -SR, -N(R) ₂ , -C(O)R, -C(O)N(R) ₂ , -C(O)NR(OR), -OC(O)R, -OC(O)N(R) ₂ , -OC(O)OR, -OSO ₂ R, -OSO ₂ N(R) ₂ , -N(R)C(O)R, -NRC(O)OR, - NRC(O)N(R) ₂ , -N(R)SO ₂ R, -NRS(O) ₂ N(R) ₂ , -NROR, -NRS(O)R, -NRS(O)N(R) ₂ , -S(O)R, - SO ₂ R, -S(O)N(R) ₂ , -SO ₂ N(R) ₂ , -SO ₃ R, -C(=NR ^m )R, -C(=NR ^m )N(R) ₂ , -NRC(=NR ^m )R, - NRC(=NR ^m )N(R) ₂ , -NRS(O)(=NR ^m )R, -NRS(O)(=NR ^m )N(R) ₂ , -OS(O)(=NR ^m )R, - S(O)(=NR ^m )R, -S(O)(=NR ^m )N(R) ₂ , -P(O)(R) ₂ , C1-6 aliphatic, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, phenyl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, and 5- to 6-membered monocyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein the Ci-6 aliphatic, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, phenyl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl, and 5- to 6-membered monocyclic heteroaryl are each optionally substituted with 1, 2, 3, or 4 independently selected R ^3A substituents;

R ⁵ is -L ⁵ -R ^5A ;

L ⁵ is a covalent bond or a bivalent C1-6 aliphatic, wherein the bivalent C1-6 aliphatic is optionally substituted with 1, 2, 3, or 4 independently selected R ^L5A substituents;

R ^5A is -OR ^5A11 , -SR ^5A1 , -NHR ^5A12 , -N(R ^5A12 ) ₂ , -C(O)R ^5A1 , -C(O)N(R ^5A1 ) ₂ , - C(O)NR ^5A1 (OR ^5A1 ), -OC(O)R ^5A1 , -OC(O)N(R ^5A1 ) ₂ , -OC(O)OR ^5A1 , -OSO ₂ R ^5A1 , - OSO ₂ N(R ^5A1 ) ₂ , -N(R ^5A1 )C(O)R ^5A1 , -NR ^5A1 C(O)OR ^5A1 , -NR ^5A1 C(O)N(R ^5A1 ) ₂ , - N(R ^5A1 )SO ₂ R ^5A1 , -NR ^5A1 S(O) ₂ N(R ^5A1 ) ₂ , -NR ^5A1 OR ^5A1 , -NR ^5A1 S(O)R ^5A1 , -NR ^5A1 S(O)N(R ^5A1 ) ₂ , -S(O)R ^5A1 , -SO ₂ R ^5A1 , -S(O)N(R ^5A1 ) ₂ , -SO ₂ N(R ^5A1 ) ₂ , -SO ₃ R ^5A1 , -C(=NR ^m )R ^5A1 , - C(=NR ^m )N(R ^5A1 ) ₂ , -NR ^5A1 C(=NR ^m )R ^5A1 , -NR ^5A1 C(=NR ^m )N(R ^5A1 ) ₂ , -NR ^5A1 S(O)(=NR ^m )R ^5A1 , - NR ^5A1 S(O)(=NR ^m )N(R ^5A1 ) ₂ , -OS(O)(=NR ^m )R ^5A1 , -S(O)(=NR ^m )R ^5A1 , -S(O)(=NR ^m )N(R ^5A1 ) ₂ , - P(O)(R ^5A1 ) ₂ , methyl, C ₂ -6 aliphatic, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 6-membered monocyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, or 8- to 10-membered bicyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein each of the C2-6 aliphatic, 3- to 7-membered monocyclic carbocyclyl, 5- to 10-membered bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7- membered monocyclic heterocyclyl, 5- to 10-membered bicyclic heterocyclyl, 5- to 6- membered monocyclic heteroaryl, and 8- to 10-membered bicyclic heteroaryl of R ^5A is independently optionally substituted with 1, 2, 3, 4, 5, or 6 R ^5AG substituents; or the methyl of R ^5A is substituted with 1, 2, or 3 R ^5AG substituents;

R ^A1 and R ^5A1 are each independently hydrogen, C1-6 aliphatic, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7- membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 6-membered monocyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, or 8- to 10-membered bicyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein each of the C1-6 aliphatic, 3- to 7-membered monocyclic carbocyclyl, 5- to 10-membered bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7- membered monocyclic heterocyclyl, 5- to 10-membered bicyclic heterocyclyl, 5- to 6- membered monocyclic heteroaryl, and 8- to 10-membered bicyclic heteroaryl of R ^A1 or R ^5A1 is independently optionally substituted with 1, 2, 3, 4, 5, or 6 R ^G1 substituents; or two R ^A1 when attached to the same nitrogen atom are taken together to form a ring selected from 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 0-2 additional heteroatoms independently selected from nitrogen, oxygen, and sulfur, and 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 0-3 additional heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein the 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl and 5- to 10- membered saturated or partially unsaturated bicyclic heterocyclyl are each optionally substituted with 1, 2, 3, or 4 independently selected R ^A11 substituents; or two R ^5A1 when attached to the same nitrogen atom are taken together to form a ring selected from 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 0-2 additional heteroatoms independently selected from nitrogen, oxygen, and sulfur, and 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 0-3 additional heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein the 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl and 5- to 10- membered saturated or partially unsaturated bicyclic heterocyclyl are each optionally substituted with 1, 2, 3, or 4 independently selected R ^A11 substituents;

R ^5A11 is methyl, C2-6 aliphatic, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 6-membered monocyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, or 8- to 10-membered bicyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein each of the C2-6 aliphatic, 3- to 7-membered monocyclic carbocyclyl, 5- to 10-membered bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7- membered monocyclic heterocyclyl, 5- to 10-membered bicyclic heterocyclyl, 5- to 6- membered monocyclic heteroaryl, and 8- to 10-membered bicyclic heteroaryl of R ^5A11 is independently optionally substituted with 1, 2, 3, 4, 5, or 6 R ^G1 substituents; or the methyl of R ^5A11 is substituted with 1, 2, or 3 R ^G1 substituents; each R ^5A12 is independently C1-6 aliphatic, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1-3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 6-membered monocyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, or 8- to 10-membered bicyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein each of the C1-6 aliphatic, 3- to 7-membered monocyclic carbocyclyl, 5- to 10-membered bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7- membered monocyclic heterocyclyl, 5- to 10-membered bicyclic heterocyclyl, 5- to 6- membered monocyclic heteroaryl, and 8- to 10-membered bicyclic heteroaryl of R ^5A12 is independently optionally substituted with 1, 2, 3, 4, 5, or 6 R ^G1 substituents; or two R ^5A12 when attached to the same nitrogen atom are taken together to form a ring selected from 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 0-2 additional heteroatoms independently selected from nitrogen, oxygen, and sulfur, and 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 0-3 additional heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein the 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl and 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl are each optionally substituted with 1, 2, 3, or 4 independently selected R ^5B12 substituents;

R ^AG , R ^5AG , and R ^G1 are each independently halogen, -CN, -OR, -SR, -N(R)2, -C(O)R, -C(O)OR, -C(O)N(R) ₂ , -C(O)NR(OR), -OC(O)R, -OC(O)N(R) ₂ , -OC(O)OR, -OSO ₂ R, - OSO ₂ N(R) ₂ , -N(R)C(O)R, -NRC(O)OR, -NRC(O)N(R) ₂ , -N(R)SO ₂ R, -NRS(O) ₂ N(R) ₂ , - NROR, -NRS(O)R, -NRS(O)N(R) ₂ , -S(O)R, -SO ₂ R, -S(O)N(R) ₂ , -SO ₂ N(R) ₂ , -SO ₃ R, - C(=NR ^m )R, -C(=NR ^m )N(R) ₂ , -NRC(=NR ^m )R, -NRC(=NR ^m )N(R) ₂ , -NRS(O)(=NR ^m )R, - NRS(O)(=NR ^m )N(R) ₂ , -OS(O)(=NR ^m )R, -S(O)(=NR ^m )R, -S(O)(=NR ^m )N(R) ₂ , -P(O)(R) ₂ , Ci- ₆ aliphatic, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic carbocyclyl, phenyl, 8- to 10- membered bicyclic aryl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 6-membered monocyclic heteroaryl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, and 8- to 10-membered bicyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein the C1-6 aliphatic, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7- membered saturated or partially unsaturated monocyclic heterocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl, 5- to 6-membered monocyclic heteroaryl, and 8- to 10-membered bicyclic heteroaryl are each optionally substituted with 1, 2, 3, or 4 independently selected R ^AG1 substituents; each R is independently selected from hydrogen, C1-6 aliphatic, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7- membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 6-membered monocyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, and 8- to 10- membered bicyclic heteroaryl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein the C1-6 aliphatic, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl, 5- to 6-membered monocyclic heteroaryl, and 8- to 10- membered bicyclic heteroaryl are each optionally substituted with 1, 2, 3, or 4 independently selected R ^N substituents; or two R when attached to the same nitrogen atom are taken together to form a ring selected from 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 0-2 additional heteroatoms independently selected from nitrogen, oxygen, and sulfur, and 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 0-3 additional heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein the 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl and 5- to 10- membered saturated or partially unsaturated bicyclic heterocyclyl are each optionally substituted with 1, 2, 3, or 4 independently selected R ^N substituents; each R ^LA1 , R ^1A , R ^2A , R ^3A , R ^L5A , R ^A11 , R ^5B12 , R ^AG1 , and R ^N is independently selected from halogen, -(CH ₂ ) ₀ ^R°, -(CH ₂ ) ₀ ^OR°, -O(CH ₂ ) _0.4 R°, -O-(CH ₂ ) _0-4 C(O)OR°, -(CH ₂ ) ₀ _ ₄ CH(OR°) ₂ , -(CH ₂ ) _O-4 SR°, -(CH ₂ ) ₀ ^Ph, -(CH ₂ )(I- ₄ O(CH ₂ )(I-I Ph. -CH=CHPh, -(CH ₂ ) ₀ _ ₄ 0(CH ₂ )o-i-pyridyl, -NO ₂ , -CN, -N ₃ , -(CH ₂ ) _0-4 N(R°) ₂ , -(CH ₂ ) _0-4 N(R°)C(O)R°, - N(R°)C(S)R°, -(CH ₂ )O^N(R°)C(0)NR° ₂ , -N(R ^O )C(S)NR° ₂ , -(CH ₂ ) ₀ ^N(R ^O )C(O)OR°, - N(R°)N(R°)C(O)R°, -N(R°)N(R°)C(O)NR° ₂ , -N(R°)N(R°)C(O)OR°, -(CH ₂ ) _0-4 C(O)R ^O , - C(S)R°, -(CH ₂ ) _0-4 C(O)OR°, -(CH ₂ )O^C(0)SR°, -(CH ₂ ) ₀ ^C(O)OSIR°3, -(CH ₂ ) _0-4 OC(O)R ^O , - OC(0)(CH ₂ )O_ ₄ SR°, -(CH ₂ )O^SC(0)R°, -(CH ₂ ) _0-4 C(O)NR ^O ₂ , -C(S)NR ^O ₂ , -C(S)SR°, - SC(S)SR°, -(CH ₂ ) ₀ ^OC(O)NR° ₂ , -C(O)N(OR°)R°, -C(O)C(O)R°, -C(O)CH ₂ C(O)R°, - C(NOR°)R°, -(CH ₂ )O^SSR°, -(CH ₂ )O^S(0) ₂ R°, -(CH ₂ ) _0-4 S(O)(=NR ^O )R°, -(CH ₂ ) _O _ ₄ S(0) ₂ 0R ^O , -(CH ₂ ) _0-4 OS(O) ₂ R ^O , -(CH ₂ )O. ₄ -S(0) ₂ NR° ₂ , -(CH ₂ ) _0.4 S(O)(=NR ^O )NR° ₂ , -(CH ₂ ) _O _ ₄ S(O)R°, -N(R°)S(0) ₂ NR ^O ₂ , -N(R°)S(O) ₂ R°, -N(R°)S(O)(=NR°)R°, -N(OR°)R°, - C(NH)NR° ₂ , -P(O) ₂ R°, -P(O)R° ₂ , -OP(O)R° ₂ , -OP(O)(OR°) ₂ , -SiR°3, -(Ci- ₄ straight or branched alkylene)O-N(R°) ₂ , and — (Ci- ₄ straight or branched alkylene)C(O)O-N(R°) ₂ ; each R° is independently hydrogen, Ci-6 aliphatic, -C FbPh. -0(CH2)o-iPh, -CH2-(5- to 6-membered heteroaryl ring), or a 3 - to 6-membered saturated, partially unsaturated, or aryl ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur; or two independent occurrences of R°, taken together with their intervening atoms, form a 3 - to 12-membered saturated, partially unsaturated, or aryl mono- or bicyclic ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur; each R ^m is independently -OH, -CN, or R; and n is 0, 1, 2, 3, or 4.

In some embodiments, the present disclosure provides compounds of Formula II:

II or a pharmaceutically acceptable salt thereof, wherein each of X, Y, Z, U, Ring A, L ^A , R ^A , R ¹ , R ³ , L ⁵ , R ^5A12 , and n is defined above for Formula I and described in classes and subclasses herein, both singly and in combination.

In some embodiments, the present disclosure provides compounds of Formula III:

III or a pharmaceutically acceptable salt thereof, wherein each of Z, U, Ring A, L ^A , R ^A , R ¹ , R ³ , L ⁵ , R ^5A12 , and n is defined above for Formula I and described in classes and subclasses herein, both singly and in combination.

As described above, in some embodiments of any of Formulae I and II, is a single or double bond. In some embodiments, - is a single bond. In some embodiments,

- is a double bond. As described above, in some embodiments of any of Formulae I and II, X is N or C. In some embodiments, X is N. In some embodiments, X is C.

As described above, in some embodiments of any of Formulae I and II, Y is N or C. In some embodiments, Y is N. In some embodiments, Y is C.

In some embodiments, X is C and Y is N. In some embodiments, X is N and Y is C. In some embodiments, X is C and Y is C.

As described above, in some embodiments of any of Formulae I, II, and III, Ring A is phenyl, or 5- to 6-membered monocyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur. In some embodiments of any of Formulae I, II, III, IV, V, and Va, Ring A is phenyl, or 5- to 6-membered monocyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur.

In some embodiments, Ring A is 5- to 6-membered monocyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur. In some embodiments, Ring A is 5 -membered monocyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur. In some embodiments, Ring A is 5 -membered monocyclic heteroaryl having 1-3 nitrogen atoms. In some embodiments, Ring A is 5 -membered monocyclic heteroaryl having 1-2 nitrogen atoms. In some embodiments, Ring A is 6- membered monocyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur. In some embodiments, Ring A is 6-membered monocyclic heteroaryl having 1-3 nitrogen atoms. In some embodiments, Ring A is 6-membered monocyclic heteroaryl having 1-2 nitrogen atoms. In some embodiments, Ring A is selected from pyrazolyl, imidazolyl, triazolyl, and pyridyl. In some embodiments, Ring A is pyrazolyl. In some embodiments, Ring A is imidazolyl. In some embodiments, Ring A is triazolyl. In some embodiments, Ring A is pyridyl.

As described above, in some embodiments of any of Formulae I, II, and III, each L ^A is independently a covalent bond or optionally substituted bivalent C1-6 aliphatic. In some embodiments of any of Formulae I, II, III, and IV, each L ^A is independently a covalent bond or optionally substituted bivalent C1-6 aliphatic. In some embodiments, L ^A is a covalent bond or a bivalent C1-2 aliphatic (e.g., -CH2- or -CH2CH2-). In some embodiments, L ^A is a covalent bond. In some embodiments, L ^A is a bivalent C1-6 aliphatic. In some embodiments, L ^A is a bivalent C1-3 aliphatic. In some embodiments, L ^A is a bivalent C1-2 aliphatic (e.g., -CH2- or - CH2CH2-). In some embodiments, L ^A is a bivalent Ci aliphatic (e.g., -CH2-). In some embodiments, L ^A is a bivalent C2 aliphatic (e.g., -CH2CH2-).

In some embodiments of any of Formulae I, II, III, and IV, each R ^A is independently oxo, halogen, -CN, -OR ^A1 , -SR ^A1 , -N(R ^A1 ) ₂ , -C(O)R ^A1 , -C(O)OR ^A1 , -C(O)N(R ^A1 ) ₂ , - C(O)NR ^A1 (OR ^A1 ), -OC(O)R ^A1 -OC(O)N(R ^A1 ) ₂ , -OC(O)OR ^A1 , -OSO ₂ R ^A1 , -OSO ₂ N(R ^A1 ) ₂ , - N(R ^A1 )C(O)R ^A1 , -NR ^A1 C(O)OR ^A1 , -NR ^A1 C(O)N(R ^A1 ) ₂ , -N(R ^A1 )SO ₂ R ^A1 , -NR ^A1 S(O) ₂ N(R ^A1 ) ₂ , - NR ^A1 OR ^A1 , -NR ^A1 S(O)R ^A1 , -NR ^A1 S(O)N(R ^A1 ) ₂ , -S(O)R ^A1 , -SO ₂ R ^A1 , -S(O)N(R ^A1 ) ₂ , - SO ₂ N(R ^A1 ) ₂ , -SO ₃ R ^A1 , -C(=NR ^m )R ^A1 , -C(=NR ^m )N(R ^A1 ) ₂ , -NR ^A1 C(=NR ^m )R ^A1 , - NR ^A1 C(=NR ^m )N(R ^A1 ) ₂ , -NR ^A1 S(O)(=NR ^m )R ^A1 , -NR ^A1 S(O)(=NR ^m )N(R ^A1 ) ₂ , - OS(O)(=NR ^m )R ^A1 , -S(O)(=NR ^m )R ^A1 , -S(O)(=NR ^m )N(R ^A1 ) ₂ , -P(O)(R ^A1 ) ₂ , Ci- ₆ aliphatic, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1-3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5 - to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 6-membered monocyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, or 8- to 10- membered bicyclic heteroaryl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein each of the C1-6 aliphatic, 3- to 7-membered monocyclic carbocyclyl, 5- to 10-membered bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7-membered monocyclic heterocyclyl, 5- to 10-membered bicyclic heterocyclyl, 5- to 6-membered monocyclic heteroaryl, and 8- to 10-membered bicyclic heteroaryl of R ^A is independently optionally substituted with 1, 2, 3, 4, 5, or 6 R ^AG substituents.

As described above, in some embodiments of any of Formulae I, II, and III, each R ^A is independently oxo, halogen, -CN, -OR ^A1 , -SR ^A1 , -N(R ^A1 ) ₂ , -C(O)R ^A1 , -C(O)OR ^A1 , - C(O)N(R ^A1 ) ₂ , -C(O)NR ^A1 (OR ^A1 ), -OC(O)R ^A1 -OC(O)N(R ^A1 ) ₂ , -OC(O)OR ^A1 , -OSO ₂ R ^A1 , - OSO ₂ N(R ^A1 ) ₂ , -N(R ^A1 )C(O)R ^A1 , -NR ^A1 C(O)OR ^A1 , -NR ^A1 C(O)N(R ^A1 ) ₂ , -N(R ^A1 )SO ₂ R ^A1 , - NR ^A1 S(O) ₂ N(R ^A1 ) ₂ , -NR ^A1 OR ^A1 , -NR ^A1 S(O)R ^A1 , -NR ^A1 S(O)N(R ^A1 ) ₂ , -S(O)R ^A1 , -SO ₂ R ^A1 , - S(O)N(R ^A1 ) ₂ , -SO ₂ N(R ^A1 ) ₂ , -SO ₃ R ^A1 , -C(=NR ^m )R ^A1 , -C(=NR ^m )N(R ^A1 ) ₂ , -NR ^A1 C(=NR ^m )R ^A1 , - NR ^A1 C(=NR ^m )N(R ^A1 ) ₂ , -NR ^A1 S(O)(=NR ^m )R ^A1 , -NR ^A1 S(O)(=NR ^m )N(R ^A1 ) ₂ , - OS(O)(=NR ^m )R ^A1 , -S(O)(=NR ^m )R ^A1 , -S(O)(=NR ^m )N(R ^A1 ) ₂ , -P(O)(R ^A1 ) ₂ , Ci- ₆ aliphatic, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1-3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5 - to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 6-membered monocyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, or 8- to 10- membered bicyclic heteroaryl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein each of the C1-6 aliphatic, 3- to 7-membered monocyclic carbocyclyl, 5- to 10-membered bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7-membered monocyclic heterocyclyl, 5- to 10-membered bicyclic heterocyclyl, 5- to 6-membered monocyclic heteroaryl, and 8- to 10-membered bicyclic heteroaryl of R ^A is independently optionally substituted with 1, 2, 3, 4, 5, or 6 R ^AG substituents.

In some embodiments, each R ^A is independently C1-6 aliphatic, -OR ^A1 , -N(R ^A1 )2, - to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1-3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5 - to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 6-membered monocyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, or 8- to 10- membered bicyclic heteroaryl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur; wherein each of the C1-6 aliphatic, 3- to 7-membered monocyclic carbocyclyl, 5- to 10-membered bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7-membered monocyclic heterocyclyl, 5- to 10-membered bicyclic heterocyclyl, 5- to 6-membered monocyclic heteroaryl, and 8- to 10-membered bicyclic heteroaryl of R ^A is independently optionally substituted with 1, 2, 3, 4, 5, or 6 R ^AG substituents.

In some embodiments, each R ^A is independently C1-6 alkyl, C2-6 alkenyl, -OR ^A1 , - N(R ^A1 ) ₂ , -C(O)R ^A1 , -C(0)N(R ^A1 )2, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, phenyl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, or 5- to 6-membered monocyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein the C1-6 alkyl, C2-6 alkenyl, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, phenyl, 3- to 7- membered saturated or partially unsaturated monocyclic heterocyclyl, and 5- to 6-membered monocyclic heteroaryl of R ^A are each independently optionally substituted with 1, 2, 3, 4, 5, or 6 R ^AG substituents.

In some embodiments, each R ^A is independently C1-6 alkyl, C2-6 alkenyl, -OR ^A1 , - N(R ^A1 ) ₂ , -C(O)R ^A1 , -C(0)N(R ^A1 )2, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, phenyl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, or 5- to 6-membered monocyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein the C1-6 alkyl, C2-6 alkenyl, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, phenyl, 3- to 7- membered saturated or partially unsaturated monocyclic heterocyclyl, and 5- to 6-membered monocyclic heteroaryl of R ^A are each independently optionally substituted with 1, 2, 3, or 4 R ^AG substituents.

In some embodiments, each R ^A is independently C1-6 alkyl, C2-6 alkenyl, -OR ^A1 , - N(R ^A1 ) ₂ , -C(O)R ^A1 , -C(O)N(R ^A1 ) ₂ , 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, phenyl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, or 5- to 6-membered monocyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein the C1-6 alkyl, C ₂ -6 alkenyl, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, phenyl, 3- to 7- membered saturated or partially unsaturated monocyclic heterocyclyl, and 5- to 6-membered monocyclic heteroaryl of R ^A are each independently optionally substituted with 1 or 2 R ^AG substituents.

In some embodiments, each R ^A is independently C1-6 alkyl, C ₂ -6 alkenyl, -OR ^A1 , - N(R ^A1 ) ₂ , 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic carbocyclyl, phenyl, 8- to 10- membered bicyclic aryl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 6-membered monocyclic heteroaryl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, or 8- to 10-membered bicyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur; wherein each of the C1-6 alkyl, C ₂ -6 alkenyl, 3- to 7-membered monocyclic carbocyclyl, 5- to 10-membered bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7-membered monocyclic heterocyclyl, 5- to 10-membered bicyclic heterocyclyl, 5- to 6-membered monocyclic heteroaryl, and 8- to 10-membered bicyclic heteroaryl of R ^A is independently optionally substituted with 1, 2, 3, 4, 5, or 6 R ^AG substituents.

In some embodiments, each R ^A is independently C1-6 aliphatic, -OR ^A1 , -N(R ^A1 ) ₂ , 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1-3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5 - to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 6-membered monocyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, or 8- to 10- membered bicyclic heteroaryl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur; wherein each of the C1-6 aliphatic, 3- to 7-membered monocyclic carbocyclyl, 5- to 10-membered bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7-membered monocyclic heterocyclyl, 5- to 10-membered bicyclic heterocyclyl, 5- to 6-membered monocyclic heteroaryl, and 8- to 10-membered bicyclic heteroaryl of R ^A are each optionally substituted with 1, 2, 3, 4, 5, or 6 independently selected R ^AG substituents.

In some embodiments, each R ^A is independently C1-6 alkyl, C2-6 alkenyl, -OR ^A1 , - N(R ^A1 ) ₂ , 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic carbocyclyl, phenyl, 8- to 10- membered bicyclic aryl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 6-membered monocyclic heteroaryl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, or 8- to 10-membered bicyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur; wherein each of the C1-6 alkyl, C2-6 alkenyl, 3- to 7-membered monocyclic carbocyclyl, 5- to 10-membered bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7-membered monocyclic heterocyclyl, 5- to 10-membered bicyclic heterocyclyl, 5- to 6-membered monocyclic heteroaryl, and 8- to 10-membered bicyclic heteroaryl of R ^A are each optionally substituted with 1, 2, 3, or 4 independently selected R ^AG substituents.

In some embodiments, each R ^A is independently C1-6 alkyl, C2-6 alkenyl, -OR ^A1 , - N(R ^{A I} ) ₂ . 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic carbocyclyl, phenyl, 8- to 10- membered bicyclic aryl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 6-membered monocyclic heteroaryl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, or 8- to 10-membered bicyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur; wherein each of the C1-6 alkyl, C2-6 alkenyl, 3- to 7-membered monocyclic carbocyclyl, 5- to 10-membered bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7-membered monocyclic heterocyclyl, 5- to 10-membered bicyclic heterocyclyl, 5- to 6-membered monocyclic heteroaryl, and 8- to 10-membered bicyclic heteroaryl of R ^A are each optionally substituted with 1 or 2 independently selected R ^AG substituents.

In some embodiments, each R ^A is independently C1-6 alkyl, C2-6 alkenyl, -OR ^A1 , - N(R ^A1 ) ₂ , 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, phenyl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1-3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, or 5- to 6-membered monocyclic heteroaryl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein the C1-6 alkyl, C2-6 alkenyl, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, phenyl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl, and 5- to 6-membered monocyclic heteroaryl of R ^A are each independently optionally substituted with 1 or 2 R ^AG substituents.

In some embodiments, each R ^A is independently C1-6 alkyl, C2-6 alkenyl, -OR ^A1 , - N(R ^{A I} ) ₂ . phenyl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1-3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, or 5- to 6- membered monocyclic heteroaryl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein the C1-6 alkyl, C2-6 alkenyl, phenyl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl, and 5- to 6-membered monocyclic heteroaryl of R ^A are each independently optionally substituted with 1 or 2 R ^AG substituents.

In some embodiments, each R ^A is independently C1-6 alkyl, C2-6 alkenyl, -OR ^A1 , - N(R ^{A I} ) ₂ . phenyl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1-3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, or 5- to 6- membered monocyclic heteroaryl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein the C1-6 alkyl, C2-6 alkenyl, phenyl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl, and 5- to 6-membered monocyclic heteroaryl of R ^A are each independently optionally substituted with 1 or 2 R ^AG substituents.

In some embodiments, each R ^A is independently selected from phenyl, 3- to 7- membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, and 5- to 6-membered monocyclic heteroaryl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, each of which is optionally substituted with 1, 2, 3, 4, 5, or 6 independently selected R ^AG substituents.

In some embodiments, each R ^A is independently selected from phenyl, 3- to 7- membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, and 5- to 6-membered monocyclic heteroaryl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, each of which is optionally substituted with 1 or 2 independently selected R ^AG substituents.

In some embodiments, each R ^A is independently selected from 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1-3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, and 5- to 6-membered monocyclic heteroaryl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, each of which is optionally substituted with 1 or 2 independently selected R ^AG substituents.

In some embodiments, each R ^A is independently selected from 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, each of which is optionally substituted with 1 or 2 independently selected R ^AG substituents.

In some embodiments, each R ^A is independently selected from 5- to 6-membered monocyclic heteroaryl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, each of which is optionally substituted with 1 or 2 independently selected R ^AG substituents.

In some embodiments, R ^A is phenyl optionally substituted with 1, 2, 3, 4, 5, or 6 R ^AG substituents. In some embodiments, R ^A is phenyl.

In some embodiments, R ^A is C1-6 aliphatic optionally substituted with 1, 2, 3, 4, 5, or 6 R ^AG substituents. In some embodiments, R ^A is C1-3 aliphatic optionally substituted with 1, 2, 3, 4, 5, or 6 R ^AG substituents. In some embodiments, R ^A is C1-2 aliphatic optionally substituted with 1, 2, 3, 4, or 5 R ^AG substituents. In some embodiments, R ^A is C1-2 aliphatic optionally substituted with 1, 2, 3, 4, or 5 R ^AG substituents. In some embodiments, R ^A is C2 alkenyl optionally substituted with 1, 2, or 3 R ^AG substituents. In some embodiments, R ^A is - CH=CH ₂ .

In some embodiments, two L ^A -R ^A taken together with the atoms to which they are attached form a 3 - to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic carbocyclyl, phenyl, 8- to 10- membered bicyclic aryl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 6-membered monocyclic heteroaryl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, or 8- to 10-membered bicyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein each of the 3- to 7-membered monocyclic carbocyclyl, 5- to 10-membered bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7-membered monocyclic heterocyclyl, 5- to 10-membered bicyclic heterocyclyl, 5- to 6-membered monocyclic heteroaryl, and 8- to 10-membered bicyclic heteroaryl is independently optionally substituted with 1, 2, 3, 4, 5, or 6 R ^AG substituents.

In some embodiments, each R ^A1 is independently hydrogen, C1-6 aliphatic, 3- to 7- membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1-3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5 - to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 6-membered monocyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, or 8- to 10- membered bicyclic heteroaryl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein each of the C1-6 aliphatic, 3- to 7-membered monocyclic carbocyclyl, 5- to 10-membered bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7-membered monocyclic heterocyclyl, 5- to 10-membered bicyclic heterocyclyl, 5- to 6-membered monocyclic heteroaryl, and 8- to 10-membered bicyclic heteroaryl of R ^A1 is independently optionally substituted with 1, 2, 3, 4, 5, or 6 R ^G1 substituents; or two R ^A1 when attached to the same nitrogen atom are taken together to form an optionally substituted ring selected from 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 0-2 additional heteroatoms independently selected from nitrogen, oxygen, and sulfur, and 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 0-3 additional heteroatoms independently selected from nitrogen, oxygen, and sulfur.

In some embodiments, each R ^A1 is independently C1-6 aliphatic or 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein each C1-6 aliphatic and 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl of R ^A1 is optionally substituted with 1, 2, 3, 4, 5, or 6 independently selected R ^G1 substituents.

In some embodiments, each R ^A1 is independently C1-6 aliphatic or 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein each C1-6 aliphatic and 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl of R ^A1 is optionally substituted with 1 or 2 independently selected R ^G1 substituents.

In some embodiments, each R ^A1 is independently C1-6 alkyl or 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1-3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein each C1-6 aliphatic and 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl of R ^A1 is optionally substituted with 1, 2, 3, 4, 5, or 6 independently selected R ^G1 substituents.

In some embodiments, each R ^A1 is independently C1-6 alkyl or 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein each C1-6 aliphatic and 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl of R ^A1 is optionally substituted with 1 or 2 independently selected R ^G1 substituents.

In some embodiments, each R ^A1 is independently C1-6 aliphatic, wherein each Ci-6 aliphatic of R ^A1 is optionally substituted with 1, 2, 3, 4, 5, or 6 independently selected R ^G1 substituents.

In some embodiments, each R ^A1 is independently C1-6 alkyl, wherein each C1-6 alkyl of R ^A1 is optionally substituted with 1, 2, 3, 4, 5, or 6 independently selected R ^G1 substituents. In some embodiments, each R ^A1 is independently C1-6 alkyl, wherein each C1-6 alkyl of R ^A1 is optionally substituted with 1, 2, 3, or 4 independently selected R ^G1 substituents. In some embodiments, each R ^A1 is independently C1-6 alkyl, wherein each C1-6 alkyl of R ^A1 is optionally substituted with 1 or 2 independently selected R ^G1 substituents.

In some embodiments, each R ^G1 is independently selected from OR, where each R is hydrogen or C1-6 aliphatic. In some embodiments, each R ^G1 is independently selected from OR, where each R is hydrogen or C1-6 alkyl. In some embodiments, each R ^G1 is independently selected from OR, where each R is C1-6 alkyl. In some embodiments, each R ^G1 is independently selected from OR, where each R is C1-3 alkyl. In some embodiments, each R ^G1 is hydroxy or methoxy. In some embodiments, each R ^G1 is methoxy. In some embodiments, each R ^G1 is hydroxy.

In some embodiments, each R ^A1 is independently C1-6 aliphatic or 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein each C1-6 alkyl and 3- to 7- membered saturated or partially unsaturated monocyclic heterocyclyl of R ^A1 is optionally substituted with hydroxy or methoxy.

In some embodiments, each R ^A1 is independently C1-6 alkyl or 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1-3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein each C1-6 alkyl and 3- to 7- membered saturated or partially unsaturated monocyclic heterocyclyl of R ^A1 is optionally substituted with hydroxy or methoxy.

In some embodiments, each R ^A1 is independently C1-6 alkyl, wherein each C1-6 alkyl of R ^A1 is optionally substituted with methoxy. In some embodiments, each R ^AG is independently halogen, -CN, -OR, -SR, -N(R)2, - C(O)R, -C(O)OR, -C(O)N(R) ₂ , -C(O)NR(OR), -OC(O)R, -OC(O)N(R) ₂ , -OC(O)OR, - OSO ₂ R, -OSO ₂ N(R) ₂ , -N(R)C(O)R, -NRC(O)OR, -NRC(O)N(R) ₂ , -N(R)SO ₂ R, - NRS(O) ₂ N(R) ₂ , -NROR, -NRS(O)R, -NRS(O)N(R) ₂ , -S(O)R, -SO ₂ R, -S(O)N(R) ₂ , -SO ₂ N(R) ₂ , -SO ₃ R, -C(=NR ^m )R, -C(=NR ^m )N(R) ₂ , -NRC(=NR ^m )R, -NRC(=NR ^m )N(R) ₂ , - NRS(O)(=NR ^m )R, -NRS(O)(=NR ^m )N(R) ₂ , -OS(O)(=NR ^m )R, -S(O)(=NR ^m )R, - S(O)(=NR ^m )N(R) ₂ , -P(O)(R) ₂ , or an optionally substituted group selected from C1-6 aliphatic, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 10- membered saturated or partially unsaturated bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1-3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 10- membered saturated or partially unsaturated bicyclic heterocyclyl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 6-membered monocyclic heteroaryl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, and 8- to 10-membered bicyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur.

In some embodiments, each R ^AG is independently selected from halogen and C1-6 aliphatic. In some embodiments, each R ^AG is an independently selected halogen. In some embodiments, each R ^AG is an independently selected C1-6 aliphatic. In some embodiments, each R ^AG is an independently selected C1-6 alkyl. In some embodiments, each R ^AG is an independently selected C1-3 alkyl. In some embodiments, each R ^AG is independently selected from fluoro and methyl. In some embodiments, each R ^AG is fluoro. In some embodiments, each R ^AG is methyl.

In some embodiments, each R ^A is independently selected from methyl, ethyl, n- propyl, isopropyl, etheneyl, methoxy, methoxyethoxy, dimethylamino, dimethylaminocarbonyl, azetidinylcarbonyl, (hydroxyazetidinyl)carbonyl, cyclopropyl, cyclopentyl, phenyl, pyridyl, pyrimidinyl, tetrahydrofuranyl, tetrahydropyranyl, azetidinyl, pyrrolidinyl, piperidinyl, tetrahydro-2H-pyranyl, morpholinyl, tetrahydrofuranyl, piperazinyl, 5,6-dihydro-[l,2,4]triazolo[l,5-a]pyrazin-7(8H)-yl, wherein the methyl, ethyl, n-propyl, isopropyl, etheneyl, cyclopropyl, cyclopentyl, phenyl, pyridyl, pyrimidinyl, tetrahydrofuranyl, tetrahydropyranyl, azetidinyl, pyrrolidinyl, piperidinyl, tetrahydro-2H-pyranyl, morpholinyl, tetrahydrofuranyl, piperazinyl, and 5,6-dihydro-[l,2,4]triazolo[l,5-a]pyrazin-7(8H)-yl are each optionally substituted with 1, 2, 3, or 4 independently selected R ^AG substituents.

In some embodiments, each R ^A is independently selected from methyl, ethyl, n- propyl, isopropyl, etheneyl, methoxy, methoxyethoxy, dimethylamino, dimethylaminocarbonyl, azetidinylcarbonyl, (hydroxyazetidinyl)carbonyl, cyclopropyl, cyclopentyl, phenyl, pyridyl, pyrimidinyl, tetrahydrofuranyl, tetrahydropyranyl, azetidinyl, pyrrolidinyl, piperidinyl, tetrahydro-2H-pyranyl, morpholinyl, tetrahydrofuranyl, piperazinyl, 5,6-dihydro-[l,2,4]triazolo[l,5-a]pyrazin-7(8H)-yl, wherein the methyl, ethyl, n-propyl, isopropyl, etheneyl, cyclopropyl, cyclopentyl, phenyl, pyridyl, pyrimidinyl, tetrahydrofuranyl, tetrahydropyranyl, azetidinyl, pyrrolidinyl, piperidinyl, tetrahydro-2H-pyranyl, morpholinyl, tetrahydrofuranyl, piperazinyl, and 5,6-dihydro-[l,2,4]triazolo[l,5-a]pyrazin-7(8H)-yl are each optionally substituted with 1 or 2 independently selected R ^AG substituents.

In some embodiments, each R ^A is independently selected from methyl, ethyl, n- propyl, isopropyl, etheneyl, methoxy, methoxyethoxy, dimethylamino, cyclopropyl, cyclopentyl, phenyl, methylphenyl, pyridyl, fluoropyridyl, methylpyridyl, pyrimidinyl, tetrahydrofuranyl, and tetrahydropyranyl.

In some embodiments, each R ^A is independently selected from methyl, ethyl, propyl, etheneyl, methoxy, methoxyethoxy, dimethylamino, phenyl, methylphenyl, pyridyl, fluoropyridyl, methylpyridyl, pyrimidinyl, and tetrahydropyranyl.

In some embodiments, each L ^A is a covalent bond or a C1-3 aliphatic. In some embodiments, each L ^A is a covalent bond or a C1-2 aliphatic. In some embodiments, each L ^A is a covalent bond. In some embodiments, each L ^A is a C1-3 aliphatic. In some embodiments, each L ^A is a C1-2 aliphatic.

In some embodiments, each L ^A is a covalent bond or a C1-2 aliphatic, and each R ^A is independently selected from methyl, ethyl, n-propyl, isopropyl, etheneyl, methoxy, methoxyethoxy, dimethylamino, dimethylaminocarbonyl, azetidinylcarbonyl, (hydroxyazetidinyl)carbonyl, cyclopropyl, cyclopentyl, phenyl, pyridyl, pyrimidinyl, tetrahydrofuranyl, tetrahydropyranyl, azetidinyl, pyrrolidinyl, piperidinyl, tetrahydro -2H- pyranyl, morpholinyl, tetrahydrofuranyl, piperazinyl, 5,6-dihydro-[l,2,4]triazolo[l,5- a]pyrazin-7(8H)-yl, wherein the methyl, ethyl, n-propyl, isopropyl, etheneyl, cyclopropyl, cyclopentyl, phenyl, pyridyl, pyrimidinyl, tetrahydrofuranyl, tetrahydropyranyl, azetidinyl, pyrrolidinyl, piperidinyl, tetrahydro-2H-pyranyl, morpholinyl, tetrahydrofuranyl, piperazinyl, and 5,6-dihydro-[l,2,4]triazolo[l,5-a]pyrazin-7(8H)-yl are each optionally substituted with 1 or 2 independently selected R ^AG substituents.

In some embodiments, each L ^A is a covalent bond or a C2 aliphatic, and each R ^A is independently selected from methyl, ethyl, propyl, etheneyl, methoxy, methoxyethoxy, dimethylamino, phenyl, methylphenyl, pyridyl, fluoropyridyl, methylpyridyl, pyrimidinyl, and tetrahydropyranyl. In some embodiments, each L ^A is a covalent bond and each R ^A is independently selected from methyl, ethyl, propyl, etheneyl, methoxy, methoxyethoxy, dimethylamino, phenyl, methylphenyl, pyridyl, fluoropyridyl, methylpyridyl, pyrimidinyl, and tetrahydropyranyl .

In some embodiments, each L ^A is a C2 aliphatic and each R ^A is independently selected from methyl, ethyl, propyl, etheneyl, methoxy, methoxyethoxy, dimethylamino, phenyl, methylphenyl, pyridyl, fluoropyridyl, methylpyridyl, pyrimidinyl, and tetrahydropyranyl. In some embodiments, each L ^A is a C2 aliphatic and each R ^A is phenyl.

In some embodiments of any of Formulae I, II, III, IV, VI, and Via, R ¹ is hydrogen or an optionally substituted group selected from C1-6 aliphatic, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, phenyl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, and 5- to 6-membered monocyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur.

As described above, in some embodiments of any of Formulae I, II, and III, R ¹ is hydrogen or an optionally substituted group selected from C1-6 aliphatic, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, phenyl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, and 5- to 6-membered monocyclic heteroaryl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur.

In some embodiments, R ¹ is an optionally substituted C1-6 aliphatic, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, or 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1-3 heteroatoms independently selected from nitrogen, oxygen, and sulfur.

In some embodiments, R ¹ is an optionally substituted C1-6 alkyl, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, or 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1-3 heteroatoms independently selected from nitrogen, oxygen, and sulfur.

In some embodiments, R ¹ is a 3 - to 7-membered saturated or partially unsaturated monocyclic carbocyclyl. In some embodiments, R ¹ is cyclopropyl. In some embodiments, R ¹ is a 3 - to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur. In some embodiments, R ¹ is tetrahydrofuranyl.

In some embodiments, R ¹ is optionally substituted C1-6 aliphatic. In some embodiments, R ¹ is optionally substituted C1-3 aliphatic. In some embodiments, R ¹ is optionally substituted C 1-2 aliphatic.

In some embodiments, R ¹ is C1-6 alkyl. In some embodiments, R ¹ is C1-3 alkyl. In some embodiments, R ¹ is C1-2 alkyl. In some embodiments, R ¹ is optionally substituted C1-2 alkyl. In some embodiments, R ¹ is C1-2 alkyl, which is optionally substituted with 1, 2, 3, or 4 halogen groups. In some embodiments, R ¹ is optionally substituted C1-2 alkyl. In some embodiments, R ¹ is C1-2 alkyl, which is optionally substituted with 1 or 2 halogen groups. In some embodiments, R ¹ is optionally substituted Ci aliphatic. In some embodiments, R ¹ is methyl, ethyl, or difluoroethyl. In some embodiments, R ¹ is methyl or ethyl. In some embodiments, R ¹ is methyl. In some embodiments, R ¹ is ethyl. In some embodiments, R ¹ is difluoroethyl.

In some embodiments, R ¹ is methyl, trideuteromethyl, ethyl, difluoroethyl, hydroxyethyl, cyclopropyl, or tetrahydrofuranyl.

In some embodiments, R ¹ and one of L ^A -R ^A taken together with the atoms to which they are attached form a 3 - to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, or 5- to 14-membered saturated or partially unsaturated bicyclic heterocyclyl having 1- 4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein each of the 3- to 7-membered monocyclic heterocyclyl and 5- to 14-membered bicyclic heterocyclyl is independently optionally substituted with 1, 2, 3, 4, 5, or 6 R ^AG substituents.

In some embodiments, R ¹ and one of L ^A -R ^A taken together with the atoms to which they are attached form a 3 - to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, which is optionally substituted with 1, 2, 3, 4, 5, or 6 R ^AG substituents.

In some embodiments, R ¹ and one of L ^A -R ^A taken together with the atoms to which they are attached form a 3 - to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, which is optionally substituted with 1, 2, 3, or 4 R ^AG substituents.

In some embodiments, R ¹ and one of L ^A -R ^A taken together with the atoms to which they are attached form a 3 - to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, which is optionally substituted with 1 or 2 R ^AG substituents.

In some embodiments, R ¹ and one of L ^A -R ^A taken together with the atoms to which they are attached form a 6-membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, which is optionally substituted with 1, 2, 3, 4, 5, or 6 R ^AG substituents.

In some embodiments, R ¹ and one of L ^A -R ^A taken together with the atoms to which they are attached form a 6-membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, which is optionally substituted with 1, 2, 3, or 4 R ^AG substituents.

In some embodiments, R ¹ and one of L ^A -R ^A taken together with the atoms to which they are attached form a 6-membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, which is optionally substituted with 1 or 2 R ^AG substituents.

In some embodiments, R ¹ and one of L ^A -R ^A taken together with the atoms to which they are attached form a 6-membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, which is optionally substituted with 1 or 2 R ^AG substituents.

In some embodiments, the compound of Formula l is a compound of Formula IV : or a pharmaceutically acceptable salt thereof.

In some embodiments of Formula IV :

L ⁵ is a bivalent C1-3 aliphatic which is optionally substituted with 1, 2, 3, or 4 independently selected R ^L5A substituents; or L ⁵ and one of L ^A -R ^A taken together with the atoms to which they are attached form a 3 - to 16-membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 16- membered saturated or partially unsaturated bicyclic carbocyclyl, 3- to 16-membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, or 5- to 16-membered saturated or partially unsaturated bicyclic heterocyclyl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein each of the 3- to 16-membered monocyclic carbocyclyl, 5- to 16-membered bicyclic carbocyclyl, 3- to 16 -membered monocyclic heterocyclyl, and 5- to 16-membered bicyclic heterocyclyl is independently optionally substituted with 1, 2, 3, 4, 5, or 6 R ^AG substituents;

Ring B is 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 6- membered monocyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, or 8- to 10-membered bicyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur; and m is 0, 1, 2, 3, 4, 5, or 6.

In some embodiments of Formula IV :

L ⁵ is a bivalent C1-3 aliphatic which is optionally substituted with 1, 2, 3, or 4 independently selected R ^L5A substituents; or L ⁵ and one of L ^A -R ^A taken together with the atoms to which they are attached form a 3 - to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 10- membered saturated or partially unsaturated bicyclic carbocyclyl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, or 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein each of the 3- to 7-membered monocyclic carbocyclyl, 5- to 10-membered bicyclic carbocyclyl, 3- to 7-membered monocyclic heterocyclyl, and 5- to 10-membered bicyclic heterocyclyl is independently optionally substituted with 1, 2, 3, 4, 5, or 6 R ^AG substituents;

Ring B is 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 6- membered monocyclic heteroaryl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, or 8- to 10-membered bicyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur; and m is 0, 1, 2, 3, 4, 5, or 6.

In some embodiments of any of Formulae I, II, III, IV, V, Va, VI, Via, VII, Vila, and Vllb, Z is N or CR ² . In some embodiments of any of Formulae I, II, III, IV, V, Va, VI, and Via, Z is N or CR ² . As described above, in some embodiments of any of Formulae I, II, and III, Z is N or CR ² . In some embodiments, Z is CR ² . In some embodiments, Z is CH.

In some embodiments, R ² is hydrogen, halogen, -CN, -OR, -SR, -N(R)2, -C(O)R, - C(O)N(R) ₂ , -C(O)NR(OR), -OC(O)R, -OC(O)N(R) ₂ , -OC(O)OR, -OSO ₂ R, -OSO ₂ N(R) ₂ , - N(R)C(O)R, -NRC(O)OR, -NRC(O)N(R) ₂ , -N(R)SO ₂ R, -NRS(O) ₂ N(R) ₂ , -NROR, - NRS(O)R, -NRS(O)N(R) ₂ , -S(O)R, -SO ₂ R, -S(O)N(R) ₂ , -SO ₂ N(R) ₂ , -SO ₃ R, -C(=NR ^m )R, - C(=NR ^m )N(R) ₂ , -NRC(=NR ^m )R, -NRC(=NR ^m )N(R) ₂ , -NRS(O)(=NR ^m )R, - NRS(O)(=NR ^m )N(R) ₂ , -OS(O)(=NR ^m )R, -S(O)(=NR ^m )R, -S(O)(=NR ^m )N(R) ₂ , -P(O)(R) ₂ , or an optionally substituted group selected from C1-6 aliphatic, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, phenyl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, and 5- to 6-membered monocyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur.

In some embodiments, R ² is hydrogen.

In some embodiments of any of Formulae I, II, III, IV, V, Va, VI, Via, VII, Vila, and Vllb, R ³ is -F, -Cl, -CN, -OR, -SR, -N(R) ₂ , -C(O)R, -C(O)N(R) ₂ , -C(O)NR(OR), -OC(O)R, - OC(O)N(R) ₂ , -OC(O)OR, -OSO ₂ R, -OSO ₂ N(R) ₂ , -N(R)C(O)R, -NRC(O)OR, -NRC(O)N(R) _2; -N(R)SO ₂ R, -NRS(O) ₂ N(R) ₂ , -NROR, -NRS(O)R, -NRS(O)N(R) ₂ , -S(O)R, -SO ₂ R, - S(O)N(R) ₂ , -SO ₂ N(R) ₂ , -SO ₃ R, -C(=NR ^m )R, -C(=NR ^m )N(R) ₂ , -NRC(=NR ^m )R, - NRC(=NR ^m )N(R) ₂ , -NRS(O)(=NR ^m )R, -NRS(O)(=NR ^m )N(R) ₂ , -OS(O)(=NR ^m )R, - S(O)(=NR ^m )R, -S(O)(=NR ^m )N(R) ₂ , -P(O)(R) ₂ , or an optionally substituted group selected from C1-6 aliphatic, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, phenyl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, and 5- to 6-membered monocyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur.

In some embodiments of any of Formulae I, II, III, IV, V, Va, VI, and Via, R ³ is -F, - Cl, -CN, -OR, -SR, -N(R) ₂ , -C(O)R, -C(O)N(R) ₂ , -C(O)NR(OR), -OC(O)R, -OC(O)N(R) ₂ , - OC(O)OR, -OSO2R, -OSO ₂ N(R) ₂ , -N(R)C(O)R, -NRC(O)OR, -NRC(O)N(R) ₂ , -N(R)SO ₂ R, - NRS(O) ₂ N(R) ₂ , -NROR, -NRS(O)R, -NRS(O)N(R) ₂ , -S(O)R, -SO ₂ R, -S(O)N(R) ₂ , -SO ₂ N(R) ₂ , -SO3R, -C(=NR ^m )R, -C(=NR ^m )N(R) ₂ , -NRC(=NR ^m )R, -NRC(=NR ^m )N(R) ₂ , - NRS(O)(=NR ^m )R, -NRS(O)(=NR ^m )N(R) ₂ , -OS(O)(=NR ^m )R, -S(O)(=NR ^m )R, - S(O)(=NR ^m )N(R) ₂ , -P(O)(R) ₂ , or an optionally substituted group selected from C1-6 aliphatic, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, phenyl, 3- to 7- membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, and 5- to 6-membered monocyclic heteroaryl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur.

As described above, in some embodiments of any of Formulae I, II, and III, R ³ is -F, - Cl, -CN, -OR, -SR, -N(R) ₂ , -C(O)R, -C(O)N(R) ₂ , -C(O)NR(OR), -OC(O)R, -OC(O)N(R) ₂ , - OC(O)OR, -OSO ₂ R, -OSO ₂ N(R) ₂ , -N(R)C(O)R, -NRC(O)OR, -NRC(O)N(R) ₂ , -N(R)SO ₂ R, - NRS(O) ₂ N(R) ₂ , -NROR, -NRS(O)R, -NRS(O)N(R) ₂ , -S(O)R, -SO ₂ R, -S(O)N(R) ₂ , -SO ₂ N(R) ₂ , -SO3R, -C(=NR ^m )R, -C(=NR ^m )N(R) ₂ , -NRC(=NR ^m )R, -NRC(=NR ^m )N(R) ₂ , - NRS(O)(=NR ^m )R, -NRS(O)(=NR ^m )N(R) ₂ , -OS(O)(=NR ^m )R, -S(O)(=NR ^m )R, - S(O)(=NR ^m )N(R) ₂ , -P(O)(R) ₂ , or an optionally substituted group selected from C1-6 aliphatic, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, phenyl, 3- to 7- membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, and 5- to 6-membered monocyclic heteroaryl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur.

In some embodiments, R ³ is -F, -Cl, -CN, or optionally substituted C 1-6 aliphatic. In some embodiments, R ³ is -F, -Cl, or -CN. In some embodiments, R ³ is -F or -Cl. In some embodiments, R ³ is optionally substituted C1-6 aliphatic. In some embodiments, R ³ is optionally substituted C 1-6 aliphatic or -Cl. In some embodiments, R ³ is optionally substituted C1-3 aliphatic. In some embodiments, R ³ is optionally substituted C1-3 aliphatic or -Cl. In some embodiments, R ³ is optionally substituted C1-2 aliphatic. In some embodiments, R ³ is optionally substituted C1-2 aliphatic or -Cl. In some embodiments, R ³ is optionally substituted Ci aliphatic. In some embodiments, R ³ is methyl, -CF3, or -CF ₂ H. In some embodiments, R ³ is methyl. In some embodiments, R ³ is -Cl. In some embodiments, R ³ is methyl or -Cl. In some embodiments, R ³ is methyl or -CF3. In some embodiments, R ³ is -CF3 (i.e., trifluoromethyl).

In some embodiments of any of Formulae I, II, III, IV, V, Va, VI, Via, VII, Vllb, and Vllb, U is N or CR ⁴ . In some embodiments of any of Formulae I, II, III, IV, V, Va, VI, and Via, U is N or CR ⁴ . As described above, in some embodiments of any of Formulae I, II, and III, U is N or CR ⁴ . In some embodiments, U is CR ⁴ . In some embodiments, U is CH. In some embodiments, R ⁴ is hydrogen, halogen, -CN, -OR, -SR, -N(R)2, -C(O)R, - C(O)N(R) ₂ , -C(O)NR(OR), -OC(O)R, -OC(O)N(R) ₂ , -OC(O)OR, -OSO ₂ R, -OSO ₂ N(R) ₂ , - N(R)C(O)R, -NRC(O)OR, -NRC(O)N(R) ₂ , -N(R)SO ₂ R, -NRS(O) ₂ N(R) ₂ , -NROR, - NRS(O)R, -NRS(O)N(R) ₂ , -S(O)R, -SO ₂ R, -S(O)N(R) ₂ , -SO ₂ N(R) ₂ , -SO ₃ R, -C(=NR ^m )R, - C(=NR ^m )N(R) ₂ , -NRC(=NR ^m )R, -NRC(=NR ^m )N(R) ₂ , -NRS(O)(=NR ^m )R, - NRS(O)(=NR ^m )N(R) ₂ , -OS(O)(=NR ^m )R, -S(O)(=NR ^m )R, -S(O)(=NR ^m )N(R) ₂ , -P(O)(R) ₂ , or an optionally substituted group selected from C1-6 aliphatic, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, phenyl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, and 5- to 6-membered monocyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur.

In some embodiments, R ⁴ is hydrogen.

As described above, in some embodiments of Formula I, R ⁵ is -L ⁵ -R ^5A .

As described above, in some embodiments of any of Formula I, II, and III, L ⁵ is a covalent bond or optionally substituted bivalent C1-6 aliphatic. In some embodiments, L ⁵ is optionally substituted bivalent C1-6 aliphatic. In some embodiments, L ⁵ is a bivalent Ci-6 aliphatic, which is optionally substituted by hydroxy. In some embodiments, L ⁵ is optionally substituted bivalent C1-3 aliphatic. In some embodiments, L ⁵ is a bivalent C1-3 aliphatic, which is optionally substituted by hydroxy. In some embodiments, L ⁵ is optionally substituted bivalent C1-2 aliphatic. In some embodiments, L ⁵ is bivalent C1-2 aliphatic, which is optionally substituted by hydroxy.

In some embodiments, L ⁵ is bivalent C1-2 aliphatic, which is optionally substituted by hydroxy or 5-6 membered saturated or partially unsaturated monocyclic heterocyclyl having 1-3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, and wherein the 5- 6 membered saturated or partially unsaturated monocyclic heterocyclyl is optionally substituted by C1-6 alkyl.

In some embodiments, L ⁵ is bivalent C1-2 aliphatic, which is optionally substituted by hydroxy or 5-6 membered saturated or partially unsaturated monocyclic heterocyclyl having 1-3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, and wherein the 5- 6 membered saturated or partially unsaturated monocyclic heterocyclyl is optionally substituted by C1-3 alkyl.

In some embodiments, L ⁵ is bivalent C1-2 aliphatic, which is optionally substituted by hydroxy or piperazinyl, wherein the piperazinyl is optionally substituted by C1-6 alkyl.

In some embodiments, L ⁵ is bivalent C1-2 aliphatic, which is optionally substituted by hydroxy or piperazinyl, wherein the piperazinyl is optionally substituted by C1-3 alkyl. In some embodiments, L ⁵ is bivalent C1-2 aliphatic, which is optionally substituted by hydroxy or methylpiperazinyl.

In some embodiments, L ⁵ is -CH(CH3)-. In some embodiments, L ⁵ is -CH(CH3)- or - CH(CH20H)-. In some embodiments, L ⁵ is -CH(CH20H)-. In some embodiments, L ⁵ is -CH2-

In some embodiments, L ⁵ and one of L ^A -R ^A taken together with the atoms to which they are attached form a 3 - to 16-membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 16-membered saturated or partially unsaturated bicyclic carbocyclyl, 3- to 16-membered saturated or partially unsaturated monocyclic heterocyclyl having 1-3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, or 5- to 16-membered saturated or partially unsaturated bicyclic heterocyclyl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein each of the 3- to 16-membered monocyclic carbocyclyl, 5 - to 16-membered bicyclic carbocyclyl, 3 - to 16-membered monocyclic heterocyclyl, and 5- to 16-membered bicyclic heterocyclyl is independently optionally substituted with 1, 2, 3, 4, 5, or 6 R ^AG substituents.

In some embodiments, L ⁵ and one of L ^A -R ^A taken together with the atoms to which they are attached form a 3 - to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic carbocyclyl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1-3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, or 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein each of the 3- to 7-membered monocyclic carbocyclyl, 5- to 10-membered bicyclic carbocyclyl, 3- to 7-membered monocyclic heterocyclyl, and 5- to 10-membered bicyclic heterocyclyl is independently optionally substituted with 1, 2, 3, 4, 5, or 6 R ^AG substituents.

As described above, in some embodiments of Formula I, R ^5A is -OR ^5A11 , -SR ^5A1 , - NHR ^5A12 , -N(R ^5A12 ) ₂ , -C(O)R ^5A1 , -C(O)N(R ^5A1 ) ₂ , -C(O)NR ^5A1 (OR ^5A1 ), -OC(O)R ^5A1 , - OC(O)N(R ^5A1 ) ₂ , -OC(O)OR ^5A1 , -OSO ₂ R ^5A1 , -OSO ₂ N(R ^5A1 ) ₂ , -N(R ^5A1 )C(O)R ^5A1 , - NR ^5A1 C(O)OR ^5A1 , -NR ^5A1 C(O)N(R ^5A1 ) ₂ , -N(R ^5A1 )SO ₂ R ^5A1 , -NR ^5A1 S(O) ₂ N(R ^5A1 ) ₂ , - NR ^5A1 OR ^5A1 , -NR ^5A1 S(O)R ^5A1 , -NR ^5A1 S(O)N(R ^5A1 ) ₂ , -S(O)R ^5A1 , -SO ₂ R ^5A1 , -S(O)N(R ^5A1 ) ₂ , - SO ₂ N(R ^5A1 ) ₂ , -SO ₃ R ^5A1 , -C(=NR ^m )R ^5A1 , -C(=NR ^m )N(R ^5A1 ) ₂ , -NR ^5A1 C(=NR ^m )R ^5A1 , - NR ^5A1 C(=NR ^m )N(R ^5A1 ) ₂ , -NR ^5A1 S(O)(=NR ^m )R ^5A1 , -NR ^5A1 S(O)(=NR ^m )N(R ^5A1 ) ₂ , - OS(O)(=NR ^m )R ^5A1 , -S(O)(=NR ^m )R ^5A1 , -S(O)(=NR ^m )N(R ^5A1 ) ₂ , -P(O)(R ^5A1 ) ₂ , methyl, C _2.6 aliphatic, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic carbocyclyl, phenyl, 8- to 10- membered bicyclic aryl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 6-membered monocyclic heteroaryl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, or 8- to 10-membered bicyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein each of the C ₂ -6 aliphatic, 3- to 7- membered monocyclic carbocyclyl, 5- to 10-membered bicyclic carbocyclyl, phenyl, 8- to 10- membered bicyclic aryl, 3- to 7-membered monocyclic heterocyclyl, 5- to 10-membered bicyclic heterocyclyl, 5- to 6-membered monocyclic heteroaryl, and 8- to 10-membered bicyclic heteroaryl of R ^5A is independently optionally substituted with 1, 2, 3, 4, 5, or 6 R ^5AG substituents; or the methyl of R ^5A is substituted with 1, 2, or 3 R ^5AG substituents.

In some embodiments, R ^5A is -NHR ^5A12 or -OR ^5A11 .

In some embodiments, R ^5A is -OR ^5A11 .

In some embodiments, R ^5A is -NHR ^5A12 .

In some embodiments of any of Formulae I, II, III, IV, V, Va, VI, Via, VII, and Vila each R ^5A12 is independently C1-6 aliphatic, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 6-membered monocyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, or 8- to 10-membered bicyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein each of the C1-6 aliphatic, 3- to 7-membered monocyclic carbocyclyl, 5- to 10-membered bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7-membered monocyclic heterocyclyl, 5- to 10-membered bicyclic heterocyclyl, 5- to 6-membered monocyclic heteroaryl, and 8- to 10-membered bicyclic heteroaryl of R ^5A12 is independently optionally substituted with 1, 2, 3, 4, 5, or 6 R ^G1 substituents; or two R ^5A12 when attached to the same nitrogen atom are taken together to form an optionally substituted ring selected from 3- to 7- membered saturated or partially unsaturated monocyclic heterocyclyl having 0-2 additional heteroatoms independently selected from nitrogen, oxygen, and sulfur, and 5- to 10- membered saturated or partially unsaturated bicyclic heterocyclyl having 0-3 additional heteroatoms independently selected from nitrogen, oxygen, and sulfur.

In some embodiments of any of Formulae I, II, III, IV, V, Va, VI, and Via, each R ^5A12 is independently C1-6 aliphatic, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 6-membered monocyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, or 8- to 10-membered bicyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein each of the C1-6 aliphatic, 3- to 7-membered monocyclic carbocyclyl, 5- to 10-membered bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7-membered monocyclic heterocyclyl, 5- to 10-membered bicyclic heterocyclyl, 5- to 6-membered monocyclic heteroaryl, and 8- to 10-membered bicyclic heteroaryl of R ^5A12 is independently optionally substituted with 1, 2, 3, 4, 5, or 6 R ^G1 substituents; or two R ^5A12 when attached to the same nitrogen atom are taken together to form an optionally substituted ring selected from 3- to 7- membered saturated or partially unsaturated monocyclic heterocyclyl having 0-2 additional heteroatoms independently selected from nitrogen, oxygen, and sulfur, and 5- to 10- membered saturated or partially unsaturated bicyclic heterocyclyl having 0-3 additional heteroatoms independently selected from nitrogen, oxygen, and sulfur.

In some embodiments of any of Formulae I, II, and III, each R ^5A12 is independently C1-6 aliphatic, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic carbocyclyl, phenyl, 8- to 10- membered bicyclic aryl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 6-membered monocyclic heteroaryl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, or 8- to 10-membered bicyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein each of the C1-6 aliphatic, 3- to 7- membered monocyclic carbocyclyl, 5- to 10-membered bicyclic carbocyclyl, phenyl, 8- to 10- membered bicyclic aryl, 3- to 7-membered monocyclic heterocyclyl, 5- to 10-membered bicyclic heterocyclyl, 5- to 6-membered monocyclic heteroaryl, and 8- to 10-membered bicyclic heteroaryl of R ^5A12 is independently optionally substituted with 1, 2, 3, 4, 5, or 6 R ^G1 substituents; or two R ^5A12 when attached to the same nitrogen atom are taken together to form an optionally substituted ring selected from 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 0-2 additional heteroatoms independently selected from nitrogen, oxygen, and sulfur, and 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 0-3 additional heteroatoms independently selected from nitrogen, oxygen, and sulfur.

In some embodiments, each R ^5A12 is independently 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 6-membered monocyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, or 8- to 10-membered bicyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein each of the 3- to 7-membered monocyclic carbocyclyl, 5- to 10-membered bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7-membered monocyclic heterocyclyl, 5- to 10-membered bicyclic heterocyclyl, 5- to 6-membered monocyclic heteroaryl, and 8- to 10-membered bicyclic heteroaryl of R ^5A12 is independently optionally substituted with 1, 2, 3, 4, 5, or 6 R ^G1 substituents.

In some embodiments, each R ^5A12 is independently phenyl, 8- to 10-membered bicyclic aryl, 5- to 6-membered monocyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, or 8- to 10-membered bicyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein each of the phenyl, 8- to 10-membered bicyclic aryl, 5- to 6-membered monocyclic heteroaryl, and 8- to 10-membered bicyclic heteroaryl of R ^5A12 is independently optionally substituted with 1, 2, 3, 4, 5, or 6 R ^G1 substituents.

In some embodiments, each R ^5A12 is independently phenyl or 5- to 6-membered monocyclic heteroaryl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein each of the phenyl and 5- to 6-membered monocyclic heteroaryl of R ^5A12 is independently optionally substituted with 1, 2, 3, 4, 5, or 6 R ^G1 substituents.

In some embodiments, each R ^5A12 is independently phenyl or pyridinyl, each of which is optionally substituted with 1, 2, 3, 4, 5, or 6 independently selected R ^G1 substituents. In some embodiments, each R ^5A12 is independently phenyl optionally substituted with 1, 2, 3, 4, or 5 independntly selected R ^G1 substituents.

In some embodiments, a single instance of R ^5A12 is phenyl or pyridinyl, each of which is optionally substituted with 1, 2, or 3 independently selected R ^G1 substituents. In some embodiments, a single instance of R ^5A12 is phenyl or pyridinyl, each of which is optionally substituted with 1 or 2 independently selected R ^G1 substituents. In some embodiments, a single instance of R ^5A12 is phenyl or pyridinyl, each of which is optionally substituted with 1 R ^G1 substituent.

In some embodiments, each R ^5A12 is independently phenyl optionally substituted with 1, 2, 3, 4, 5, or 6 independently selected R ^G1 substituents. In some embodiments, a single instance of R ^5A12 is phenyl substituted with 1, 2, or 3 independently selected R ^G1 substituents. In some embodiments, a single instance of R ^5A12 is phenyl substituted with 1 or 2 independently selected R ^G1 substituents. In some embodiments, a single instance of R ^5A12 is phenyl substituted with 1 R ^G1 substituent.

In some embodiments, each R ^5A12 is independently pyridinyl optionally substituted with 1, 2, 3, 4, 5, or 6 independently selected R ^G1 substituents. In some embodiments, a single instance of R ^5A12 is pyridinyl substituted with 1, 2, or 3 independently selected R ^G1 substituents. In some embodiments, a single instance of R ^5A12 is pyridinyl, which is optionally substituted with 1 or 2 independently selected R ^G1 substituents. In some embodiments, a single instance of R ^5A12 is pyridinyl substituted with 1 or 2 independently selected R ^G1 substituents. In some embodiments, a single instance of R ^5A12 is pyridinyl substituted with 1 R ^G1 substituent.

In some embodiments of Formula I, each R ^5A1 is independently hydrogen, Ci-6 aliphatic, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic carbocyclyl, phenyl, 8- to 10- membered bicyclic aryl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 6-membered monocyclic heteroaryl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, or 8- to 10-membered bicyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein each of the C1-6 aliphatic, 3- to 7- membered monocyclic carbocyclyl, 5- to 10-membered bicyclic carbocyclyl, phenyl, 8- to 10- membered bicyclic aryl, 3- to 7-membered monocyclic heterocyclyl, 5- to 10-membered bicyclic heterocyclyl, 5- to 6-membered monocyclic heteroaryl, and 8- to 10-membered bicyclic heteroaryl of R ^5A1 is independently optionally substituted with 1, 2, 3, 4, 5, or 6 R ^G1 substituents; or two R ^5A1 when attached to the same nitrogen atom are taken together to form an optionally substituted ring selected from 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 0-2 additional heteroatoms independently selected from nitrogen, oxygen, and sulfur, and 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 0-3 additional heteroatoms independently selected from nitrogen, oxygen, and sulfur.

As described above, in some embodiments of Formula I, R ^5A11 is methyl, C2-6 aliphatic, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic carbocyclyl, phenyl, 8- to 10- membered bicyclic aryl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 6-membered monocyclic heteroaryl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, or 8- to 10-membered bicyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein each of the C2-6 aliphatic, 3- to 7- membered monocyclic carbocyclyl, 5- to 10-membered bicyclic carbocyclyl, phenyl, 8- to 10- membered bicyclic aryl, 3- to 7-membered monocyclic heterocyclyl, 5- to 10-membered bicyclic heterocyclyl, 5- to 6-membered monocyclic heteroaryl, and 8- to 10-membered bicyclic heteroaryl of R ^5A11 is independently optionally substituted with 1, 2, 3, 4, 5, or 6 R ^G1 substituents; or the methyl of R ^5A11 is substituted with 1, 2, or 3 R ^G1 substituents.

In some embodiments, R ^5A11 is 5- to 6-membered monocyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, which is independently optionally substituted with 1, 2, 3, 4, 5, or 6 R ^G1 substituents.

In some embodiments, R ^5A11 is 5- to 6-membered monocyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, which is independently optionally substituted with 1 or 2 R ^G1 substituents.

In some embodiments, R ^5A11 is pyridyl, which is optionally substituted with 1 or 2 independently selected R ^G1 substituents.

In some embodiments of Formula I, each R ^5AG is independently halogen, -CN, -OR, - SR, -N(R) ₂ , -C(O)R, -C(O)OR, -C(O)N(R) ₂ , -C(O)NR(OR), -OC(O)R, -OC(O)N(R) ₂ , - OC(O)OR, -OSO2R, -OSO ₂ N(R) ₂ , -N(R)C(O)R, -NRC(O)OR, -NRC(O)N(R) ₂ , -N(R)SO ₂ R, - NRS(O) ₂ N(R) ₂ , -NROR, -NRS(O)R, -NRS(O)N(R) ₂ , -S(O)R, -SO2R, -S(O)N(R) ₂ , -SO ₂ N(R) ₂ , -SO3R, -C(=NR ^m )R, -C(=NR ^m )N(R) ₂ , -NRC(=NR ^m )R, -NRC(=NR ^m )N(R) ₂ , - NRS(O)(=NR ^m )R, -NRS(O)(=NR ^m )N(R) ₂ , -OS(O)(=NR ^m )R, -S(O)(=NR ^m )R, - S(0)(=NR ^m )N(R)2, -P(O)(R) ₂ , or an optionally substituted group selected from C1-6 aliphatic, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 10- membered saturated or partially unsaturated bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1-3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 10- membered saturated or partially unsaturated bicyclic heterocyclyl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 6-membered monocyclic heteroaryl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, and 8- to 10-membered bicyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur.

In some embodiments of any of Formulae I, II, III, IV, V, Va, VI, Via, VII, Vila, and Vllb, each R ^G1 is independently halogen, -CN, -OR, -SR, -N(R) ₂ , -C(O)R, -C(O)OR, - C(O)N(R) ₂ , -C(O)NR(OR), -OC(O)R, -OC(O)N(R) ₂ , -OC(O)OR, -OSO ₂ R, -OSO ₂ N(R) ₂ , - N(R)C(O)R, -NRC(O)OR, -NRC(O)N(R) ₂ , -N(R)SO ₂ R, -NRS(O) ₂ N(R) ₂ , -NROR, - NRS(O)R, -NRS(O)N(R) ₂ , -S(O)R, -SO ₂ R, -S(O)N(R) ₂ , -SO ₂ N(R) ₂ , -SO ₃ R, -C(=NR ^m )R, - C(=NR ^m )N(R) ₂ , -NRC(=NR ^m )R, -NRC(=NR ^m )N(R) ₂ , -NRS(O)(=NR ^m )R, - NRS(O)(=NR ^m )N(R) ₂ , -OS(O)(=NR ^m )R, -S(O)(=NR ^m )R, -S(O)(=NR ^m )N(R) ₂ , -P(O)(R) ₂ , or an optionally substituted group selected from C1-6 aliphatic, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 6-membered monocyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, and 8- to 10- membered bicyclic heteroaryl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur.

In some embodiments of any of Formulae I, II, III, IV, V, Va, VI, and Via, each R ^G1 is independently halogen, -CN, -OR, -SR, -N(R) ₂ , -C(O)R, -C(O)OR, -C(O)N(R) ₂ , - C(O)NR(OR), -OC(O)R, -OC(O)N(R) ₂ , -OC(O)OR, -OSO ₂ R, -OSO ₂ N(R) ₂ , -N(R)C(O)R, - NRC(O)OR, -NRC(O)N(R) ₂ , -N(R)SO ₂ R, -NRS(O) ₂ N(R) ₂ , -NROR, -NRS(O)R, - NRS(O)N(R) ₂ , -S(O)R, -SO ₂ R, -S(O)N(R) ₂ , -SO ₂ N(R) ₂ , -SO ₃ R, -C(=NR ^m )R, - C(=NR ^m )N(R) ₂ , -NRC(=NR ^m )R, -NRC(=NR ^m )N(R) ₂ , -NRS(O)(=NR ^m )R, - NRS(O)(=NR ^m )N(R) ₂ , -OS(O)(=NR ^m )R, -S(O)(=NR ^m )R, -S(O)(=NR ^m )N(R) ₂ , -P(O)(R) ₂ , or an optionally substituted group selected from C1-6 aliphatic, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 6-membered monocyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, and 8- to 10- membered bicyclic heteroaryl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur.

In some embodiments of any of Formulae I, II, and III, each R ^G1 is independently halogen, -CN, -OR, -SR, -N(R) ₂ , -C(O)R, -C(O)OR, -C(O)N(R) ₂ , -C(O)NR(OR), -OC(O)R, - OC(O)N(R) ₂ , -OC(O)OR, -OSO ₂ R, -OSO ₂ N(R) ₂ , -N(R)C(O)R, -NRC(O)OR, -NRC(O)N(R) ₂ , -N(R)SO ₂ R, -NRS(O) ₂ N(R) ₂ , -NROR, -NRS(O)R, -NRS(O)N(R) ₂ , -S(O)R, -SO ₂ R, - S(O)N(R) ₂ , -SO ₂ N(R) ₂ , -SO ₃ R, -C(=NR ^m )R, -C(=NR ^m )N(R) ₂ , -NRC(=NR ^m )R, - NRC(=NR ^m )N(R) ₂ , -NRS(O)(=NR ^m )R, -NRS(O)(=NR ^m )N(R) ₂ , -OS(O)(=NR ^m )R, - S(O)(=NR ^m )R, -S(O)(=NR ^m )N(R) ₂ , -P(O)(R) ₂ , or an optionally substituted group selected from C1-6 aliphatic, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 6- membered monocyclic heteroaryl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, and 8- to 10-membered bicyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur.

In some embodiments, each R ^G1 is independently selected from halogen, C1-6 alkyl, phenyl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1-3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 10- membered saturated or partially unsaturated bicyclic heterocyclyl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 6-membered monocyclic heteroaryl, having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 8- to 10-membered bicyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, -C(O)OR, -C(O)N(R) ₂ , and -C(O)NR(OR), wherein each Ci-6 alkyl, phenyl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl, 5- to 6-membered monocyclic heteroaryl, and 8- to 10-membered bicyclic heteroaryl is optionally substituted by 1, 2, 3, or 4 independently selected R ^AG1 substituents.

In some embodiments, each R ^G1 is independently selected from halogen, C1-6 alkyl, phenyl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1-3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 10- membered saturated or partially unsaturated bicyclic heterocyclyl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 6-membered monocyclic heteroaryl, having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 8- to 10-membered bicyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, -C(O)OR, -C(0)N(R)2, and -C(O)NR(OR), wherein each Ci-6 alkyl, phenyl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl, 5- to 6-membered monocyclic heteroaryl, and 8- to 10-membered bicyclic heteroaryl is optionally substituted by 1 or 2 independently selected R ^AG1 substituents.

In some embodiments, each R ^G1 is independently selected from halogen, C1-6 alkyl, phenyl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1-3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 10- membered saturated or partially unsaturated bicyclic heterocyclyl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 6-membered monocyclic heteroaryl, having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, -C(O)OR, -C(0)N(R)2, and -C(O)NR(OR), wherein each C1-6 alkyl, phenyl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl, 5- to 6-membered monocyclic heteroaryl, is optionally substituted by 1, 2, 3, or 4 independently selected R ^AG1 substituents.

In some embodiments, each R ^AG1 is independently selected from halogen, -(CFbk- ₄ R°, -(CH ₂ ) ₀ -4OR ^O , -(CH ₂ ) ₀ _4C(O)NR ^O 2, -(CH ₂ ) ₀ ^C(O)OR ^O , -(CH ₂ ) ₀ -4C(O)R ^O , -0(CH ₂ )O-4R°, CN, -(CH ₂ ) ₀ -4C(O)NR°2, and -C(O)N(OR°)R°, wherein each R° is independently hydrogen, C1-6 alkyl, or 3- to 6-membered saturated ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur.

In some embodiments, each R ^AG1 is independently selected from halogen, -(CIDo- ₄ R°, -(CH ₂ ) ₀ -4OR ^O , -(CH ₂ ) ₀ _4C(O)NR ^O 2, -(CH ₂ ) ₀ ^C(O)OR ^O , -(CH ₂ ) ₀ -4C(O)R ^O , and -C(O)N(OR°)R°. In some embodiments, each R° is independently hydrogen or C1-6 alkyl. In some embodiments, each R° is independently hydrogen or C1-3 alkyl. In some embodiments, each R° is independently hydrogen or methyl.

In some embodiments, each R ^AG1 is independently selected from halogen, -(CH ₂ )o- 4R ⁰ , -(CH ₂ ) ₀ _4OR°, -(CH ₂ ) _0-4 C(O)NR ^O ₂ , -(CH ₂ ) ₀ ^C(O)OR ^O , -(CH ₂ ) _0-4 C(O)R ^O , and -C(O)N(OR°)R°, wherein each R° is independently hydrogen or C1-6 alkyl.

In some embodiments, each R ^AG1 is independently selected from fluoro, methyl, ethyl, hydroxyethyl, -COOH, methylaminocarbonyl, dimethylaminocarbonyl, methylcarbonyl, methoxyaminocarbonyl, cyano, cyclopropyl, hydroxy, methoxy, dimethylamino, dimethylaminomethyl, and tetrahydropyranyl.

In some embodiments, each R ^AG1 is independently selected from fluoro, methyl, hydroxyethyl, -COOH, methylaminocarbonyl, dimethylaminocarbonyl, methylcarbonyl, and methoxyaminocarbonyl .

In some embodiments, each R ^G1 is independently selected from halogen, C1-6 aliphatic, and -C(O)OR. In some embodiments, each R ^G1 is independently selected from halogen, C1-6 alkyl, and -C(O)OR. In some embodiments, each R ^G1 is independently selected from halogen, C1-3 alkyl, and -C(O)OR.In some embodiments, each R ^G1 is independently selected from halogen, C1-6 alkyl, and -C(O)OH. In some embodiments, each R ^G1 is independently selected from halogen, C1-3 alkyl, and -C(O)OH. In some embodiments, each R ^G1 is independently selected from fluoro, chloro, methyl, and -C(O)OH.

In some embodiments, each R ^G1 is independently selected from methyl, chloro, pyridinyl, pyridinonyl, phenyl, pyrazolyl, pyrimidinyl, piperazinyl, 5, 6, 7, 8 -tetrahydrof l, 2, 4]triazolo [4,3 -a]pyrazinyl, l,2,4-oxadiazol-5(4H)-onyl, tetrazolyl, triazolyl, oxadiazolyl, indazolyl, quinolinyl, piperidinyl, piperazinyl, morpholinyl, isoindolinonyl, hexahydro-3H- oxazolo[3,4-a]pyrazin-3-onyl, 4,5,6,7-tetrahydropyrazolo[l,5-a]pyrazinyl, -C(O)OR, - C(O)N(R) ₂ , and -C(O)NR(OR), wherein the pyridinyl, pyridinonyl, phenyl, pyrazolyl, pyrimidinyl, piperazinyl, 5,6,7,8-tetrahydro-[l,2,4]triazolo[4,3-a]pyrazinyl, 1,2,4-oxadiazol- 5(4H)-onyl, tetrazolyl, triazolyl, oxadiazolyl, indazolyl, quinolinyl, piperidinyl, piperazinyl, morpholinyl, isoindolinonyl, hexahydro-3H-oxazolo[3,4-a]pyrazin-3-onyl, 4, 5,6,7- tetrahydropyrazolo[l,5-a]pyrazinyl, are each optionally substituted by 1 or 2 R ^AG1 substituents.

In some embodiments, each R ^G1 is independently selected from methyl, chloro, pyridinyl, pyridinonyl, phenyl, pyrazolyl, pyrimidinyl, piperazinyl, 5,6,7,8-tetrahydro- [l,2,4]triazolo[4,3-a]pyrazinyl, l,2,4-oxadiazol-5(4H)-onyl, tetrazolyl, triazolyl, oxadiazolyl, indazolyl, quinolinyl, piperidinyl, piperazinyl, morpholinyl, isoindolinonyl, hexahydro-3H- oxazolo[3,4-a]pyrazin-3-onyl, 4,5,6,7-tetrahydropyrazolo[l,5-a]pyrazinyl, -C(O)OR, - C(0)N(R)2, and -C(O)NR(OR), wherein the pyridinyl, pyridinonyl, phenyl, pyrazolyl, pyrimidinyl, piperazinyl, 5,6,7,8-tetrahydro-[l,2,4]triazolo[4,3-a]pyrazinyl, 1,2,4-oxadiazol- 5(4H)-onyl, tetrazolyl, triazolyl, oxadiazolyl, indazolyl, quinolinyl, piperidinyl, piperazinyl, morpholinyl, isoindolinonyl, hexahydro-3H-oxazolo[3,4-a]pyrazin-3-onyl, 4, 5,6,7- tetrahydropyrazolo[l,5-a]pyrazinyl, are each optionally substituted by 1 or 2 R ^AG1 substituents independently selected from fluoro, methyl, ethyl, hydroxyethyl, -COOH, methylaminocarbonyl, dimethylaminocarbonyl, methylcarbonyl, methoxyaminocarbonyl, cyano, cyclopropyl, hydroxy, methoxy, dimethylamino, dimethylaminomethyl, and tetrahydropyranyl .

In some embodiments, each R ^G1 is independently selected from methyl, chloro, pyridinyl, pyridinonyl, phenyl, pyrazolyl, pyrimidinyl, piperazinyl, 5, 6, 7, 8 -tetrahydrof l, 2, 4]triazolo [4,3 -a]pyrazinyl, 1, 2, 4-oxadiazol-5(4H)-onyl, tetrazolyl, -C(O)OR, - C(0)N(R)2, and -C(O)NR(OR), wherein the pyridinyl, pyridinonyl, phenyl, pyrazolyl, pyrimidinyl, piperazinyl, 5,6,7,8-tetrahydro-[l,2,4]triazolo[4,3-a]pyrazinyl, 1,2,4-oxadiazol- 5(4H)-onyl, and tetrazolyl, are each optionally substituted by 1 or 2 R ^AG1 substituents independently selected from fluoro, methyl, hydroxyethyl, -COOH, methylaminocarbonyl, dimethylaminocarbonyl, methylcarbonyl, and methoxyaminocarbonyl.

In some embodiments, R ^G1 is -C(O)OR. In some embodiments, R ^G1 is -C(O)OH. In some embodiments, one R ^G1 is -C(O)OR and a second R ^G1 is selected from halogen and Ci-6 aliphatic. In some embodiments, one R ^G1 is -C(O)OR and a second R ^G1 is selected from halogen and C1-6 alkyl. In some embodiments, one R ^G1 is -C(O)OR and a second R ^G1 is selected from halogen and C1-3 alkyl. In some embodiments, one R ^G1 is -C(O)OH and a second R ^G1 is selected from halogen and C1-6 alkyl. In some embodiments, one R ^G1 is - C(O)OH and a second R ^G1 is selected from halogen and C1-3 alkyl. In some embodiments, one R ^G1 is -C(O)OH and a second R ^G1 is selected from fluoro, chloro, and methyl.

In some embodiments of any of Formulae I, II, III, IV, V, Va, VI, Via, VII, Vila, and Vllb each R is independently hydrogen or an optionally substituted group selected from C1-6 aliphatic, 3- to 7-membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic carbocyclyl, phenyl, 8- to 10- membered bicyclic aryl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 6-membered monocyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, and 8- to 10-membered bicyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur; or two R when attached to the same nitrogen atom are taken together to form an optionally substituted ring selected from 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 0-2 additional heteroatoms independently selected from nitrogen, oxygen, and sulfur, and 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 0-3 additional heteroatoms independently selected from nitrogen, oxygen, and sulfur.

In some embodiments of any of Formulae I, II, III, IV, V, Va, VI, and Via each R is independently hydrogen or an optionally substituted group selected from C1-6 aliphatic, 3- to 7 -membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1-3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5 - to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 6-membered monocyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, and 8- to 10- membered bicyclic heteroaryl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur; or two R when attached to the same nitrogen atom are taken together to form an optionally substituted ring selected from 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 0-2 additional heteroatoms independently selected from nitrogen, oxygen, and sulfur, and 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 0-3 additional heteroatoms independently selected from nitrogen, oxygen, and sulfur.

As described above, in some embodiments of any of Formulae I, II, and III, each R is independently hydrogen or an optionally substituted group selected from C1-6 aliphatic, 3- to 7 -membered saturated or partially unsaturated monocyclic carbocyclyl, 5- to 10-membered saturated or partially unsaturated bicyclic carbocyclyl, phenyl, 8- to 10-membered bicyclic aryl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1-3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, 5- to 6-membered monocyclic heteroaryl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, and 8- to 10- membered bicyclic heteroaryl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur; or two R when attached to the same nitrogen atom are taken together to form an optionally substituted ring selected from 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 0-2 additional heteroatoms independently selected from nitrogen, oxygen, and sulfur, and 5- to 10-membered saturated or partially unsaturated bicyclic heterocyclyl having 0-3 additional heteroatoms independently selected from nitrogen, oxygen, and sulfur.

In some embodiments, each R is independently selected from hydrogen, C1-6 alkyl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1-3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, and 5- to 6-membered monocyclic heteroaryl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein each 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl and 5- to 6-membered monocyclic heteroaryl is optionally substituted by Ci-6 alkyl.

In some embodiments, each R is independently selected from hydrogen, methyl, methylpiperidinyl, and pyridinyl.

In some embodiments, each R is independently selected from hydrogen, methyl, methylpiperidinyl, and pyridinyl. In some embodiments, R is hydrogen.

In some embodiments: each R ^G1 is independently selected from methyl, chloro, pyridinyl, pyridinonyl, phenyl, pyrazolyl, pyrimidinyl, piperazinyl, 5,6,7,8-tetrahydro-[l,2,4]triazolo[4,3-a]pyrazinyl, l,2,4-oxadiazol-5(4H)-onyl, tetrazolyl, triazolyl, oxadiazolyl, indazolyl, quinolinyl, piperidinyl, piperazinyl, morpholinyl, isoindolinonyl, hexahydro-3H-oxazolo[3,4-a]pyrazin-3- onyl, 4,5,6,7-tetrahydropyrazolo[l,5-a]pyrazinyl, -C(O)OR, -C(O)N(R)2, and -C(O)NR(OR), wherein the pyridinyl, pyridinonyl, phenyl, pyrazolyl, pyrimidinyl, piperazinyl, 5, 6,7,8- tetrahydro-[l,2,4]triazolo[4,3-a]pyrazinyl, l,2,4-oxadiazol-5(4H)-onyl, tetrazolyl, triazolyl, oxadiazolyl, indazolyl, quinolinyl, piperidinyl, piperazinyl, morpholinyl, isoindolinonyl, hexahydro-3H-oxazolo [3 ,4-a]pyrazin-3 -onyl, 4,5 ,6,7-tetrahydropyrazolo [ 1 ,5 -a]pyrazinyl, are each optionally substituted by 1 or 2 R ^AG1 substituents independently selected from fluoro, methyl, ethyl, hydroxyethyl, -COOH, methylaminocarbonyl, dimethylaminocarbonyl, methylcarbonyl, methoxyaminocarbonyl, cyano, cyclopropyl, hydroxy, methoxy, dimethylamino, dimethylaminomethyl, and tetrahydropyranyl; and each R is independently selected from hydrogen, methyl, methylpiperidinyl, and pyridinyl

In some embodiments: each R ^G1 is independently selected from methyl, chloro, pyridinyl, pyridinonyl, phenyl, pyrazolyl, pyrimidinyl, piperazinyl, 5,6,7,8-tetrahydro-[l,2,4]triazolo[4,3-a]pyrazinyl, l,2,4-oxadiazol-5(4H)-onyl, tetrazolyl, -C(O)OR, -C(O)N(R)2, and -C(O)NR(OR), wherein the pyridinyl, pyridinonyl, phenyl, pyrazolyl, pyrimidinyl, piperazinyl, 5,6,7,8-tetrahydro- [l,2,4]triazolo[4,3-a]pyrazinyl, l,2,4-oxadiazol-5(4H)-onyl, and tetrazolyl, are each optionally substituted by 1 or 2 R ^AG1 substituents independently selected from fluoro, methyl, hydroxyethyl, -COOH, methylaminocarbonyl, dimethylaminocarbonyl, methylcarbonyl, and methoxyaminocarbonyl; and each R is independently selected from hydrogen, C1-6 alkyl, 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl having 1 -3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, and 5- to 6-membered monocyclic heteroaryl having 1 -4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein each 3- to 7-membered saturated or partially unsaturated monocyclic heterocyclyl and 5- to 6-membered monocyclic heteroaryl is optionally substituted by C1-6 alkyl.

In some embodiments: each R ^G1 is independently selected from methyl, chloro, pyridinyl, pyridinonyl, phenyl, pyrazolyl, pyrimidinyl, piperazinyl, 5,6,7,8-tetrahydro-[l,2,4]triazolo[4,3-a]pyrazinyl,

I,2,4-oxadiazol-5(4H)-onyl, tetrazolyl, -C(O)OR, -C(0)N(R)2, and -C(O)NR(OR), wherein the pyridinyl, pyridinonyl, phenyl, pyrazolyl, pyrimidinyl, piperazinyl, 5,6,7,8-tetrahydro- [l,2,4]triazolo[4,3-a]pyrazinyl, l,2,4-oxadiazol-5(4H)-onyl, and tetrazolyl, are each optionally substituted by 1 or 2 R ^AG1 substituents independently selected from fluoro, methyl, hydroxyethyl, -COOH, methylaminocarbonyl, dimethylaminocarbonyl, methylcarbonyl, and methoxyaminocarbonyl; and each R is independently selected from hydrogen, methyl, methylpiperidinyl, and pyridinyl.

In some embodiments of any of Formulae I, II, III, IV, V, Va, VI, Via, VII, Vila, and Vllb, each R ^m is independently -OH, -CN, or R. In some embodiments of any of Formulae I,

II, III, IV, V, Va, VI, and Via each R ^m is independently -OH, -CN, or R. As described above, in some embodiments of any of Formulae I, II, and III, each R ^m is independently -OH, -CN, or R.

In some embodiments of any of Formulae I, II, III, IV, V, Va, VI, Via, VII, Vila, and Vllb, each n is 0, 1, 2, 3, or 4. In some embodiments of any of Formulae I, II, III, IV, V, Va, VI, and Via, each n is 0, 1, 2, 3, or 4. As described above, in some embodiments of any of Formulae I, II, and III, each n is 0, 1, 2, 3, or 4. In some embodiments, n is 0. In some embodiments, n is 1, 2, 3, or 4. In some embodiments, n is 0 or 1. In some embodiments, n is 1.

In some embodiments, the compound of Formula I is a compound of Formula V : or a pharmaceutically acceptable salt thereof, wherein z is 0, 1, 2, or 3, and each of U, Z, Ring A, R ³ , R ⁵ , and R ^AG are as defined above for Formula I and described in classes and subclasses herein, both singly and in combination.

In some embodiments, the compound of Formula I is a compound of Formula Va: or a pharmaceutically acceptable salt thereof, wherein z is 0, 1, 2, or 3, and each of U, Z, Ring A, Ring B, R ³ , L ⁵ , R ^G1 , R ^AG , and m are as defined above for Formula I and described in classes and subclasses herein, both singly and in combination.

In some embodiments, the compound of Formula I is a compound of Formula VI: or a pharmaceutically acceptable salt thereof, wherein each of U, Z, R ¹ , R ³ , R ^5A , and R ^AG are as defined above for Formula I and described in classes and subclasses herein, both singly and in combination. In some embodiments, the compound of Formula I is a compound of Formula Via:

Via or a pharmaceutically acceptable salt thereof, wherein each of U, Z, R ¹ , R ³ , R ^5A12 , and R ^AG are as defined above for Formula I and described in classes and subclasses herein, both singly and in combination.

In some embodiments, the compound is of Formula VII: or a pharmaceutically acceptable salt thereof, wherein z is 0, 1, 2, or 3, and each of U, Z, R ³ , R ⁵ , and R ^AG are as defined above for Formula I and described in classes and subclasses herein, both singly and in combination.

In some embodiments, the compound is of Formula Vila:

Vila or a pharmaceutically acceptable salt thereof, wherein z is 0, 1, 2, or 3, and each of U, Z, R ³ , L ⁵ , R ^5A12 , and R ^AG are as defined above for Formula I and described in classes and subclasses herein, both singly and in combination.

In some embodiments, the compound is of Formula Vllb: or a pharmaceutically acceptable salt thereof, wherein z is 0, 1, 2, or 3, and each of U, Z, Ring B, R ³ , L ⁵ , R ^G1 , R ^AG , and m are as defined above for Formula I and described in classes and subclasses herein, both singly and in combination.

In some embodiments, the present disclosure provides compounds selected from Table 1, or a pharmaceutically acceptable salt thereof.

In some embodiments, the compound provided herein is selected from:

2-((l-(4,7-dimethyl-5-oxo-4,5-dihydropyrazolo[l,5-a]quina zolin-9- yl)ethyl)amino)benzoic acid;

2-(( 1 -(4,7-dimethyl-5 -oxo-3 -phenyl -4, 5 -dihydropyrazolo [1,5 -a]quinazolin-9- yl)ethyl)amino)benzoic acid;

2-((l-(4,7-dimethyl-5-oxo-3-vinyl-4,5-dihydropyrazolo[l,5 -a]quinazolin-9- yl)ethyl)amino)benzoic acid;

2-((l-(4,7-dimethyl-5-oxo-4,5-dihydroimidazo[l,5-a]quinaz olin-9- yl)ethyl)amino)benzoic acid;

2-((l-(3-ethyl-4,7-dimethyl-5-oxo-4,5-dihydroimidazo[l,5- a]quinazolin-9- yl)ethyl)amino)benzoic acid; 2-((l-(4,7-dimethyl-5-oxo-3-(pyridin-2-yl)-4,5-dihydroimidaz o[l,5-a]quinazolin-9- yl)ethyl)amino)benzoic acid;

2-((l-(3,4,7-trimethyl-5-oxo-4,5-dihydroimidazo[l,5-a]qui nazolin-9- yl)ethyl)amino)benzoic acid;

6-methyl-3 -(( 1 -(3 ,4,7-trimethyl-5 -oxo-4,5 -dihydroimidazo [ 1 ,5 -a] quinazolin-9- yl)ethyl)amino)picolinic acid;

2-((l-(7-chloro-3-ethyl-4-methyl-5-oxo-4,5-dihydroimidazo [l,5-a]quinazolin-9- yl)ethyl)amino)benzoic acid;

2-(( 1 -(7 -chloro-4-methyl-5 -oxo-4,5 -dihydroimidazo [ 1 ,5 -a] quinazolin-9- yl)ethyl)amino)benzoic acid;

2-((l-(7 -chloro-3 ,4 -dimethyl -5 -oxo -4,5 -dihydroimidazo [ 1 , 5 -a] quinazolin-9 - yl)ethyl)amino)benzoic acid;

2-(( 1 -(7 -chloro-4-ethyl-5 -oxo-4,5 -dihydroimidazo [ 1 ,5 -a] quinazolin-9- yl)ethyl)amino)benzoic acid;

2-(( 1 -(7 -chloro-3 ,4-diethyl-5 -oxo-4,5 -dihydroimidazo [ 1 ,5 -a] quinazolin-9- yl)ethyl)amino)benzoic acid;

2-((l-(4,7-dimethyl-5-oxo-4,5-dihydroimidazo[l,2-a]quinaz olin-9- yl)ethyl)amino)benzoic acid;

2-((l-(4,7-dimethyl-5-oxo-4,5-dihydro-[l,2,4]triazolo[l,5 -a]quinazolin-9- yl)ethyl)amino)benzoic acid;

2-((l-(3,4,7-trimethyl-5-oxo-4,5-dihydro-3H-pyrazolo[3,4- c]isoquinolin-9- yl)ethyl)amino)benzoic acid;

6-chloro-3-((l-(3,4,7-trimethyl-5-oxo-4,5-dihydro-3H-pyra zolo[3,4-c]isoquinolin-9- yl)ethyl)amino)picolinic acid;

2-(( 1 -(3 -ethyl-4,7-dimethyl-5 -oxo-4,5 -dihydro-3//-py razolo [3 ,4-c] isoquinolin-9- yl)ethyl)amino)benzoic acid;

6-chloro-3 -((1 -(3 -ethyl -4, 7 -dimethyl -5 -oxo-4,5 -dihydro-3H-pyrazolo [3 ,4- c]isoquinolin-9-yl)ethyl)amino)picolinic acid;

3 -(( 1 -(3 -ethyl-4,7-dimethyl-5 -oxo-4,5 -dihydro-3//-py razolo [3 ,4-c] isoquinolin-9- yl)ethyl)amino)-6-methylpicolinic acid;

2-((l-(4-ethyl-3,7-dimethyl-5-oxo-4,5-dihydro-3H-pyrazolo [3,4-c]isoquinolin-9- yl)ethyl)amino)benzoic acid;

6-chloro-3 -((1 -(4-ethyl-3 ,7 -dimethyl -5 -oxo-4,5 -dihydro-3H-pyrazolo [3 ,4- c]isoquinolin-9-yl)ethyl)amino)picolinic acid; 2-(( 1 -(3 ,4-diethyl-7 -methyl-5 -oxo-4,5 -dihydro-3//-py razolo [3 ,4-c] isoquinolin-9- yl)ethyl)amino)benzoic acid;

2-(( 1 -(3 ,4-diethyl-7 -methyl-5 -oxo-4,5 -dihydro-3//-py razolo [3 ,4-c] isoquinolin-9- yl)ethyl)amino) -5 -fluorobenzoic acid;

3 -(( 1 -(3 ,4-diethyl-7 -methyl-5 -oxo-4,5 -dihydro-3//-py razolo [3 ,4-c] isoquinolin-9- yl)ethyl)amino)-6-methylpicolinic acid;

2-((l-(4,7-dimethyl-5-oxo-2-propyl-4,5-dihydro-2H-pyrazol o[3,4-c]isoquinolin-9- yl)ethyl)amino)benzoic acid;

2-((l-(4,7-dimethyl-5-oxo-2-(tetrahydro-2H-pyran-4-yl)-4, 5-dihydro-2H- pyrazolo[3,4-c]isoquinolin-9-yl)ethyl)amino)benzoic acid;

2-(( l-(4,7-dimethyl-5-oxo-2-phenethyl-4,5-dihydro-2H-pyrazolo[3, 4-c]isoquinolin-9- yl)ethyl)amino)benzoic acid;

2-((l-(2-(2-fluoropyridin-4-yl)-4,7-dimethyl-5-oxo-4,5-di hydro-2H-pyrazolo[3,4- c]isoquinolin-9-yl)ethyl)amino)benzoic acid;

2-((l-(4,7-dimethyl-5-oxo-2-(pyrimidin-5-yl)-4,5-dihydro- 2H-pyrazolo[3,4- c]isoquinolin-9-yl)ethyl)amino)benzoic acid;

6-chloro-3 -(( 1 -(4,7 -dimethyl -5 -oxo-2-(o-tolyl)-4,5 -dihydro-2H-pyrazolo[3,4- c]isoquinolin-9-yl)ethyl)amino)picolinic acid;

2-[l-(3-methoxy-5,8-dimethyl-6-oxo-benzo[c][l,8]naphthyri din-10- yl)ethylamino] benzoic acid;

2-[l-[3-(dimethylamino)-5,8-dimethyl-6-oxo-benzo[c][l,8]n aphthyridin-10- yl] ethylamino] benzoic acid;

2-[l-[3-(2-methoxyethoxy)-5,8-dimethyl-6-oxo-benzo[c][l,8 ]naphthyridin-10- yl] ethylamino] benzoic acid;

2-((l-(7-chloro-3,4-dimethyl-5-oxo-4,5-dihydro-3H-pyrazol o[3,4-c]isoquinolin-9- yl)ethyl)amino)benzoic acid;

2-((l-(8-methyl-6-oxo-4,5-dihydro-3H,6H-2,2a,5a-triazaace anthrylen-10- yl)ethyl)amino)benzoic acid;

6-methyl-3-((l-(8-methyl-6-oxo-4,5-dihydro-3H,6H-2,2a,5a- triazaaceanthrylen-10- yl)ethyl)amino)picolinic acid;

2-((l-(3,8-dimethyl-6-oxo-4,5-dihydro-3H,6H-2,2a,5a-triaz aaceanthrylen-10- yl)ethyl)amino)benzoic acid;

6-chloro-3-((l-(3,8-dimethyl-6-oxo-4,5-dihydro-3H,6H-2,2a ,5a-triazaaceanthrylen- 10-yl)ethyl)amino)picolinic acid; 2-ethyl-3,6-dimethyl-8-((6-methyl-[2,4'-bipyridin]-3-yl)amin o)-8,9- dihydrobenzo [de]pyrazolo [4,5 , 1 -ij ] [ 1 ,7]naphthyridin-4(3H)-one ;

9-( I -((6-chloro-l'-mcthyl-6'-oxo-l'.6'-dihydro-|2.3'-bipyridin|- 3-yl)amino)cthyl)-3- ethyl-4,7-dimethyl-3,4-dihydro-5H-pyrazolo[3,4-c]isoquinolin -5-one;

4-(6-chloro-3-((l-(3-ethyl-4,7-dimethyl-5-oxo-4,5-dihydro -3H-pyrazolo[3,4- c]isoquinolin-9-yl)ethyl)amino)pyridin-2-yl)-2-fluoro-N-meth ylbenzamide;

9-(l-((6-chloro-2-(pyrimidin-4-yl)pyridin-3-yl)amino)ethy l)-3-ethyl-4,7-dimethyl-

3 ,4-dihydro -5H-pyrazolo [3,4 -c] isoquinolin-5 -one ;

9-(l-((6-chloro-2-(pyrimidin-5-yl)pyridin-3-yl)amino)ethy l)-3-ethyl-4,7-dimethyl-

3 ,4-dihydro -5H-pyrazolo [3,4 -c] isoquinolin-5 -one ;

9-(l-((6-chloro-3'-fluoro-[2,4'-bipyridin]-3-yl)amino)eth yl)-3-ethyl-4,7-dimethyl-3,4- dihydro-5H-pyrazolo [3 ,4-c]isoquinolin-5 -one ;

4-(6-chloro-3-((l-(3-ethyl-4,7-dimethyl-5-oxo-4,5-dihydro -3H-pyrazolo[3,4- c] isoquinolin -9 -yl)ethyl)amino)pyridin-2 -yl) -N,N -dimethylbenzamide ;

4-(6-chloro-3-((l-(3-ethyl-4,7-dimethyl-5-oxo-4,5-dihydro -3H-pyrazolo[3,4- c]isoquinolin-9-yl)ethyl)amino)pyridin-2-yl)benzoic acid;

9-(l-((6-chloro-2-(l-methyl-lH-pyrazol-5-yl)pyridin-3-yl) amino)ethyl)-3-ethyl-4,7- dimethyl-3 ,4 -dihydro -5H-pyrazolo [3,4 -c] isoquinolin-5 -one ;

9-( 1 -((6-chloro-2-( 1 ,5-dimethyl- lH-pyrazol-4-yl)pyridin-3 -yl)amino)ethyl)-3 -ethyl - 4,7-dimethyl-3,4-dihydro-5H-pyrazolo[3,4-c]isoquinolin-5-one ;

9-(l-((6-chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3-yl) amino)ethyl)-3-ethyl-4,7- dimethyl-3 ,4 -dihydro -5H-pyrazolo [3,4 -c] isoquinolin-5 -one ;

9-(l-((6-chloro-2-(lH-pyrazol-4-yl)pyridin-3-yl)amino)eth yl)-3-ethyl-4,7-dimethyl-

3.4-dihydro -5H-pyrazolo [3,4 -c] isoquinolin-5 -one ;

3 -ethyl -4, 7 -dimethyl-9-( 1 -((6-methyl-2-( 1 -methyl- lH-pyrazol-4-yl)pyridin-3 - yl)amino)ethyl)-3,4-dihydro-5H-pyrazolo[3,4-c]isoquinolin-5- one;

3-ethyl-4,7 -dimethyl-9-( 1 -((6-methyl-2-( lH-pyrazol-4-yl)pyridin-3 -yl)amino)ethyl)-

3.4-dihydro-5H-pyrazolo[3,4-c]isoquinolin-5-one;

3-ethyl-4,7-dimethyl-9-(l-((6-methyl-2-(l-methyl-lH-pyraz ol-5-yl)pyridin-3- yl)amino)ethyl)-3,4-dihydro-5H-pyrazolo[3,4-c]isoquinolin-5- one;

4-(3-((l-(3-ethyl-4,7-dimethyl-5-oxo-4,5-dihydro-3H-pyraz olo[3,4-c]isoquinolin-9- yl)ethyl)amino)-6-methylpyridin-2-yl)-2-fluoro-N-methylbenza mide;

3-ethyl-9-(l-((2-(l-(2-hydroxyethyl)-lH-pyrazol-4-yl)phen yl)amino)ethyl)-4,7- dimethyl-3 ,4 -dihydro -5H-py razol o [3,4 -c] isoquinolin-5 -one ; 9-( 1 -((2-(4-acetylpiperazin- 1 -yl)phenyl)amino)ethyl)-3-ethyl-4,7 -dimethyl-3,4- dihydro-5//-py razolo 13 ,4-c | i soquinol i n-5 -one ;

9-(l-((2-(5,6-dihydro-[l,2,4]triazolo[4,3-a]pyrazin-7(8H) -yl)phenyl)amino)ethyl)-3- ethyl-4,7-dimethyl-3,4-dihydro-5H-pyrazolo[3,4-c]isoquinolin -5-one;

3-(6-chloro-3-((l-(3-ethyl-4,7-dimethyl-5-oxo-4,5-dihydro -3H-pyrazolo[3,4- c]isoquinolin-9-yl)ethyl)amino)pyridin-2-yl)-l,2,4-oxadiazol -5(4H)-one;

3-(6-chloro-3-((l-(4-(2,2-difluoroethyl)-3-ethyl-7-methyl -5-oxo-4,5-dihydro-3H- pyrazolo[3,4-c]isoquinolin-9-yl)ethyl)amino)pyridin-2-yl)-l, 2,4-oxadiazol-5(4H)-one;

3 -(6-chloro-3 -(( 1 -(3 -cyclopentyl -4, 7-dimethyl-5 -oxo-4,5 -dihydro-3H-pyrazolo [3,4- c]isoquinolin-9-yl)ethyl)amino)pyridin-2-yl)-l,2,4-oxadiazol -5(4H)-one;

3-(6-chloro-3-((l-(3-isopropyl-4,7-dimethyl-5-oxo-4,5-dih ydro-3H-pyrazolo[3,4- c]isoquinolin-9-yl)ethyl)amino)pyridin-2-yl)-l,2,4-oxadiazol -5(4H)-one;

3-(6-chloro-3-((l-(7-chloro-3-ethyl-4-methyl-5-oxo-4,5-di hydro-3H-pyrazolo[3,4- c]isoquinolin-9-yl)ethyl)amino)pyridin-2-yl)-l,2,4-oxadiazol -5(4H)-one;

3-(6-chloro-3-((l-(7-chloro-3-(cyclopropylmethyl)-4-methy l-5-oxo-4,5-dihydro-3H- pyrazolo[3,4-c]isoquinolin-9-yl)ethyl)amino)pyridin-2-yl)-l, 2,4-oxadiazol-5(4H)-one;

3 -(6-chloro-3 -(( 1 -(4,7-dimethyl-5 -oxo-3 -propyl -4, 5 -dihydro-3H-pyrazolo [3,4- c]isoquinolin-9-yl)ethyl)amino)pyridin-2-yl)-l,2,4-oxadiazol -5(4H)-one;

3 -(6-chloro-3 -(( 1 -(4,7-dimethyl-5 -oxo-3 -(tetrahydrofuran-3 -yl)-4,5 -dihydro-3H- pyrazolo[3,4-c]isoquinolin-9-yl)ethyl)amino)pyridin-2-yl)-l, 2,4-oxadiazol-5(4H)-one;

3-(6-chloro-3-((l-(3-(2-methoxyethyl)-4,7-dimethyl-5-oxo- 4,5-dihydro-3H- pyrazolo[3,4-c]isoquinolin-9-yl)ethyl)amino)pyridin-2-yl)-l, 2,4-oxadiazol-5(4H)-one;

3-(6-chloro-3-((l-(3-(cyclopropylmethyl)-4,7-dimethyl-5-o xo-4,5-dihydro-3H- pyrazolo[3,4-c]isoquinolin-9-yl)ethyl)amino)pyridin-2-yl)-l, 2,4-oxadiazol-5(4H)-one;

3-(6-chloro-3-((l-(3-ethyl-4-methyl-5-oxo-7-(trifluoromet hyl)-4,5-dihydro-3H- pyrazolo[3,4-c]isoquinolin-9-yl)ethyl)amino)pyridin-2-yl)-l, 2,4-oxadiazol-5(4H)-one;

3-(6-chloro-3-((l-(3,8-dimethyl-6-oxo-4,5-dihydro-3H,6H-2 ,2a,5a- triazaaceanthrylen-10-yl)ethyl)amino)pyridin-2-yl)-l,2,4-oxa diazol-5(4H)-one;

3 -(6-chloro-3 -(( 1 -(3 -ethyl-4,7-dimethyl-5 -oxo-4,5 -dihydroimidazo [ 1 ,5 -a]quinazolin- 9-yl)ethyl)amino)pyridin-2-yl)-l,2,4-oxadiazol-5(4H)-one;

9-(l-((2-(lH-tetrazol-5-yl)pyridin-3-yl)amino)ethyl)-3-et hyl-4,7-dimethyl-3,4- dihydro-5H-pyrazolo [3 ,4-c]isoquinolin-5 -one ;

6-chloro-3 -((1 -(3 -ethyl -4, 7 -dimethyl -5 -oxo-4,5 -dihydro-3H-pyrazolo [3 ,4- c]isoquinolin-9-yl)ethyl)amino)-N-(l-methylpiperidin-4-yl)pi colinamide; 6-chloro-3 -((1 -(3 -ethyl -4, 7 -dimethyl -5 -oxo-4,5 -dihydro-3H-pyrazolo [3 ,4- c] isoquinolin-9 -yl)ethyl)amino) -N -(pyridin-4 -yl)picolinamide ;

6-chloro-3 -((1 -(3 -ethyl -4, 7 -dimethyl -5 -oxo-4,5 -dihydro-3H-pyrazolo [3 ,4- c] isoquinolin-9 -yl)ethyl)amino) -N -methoxypicolinamide ;

9-(l-((6-chloro-3'-fluoro-[2,4'-bipyridin]-3-yl)amino)-2- hydroxyethyl)-3-ethyl-4,7- dimethyl-3 ,4 -dihydro -5H-pyrazolo [3,4 -c] isoquinolin-5 -one ;

9-(l-((6-chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3-yl) amino)-2-hydroxyethyl)-3- ethyl-4,7-dimethyl-3,4-dihydro-5H-pyrazolo[3,4-c]isoquinolin -5-one;

9-( 1 -((6-chloro-2-( 1 ,5 -dimethyl- lH-pyrazol-4-yl)pyridin-3 -yl)amino)-2- hydroxyethyl)-3-ethyl-4,7-dimethyl-3,4-dihydro-5H-pyrazolo[3 ,4-c]isoquinolin-5-one;

9-( 1 -((6-chloro-2-( 1 -( 1 -methylpiperidin-4-yl)- lH-pyrazol-4-yl)pyridin-3- yl)amino)ethyl)-3 -ethyl -4, 7 -dimethyl-3 ,4-dihydro-5H-pyrazolo [3 ,4-c] isoquinolin-5 -one;

9-( 1 -((2-(4-acetylpiperazin- 1 -yl)-6-chloropyridin-3 -yl)amino)ethyl)-3 -ethyl -4, 7- dimethyl-3,4-dihydro-5H-pyrazolo[3,4-c]isoquinolin-5-one;

9-( 1 -((6-chloro-2-( 1 -methyl lH-pyrazol-4-yl)pyridin-3 -yl)amino)ethyl)-4,7-dimethyl- 3 -( 1 -methylazetidin-3 -yl) -3 ,4-dihydro-5H-pyrazolo [3 ,4-c] isoquinolin-5 -one ;

9-(l-((6-chloro-2-(l-methyl-lH-l,2,4-triazol-3-yl)pyridin -3-yl)amino)ethyl)-4,7- dimethyl-3 -( 1 -methylazetidin-3 -yl) -3 ,4-dihydro-5H-pyrazolo [3 ,4-c] isoquinolin-5 -one ;

9-( 1 -((6-chloro-2-( 1 -(2-hydroxyethyl)- 1H- 1 ,2,4-triazol-3 -yl)pyridin-3 - yl)amino)ethyl)-4,7-dimethyl-3-(l-methylazetidin-3-yl)-3,4-d ihydro-5H-pyrazolo[3,4- c]isoquinolin-5-one;

9-( 1 -((6-chloro-2-( lH-pyrazol-4-yl)pyridin-3-yl)amino)ethyl)-4,7 -dimethyl-3 -( 1 - methylazetidin-3-yl)-3,4-dihydro-5H-pyrazolo[3,4-c]isoquinol in-5-one;

9-(l-((6-chloro-r-methyl-6'-oxo-r,6'-dihydro-[2,3'-bipyri din]-3-yl)amino)ethyl)-4,7- dimethyl-3 -( 1 -methylazetidin-3 -yl) -3 ,4-dihydro-5H-pyrazolo [3 ,4-c] isoquinolin-5 -one ;

5 -(5 -chloro-2-(( 1 -(4,7-dimethyl-3 -( 1 -methylazetidin-3 -yl) -5 -oxo-4,5 -dihydro-3H- pyrazolo[3,4-c]isoquinolin-9-yl)ethyl)amino)phenyl)-N-methyl picolinamide;

9-(l-((6-chloro-2-(2-methyl-l-oxoisoindolin-5-yl)pyridin- 3-yl)amino)ethyl)-4,7- dimethyl-3 -( 1 -methylazetidin-3 -yl) -3 ,4-dihydro-5H-pyrazolo [3 ,4-c] isoquinolin-5 -one ;

9-( 1 -((6-chloro-2-( 1 -methyl- 1 H-indazol-5 -yl)pyridin-3 -yl)amino)ethyl)-4,7 -dimethyl - 3 -( 1 -methylazetidin-3 -yl) -3 ,4-dihydro-5H-pyrazolo [3 ,4-c] isoquinolin-5 -one ;

9-( 1 -((6-chloro-2-( 1 -methyl- 1 H- 1 ,2,4-triazol-3 -yl)pyridin-3 -yl)amino)ethyl)-3 -( 1 -(2- hydroxyethyl)azetidin-3 -yl)-4,7 -dimethyl-3 ,4-dihydro-5H-pyrazolo [3 ,4-c] isoquinolin-5 -one ;

9-( 1 -((6-chloro-2-( 1 -methyl- 1 H- 1 ,2,4-triazol-3 -yl)pyridin-3 -yl)amino)ethyl)-3 -( 1 - cyclopropylazetidin-3 -yl)-4,7-dimethyl-3 ,4-dihydro-5H-pyrazolo [3 ,4-c] isoquinolin-5 -one ; 9-( 1 -((6-chloro-2-( 1 -methyl- 1 H- 1 ,2,4-triazol-3 -yl)pyridin-3 -yl)amino)ethyl)-3 -( 1 - ((S)-2-hydroxypropyl)azetidin-3-yl)-4,7-dimethyl-3,4-dihydro -5H-pyrazolo[3,4- c]isoquinolin-5-one;

9-( 1 -((6-chloro-2-( 1 -methyl- 1 H- 1 ,2,4-triazol-3 -yl)pyridin-3 -yl)amino)ethyl)-3 -( 1 - ((R)-2-hydroxypropyl)azetidin-3-yl)-4,7-dimethyl-3,4-dihydro -5H-pyrazolo[3,4- c]isoquinolin-5-one;

9-( 1 -((6-chloro-2-( 1 -methyl- 1 H- 1 ,2,4-triazol-3 -yl)pyridin-3 -yl)amino)ethyl)-3 -( 1 -(2- hydroxy-2-methylpropyl)azetidin-3-yl)-4,7-dimethyl-3,4-dihyd ro-5H-pyrazolo[3,4- c]isoquinolin-5-one;

9-( 1 -((6-chloro-2-( 1 -methyl- 1 H- 1 ,2,4-triazol-3 -yl)pyridin-3 -yl)amino)ethyl)-3 -( 1 - (2,2-difluoroethyl)azetidin-3 -yl)-4,7-dimethyl-3 ,4-dihydro-5H-pyrazolo [3 ,4-c] isoquinolin-5 - one;

2-(3 -(9-( 1 -((6-chloro-2-( 1 -methyl-lH- 1 ,2,4-triazol-3 -yl)pyridin-3 -yl)amino)ethyl)- 4,7-dimethyl-5 -oxo-4,5 -dihydro-3H-pyrazolo [3,4-c] isoquinolin-3 -yl)azetidin- 1 - yl)acetonitrile;

3-(3 -(9-( 1 -((6-chloro-2-( 1 -methyl-lH- 1 ,2,4-triazol-3 -yl)pyridin-3 -yl)amino)ethyl)- 4,7-dimethyl-5 -oxo-4,5 -dihydro-3H-pyrazolo [3,4-c] isoquinolin-3 -yl)azetidin- 1 - yl)propanenitrile;

3 -( 1 -acetylazetidin-3 -yl)-9-( 1 -((6-chloro-2-( 1 -methyl- 1 H- 1 ,2,4-triazol-3 -yl)pyridin-3 - yl)amino)ethyl)-4,7-dimethyl-3,4-dihydro-5H-pyrazolo[3,4-c]i soquinolin-5-one; methyl 3 -(9-( 1 -((6-chloro-2-( 1 -methyl-lH-1 ,2,4-triazol-3 -yl)pyridin-3 - yl)amino)ethyl)-4,7-dimethyl-5-oxo-4,5-dihydro-3H-pyrazolo[3 ,4-c]isoquinolin-3- yl)azetidine - 1 -carboxylate ;

9-(l-((6-chloro-2-(l-methyl-lH-l,2,4-triazol-3-yl)pyridin -3-yl)amino)ethyl)-4,7- dimethyl-3-(l-(methylsulfonyl)azetidin-3-yl)-3,4-dihydro-5H- pyrazolo[3,4-c]isoquinolin-5- one;

3-(l-acetylazetidin-3-yl)-9-(l-((6-chloro-r-methyl-6'-oxo -r,6'-dihydro-[2,3'- bipyridin] -3 -yl)amino)ethyl)-4,7-dimethyl-3 ,4-dihydro-5H-pyrazolo [3 ,4-c] isoquinolin-5 -one ;

3-((l-(3-(l-acetylazetidin-3-yl)-4,7-dimethyl-5-oxo-4,5-d ihydro-3H-pyrazolo[3,4- c]isoquinolin-9-yl)ethyl)amino)-6-chloro-N-methyl-[2,3'-bipy ridine]-6'-carboxamide;

3-(l-acetylazetidin-3-yl)-9-(l-((6-chloro-2-(l-(2-hydroxy ethyl)-lH-l,2,4-triazol-3- yl)pyridin-3 -yl)amino)ethyl)-4,7-dimethyl-3 ,4-dihydro-5H-pyrazolo [3 ,4-c] isoquinolin-5 -one ;

9-( 1 -((6-chloro-2-( 1 -methyl- 1 H- 1 ,2,4-triazol-3 -yl)pyridin-3 -yl)amino)ethyl)-3 -( 1 -(2- hydroxyacetyl)azetidin-3-yl)-4,7-dimethyl-3,4-dihydro-5H-pyr azolo[3,4-c]isoquinolin-5-one; 9-( 1 -((6-chloro-2-( 1 -methyl- 1 H- 1 ,2,4-triazol-3 -yl)pyridin-3 -yl)amino)ethyl)-3 -( 1 - (dimethylglycyl)azetidin-3-yl)-4,7-dimethyl-3,4-dihydro-5H-p yrazolo[3,4-c]isoquinolin-5- one;

9-(l-((6-chloro-2-(l-methyl-lH-l,2,4-triazol-3-yl)pyridin -3-yl)amino)ethyl)-7- methyl -4-(methyl-d3)-3-((S)-l-methylpyrrolidin-3-yl)-3,4-dihydro-5 H-pyrazolo[3, 4- c]isoquinolin-5-one;

9-(l-((6-chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3-yl) amino)ethyl)-3-((l-(2,2- difluoroethyl)piperidin-4-yl)methyl)-4,7-dimethyl-3,4-dihydr o-5H-pyrazolo[3,4- c]isoquinolin-5-one;

3-(( 1 -acetylpiperidin-4-yl)methyl)-9-( 1 -((6-chloro-2-( 1 -methyl- lH-pyrazol-4- yl)pyridin-3 -yl)amino)ethyl)-4,7-dimethyl-3 ,4-dihydro-5H-pyrazolo [3 ,4-c] isoquinolin-5 -one ;

4-((9-(l-((6-chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3 -yl)amino)ethyl)-4,7- dimethyl-5 -oxo -4,5 -dihydro -3H-pyrazolo [3,4 -c] isoquinolin-3 -yl)methyl) -N,N - dimethylpiperidine - 1 -carboxamide ;

4-((9-(l-((6-chloro-3'-fluoro-[2,4'-bipyridin]-3-yl)amino )ethyl)-4,7-dimethyl-5-oxo-

4,5 -dihydro -3H-pyrazolo [3,4 -c] isoquinolin-3 -yl)methyl) -N -ethylpiperidine - 1 -carboxamide ;

9-( 1 -((6-chloro-3 '-fluoro-[2,4'-bipyridin] -3 -yl)amino)ethyl)-3 -(( 1 -(2,2- difluoroethyl)pyrrolidin-3-yl)methyl)-4,7-dimethyl-3,4-dihyd ro-5H-pyrazolo[3,4- c]isoquinolin-5-one;

9-( 1 -((6-chloro-2-( 1 -methyl- 1 H- 1 ,2,4-triazol-3 -yl)pyridin-3 -yl)amino)ethyl)-3 -( 1 - hydroxy ethyl) -4,7 -dimethylimidazo [ 1 , 5 -a] quinazolin-5 (4H) -one ;

9-(l-((6-chloro-2-(l-methyl-lH-l,2,4-triazol-3-yl)pyridin -3-yl)amino)ethyl)-4,7- dimethyl-3-(pyridin-3-yl)imidazo[l,5-a]quinazolin-5(4H)-one;

9-(l-((6-chloro-2-(l-methyl-lH-l,2,4-triazol-3-yl)pyridin -3-yl)amino)ethyl)-4,7- dimethyl-3-(2-methylpyridin-3-yl)imidazo[l,5-a]quinazolin-5( 4H)-one;

9-(l-((6-chloro-2-(l-methyl-lH-l,2,4-triazol-3-yl)pyridin -3-yl)amino)ethyl)-4,7- dimethyl-3 -(pyrimidin-5 -yl)imidazo [1,5 -a] quinazolin-5 (4H)-one ;

9-(l-((6-chloro-2-(l-methyl-lH-l,2,4-triazol-3-yl)pyridin -3-yl)amino)ethyl)-4,7- dimethyl-3-(2-methylpyridin-4-yl)imidazo[l,5-a]quinazolin-5( 4H)-one;

9-(l-((6-chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3-yl) amino)ethyl)-4,7-dimethyl- 3 -(2 -methylpyridin-3 -yl)imidazo [ 1 ,5 -a] quinazolin-5 (4H)-one ;

9-(l-((6-chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3-yl) amino)ethyl)-4,7-dimethyl- 3 -(pyrimidin-5 -yl)imidazo [ 1 ,5 -a] quinazolin-5 (4H)-one ;

4-(9-(l-((6-chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3- yl)amino)ethyl)-4,7- dimethyl-5 -oxo -4,5 -dihydroimidazo [ 1 , 5 -a] quinazolin-3 -yl) -N -methylpicolinamide ; 9-(l-((6-chloro-2-(l-methyl-lH-l,2,4-triazol-3-yl)pyridin-3- yl)amino)ethyl)-N,N,4,7- tetramethyl-5 -oxo-4,5 -dihydroimidazo [ 1 ,5 -a] quinazoline-3 -carboxamide ;

9-(l-((6-chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3-yl) amino)ethyl)-N,N,4,7- tetramethyl-5 -oxo-4,5 -dihydroimidazo [ 1 ,5 -a] quinazoline-3 -carboxamide ;

3-(azetidine-l-carbonyl)-9-(l-((6-chloro-2-(l-methyl-lH-p yrazol-4-yl)pyridin-3- yl)amino)ethyl) -4,7 -dimethylimidazo [ 1 , 5 -a] quinazolin-5 (4H) -one ;

9-(l-((6-chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3-yl) amino)ethyl)-3-(3- hydroxyazetidine - 1 -carbonyl) -4,7 -dimethylimidazo [ 1 ,5 -a] quinazolin-5 (4H)-one ;

9-( 1 -((6-chloro-2-( 1 -methyl- 1 H- 1 ,2,4-triazol-3 -yl)pyridin-3 -yl)amino)ethyl)-4-(2,2- difluoroethyl) -N,N, 7 -trimethyl -5 -oxo -4,5 -dihydroimidazo [ 1 , 5 -a] quinazoline-3 -carboxamide ;

9-(l-((6-chloro-2-(3-methyl-lH-pyrazol-4-yl)pyridin-3-yl) amino)ethyl)-N,N,4,7- tetramethyl-5 -oxo-4,5 -dihydroimidazo [ 1 ,5 -a] quinazoline-3 -carboxamide ;

9-(l-((6-chloro-2-(4-cyano-2-fluorophenyl)pyridin-3-yl)am ino)ethyl)-N,N,4,7- tetramethyl-5 -oxo-4,5 -dihydroimidazo [ 1 ,5 -a] quinazoline-3 -carboxamide ;

9-(l-((6-chloro-2-(4-cyanophenyl)pyridin-3-yl)amino)ethyl )-N,N,4,7-tetramethyl-5- oxo-4,5 -dihydroimidazo [ 1 ,5 -a] quinazoline-3 -carboxamide;

9-(l-((6-chloro-2-morpholinopyridin-3-yl)amino)ethyl)-N,N ,4,7-tetramethyl-5-oxo- 4,5 -dihydroimidazo [ 1 ,5 -a] quinazoline -3 -carboxamide ;

9-(l-((4-fluoro-2-(l-methyl-lH-pyrazol-4-yl)phenyl)amino) ethyl)-N,N,4,7- tetramethyl-5 -oxo-4,5 -dihydroimidazo [ 1 ,5 -a] quinazoline-3 -carboxamide ;

9-( 1 -((6-chloro-2-( 1 ,5-dimethyl- lH-pyrazol-4-yl)pyridin-3 -yl)amino)ethyl)-N,N,4,7- tetramethyl-5 -oxo-4,5 -dihydroimidazo [ 1 ,5 -a] quinazoline-3 -carboxamide ;

9-( 1 -((6-chloro-2-( 1 ,3-dimethyl- lH-pyrazol-4-yl)pyridin-3 -yl)amino)ethyl)-N,N,4,7- tetramethyl-5 -oxo-4,5 -dihydroimidazo [ 1 ,5 -a] quinazoline-3 -carboxamide ;

9-(l-((6-chloro-2-(l-methyl-lH-l,2,4-triazol-3-yl)pyridin -3-yl)amino)ethyl)-4,7- dimethyl-3 -( 1 -methylpiperidin-4-yl)imidazo [1,5 -a] quinazolin-5 (427) -one ;

3 -( 1 -acetylpiperidin-4-yl)-9-( 1 -((6-chloro-2-( 1 -methyl- 1H- 1 ,2,4-triazol-3 -yl)pyridin- 3-yl)amino)ethyl)-4,7-dimethylimidazo[l,5-a]quinazolin-5(4H) -one; ethyl 4-(9-(l-((6-chloro-2-(l-methyl-lH-l,2,4-triazol-3-yl)pyridin -3-yl)amino)ethyl)- 4,7-dimethyl-5 -oxo-4,5 -dihydroimidazo [1,5 -a]quinazolin-3 -yl)piperidine- 1 -carboxylate;

9-(l-((6-chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3-yl) amino)ethyl)-4,7-dimethyl- 3 -( 1 -methylpiperidin-4-yl)imidazo [ 1 ,5 -a] quinazolin-5 (4H)-one ;

9-( 1 -((6-chloro-2-( 1 -methyl- 17/-pyrazol-4-yl)pyridin-3-yl)amino)cthyl)-4.7-dimcthyl- 3 -( 1 -methylpyrrolidin-3 -yl) -3 ,4-dihydro-5H-pyrazolo [3 ,4-c]isoquinolin-5 -one; 9-(l-((6-chloro-2-(l-methyl-lH-l,2,4-triazol-3-yl)pyridin-3- yl)amino)ethyl)-4,7- dimethyl-3 -( 1 -methylpyrrolidin-3 -yl) -3 ,4-dihydro-5H-pyrazolo [3 ,4-c] isoquinolin-5 -one;

9-( 1 -((6-chloro-2-( 1 -methyl- 1 H- 1 ,2,4-triazol-3 -yl)pyridin-3 -yl)amino)ethyl)-3 -( 1 - ethylpyrrolidin-3 -yl)-4,7-dimethyl-3 ,4-dihydro-5H-pyrazolo [3 ,4-c] isoquinolin-5 -one ;

9-( 1 -((6-chloro-2-( 1 -methyl- 1 H- 1 ,2,4-triazol-3 -yl)pyridin-3 -yl)amino)ethyl)-3 -( 1 -(2- hydroxyethyl)pyrrolidin-3-yl)-4,7-dimethyl-3,4-dihydro-5H-py razolo[3,4-c]isoquinolin-5- one;

9-(l-((6-chloro-2-(2-methyl-2H-tetrazol-5-yl)pyridin-3-yl )amino)ethyl)-7-methyl-4- (methyl -d3)-3-(l-methylpyrrolidin-3-yl)-3,4-dihydro-5H-pyrazolo[3,4 -c]isoquinolin-5-one;

9-(l-((6-chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3-yl) amino)ethyl)-3-(l-(2- hydroxyethyl)pyrrolidin-3-yl)-7 -methyl -4 -(methyl -d3)-3,4-dihydro-5H-pyrazolo[3, 4- c]isoquinolin-5-one;

9-( 1 -((6-chloro-3 '-fluoro-[2,4'-bipyridin] -3 -yl)amino)ethyl)-3 -( 1 -isopropylpyrrolidin- 3-yl)-4,7-dimethyl-3,4-dihydro-5H-pyrazolo[3,4-c]isoquinolin -5-one;

9-(l-((6-chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3-yl) amino)ethyl)-3-(l-(2,2- difluoroethyl)pyrrolidin-3-yl)-4,7-dimethyl-3,4-dihydro-5H-p yrazolo[3,4-c]isoquinolin-5- one;

9-(l-((6-chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3-yl) amino)ethyl)-3-(l-(2,2- difluoroethyl)pyrrolidin-3-yl)-7 -methyl -4-(methyl-d3)-3,4-dihydro-5H-pyrazolo[3, 4- c]isoquinolin-5-one;

2-(3 -(9-( 1 -((6-chloro-2-( 1 -methyl - 1 H-pyrazol -4 -yl)pyridin-3 -yl)amino)ethyl)-7 - methyl -4-(mcthyl-tA)-5-oxo-4.5-dihydro-3//-pyrazolo|3.4-c|isoqiiin olin-3-yl)pyrrolidin- 1 - yl)acetonitrile;

9-( 1 -((6-chloro-2-( 1 -methyl- 1H- 1 ,2,4-triazol-3 -yl)pyridin-3 -yl)amino)ethyl)-4-ethyl- 3-( 1 -(2-hydroxyethyl)pyrrolidin-3-yl)-7 -methyl -3, 4-dihydro-5H-pyrazolo[3,4-c]isoquinolin- 5 -one;

9-( 1 -((6-chloro-2-( 1 -methyl- 1 H- 1 ,2,4-triazol-3 -yl)pyridin-3 -yl)amino)ethyl)-4-ethyl- 3-(l-(2-hydroxy-2-methylpropyl)pyrrolidin-3-yl)-7-methyl-3,4 -dihydro-5H-pyrazolo[3,4- c]isoquinolin-5 -one ;

9-( 1 -((6-chloro-2-( 1 -methyl- 1 H- 1 ,2,4-triazol-3 -yl)pyridin-3 -yl)amino)ethyl)-4-ethyl- 3-(l-isopropylpyrrolidin-3-yl)-7-methyl-3,4-dihydro-5H-pyraz olo[3,4-c]isoquinolin-5-one;

9-( 1 -((6-chloro-2-( 1 -methyl- 1 H- 1 ,2,4-triazol-3 -yl)pyridin-3 -yl)amino)ethyl)-4-ethyl- 7 -methyl-3 -( 1 -(methylsulfonyl)pyrrolidin-3 -y 1) -3 ,4-dihydro-5H-pyrazolo [3 ,4-c] isoquinolin-5 - one; 3 -( 1 -acetylpyrrolidin-3 -yl)-9-( 1 -((6-chloro-2-( 1 -methyl- \H- 1 ,2,4-triazol-3 -yl)pyridin- 3 -yl)amino)ethyl)-4-ethyl-7-methyl-3 ,4-dihydro-5H-pyrazolo [3 ,4-c] isoquinolin-5 -one ; methyl 3 -(9-( 1 -((6-chloro-2-( 1 -methyl - 1 //- 1 ,2,4-triazol-3 -yl)pyridin-3 - yl)amino)ethyl)-4-ethyl-7-methyl-5-oxo-4,5-dihydro-3H-pyrazo lo[3,4-c]isoquinolin-3- yl)pyrrolidine- 1 -carboxylate ;

3-(9-(l-((6-chloro-3'-fluoro-[2,4'-bipyridin]-3-yl)amino) ethyl)-4,7-dimethyl-5-oxo-

4,5 -dihydro -3H-pyrazolo [3,4 -c] isoquinolin-3 -yl)-N,N -dimethylpyrrolidine - 1 -carboxamide ;

3-(3-(9-(l-((6-chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin -3-yl)amino)ethyl)-7- methyl-4-(methyl-d3)-5-oxo-4,5-dihydro-3H-pyrazolo[3,4-c]iso quinolin-3-yl)pyrrolidin-l- yl)propanenitrile;

3-(3 -(9-( 1 -((6-chloro-2-(2-methyl-2H-tetrazol-5 -yl)pyridin-3 -yl)amino)ethyl)-7 - methyl -4-(methyl-d3)-5-oxo-4, 5-dihydro-3H-pyrazolo[3, 4-c] isoquinolin-3 -yl)pyrrolidin-l - yl)propanenitrile;

9-( 1 -((6-chloro-2-( 1 -methyl- lH-pyrazol-4-yl)pyridin-3-yl)amino)ethyl)-3-( 1 -(2- hydroxyacctyl)pyrrolidm-3-yl)-7-mcthyl-4-(mcthyl-tA)-3.4-dih ydro-5//-pyrazolo|3.4- c]isoquinolin-5-one;

9-(l-((6-chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3-yl) amino)ethyl)-3-(l- (dimcthylglycyl)pyrrolidin-3-yl)-7-mcthyl-4-(mcthyl-J’,)-3 .4-dihydro-5//-pyrazolo|3.4- c]isoquinolin-5-one;

9-(l-((6-chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3-yl) amino)ethyl)-7-methyl-4- (methyl -d3)-3 -( 1 -( 1 -methyl- 1 H-pyrazole-5 -carbonyl)pyrrolidin-3 -yl)-3 ,4-dihydro-5H- pyrazolo [3 ,4-c]isoquinolin-5 -one ;

1 -(3 -(9-( 1 -((6-chloro-2-( 1 -methyl -IH-pyrazol -4 -yl)pyridin-3 -yl)amino)ethyl)-7 - methyl-4-(methyl-d3)-5-oxo-4,5-dihydro-3H-pyrazolo[3,4-c]iso quinolin-3-yl)pyrrolidine-l- carbonyl)cyclopropane- 1 -carbonitrile;

9-(l-((6-chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3-yl) amino)ethyl)-4,7-dimethyl- 3-(l-methylpiperidin-4-yl)-3,4-dihydro-5H-pyrazolo[3,4-c]iso quinolin-5-one;

9-[l-[[6-chloro-2-(l-methyl-l,2,4-triazol-3-yl)-3-pyridyl ]amino]ethyl]-4,7-dimethyl- 3-(l-methyl-4-piperidyl)pyrazolo[3,4-c]isoquinolin-5-one;

9-[ 1 -[[6-chloro-2-( 1 -ethylpyrazol-4-yl)-3-pyridyl]amino]ethyl] -4,7 -dimethyl -3 -( 1 - methyl -4 -piperidyl)pyrazolo [3 ,4-c] isoquinolin-5 -one ;

9-[l-[[6-chloro-2-(2-methyltetrazol-5-yl)-3-pyridyl]amino ]ethyl]-4,7-dimethyl-3-(l- methyl -4 -piperidyl)pyrazolo [3 ,4-c] isoquinolin-5 -one ;

9-[ 1 -[[6-chloro-2-( 1 ,3,4-oxadiazol-2-yl)-3-pyridyl]amino]ethyl] -4,7-dimethyl-3 -( 1 - methyl -4 -piperidyl)pyrazolo [3 ,4-c] isoquinolin-5 -one ; 5-[6-chloro-3-[l-[4,7-dimethyl-3-(l-methyl-4-piperidyl)-5-ox o-pyrazolo[3,4- c] isoquinolin -9 -yl] ethylamino] -2 -pyridyl] -JV-methyl -pyridine -2 -carboxamide ;

3-[6-chloro-3-[l-[4,7-dimethyl-3-(l-methyl-4-piperidyl)-5 -oxo-pyrazolo[3,4- c]isoquinolin-9-yl]ethylamino]-2-pyridyl]-4H-l,2,4-oxadiazol -5-one;

9-[ 1 -[[6-chloro-2-( 1 , 3 -dimethylpyrazol -4 -yl) -3 -pyridyl] amino] ethyl] -4,7 -dimethyl -3 - (l-methyl-4-piperidyl)pyrazolo[3,4-c]isoquinolin-5-one;

9-[ 1 -[[6-chloro-2-( 1 -methyl -6 -oxo-3-pyridyl)-3-pyridyl]amino]ethyl] -4,7 -dimethyl -3 - (l-methyl-4-piperidyl)pyrazolo[3,4-c]isoquinolin-5-one;

9-[l-[[6-chloro-2-(4-hydroxy-l-piperidyl)-3-pyridyl]amino ]ethyl]-4,7-dimethyl-3-(l- methyl -4 -piperidyl)pyrazolo [3 ,4-c] isoquinolin-5 -one ;

9-[ 1 -[[6-chloro-2-(3 -fluoro-4-pyridyl)-3 -pyridyl] amino] ethyl] -4,7-dimethyl-3-( 1 - methyl -4-piperidyl)pyrazolo [3 ,4-c] isoquinolin-5 -one;

9-[ 1 -[[6-chloro-2-(3 -pyridyl)-3 -pyridyl] amino] ethyl] -4,7-dimethyl-3-( 1 -methyl -4- piperidyl)pyrazolo [3 ,4-c] isoquinolin-5 -one;

5-[3-[ l-[4,7-dimethyl-3-(l -methyl -4-piperidyl)-5-oxo-pyrazolo[3,4-c]isoquinolin-9- yl] ethylamino] -6-methyl -2 -pyridyl] -N -methyl -pyridine-2 -carboxamide ;

9-[ 1 -[[6-chloro-2-( 1 -methyl -2 -oxo-4-pyridyl)-3-pyridyl]amino]ethyl] -4,7 -dimethyl -3 - (l-methyl-4-piperidyl)pyrazolo[3,4-c]isoquinolin-5-one;

9-[ 1 -[[6-chloro-2-( 1 -methylpyrazol-4-yl)-3 -pyridyl] amino] ethyl] -3 -[ 1 -(2-hydroxy-2- methyl-propyl)-4-piperidyl]-4,7-dimethyl-pyrazolo[3,4-c]isoq uinolin-5-one;

9-[ 1 -[[6-chloro-2-( 1 -methylpyrazol-4-yl)-3 -pyridyl] amino] ethyl] -3 -[ I -| (2/?)-2- hydroxypropyl] -4-piperidyl] -4,7 -dimethyl -pyrazolo [3 ,4-c] isoquinolin-5 -one ;

9-[ 1 -[ [6-chloro-2-( 1 -methylpyrazol-4-yl)-3 -pyridyl] amino] ethyl] -3 -[ I -| (2.S')-2- hydroxypropyl] -4-piperidyl] -4,7 -dimethyl -pyrazolo [3 ,4-c] isoquinolin-5 -one ;

9-( 1 -((6-chloro-2-( 1 -methyl- 1 H- 1 ,2,4-triazol-3 -yl)pyridin-3 -yl)amino)ethyl)-3 -( 1 -(2- hydroxy-2-methylpropyl)piperidin-4-yl)-4,7-dimethyl-3,4-dihy dro-5H-pyrazolo[3,4- c]isoquinolin-5-one;

9-(l-((6-chloro-2-(l-methyl-lH-l,2,4-triazol-3-yl)pyridin -3-yl)amino)ethyl)-4,7- dimethyl-3-(piperidin-4-yl)-3,4-dihydro-5H-pyrazolo[3,4-c]is oquinolin-5-one;

9-[l-[[6-chloro-2-(l-methyl-l,2,4-triazol-3-yl)-3-pyridyl ]amino]ethyl]-3-[l-(2- hydroxyethyl)-4-piperidyl]-4,7-dimethyl-pyrazolo[3,4-c]isoqu inolin-5-one;

9-(l-((6-chloro-2-(l-methyl-lH-l,2,4-triazol-3-yl)pyridin -3-yl)amino)ethyl)-4,7- dimethyl-3-(l-((l-methyl-lH-imidazol-2-yl)methyl)piperidin-4 -yl)-3,4-dihydro-5H- pyrazolo [3 ,4-c] isoquinolin-5 -one ; 9-(l-((6-chloro-2-(l-methyl-lH-l,2,4-triazol-3-yl)pyridin-3- yl)amino)ethyl)-4,7- dimethyl-3 -( 1 -(oxetan-3 -yl)piperidin-4-yl)-3 ,4-dihydro-5H-pyrazolo [3 ,4-c] isoquinolin-5 -one ;

3-[4-[9-[l-[[6-chloro-2-(l-methyl-l,2,4-triazol-3-yl)-3-p yridyl]amino]ethyl]-4,7- dimethyl-5 -oxo -pyrazolo [3,4 -c] isoquinolin-3 -y 1] - 1 -piperidyl] propanenitrile ;

9-( 1 -((6-chloro-2-( 1 -methyl- 1 H- 1 ,2,4-triazol-3 -yl)pyridin-3 -yl)amino)ethyl)-3 -( 1 - (2,2-difluoroethyl)piperidin-4-yl)-4,7-dimethyl-3,4-dihydro- 5H-pyrazolo[3,4-c]isoquinolin-5- one;

3 -( 1 -acetylpiperidin-4-yl)-9-( 1 -((6-chloro-2-( 1 -methyl- lH-pyrazol-4-yl)pyridin-3 - yl)amino)ethyl)-4,7-dimethyl-3,4-dihydro-5H-pyrazolo[3,4-c]i soquinolin-5-one;

9-[ 1 -[ [6-chloro-2-( 1 -methylpyrazol-4-yl)-3 -pyridyl] amino] ethyl] -4,7-dimethyl-3 -( 1 - methylsulfonyl -4-piperidyl)pyrazolo [3 ,4-c] isoquinolin-5 -one; methyl 4-[9-[l-[[6-chloro-2-(l-methylpyrazol-4-yl)-3-pyridyl]amino] ethyl]-4,7- dimethyl-5 -oxo -pyrazolo [3,4 -c] isoquinolin-3 -yl]piperidine - 1 -carboxylate ;

9-[ 1 -[ [6-chloro-2-( 1 -methylpyrazol-4-yl)-3 -pyridyl] amino] ethyl] -3 -( 1 - cyclopropylsulfonyl -4-piperidyl)-4,7-dimethyl-pyrazolo[3,4-c]isoquinolin-5-one;

4-(9-(l-((6-chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3- yl)amino)ethyl)-4,7- dimethyl-5-oxo-4,5-dihydro-3H-pyrazolo[3,4-c]isoquinolin-3-y l)-N,N-dimethylpiperidine-l- carboxamide;

3 -( 1 -acetylpiperidin-4-yl)-9-( 1 -((6-chloro- 1 '-methyl-6'-oxo- 1 ',6'-dihydro-[2,3 '- bipyridin] -3 -yl)amino)ethyl)-4,7-dimethyl-3 ,4-dihydro-5H-pyrazolo [3 ,4-c] isoquinolin-5 -one ;

3 -( 1 -acetylpiperidin-4-yl)-9-( 1 -((6-chloro-2-( 1 -methyl- 1H- 1 ,2,4-triazol-3 -yl)pyridin- 3-yl)amino)ethyl)-4,7-dimethyl-3,4-dihydro-5H-pyrazolo[3,4-c ]isoquinolin-5-one;

4,7-dimethyl-9-(l-((6-methyl-2-(l-methyl-lH-pyrazol-4-yl) pyridin-3- yl)amino)ethyl)-3-(l-(methylsulfonyl)piperidin-4-yl)-3,4-dih ydro-5H-pyrazolo[3,4- c]isoquinolin-5-one;

9-(l-((6-chloro-2-(l-methyl-lH-l,2,4-triazol-3-yl)pyridin -3-yl)amino)ethyl)-4,7- dimethyl-3 -( 1 -(methylsulfonyl)piperidin-4-yl)-3 ,4-dihydro-5H-pyrazolo [3 ,4-c] isoquinolin-5 - one;

9-(l-((6-chloro-2-(2-methyl-l-oxoisoindolin-5-yl)pyridin- 3-yl)amino)ethyl)-4,7- dimethyl-3 -( 1 -(methylsulfonyl)piperidin-4-yl)-3 ,4-dihydro-5H-pyrazolo [3 ,4-c] isoquinolin-5 - one;

9-(l-((6-chloro-2-(quinolin-6-yl)pyridin-3-yl)amino)ethyl )-4,7-dimethyl-3-(l- (methylsulfonyl)piperidin-4-yl)-3 ,4-dihydro-5H-pyrazolo [3 ,4-c] isoquinolin-5 -one ;

9-[ 1 -[[6-chloro-2-( 1 -methylpyrazol-4-yl)-3 -pyridyl] amino] ethyl] -3 -[ 1 -(2- hydroxyacetyl)-4-piperidyl]-4,7-dimethyl-pyrazolo[3,4-c]isoq uinolin-5-one; 1 -(4-(9-( 1 -((6-chloro-2-( 1 -methyl-lH- 1 ,2,4-triazol-3 -yl)pyridin-3 -yl)amino)ethyl)- 4,7-dimethyl-5-oxo-4,5-dihydro-3H-pyrazolo[3,4-c]isoquinolin -3-yl)piperidine-l- carbonyl)cyclopropane- 1 -carbonitrile;

9-(l-((6-chloro-2-(l-methyl-lH-l,2,4-triazol-3-yl)pyridin -3-yl)amino)ethyl)-4,7- dimethyl-3-( 1 -( 1 -methyl- lH-pyrazole-5 -carbonyl)piperidin-4-yl)-3,4-dihydro-5H- pyrazolo [3 ,4-c]isoquinolin-5 -one ;

9-(l-((6-chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3-yl) amino)ethyl)-3-(l- (dimethylglycyl)piperidin-4-yl)-4,7-dimethyl-3,4-dihydro-5H- pyrazolo[3,4-c]isoquinolin-5- one;

9-(l-((6-chloro-2-(l-methyl-lH-l,2,4-triazol-3-yl)pyridin -3-yl)amino)ethyl)-4,7- dimethyl-3 -( 1 -(methyl -L-prolyl)piperidin -4-yl)-3 ,4-dihydro-5H-pyrazolo [3 ,4-c] isoquinolin-5 - one;

9-(l-((6-chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3-yl) amino)ethyl)-4-methyl-3- (l-methylpiperidin-4-yl)-5-oxo-4,5-dihydro-3H-pyrazolo[3,4-c ]isoquinoline-7 -carbonitrile;

9-( 1 -((6-chloro-2-( 1 -methyl- 1H- 1 ,2,4-triazol-3 -yl)pyridin-3 -yl)amino)ethyl)-4-ethyl- 7 -methyl -2-(tetrahydro-2H-pyran-4-yl)-2,4-dihydro-5H-pyrazolo[3,4-c] isoquinolin-5-one;

3-(6-chloro-3-((l-(4,7-dimethyl-5-oxo-2-(tetrahydro-2H-py ran-4-yl)-4,5-dihydro-2H- pyrazolo[3,4-c]isoquinolin-9-yl)ethyl)amino)pyridin-2-yl)-l, 2,4-oxadiazol-5(4H)-one;

9-( 1 -((6-chloro-2-( 1 -methyl- 1H- 1 ,2,4-triazol-3 -yl)pyridin-3 -yl)amino)ethyl)-4-ethyl- 7-methyl-2-(l-methylpiperidin-4-yl)-2,4-dihydro-5H-pyrazolo[ 3,4-c]isoquinolin-5-one;

2-( 1 -acetylpiperidin-4-yl)-9-( 1 -((6-chloro-2-( 1 -methyl- 1H- 1 ,2,4-triazol-3 -yl)pyridin- 3 -yl)amino)ethyl)-4 -ethyl-7-methyl -2, 4-dihydro-5H-pyrazolo [3 ,4-c] isoquinolin-5 -one ;

9-( 1 -((6-chloro-2-( 1 -methyl- 1H- 1 ,2,4-triazol-3 -yl)pyridin-3 -yl)amino)ethyl)-4-ethyl- 2-(l -(2-hydroxyethyl)piperidin-4-yl)-7 -methyl -2, 4-dihydro-5H-pyrazolo[3,4-c]isoquinolin-5- one;

3-(4-(9-( 1 -((6-chloro-2-( 1 -methyl - 1 H - 1 ,2,4-triazol-3 -yl)pyridin-3 -yl)amino)ethyl)-4- ethyl-7-methyl-5-oxo-4,5-dihydro-2H-pyrazolo[3,4-c]isoquinol in-2-yl)piperidin-l- yl)propanenitrile;

9-( 1 -((6-chloro-2-( 1 -methyl- 1H- 1 ,2,4-triazol-3 -yl)pyridin-3 -yl)amino)ethyl)-4-ethyl- 7 -methyl -2-( 1 -methylazetidin-3-yl)-2,4-dihydro-5H-pyrazolo[3,4-c]isoquino lin-5-one;

9-(l-((6-chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3-yl) amino)ethyl)-4,7-dimethyl- 2-(l-methylpiperidin-4-yl)-2,4-dihydro-5H-pyrazolo[3,4-c]iso quinolin-5-one;

9-(l-((6-chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3-yl) amino)ethyl)-2-(l- isopropylpiperidin-4-yl)-4,7-dimethyl-2,4-dihydro-5H-pyrazol o [3 ,4-c] isoquinolin-5 -one ; 9-(l-((6-chloro-2-(l-methyl-lH-l,2,4-triazol-3-yl)pyridin-3- yl)amino)ethyl)-4,7- dimethyl-2-(l-methylpiperidin-4-yl)-2,4-dihydro-5H-pyrazolo[ 3,4-c]isoquinolin-5-one;

2-( 1 -acetylpiperidin-4-yl)-9-( 1 -((6-chloro-2-( 1 -methyl- lH-pyrazol-4-yl)pyridin-3 - yl)amino)ethyl)-4,7-dimethyl-2,4-dihydro-5H-pyrazolo[3,4-c]i soquinolin-5-one; ethyl 4-(9-(l-((6-chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3-yl) amino)ethyl)-4,7- dimethyl-5 -oxo -4,5 -dihydro -2H-pyrazolo [3,4 -c] isoquinolin-2 -yl)piperidine- 1 -carboxylate ;

4-(9-(l-((6-chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3- yl)amino)ethyl)-4,7- dimethyl-5 -oxo -4,5 -dihydro -2H-pyrazolo [3,4 -c] isoquinolin-2 -yl) -N,N -dimethylpiperidine- 1 - carboxamide;

9-(l-((6-chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3-yl) amino)ethyl)-2-(l-(2,2- difluoroethyl)piperidin-4-yl)-4,7-dimethyl-2,4-dihydro-5H-py razolo[3,4-c]isoquinolin-5-one;

3-[6-chloro-3-[l-[7-methyl-5-oxo-3-[(3S)-tetrahydrofuran- 3-yl]-4- (trideuteriomethyl)pyrazolo [3 ,4-c] isoquinolin-9-yl]ethylamino] -2 -pyridyl] -4H- 1 ,2,4- oxadiazol-5-one;

9-(l-((6-chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3-yl) amino)ethyl)-7-methyl-4- (methyl -d3)-3-((S)-tetrahydrofuran-3-yl)-3,4-dihydro-5H-pyrazolo[3, 4-c]isoquinolin-5-one;

3-(6-chloro-3-((l-(4-ethyl-7-methyl-5-oxo-3-((S)-tetrahyd rofuran-3-yl)-4,5-dihydro- 3H-pyrazolo[3,4-c]isoquinolin-9-yl)ethyl)amino)pyridin-2-yl) -l,2,4-oxadiazol-5(4H)-one;

9-( 1 -((6-chloro-2-( 1 -methyl- 1 H- 1 ,2,4-triazol-3 -yl)pyridin-3 -yl)amino)ethyl)-4-ethyl- 7-methyl-3-((S)-tetrahydrofuran-3-yl)-3,4-dihydro-5H-pyrazol o[3,4-c]isoquinolin-5-one;

9-( 1 -((6-chloro-2-( 1 -( 1 -methylpiperidin-4-yl)-lH-pyrazol-4-yl)pyridin-3- yl)amino)ethyl)-4-ethyl-7-methyl-3-((S)-tetrahydrofuran-3-yl )-3,4-dihydro-5H-pyrazolo[3,4- c] isoquinolin-5 -one ;

9-(l-((6-chloro-2'-methyl-[2,4'-bipyridin]-3-yl)amino)eth yl)-4-ethyl-7-methyl-3-((S)- tetrahydrofuran-3-yl)-3,4-dihydro-5H-pyrazolo[3,4-c]isoquino lin-5-one;

9-(l-((6-chloro-r-methyl-6'-oxo-r,6'-dihydro-[2,3'-bipyri din]-3-yl)amino)ethyl)-4- ethyl-7 -methyl -3 -( (.S') -tetrahydro furan-3 -y 1) -3 ,4-dihydro-5H-pyrazolo [3 ,4-c] isoquinolin-5 - one;

6-chloro-3 -(( 1 -(4-ethyl-7-methyl-5 -oxo-3 -((.S')-tctrahydrofuran-3 -yl)-4,5-dihydro-3H- pyrazolo[3,4-c]isoquinolin-9-yl)ethyl)amino)-JV-methyl-[2,3' -bipyridine]-6'-carboxamide;

9-( 1 -((6-chloro-2-( 1 -methyl- 1 H- 1 ,2,4-triazol-3 -yl)pyridin-3 -yl)amino)ethyl)-4- cyclopropyl-3-ethyl-7-methyl-3,4-dihydro-5H-pyrazolo[3,4-c]i soquinolin-5-one;

9-[l-[[6-chloro-2-(l-methyl-6-oxo-3-pyridyl)-3-pyridyl]am ino]ethyl]-4-cyclopropyl- 3 -ethyl-7 -methyl -pyrazolo [3 ,4-c] isoquinolin-5 -one ; 9-[l-[[6-chloro-2-(l-methyl-l,2,4-triazol-3-yl)-3-pyridyl]am ino]ethyl]-3-ethyl-7- methyl -4 -tetrahydrofuran -3 -yl-pyrazolo [3,4 -c] isoquinolin-5 -one ;

9-[l-[[6-chloro-2-(l-methyl-l,2,4-triazol-3-yl)-3-pyridyl ]amino]ethyl]-3-ethyl-4-(2- hydroxy ethyl) -7 -methyl -pyrazolo [3 ,4-c] isoquinolin-5 -one ;

4-(6-chloro-3-(((3-ethyl-4,7-dimethyl-5-oxo-4,5-dihydro-3 H-pyrazolo[3,4- c]isoquinolin-9-yl)methyl)amino)pyridin-2-yl)-2-fluoro-JV-me thylbenzamide;

9-(l-((6-chloro-2-(3-methyl-lH-pyrazol-4-yl)pyridin-3-yl) oxy)ethyl)-3-ethyl-4,7- dimethyl-3 ,4 -dihydro -5H-py razol o [3,4 -c] isoquinolin-5 -one ;

3-(6-chloro-3-((l-(3-ethyl-7-methyl-4-(methyl-d3)-5-oxo-4 ,5-dihydro-3H- pyrazolo[3,4-c]isoquinolin-9-yl)ethyl)amino)pyridin-2-yl)-l, 2,4-oxadiazol-5(4H)-one;

9-(l-((6-chloro-2-(l-methyl-lH-l,2,4-triazol-3-yl)pyridin -3-yl)amino)ethyl)-3-ethyl-

4,7-dimethyl-3,4-dihydro-5H-pyrazolo[3,4-c]isoquinolin-5- one;

9-(l-((6-chloro-2-(4-methyl-4H-l,2,4-triazol-3-yl)pyridin -3-yl)amino)ethyl)-3-ethyl-

4,7-dimethyl-3,4-dihydro-5H-pyrazolo[3,4-c]isoquinolin-5- one;

9-(l-((6-chloro-2-(lH-tetrazol-5-yl)pyridin-3-yl)amino)et hyl)-3-ethyl-4,7-dimethyl- 3 ,4-dihydro -5H-pyrazolo [3,4 -c] isoquinolin-5 -one ;

9-(l-((6-chloro-2-(l-methyl-lH-l,2,3-triazol-5-yl)pyridin -3-yl)amino)ethyl)-3-ethyl-

4,7-dimethyl-3,4-dihydro-5H-pyrazolo[3,4-c]isoquinolin-5- one;

4-(6-chloro-3-((l-(3-ethyl-4,7-dimethyl-5-oxo-4,5-dihydro -3H-pyrazolo[3,4- c]isoquinolin-9-yl)ethyl)amino)pyridin-2-yl)benzoic acid;

4-(4-(6-chloro-3 -((1 -(3 -ethyl -4, 7 -dimethyl -5 -oxo-4,5 -dihydro-3H-pyrazolo [3 ,4- c] isoquinolin -9 -yl)ethyl)amino)pyridin-2 -yl) - 1 H-pyrazol- 1 -yl) -N,N -dimethylpiperidine - 1 - carboxamide;

3-(4-(4-(6-chloro-3-((l-(3-ethyl-4,7-dimethyl-5-oxo-4,5-d ihydro-3H-pyrazolo[3,4- c]isoquinolin-9-yl)ethyl)amino)pyridin-2-yl)- IH-pyrazol- 1 -yl)piperidin- 1 -yl)propanenitrile;

3 -ethyl -4, 7 -dimethyl-9-( 1 -((6-methyl-2-(3 -methyl- lH-pyrazol-4-yl)pyridin-3 - yl)amino)ethyl)-3,4-dihydro-5H-pyrazolo[3,4-c]isoquinolin-5- one;

9-( 1 -((6-chloro-2-( 1 -methyl- 1 H- 1 ,2,4-triazol-3 -yl)pyridin-3 -yl)amino)ethyl)-4-(2,2- difluoroethyl)-3 -ethyl-7 -methyl -3 ,4-dihydro-5H-pyrazolo [3 ,4-c] isoquinolin-5 -one ;

9-(l-((6-chloro-2'-methyl-[2,4'-bipyridin]-3-yl)amino)eth yl)-4-(2,2-difluoroethyl)-3- ethyl-7-methyl-3,4-dihydro-5H-pyrazolo[3,4-c]isoquinolin-5-o ne;

9-(l-((6-chloro-2-(pyrimidin-5-yl)pyridin-3-yl)amino)ethy l)-4-(2,2-difluoroethyl)-3- ethyl-7-methyl-3,4-dihydro-5H-pyrazolo[3,4-c]isoquinolin-5-o ne;

9-(l-((6-chloro-r-methyl-6'-oxo-r,6'-dihydro-[2,3'-bipyri din]-3-yl)amino)ethyl)-4- (2,2-difluoroethyl)-3 -ethyl-7 -methyl-3 ,4-dihydro-5H-pyrazolo [3 ,4-c] isoquinolin-5 -one; 6-chloro-3-((l-(4-(2,2-difluoroethyl)-3-ethyl-7-methyl-5-oxo -4,5-dihydro-3H- pyrazolo[3,4-c]isoquinolin-9-yl)ethyl)amino)-N-methyl-[2,3'- bipyridine]-6'-carboxamide;

9-[l-[[2-(l-acetyl-4-piperidyl)-6-chloro-3-pyridyl]amino] ethyl]-3-ethyl-4,7-dimethyl- pyrazolo [3 ,4-c]isoquinolin-5 -one ;

9-(l-((6-chloro-2-(l-methyl-lH-l,2,4-triazol-3-yl)pyridin -3-yl)amino)ethyl)-3-(2- hydroxyethyl)-4,7-dimethyl-3,4-dihydro-5H-pyrazolo[3,4-c]iso quinolin-5-one;

4-(6-chloro-3-(( 1 -(3 -(2-hydroxyethyl)-4,7 -dimethyl -5 -oxo-4,5-dihydro-3H- pyrazolo[3,4-c]isoquinolin-9-yl)ethyl)amino)pyridin-2-yl)-2- fluoro-N -methylbenzamide;

9-(l-((6-chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3-yl) amino)ethyl)-3-(2- hydroxyethyl)-4,7-dimethyl-3,4-dihydro-5H-pyrazolo[3,4-c]iso quinolin-5-one;

9-(l-((6-chloro-2-(4-hydroxypiperidin-l-yl)pyridin-3-yl)a mino)ethyl)-3-(2- hydroxyethyl)-4,7-dimethyl-3,4-dihydro-5H-pyrazolo[3,4-c]iso quinolin-5-one;

9-(l-((6-chloro-2-(quinolin-6-yl)pyridin-3-yl)amino)ethyl )-3-(2-hydroxyethyl)-4,7- dimethyl-3 ,4 -dihydro -5H-pyrazolo [3,4 -c] isoquinolin-5 -one ;

9-( 1 -((6-chloro-2-( 1 -methyl- 1 H-indazol-5 -yl)pyridin-3 -yl)amino)ethyl)-3 -(2- hydroxyethyl)-4,7-dimethyl-3,4-dihydro-5H-pyrazolo[3,4-c]iso quinolin-5-one;

9-(l-((6-chloro-2-(2-methyl-l-oxoisoindolin-5-yl)pyridin- 3-yl)amino)ethyl)-3-(2- hydroxyethyl)-4,7-dimethyl-3,4-dihydro-5H-pyrazolo[3,4-c]iso quinolin-5-one;

3 -(2 -hydroxy ethyl) -4,7 -dimethyl -9 -( 1 -((6-methyl -2 -( 1 -methyl - 1 H-pyrazol -4 - yl)pyridin-3-yl)amino)ethyl)-3,4-dihydro-5H-pyrazolo[3,4-c]i soquinolin-5-one;

9-(l-((6-chloro-2-(l,3,4-oxadiazol-2-yl)pyridin-3-yl)amin o)ethyl)-3-(2- hydroxyethyl)-4,7-dimethyl-3,4-dihydro-5H-pyrazolo[3,4-c]iso quinolin-5-one;

9-(l-((6-chloro-2-(2-methyl-2H-tetrazol-5-yl)pyridin-3-yl )amino)ethyl)-3-(2- hydroxyethyl)-4,7-dimethyl-3,4-dihydro-5H-pyrazolo[3,4-c]iso quinolin-5-one;

9-(l-((6-chloro-2-(pyrimidin-5-yl)pyridin-3-yl)amino)ethy l)-3-(2-hydroxyethyl)-4,7- dimethyl-3 ,4 -dihydro -5H-pyrazolo [3,4 -c] isoquinolin-5 -one ;

5-[6-chloro-3-[l-[3-(2-hydroxyethyl)-4,7-dimethyl-5-oxo-p yrazolo[3,4-c]isoquinolin-

9-yl]ethylamino] -2 -pyridyl] -N-methyl-pyridine-2 -carboxamide;

9-[l-[[6-chloro-2-(l-methyl-6-oxo-3-pyridyl)-3-pyridyl]am ino]ethyl]-3-(2- hydroxyethyl)-4,7-dimethyl-pyrazolo[3,4-c]isoquinolin-5-one;

9-[l-[[6-chloro-2-(l-methyl-2-oxo-4-pyridyl)-3-pyridyl]am ino]ethyl]-3-(2- hydroxyethyl)-4,7-dimethyl-pyrazolo[3,4-c]isoquinolin-5-one;

9-[l-[[6-chloro-2-(2-methoxy-4-pyridyl)-3-pyridyl]amino]e thyl]-3-(2 -hydroxyethyl)-

4,7-dimethyl-pyrazolo [3 ,4-c]isoquinolin-5 -one ; 9-[l-[[6-chloro-2-(l-methyl-4-piperidyl)-3-pyridyl]amino]eth yl]-3-(2-hydroxyethyl)- 4,7-dimethyl-pyrazolo [3 ,4-c]isoquinolin-5 -one ;

9-[l-[[2-(l-acetyl-4-piperidyl)-6-chloro-3-pyridyl]amino] ethyl]-3-(2-hydroxyethyl)- 4,7-dimethyl-pyrazolo [3 ,4-c]isoquinolin-5 -one ;

9-[l-[[6-chloro-2-(l-methyl-l,2,4-triazol-3-yl)-3-pyridyl ]amino]ethyl]-4-(2,2- difluoroethyl)-3-(2-hydroxyethyl)-7 -methyl -pyrazolo[3,4-c]isoquinolin-5-one;

5-[6-chloro-3-[l -[4-(2, 2-difluoroethyl)-3-(2-hydroxyethyl)-7 -methyl -5-oxo- pyrazolo[3,4-c]isoquinolin-9-yl]ethylamino]-2-pyridyl]-N-met hyl-pyridine-2 -carboxamide;

9-[l-[[6-chloro-2-(l-methyl-l,2,4-triazol-3-yl)-3-pyridyl ]amino]ethyl]-4-ethyl-3-(2- hydroxy ethyl) -7 -methyl -pyrazolo [3 ,4-c] isoquinolin-5 -one ;

9-[l-[(6-chloro-2-morpholino-3-pyridyl)amino]ethyl]-4-eth yl-3-(2-hydroxyethyl)-7- methyl -pyrazolo [3 ,4-c] isoquinolin-5 -one ;

9-[l-[[6-chloro-2-(l-methylpyrazol-4-yl)-3-pyridyl]amino] ethyl]-4,7-dimethyl-3-(2- morpholinoethyl)pyrazolo [3 ,4-c] isoquinolin-5 -one ;

9-( 1 -((6-chloro-3 '-fluoro-[2,4'-bipyridin] -3 -yl)amino)ethyl)-4,7 -dimethyl-3 -(2- morpholinoethyl)-3,4-dihydro-5H-pyrazolo[3,4-c]isoquinolin-5 -one;

9-(l-((6-chloro-3'-fluoro-[2,4'-bipyridin]-3-yl)amino)eth yl)-4,7-dimethyl-3-(2-(4- methylpiperazin- 1 -yl)ethyl)-3 ,4-dihydro-5H-pyrazolo [3 ,4-c] isoquinolin-5 -one ;

4-(6-chloro-3-((l-(4,7-dimethyl-3-(2-morpholinoethyl)-5-o xo-4,5-dihydro-3H- pyrazolo[3,4-c]isoquinolin-9-yl)ethyl)amino)pyridin-2-yl)-2- fluoro-N -methylbenzamide;

4-(6-chloro-3-((l-(4,7-dimethyl-3-(2-(4-methylpiperazin-l -yl)ethyl)-5-oxo-4,5- dihydro-3H-pyrazolo[3,4-c]isoquinolin-9-yl)ethyl)amino)pyrid in-2-yl)-2-fluoro-N- methylbenzamide ;

9-( 1 -((6-chloro-2-( 1 -methyl- 1 H- 1 ,2,4-triazol-3 -yl)pyridin-3 -yl)amino)ethyl)-4-(2,2- difluoroethyl)-3-(2-(5,6-dihydro-[l,2,4]triazolo[l,5-a]pyraz in-7(8H)-yl)ethyl)-7-methyl-3,4- dihydro-5H-pyrazolo [3 ,4-c] isoquinolin-5 -one ;

9-[l-[[6-chloro-2-(l-methyl-l,2,4-triazol-3-yl)-3-pyridyl ]amino]ethyl]-4-ethyl-7- methyl -3 -(2-pyridyl)pyrazolo [3 ,4-c] isoquinolin-5 -one ;

9-( 1 -((6-chloro-2-morpholinopyridin-3 -yl)amino)ethyl)-4-ethyl-7 -methyl -3 -(pyridin- 2-yl)-3 ,4-dihydro-5H-pyrazolo [3 ,4-c] isoquinolin-5 -one ;

6-chloro-3 -(( 1 -(4-ethyl-7-methyl-5 -oxo-3 -(pyridin-2-yl)-4,5 -dihydro-3H- pyrazolo[3,4-c]isoquinolin-9-yl)ethyl)amino)-N-methyl-[2,3'- bipyridine]-6'-carboxamide;

3-(6-chloro-3-((l-(4,7-dimethyl-5-oxo-3-(pyridin-2-yl)-4, 5-dihydro-3H-pyrazolo[3,4- c]isoquinolin-9-yl)ethyl)amino)pyridin-2-yl)-l,2,4-oxadiazol -5(4H)-one; 9-(l-((6-chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3-yl)ami no)ethyl)-4,7-dimethyl- 3-(pyridin-2-yl)-3,4-dihydro-5H-pyrazolo[3,4-c]isoquinolin-5 -one;

9-[l-[[6-chloro-2-(l-methyl-l,2,4-triazol-3-yl)-3-pyridyl ]amino]ethyl]-4-ethyl-7- methyl-3 -(3 -pyridyl)pyrazolo [3 ,4-c] isoquinolin-5 -one ;

9-[l-[[6-chloro-2-(l-methylpyrazol-4-yl)-3-pyridyl]amino] ethyl]-4,7-dimethyl-3-(2- pyridylmethyl)pyrazolo [3 ,4-c] isoquinolin-5 -one ;

3-(6-chloro-3-((l-(4,7-dimethyl-5-oxo-3-(pyridin-2-yhneth yl)-4,5-dihydro-3H- pyrazolo[3,4-c]isoquinolin-9-yl)ethyl)amino)pyridin-2-yl)-l, 2,4-oxadiazol-5(4H)-one;

9-[l-[[6-chloro-2-(l-methyl-l,2,4-triazol-3-yl)-3-pyridyl ]amino]ethyl]-4,7-dimethyl-

3 -( 1 -methyl-3 -piperidyl)pyrazolo [3,4 -c] isoquinolin-5 -one ;

9-[l-[[6-chloro-2-(l-methyl-l,2,4-triazol-3-yl)-3-pyridyl ]amino]ethyl]-3-[l-(2- hydroxy ethyl) -3 -piperidyl] -4, 7-dimethyl-pyrazolo [3 ,4-c] isoquinolin-5 -one ;

9-[ 1 -[ [6-chloro-2-( 1 -methylpyrazol-4-yl)-3 -pyridyl] amino] ethyl] -4,7-dimethyl-3 -( 1 - methyl-3 -piperidyl)pyrazolo [3 ,4-c] isoquinolin-5 -one;

9-[ 1 -[[6-chloro-2-( 1 -methylpyrazol-4-yl)-3 -pyridyl] amino] ethyl] -3 -[ 1 -(2- hydroxyethyl)-3 -piperidyl] -4, 7-dimethyl-pyrazolo [3 ,4-c] isoquinolin-5 -one ; methyl 3-[9-[l-[[6-chloro-2-(l-methylpyrazol-4-yl)-3-pyridyl]amino] ethyl]-4,7- dimethyl-5 -oxo -pyrazolo [3,4 -c] isoquinolin-3 -yl]piperidine - 1 -carboxylate ;

5-[6-chloro-3-[l-[4,7-dimethyl-3-(l-methyl-4-piperidyl)-5 -oxo-pyrazolo[3,4- c] isoquinolin -9 -yl] ethoxy] -2 -pyridyl] -N -methyl -pyridine -2 -carboxamide ;

5-[3-[l-[3-(l-acetyl-4-piperidyl)-4,7-dimethyl-5-oxo-pyra zolo[3,4-c]isoquinolin-9- yl]ethoxy]-6-chloro-2 -pyridyl] -N-methyl-pyridine-2 -carboxamide;

10-( 1 -((6-chloro-2-( 1 -methyl- 1H- 1 ,2,4-triazol-3 -yl)pyridin-3 -yl)amino)ethyl)-3 - (hydroxymethyl)-8-methyl-4,5-dihydro-3H,6H-2,2a,5a-triazaace anthrylen-6-one;

10-(l-((6-chloro-2-(2-methyl-2H-tetrazol-5-yl)pyridin-3-y l)amino)ethyl)-3-

(hydroxymethyl)-8-methyl-4,5-dihydro-3H,6H-2,2a,5a-triaza aceanthrylen-6-one;

10-( 1 -((6-chloro-2-( 1 ,3 ,4-oxadiazol-2-yl)pyridin-3 -yl)amino)ethyl)-3 - (hydroxymethyl)-8-methyl-4,5-dihydro-3H,6H-2,2a,5a-triazaace anthrylen-6-one;

10-( 1 -((6-chloro-2-( 1 -methyl- lH-pyrazol-4-yl)pyridin-3 -yl)amino)ethyl)-3 - (hydroxymethyl)-8-methyl-4,5-dihydro-3H,6H-2,2a,5a-triazaace anthrylen-6-one;

10-( 1 -((6-chloro-2-(3 -methyl- lH-pyrazol-4-yl)pyridin-3 -yl)amino)ethyl)-3 - (hydroxymethyl)-8-methyl-4,5-dihydro-3H,6H-2,2a,5a-triazaace anthrylen-6-one;

10-(l-((6-chloro-r-methyl-6'-oxo-r,6'-dihydro-[2,3'-bipyr idin]-3-yl)amino)ethyl)-3-

(hydroxymethyl)-8-methyl-4,5-dihydro-3H,6H-2,2a,5a-triaza aceanthrylen-6-one; 10-( 1 -((6-chloro-2-( 1 -methyl- 1H- 1 ,2,4-triazol-3 -yl)pyridin-3 -yl)amino)ethyl)-3 - ((dimethylamino)methyl)-8-methyl-4,5-dihydro-3H,6H-2,2a,5a-t riazaaceanthrylen-6-one;

10-(l-((6-chloro-2-(4-hydroxypiperidin-l-yl)pyridin-3-yl) amino)ethyl)-8-methyl-4,5- dihydro-3H,6H-2,2a,5a-triazaaceanthrylen-6-one;

1 -(6-chloro-3-(( 1 -(8-methyl-6-oxo-4,5 -dihydro-3 H,6H-2, 2a, 5a-triazaaceanthrylen- 10- yl)ethyl)amino)pyridin-2 -yl) -N -methylpiperidine -4 -carboxamide ;

10-(l-((2-(4-acetylpiperazin-l-yl)-6-chloropyridin-3-yl)a mino)ethyl)-8-methyl-4,5- dihydro-3H,6H-2,2a,5a-triazaaceanthrylen-6-one;

10-( 1 -((6-chloro-2-( 1 ,3,4-oxadiazol-2-yl)pyridin-3 -yl)amino)ethyl)-8-methyl-4,5- dihydro-3H,6H-2,2a,5a-triazaaceanthrylen-6-one;

3-(6-chloro-3-((l-(8-methyl-6 -oxo-4, 5 -dihydro-3 H,6H-2, 2a, 5a-triazaaceanthrylen-10- yl)ethyl)amino)pyridin-2 -yl) - 1 ,2,4 -oxadiazol-5 (4H) -one ;

10-( 1 -((6-chloro-2-( 1 -methyl- 1H- 1 ,2,4-triazol-3 -yl)pyridin-3 -yl)amino)ethyl)-8- methyl-4,5-dihydro-3H,6H-2,2a,5a-triazaaceanthrylen-6-one;

10-( 1 -((6-chloro-2-( 1 -(2-hydroxyethyl)- 1H- 1 ,2,4-triazol-3 -yl)pyridin-3 - yl)amino)ethyl)-8-methyl-4,5-dihydro-3H,6H-2,2a,5a-triazaace anthrylen-6-one;

10-(l-((6-chloro-2-(2-methyl-2H-tetrazol-5-yl)pyridin-3-y l)amino)ethyl)-8-methyl-

4,5-dihydro-3H,6H-2,2a,5a-triazaaceanthrylen-6-one;

10-(l-((6-chloro-2-(l-(tetrahydro-2H-pyran-4-yl)-lH-pyraz ol-4-yl)pyridin-3- yl)amino)ethyl)-8-methyl-4,5-dihydro-3H,6H-2,2a,5a-triazaace anthrylen-6-one;

10-(l-((6-chloro-2-(l-(2-hydroxyethyl)-lH-pyrazol-4-yl)py ridin-3-yl)amino)ethyl)-8- methyl-4,5-dihydro-3H,6H-2,2a,5a-triazaaceanthrylen-6-one;

10-(l-((6-chloro-2-(l-(l-methylpiperidin-4-yl)-lH-pyrazol -4-yl)pyridin-3- yl)amino)ethyl)-8-methyl-4,5-dihydro-3H,6H-2,2a,5a-triazaace anthrylen-6-one;

10-(l-((6-chloro-2-(l-(l-methylpiperidin-3-yl)-lH-pyrazol -4-yl)pyridin-3- yl)amino)ethyl)-8-methyl-4,5-dihydro-3H,6H-2,2a,5a-triazaace anthrylen-6-one;

10-( 1 -((6-chloro-2-( 1 -( 1 -methylpyrrolidin-3 -yl)- lH-pyrazol-4-yl)pyridin-3 - yl)amino)ethyl)-8-methyl-4,5-dihydro-3H,6H-2,2a,5a-triazaace anthrylen-6-one;

10-( 1 -((6-chloro-2-( 1 -( 1 -methylazetidin-3 -yl)-lH-pyrazol-4-yl)pyridin-3- yl)amino)ethyl)-8-methyl-4,5-dihydro-3H,6H-2,2a,5a-triazaace anthrylen-6-one;

4-(4-(6-chloro-3-((l-(8-methyl-6-oxo-4,5-dihydro-3H,6H-2, 2a,5a-triazaaceanthrylen-

10-yl)ethyl)amino)pyridin-2-yl)-lH-pyrazol-l-yl)-N,N-dime thylpiperidine-l -carboxamide;

10-( 1 -((6-chloro-2'-methyl-[2,4'-bipyridin] -3 -yl)amino)ethyl)-8-methyl-4,5 -dihydro- 3H, 6H-2,2a, 5 a-triazaaceanthrylen-6 -one ; 10-( 1 -((6-chloro-[2,3'-bipyridin] -3 -yl)amino)ethyl)-8-methyl-4,5 -dihydro-3H,6H- 2,2a,5a-triazaaceanthrylen-6-one;

IO-( I -((6-chloro-l'-mcthyl-6'-oxo-l'.6'-dihydro-|2.3'-bipyridin|- 3-yl)amino)cthyl)-8- methyl-4,5-dihydro-3H,6H-2,2a,5a-triazaaceanthrylen-6-one;

4-(6-chloro-3-((l-(8-methyl-6 -oxo-4, 5 -dihydro-3 H,6H-2, 2a, 5a-triazaaceanthrylen-10- yl)ethyl)amino)pyridin-2 -yl) -2 -fluoro-N -methylbenzamide ;

6-chloro-N -methyl -3-((l-(8-methyl -6 -oxo-4, 5-dihydro-3H,6H-2, 2a, 5a- triazaaceanthrylen-10-yl)ethyl)amino)-[2,3 '-bipyridine] -6'-carboxamide;

10-( 1 -((6-chloro-6'-methoxy-[2,3 '-bipyridin] -3 -yl)amino)ethyl)-8-methyl-4,5 - dihydro-3H,6H-2,2a,5a-triazaaceanthrylen-6-one;

10-( 1 -((6-chloro-2'-methoxy-[2,4'-bipyridin] -3 -yl)amino)ethyl)-8-methyl-4,5 - dihydro-3H,6H-2,2a,5a-triazaaceanthrylen-6-one;

10-(l-((6-chloro-2'-(dimethylamino)-[2,4'-bipyridin]-3-yl )amino)ethyl)-8-methyl-4,5- dihydro-3H,6H-2,2a,5a-triazaaceanthrylen-6-one;

10-( 1 -((6-chloro-2-(2 -methyl- 1 -oxoisoindolin-5 -yl)pyridin-3 -yl)amino)ethyl)-8- methyl-4,5-dihydro-3H,6H-2,2a,5a-triazaaceanthrylen-6-one;

10-(l-((6-chloro-2-(quinolin-6-yl)pyridin-3-yl)amino)ethy l)-8-methyl-4,5-dihydro- 3H, 6H-2,2a, 5 a-triazaaceanthrylen-6 -one ;

10-( 1 -((6-chloro-2-( 1 -methyl- lH-indazol-5 -yl)pyridin-3-yl)amino)ethyl)-8-methyl- 4,5 -dihydro -3H, 6H-2,2a, 5 a-triazaaceanthrylen-6 -one ;

2-fluoro-N-methyl-4-(6-methyl-3-((l-(8-methyl-6-oxo-4,5-d ihydro-3H,6H-2,2a,5a- triazaaceanthrylen-10-yl)ethyl)amino)pyridin-2-yl)benzamide;

8-methyl- 10-( 1 -((6-methyl-2-( 1 -(tetrahydro-2H-pyran-4-yl)-lH-pyrazol-4-yl)pyridin- 3-yl)amino)ethyl)-4,5-dihydro-3H,6H-2,2a,5a-triazaaceanthryl en-6-one;

10-( 1 -((2 -( 1 -(2-hydroxyethyl)-lH-pyrazol-4-yl)-6-methylpyridin-3-yl)amin o)ethyl)- 8-methyl-4,5-dihydro-3H,6H-2,2a,5a-triazaaceanthrylen-6-one;

10-(l-((2',6-dimethyl-[2,4'-bipyridin]-3-yl)amino)ethyl)- 8-methyl-4,5-dihydro-

3H, 6H-2,2a, 5 a-triazaaceanthrylen-6 -one ;

10-( 1 -((2 -( 1 -cyclopropyl- lH-pyrazol-4-yl)-6-methylpyridin-3-yl)amino)ethyl)-8- methyl-4,5-dihydro-3H,6H-2,2a,5a-triazaaceanthrylen-6-one;

7-(6-chloro-3-((l-(8-methyl-6 -oxo-4, 5-dihydro-3H,6H-2, 2a, 5a-triazaaceanthrylen-10- yl)ethyl)amino)pyridin-2-yl)hexahydro-3H-oxazolo[3,4-a]pyraz in-3-one;

10-( 1 -((6-chloro-2-(5 -methyl-4,5 ,6,7-tetrahydropyrazolo [ 1 ,5 -a]pyrazin-3 -yl)pyridin- 3-yl)amino)ethyl)-8-methyl-4,5-dihydro-3H,6H-2,2a,5a-triazaa ceanthrylen-6-one; 10-(l-((6-chloro-6'-((dimethylamino)methyl)-[2,3'-bipyridin] -3-yl)amino)ethyl)-8- methyl-4,5-dihydro-3H,6H-2,2a,5a-triazaaceanthrylen-6-one;

3-(3-((l-(3,8-dimethyl-6-oxo-4,5-dihydro-3H,6H-2,2a,5a-tr iazaaceanthrylen-10- yl)ethyl)amino)-6-methylpyridin-2-yl)-l,2,4-oxadiazol-5(4H)- one;

8-methyl- 10-( 1 -((6-methyl-2-(2-methyl-2H-tetrazol-5 -yl)pyridin-3-yl)amino)ethyl)- 4,5-dihydro-3H,6H-2,2a,5a-triazaaceanthrylen-6-one;

3-(3-((l-(3-ethyl-4,7-dimethyl-5-oxo-4,5-dihydro-3H-pyraz olo[3,4-c]isoquinolin-9- yl)ethyl)amino)-6-methylpyridin-2-yl)-l,2,4-oxadiazol-5(4H)- one;

10-( 1 -((6-chloro-2-( 1 -methyl- 1H- 1 ,2,4-triazol-3 -yl)pyridin-3 -yl)amino)-2- hydroxyethyl)-8-methyl-4,5-dihydro-3H,6H-2,2a,5a-triazaacean thrylen-6-one;

10-( 1 -((6-chloro-2-( 1 -methyl- lH-pyrazol-4-yl)pyridin-3-yl)amino)-2-hydroxyethyl)- 8-methyl-4,5-dihydro-3H,6H-2,2a,5a-triazaaceanthrylen-6-one;

10-( 1 -((6-chloro-2-( 1 ,5 -dimethyl- 1 H-py razol -4-yl )py ridi n-3 -yl)amino)-2- hydroxyethyl)-8-methyl-4,5-dihydro-3H,6H-2,2a,5a-triazaacean thrylen-6-one;

10-( 1 -((6-chloro-2-( 1 ,3,4-oxadiazol-2-yl)pyridin-3 -yl)amino)-2-hydroxyethyl)-8- methyl-4,5-dihydro-3H,6H-2,2a,5a-triazaaceanthrylen-6-one;

10-(l-((6-chloro-2-(2-methyl-2H-tetrazol-5-yl)pyridin-3-y l)amino)-2 -hydroxyethyl)- 8-methyl -4, 5-dihydro-3H,6H-2, 2a, 5a-triazaaceanthrylen-6 -one;

10-( 1 -((6-chloro-2-(2 -methyl- 1 -oxoisoindolin-5 -yl)pyridin-3 -yl)amino)-2- hydroxyethyl)-8-methyl-4,5-dihydro-3H,6H-2,2a,5a-triazaacean thrylen-6-one; and

9-(l-((6-chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3-yl) amino)-2-(4- methylpiperazin-l-yl)ethyl)-3-ethyl-4,7-dimethyl-3,4-dihydro -5H-pyrazolo[3,4-c]isoquinolin- 5 -one; or a pharmaceutically acceptable salt thereof.

In some embodiments, the present disclosure encompasses the recognition that provided compounds display certain desirable characteristics, e.g., as compared to other known compounds. For example, in some embodiments, provided compounds are more potent in one or more biochemical or cellular assays described herein, and/or have one or more other characteristics that make them more suitable for drug development, such as better selectivity for mutant PI3Ka over wide-type (WT) PI3Ka and/or better ADME (absorption, distribution, metabolism, and excretion) properties including but not limited to better permeability, cytotoxicity, hepatocyte stability, solubility, and/or plasma protein binding profdes, than other known compounds. In some embodiments, provided compounds display certain desirable characteristics in one or more assays described herein, e.g., compared to other known compounds. In some embodiments, provided compounds are provided and/or utilized in a salt form (e.g., a pharmaceutically acceptable salt form). Reference to a compound provided herein is understood to include reference to salts thereof, unless otherwise indicated.

It will be understood that, unless otherwise specified or prohibited by the foregoing definition of any of Formulae I-VIIb, embodiments of variables X, Y, Z, U, Ring A, L ^A , R ^A , R ^A1 , R ^AG , R ¹ , R ² , R ³ , R ⁴ , R ⁵ , L ⁵ , R ^5A , R ^5A1 , R ^5A11 , R ^5A12 , R ^5AG , R ^G1 , R, R ^m , and n, as defined above and described in classes and subclasses herein, apply to compounds of any of Formulae I-VIIb, both singly and in combination.

It will be understood that, unless otherwise specified or prohibited by the foregoing definition of any of Formulae I, II, and III, embodiments of variables X, Y, Z, U, Ring A, L ^A , R ^A , R ^A1 , R ^AG , R ¹ , R ² , R ³ , R ⁴ , R ⁵ , L ⁵ , R ^5A , R ^5A1 , R ^5A11 , R ^5A12 , R ^5AG , R ^G1 , R, R ^m , and n, as defined above and described in classes and subclasses herein, apply to compounds of any of Formulae I, II, and III, both singly and in combination.

It will be appreciated that throughout the present disclosure, unless otherwise indicated, reference to a compound of Formula I is intended to also include any of Formulae I, II, and III, and compound species of such formulae disclosed herein.

Preparing Provided Compounds

Compounds of the present disclosure, including salts thereof, can be prepared using known organic synthesis techniques and can be synthesized according to numerous possible synthetic routes, such as those in the schemes below. The schemes below provide general guidance in connection with preparing the compounds of the present disclosure. One skilled in the art would understand that the preparations shown in the schemes can be modified or optimized using general knowledge of organic chemistry to prepare various compounds of the present disclosure. Provided compounds may generally be made by the processes described in the ensuing schemes and examples.

In some embodiments, provided compounds are prepared according to the following Scheme:

wherein each of Hal ¹ , Hal ² , and Hal ³ is independently suitable halogen, and each of X, Y, Z, U, R ¹ , R ³ , R ⁵ , Ring A, L ^A , R ^A , and n is as defined above for Formula I, and described in classes and subclasses herein, both singly and in combination. Accordingly, in some embodiments, intermediate A-2 is prepared by a process comprising reacting compounds of Formula A-l with a suitable reagent (e.g., N-bromosuccinimide). In some embodiments, intermediate A-3 is prepared by a process comprising reacting intermediate A-2 under Sandmeyer reaction conditions. In some embodiments, intermediate A-4 is prepared by a process comprising reacting intermediate A-3 with a suitable reagent (e.g., oxalyl chloride or thionyl chloride). In some embodiments, intermediate A-6 is prepared by a process comprising contacting intermediate A-4 with compounds of Formula A-5 under suitable conditions. In some embodiments, intermediate A-7 is prepared by a process comprising reacting intermediate A-6 under suitable cyclization conditions (e.g., SNAr reaction or C-H activation). In some embodiments, intermediate A-9 is prepared by a process comprising reacting intermediate A-7 with compounds of Formula A-8. In some embodiments, compounds of Formula I are prepared by a process comprising reacting intermediate A-9 under suitable conditions (e.g., transition metal-catalyzed cross-coupling reactions).

In some embodiments, provided compounds are prepared according to the following

Scheme:

wherein each of Hal and Hal ¹ is a suitable halogen, M is a suitable reacting group (e.g., pinacol boronic ester group or tributyltin group), and each of X, Y, Z, U, R ¹ , R ³ , R ^5A , Ring A, L ^A , R ^A and n is as defined above for Formula I, and described in classes and subclasses herein, both singly and in combination. Accordingly, in some embodiments, intermediate B-2 is prepared by a process comprising contacting compounds of Formula A -7 and B-l under suitable conditions (e.g., metal -catalyzed cross-coupling reactions). In some embodiments, intermediate B-3 is prepared by a process comprising reacting intermediate B-2 under suitable reducing conditions. In some embodiments, intermediate B-4 is prepared by a process comprising reacting intermediate B-3 with a suitable reagent (e.g., PBn). In some embodiments, compounds of Formula B-5 are prepared by a process comprising reacting intermediate B-4 with suitable nucleophiles.

Compositions

The present disclosure also provides compositions comprising a compound provided herein with one or more other components. In some embodiments, provided compositions comprise and/or deliver a compound described herein (e.g., compounds of any of Formulae I, II, and III).

In some embodiments, a provided composition is a pharmaceutical composition that comprises and/or delivers a compound provided herein (e.g., compounds of any of Formulae I, II, and III) and further comprises a pharmaceutically acceptable carrier.

Pharmaceutical compositions typically contain an active agent (e.g., a compound described herein) in an amount effective to achieve a desired therapeutic effect while avoiding or minimizing adverse side effects. In some embodiments, provided pharmaceutical compositions comprise a compound described herein and one or more fdlers, disintegrants, lubricants, glidants, anti-adherents, and/or anti-statics, etc. Provided pharmaceutical compositions can be in a variety of forms including oral dosage forms, topical creams, topical patches, iontophoresis forms, suppository, nasal spray and/or inhaler, eye drops, intraocular injection forms, depot forms, as well as injectable and infusible solutions. Methods of preparing pharmaceutical compositions are well known in the art.

In some embodiments, provided compounds are formulated in a unit dosage form for ease of administration and uniformity of dosage. The expression “unit dosage form” as used herein refers to a physically discrete unit of an active agent (e.g., a compound described herein) for administration to a subject. Typically, each such unit contains a predetermined quantity of active agent. In some embodiments, a unit dosage form contains an entire single dose of the agent. In some embodiments, more than one unit dosage form is administered to achieve a total single dose. In some embodiments, administration of multiple unit dosage forms is required, or expected to be required, in order to achieve an intended effect. A unit dosage form may be, for example, a liquid pharmaceutical composition containing a predetermined quantity of one or more active agents, a solid pharmaceutical composition (e.g., a tablet, a capsule, or the like) containing a predetermined amount of one or more active agents, a sustained release formulation containing a predetermined quantity of one or more active agents, or a drug delivery device containing a predetermined amount of one or more active agents, etc.

Provided compositions may be administered using any amount and any route of administration effective for treating or lessening the severity of any disease or disorder described herein.

Uses

The present disclosure provides uses for compounds and compositions described herein. In some embodiments, provided compounds and compositions are for use in medicine (e.g., as therapy). In some embodiments, provided compounds and compositions are useful in treating a disease, disorder, or condition, wherein an underlying pathology is, wholly or partially, mediated by PI3Ka. In some embodiments, provided compounds and compositions are useful in research as, for example, analytical tools and/or control compounds in biological assays.

In some embodiments, the present disclosure provides methods of administering provided compounds or compositions to a subject in need thereof. In some embodiments, the present disclosure provides methods of administering provided compounds or compositions to a subject suffering from or susceptible to a disease, disorder, or condition associated with PI3Ka. In some embodiments, the present disclosure provides methods of administering provided compounds or compositions to a subject suffering from or susceptible to a disease, disorder, or condition, wherein an underlying pathology is, wholly or partially, mediated by PI3Ka.

In some embodiments, provided compounds are useful as PI3Ka inhibitors. In some embodiments, the present disclosure provides methods of inhibiting PI3Ka in a subject comprising administering a provided compound or composition. In some embodiments, the present disclosure provides methods of inhibiting PI3Ka in a biological sample comprising contacting the sample with a provided compound or composition.

In some embodiments, the present disclosure provides methods of treating a disease, disorder or condition associated with PI3Ka in a subject in need thereof, comprising administering to the subject a provided compound or composition. In some embodiments, a disease, disorder or condition is associated with mutation of PI3Ka. In some embodiments, the present disclosure provides methods of treating a disease, disorder or condition, wherein an underlying pathology is, wholly or partially, mediated by PI3Ka, in a subject in need thereof, comprising administering to the subject a provided compound or composition.

In some embodiments, the present disclosure provides methods of treating a variety of PI3Ka-dependent diseases and disorders. In some embodiments, the disease of disorder is a cancer (e.g., breast cancer, brain cancer, prostate cancer, endometrial cancer, gastric cancer, leukemia, lymphoma, sarcoma, colorectal cancer, lung cancer, ovarian cancer, skin cancer, and head and neck cancer). In some embodiments, the disease or disorder associated with PI3Ka includes, but is not limited to, CLOVES syndrome (congenital lipomatous overgrowth, vascular malformations, epidermal naevi, scoliosis/skeletal and spinal syndrome), PIK3CA- related overgrowth syndrome (PROS), endometrial cancer, breast cancer, esophageal squamous-cell cancer, cervical squamous-cell carcinoma, cervical adenocarcinoma, colorectal adenocarcinoma, bladder urothelial carcinoma, glioblastoma, ovarian cancer, non-small-cell lung cancer, esophagogastric cancer, nerve-sheath tumor, head and neck squamous-cell carcinoma, melanoma, esophagogastric adenocarcinoma, soft-tissue sarcoma, prostate cancer, fibrolamellar carcinoma, hepatocellular carcinoma, diffuse glioma, colorectal cancer, pancreatic cancer, cholangiocarcinoma, B-cell lymphoma, mesothelioma, adrenocortical carcinoma, renal non-clear-cell carcinoma, renal clear-cell carcinoma, germ-cell carcinoma, thymic tumor, pheochromocytoma, miscellaneous neuroepithelial tumor, thyroid cancer, leukemia, and encapsulated glioma.

Combination Therapy One or more additional therapeutic agents such as, for example, chemotherapeutics or other anti-cancer agents, anti-inflammatory agents, steroids, immunosuppressants, anesthetics (e.g., for use in combination with a surgical procedure), or other agents useful for treating diseases associated with PI3Ka can be used in combination with the compounds and salts provided herein. The agents can be combined with the present compounds in a single dosage form, or the agents can be administered simultaneously or sequentially as separate dosage forms.

Compounds described herein can be used in combination with one or more other kinase inhibitors for the treatment of diseases, such as cancer, that are impacted by multiple signaling pathways. For example, a combination can include one or more inhibitors of the following kinases for the treatment of cancer: Aktl, Akt2, Akt3, TGF-pR, Pim, PKA, PKG, PKC, CaM-kinase, phosphorylase kinase, CDK4/6, MEKK, ERK, MAPK, mTOR, EGFR, HER2, HER3, HER4, INS-R, IGF-1R, IR-R, PDGFaR, PDGF0R, CSFIR, KIT, FLK-II, KDR/FLK-1, FLK-4, flt-1, FGFR1, FGFR2, FGFR3, FGFR4, c-Met, Ron, Sea, TRKA, TRKB, TRKC, FLT3, VEGFR/Flt2, Flt4, EphAl, EphA2, EphA3, EphB2, EphB4, Tie2, Src, Fyn, Lek, Fgr, Btk, Fak, SYK, FRK, JAK, ABL, ALK and B-Raf. Additionally, the solid forms of the inhibitor as described herein can be combined with inhibitors of kinases associated with the PIK3/Akt/mT0R signaling pathway, such as PI3K, Akt (including Aktl, Akt2 and Akt3) and mTOR kinases.

For treating cancer and other proliferative diseases, compounds described herein can be used in combination with targeted therapies, including JAK kinase inhibitors (ruxolitinib, additional JAK1/2 and JAK 1 -selective, baricitinib or itacitinib), Pim kinase inhibitors (e.g., LGH447, INCB053914 and SGI-1776), PI3 kinase inhibitors including PI3K-delta selective and broad spectrum PI3K inhibitors (e.g., parsaclisib and INCB50797), PI3K-gamma inhibitors such as PI3K-gamma selective inhibitors, MEK inhibitors, CSFIR inhibitors (e.g., PLX3397 and LY3022855), TAM receptor tyrosine kinases inhibitors (Tyro-3, Axl, and Mer; e.g., INCB81776), angiogenesis inhibitors, interleukin receptor inhibitors, Cyclin Dependent kinase inhibitors (e.g., palbociclib, ribociclib, and abemaciclib), BRAF inhibitors, mTOR inhibitors, proteasome inhibitors (Bortezomib, Carfdzomib), HD AC -inhibitors (panobinostat, vorinostat), DNA methyl transferase inhibitors, dexamethasone, bromo and extra terminal family members inhibitors (for example, bromodomain inhibitors or BET inhibitors, such as OTX015, CPI-0610, INCB54329 or INCB57643), LSD1 inhibitors (e.g., GSK2979552, INCB59872 and INCB60003), estrogen receptor modulators (e.g., fulvestrant), androgen receptor modulators (e.g., enzalutamide), BCL2 inhibitors (e.g., venetoclax), hypoxiainducible factor-2 alpha inhibitors (e.g., belzutifan), exportin-1 (XPO-1) inhibitors (e.g., selinexor), KRAS inhibitors (e.g., sotorasib), arginase inhibitors (e.g., INCB1158), indoleamine 2,3-dioxygenase inhibitors (e.g., epacadostat, NLG919 or BMS-986205), PARP inhibiors (e.g., olaparib or rucaparib), and inhibitors of BTK such as ibrutinib.

For treating cancer and other proliferative diseases, compounds described herein can be used in combination with chemotherapeutic agents, agonists or antagonists of nuclear receptors, or other anti -proliferative agents. Compounds described herein can also be used in combination with a medical therapy such as surgery or radiotherapy, e.g., gamma-radiation, neutron beam radiotherapy, electron beam radiotherapy, proton therapy, brachytherapy, and systemic radioactive isotopes.

Examples of suitable chemotherapeutic agents include any of: abarelix, abiraterone, afatinib, aflibercept, aldesleukin, alemtuzumab, alitretinoin, allopurinol, altretamine, amidox, amsacrine, anastrozole, aphidicolon, arsenic trioxide, asparaginase, axitinib, azacitidine, bevacizumab, bexarotene, baricitinib, bendamustine, bicalutamide, bleomycin, bortezombi, bortezomib, brivanib, buparlisib, busulfan intravenous, busulfan oral, calusterone, camptosar, capecitabine, carboplatin, carmustine, cediranib, cetuximab, chlorambucil, cisplatin, cladribine, clofarabine, crizotinib, cyclophosphamide, cytarabine, dacarbazine, dacomitinib, dactinomycin, dalteparin sodium, dasatinib, dactinomycin, daunorubicin, decitabine, degarelix, denileukin, denileukin diftitox, deoxycoformycin, dexrazoxane, didox, docetaxel, doxorubicin, droloxafine, dromostanolone propionate, eculizumab, enzalutamide, epidophyllotoxin, epirubicin, epothilones, erlotinib, estramustine, etoposide phosphate, etoposide, exemestane, fentanyl citrate, filgrastim, floxuridine, fludarabine, fluorouracil, flutamide, fulvestrant, gefitinib, gemcitabine, gemtuzumab ozogamicin, goserelin acetate, histrelin acetate, ibritumomab tiuxetan, idarubicin, idelalisib, ifosfamide, imatinib mesylate, interferon alfa 2a, irinotecan, lapatinib ditosylate, lenalidomide, letrozole, leucovorin, leuprolide acetate, levamisole, lonafamib, lomustine, meclorethamine, megestrol acetate, melphalan, mercaptopurine, methotrexate, methoxsalen, mithramycin, mitomycin C, mitotane, mitoxantrone, nandrolone phenpropionate, navelbene, necitumumab, nelarabine, neratinib, nilotinib, nilutamide, niraparib, nofetumomab, oserelin, oxaliplatin, paclitaxel, pamidronate, panitumumab, panobinostat, pazopanib, pegaspargase, pegfilgrastim, pemetrexed disodium, pentostatin, pilaralisib, pipobroman, plicamycin, ponatinib, porfimer, prednisone, procarbazine, quinacrine, ranibizumab, rasburicase, regorafenib, reloxafme, revlimid, rituximab, rucaparib, ruxolitinib, sorafenib, streptozocin, sunitinib, sunitinib maleate, tamoxifen, tegafur, temozolomide, teniposide, testolactone, tezacitabine, thalidomide, thioguanine, thiotepa, tipifamib, topotecan, toremifene, tositumomab, trastuzumab, tretinoin, triapine, trimidox, triptorelin, uracil mustard, valrubicin, vandetanib, vinblastine, vincristine, vindesine, vinorelbine, vorinostat, veliparib, talazoparib, and zoledronate.

Methods for the safe and effective administration of most of these chemotherapeutic agents are known to those skilled in the art. In addition, their administration is described in the standard literature. For example, the administration of many of the chemotherapeutic agents is described in the “Physicians’ Desk Reference” (PDR, e.g., 1996 edition, Medical Economics Company, Montvale, NJ), the disclosure of which is incorporated herein by reference as if set forth in its entirety.

Example anti-inflammatory agents include, but are not limited to, aspirin, choline salicylates, celecoxib, diclofenac potassium, diclofenac sodium, diclofenac sodium with misoprostol, diflunisal, etodolac, fenoprofen, flurbiprofen, ibuprofen, ketoprofen, meclofenamate sodium, mefenamic acid, nabumetone, naproxen, naproxen sodium, oxaprozin, piroxican, rofecoxib, salsalate, sodium salicylate, sulindac, tolmetin sodium, and valdecoxib.

Example steroids include, but are not limited to, corticosteroids such as cortisone, dexamethasone, hydrocortisone, methylprednisolone, prednisolone, and prednisone.

Example immunosuppressants include, but are not limited to, azathioprine, chlorambucil, cyclophosphamide, cyclosporine, daclizumab, infliximab, methotrexate, and tacrolimus.

Example anesthetics include, but are not limited, to local anesthetics (e.g., lidocaine, procain, ropivacaine) and general anesthetics (e.g., desflurane, enflurane, halothane, isoflurane, methoxyflurane, nitrous oxide, sevoflurane, mmobarbital, methohexital, thiamylal, thiopental, diazepam, lorazepam, midazolam, etomidate, ketamine, propofol, alfentanil, fentanyl, remifentanil, buprenorphine, butorphanol, hydromorphone levorphanol, meperidine, methadone, morphine, nalbuphine, oxymorphone, pentazocine).

In some embodiments, the additional therapeutic agent is administered simultaneously with a compound or salt provided herein. In some embodiments, the additional therapeutic agent is administered after administration of the compound or salt provided herein. In some embodiments, the additional therapeutic agent is administered prior to administration of the compound or salt provided herein. In some embodiments, the compound or salt provided herein is administered during a surgical procedure. In some embodiments, the compound or salt provided herein is administered in combination with an additional therapeutic agent during a surgical procedure. As provided herein, the additional compounds, inhibitors, agents, etc. can be combined with the compounds provided herein in a single or continuous dosage form, or they can be administered simultaneously or sequentially as separate dosage forms.

Labeled Compounds and Assay Methods

Another aspect of the present invention relates to fluorescent dye, spin label, heavy metal or radio-labeled compounds of the invention that would be useful not only in imaging but also in assays, both in vitro and in vivo, for localizing and quantitating the PI3Ka enzyme in tissue samples, including human, and for identifying PI3Ka enzyme ligands by inhibition binding of a labeled compound. Accordingly, the present invention includes PI3Ka enzyme assays that contain such labeled compounds.

The present invention further includes isotopically -labeled compounds of the invention. An “isotopically” or “radio -labeled” compound is a compound of the invention where one or more atoms are replaced or substituted by an atom having an atomic mass or mass number different from the atomic mass or mass number typically found in nature (z. e. , naturally occurring). Suitable radionuclides that may be incorporated in compounds of the present invention include but are not limited to ² H (also written as D for deuterium), ³ H (also written as T for tritium), ⁿ C, ¹³ C, ¹⁴ C, ¹³ N, ¹⁵ N, ¹⁵ O, ¹⁷ O, ¹⁸ O, ¹⁸ F, ³⁵ S, ³⁶ C1, ⁸² Br, ⁷⁵ Br, ⁷⁶ Br, ⁷⁷ Br, ¹²³ 1, ¹²⁴ 1, ¹²⁵ I and ¹³¹ I. The radionuclide that is incorporated in the instant radio-labeled compounds will depend on the specific application of that radio-labeled compound. For example, for in vitro FGFR enzyme labeling and competition assays, compounds that incorporate ³ H, ¹⁴ C, ⁸² Br, ¹²⁵ 1 , ¹³¹ I, or ³⁵ S will generally be most useful. For radio-imaging applications ⁿ C, ¹⁸ F, ¹²⁵ I, ¹²³ I, ¹²⁴ I, ¹³¹ 1, ⁷⁵ Br, ⁷⁶ Br or ⁷⁷ Br will generally be most useful.

One or more constituent atoms of the compounds presented herein can be replaced or substituted with isotopes of the atoms in natural or non-natural abundance. In some embodiments, one or more atoms are replaced or substituted by deuterium. For example, one or more hydrogen atoms in a compound of the present disclosure can be replaced by deuterium atoms (e.g., one or more hydrogen atoms of a C1-6 alkyl group of Formula I can be optionally substituted with deuterium atoms, such as -CD3 being substituted for -CH3). In some embodiments, alkyl groups of the disclosed Formulas (e.g., the compound of any of Formulas I-VIIb) can be perdeuterated.

In some embodiments, the compound provided herein (e.g., the compound of any of Formulas I-VIIb), or a pharmaceutically acceptable salt thereof, comprises at least one deuterium atom. In some embodiments, the compound provided herein (e.g., the compound of any of Formulas I-VIIb), or a pharmaceutically acceptable salt thereof, comprises two or more deuterium atoms.

In some embodiments, the compound provided herein (e.g., the compound of any of Formulas I-VIIb), or a pharmaceutically acceptable salt thereof, comprises three or more deuterium atoms.

In some embodiments, for a compound provided herein (e.g., the compound of any of Formulas I-VIIb), or a pharmaceutically acceptable salt thereof, all of the hydrogen atoms are replaced by deuterium atoms (z.e., the compound is “perdeuterated”).

It is understood that a “radio-labeled ” or “labeled compound” is a compound that has incorporated at least one radionuclide. In some embodiments the radionuclide is selected from the group consisting of ³ H, ¹⁴ C, ¹²⁵ 1 , ³⁵ S and ⁸² Br.

Synthetic methods for including isotopes into organic compounds are known in the art (Deuterium Labeling in Organic Chemistry by Alan F. Thomas (New York, N.Y., Appleton-Century-Crofts, 1971; The Renaissance of H/D Exchange by Jens Atzrodt, Volker Derdau, Thorsten Fey and Jochen Zimmermann, Angew. Chem. Int. Ed. 2007, 7744-7765; The Organic Chemistry of Isotopic Labelling by James R. Hanson, Royal Society of Chemistry, 2011). Isotopically labeled compounds can be used in various studies such as NMR spectroscopy, metabolism experiments, and/or assays.

Substitution with heavier isotopes, such as deuterium, may afford certain therapeutic advantages resulting from greater metabolic stability, for example, increased in vivo half-life or reduced dosage requirements, and hence may be preferred in some circumstances, (see e.g., A. Kerekes et. al. J. Med. Chem. 2011, 54, 201-210; R. Xu et. al. J. Label Compd. Radiopharm. 2015, 58, 308-312). In particular, substitution at one or more metabolism sites may afford one or more of the therapeutic advantages.

A radio-labeled compound of the invention can be used in a screening assay to identify/evaluate compounds. In general terms, a newly synthesized or identified compound (i.e., test compound) can be evaluated for its ability to reduce binding of the radio-labeled compound of the invention to the PI3Ka enzyme. Accordingly, the ability of a test compound to compete with the radio-labeled compound for binding to the PI3Ka enzyme directly correlates to its binding affinity.

Kits

The present invention also includes pharmaceutical kits useful, for example, in the treatment or prevention of PI3Ka-associated diseases or disorders referred to herein which include one or more containers containing a pharmaceutical composition comprising a therapeutically effective amount of a compound of the invention. Such kits can further include, if desired, one or more of various conventional pharmaceutical kit components, such as, for example, containers with one or more pharmaceutically acceptable carriers, additional containers, etc., as will be readily apparent to those skilled in the art. Instructions, either as inserts or as labels, indicating quantities of the components to be administered, guidelines for administration, and/or guidelines for mixing the components, can also be included in the kit.

The invention will be described in greater detail by way of specific examples. The following examples are offered for illustrative purposes, and are not intended to limit the invention in any manner. Those of skill in the art will readily recognize a variety of non- critical parameters which can be changed or modified to yield essentially the same results. The compounds of the Examples were found to be inhibitors of PI3Ka as described below.

EXAMPLES

As described in the Examples below, in certain exemplary embodiments, compounds are prepared according to the following general procedures. It will be appreciated that, although the general methods depict the synthesis of certain compounds of the present disclosure, the following general methods and other methods known to one of ordinary skill in the art can be applied to all compounds and subclasses and species of each of these compounds, as described herein.

Intermediate 1: l-(3-Amino-6-chloropyridin-2-yl)piperidin-4-ol

To a solution of 6-chloro-2-fhioro-3-nitro-pyridine (100.00 mg, 0.57 mmol) in 3 mb of EtOH was added piperidin-4-ol (57.30 mg, 0.57 mmol), stirred at 45 °C for 1 h. To this mixture was added potassium 2-methylpropan-2-olate (127.13 mg, 1.13 mmol) and 4, 4, 5, 5- tetramethyl-2-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl)- l,3,2-dioxaborolane (431.54 mg, 1.70 mmol), After stirring at 80 °C for overnight, the mixture was diluted with water and extracted with EtOAc. The combined organics were washed with brine and dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with DCM and ethyl acetate to provide the desired product as a yellow solid (90 mg, 70%). LCMS calculated for C10H15CIN3O (M+H) ⁺ m/z = 228.1; found 228.1.

Intermediates 2-5.

Intermediates 2-5 in Table 1-1 were prepared similarly as described for Intermediate 1.

Table 1-1.

Intermediate 6: 6-Chloro-2-(l,3,4-oxadiazol-2-yl)pyridin-3-amine

To a solution of 3-amino-6-chloropicolinic acid (100 mg, 0.58 mmol) in 2 mL of DCM was added (N-isocyanoimino)triphenylphosphorane (175 mg, 0.58 mmol). After stirring at r.t. for overnight, the mixture was diluted with water and extracted with DCM. The combined organics were washed with brine and dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with DCM and ethyl acetate to provide the desired product as a yellow solid (84 mg, 74%). LCMS calculated for C7H6CIN4O (M+H) ⁺ m/z = 197.0; found 197.0.

Intermediate 7: 6-Chloro-2-(l-methyl-lH-l,2,4-triazol-3-yl)pyridin-3-amine

Step 1: 3-amino-6-chloro-N'-methylpicolinimidohydrazide

To a solution of 3-amino-6-chloro-pyridine-2 -carbonitrile (500 mg, 3.26 mmol) in 10 mL of EtOH was added methylhydrazine (1.03 mL, 19.54 mml) at r.t. After stirring at 100 °C for 24 h, the solvent was removed under vacuum to provide the desired product as a yellow solid (600 mg, 92%). LCMS calculated for C7H11CIN5 (M+H) ⁺ m/z = 200.1; found 200.1.

Step 2: 6-chloro-2-(l-methyl-lH-l , 2, 4-triazol-3-yl)pyridin-3-amine

To a solution of 3-amino-6-chloro-N'-methylpicolinimidohydrazide (600 mg, 3 mmol) was dissolved in 5 mL of formic acid. After stirring at 105 °C or overnight, the solvent was removed under vacuum. Then to the residue was added 5 mL of ammonium hydroxide solution in water and EtOH, the mixture was stirred for overnight at 100 °C. The mixture was diluted with water and extracted with EtOAc. The combined organics were washed with brine and dried with anhydrous sodium sulfate, fdtered, and concentrated under reduced pressure. The residue was precipitated in ether, fdtered and washed with ether to provide the desired product as a yellow solid (420 mg, 67%). LCMS calculated for CgHgClNs (M+H) ⁺ m/z = 210.1; found 210.1.

Intermediate 8: 2-(3-(3-Amino-6-chloropyridin-2-yl)-lH-l,2,4-triazol-l-yl)et han-l-ol

The titled compound was prepared using similar procedures as described for Intermediate 7 with 2-hydrazineylethan-l-ol replacing methylhydrazine in Step 1. LCMS calculated for C9H11CIN5O (M+H) ⁺ m/z = 240.1; found 240.1.

Intermediate 9: 6-Chloro-2-(4-methyl-4H-l,2,4-triazol-3-yl)pyridin-3-amine

4-methyl-l,2,4-triazole (120.16 mg, 1.45 mmol) was dissolved in 2 mL of THF and cooled to -78 °C. n-Butyllithium (578.44 uL, 1.45 mmol) was added via a syringe. After stirring for 10 min, dichlorozinc (2M THF solution, 761.10 uL, 1.45 mmol) was added. The reaction mixture was stirred at - 78 °C for 20 min, then warmed to room temperature. The resulting mixture was transferred via a syringe into a pressure flask which contained a mixture of 2-bromo-6-chloro-pyridin-3-amine (100.0 mg, 0.48 mmol), tetrakis(triphenylphosphine)palladium (55.70 mg, 0.05 mmol) and 3 mL of dioxane. The reaction mixture was heated at 120 °C in the sealed flask for 15h, and then cooled to r.t. EtOAc was added to quench the reaction. The resulting mixture was washed with water. The organic layer was concentrated and purified with silica gel column to give product as yellow solid (80 mg, 79%). LCMS calculated for CgHgClNs (M+H) ⁺ m/z = 210.1; found 210.1.

Intermediate 10: 6-Chloro-2-(lH-tetrazol-5-yl)pyridin-3-amine

To a solution of 3 -amino-6-chloro-pyridine-2 -carbonitrile (1.54 g, 10.00 mmol) in 20 mL DMF was added sodium azide (1.3 g, 20.00 mmol) and ammonia hydrochloride (1.7 g, 20.00 mmol) in one portion. After stirring at 120 °C for 12h, the solvent was removed under vacuum. The residue was dissolved in water and adjusted the pH to 2 by adding 10 % HC1. The precipitate was collected by fdtration to give product as white solid (1.8 g, 92%). LCMS calculated for CLILCIM, (M+H) ⁺ m/z = 197.0; found 197.0.

Intermediate 11 : 6-Chloro-2-(2-methyl-2H-tetrazol-5-yl)pyridin-3-amine

Step 1: 2-(6-chloro-2-(lH-tetrazol-5-yl)pyridin-3-yl)isoindoline-l, 3-dione

To a solution of 6-chloro-2-(lH-tetrazol-5-yl)pyridin-3-amine (Intermediate 10) (1.8 g, 9.16 mmol) in 10 mL of acetic acid was added isobenzofuran- 1, 3-dione (3.4 g, 22.89 mmol) at r.t. After stirring at 120 °C for overnight, the solvent was removed under vacuum to provide the desired product as a yellow solid (2.9 g, 97%). LCMS calculated for CiTLClNeCL (M+H) ⁺ m/z = 327.0; found 327.0.

Step 2: 2-(6-chloro-2-(2-methyl-2H-tetrazol-5-yl)pyridin-3-yl)isoind oline-l, 3-dione To a solution of 2-[6-chloro-2-(lH-tetrazol-5-yl)-3-pyridyl]isoindoline-l, 3-dione (3.00 g, 9.18 mmol) in 10 mL DMF was added iodomethane (0.86 mL, 1.96 g, 13.77 mmol) and dipotassium carbonate ( 3.81 g , 27.55 mmol). After stirring at r.t. for 2h, the mixture was diluted with water and extracted with EtOAc. The combined organics were washed with brine and dried with anhydrous sodium sulfate, fdtered, and concentrated under reduced pressure. The resulting mixture was purified by silica gel column chromatography, eluted with DCM and ethyl acetate to provide the desired product as yellow solid (540 mg, 17%) and another regioisomer as yellow solid (570 mg, 18%). LCMS calculated for CI ₅ HI ₀ C1N ₆ O ₂ (M+H) ⁺ m/z = 341.1; found 341.1.

Step 3: 6-chloro-2-(2-methyl-2H-tetrazol-5-yl)pyridin-3-amine

To a solution of 2-(6-chloro-2-(2-methyl-2H-tetrazol-5-yl)pyridin-3-yl)isoind oline- 1, 3-dione (540 mg, 1.58 mmol) was added 10 mL of hydrazine monohydrate. After stirring at 45 °C for 2 h, the resulting mixture was diluted with water. The precipitate was filtered and washed with water to give the product as white solid (330 mg, 100 %). LCMS calculated for C ₇ H ₈ CN ₆ (M+H) ⁺ m/z = 211.0; found 211.0.

Intermediate 12: l-(l-methylazetidin-3-yl)-4-(4,4,5,5-tetramethyl-l,3,2-dioxa borolan-2- yl)-lH-pyrazole

Dissolved tert-butyl 3-(4-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl)-lH-pyrazo l-l- yl)azetidine-l -carboxylate (500 mg, 1.43 mmol) in 30% TFA in DCM. After stirring at r.t. for Ih, the solvent was removed under vacuum to give product (360 mg, 100%). LCMS calculated for C12H21BN3O2 (M+H) ⁺ m/z = 250.2; found 250.2. Step 2: l-(l-methylazetidin-3-yl)-4-(4,4,5,5-tetramethyl-l,3,2-dioxa borolan-2-yl)-lH- pyrazole

To a solution of l-(azetidin-3-yl)-4-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan- 2- yl)pyrazole (50.00 mg, 0.20 mmol) in 1 mL of ACN and 0.5 mL AcOH was added formaldehyde (73.94 uL, 60.26 mg, 2.01 mmol) and sodium triacetoxyborohydride (212.69 mg, 1.00 mmol). After stirring at r.t. for 30 min, the mixture was quenched with water and extracted with EtOAc. The combined organics were washed with brine and dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The resulting mixture was purified by silica gel column chromatography, eluted with DCM and ethyl acetate to provide the desired product as white solid (43 mg, 81%). LCMS calculated for C13H23BN3O2 (M+H) ⁺ m/z = 264.2; found 264.2.

Intermediates 13-14.

Intermediates 13-14 in Table 1-2 were prepared similarly as described for

Intermediate 12.

Table 1-2.

Intermediate 15: N,N-Dimethyl-4-(4-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2- yl)-lH- pyrazol-l-yl)piperidine-l-carboxamide

To a solution of 4-(4-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl)-lH-pyrazo l-l- yl)piperidine (100 mg, 0.36 mmol) in 3 mL ACN was added dimethylcarbamic chloride (77 mg, 0.72 mmol) and triethylamine (0.15 mL, 1. 1 mmol). After stirring at r.t. for 30 min, the mixture was quenched with water and extracted with EtOAc. The combined organics were washed with brine and dried with anhydrous sodium sulfate, fdtered, and concentrated under reduced pressure. The resulting mixture was purified by silica gel column chromatography, eluted with DCM and ethyl acetate to provide the desired product as white solid (102 mg, 81%). LCMS calculated for C17H30BN4O3 (M+H) ⁺ m/z = 349.2; found 349.2.

Intermediate 16: 3-(4-(4-(4,4,5,5-Tetramethyl-l,3,2-dioxaborolan-2-yl)-lH-pyr azol-l- yl)piperidin-l-yl)propanenitrile

To a solution of 4-(4-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl)-lH-pyrazo l-l- yl)piperidine (50 mg, 0.18 mmol) in 1 mL ACN was added acrylonitrile (47 mg, 0.2 mmol). After stirring at r.t. for 30 min, the mixture was quenched with water and extracted with EtOAc. The combined organics were washed with brine and dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The resulting mixture was purified by silica gel column chromatography, eluted with DCM and ethyl acetate to provide the desired product as white solid (26 mg, 44%). LCMS calculated for C17H28BN4O2 (M+H) ⁺ m/z = 331.2; found 331.2. Intermediate 17: tert- Butyl 3-(3-amino-6-chloropyridin-2-yl)-6,7-dihydropyrazolo[l,5- a] pyr azine-5(4H)-carboxylate

Step 1: tert-butyl 3-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl)-6, 7-dihydropyrazolo[l,5- a]pyrazine-5 ( 4H) -carboxylate

Under nitrogen, to a mixture of tert-butyl 3-bromo-6,7-dihydropyrazolo[l,5- a]pyrazine-5(4H)-carboxylate (100 mg, 0.33 mmol) and bis(pinacolato)diboron (100.85 mg, 0.40 mmol) was added potassium acetate (65 mg, 0.66 mmol), Pd(dppf)C12 DCM (54.0 mg, 0.07 mmol) and anhydrous 1,4-dioxane (3.3 mL) at room temperature. The resulting mixture was heated at 100 °C for 3 hours. Upon completion of reaction, the mixture was cooled to room temperature, solvent was removed under reduced pressure. The residue was purified with silica gel chromatography, eluting with 0-100% ethyl acetate/hexanes to give desired product as white solid (75.7mg, 66%), UCMS calculated for C17H29BN3O4 (M+H) ⁺ m/z = 350.2; found: 350.1;

Step 2: tert-Butyl 3-(3-amino-6-chloropyridin-2-yl)-6, 7-dihydropyrazolo[l,5-a]pyrazine- 5 ( 4H) -carboxylate

At room temperature, to a mixture of tert-butyl 3-(4,4,5,5-tetramethyl-l,3,2- dioxaborolan-2-yl)-6,7-dihydropyrazolo[l,5-a]pyrazine-5(4H)- carboxylate (75.8 mg, 0.22 mmol) and 2-bromo-6-chloropyridin-3-amine (50.0 mg, 0.24 mmol) was added tetrakis(triphenylphosphine)palladinm(0) (55.7 mg, 0.05 mmol), cesium carbonate (157.1 mg, 0.48 mmol), and 5 to 1 mixture of 1,4-dioxane and water (2.4 mL). The resulting mixture was heated at 80 °C for 1 hour. Upon completion of reaction, the mixture was cooled to room temperature. Solvent was removed under reduced pressure. The residue was purified with silica gel chromatography, eluting with 0-100% Ethyl acetate/Hexanes to give desired product as yellow solid (40.3 mg, 48%). UCMS calculated for C16H21CIN5O2 (M+H) ⁺ m/z = 350.1; found: 350.1;

Intermediate 18: 6-Chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3-amine

To a solution of 2-bromo-6-chloropyridin-3-amine (100 mg, 0.48 mmol) in 2.5 mL of dioxane and 0.5 mL of water was added (1 -methyl- lH-pyrazol-4-yl)boronic acid (73 mg, 0.58 mmol), potassium carbonate (133 mg, 0.96 mmol) and tetrakis(triphenylphosphine)palladium (111 mg, 0.1 mmol) under nitrogen. After stirring at 85 °C for 1 h, the mixture was diluted with water and extracted with EtOAc. The combined organics were washed with brine and dried with anhydrous sodium sulfate, fdtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with DCM and ethyl acetate to provide the desired product as a yellow solid (82 mg, 81%). LCMS calculated for C ₉ HI ₀ C1N ₄ (M+H) ⁺ m/z = 209. 1 ; found 209.1.

Intermediates 19-50.

Intermediates 19-34 in Table 1-3 were prepared similarly as described for

Intermediate 18.

Table 1-3.

Intermediate 51: l-(2-((Triisopropylsilyl)oxy)ethyl)-lH-pyrazol-5-amine

To a stirred solution of 2-(5-aminopyrazol-l-yl)ethanol (7.50 g, 58.99 mmol) in 100 mb DCM was added chloro(triisopropyl)silane (18.93 mL, 88.48 mmol), imidazole (8.03 g, 117.98 mmol) and N,N-dimethylpyridin-4-amine (1.08 g, 8.85 mmol) at 0 °C. The reaction was stirred at rt for 12h. LCMS analysis indicated the reaction was complete. The reaction was quenched with 100 mL water and extracted with 2 x 100 mL DCM. The combined organics were washed with saturated NaCl, dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with 0-10% EtOAc in DCM to provide the desired product (13.00 g, 77.7 %). LCMS calculated for Ci4H ₃ oN ₃ OSi (M+H) ⁺ m/z = 284.2; found 284.2.

Intermediate 52: 9-Bromo-4-cyclopropyl-3-ethyl-7-methyl-3,4-dihydro-5H-pyrazo lo [3,4- c]isoquinolin-5-one

Stepl : 3-bromo-N-cyclopropyl-2-iodo-5-methyl-benzamide

To a solution of 3 -bromo-2-iodo-5 -methyl -benzoic acid (2.0 g, 5.87 mmol) in 30 mb DMF was added cyclopropylamine (401.91 mg, 7.04 mmol) followed by HATU (3.3 g, 8.80 mmol) and JV-ethyl-JV-isopropyl-propan-2 -amine (2.04 mb, 11.73 mmol). The resulting solution was stirred at r.t. for Ih, then poured into a mixture of 200 mb EtOAc and 200 mb water. The aqueous phase was washed with 50 mb ethyl acetate 3 times. The combined organics were washed with sat. NaCl and dried with anhydrous sodium sulfate, fdtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with EtOAc/DCM (0 to 100%) to provide the desired product. LCMS calculated for CnH^BrlNO (M+H) ⁺ m/z = 379.9; found 379.9.

Step2: 9-bromo-4-cyclopropyl-3-ethyl-7-methyl-pyrazolo[3,4-c]isoqui nolin-5-one

To a 250 mL round bottom flask was added 3-bromo-A'-cyclopropyl-2-iodo-5- methyl -benzamide (1.80 g, 4.74 mmol) and l-ethyl-5-iodo-pyrazole (1.05 g, 4.74 mmol), followed by tris(2-furyl)phosphane (219.94 mg , 0.95 mmol), Pd(OAc)2 (106.34 mg , 0.47 mmol) and K2CO3 (1309.25 mg, 9.47 mmol). The flask was sealed with a rubber septum, evacuated and backfilled with nitrogen for three times. To this flask was added 90 mL DMF. The resulting solution was heated to 105 °C for 16 h under nitrogen atmosphere. Upon cooling to room temperature, the mixture was diluted with water and extracted with EtOAc three times. The combined organics were washed with sat. NaCl and dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with EtOAc/DCM (0 to 100%) to provide the desired product. LCMS calculated for C| ₍ ,HnBrN’,0 (M+H) ⁺ m/z = 346.1; found page 345.9

Intermediate 53: 9-Bromo-3-ethyl-7-methyl-4-(tetrahydrofuran-3-yl)-3,4-dihydr o-5H- pyrazolo [3,4-c] isoquinolin-5-one

The title compound was prepared using similar procedures as described for Intermediate 52 with tetrahydrofuran-3 -amine replacing cyclopropylamine in Step 1. The crude product was purified by silica gel column chromatography, eluted with EtOAc/DCM (0 to 100%) to provide the desired product. LCMS calculated for Ci7HigBrN3O2 (M+H) ⁺ m/z = 376.1; found page 376.1.

Intermediate 54: Benzyl 3-hydrazineylpiperidine-l-carboxylate

Step 1: benzyl 3-(2-(tert-butoxycarbonyl)hydrazineyl)piperidine-l-carboxyla te

To a mixture of benzyl 3 -oxopiperidine -1 -carboxylate (5.0 g, 21.4 mmol) was added MeOH (300 mL), tert-butyl hydrazinecarboxylate (2.9 g, 22 moml), and acetic acid (3 mL), and sodium cyanoborohydride (1.4 g, 22.8 mmol). The mixture was stirred for 12h at rt, then quenched sat. NaHCCh. The mixture was extracted with DCM/IPA 3: 1 for three times. The combined organics were washed with sat. NaCl and dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with DCM/MeOH to provide the desired product. LCMS calculated for C18H28N3O4 (M+H) ⁺ m/z = 350.2; found page 350.2

Step 2: benzyl 3 -hydrazinopiperidine-1 -carboxylate

To a stirred solution of benzyl 3-(2-tert-butoxycarbonylhydrazino)piperidine-l- carboxylate (15.00 g, 42.93 mmol) in 50 mL DCM was added 50 mL TFA at rt. The resulting mixture was kept stirred at rt overnight. LCMS analysis indicated the reaction was complete. The reaction mixture was concentrated under reduced pressure. The residue was used directly in the following step without further purification. LCMS calculated for C13H20N3O2 (M+H) ⁺ m/z = 250.2; found page 250.2.

Intermediates 55-56.

Intermediates 39-40 in Table 1-4 were prepared similarly as described for intermediate 38.

Table 1-4.

Preparation of Provided Compounds

Example 1: 2-((l-(4,7-dimethyl-5-oxo-4,5-dihydropyrazolo[l,5-a]quinazol in-9- yl)ethyl)amino)benzoic acid (1-1)

Step 1: 3-bromo-2-fluoro-5-methylbenzoyl chloride

To a mixture of 3 -bromo-2-fluoro-5 -methylbenzoic acid (1.0 g, 4.29 mmol) in toluene (100 mL) were added oxalyl dichloride (1.09 g, 8.58 mmol) and 0.1 mL DMF. The resulting mixture was stirred at 100 °C for 3 h. Upon cooling to room temperature, the reaction was concentrated under reduced pressure. The residue was used directly in next step without further purification.

Step 2: 9-bromo-7 -methylpyrazolo [1 ,5-a] ' quinazolin-5(4H)-one

The mixture of 3-bromo-2-fluoro-5-methylbenzoyl chloride (0.9 g, 3.6 mmol) in DMF (50 mL) was added lH-pyrazol-5-amine (427 mg, 5.15 mmol) and K2CO3 (1.18g, 8.58 mmol) at -10 °C. The reaction was then slowly warmed to rt and stirred for 30 min. After completion, the reaction was heated to 140 °C and stirred at this temperature for 6 h before quenched with water and extracted with EtOAc. The combined organics were removed partially, and the resulting solid was collected to afford pure product as a light brown solid (320 mg, 32%). LCMS calculated for CnH ₉ BrN ₃ O (M+H) ⁺ m/z = 278.0; found 278.1.

Step 3: 9-bromo-4, 7-dimethylpyrazolo [1 ,5-a] quinazolin-5(4H)-one

To a mixture of 9-bromo-7-methylpyrazolo[l,5-a]quinazolin-5(4H)-one (320 mg, 1.15 mmol) in DMF (20 mL) was added NaH (50 mg, 1.2 mmol) at 0 °C under nitrogen atmosphere. The resulting mixture was stirred at same temperature for 30 min Mel (213 mg, 1.5 mmol) was added. The reaction mixture was then slowly warmed to rt and stirred for 4 h. After completion, the reaction was carefully quenched with water at 0 °C while vigorously stirred. The mixture was then extracted with ethyl acetate (2 x 300 mL). The combined organics were dried with anhydrous sodium sulfate, fdtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with hexanes and ethyl acetate to provide the desired product, which was further purified by silica gel column chromatography, eluted with DCM and ethyl acetate to provide the desired product as a brown oil (210 mg, 65%). LCMS calculated for C12H11 BrN;O (M+H) ⁺ m/z = 292.0; found 292.0.

Step 4: 9-acetyl-4, 7-dimethylpyrazolo[l,5-a]quinazolin-5(4H)-one

The mixture of 9-bromo-4,7-dimethylpyrazolo[l,5-a]quinazolin-5(4H)-one (100 mg, 0.34 mmol), PdC12(PPh3)2 (23 mg, 0.03 mmol), and tributyl(l-ethoxyvinyl)stannane (143 mg, 0.4 mmol) in dioxane (2 mL) was heated at 100 °C for 12 h under nitrogen atmosphere. Upon cooling to room temperature, the mixture was added 2 N HC1 and stirred for 30 min. Then 200 mg CsF was added and stirred for another 30 min before the mixture was diluted with water and extracted with EtOAc. The combined organics were washed with sat. NaCl and dried with anhydrous sodium sulfate, fdtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with DCM and ethyl acetate to provide the desired product as a brown oil (60 mg, 69%). LCMS calculated for C14H14N3O2 (M+H) ⁺ m/z = 256.1; found 256.1.

Step 5: 9-(l-hydroxyethyl)-4, 7 -dimethylpyrazolo [1 ,5-a] ' quincizolin-5(4H)-one

To a mixture of 9-acetyl-4,7-dimethylpyrazolo[l,5-a]quinazolin-5(4H)-one (60 mg, 0.23 mmol) in THF (2 mL) was added LAH (0.3 mL, 0.3 mmol, 1 M solution in THF) at -78 °C. The resulting mixture was stirred at same temperature for 10 min, and then warmed to -40 °C before quenched with sat. NH4CI. The mixture was diluted with water and extracted with EtOAc. The combined organics were washed with sat. NaCl and dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with DCM and ethyl acetate to provide the desired product as a colorless oil (20 mg, 33%). LCMS calculated for C14H14N3O (M-0H) ⁺ m/z = 240.1; found 240.1.

Step 6: 9-(l-bromoethyl)-4, 7 -dimethylpyrazolo [1,5 -a] quinazolin- 5 (4H) -one

To a mixture of 9-(l-hydroxyethyl)-4,7-dimethylpyrazolo[l,5-a]quinazolin-5(4 H)- one (20 mg, 0.08 mmol) in DCM (1 mL) was added PBr3 (27 mg, 0.1 mmol). The resulting mixture was stirred at rt for 2 h. The mixture was then quenched with sat. NaHCCF and extracted with DCM. The combined organics were washed with sat. NaCl, dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was used directly in next step without further purification. Step 7: 2-((l-(4, 7-dimethyl-5-oxo-4,5-dihydropyrazolo[l,5-a]quinazolin-9- yl)ethyl)amino)benzoic acid (1-1)

9-(l-bromoethyl)-4,7-dimethylpyrazolo[l,5-a]quinazolin-5( 4H)-one (20 mg, 0.06 mmol) in 2 dram vial was added DMF (0.3 mL), and 2-aminobenzoic acid (20 mg, 0.14 mmol). The mixture was heated to 90 °C for 2 h. Upon cooling to room temperature, the mixture was diluted with MeOH and purified by prep-HPLC (column: Sunfire prep Cl 8 column, 30* 150 mm, 5μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C21H21N4O3 (M+H) ⁺ m/z = 377.1; found 377.2.

Example 2: 2-((l-(4,7-dimethyl-5-oxo-3-phenyl-4,5-dihydropyrazolo[l,5-a ]quinazolin-9- yl)ethyl)amino)benzoic acid (1-2)

Step 1: 9-bromo-3-iodo-7-methylpyrazolo[l,5-a]quinazolin-5(4H)-one

To a mixture of 9-bromo-7-methylpyrazolo[l,5-a]quinazolin-5(4H)-one (Example 1, step 2) (640 mg, 2.3 mmol) in DMF (20 mL) was added NIS (517 mg, 2.3 mmol) at 0 °C under nitrogen atmosphere. The reaction mixture was then slowly warmed to rt and stirred for 1 h. After completion, the reaction was diluted with ice water while stirred. The resulting solid was collected to afford pure product as a light brown solid (723 mg, 78%). LCMS calculated for CnHgBrlNsO (M+H) ⁺ m/z = 404.0; found 404.1.

Step 2: 9-bromo-3-iodo-4, 7-dimethylpyrazolo[l,5-a]quinazolin-5(4H)-one

To a mixture of 9-bromo-3-iodo-7-methylpyrazolo[l,5-a]quinazolin-5(4H)-one (442 mg, 1.10 mmol) in DMF (20 mL) was added NaH (47 mg, 1. 1 mmol) at 0 °C under nitrogen atmosphere. The resulting mixture was stirred at same temperature for 30 min Mel (200 mg, 1.3 mmol) was added. The reaction mixture was then slowly warmed to rt and stirred for 4 h. After completion, the reaction was carefully quenched with water at 0 °C while vigorously stirred. The mixture was then extracted with ethyl acetate (2 x 300 mL). The combined organics were dried with anhydrous sodium sulfate, fdtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with hexanes and ethyl acetate to provide the desired product, which was further purified by silica gel column chromatography, eluted with DCM and ethyl acetate to provide the desired product as a white solid (332 mg, 72%). LCMS calculated for C^HioBrlNsO (M+H) ⁺ m/z = 418.0; found 418.0.

Step 3: 9-bromo-4, 7-dimethyl-3-phenylpyrazolo[l,5-a]quinazolin-5(4H)-one

The mixture of 9-bromo-3-iodo-4,7-dimethylpyrazolo[l,5-a]quinazolin-5(4H)-o ne (200 mg, 0.48 mmol), Pd(PPh3)4 (57 mg, 0.05 mmol), phenylboronic acid (61 mg, 0.5 mmol), and K3PO4 (212 mg, 1 mmol) in dioxane (6 mL) and water (1 mL) was heated at 85 °C for 6 h under nitrogen atmosphere. Upon cooling to room temperature, the mixture was concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with DCM and ethyl acetate to provide the desired product as a brown solid (88 mg, 50%). LCMS calculated for Ci ₈ Hi ₅ BrN ₃ O (M+H) ⁺ m/z = 368.1; found 368.1.

Step 4: 9-acetyl-4, 7-dimethyl-3-phenylpyrazolo[l,5-a]quinazolin-5(4H)-one

The mixture of 9-bromo-4,7-dimethyl-3-phenylpyrazolo[l,5-a]quinazolin-5(4H) -one (88 mg, 0.24 mmol), PdC12(PPh3)2 (20 mg, 0.03 mmol), and tributyl(l-ethoxyvinyl)stannane (80 mg, 0.24 mmol) in dioxane (2 mL) was heated at 100 °C for 12 h under nitrogen atmosphere. Upon cooling to room temperature, the mixture was added 2 N HC1 and stirred for 30 min. Then 200 mg CsF was added and stirred for another 30 min before the mixture was diluted with water and extracted with EtOAc. The combined organics were washed with sat. NaCl, dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with DCM and ethyl acetate to provide the desired product as a brown oil (43 mg, 55%). LCMS calculated for C20H18N3O2 (M+H) ⁺ m/z = 332.1; found 332.1.

Step 5: 9-(l-hydroxyethyl)-4, 7 -dimethyl- 3 -phenylpyrazolo [1 ,5-a] ' quincizolin-5(4H)-one

To a mixture of 9-acetyl-4,7-dimethyl-3-phenylpyrazolo[l,5-a]quinazolin-5(4H )-one (43 mg, 0.13 mmol) in THF (4 mL) was added LAH (0.2 mL, 0.2 mmol, 1 M solution in THF) at -78 °C. The resulting mixture was stirred at same temperature for 10 min, and then warmed to -40 °C before quenched with sat. NH4CI. The mixture was diluted with water and extracted with EtOAc. The combined organics were washed with sat. NaCl, dried with anhydrous sodium sulfate, fdtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with DCM and ethyl acetate to provide the desired product as a colorless oil (13 mg, 30%). LCMS calculated for C20H18N3O (M- OH) ⁺ m/z = 316.1; found 316.1.

Step 6: 9-(l-bromoethyl)-4, 7-dimethyl-3-phenylpyrazolo[l,5-a]quinazolin-5(4H)-one

To a mixture of 9-(l-hydroxyethyl)-4,7-dimethyl-3-phenylpyrazolo[l,5-a]quina zolin- 5(4H)-one (13 mg, 0.04 mmol) in DCM (1 mL) was added PBrs (20 mg, 0.7 mmol). The resulting mixture was stirred at rt for 2 h. The mixture was then quenched with sat. NaHCCh and extracted with DCM. The combined organics were washed with sat. NaCl, dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was used directly in next step without further purification.

Step 7: 2-((l-(4, 7-dimethyl-5-oxo-3-phenyl-4,5-dihydropyrazolo [1 ,5-a] quinazolin-9- yl)ethyl)amino)benzoic acid (1-2)

9-( 1 -bromoethyl)-4,7-dimethyl-3 -phenylpyrazolo [ 1 ,5 -a] quinazolin-5 (4H)-one (14 mg, 0.035 mmol) in 2 dram vial was added DMF (0.3 mL), and 2-aminobenzoic acid (20 mg, 0. 14 mmol). The mixture was heated to 90 °C for 2 h. Upon cooling to room temperature, the mixture was diluted with MeOH and purified by prep-HPLC (column: Sunfire prep Cl 8 column, 30* 150 mm, 5μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C27H25N4O3 (M+H) ⁺ m/z = 453.2; found 453.3.

Example 3: 2-((l-(4,7-dimethyl-5-oxo-3-vinyl-4,5-dihydropyrazolo[l,5-a] quinazolin-9- yl)ethyl)amino)benzoic acid (1-3)

The titled compound was prepared using similar procedures as described for Example

2 with 4,4,5,5-tetramethyl-2-vinyl-l,3,2-dioxaborolane replacing phenylboronic acid in Step 3. The resulting mixture was purified by prep-HPLC (column: Sunfire prep Cl 8 column, 30* 150 mm, 5 μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C23H23N4O3 (M+H)+ m/z = 403.2; found 403.2.

Example 4: 2-((l-(4,7-Dimethyl-5-oxo-4,5-dihydroimidazo[l,5-a]quinazoli n-9- yl)ethyl)amino)benzoic acid (1-4)

Step 1 : 2-Amino-3-bromo-5-methylbenzoic acid

To a mixture of 2-amino-5-methylbenzoic acid (20 g, 132 mmol) in DMF (20 mL) was added NBS (23.4 g, 132 mmol) at 0 °C under nitrogen atmosphere. The reaction mixture was then slowly warmed to rt and stirred for 1 h. After completion, the reaction was diluted with ice water while stirred. The resulting solid was collected to afford the desired product as a light brown solid (25.2 g, 83%). LCMS calculated for CxHgBrNCF (M+H) ⁺ m/z = 230.0; found 230.1.

Step 2: 8-Bromo-6-methyl-2H-benzo[d] [1, 3 ] oxazine-2, 4(lH)-dione

To a mixture of 2-amino-3-bromo-5 -methylbenzoic acid (20 g, 87.3 mmol) in dioxane (300 mL) was added triphosgene (10.32 g, 34.9 mmol) at 0 °C. The resulting mixture was stirred at 100 °C for 2h. Upon cooling to room temperature, the resulting solid was collected to afford the desired product as a white solid, which was used in the next step without further purification. Step 3: 2-Amino-3-bromo-N,5-dimethylbenzamide

To a mixture of 8-bromo-6-methyl-2H-benzo[d][l,3]oxazine-2,4(lH)-dione (18 g, 70.8 mmol) in THF (300 mL) was added methylamine (35 mL, 2 M solution in THF) at 0 °C. The resulting mixture was warmed to rt, and then stirred for Ih. The solvent was removed, and crude product was used in next step without further purification.

Step 4: 8-Bromo-2-hydroxy-3,6-dimethylquinazolin-4(3H)-one

To the solid from Step 3 was added THF (300 mL) and triphosgene (10.32 g, 34.9 mmol) at rt. The resulting mixture was heated at 70 °C for 2h, upon cooling to room temperature, half of the solvent was removed under vacuum, the resulting solid was collected. The solid product was then washed with 4: 1 hexanes and ethyl acetate, then dried under vacuum overnight to afford pure product as a white solid, which was used in the next step without further purification.

Step 5: 8-Bromo-2-chloro-3,6-dimethylquinazolin-4(3H)-one

To a mixture of 8-bromo-2 -hydroxy-3, 6-dimethylquinazolin-4(3H)-one (16 g, 59.7 mmol) in POCL (83 mL, 891.88 mmoL) was added DIEA (40.45 mL, 237.8 mmol) at 0 °C. The resulting mixture was stirred at 120 °C for 12h in a pressure vessel. Upon cooling to room temperature, the mixture was slowly poured onto 2000 mL ice carefully. After 2h, the resulting solid was collected to afford a light brown solid, which was washed with water, sat NaHCCF then water. The solid was dried under vacuum to afford the desired product as light brown solid (13.6 g, 80%). Step 6: tert-Butyl 9-bromo-4, 7-dimethyl-5-oxo-4,5-dihydroimidazo[l,5-a]quinazoline-3- carboxylate

To a mixture of KO’Bu (2.9 g, 26.0 mmol) in dry DMF (100 mL) under nitrogen was added tert-butyl 2-isocyanoacetate (2.7 mL, 19.13 mmol) at 0 °C dropwise. The resulting mixture was stirred at 0 °C for 10 min before 8-bromo-2-chloro-3,6-dimethylquinazolin- 4(3H)-one (5 g, 17.3 mmol) was added in one portion. The resulting mixture was warmed to rt and stirred for 2h. After completion, the reaction was poured into 300 mL sat. NH4CI solution, then diluted with 200 mL water. The resulting solid was collected to afford pure product as a light brown solid, which was washed with water (2X). The solid was dried under vacuum to provide the desired product as beige solid (5.6 g, 85%). LCMS calculated for Ci7Hi ₉ BrN ₃ O3 (M+H) ⁺ m/z = 392.0; found 392.1.

Step 7: tert-Butyl 9-acetyl-4, 7-dimethyl-5-oxo-4,5-dihydroimidazo[l ,5-a] quinazoline-3- carboxylate

The mixture of tert-butyl 9-bromo-4,7-dimethyl-5-oxo-4,5-dihydroimidazo[l,5- a]quinazoline -3 -carboxylate (5.2 g, 13.2 mmol), PdC12(PPh3)2 (930 mg, 1.33 mmol), and tributyl(l-ethoxyvinyl)stannane (5.7 g, 15.9 mmol) in dioxane (150 mL) was heated at 100 °C for 8 h under nitrogen atmosphere. Upon cooling to room temperature, the mixture was added 2 N HC1 and stirred for 4 h. The mixture was diluted with water and extracted with EtOAc.

The combined organics were washed with sat. NaCl, dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with DCM and ethyl acetate to provide the desired product as a brown oil (4. 1 g, 88%). LCMS calculated for C19H22N3O4 (M+H) ⁺ m/z = 356.2; found 356.2. Step 8: tert-Butyl 9-(l-hydroxyethyl)-4, 7-dimethyl-5-oxo-4,5-dihydroimidazo[l,5- a] quinazoline-3-carboxylate tert-Butyl 9-acetyl-4,7-dimethyl-5-oxo-4,5-dihydroimidazo[l,5-a]quinazo line-3- carboxylate (5 g, 14 mmol) was dissolved in MeOH (100 mL) and DCM (100 mL) then NaBfL (551 mg, 14.58 mmol) was added at 0 °C in three portions under nitrogen. The resulting mixture was stirred at same temperature for 10 min before quenched with sat. NH4CI. The mixture was diluted with water and extracted with DCM. The combined organics were washed with sat. NaCl, and dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with DCM and MeOH (0 to 4%) to provide the desired product as a light yellow solid (3.5 g, 70%). LCMS calculated for C19H24N3O4 (M+H) ⁺ m/z = 358.2; found 358.2.

Step 9: tert-Butyl 9-(l-bromoethyl)-4,7-dimethyl-5-oxo-4,5-dihydroimidazo[l,5- a] quinazoline-3-carboxylate

To a mixture of tert-butyl 9-(l-hydroxyethyl)-4,7-dimethyl-5-oxo-4,5- dihydroimidazo[l,5-a]quinazoline-3-carboxylate (3.0 g, 8.4 mmol) in DCM (200 mL) was added PBr, (0.95 mL, 10 mmol) at 0 °C. The resulting mixture was warmed to rt and stirred for 2 h. The mixture was then quenched with sat. NaHCCh and extracted with DCM (2x). The combined organics were washed with sat. NaCl, and dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was used directly in next step without further purification.

Step 10: tert-Butyl 9-(l-((2-(methoxycarbonyl)phenyl)ammo)ethyl)-4, 7-dtmethyl-5-oxo-4,5- dihydroimidazo [1 ,5-a] quinazoline-3-carboxylate

To the solution of tert-butyl 9-(l-bromoethyl)-4,7-dimethyl-5-oxo-4,5- dihydroimidazo[l,5-a]quinazoline-3-carboxylate (3.2 g, 7.6 mmol) in DMF (100 mb) was added methyl 2-aminobenzoate (4.0 g, 26 mmol). The mixture was heated to 80 °C for 2 h. Upon cooling to room temperature, the mixture was diluted with water and extracted with ethyl acetate (3X). The combined organics were washed with water, sat. NaCl, and dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with hexanes and EtOAc (0 to 30%) to provide the desired product as a light yellow solid (2.7g, 73%). LCMS calculated for C27H31N4O5 (M+H) ⁺ m/z = 491.2; found 491.2.

Step 11: 9-(l-((2-(Methoxycarbonyl)phenyl)amino)ethyl)-4, 7-dimethyl-5-oxo-4,5- dihydroimidazo [1 ,5-a] quinazoline-3-carboxylic acid tert-Butyl 9-(l-((2-(methoxycarbonyl)phenyl)amino)ethyl)-4,7-dimethyl-5 -oxo-4,5- dihydroimidazo[l,5-a]quinazoline-3-carboxylate (1.7 g, 3.4 mmol) was dissolved in TFA (20 mb). The mixture was stirred for 3 h at rt. After completion, TFA was removed under vacuum. 200 mb of ice water and 50 mb of EtOAc were added to the reaction residue while stirring. After stirring for 20 min, the resulting solid was collected to afford a light brown solid, which was washed with water (2X). The solid was dried under vacuum to provide the pure product (1.2 g, 81%). LCMS calculated for C23H23N4O5 (M+H) ⁺ m/z = 435.2; found 435.2.

Step 12: Methyl 2-((l-(3-iodo-4, 7-dimethyl-5-oxo-4,5-dihydroimidazo[l,5-a]quinazolin-9- yl)ethyl)amino)benzoate

To a mixture of 9-(l-((2-(methoxycarbonyl)phenyl)amino)ethyl)-4,7-dimethyl-5 -oxo- 4,5-dihydroimidazo[l,5-a]quinazoline-3-carboxylic acid (1.2 g, 2.76 mmol) in DMF (20 mL) under nitrogen was added NaHCCh (928 mg, 11.05 mmol) at 0 °C, after stirring for 5 min, NIS (745 mg, 3.31 mmol) was added in one portion. The resulting mixture was warmed to rt and stirred for 2h. After completion, the reaction was quenched with sat. Na2S2Ch and diluted with water. The mixture was then extracted with EtOAc (3x). The combined organics were washed with water, sat. NaCl, and dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with hexanes and EtOAc (0 to 30%) to provide the desired product as a white solid (1.1 g, 74%). LCMS calculated for C22H22IN4O3 (M+H) ⁺ m/z = 517.1; found 517.1.

Step 13: Methyl 2-((l-(4, 7-dimethyl-5-oxo-4,5-dihydroimidazo[l,5-a]quinazolin-9- yl)ethyl)amino)henzoate

In a 2 mL drum vial, methyl 2-((l-(3-iodo-4,7-dimethyl-5-oxo-4,5- dihydroimidazo[l,5-a]quinazolin-9-yl)ethyl)amino)benzoate (20 mg, 0.04 mmol) and Pd/C (10 wt% on active carbon, 10 mg) was dissolved in MeOH (2 mL). The vial was then charged with latm H2 and stirred for 2h. The reaction mixture was then filtrate through a short pad of celite and solvent was removed under vacuum to afford the crude product which was used in next step without further purification.

Step 14: 2-((l-(4, 7-Dimethyl-5-oxo-4,5-dihydroimidazo[l,5-a]quinazolin-9- yl)ethyl)amino)henzoic acid To a mixture of methyl 2-((l-(4,7-dimethyl-5-oxo-4,5-dihydroimidazo[l,5- a]quinazolin-9-yl)ethyl)amino)benzoate (12 mg, 0.03 mmol) in THF (0.2 mL) and MeOH (0.5 mL) was added 0.5 mL of 2N NaOH solution. The mixture was heated at 75 °C for 20 min before neutralized by adding 2N HC1 solution. The mixture was diluted with MeOH and purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C21H21N4O3 (M+H) ⁺ m/z = 377.2; found 377.2.

Example 5: 2-((l-(3-Ethyl-4,7-dimethyl-5-oxo-4,5-dihydroimidazo[l,5-a]q uinazolin-9- yl)ethyl)amino)benzoic acid (1-5)

Step 1: Methyl 2-((l-(4, 7-dimethyl-5-oxo-3-vinyl-4,5-dihydroimidazo[l,5-a]quinazolin -9- yl)ethyl)amino)henzoate

A mixture of methyl 2-((l-(3-iodo-4,7-dimethyl-5-oxo-4,5-dihydroimidazo[l,5- a]quinazolin-9-yl)ethyl)amino)benzoate (Example 4, Step 12: 40 mg, 0.08 mmol), XphosG2- Pd (7 mg, 0.01 mmol), 4,4,5,5-tetramethyl-2-vinyl-l,3,2-dioxaborolane (15 mg, 0.1 mmol), and KjPCL ^l mg, 0.1 mmol) in dioxane (1 mL) and water (0.1 mL) was heated at 100 °C for 4 h under nitrogen atmosphere. Upon cooling to room temperature, the mixture was concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with hexanes and ethyl acetate to provide the desired product as a brown solid (23 mg, 71%). LCMS calculated for C24H25N4O3 (M+H) ⁺ m/z =417.2; found 417.2.

Step 2: Methyl 2-((l-(3-ethyl-4, 7-dimethyl-5-oxo-4,5-dihydroimidazo[l,5-a]quinazolin-9- yl)ethyl)amino)henzoate

In a 2 mL drum vial, methyl 2-((l-(4,7-dimethyl-5-oxo-3-vinyl-4,5- dihydroimidazo[l,5-a]quinazolin-9-yl)ethyl)amino)benzoate (23 mg, 0.06 mmol) and Pd/C (10 wt% on active carbon, 7 mg) was dissolved in MeOH (2 mL). The vial was then charged with latm H2 and stirred for 2h. The reaction mixture was then filtrate through a short pad of celite and solvent was removed under vacuum to afford the crude product which was used in next step without further purification.

Step 3: 2-((l-(3-Ethyl-4, 7-dimethyl-5-oxo-4,5-dihydroimidazo [1 ,5-a] quinazolin-9- yl)ethyl)amino)henzoic acid

To a mixture of methyl 2-((l-(3-ethyl-4,7-dimethyl-5-oxo-4,5-dihydroimidazo[l,5- a]quinazolin-9-yl)ethyl)amino)benzoate (17 mg, 0.04 mmol) in THF (0.2 mL) and MeOH ( 0.5 mL) was added 0.5 mL of 2N NaOH solution. The mixture was heated at 75 °C for 20 min before neutralized by adding 2N HC1 solution. The mixture was diluted with MeOH and purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C23H25N4O3 (M+H) ⁺ m/z = 405.2; found 405.2.

Example 6: 2-((l-(4,7-Dimethyl-5-oxo-3-(pyridin-2-yl)-4,5-dihydroimidaz o[l,5- a]quinazolin-9-yl)ethyl)amino)benzoic acid (1-6)

Step 1: Methyl 2-((l-(4, 7-dimethyl-5-oxo-3-(pyridin-2-yl)-4,5-dihydroimidazo [1 ,5- a]quinazolin-9-yl)ethyl)amino)henzoate

A mixture of methyl 2-((l-(3-iodo-4,7-dimethyl-5-oxo-4,5-dihydroimidazo[l,5- a]quinazolin-9-yl)ethyl)amino)benzoate (Example 4, Step 12: 20 mg, 0.04 mmol), Pd(PPh3)4 (11 mg, 0.01 mmol), 2-(tributylstannyl)pyridine (29 mg, 0.08 mmol), Cui (2 mg, 0.01 mmol) and CsF (24 mg, 0.16 mmol) in dioxane (1 mL) was heated at 100 °C for 8 h under nitrogen atmosphere. Upon cooling to room temperature, the mixture was concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with hexanes and ethyl acetate to provide the desired product as a brown solid (11 mg, 61%). LCMS calculated for C27H26N5O3 (M+H) ⁺ m/z =468.2; found 468.2.

Step 2: 2-((l-(4, 7-Dimethyl-5-oxo-3-(pyridin-2-yl)-4,5-dihydroimidazo[l,5-a]q uinazolin-9- yl)ethyl)amino)benzoic acid

To a mixture of methyl 2-((l-(4,7-dimethyl-5-oxo-3-(pyridin-2-yl)-4,5- dihydroimidazo[l,5-a]quinazolin-9-yl)ethyl)amino)benzoate (11 mg, 0.024 mmol) in THF (0.2 mL) and MeOH ( 0.5 mL) was added 0.5 mL of 2N NaOH solution. The mixture was heated at 75 °C for 20 min before 0.7 mL of 2N HC1 solution was added. The mixture was heated at 75 °C for 20 min before diluted with MeOH and purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5μm; mobile phase A: water (0. 1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C26H24N5O3 (M+H) ⁺ m/z = 454.2; found 454.2.

Example 7: 2-((l-(3,4,7-Trimethyl-5-oxo-4,5-dihydroimidazo[l,5-a]quinaz olin-9- yl)ethyl)amino)benzoic acid (1-7) The titled compound was prepared using similar procedures as described for Example 5 with methylboronic acid replacing 4,4,5,5-tetramethyl-2-vinyl-l,3,2-dioxaborolane in Step 1. The resulting mixture was purified by prep-HPLC (column: Sunfire prep Cl 8 column, 30* 150 mm, 5 μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C22H23N4O3 (M+H) ⁺ m/z = 391.2; found 391.2.

Example 8: 6-Methyl-3-((l-(3,4,7-trimethyl-5-oxo-4,5-dihydroimidazo[l,5 -a]quinazolin-

9-yl)ethyl)amino)picolinic acid (1-8)

Step 1: Methyl 3-((l-(3-iodo-4, 7-dimethyl-5-oxo-4,5-dihydroimidazo[l ,5-a] ' quinazolin-9- yl)ethyl)amino)-6-methylpicolinate

The titled compound was prepared using similar procedures as described for Example 4 with methyl 3-amino-6-methylpicolinate replacing methyl 2-aminobenzoate in Step 10. The product was purified by silica gel column chromatography, eluted with DCM and MeOH to provide the desired product as a brown solid. LCMS calculated for C22H23IN5O3 (M+H) ⁺ m/z =532.1; found 532.1.

Step 2: Methyl 6-methyl-3-((l-(3,4, 7-trimethyl-5-oxo-4,5-dihydroimidazo[l,5-a]quinazolin-9- yl)ethyl)amino)picolinate

A mixture of methyl 3-((l-(3-iodo-4,7-dimethyl-5-oxo-4,5-dihydroimidazo[l,5- a]quinazolin-9-yl)ethyl)amino)-6-methylpicolinate (40 mg, 0.08 mmol), XphosG2-Pd (7 mg, 0.01 mmol), methylboronic acid (18 mg, 0.3 mmol), and K3PO4 (40 mg, 0.2 mmol) in dioxane (1 mL) and water (0. 1 mL) was heated at 100 °C for 4 h under nitrogen atmosphere. Upon cooling to room temperature, the mixture was concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with hexanes and ethyl acetate to provide the desired product as a brown solid (19 mg, 60%). LCMS calculated for C23H26N5O3 (M+H) ⁺ m/z =420.2; found 420.2.

Step 3: 6-Methyl-3-((l-(3,4, 7-trimethyl-5-oxo-4,5-dihydroimidazo [1 ,5-a] quinazolin-9- yl)ethyl)amino)picolinic acid

To a mixture of methyl 6-methyl-3-((l-(3,4,7-trimethyl-5-oxo-4,5- dihydroimidazo[l,5-a]quinazolin-9-yl)ethyl)amino)picolinate (10 mg, 0.023 mmol) in THF (0.2 mL) and MeOH ( 0.5 mL) was added 0.5 mL of 2N NaOH solution. The mixture was heated at 75 °C for 20 min before neutralized by adding 2N HC1 solution. The mixture was diluted with MeOH and purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C22H24N5O3 (M+H) ⁺ m/z = 406.2; found 406.2.

Example 9: 2-((l-(7-Chloro-3-ethyl-4-methyl-5-oxo-4,5-dihydroimidazo[l, 5- a]quinazolin-9-yl)ethyl)amino)benzoic acid (1-9) The titled compound was prepared using similar procedures as described for Example 5 with methyl 2-((l-(7-chloro-3-iodo-4-methyl-5-oxo-4,5-dihydroimidazo[l,5 -a]quinazolin-9- yl)ethyl)amino)benzoate (prepared using similar procedures as described for Example 4) replacing methyl 2-((l-(3-iodo-4,7-dimethyl-5-oxo-4,5-dihydroimidazo[l,5-a]qu inazolin-9- yl)ethyl)amino)benzoate in Step 1. The resulting mixture was purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5 μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C22H22CIN4O3 (M+H) ⁺ m/z = 425. 1; found 425.2.

Example 10: 2-((l-(7-Chloro-4-methyl-5-oxo-4,5-dihydroimidazo[l,5-a]quin azolin-9- yl)ethyl)amino)benzoic acid (1-10)

The titled compound was prepared using similar procedures as described for Example 4 with methyl 2-amino-5-chlorobenzoic acid replacing 2-amino-5 -methylbenzoic acid in Step 1. The resulting mixture was purified by prep-HPLC (column: Sunfire prep Cl 8 column, 30* 150 mm, 5 μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C20H18CIN4O3 (M+H)+ m/z = 397.1; found 397.1.

Example 11: 2-((l-(7-Chloro-3,4-dimethyl-5-oxo-4,5-dihydroimidazo[l,5-a] quinazolin-9- yl)ethyl)amino)benzoic acid (I- 11)

The titled compound was prepared using similar procedures as described for Example 5 with methylboronic acid replacing 4,4,5,5-tetramethyl-2-vinyl-l,3,2-dioxaborolane, and methyl 2-((l-(7-chloro-3-iodo-4-methyl-5-oxo-4,5-dihydroimidazo[l,5 -a]quinazolin-9- yl)ethyl)amino)benzoate replacing methyl 2-((l-(3-iodo-4,7-dimethyl-5-oxo-4,5- dihydroimidazo[l,5-a]quinazolin-9-yl)ethyl)amino)benzoate in Step 1. The resulting mixture was purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5 μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C21H20CIN4O3 (M+H)+ m/z = 411.1; found 411.1.

Example 12: 2-((l-(7-Chloro-4-ethyl-5-oxo-4,5-dihydroimidazo[l,5-a]quina zolin-9- yl)ethyl)amino)benzoic acid (1-12)

The titled compound was prepared using similar procedures as described for Example 4 with 2-amino-5 -chlorobenzoic acid replacing 2-amino-5 -methylbenzoic acid in Step 1 and ethyl amine replacing methylamine in Step 3. The resulting mixture was purified by prep- HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5 μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C21H20CIN4O3 (M+H)+ m/z = 411.1; found 411.2.

Example 13: 2-((l-(7-Chloro-3,4-diethyl-5-oxo-4,5-dihydroimidazo[l,5-a]q uinazolin-9- yl)ethyl)amino)benzoic acid (1-13)

The titled compound was prepared using similar procedures as described for Example 5 with methyl 2-((l-(7-chloro-4-ethyl-3-iodo-5-oxo-4,5-dihydroimidazo[l,5- a]quinazolin-9- yl)ethyl)amino)benzoate replacing methyl 2-((l-(3-iodo-4,7-dimethyl-5-oxo-4,5- dihydroimidazo[l,5-a]quinazolin-9-yl)ethyl)amino)benzoate in Step 1. The resulting mixture was purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5 μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C23H24CIN4O3 (M+H) ⁺ m/z = 439.2; found 439.2.

Example 14: 2-((l-(4,7-Dimethyl-5-oxo-4,5-dihydroimidazo[l,2-a]quinazoli n-9- yl)ethyl)amino)benzoic acid (1-14)

Step 1: 9-Bromo-4, 7-dimethylimidazo[l,2-a]quinazolin-5(4H)-one

To a mixture of 8-bromo-2-chloro-3,6-dimethylquinazolin-4(3H)-one (Example 4, Step 5: 200 mg, 0.7 mmol) in DMF (1 mL) was added 2,2-dimethoxyethan-l -amine (219 mg, 2.1 mmol) under nitrogen atmosphere. The reaction mixture was heated at 145 °C for 12 h. After completion, the reaction was diluted with water and extracted with EtOAc. The combined organics were washed with water, sat. NaCl, and dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with DCM and MeOH (0 to 10%) to provide the desired product as a light yellow solid (85 mg, 42%). LCMS calculated for C12H1 iBrN;O (M+H) ⁺ m/z = 292.0; found 292.0.

Step 2: 9-Acetyl-4, 7-dimethylimidazo[l,2-a]quinazolin-5(4H)-one

A mixture of 9-bromo-4,7-dimethylimidazo[l,2-a]quinazolin-5(4H)-one (85 mg, 0.29 mmol), PdCl ₂ (PPh ₃ )2 (14 mg, 0.02 mmol), and tributyl(l-ethoxyvinyl)stannane (108 mg, 0.3 mmol) in dioxane (2 mL) was heated at 100 °C for 8 h under nitrogen atmosphere. Upon cooling to room temperature, the mixture was added 2 N HC1 and stirred for 4 h. The mixture was diluted with water and extracted with EtOAc. The combined organics were washed with sat. NaCl, dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with DCM and MeOH to provide the desired product as a brown oil (59 mg, 80%). LCMS calculated for C14H14N3O2 (M+H) ⁺ m/z = 256.1; found 256.2.

Step 3: 9-(l -Hydroxyethyl)-4, 7-dimethylimidazo[l,2-a]quinazolin-5(4H)-one

9-Acetyl-4,7-dimethylimidazo[l,2-a]quinazolin-5(4H)-one (59 mg, 0.23 mmol) was dissolved in THF (4 mL) and cooled down to -40 °C. LAH (IM THF solution, 0.23 mL) was added at dropwise. The resulting mixture was stirred at same temperature for 10 min before quenched with sat. NH4CI. The mixture was diluted with water and extracted with EtOAc. The combined organics were washed with sat. NaCl, and dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with DCM and MeOH (0 to 20%) to provide the desired product as a light yellow solid (23 mg 40%). LCMS calculated for C14H16N3O2 (M+H) ⁺ m/z = 258.1; found 258.1.

Step 4: 9-(l-Bromoethyl)-4, 7-dimethylimidazo [1 ,2-a] quinazolin-5(4H)-one

To a mixture of 9-(l-hydroxyethyl)-4,7-dimethylimidazo[l,2-a]quinazolin-5(4H )-one (23 mg, 0.09 mmol) in DCM (2 mL) was added PBr3 (56 mg, 0.2 mmol) at 0 °C. The resulting mixture was warmed to rt and stirred for 2 h. The mixture was then quenched with sat. NaHCOs and extracted with DCM (2x). The combined organics were washed with sat. NaCl, and dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was used directly in next step without further purification. Step 5: 2-((l-(4, 7-Dimethyl-5-oxo-4,5-dihydroimidazo[l,2-a]quinazolin-9- yl)ethyl)amino)benzoic acid

To a solution of 9-(l-bromoethyl)-4,7-dimethylimidazo[l,2-a]quinazolin-5(4H)- one (12 mg, 0.04 mmol) in DMF (0.5 mb) was added 2-aminobenzoic acid (30 mg, 0.22 mmol). The mixture was heated to 80 °C for 2 h. Upon cooling to room temperature, the mixture was diluted with MeOH and purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C21H21N4O3 (M+H) ⁺ m/z = 377.2; found 377.2.

Example 15: 2-((l-(4,7-Dimethyl-5-oxo-4,5-dihydro-[l,2,4]triazolo[l,5-a] quinazolin-9- yl)ethyl)amino)benzoic acid (1-15)

The titled compound was prepared using similar procedures as described for Example 1 with lH-l,2,4-triazol-5-amine replacing lH-pyrazol-5-amine in Step 2. The product was purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C20H20N5O3 (M+H) ⁺ m/z = 378.2; found 378.2.

Example 16: 2-((l-(3,4,7-Trimethyl-5-oxo-4,5-dihydro-3/7-pyrazolo[3,4-c] isoquinolin-9- yl)ethyl)amino)benzoic acid (1-16)

Step 1 : 3-Bromo-2-iodo-5-methyl-N-(l-methyl-lH-pyrazol-5-yl)benzamid e

To a mixture of 3 -bromo-2-iodo-5 -methylbenzoic acid (1.9 g, 5.7 mmol) in DMF (10 mL) was added HATU (2.35g, 6.2 mmol), l-methyl-lH-pyrazol-5-amine (500 mg, 5.2 mmol) and DIPEA (2.7 mL, 15.5 mmol). The reaction was stirred at r.t. for 4 h. After completion, the reaction was quenched with water. The mixture was then extracted with ethyl acetate (2 x 300 mL). The combined organics were dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure, which was further purified by silica gel column chromatography, eluted with DCM and ethyl acetate to provide the desired product as white solid (1.8 g, 83%). LCMS calculated for CAH^BrlbLO (M+H) ⁺ m/z = 419.9; found 419.9.

To a mixture of 3-bromo-2-iodo-5-mcthyl-N-( I -methyl- 1 //-pyrazol-5-yl (benzamide (459 mg, 1.10 mmol) in DMF (20 mL) was added NaH (47 mg, 1.1 mmol) at 0 °C under nitrogen atmosphere. The resulting mixture was stirred at same temperature for 30 min and then Mel (200 mg, 1.3 mmol) was added. The reaction mixture was then slowly warmed to rt and stirred for 1 h. After completion, the reaction was carefully quenched with water at 0 °C while vigorously stirred. The mixture was then extracted with ethyl acetate (2 x 300 mL). The combined organics were dried with anhydrous sodium sulfate, fdtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with hexanes and ethyl acetate to provide the desired product, which was further purified by silica gel column chromatography, eluted with DCM and ethyl acetate to provide the desired product as a white solid (370 mg, 78%). LCMS calculated for C nHuBrlbLO (M+H) ⁺ m/z = 433.9; found 434.0.

Step 3: 9-Bromo-3,4, 7-trimethyl-3,4-dihydro-5H-pyrazolo[3,4-c]isoquinolin-5-one

A mixture of 3 -bromo-2-iodo-N,5 -dimethyl -N-( 1 -methyl - 1 //-pyrazol -5 -yl)benzamide (148 mg, 0.34 mmol), Pd(PPh3)4 (196 mg, 0.17 mmol), KOAc (100 mg, 1 mmol) in DMAc (2 mL) was heated at 120 °C for 16 h under nitrogen atmosphere. Upon cooling to room temperature, the mixture was diluted with water and extracted with EtOAc. The combined organics were washed with sat. NaCl and dried with anhydrous sodium sulfate, fdtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with DCM and ethyl acetate to provide the desired product as a white solid (57 mg, 54%). LCMS calculated for CnHnBrN’.O (M+H) ⁺ m/z = 306.0; found 306.0.

Step 4: 9-Acetyl-3,4, 7-trimethyl-3,4-dihydro-5H-pyrazolo[3,4-c]isoquinolin-5-one

O

YYX

A, '=N'

A mixture of 9-bromo-3,4,7-trimethyl-3,4-dihydro-5H-pyrazolo[3,4-c]isoqui nolin-5- one (100 mg, 0.33 mmol), PdC12(PPh3)2 (23 mg, 0.03 mmol), and tributyl(l- ethoxyvinyl)stannane (143 mg, 0.4 mmol) in dioxane (2 mL) was heated at 100 °C for 2 h under nitrogen atmosphere. Upon cooling to room temperature, the mixture was added 2 N HC1 and stirred for 30 min. Then 200 mg CsF was added and stirred for another 30 min before the mixture was diluted with water and extracted with EtOAc. The combined organics were washed with sat. NaCl and dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with DCM and ethyl acetate to provide the desired product as a white solid (59 mg, 67%). LCMS calculated for C15H16N3O2 (M+H) ⁺ m/z = 270.1; found 270.1.

Step 5: 9-(l-Hydroxyethyl)-3, 4, 7 -trimethyl- 3, 4-dihydro-5H-pyrazolo[3, 4-c]isoquinolin-5-one To a mixture of 9-acetyl-3,4,7-trimethyl-3,4-dihydro-5H-pyrazolo[3,4-c]isoqu inolin- 5-one (60 mg, 0.22 mmol) in THF (2 mL) was added LAH (0.3 mL, 0.3 mmol, 1 M solution in THF) at -78 °C. The resulting mixture was stirred at same temperature for 10 min, and then warmed to -40 °C before quenched with sat. NH4CI. The mixture was diluted with water and extracted with EtOAc. The combined organics were washed with sat. NaCl and dried with anhydrous sodium sulfate, fdtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with DCM and ethyl acetate to provide the desired product as a white solid (55 mg, 92%). LCMS calculated for C15H18N3O2 (M+H) ⁺ m/z = 272.1; found 272.1.

Step 6: 9-(l-Bromoethyl)-3, 4, 7 -trimethyl- 3, 4-dihydro-5H-pyrazolo[3, 4-c]isoquinolin-5-one

To a mixture of 9-( 1 -hydroxyethyl)-3,4,7-trimethyl-3,4-dihydro-5H-pyrazolo[3,4- c]isoquinolin-5-one (20 mg, 0.07 mmol) in DCM (1 mL) was added PBrs (38 mg, 0.14 mmol). The resulting mixture was stirred at rt for 2 h. The mixture was then quenched with sat. NaHCCE and extracted with DCM. The combined organics were washed with sat. NaCl, dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was used directly in next step without further purification.

Step 7: 2-((l-(3,4, 7-Trimethyl-5-oxo-4,5-dihydro-3H-pyrazolo[3,4-c]isoquinolin- 9- yl)ethyl)amino)henzoic acid

9-( 1 -Bromoethyl)-3.4.7-tri methyl -3 ,4-dihydro-5//-py razolo 13 ,4-c | i soqui nol i n-5 -one (20 mg, 0.06 mmol) in 2 dram vial was added DMF (0.3 mL), and 2 -aminobenzoic acid (20 mg, 0. 14 mmol). The mixture was heated to 90 °C for 2 h. Upon cooling to room temperature, the mixture was diluted with MeOH and purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C22H23N4O3 (M+H) ⁺ m/z = 391.2; found 391.2

Example 17: 6-Chloro-3-((l-(3,4,7-trimethyl-5-oxo-4,5-dihydro-3/7-pyrazo lo[3,4- c]isoquinolin-9-yl)ethyl)amino)picolinic acid (1-17)

The titled compound was prepared using similar procedures as described for Example 16 with 3-amino-6-chloropyridine-2-carboxylic acid replacing 2 -aminobenzoic acid in Step 7. The resulting mixture was purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5 μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C21H21CIN5O3 (M+H) ⁺ m/z = 426.1; found 426.1.

Example 18: 2-((l-(3-Ethyl-4,7-dimethyl-5-oxo-4,5-dihydro-3/f-pyrazolo[3 ,4- c]isoquinolin-9-yl)ethyl)amino)benzoic acid (1-18)

The titled compound was prepared using similar procedures as described for Example 16 with 1 -ethyl-lH-pyrazol-5-amine replacing l-methyl-lH-pyrazol-5-amine in Step 1. The resulting mixture was purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5 μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C23H25N4O3 (M+H) ⁺ m/z = 405.2; found 405.2.

Example 19: 6-Chloro-3-((l-(3-ethyl-4,7-dimethyl-5-oxo-4,5-dihydro-3/f-p yrazolo[3,4- c]isoquinolin-9-yl)ethyl)amino)picolinic acid (1-19)

The titled compound was prepared using similar procedures as described for Example 16 with 1 -ethyl-lH-pyrazol-5-amine replacing l-methyl-lH-pyrazol-5-amine in Step 1 and 3- amino-6-chloropyridine-2 -carboxylic acid replacing 2-aminobenzoic acid in Step 7. The resulting mixture was purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5 μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C22H23CIN5O3 (M+H) ⁺ m/z = 440.2; found 440.2.

Example 20: 3-((l-(3-Ethyl-4,7-dimethyl-5-oxo-4,5-dihydro-3/f-pyrazolo[3 ,4- c]isoquinolin-9-yl)ethyl)amino)-6-methylpicolinic acid (1-20)

The titled compound was prepared using similar procedures as described for Example 16 with 1 -ethyl-lH-pyrazol-5-amine replacing l-methyl-lH-pyrazol-5-amine in Step 1 and 3- amino-6-methylpicolinic acid replacing 2 -aminobenzoic acid in Step 7. The resulting mixture was purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5 μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C23H26N5O3 (M+H) ⁺ m/z = 420.2; found 420.2.

Example 21: 2-((l-(4-Ethyl-3,7-dimethyl-5-oxo-4,5-dihydro-3/f-pyrazolo[3 ,4- c]isoquinolin-9-yl)ethyl)amino)benzoic acid (1-21)

The titled compound was prepared using similar procedures as described for Example 16 with iodoethane replacing iodomethane in Step 2. The resulting mixture was purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5 μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C23H25N4O3 (M+H) ⁺ m/z = 405.2; found 405.2.

Example 22: 6-Chloro-3-((l-(4-ethyl-3,7-dimethyl-5-oxo-4,5-dihydro-3/f-p yrazolo [3,4- c]isoquinolin-9-yl)ethyl)amino)picolinic acid (1-22)

The titled compound was prepared using similar procedures as described for Example 16 with iodoethane replacing iodomethane in Step 2 and 3-amino-6-chloropyridine-2- carboxylic acid replacing 2-aminobenzoic acid in Step 7. The resulting mixture was purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5 μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C22H23CIN5O3 (M+H) ⁺ m/z = 440.2; found 440.2.

Example 23: 2-((l-(3,4-Diethyl-7-methyl-5-oxo-4,5-dihydro-3/f-pyrazolo[3 ,4- c]isoquinolin-9-yl)ethyl)amino)benzoic acid (1-23) The titled compound was prepared using similar procedures as described for Example 16 with 1 -ethyl-lH-pyrazol-5-amine replacing l-methyl-lH-pyrazol-5-amine in Step 1 and iodoethane replacing iodomethane in Step 2. The resulting mixture was purified by prep- HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5 μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C24H27N4O3 (M+H) ⁺ m/z = 419.2; found 419.2.

Example 24: 2-((l-(3,4-Diethyl-7-methyl-5-oxo-4,5-dihydro-3/f-pyrazolo[3 ,4- c]isoquinolin-9-yl)ethyl)amino)-5-fluorobenzoic acid (1-24)

The titled compound was prepared using similar procedures as described for Example 16 with 1 -ethyl-lH-pyrazol-5-amine replacing l-methyl-lH-pyrazol-5-amine in Step 1, iodoethane replacing iodomethane in Step 2, and 2-amino-5 -fluorobenzoic acid replacing 2- aminobenzoic acid in Step 7. The resulting mixture was purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5 μm; mobile phase A: water (0. 1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C24H26FN4O3 (M+H) ⁺ m/z = 437.2; found 437.2.

Example 25: 3-((l-(3,4-Diethyl-7-methyl-5-oxo-4,5-dihydro-3/f-pyrazolo[3 ,4- c]isoquinolin-9-yl)ethyl)amino)-6-methylpicolinic acid (1-25)

The titled compound was prepared using similar procedures as described for Example 16 with 1 -ethyl-lH-pyrazol-5-amine replacing l-methyl-lH-pyrazol-5-amine in Step 1, iodoethane replacing iodomethane in Step 2, and 3-amino-6-methylpicolinic acid replacing 2- aminobenzoic acid in Step 7. The resulting mixture was purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5 μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C24H28N5O3 (M+H) ⁺ m/z = 434.2; found 434.2.

Example 26: 2-((l-(4,7-Dimethyl-5-oxo-2-propyl-4,5-dihydro-2/7-pyrazolo[ 3,4- c]isoquinolin-9-yl)ethyl)amino)benzoic acid (1-26)

Step 1. 3-bromo-2-iodo-N-(l-(4-methoxybenzyl)-lH-pyrazol-5-yl)-5-met hylbenzamide

To a solution of 3 -bromo-2-iodo-5 -methylbenzoic acid (25.83 g, 75.77 mmol) in DMF (250 mL) was added HATU (37.98 g, 100.0 mmol). The resulting mixture was stirred at room temperature for 15 minutes then 2-[(4-methoxyphenyl)methyl]pyrazol-3-amine (14.0 g, 68.9 mmol) and A.A-diisopropylcthylaminc (36.0 mL, 206.6 mmol) were added sequentially. The reaction mixture was stirred at room temperature for 24 hours and then poured into water (1500 mL). The resulting solid was collected via filtration, washed with water (500 mL), and dried. The resulting solid was then triturated with ethyl acetate (100 mL) and the suspension was stirred for 4 hours at room temperature. The suspension was filtered to afford the desired product as a yellow solid (22.0 g, 61% yield). LCMS calculated for CigHisBrlNsCL (M+H) ⁺ m/z = 526.0; found 526.0.

Step 2. 3-Bromo-2-iodo-N-(l-(4-methoxybenzyl)-lH-pyrazol-5-yl)-N,5-d imethylbenzamide To a solution of 3-bromo-2-iodo-/V-(l-(4-methoxybenzyl)-lH-pyrazol-5-yl)-5- methylbenzamide (9.0 g, 17.10 mmol) in DMF (150 mL) was added NaH (533.6 mg, 22.2 mmol) at 0 °C under nitrogen atmosphere. The resulting mixture was stirred at same temperature for 30 min and then Mel (3.40 g, 23.9 mmol) was added. The reaction mixture was then slowly warmed to rt and stirred for 4 h. After completion, the reaction was carefully quenched with water at 0 °C while vigorously stirred. The mixture was then extracted with ethyl acetate (2 x 400 mL). The combined organics were dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with hexanes and ethyl acetate to provide the desired product as a white solid (7.6 g, 82% yield). LCMS calculated for C2oH2oBrIN3C>2 (M+H) ⁺ m/z = 540.0; found 540.0.

Step 3. 9-Bromo-3-(4-methoxybenzyl)-4, 7 -dimethyl- 3, 4-dihydro-5H-pyrazolo [3,4- c ] isoquinolin-5-one

A mixture of 3-bromo-2-iodo-A-(l-(4-methoxybenzyl)-lH-pyrazol-5-yl)-A,5- dimethylbenzamide (400 mg, 0.74 mmol), Pd(PPh3)4 (427.8 mg, 0.37 mmol) and KOAc (436.0 mg, 4.44 mmol) in DMAc (6.5 mL) was heated at 120 °C for 16 h under nitrogen atmosphere. Upon cooling to room temperature, the mixture was diluted with water and extracted with EtOAc (2 x 30 mL). The combined organics were washed with sat. NaCl and dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with hexanes and ethyl acetate to provide the desired product (160 mg, 52 % yield). LCMS calculated for C ₂ oHi ₉ BrN302 (M+H) ⁺ m/z = 412.1; found 412.1.

Step 4. 9-Acetyl-3-(4-methoxybenzyl)-4, 7-dimethyl-3,4-dihydro-5H-pyrazolo[3,4- c ] isoquinolin-5-one

A mixture of 9-bromo-3-(4-methoxybenzyl)-4,7-dimethyl-3,4-dihydro-5H- pyrazolo[3,4-c]isoquinolin-5-one (660 mg, 1.60 mmol), Pd(PPh3)4 (370.0 mg, 0.32 mmol), copper iodide (30.5 mg, 0.16 mmol) and tributyl(l-ethoxyvinyl)stannane (1.16 g, 3.20 mmol) in dioxane (16 mL) was heated at 100 °C for 12 h under nitrogen atmosphere. Upon cooling to room temperature, the mixture was added 2 N HC1 and stirred for 30 min. Then 400 mg CsF was added and stirred for another 30 min before the mixture was diluted with water and extracted with EtOAc (2 x 80 mL). The combined organics were washed with sat. NaCl and dried with anhydrous sodium sulfate, fdtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with hexanes and ethyl acetate to provide the desired product (360 mg, 60% yield). LCMS calculated for C22H22N3O3 (M+H) ⁺ m/z = 376.2; found 376.2.

Step 5. 9-(l-Hydroxyethyl)-3-(4-methoxybenzyl)-4, 7-dimethyl-3,4-dihydro-5H-pyrazolo[3,4- c ] isoquinolin-5-one

To a mixture of 9-acetyl-3-(4-methoxybenzyl)-4,7-dimethyl-3,4-dihydro-5H- pyrazolo[3,4-c]isoquinolin-5-one (360 mg, 1.0 mmol) in THF (10 mL) was added LAH (2.0 mL, 2.0 mmol, 1 M solution in THF) at -78 °C. The resulting mixture was stirred at same temperature for 10 min, and then warmed to -40 °C before quenched with sat. NH4CI. The mixture was diluted with water and extracted with EtOAc (2 x 50 mL). The combined organics were washed with sat. NaCl and dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with hexanes and ethyl acetate to provide the desired product (220 mg, 58% yield). LCMS calculated for C22H24N3O3 (M+H) ⁺ m/z = 378.2; found 378.2. Step 6. 9-(l-Bromoethyl)-3-(4-methoxybenzyl)-4, 7-dimethyl-3,4-dihydro-5H-pyrazolo[3,4- c ] isoquinolin-5-one

To a mixture of 9-(l-hydroxyethyl)-3-(4-methoxybenzyl)-4,7-dimethyl-3,4-dihy dro- 5H-pyrazolo[3,4-c]isoquinolin-5-one (200 mg, 0.53 mmol) in DCM (20 mL) was added PBr3 (215.2 mg, 0.79 mmol). The resulting mixture was stirred at rt for 2 h. The mixture was then quenched with sat. NaHCO, and extracted with DCM. The combined organics were washed with sat. NaCl, dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was used directly in next step without further purification.

Step 7. Methyl 2-((l-(3-(4-methoxybenzyl)-4, 7-dtmethyl-5-oxo-4,5-dthydro-3H-pyrazolo[3,4- c]isoquinolin-9-yl)ethyl)amino)benzoate

To a solution of 9-(l-bromoethyl)-3-(4-methoxybenzyl)-4,7-dimethyl-3,4-dihydr o- 5H-pyrazolo[3,4-c]isoquinolin-5-one (198.2 mg, 0.45 mmol) in DMF (2.0 mL) was added methyl 2 -aminobenzoate (204.1 mg, 1.35 mmol) and the resulting reaction mixture was heated at 80 °C for 30 minutes. The mixture was diluted with water and extracted with EtOAc

(2 x 50 mL). The combined organics were washed with sat. NaCl and dried with anhydrous sodium sulfate, fdtered, and concentrated under reduced pressure. The residue was used directly in next step without further purification. LCMS calculated for C30H31N4O4 (M+H) ⁺ m/z = 511.2; found 511.2.

Step 8. Methyl 2-((l-(4, 7-dimethyl-5-oxo-4,5-dihydro-3H-pyrazolo[3,4-c]isoquinolin-9 - yl)ethyl)amino)benzoate

A solution of methyl 2-((l-(3-(4-methoxybenzyl)-4,7-dimethyl-5-oxo-4,5-dihydro- 3H-pyrazolo[3,4-c]isoquinolin-9-yl)ethyl)amino)benzoate (230 mg, 0.45 mmol) in TFA (5 ml) and DCM (5 mL) was stirred at 60 °C for 1 hour. The reaction mixture was concentrated under reduced pressure. The resulting solid was dissolved in sat. NaHCCL and extracted with ethyl acetate (2 x 50 mL). The combined organics were washed with sat. NaCl and dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with DCM and ethyl acetate to provide the desired product (140 mg, 80% yield). LCMS calculated for C22H23N4O3 (M+H) ⁺ m/z = 391.2; found 391.1.

Step 9. Methyl 2-((l-(4, 7-dimethyl-5-oxo-2-propyl-4,5-dihydro-2H-pyrazolo[3,4- c]isoquinolin-9-yl)ethyl)amino)benzoate

To a solution of methyl 2-((l-(4,7-dimethyl-5-oxo-4,5-dihydro-3H-pyrazolo[3,4- c]isoquinolin-9-yl)ethyl)amino)benzoate (20 mg, 0.05 mmol) was added 1 -iodopropane (17.4 mg, 0.1 mmol) and potassium carbonate (28.3 mg, 0.2 mmol). The reaction mixture was stirred at 60 °C for 8 hours. The mixture was diluted with water and extracted with EtOAc (2 x 25 mL). The combined organics were washed with sat. NaCl and dried with anhydrous sodium sulfate, fdtered, and concentrated under reduced pressure. The residue was used directly in next step without further purification. LCMS calculated for C25H29N4O3 (M+H) ⁺ m/z = 433.2; found 433.2.

Step 10. 2-((l-(4, 7-Dimethyl-5-oxo-2-propyl-4,5-dihydro-2H-pyrazolo[3,4-c]isoq uinolin-9- yl)ethyl)amino)henzoic acid

A solution of methyl 2-((l-(4,7-dimethyl-5-oxo-2-propyl-4,5-dihydro-2H- pyrazolo[3,4-c]isoquinolin-9-yl)ethyl)amino)benzoate (21.6 mg, 0.05 mmol) in THF (0.2 mL), MeOH (0.4 mL) and 2M NaOH aq. Solution (0.6 mL) was stirred at 75 °C for 2 hours. Upon cooling to room temperature, the mixture was diluted with MeOH and purified by prep- HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C24H27N4O3 (M+H) ⁺ m/z = 419.2; found 419.2.

Example 27: 2-((l-(4,7-Dimethyl-5-oxo-2-(tetrahydro-2/7-pyran-4-yl)-4,5- dihydro-2/7- pyrazolo[3,4-c]isoquinolin-9-yl)ethyl)amino)benzoic acid (1-27)

The titled compound was prepared using similar procedures as described for Example 26 with 4 -bromotetrahydropyran replacing 1 -iodopropane in Step 9. The resulting mixture was purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5 μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C26H29N4O4 (M+H) ⁺ m/z = 461.2; found 461.2.

Example 28: 2-((l-(4,7-Dimethyl-5-oxo-2-phenethyl-4,5-dihydro-2/7-pyrazo lo[3,4- c]isoquinolin-9-yl)ethyl)amino)benzoic acid (1-28)

The titled compound was prepared using similar procedures as described for Example 26 with (2-bromoethyl)benzene replacing 1-iodopropane in Step 9. The resulting mixture was purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5 μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid.

LCMS calculated for C29H29N4O3 (M+H) ⁺ m/z = 481.2; found 481.2.

Example 29: 2-((l-(2-(2-Fluoropyridin-4-yl)-4,7-dimethyl-5-oxo-4,5-dihyd ro-2/7- pyrazolo[3,4-c]isoquinolin-9-yl)ethyl)amino)benzoic acid (1-29)

Step 1. 2-((l-(3-(4-Methoxybenzyl)-4, 7-dimethyl-5-oxo-4,5-dihydro-3H-pyrazolo[3,4- c]isoquinolin-9-yl)ethyl)amino)benzoic acid

To a solution of 9-(l-bromoethyl)-3-(4-methoxybenzyl)-4,7-dimethyl-3,4-dihydr o- 5H-pyrazolo[3,4-c]isoquinolin-5-one (210 mg, 0.48 mmol) in DMF (2.0 mL) was added 2- aminobenzoic acid (197.4 mg, 1.44 mmol) and the resulting reaction mixture was heated at 80 °C for 60 minutes. The mixture was diluted with water and extracted with EtOAc (2 x 50 mL). The combined organics were washed with sat. NaCl and dried with anhydrous sodium sulfate, fdtered, and concentrated under reduced pressure. The residue was used directly in next step without further purification. LCMS calculated for C29H29N4O4 (M+H) ⁺ m/z = 497.2; found 497.2.

Step 2. 2-((l-(4, 7-Dimethyl-5-oxo-4,5-dihydro-3H-pyrazolo[3,4-c]isoquinolin-9 - yl)ethyl)amino)benzoic acid A solution of 2-((l-(3-(4-methoxybenzyl)-4,7-dimethyl-5-oxo-4,5-dihydro-3H - pyrazolo[3,4-c]isoquinolin-9-yl)ethyl)amino)benzoic acid (238.2 mg, 0.48 mmol) in TFA (5 ml) and DCM (5 mL) was stirred at 60 °C for 1 hour. The reaction mixture was concentrated under reduced pressure. The resulting solid was purified by silica gel column chromatography, eluted with DCM and ethyl acetate to provide the desired product (140 mg, 77% yield). LCMS calculated for C21H21N4O3 (M+H) ⁺ m/z = 377.2; found 377.1.

Step 3. 2-((l-(2-(2-Fluoropyridin-4-yl)-4, 7-dimethyl-5-oxo-4,5-dihydro-2H-pyrazolo[3,4- c]isoquinolin-9-yl)ethyl)amino)benzoic acid

A mixture of 2-((l-(4,7-dimethyl-5-oxo-4,5-dihydro-3H-pyrazolo[3,4-c]isoq uinolin- 9-yl)ethyl)amino)benzoic acid (15 mg, 0.04 mmol), copper acetate (29.0 mg, 0.16 mmol), pyridine (12.7 mg, 0.16 mmol) and (2-fhroropyridin-4-yl)boronic acid (22.6 mg, 0.16 mmol) in DCM (0.5 mL) was stirred at room temperature for 16 hours. Upon cooling to room temperature, the mixture was diluted with MeOH and purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5μm; mobile phase A: water (0. 1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C26H23FN5O3 (M+H) ⁺ m/z = 472.2; found 472.2.

Example 30: 2-((l-(4,7-Dimethyl-5-oxo-2-(pyrimidin-5-yl)-4,5-dihydro-2/7 -pyrazolo[3,4- c]isoquinolin-9-yl)ethyl)amino)benzoic acid (1-30)

The titled compound was prepared using similar procedures as described for Example 29 with pyrimidin-5-ylboronic acid replacing (2-fluoropyridin-4-yl)boronic acid in Step 3. The resulting mixture was purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5 μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C25H23N6O3 (M+H) ⁺ m/z = 455.2; found 455.1. Example 31: 6-Chloro-3-((l-(4,7-dimethyl-5-oxo-2-(o-tolyl)-4,5-dihydro-2 /f- pyrazolo [3,4-c] isoquinolin-9-yl)ethyl)amino)picolinic acid (1-31)

Step 1. 6-Chloro-3-((l-(3-(4-methoxybenzyl)-4, 7-dimethyl-5-oxo-4,5-dihydro-3H- pyrazolo[3, 4-c]isoquinolin-9-yl)ethyl)amino)picolinic acid

To a solution of 9-(l-bromoethyl)-3-(4-methoxybenzyl)-4,7-dimethyl-3,4-dihydr o- 5H-pyrazolo[3,4-c]isoquinolin-5-one (90 mg, 0.20 mmol) in DMF (1 mL) was added 3- amino-6-chloropicolinic acid (103.5 mg, 0.60 mmol) and the resulting reaction mixture was heated at 80 °C for 60 minutes. The mixture was diluted with water and extracted with EtOAc (2 x 50 mL). The combined organics were washed with sat. NaCl and dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was used directly in next step without further purification. LCMS calculated for C28H27CIN5O4 (M+H) ⁺ m/z = 532.2; found 532.1.

Step 2. 6-Chloro-3-((l-(4, 7-dimethyl-5-oxo-4,5-dihydro-3H-pyrazolo[3,4-c]isoquinolin-9 - yl)ethyl)amino)picolinic acid

A solution of 6-chloro-3-((l-(3-(4-methoxybenzyl)-4,7-dimethyl-5-oxo-4,5-d ihydro- 3H-pyrazolo[3,4-c]isoquinolin-9-yl)ethyl)amino)picolinic acid (106 mg, 0.20 mmol) in TFA (5 ml) and DCM (5 mL) was stirred at 60 °C for 1 hour. The reaction mixture was concentrated under reduced pressure. The resulting solid was purified by silica gel column chromatography, eluted with DCM and ethyl acetate to provide the desired product (49 mg, 60% yield). LCMS calculated for C20H19CIN5O3 (M+H) ⁺ m/z = 412.1; found 412.1.

Step 3. 6-Chloro-3-((l-(4, 7-dimethyl-5-oxo-2-(o-tolyl)-4,5-dihydro-2H-pyrazolo[3,4- c]isoquinolin-9-yl)ethyl)amino)picolinic acid

A mixture of 6-chloro-3-(( l-(4,7-dimethyl-5-oxo-4,5-dihydro-3H-pyrazolo[3,4- c]isoquinolin-9-yl)ethyl)amino)picolinic acid (16.5 mg, 0.04 mmol), copper acetate (29.0 mg, 0.16 mmol), pyridine (12.7 mg, 0.16 mmol) and o-tolylboronic acid (21.8 mg, 0.16 mmol) in DCM (0.5 mL) was stirred at room temperature for 16 hours. Upon cooling to room temperature, the mixture was diluted with MeOH and purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5μm; mobile phase A: water (0. 1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C27H25CIN5O3 (M+H) ⁺ m/z = 502.2; found 502.1.

Example 32: 2-[l-(3-Methoxy-5,8-dimethyl-6-oxo-benzo[c] [l,8]naphthyridin-10- yl)ethylamino] benzoic acid (1-32)

Step 1 : 2-Amino-3-bromo-5-methyl-benzoic acid

A mixture of 2-amino-5-methyl-benzoic acid, (20.00 g, 132.31 mmol) and 1- bromopyrrolidine-2, 5-dione, (25.90 g, 145.54 mmol) in N,N -dimethylformamide (180 mL) was stirred at room temperature for 3 hours. The reaction mixture was poured into water (500 mL) and the solid product was filtered off and washed with water to afford the title compound 2-amino-3-bromo-5-methyl-benzoic acid (29.00 g, 95.3 %). LCMS calculated for CxHgBrNCF (M+H) ⁺ m/z = 230.0/232.0; found 230.0/231.9.

Step 2: 3-Bromo-2-iodo-5-methyl-benzoic acid

2-Amino-3-bromo-5-methyl-benzoic acid (25 g, 108.6 mmol) was suspended in 120 mb water with stirring. The mixture was cooled in an ice bath and 100 mL concentrated sulfuric acid was added slowly. A solution of sodium nitrite, (13.50 g, 195.60 mmol) in 100 mL water was added dropwise over 0.5 hour. The reaction mixture was stirred for an additional 0.25 hour at 0-5°. The mixture was poured into a well-stirred solution of potassium iodide (27.06 g, 163.00 mmol) in 100 mL water. The resulting solid was collected by filtration and stirred in 200 mL ethyl acetate. A solution of 200 mL 20% sodium hydrogen sulfite was added slowly. The organic layer was separated, washed with 100 mL 20% sodium hydrogen sulfite, 50 mL saturated sodium chloride, dried (magnesium sulfate) and concentrated under reduced pressure. The residue was recrystallized from ether/hexane to provide 3-bromo-2-iodo-5-methyl-benzoic acid (22.00 g, 59.4 %).

Step 3: 3-Bromo-2-iodo-N,5-dimethyl-benzamide

To a stirred solution of 3 -bromo -2 -iodo -5 -methyl -benzoic acid (20.00 g , 58.66 mmol) in THF (250 mL) was added [dimethylamino(triazolo[4,5-b]pyridin-3- yloxy)methylene]-dimethyl-ammonium hexafluorophosphate (44.61 g , 117.32 mmol) and N- ethyl-N-isopropyl-propan-2 -amine (18.39 mL, 105.59 mmol) and the resulting mixture was kept stirred for 15 min. methanamine (3.64 g , 117.32 mmol) was then added to the reaction mixture and the resulting mixture was kept stirred at rt overnight. LCMS analysis indicated the reaction was complete. The reaction was quenched with water (200 mL) and THF was partially removed. The suspension was stirred for 30 min until precipitate formed. The precipitate was collected by filtration and washed with water to afford 3-bromo-2-iodo-N,5- dimethyl-benzamide (18.80 g, 90.5 %). LCMS calculated for CgHioBrINO (M+H) ⁺ m/z = 353.9/355.9; found 354.0/356.0.

Step 4: 10-Bromo-3-fluoro-5, 8-dimethyl-benzo[c] [1, 8 ]naphthyridin-6-one

A solution of 3-bromo-2-iodo-N,5-dimethyl -benzamide (10.00 g, 28.25 mmol), (2,6- difluoro-3-pyridyl)boronic acid (8.08 g , 50.85 mmol), dicesium carbonate (23.01 g , 70.62 mmol) and palladium triphenylphosphine (7.83 g , 6.78 mmol) in 100 mb toluene, 50 mb EtOH and 50 mb H2O was bubbled with N2 for 5 min. And the resulting reaction mixture was stirred at 70 °C overnight. LCMS analysis indicated the reaction was complete. The mixture was then extracted with ethyl acetate (2 x 150 mb). The combined organics were dried with anhydrous sodium sulfate, fdtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with hexanes, DCM and ethyl acetate to provide the desired product 10-bromo-3-fluoro-5,8-dimethyl- benzo[c][l,8]naphthyridin-6-one (2.85 g, 31.4 %) . LCMS calculated for Ci4HnBrFN2O (M+H) ⁺ m/z = 321.0/323.0; found 321.0/323.0.

Step 5: 3-Fluoro-5, 8-dimethyl-l 0-vinyl-benzo [c] [1 ,8 ]naphthyridin-6-one

A mixture of 10-bromo-3-fluoro-5,8-dimethyl-benzo[c][l,8]naphthyridin -6-one (2.50 g, 7.78 mmol), potassium trifluoro(vinyl)borate (3.13 g , 23.35 mmol), dipotassium carbonate (3.23 g , 23.35 mmol) and palladium triphenylphosphine (1.35 g , 1.17 mmol) in THF (40 mL) and water (10 mL) was stirred at 70 °C for 12 h under nitrogen atmosphere. Upon cooling to room temperature, the mixture was diluted with water (100 mL) with stirring. The white precipitate was collected by filtration and washed with water. The crude product was directly used in the following step without further purification. LCMS calculated for C16H14FN2O (M+H) ⁺ m/z = 269.1; found 269.1.

Step 6: 3-Fluoro-5, 8-dimethyl-6-oxo-benzo[c] [1,8 ]naphthyridine-l 0-carbaldehyde

To a stirred solution of 3-fluoro-5,8-dimethyl-10-vinyl-benzo[c][l,8]naphthyridin-6- one (2.10 g , 7.83 mmol), sodium periodate (8.37 g , 39.14 mmol) and 2,6-dimethylpyridine (1.81 mL, 15.65 mmol) in 50 mL THF and 15 mL H2O was added tetraoxoosmium (0.16 g , 0.63 mmol) (4% aq. solution) dropwise and the resulting reaction mixture was kept stirred at rt for 3 h. LCMS analysis indicated the reaction was complete. The reaction mixture was diluted with water and extracted with EtOAc. The combined organics were washed with sat. NaCl and dried with anhydrous sodium sulfate, fdtered, and concentrated under reduced pressure to afford 3-fluoro-5,8-dimethyl-6-oxo-benzo[c][l,8]naphthyridine-10-ca rbaldehyde (1.81 g, 85%) as a crude product which was directly used in the following step without further purification. LCMS calculated for C15H12FN2O2 (M+H) ⁺ m/z = 271.1; found 271.1.

Step 7: 3-Fluoro-l 0-(l-hydroxyethyl)-5, 8-dimethyl-benzo[c] [1 ,8 ]naphthyridin-6-one

To a stirred solution of 3-fluoro-5,8-dimethyl-6-oxo-benzo[c][l,8]naphthyridine-10- carbaldehyde (1.80 g, 6.66 mmol) in 50 mL THF was added bromo(methyl)magnesium (6.66 mL, 19.98 mmol) dropwise at -78 °C. The resulting mixture was stirred at same temperature for 10 min, and then warmed to rt. LCMS analysis indicated the reaction was complete. The reaction was quenched with sat. NH4CI and diluted with water and extracted with EtOAc. The combined organics were washed with sat. NaCl and dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with DCM and ethyl acetate to provide the desired product 3- fluoro-10-(l-hydroxyethyl)-5,8-dimethyl-benzo[c][l,8]naphthy ridin-6-one (710 mg, 37%). LCMS calculated for Ci6Hi ₆ FN ₂ O ₂ (M+H) ⁺ m/z = 287.1; found 287.1.

Step 8: l-(3-Fluoro-5,8-dimethyl-6-oxo-benzo[c] [1 ,8]naphthyridin-10-yl)ethyl me thane sulfonate

To a stirred solution of 3-fluoro-10-(l-hydroxyethyl)-5,8-dimethyl- benzo[c][l,8]naphthyridin-6-one (70 mg , 0.24 mmol) and methanesulfonyl chloride (28 uL, 0.37 mmol) in 2 mL DCM was added N,N-diethylethanamine (136 uL, 0.98 mmol) at 0 °C. The resulting mixture was stirred at same temperature for 10 min, and then warmed to rt and stirred for 1 h. The reaction mixture was concentrated to dryness and the crude was directly used in the following step without further purification.

Step 9: 2-[l-(3-Fluoro-5,8-dimethyl-6-oxo-benzo[c][l,8]naphthyridin- 10- yl)ethylamino]benzoic acid

1 -(3 -Fluoro-5 ,8 -dimethyl-6-oxo-benzo [c] [ 1 , 8]naphthyridin- 10-yl)ethyl methanesulfonate (80 mg , 0.22 mmol) in 2 dram vial was added DMF (1 mL) and 2- aminobenzoic acid (90 mg, 0.66 mmol). The reaction mixture was stirred at 100 °C for 12 h. Upon cooling to room temperature, the mixture was diluted with water and extracted with EtOAc 3 times. The combined organics were washed with sat. NaCl, dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with DCM and ethyl acetate to provide the desired product (36 mg). LCMS calculated for C23H21FN3O3 (M+H) ⁺ m/z = 406.2; found 406.2.

Step 10: 2-[l-(3-Methoxy-5, 8-dimethyl-6-oxo-benzo[c] [1, 8 ]naphthyridin-l 0- yl)ethylamino]benzoic acid

2-[l-(3-Fluoro-5,8-dimethyl-6-oxo-benzo[c][l,8]naphthyrid in-10- yl)ethylamino] benzoic acid, (8 mg , 0.02 mmol) in 2 dram vial was added DMF (0.3 mL) and sodium methanolate (3.2 mg , 0.06 mmol). The mixture was heated to 90 °C for 2 h. Upon cooling to room temperature, the mixture was diluted with MeOH and purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product. LCMS calculated for C ₂ 4H23N ₃ NaO4 (M+Na) ⁺ m/z = 440.2; found 440.2.

Example 33: 2-[l-[3-(Dimethylamino)-5,8-dimethyl-6-oxo-benzo[c] [l,8]naphthyridin-10- yl] ethylamino] benzoic acid (1-33)

2-[l-(3-Fluoro-5,8-dimethyl-6-oxo-benzo[c][l,8]naphthyrid in-10- yl)ethylamino] benzoic acid, (6.0 mg , 0.01 mmol) in 2 dram vial was added DMF (0.3 mL) and N-methylmethanamine (29.6 uL, 0.06 mmol). The mixture was heated to 90 °C for 2 h. Upon cooling to room temperature, the mixture was diluted with MeOH and purified by prep- HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product. LCMS calculated for C ₂ 5H26N ₄ NaO3 (M+Na) ⁺ m/z = 453.2; found 453.2.

Example 34: 2-[l-[3-(2-Methoxyethoxy)-5,8-dimethyl-6-oxo-benzo[c] [l,8]naphthyridin- 10-yl]ethylamino]benzoic acid (1-34)

To a stirred solution of 2-methoxyethanol (100 mg, 1.31 mmol) in 1 mL THF was added sodium hydride (60% dispersion in mineral oil, 47.3 mg, 1.18 mmol) at 0 °C. The reaction mixture was stirred at 0 °C for Ih. And 50 μL of the above solution was added to a solution of 2-[ 1 -(3 -fluoro-5 , 8-dimethyl-6-oxo-benzo [c] [ 1 , 8]naphthyridin- 10- yl)ethylamino]benzoic acid (6.0 mg, 0.01 mmol) in DMF (0.3 mL). The mixture was heated to 90 °C for 2 h. Upon cooling to room temperature, the mixture was diluted with MeOH and purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product. LCMS calculated for C26H2?N3NaO5(M+Na) ⁺ m/z = 484.2; found 484.2.

Example 35: 2-((l-(7-Chloro-3,4-dimethyl-5-oxo-4,5-dihydro-3H-pyrazolo [3,4- c]isoquinolin-9-yl)ethyl)amino)benzoic acid (1-35)

The titled compound was prepared using similar procedures as described for Example 16 with 3 -3 -bromo-5-chloro-2 -iodobenzoic acid replacing 3 -bromo -2 -iodo -5 -methylbenzoic acid in Step 1. The resulting mixture was purified by prep-HPLC (column: Sunfire prep Cl 8 column, 30* 150 mm, 5 μm; mobile phase A: water (0. 1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C21H20CIN4O3 (M+H) ⁺ m/z = 411.1; found 411.1.

Example 36: 2-((l-(8-Methyl-6-oxo-4,5-dihydro-3H,6H-2,2a,5a-triazaaceant hrylen-10- yl)ethyl)amino)benzoic acid (1-36)

Step 1 : 3-Bromo-2-iodo-5-methylbenzoic acid

2-Amino-3-bromo-5-methyl-benzoic acid (25 g, 108.6 mmol) was suspended in 120 mL water with stirring. The mixture was cooled in an ice bath and 100 mL concentrated sulfuric acid was added slowly. A solution of sodium nitrite (13.50 g, 195.60 mmol) in 100 mL water was added dropwise over 0.5 hour. The reaction mixture was stirred for an additional 0.25 hour at 0-5°C. The mixture was poured into a well -stirred solution of potassium iodide (27.06 g, 163.00 mmol) in 100 mL water. The resulting solid was collected by fdtration and stirred in 200 mL ethyl acetate. A solution of 200 mL 20% sodium hydrogen sulfite was added slowly. The organic layer was separated, washed with 100 mL 20% sodium hydrogen sulfite, 50 mL saturated sodium chloride, dried (magnesium sulfate) and concentrated under reduced pressure. The residue was recrystallized from ether/hexane to provide 3-bromo-2-iodo-5-methyl-benzoic acid (22.00 g, 59.4%). LCMS calculated for C ₈ H ₇ BrIO ₂ (M+H) ⁺ m/z = 340.9; found 340.9.

Step 2: (3-Bromo-2-iodo-5-methylphenyl)(6, 7-dihydropyrazolo[l,5-a]pyrimidin-4(5H)- yl)methanone

To a mixture of 3 -bromo-2-iodo-5 -methylbenzoic acid (664 mg, 1.95 mmol) in DMF (5 mL) was added HATU (741 mg, 1.95 mmol), 4,5,6,7-tetrahydropyrazolo[l,5-a]pyrimidine (200 mg, 1.62 mmol) and DIPEA (0.85 mL, 4.87 mmol). The reaction mixture was stirred at r.t. (room temperature) for 4 h. After completion, the reaction was quenched with water. The mixture was then extracted with ethyl acetate (2 x 300 mL). The combined organics were dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure, which was further purified by silica gel column chromatography, eluted with DCM and ethyl acetate to provide the desired product as white solid (520 mg, 72%). LCMS calculated for Ci ₄ Hi ₄ BrIN ₃ O (M+H) ⁺ m/z = 445.9; found 445.9.

Step 3: 10-Bromo-8-methyl-4, 5-dihydro-3H, 6H-2, 2a, 5a-triazaaceanthrylen-6-one

A mixture of (3-bromo-2-iodo-5-methylphenyl)(6,7-dihydropyrazolo[l,5- a]pyrimidin-4(5H)-yl)methanone (500 mg, 1.12 mmol), Pd(PPh ₃ ) ₄ (647 mg, 0.56 mmol), KOAc (660 mg, 6.73 mmol) in DMAc (5 mL) was heated at 120 °C for 16 h under nitrogen atmosphere. Upon cooling to room temperature, the mixture was diluted with water and extracted with EtOAc. The combined organics were washed with sat. NaCl and dried with anhydrous sodium sulfate, fdtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with DCM and ethyl acetate to provide the desired product as a white solid (210 mg, 59%). LCMS calculated for CuHnBrNsO (M+H) ⁺ m/z = 318.0; found 318.0.

Step 4: 10-Acetyl-8-methyl-4, 5-dihydro-3H, 6H-2, 2a, 5a-triazaaceanthrylen-6-one

A mixture of 10-bromo-8-methyl -4, 5 -dihydro-3 H,6H-2, 2a, 5a-triazaaceanthrylen-6- one (210 mg, 0.66 mmol), PdC12(PPh3)2 (92 mg, 0.13 mmol), and tributyl(l- ethoxyvinyl)stannane (357 mg, 0.99 mmol) in dioxane (5 mL) was heated at 100 °C for 2 h under nitrogen atmosphere. Upon cooling to room temperature, the mixture was added 2 N HC1 and stirred for 30 min. Then 200 mg CsF was added and stirred for another 30 min before the mixture was diluted with water and extracted with EtOAc. The combined organics were washed with sat. NaCl and dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with DCM and ethyl acetate to provide the desired product as a white solid (150 mg, 81%). LCMS calculated for C16H16N3O2 (M+H) ⁺ m/z = 282.1; found 282.1.

Step 5: 10-(l-Hydroxyethyl)-8-methyl-4, 5-dihydro-3H, 6H-2, 2a, 5a-triazaaceanthrylen-6-one

To a mixture of 10-acetyl-8-methyl-4,5-dihydro-3H,6H-2,2a,5a-triazaaceanthry len-6- one (150 mg, 0.53 mmol) in THF (2 mL) was added LAH (0.8 mL, 0.8 mmol, 1 M solution in THF) at -78 °C. The resulting mixture was stirred at same temperature for 10 min, and then warmed to -40 °C before quenched with sat. NH4CI. The mixture was diluted with water and extracted with EtOAc. The combined organics were washed with sat. NaCl and dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with DCM and ethyl acetate to provide the desired product as a white solid (140 mg, 92%). LCMS calculated for C16H18N3O2 (M+H) ⁺ m/z = 284. 1; found 284. 1.

Step 6: 10-(l-Bromoethyl)-8-methyl-4, 5-dihydro-3H, 6H-2, 2a, 5a-triazaaceanthrylen-6-one

To a mixture of 10-(l-hydroxyethyl)-8-methyl-4,5-dihydro-3H,6H-2,2a,5a- triazaaceanthrylen-6-one (20 mg, 0.07 mmol) in DCM (1 mL) was added PBrs (38 mg, 0.14 mmol). The resulting mixture was stirred at rt for 2 h. The mixture was then quenched with sat. NaHCO, and extracted with DCM. The combined organics were washed with sat. NaCl, dried with anhydrous sodium sulfate, fdtered, and concentrated under reduced pressure. The residue was used directly in next step without further purification.

Step 7: 2-((l-(8-Methyl-6-oxo-4, 5-dihydro-3H, 6H-2, 2a, 5a-triazaaceanthrylen-l 0- yl)ethyl)amino)benzoic acid

To 10-(l-Bromoethyl)-8-methyl-4,5-dihydro-3H,6H-2,2a,5a-triazaa ceanthrylen-6-one (20 mg, 0.06 mmol) in 2 dram vial was added DMF (0.3 mL) and 2 -aminobenzoic acid (20 mg, 0. 14 mmol). The mixture was heated to 90 °C for 2 h. Upon cooling to room temperature, the mixture was diluted with MeOH and purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C23H23N4O3 (M+H) ⁺ m/z = 403.2; found 403.2.

Example 37: 6-Methyl-3-((l-(8-methyl-6-oxo-4,5-dihydro-3H,6H-2,2a,5a- triazaaceanthrylen-10-yl)ethyl)amino)picolinic acid (1-37) The titled compound was prepared using similar procedures as described for Example 36 with 3 -amino -6 -methylpicolinic acid replacing 2 -aminobenzoic acid in Step 7. The resulting mixture was purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5 μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C23H24N5O3 (M+H) ⁺ m/z = 418.2; found 418.2.

Example 38: 2-((l-(3,8-Dimethyl-6-oxo-4,5-dihydro-3H,6H-2,2a,5a-triazaac eanthrylen- 10-yl)ethyl)amino)benzoic acid (1-38)

The titled compound was prepared using similar procedures as described for Example 36 with 7-methyl-4,5,6,7-tetrahydropyrazolo[l,5-a]pyrimidine replacing 4, 5,6,7- tctrahydropyrazolo| 1,5 -a] pyrimidine in Step 2. The resulting mixture was purified by prep- HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5 μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C24H25N4O3 (M+H) ⁺ m/z = 417.2; found 417.2.

Example 39: 6-Chloro-3-((l-(3,8-dimethyl-6-oxo-4,5-dihydro-3H,6H-2,2a,5a - triazaaceanthrylen-10-yl)ethyl)amino)picolinic acid (1-39)

The titled compound was prepared using similar procedures as described for Example 36 with 7-methyl-4,5,6,7-tetrahydropyrazolo[l,5-a]pyrimidine replacing 4, 5,6,7- tctrahydropyrazolo| 1,5 -a] pyrimidine in Step 2, and 3-amino-6-chloropicolinic acid replacing 2 -aminobenzoic acid in Step 7. The resulting mixture was purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5 μm; mobile phase A: water (0. 1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C23H23CIN5O3 (M+H) ⁺ m/z = 452. 1; found 452. 1.

Example 40. 2-Ethyl-3,6-dimethyl-8-((6-methyl- [2,4'-bipyridin] -3-yl)amino)-8,9- dihydrobenzo[de]pyrazolo[4,5,l-ij][l,7]naphthyridin-4(3H)-on e

Step 1. 2-Bromo-3-iodo-5-methylbenzoic acid

In a 1 L three necked flask fitted with a dropping funnel and thermometer, were charged 2-amino-3-iodo-5 -methylbenzoic acid (17.8 g, 64.2 mmol) and acetonitrile (160 mb). After cooling to 0 °C, HBr (47%, 160 mL) was added drop wise over 10 min. To the resulting solution a solution of NaNCh (4.88 g, 70.7 mmol) in water (20.0 mL) was added drop wise over 1 h. After addition, the reaction mixture was stirred at 0 °C for 30 min, and copper(I) bromide (11.06 g, 77.1 mmol) was added portion wise over 30 min. The resulting mixture was then heated at 70° C in an oil bath for 1 h. After cooling to 0 °C, 700 mL of water was added and the precipitate was filtered, washed with cold water and dried under vacuum to give the title compound as a white solid. The crude product was used in the next step without further purification.

Step 2. 2-Bromo-3-iodo-N,N, 5 -trimethylbenzamide

To a stirred solution of 2-bromo-3-iodo-5 -methylbenzoic acid (5.0 g, 14.7 mmol) in DMF (50 mL) was added HATU (7.2 g, 19.1 mmol), dimethylamine (2M THF, 8 mL, 16 mmol) and N-ethyl-N-isopropyl-propan-2-amine (3.33 mL, 19.1 mmol). The resulting mixture was kept stirring overnight before quenched with ice water. The mixture was extracted with EtOAc. The combined organics were washed with sat. NaCl, dried with anhydrous sodium sulfate, fdtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with hexanes and ethyl acetate to provide the desired product as a brown solid (4.8 g, 90%). LCMS calculated for CioHi ₂ BrINO (M+H) ⁺ m/z = 368.0; found 368.0.

Step 3. 2-Bromo-N,N,5-trimethyl-3-vinylbenzamide

The mixture of 2-bromo-3-iodo-N,N, 5 -trimethylbenzamide (3.0 g, 8.8 mmol), PdCl ₂ (PPh ₃ ) ₂ (572 mg, 0.82 mmol), and tributyl(vinyl)stannane (2.84 g, 8.97 mmol) in dioxane (50 mL) was heated at 100 °C for 2 h under nitrogen atmosphere. Upon cooling to room temperature, the mixture was diluted with water and extracted with EtOAc. The combined organics were washed with sat. NaCl, dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with DCM and ethyl acetate to provide the desired product as a brown solid (2.0 g, 85%). LCMS calculated for Ci ₂ FUBrNO (M+H) ⁺ m/z = 268.0; found 268.1.

Step 4. 2-Bromo-N,N,5-trimethyl-3-(oxiran-2-yl)benzamide

To a mixture of 2-bromo-N,N,5-trimethyl-3-vinylbenzamide (4.3 g, 16.1 mmol) in DCM (500 mL) was added mCPBA (4.7 g, 19.2 mmol) at 0 °C in three portions over Ih. The reaction mixture was then warmed to rt and stirred for 2 h. After completion, the reaction was diluted with ice water and washed with sat NaHCO ₃ and sat. NaCl, dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure to give the title compound as a light-yellow oil. The crude product was used in the next step without further purification. Step 5. 2-Bromo-3-(l-hydroxy-2-(4-nitro-lH-pyrazol-l-yl)ethyl)-N,N, 5-trimethylbenzamide

O YA >T Br

HCT A

,N, p O ₂ N

To a mixture of 2-bromo-N,N,5-trimethyl-3-(oxiran-2-yl)benzamide (2.0 g, 7.1 mmol) in PhCR (100 mL) was added 4-nitro-lH-pyrazole (1.6 g, 14.1 mmol) and CS2CO3 (1.1 g, 3.5 mmol) at rt. The resulting mixture was stirred at 110 °C for 6h. Upon cooling to room temperature, the reaction was diluted with water and extracted with DCM/IPA 3: 1. The combined organics were washed with sat. NaCl, dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with DCM and MeOH to provide the desired product as a brown oil (1.3 g, 45%). LCMS calculated for CisHisBrN-tCL (M+H) ⁺ m/z = 397.0; found 397.1.

Step 6. 2-Bromo-N,N,5-trimethyl-3-(2-(4-nitro-lH-pyrazol-l-yl)-l-((2 -

(trimethylsilyl)ethoxy)methoxy)ethyl)benzamide

To a mixture of 2-bromo-3-(l-hydroxy-2-(4-nitro-lH-pyrazol-l-yl)ethyl)-N,N,5 - trimethylbenzamide (830 mg, 2.1 mmol) in DMF (8 mL) was added NaH (100 mg, 2.5 mmol) at 0 °C under nitrogen atmosphere. The resulting mixture was stirred at same temperature for 30 min and then SEMC1 (418 mg, 2.5 mmol) was added. The reaction mixture was then slowly warmed to rt and stirred for 1 h. After completion, the reaction was carefully quenched with water at 0 °C while vigorously stirred. The mixture was then extracted with ethyl acetate (2 x 50 mL). The combined organics were dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with hexanes and ethyl acetate to provide the desired product, which was further purified by silica gel column chromatography, eluted with hexanes and ethyl acetate to provide the desired product as a white solid (883 mg, 80%). LCMS calculated for C ₂ iH ₃₂ BrN ₄ O ₅ Si (M+H) ⁺ m/z = 527.1; found 527.1.

Step 7. N,N,8-Trimethyl-l-nitro-6-((2-(trimethylsilyl)ethoxy)methoxy )-5, 6- dihydropyrazolo[5,l-a]isoquinoline-10-carboxamide

The mixture of 2-bromo-N,N,5-trimethyl-3-(2-(4-nitro-lH-pyrazol-l-yl)-l-((2 - (trimethylsilyl)ethoxy)methoxy)ethyl)benzamide (883 mg, 1.68 mmol), Pd(PPh ₃ ) ₄ (576 mg, 0.5 mmol), KOAc (1.0 g, 10.1 mmol) in DMAc (10 mL) was heated at 120 °C for 2 h under nitrogen atmosphere. Upon cooling to room temperature, the mixture was diluted with water and extracted with EtOAc. The combined organics were washed with sat. NaCl and dried with anhydrous sodium sulfate, fdtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with hexanes and ethyl acetate to provide the desired product as a white solid (446 mg, 65%). LCMS calculated for C ₂ iH ₃ iN ₄ O ₅ Si (M+H) ⁺ m/z = 447.2; found 447.2.

Step 8: l-Amino-N,N,8-trimethyl-6-((2-(trimethylsilyl)ethoxy)methoxy )-5,6- dihydropyrazolo[5, 1 -a]isoquinoline-l 0-ccirb oxamide

The mixture of N,N,8-trimethyl-l-nitro-6-((2-(trimethylsilyl)ethoxy)methoxy )-5,6- dihydropyrazolo[5,l-a]isoquinoline-10-carboxamide (446 mg, 1.0 mmol), and Fe (223 mg, 4 mmol), in MeOH (3 mL), THF (3 mL) and sat. NH ₄ C1 (1.5 mL) was heated at 60 °C for 2 h under nitrogen atmosphere. Upon cooling to room temperature, the mixture was diluted with water and extracted with EtOAc. The combined organics were washed with sat. NaCl and dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with DCM and MeOH to provide the desired product as a brown oil (183 mg, 44%). LCMS calculated for C ₂ iH ₃₃ N ₄ O ₃ Si (M+H) ⁺ m/z = 417.2; found 417.2. Step 9: 6-Methyl-8-((2-(trimethylsilyl)ethoxy)methoxy)-8,9-dihydrobe nzo[de]pyrazolo[4,5,l- ijJ' [1, 7]naphthyridin-4(3H)-one

The mixture of l-amino-N,N,8-trimethyl-6-((2-(trimethylsilyl)ethoxy)methoxy )-5,6- dihydropyrazolo[5,l-a]isoquinoline-10-carboxamide (183 mg, 0.44 mmol) in THF (3 mL) was added LDA (2M in THF, 0.3 mL, 0.6 mmol) at -78 °C under nitrogen atmosphere. After stirring for 10 min at -78 °C, the mixture was diluted sat. NH4CI and extracted with EtOAc. The combined organics were washed with sat. NaCl and dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with DCM and MeOH to provide the desired product as a brown oil (127 mg, 78%). LCMS calculated for CigfteNsChSi (M+H) ⁺ m/z = 372.2; found 372.2.

Step 10: 3, 6-Dimethyl-8-((2-(trimethylsilyl)ethoxy)methoxy)-8, 9- dihydrobenzo[de]pyrazolo[4,5,l-ij] [1, 7]naphthyridin-4(3H)-one

To a mixture of 6-methyl-8-((2-(trimethylsilyl)ethoxy)methoxy)-8,9- dihydrobenzo[de]pyrazolo[4,5,l-ij][l,7]naphthyridin-4(3H)-on e (127 mg, 0.34 mmol) in DMF (2 mL) was added NaH (17 mg, 0.44 mmol) at 0 °C under nitrogen atmosphere. The resulting mixture was stirred at same temperature for 30 min and then Mel (62 mg, 0.44 mmol) was added. The reaction mixture was then slowly warmed to rt and stirred for 1 h. After completion, the reaction was carefully quenched with water at 0 °C while vigorously stirred. The mixture was then extracted with ethyl acetate (2 x 5 mL). The combined organics were dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with hexanes and ethyl acetate to provide the desired product, which was further purified by silica gel column chromatography, eluted with DCM and MeOH to provide the desired product as a white solid (102 mg, 80%). LCMS calculated for C2oH2gN303Si (M+H) ⁺ m/z = 386.2; found 386.2. Step 11. 2-Bromo-3, 6-dimethyl-8-((2-(trimethylsilyl)ethoxy)methoxy)-8,9- dihydrobenzo[de]pyrazolo[4,5,l-ij] [1, 7]naphthyridin-4(3H)-one

To a mixture of 2-bromo-3,6-dimethyl-8-((2-(trimethylsilyl)ethoxy)methoxy)-8 ,9- dihydrobenzo[de]pyrazolo[4,5,l-ij][l,7]naphthyridin-4(3H)-on e (102 mg, 0.22 mmol) in DCM (2 mL) was added NBS (39 mg, 0.22 mmol) at 0 °C under nitrogen atmosphere. The reaction mixture was then slowly warmed to rt and stirred for 30 min. After completion, the reaction was diluted with sodium thiosulfate solution while stirred. The mixture was then extracted with ethyl acetate (2 x 5 mL). The combined organics were dried with anhydrous sodium sulfate, fdtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with hexanes and ethyl acetate to provide the desired product, which was further purified by silica gel column chromatography, eluted with DCM and EtOAc to provide the desired product as a light-yellow solid (76 mg, 75%). LCMS calculated for C2oH2?BrN303Si (M+H) ⁺ m/z = 464.1; found 464.1.

Step 12. 3,6-Dimethyl-8-((2-(trimethylsilyl)ethoxy)methoxy)-2-vinyl-8 ,9- dihydrobenzo[de]pyrazolo[4,5,l-ij] [1, 7]naphthyridin-4(3H)-one

The mixture of 2-bromo-3,6-dimethyl-8-((2-(trimethylsilyl)ethoxy)methoxy)-8 ,9- dihydrobenzo[de]pyrazolo[4,5,l-ij][l,7]naphthyridin-4(3H)-on e (76 mg, 0.16 mmol), PdCl ₂ (PPh ₃ )2 (14 mg, 0.02 mmol), and tributyl(vinyl)stannane (63 mg, 0.2 mmol) in dioxane (2 mL) was heated at 100 °C for 2 h under nitrogen atmosphere. Upon cooling to room temperature, the mixture was diluted with water and extracted with EtOAc. The combined organics were washed with sat. NaCl, dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with DCM and ethyl acetate to provide the desired product as a brown solid (59 mg, 90%). LCMS calculated for C22H3oN303Si (M+H) ⁺ m/z = 412.2; found 412.2. Step 13. 2-Ethyl-3, 6-dimethyl-8-((2-(trimethylsilyl)ethoxy)methoxy)-8,9- dihydrobenzo[de]pyrazolo[4,5,l-ij] [1, 7]naphthyridin-4(3H)-one

In a 2 mL drum vial, 3,6-dimethyl-8-((2-(trimethylsilyl)ethoxy)methoxy)-2-vinyl-8 ,9- dihydrobenzo[de]pyrazolo[4,5,l-ij][l,7]naphthyridin-4(3H)-on e (59 mg, 0.14 mmol) and Pd/C (10 wt% on active carbon, 10 mg) was dissolved in MeOH (5 mL). The vial was then charged with latm H2 and stirred for 8h. The reaction mixture was then filtrate through a short pad of celite and solvent was removed under vacuum to afford the crude product which was used in next step without further purification.

Step 14. 2-Ethyl-8-hydroxy-3, 6-dimethyl-8, 9-dihydrobenzo[de]pyrazolo[4, 5,1- ijJ' [1, 7]naphthyridin-4(3H)-one

In a 2 mL drum vial, 2-ethyl-3,6-dimethyl-8-((2-(trimethylsilyl)ethoxy)methoxy)-8 ,9- dihydrobenzo[de]pyrazolo[4,5,l-ij][l,7]naphthyridin-4(3H)-on e (50 mg, 0.12 mmol) was dissolved in TFA (1 mL). The reaction mixture was stirred for 1 h, and solvent was removed under vacuum. The crude was diluted with sat. NaHCCh and extracted with DCM/IPA. The combined organics were washed with sat. NaCl, dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with DCM and MeOH to provide the desired product as an oil (27 mg, 80%). LCMS calculated for C16H18N3O2 (M+H) ⁺ m/z = 284.1; found 284.1.

Step 15. 8-Bromo-2-ethyl-3, 6-dimethyl-8, 9-dihydrobenzo [de ]pyrazolo[4, 5,1- ijJ' [1, 7]naphthyridin-4(3H)-one To a mixture of 2-ethyl-8-hydroxy-3,6-dimethyl-8,9- dihydrobenzo[de]pyrazolo[4,5,l-ij][l,7]naphthyridin-4(3H)-on e (27 mg, 0.09 mmol) in DCM (2 mL) was added PBr, (20 uL, 0.2 mmol) at 0 °C. The resulting mixture was warmed to rt and stirred for 2 h. The mixture was then quenched with sat. NaHCCh and extracted with DCM (2x). The combined organics were washed with sat. NaCl, and dried with anhydrous sodium sulfate, fdtered, and concentrated under reduced pressure. The residue was used directly in next step without further purification.

Step 16. 8-((2-Bromo-6-methylpyridin-3-yl)amino)-2-ethyl-3, 6-dimethyl-8, 9- dihydrobenzo[de]pyrazolo[4,5,l-ij] [1, 7]naphthyridin-4(3H)-one

To the solution of 8-bromo-2-ethyl-3,6-dimethyl-8,9- dihydrobenzo[de]pyrazolo[4,5,l-ij][l,7]naphthyridin-4(3H)-on e (24 mg, 0.07 mmol) in DMF (0.3 mL) was added 2-bromo-6-methylpyridin-3-amine (40 mg, 0.2 mmol). The mixture was heated to 80 °C for 2 h. Upon cooling to room temperature, the mixture was diluted with water and extracted with ethyl acetate (3X). The combined organics were washed with water, sat. NaCl, and dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with DCM and MeOH to provide the desired product as a light yellow solid (13 mg, 40%). LCMS calculated for C22H23BrN5O (M+H) ⁺ m/z = 452.1; found 452.1.

Step 17. 2-Ethyl-3, 6-dimethyl-8-(( 6-methyl-[2, 4 '-bipyridin] -3-yl)amino)-8, 9- dihydrobenzo[de]pyrazolo[4,5,l-ij] [1, 7]naphthyridin-4(3H)-one

The mixture of 8-((2-bromo-6-methylpyridin-3-yl)amino)-2-ethyl-3,6-dimethyl -8,9- dihydrobenzo[de]pyrazolo[4,5,l-ij][l,7]naphthyridin-4(3H)-on e (10 mg, 0.02 mmol), Pd(PPh3)4 (3 mg, 0.003 mmol), and pyridin-4-ylboronic acid (4 mg, 0.03 mmol) in dioxane (0.5 mL) and water (0.1 mL) was heated at 80°C for 10 min under nitrogen atmosphere. Upon cooling to room temperature, the mixture was diluted with MeOH and purified by prep- HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C27H27N6O (M+H) ⁺ m/z = 451.2; found 451.2. Example 41: 9-(l-((6-chloro-l'-methyl-6'-oxo-l',6'-dihydro-[2,3'-bipyrid in]-3- yl)amino)ethyl)-3-ethyl-4,7-dimethyl-3,4-dihydro-5/f-pyrazol o[3,4-c]isoquinolin-5-one

Step 1. 9-(l-((2-Bromo-6-chloropyridin-3-yl)amino)ethyl)-3-ethyl-4, 7 -dimethyl- 3, 4-dihydro- 5H-pyrazolo[3, 4-c]isoquinolin-5-one

The titled compound was prepared using similar procedures as described for Example 16 with 2-bromo-6-chloropyridin-3-amine replacing 2-aminobenzoic acid in Step 7 and 1- ethyl-lH-pyrazol-5-amine replacing 1 -methyl -IH-pyrazol -5 -amine in step 1. Crude product was purified with silica gel chromatography, eluting with 0% to 100% EtOAc in DCM to give the desired product as a beige solid. LCMS calculated for C2iH22BrClN5O (M+H) ⁺ m/z = 474.1; found 474.

Step 2. 9-(l-((6-Chloro-l '-methyl-6'-oxo-l ',6'-dihydro-[2,3'-bipyridin]-3-yl)amino)ethyl)-3- ethyl-4, 7-dimethyl-3, 4-dihydro-5H-pyrazolo[3, 4-c]isoquinolin-5-one

The mixture of 9-[l-[(2-bromo-6-chloro-3-pyridyl)amino]ethyl]-3-ethyl-4,7- dimethyl-pyrazolo[3,4-c]isoquinolin-5-one (10.60 mg, 0.02 mmol), 1 -methyl-5-(4, 4,5,5- tetramethyl-l,3,2-dioxaborolan-2-yl)pyridin-2-one (7.87 mg, 0.03 mmol), potassium carbonate (9.26 mg, 0.07 mmol) and tetrakis(triphenylphosphine)palladium(0) (2.6 mg, 0.002 mmol) in a 5 to 1 mixture of toluene and water (0.2 mL) was heated at 100 °C for 1 h under nitrogen atmosphere. Upon cooling to room temperature, the mixture was concentrated under reduced pressure. The residue was diluted with methanol and purified by prep-HPLC (column: Sunfire prep 15 C18 column, 30* 150 mm, 5μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C27H28CIN6O2 (M+H) ⁺ m/z = 503.2; found: 503.2; The following compounds in Table 4 were prepared similarly as described for Example 41.

Table 4.

Example 52. 3-Ethyl-4,7-dimethyl-9-(l-((6-methyl-2-(l-methyl-lH-pyrazol- 4-yl)pyridin-

3-yl)amino)ethyl)-3,4-dihydro-5H-pyrazolo[3,4-c]isoquinol in-5-one

Step 1. 9-(l-((2-Bromo-6-methylpyridin-3-yl)amino)ethyl)-3-ethyl-4, 7-dimethyl-3, 4-dihydro- 5H-pyrazolo[3, 4-c]isoquinolin-5-one

The titled compound was prepared using similar procedures as described for Example 16 with l-ethyl-lH-pyrazol-5-amine replacing l-methyl-lH-pyrazol-5-amine in Step 1 and 2- bromo-6-methylpyridin-3-amine replacing 2-aminobenzoic acid in Step 7. The resulting mixture was diluted with water and extracted with EtOAc. The combined organics were washed with sat. NaCl, dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with DCM and ethyl acetate to provide the desired product as a white solid (248 mg, 83%). LCMS calculated for C22H25BrN5O (M+H) ⁺ m/z = 454.1; found 454.1.

Step 2: 3-Ethyl-4, 7 -dimethyl-9-(l-((6-methyl-2-(l -methyl- lH-pyrazol-4-yl)pyridin-3- yl)amino)ethyl)-3, 4-dihydro-5H-pyrazolo[3,4-c]isoquinolin-5-one

The mixture of 9-(l-((2-bromo-6-methylpyridin-3-yl)amino)ethyl)-3-ethyl-4,7 - dimethyl-3,4-dihydro-5H-pyrazolo[3,4-c]isoquinolin-5-one (10 mg, 0.02mmol), l-methyl-4- (4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl)-lH-pyrazole (9 mg, 0.04mml), CS2CO3 (14 mg, 0.04 mmol) and l,l'-Bis(diphenylphosphino)ferrocene dichloropalladium (II) (1.6 mg, 0.002mmol) in dioxane (0.5 mb) and water (0.1 mb) was heated at 80 °C for 1 h under nitrogen atmosphere. The mixture was diluted with MeOH and purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C26H30N7O (M+H) ⁺ m/z = 456.3; found 456.3. The following compounds in Table 5 were prepared similarly as described for

Example 52.

Table 5.

Example 56: 3-ethyl-9-(l-((2-(l-(2-hydroxyethyl)-lH-pyrazol-4-yl)phenyl) amino)ethyl)-

4,7-dimethyl-3,4-dihydro-5/f-pyrazolo[3,4-c]isoquinolin-5 -one

Step 1. 3-Ethyl-9-(l-((2-iodophenyl)amino)ethyl)-4, 7-dimethyl-3,4-dihydro-5H-pyrazolo[3,4- c ] isoquinolin-5-one

The titled compound was prepared using similar procedures as described for Example 16 with 2-iodoaniline replacing 2-aminobenzoic acid in Step 7 and l-ethyl-lH-pyrazol-5- amine replacing l-methyl-lH-pyrazol-5-amine in Step 1. The crude mixture was purified with silica gel chromatography, eluting with 0% to 100% EtOAc in DCM to give the desired product as a white solid. LCMS calculated for C22EI24IN4O (M+H) ⁺ m/z = 487. 1; found 487. 1.

Step 2. 3-Ethyl-9-(l-((2-(l-(2-hydroxyethyl)-lH-pyrazol-4-yl)phenyl) amino)ethyl)-4, 7- dimethyl-3, 4-dihydro-5H-pyrazolo[3, 4-c]isoquinolin-5-one

The mixture of 3-ethyl-9-(l-((2-iodophenyl)amino)ethyl)-4,7-dimethyl-3,4-di hydro- 5H-pyrazolo[3,4-c]isoquinolin-5-one (10.0 mg, 0.02 mmol), Pd(dppf)Ch • DCM (2.0 mg, 2 μmol), (l-(2-hydroxyethyl)-lH-pyrazol-4-yl)boronic acid (4.4 mg, 0.03 mmol) and cesium carbonate (22.7 mg, 0.07 mmol) in a 5 to 1 mixture of toluene and water (0.2 mb) was heated at 100 °C for 1 h under nitrogen atmosphere. Upon cooling to room temperature, the mixture was concentrated under reduced pressure. The residue was purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5μm; mobile phase A: water (0. 1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C27H ₃ IN ₆ O ₂ (M+H) ⁺ m/z = 471.2; found: 471.2.

Example 57. 9-(l-((2-(4-Acetylpiperazin-l-yl)phenyl)amino)ethyl)-3-ethyl -4,7-dimethyl-

3,4-dihydro-5/7-pyrazolo[3,4-c]isoquinolin-5-one

Step 1. 9-(l-((2-Bromophenyl)amino)ethyl)-3-ethyl-4, 7-dimethyl-3,4-dihydro-5H- pyrazolo[3, 4-c]isoquinolin-5-one

The titled was prepared using similar procedures as described for Example 16 with 2- bromoaniline replacing 2-aminobenzoic acid in Step 7 and 1 -ethyl- lH-pyrazol-5 -amine replacing l-methyl-lH-pyrazol-5-amine in step 1. The crude mixture was purified with silica gel chromatography, eluting with 0% to 100% EtOAc in DCM to give the desired product as a white solid. LCMS calculated for C22H24BrN4O (M+H) ⁺ m/z = 439. 1; found 439. 1;

Step 2: 9-(l-((2-(4-Acetylpiperazin-l-yl)phenyl)amino)ethyl)-3-ethyl -4, 7-dimethyl-3,4- dihydro-5H-pyrazolo[3, 4-c]isoquinolin-5-one

The mixture of 9-[l-(2-bromoanilino)ethyl]-3-ethyl-4,7-dimethyl-pyrazolo[3, 4- c]isoquinolin-5-one ( 10.20 mg , 0.02 mmol), 1 -piperazin- 1-ylethanone (4.5 mg, 0.03 mmol), [2-(2-aminophenyl)phenyl]-chloro-palladium;dicyclohexyl-[2-( 2,6- diisopropoxyphenyl)phenyl]phosphane (3.61 mg , 0.005 mmol) and potassium carbonate (22.7 mg, 0.07 mmol) in anhydrous 1,4-dioxane (0.2 mb) was heated at 100 °C for overnight under nitrogen. Upon cooling to room temperature, the mixture was concentrated under reduced pressure. The residue was diluted with methanol and purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C ₂ 8H35N ₆ O ₂ (M+H) ⁺ m/z = 487.3; found: 487.3.

Example 58. 9-(l-((2-(5,6-Dihydro-[l,2,4]triazolo[4,3-a]pyrazin-7(8H)- yl)phenyl)amino)ethyl)-3-ethyl-4,7-dimethyl-3,4-dihydro-5H-p yrazolo[3,4-c]isoquinolin- 5-one The titled compound was prepared using similar procedures as described for Example 57 with 5,6,7,8-tetrahydro-[l,2,4]triazolo[4,3-a]pyrazine replacing 1 -piperazin- 1-ylethanone in Step 2. The resulting mixture was purified by prep-HPLC (column: Sunfire prep Cl 8 column, 30* 150 mm, 5 μm; mobile phase A: water (0. 1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C27H31N8O (M+H) ⁺ m/z = 483.2; found 483.2.

Example 59. 3-(6-Chloro-3-((l-(3-ethyl-4,7-dimethyl-5-oxo-4,5-dihydro-3H -pyrazolo [3,4- c]isoquinolin-9-yl)ethyl)amino)pyridin-2-yl)-l,2,4-oxadiazol -5(4H)-one

Step 1. 3-Amino-6-chloro-N-hydroxypicolinimidamide

The mixture of 3-amino-6-chloropicolinonitrile (5 g, 32.5 mmol) and 50% aqueous hydroxylamine (2 mb, 32.5 mmol) in MeOH (100 mL) was stirred at rt. for 1 h. Then the mixture was poured into water (500 mL). The resulting solid was collected via filtration, washed with water (100 mL), and dried to afford the desired product as a brown solid (5.1 g, 84% yield). LCMS calculated for C6H8CIN4O (M+H) ⁺ m/z = 187.0; found 187.0.

Step 2. 3-(3-Amino-6-chloropyridin-2-yl)-l,2, 4-oxadiazol-5 ( 4H)-one To a solution of 3-amino-6-chloro-N-hydroxypicolinimidamide (5.1 g, 27.3 mmol) in THF (100 mL) was added l,l'-Carbonyldiimidazole (4.87 g, 30 mmol) and 1,8- Diazabicyclo[5.4.0]undec-7-ene (16.6 mL, 109 mmol) at 0 °C. After stirring for 4 h at rt., the mixture was diluted with 500 mL of water. The aqueous phase was washed with 50mL ethyl acetate 3 times, then adjusted pH to 7 with 3N HC1. The resulting solid was collected via filtration, washed with water (100 mL), and dried to afford the desired product as a brown solid (4.9 g, 84% yield). LCMS calculated for C7H6CIN4O2 (M+H) ⁺ m/z = 213.0; found 213.0.

Step 3: 3-(6-Chloro-3-((l-(3-ethyl-4, 7-dimethyl-5-oxo-4,5-dihydro-3H-pyrazolo[3,4- c]isoquinolin-9-yl)ethyl)amino)pyridin-2-yl)-l,2,4-oxadiazol -5(4H)-one

The titled compound was prepared using similar procedures as described for Example 16 with l-ethyl-lH-pyrazol-5-amine replacing l-methyl-lH-pyrazol-5-amine in Step 1 and 3- (3-amino-6-chloropyridin-2-yl)-l,2,4-oxadiazol-5(4H)-one replacing 2 -aminobenzoic acid in Step 7. The resulting mixture was purified by prep-HPLC (column: Sunfire prep Cl 8 column, 30* 150 mm, 5 μm; mobile phase A: water (0. 1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C23H23CIN7O3 (M+H) ⁺ m/z = 480.2; found 480.2.

Example 60. 3-(6-Chloro-3-((l-(4-(2,2-difluoroethyl)-3-ethyl-7-methyl-5- oxo-4,5- dihydro-3H-pyrazolo[3,4-c]isoquinolin-9-yl)ethyl)amino)pyrid in-2-yl)-l,2,4-oxadiazol- 5(4H)-one

The titled compound was prepared using similar procedures as described for Example 16 with l-ethyl-lH-pyrazol-5-amine replacing l-methyl-lH-pyrazol-5-amine in Step 1, 1,1- difluoro-2 -iodoethane replacing iodomethane in Step 2 and 3-(3-amino-6-chloropyridin-2-yl)- l,2,4-oxadiazol-5(4H)-one replacing 2-aminobenzoic acid in Step 7. The resulting mixture was purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5 μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C24H23CIF2N7O3 (M+H) ⁺ m/z = 530.2; found 530.2.

Example 61. 3-(6-Chloro-3-((l-(3-cyclopentyl-4,7-dimethyl-5-oxo-4,5-dihy dro-3H- pyrazolo[3,4-c]isoquinolin-9-yl)ethyl)amino)pyridin-2-yl)-l, 2,4-oxadiazol-5(4H)-one

The titled compound was prepared using similar procedures as described for Example 16 with 1 -cyclopentyl -IH-pyrazol -5 -amine replacing 1 -methyl -IH-pyrazol -5 -amine in Step 1 and 3-(3-amino-6-chloropyridin-2-yl)-l,2,4-oxadiazol-5(4H)-one replacing 2-aminobenzoic acid in Step 7. The resulting mixture was purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5 μm; mobile phase A: water (0. 1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C26H27CIN7O3 (M+H) ⁺ m/z = 520.2; found 520.2.

Example 62. 3-(6-Chloro-3-((l-(3-isopropyl-4,7-dimethyl-5-oxo-4,5-dihydr o-3H- pyrazolo[3,4-c]isoquinolin-9-yl)ethyl)amino)pyridin-2-yl)-l, 2,4-oxadiazol-5(4H)-one

The titled compound was prepared using similar procedures as described for Example 16 with 1 -isopropyl- lH-pyrazol-5 -amine replacing l-methyl-lH-pyrazol-5-amine in Step 1 and 3-(3-amino-6-chloropyridin-2-yl)-l,2,4-oxadiazol-5(4H)-one replacing 2-aminobenzoic acid in Step 7. The resulting mixture was purified by prep-HPLC (column: Sunfire prep Cl 8 column, 30* 150 mm, 5 μm; mobile phase A: water (0. 1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C24H25CIN7O3 (M+H) ⁺ m/z = 494.2; found 494.2.

Example 63. 3-(6-Chloro-3-((l-(7-chloro-3-ethyl-4-methyl-5-oxo-4,5-dihyd ro-3H- pyrazolo[3,4-c]isoquinolin-9-yl)ethyl)amino)pyridin-2-yl)-l, 2,4-oxadiazol-5(4H)-one

The titled compound was prepared using similar procedures as described for Example 16 with l-ethyl-lH-pyrazol-5-amine replacing l-methyl-lH-pyrazol-5-amine in Step 1, 3- bromo-5-chloro-2 -iodobenzoic acid replacing 3-bromo-2-iodo-5-methylbenzoic acid in Step 1, and 3-(3-amino-6-chloropyridin-2-yl)-l,2,4-oxadiazol-5(4H)-one replacing 2- aminobenzoic acid in Step 7. The resulting mixture was purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5 μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C22H20CI2N7O3 (M+H) ⁺ m/z = 500.1; found 500.1.

Example 64. 3-(6-Chloro-3-((l-(7-chloro-3-(cyclopropylmethyl)-4-methyl-5 -oxo-4,5- dihydro-3H-pyrazolo[3,4-c]isoquinolin-9-yl)ethyl)amino)pyrid in-2-yl)-l,2,4-oxadiazol- 5(4H)-one

The titled compound was prepared using similar procedures as described for Example 16 with 1 -(cyclopropylmethyl) -IH-pyrazol -5 -amine replacing l-methyl-lH-pyrazol-5-amine in Step 1, 3-bromo-5-chloro-2 -iodobenzoic acid replacing 3 -bromo-2-iodo-5 -methylbenzoic acid in Step 1, and 3-(3-amino-6-chloropyridin-2-yl)-l,2,4-oxadiazol-5(4H)-one replacing 2- aminobenzoic acid in Step 7. The resulting mixture was purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5 μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C24H22CI2N7O3 (M+H) ⁺ m/z = 526.1; found 526.1.

Example 65. 3-(6-Chloro-3-((l-(4,7-dimethyl-5-oxo-3-propyl-4,5-dihydro-3 H- pyrazolo[3,4-c]isoquinolin-9-yl)ethyl)amino)pyridin-2-yl)-l, 2,4-oxadiazol-5(4H)-one

The titled compound was prepared using similar procedures as described for Example 16 with 1 -propyl -IH-pyrazol -5 -amine replacing l-methyl-lH-pyrazol-5-amine in Step 1 and 3-(3-amino-6-chloropyridin-2-yl)-l,2,4-oxadiazol-5(4H)-one replacing 2-aminobenzoic acid in Step 7. The resulting mixture was purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5 μm; mobile phase A: water (0. 1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C24H25CIN7O3 (M+H) ⁺ m/z = 494.2; found 494.2.

Example 66. 3-(6-Chloro-3-((l-(4,7-dimethyl-5-oxo-3-(tetrahydrofuran-3-y l)-4,5- dihydro-3H-pyrazolo[3,4-c]isoquinolin-9-yl)ethyl)amino)pyrid in-2-yl)-l,2,4-oxadiazol- 5(4H)-one The titled compound was prepared using similar procedures as described for Example 16 with l-(tetrahydrofiiran-3-yl)-lH-pyrazol-5-amine replacing l-methyl-lH-pyrazol-5- amine in Step 1, and 3-(3-amino-6-chloropyridin-2-yl)-l,2,4-oxadiazol-5(4H)-one replacing 2 -aminobenzoic acid in Step 7. The resulting mixture was purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5 μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C25H25CIN7O4 (M+H) ⁺ m/z = 522.2; found 522.2.

Example 67. 3-(6-Chloro-3-((l-(3-(2-methoxyethyl)-4,7-dimethyl-5-oxo-4,5 -dihydro-3H- pyrazolo[3,4-c]isoquinolin-9-yl)ethyl)amino)pyridin-2-yl)-l, 2,4-oxadiazol-5(4H)-one

The titled compound was prepared using similar procedures as described for Example 16 with l-(2-methoxyethyl)-lH-pyrazol-5 -amine replacing 1 -methyl -IH-pyrazol -5 -amine in Step 1 and 3-(3-amino-6-chloropyridin-2-yl)-l,2,4-oxadiazol-5(4H)-one replacing 2- aminobenzoic acid in Step 7. The resulting mixture was purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5 μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C24H25CIN7O4 (M+H) ⁺ m/z = 510.2; found 510.2.

Example 68. 3-(6-Chloro-3-((l-(3-(cyclopropylmethyl)-4,7-dimethyl-5-oxo- 4,5-dihydro-

3H-pyrazolo[3,4-c]isoquinolin-9-yl)ethyl)amino)pyridin-2- yl)-l,2,4-oxadiazol-5(4H)-one

The titled compound was prepared using similar procedures as described for Example

16 with 1 -(cyclopropylmethyl) -IH-pyrazol -5 -amine replacing l-methyl-lH-pyrazol-5-amine in Step 1 and 3-(3-amino-6-chloropyridin-2-yl)-l,2,4-oxadiazol-5(4H)-one replacing 2- aminobenzoic acid in Step 7. The resulting mixture was purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5 μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C25H25CIN7O3 (M+H) ⁺ m/z = 506.2; found 506.2.

Example 69. 3-(6-Chloro-3-((l-(3-ethyl-4-methyl-5-oxo-7-(trifluoromethyl )-4,5-dihydro- 3H-pyrazolo[3,4-c]isoquinolin-9-yl)ethyl)amino)pyridin-2-yl) -l,2,4-oxadiazol-5(4H)-one

The titled compound was prepared using similar procedures as described for Example 16 with 3-bromo-2-iodo-5-(trifluoromethyl)benzoic acid replacing 3-bromo-2-iodo-5- methylbenzoic acid in Step 1, l-ethyl-lH-pyrazol-5-amine replacing l-methyl-lH-pyrazol-5- amine in Step 1, and 3-(3-amino-6-chloropyridin-2-yl)-l,2,4-oxadiazol-5(4H)-one replacing 2 -aminobenzoic acid in Step 7. The resulting mixture was purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5 μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C23H20CIF3N7O3 (M+H) ⁺ m/z = 534.1; found 534.1.

Example 70. 3-(6-Chloro-3-((l-(3,8-dimethyl-6-oxo-4,5-dihydro-3H,6H-2,2a ,5a- triazaaceanthrylen-10-yl)ethyl)amino)pyridin-2-yl)-l,2,4-oxa diazol-5(4H)-one

The titled compound was prepared using similar procedures as described for Example

36 with 7-methyl-4,5,6,7-tetrahydropyrazolo[l,5-a]pyrimidine replacing 4, 5,6,7- tetrahydropyrazolo[ 1,5 -a] pyrimidine in Step 2 and 3-(3-amino-6-chloropyridin-2-yl)-l,2,4- oxadiazol-5(4H)-one replacing 2-aminobenzoic acid in Step 7. The resulting mixture was purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5 μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C24H23CIN7O3 (M+H) ⁺ m/z = 492.2; found 492.2.

Example 71. 3-(6-Chloro-3-((l-(3-ethyl-4,7-dimethyl-5-oxo-4,5-dihydroimi dazo[l,5- a] quinazolin-9-yl)ethyl)amino)pyridin-2-yl)-l,2,4-oxadiazol-5( 4H)-one

Step 1. 9-Acetyl-3-iodo-4, 7-dimethylimidazo[l,5-a]quinazolin-5(4H)-one tert-Butyl 9-acetyl-4,7-dimethyl-5-oxo-4,5-dihydroimidazo[l,5-a]quinazo line-3- carboxylate (Example 4, Step7) (3.0 g, 8.4 mmol) was dissolved in TFA (20 mb). The mixture was stirred for 3 h at rt. After completion, TFA was removed under vacuum. 200 mb of ice water and 50 mb of EtOAc were added to the reaction residue while stirring. After stirring for 20 min, the resulting solid was collected to afford a light brown solid, which was washed with water (2X). The solid was dried under vacuum to provide a beige solid. The solid product was dissolved in DMF (20 mb). To this mixture was added NaHCO, (742 mg, 8.8 mmol) at 0 °C, after stirring for 5 min, NIS (600 mg, 2.6 mmol) was added in one portion. The resulting mixture was warmed to rt and stirred for 2h under vacuum. After completion, the reaction was quenched with sat. Na2S2C>3 and diluted with water. The resulting solid was collected to afford a light brown solid, which was washed with water (2X). The solid was dried under vacuum to provide a beige solid. ECMS calculated for C14H13IN3O2 (M+H) ⁺ m/z = 382.0; found 382.0.

Step 2: 9-Acetyl-4, 7-dimethyl-3-vinylimidazo[l,5-a]quinazolin-5(4H)-one

The mixture of 9-acetyl-3-iodo-4,7-dimethylimidazo[l,5-a]quinazolin-5(4H)-o ne (2.0 g, 5.2 mmol), PdC12(PPh3)2 (360 mg, 0.5 mmol), and tributyl(vinyl)stannane (1.9 g, 6.0 mmol) in dioxane (20 mL) was heated at 100 °C for 2 h under nitrogen atmosphere. Upon cooling to room temperature, the mixture was diluted with water and extracted with EtOAc. The combined organics were washed with sat. NaCl, dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with DCM and ethyl acetate to provide the desired product as a brown solid (1.3 g, 90%). LCMS calculated for C16H16N3O2 (M+H) ⁺ m/z = 282.1; found 282.2.

Step 3. 9-Acetyl-3-ethyl-4, 7-dimethylimidazo[l,5-a]quinazolin-5(4H)-one

In a 250 mL flask, 9-acetyl-4,7-dimethyl-3-vinylimidazo[l,5-a]quinazolin-5(4H)- one (1.3 g, 4.6 mmol) and Pd/C (10 wt% on active carbon, 200 mg) was dissolved in MeOH (150 mL). The flask was then charged with latm H2 and stirred for 18 h. The reaction mixture was then filtrate through a short pad of celite and solvent was removed under vacuum to afford the crude product which was used in next step without further purification.

Step 4. 3-Ethyl-9-(l-hydroxyethyl)-4, 7-dimethylimidazo [1 ,5-a] quinazolin-5(4H)-one

9-Acetyl-3-ethyl-4,7-dimethylimidazo[l,5-a]quinazolin-5(4 H)-one (1.0 g, 3.5 mmol) was dissolved in MeOH (100 mL) and DCM (100 mL) then NaBHt (182 mg, 4.8 mmol) was added at 0 °C in three portions under nitrogen. The resulting mixture was stirred at same temperature for 10 min before quenched with sat. NH4CI. The mixture was diluted with water and extracted with DCM. The combined organics were washed with sat. NaCl, and dried with anhydrous sodium sulfate, fdtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with DCM and MeOH (0 to 4%) to provide the desired product as a light yellow solid (798 mg, 80%). LCMS calculated for C16H20N3O2 (M+H) ⁺ m/z = 286.2; found 286.2.

Step 5. 9-(l-Bromoethyl)-3-ethyl-4, 7-dimethylimidazo[l,5-a]quinazolin-5(4H)-one

To a mixture of 3-ethyl-9-(l-hydroxyethyl)-4,7-dimethylimidazo[l,5-a]quinazo lin- 5(4H)-one (100 mg, 0.35 mmol) in DCM (200 mL) was added PBrs (57 uL, 0.6 mmol) at 0 °C. The resulting mixture was warmed to rt and stirred for 2 h. The mixture was then quenched with sat. NaHCCh and extracted with DCM (2x). The combined organics were washed with sat. NaCl, and dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was used directly in next step without further purification.

Step 6. 3-(6-Chloro-3-((l-(3-ethyl-4, 7-dimethyl-5-oxo-4,5-dihydroimidazo[l,5-a]quinazolin- 9-yl)ethyl)amino)pyridin-2-yl)-l,2,4-oxadiazol-5(4H)-one

To the solution of 9-(l-bromoethyl)-3-ethyl-4,7-dimethylimidazo[l,5-a]quinazoli n- 5(4H)-one (10 mg, 0.03 mmol) in DMF (0.3 mL) was added 3-(3-amino-6-chloropyridin-2- yl)-l,2,4-oxadiazol-5(4H)-one (20 mg, 0.09 mmol). The mixture was heated to 80 °C for 2 h. Upon cooling to room temperature, the mixture was diluted with MeOH and purified by prep- HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C23H23CIN7O3 (M+H) ⁺ m/z = 480.2; found 480.2.

Example 72. 9-(l-((2-(lH-Tetrazol-5-yl)pyridin-3-yl)amino)ethyl)-3-ethyl -4,7-dimethyl-

3,4-dihydro-5H-pyrazolo [3,4-c] isoquinolin-5-one

Step 1. 2-(lH-Tetrazol-5-yl)pyridin-3-amine

To a solution of 3-aminopicolinonitrile (1.18g, 10 mmol) in DMAc (20 mL) was added sodium azide (1.30 g, 20 mmol) and ammonium chloride (1.07g, 20 mmol) in one portion. The suspension was heated to 120 °C, stirred overnight and cooled to rt. The solvent was removed under reduced pressure. The residue was dissolved in 10 mL water, neutralized with 10% HC1 and filtered off. The precipitate was washed with water and dried to afford the desired product as a yellow solid (1.0 g, 64% yield). LCMS calculated for CeFfiNe (M+H) ⁺ m/z = 163.1; found 163.1.

Step 2. 9-(l-((2-(lH-Tetrazol-5-yl)pyridin-3-yl)amino)ethyl)-3-ethyl -4, 7 -dimethyl- 3,4- dihydro-5H-pyrazolo[3, 4-c]isoquinolin-5-one

The titled compound was prepared using similar procedures as described for Example 16 with l-ethyl-lH-pyrazol-5-amine replacing l-methyl-lH-pyrazol-5-amine in Step 1 and 2- (lH-tetrazol-5-yl)pyridin-3-amine replacing 2-aminobenzoic acid in Step 7. The resulting mixture was purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5 μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C22H24N9O (M+H) ⁺ m/z = 430.2; found 430.2.

Example 73. 6-Chloro-3-((l-(3-ethyl-4,7-dimethyl-5-oxo-4,5-dihydro-3H-py razolo [3,4- c]isoquinolin-9-yl)ethyl)amino)-N-(l-methylpiperidin-4-yl)pi colinamide

To a solution of 6-chloro-3-((l-(3-ethyl-4,7-dimethyl-5-oxo-4,5-dihydro-3H- pyrazolo[3,4-c]isoquinolin-9-yl)ethyl)amino)picolinic acid (Example 19) (7 mg, 0.016 mmol) in DMF (1 ml) was added HATU (9 mg, 0.023 mmol) at room temperature. The resulting mixture was stirred for 15 minutes then l-methylpiperidin-4-amine hydrochloride (3.5 mg, 0.023 mmol) and triethylamine (11.1 uL, 0.08 mmol) were added sequentially. The mixture was stirred at room temperature for 2 hours. The mixture was diluted MeOH and purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a yellow solid. LCMS calculated for C28H35CIN7O2 (M+H) ⁺ m/z = 536.2; found 536.2.

The following compounds in Table 6 were prepared similarly as described for Example 73.

Table 6. Example 76. 9-(l-((6-Chloro-3'-fluoro-[2,4'-bipyridin]-3-yl)amino)-2-hyd roxyethyl)-3- ethyl-4,7-dimethyl-3,4-dihydro-5H-pyrazolo[3,4-c]isoquinolin -5-one

Step 1. 9-Bromo-3-ethyl-4, 7 -dimethyl- 3, 4-dihydro-5H-pyrazolo [3,4-c]isoquinolin-5-one

The titled compound was prepared using similar procedures as described for Example 16 with l-ethyl-lH-pyrazol-5-amine replacing l-methyl-lH-pyrazol-5-amine in Step 1. The resulting mixture was purified with silica gel chromatography, eluting with 0% to 100% EtOAc in DCM to give the desired product as a beige solid. LCMS calculated for Ci ₄ Hi ₅ BrN ₃ O (M+H) ⁺ m/z = 320.0; found 320.0.

Step 2. 3-Ethyl-4, 7-dimethyl-9-vinyl-3,4-dihydro-5H-pyrazolo[3,4-c]isoquinolin -5-one

The mixture of 9-bromo-3-ethyl-4,7-dimethyl-3,4-dihydro-5H-pyrazolo[3,4- c]isoquinolin-5-one (500 mg, 1.6 mmol), PdC12(PPh3)2 (110 mg, 0.16 mmol), and tributyl(vinyl)stannane (500 mg, 1.6 mmol) in dioxane (5 mL) was heated at 100 °C for 2 h under nitrogen atmosphere. Upon cooling to room temperature, the mixture was diluted with water and extracted with EtOAc. The combined organics were washed with sat. NaCl, dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with DCM and ethyl acetate to provide the desired product as a brown solid. LCMS calculated for C’ I ₍ ,H IXN;O (M+H) ⁺ m/z = 268.0; found 268.1.

Step 3. 3-Ethyl-4, 7-dimethyl-9-(oxiran-2-yl)-3, 4-dihydro-5H-pyrazolo [3, 4-c]isoquinolin-5- one

To a mixture of 3-ethyl-4,7-dimethyl-9-vinyl-3,4-dihydro-5H-pyrazolo[3,4- c]isoquinolin-5-one (400 mg, 1.50 mmol) in DCM (50 mL) was added mCPBA (489 mg, 2.0 mmol) at 0 °C in three portions over Ih. The reaction mixture was then warmed to rt and stirred for 2 h. After completion, the reaction was diluted with ice water and washed with sat NaHCCL and sat. NaCl, dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure to give the title compound as a light-yellow oil. The crude product was used in the next step without further purification.

Step 4. 9-(l-((2-Bromo-6-chloropyridin-3-yl)amino)-2-hydroxyethyl)-3 -ethyl-4, 7-dimethyl-

3.4-dihydro-5H-pyrazolo[3, 4-c]isoquinolin-5-one

To a mixture of 3-ethyl-4,7-dimethyl-9-(oxiran-2-yl)-3,4-dihydro-5H-pyrazolo [3,4- c]isoquinolin-5-one (100 mg, 0.35 mmol) in acetonitrile (10 mL) was added 2-bromo-6- chloropyridin-3 -amine (100 mg, 0.48 mmol) and Sc(0Tf)3 (20 mg, 0.04 mmol) at rt. The reaction mixture was then warmed to rt and stirred for 2 h. After completion, the reaction was diluted with ice water and washed with sat NaHCCh and sat. NaCl, dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure to give the crude product. The residue was purified by silica gel column chromatography, eluted with DCM and MeOH to provide the desired product as a brown solid. LCMS calculated for C2iH22BrClN5C>2 (M+H) ⁺ m/z = 490.0; found 490. 1.

Step 5. 9-(l-((6-Chloro-3'-fluoro-[2,4'-bipyridin]-3-yl)amino)-2-hyd roxyethyl)-3-ethyl-4, 7- dimethyl-3, 4-dihydro-5H-pyrazolo[3, 4-c]isoquinolin-5-one

The mixture of 9-(l-((2-bromo-6-chloropyridin-3-yl)amino)-2-hydroxyethyl)-3 -ethyl- 4,7-dimethyl-3,4-dihydro-5H-pyrazolo[3,4-c]isoquinolin-5-one (10 mg, 0.02 mmol), Pd(PPh3)4 (3 mg, 0.003 mmol), and (3-fluoropyridin-4-yl)boronic acid (4 mg, 0.03 mmol) in dioxane (0.5 mL) and water (0. 1 mL) was heated at 80°C for 10 min under nitrogen atmosphere. Upon cooling to room temperature, the mixture was diluted with MeOH and purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C ₂ 6H25C1FN ₆ O ₂ (M+H) ⁺ m/z = 507.2; found 507.2.

The following compounds in Table 7 were prepared similarly as described for Example 76.

Table 7.

Example 79. 9-(l-((6-Chloro-2-(l-(l-methylpiperidin-4-yl)-lH-pyrazol-4-y l)pyridin-3- yl)amino)ethyl)-3-ethyl-4,7-dimethyl-3,4-dihydro-5H-pyrazolo [3,4-c]isoquinolin-5-one

The title compound was prepared using similar procedures as described for Example 41 with l-methyl-4-(4-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl)- lH-pyrazol-l- yl)piperidine replacing l-methyl-5-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl)pyri din-2- one in Step 2. The resulting mixture was purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5 μm; mobile phase A: water (0. 1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for CsoFfieClNgO (M+H) ⁺ m/z = 559.2; found 559.2.

Example 80. 9-(l-((2-(4-Acetylpiperazin-l-yl)-6-chloropyridin-3-yl)amino )ethyl)-3-ethyl- 4,7-dimethyl-3,4-dihydro-5H-pyrazolo[3,4-c]isoquinolin-5-one

Step 1: 9-(l-((6-chloro-2-fluoropyridin-3-yl)amino)ethyl)-3-ethyl-4, 7 -dimethyl- 3, 4-dihydro- 5H-pyrazolo[3, 4-c]isoquinolin-5-one The title compound was prepared using similar procedures as described for Example 16 with 6-chloro-2-fluoropyridin-3-amine replacing 2-aminobenzoic acid in Step 7 and 1- ethyl-lH-pyrazol-5-amine replacing 1 -methyl -IH-pyrazol -5 -amine in Step 1. The crude product was purified with silica gel chromatography, eluting with 0% to 100% EtOAc in DCM to give the desired product as a beige solid. LCMS calculated for C21H22CIFN5O (M+H) ⁺ m/z = 414.1; found 414.

Step 2: 9-(l-((2-(4-acetylpiperazin-l-yl)-6-chloropyridin-3-yl)amino )ethyl)-3-ethyl-4, 7- dimethyl-3, 4-dihydro-5H-pyrazolo[3, 4-c]isoquinolin-5-one

To a solution of 9-(l-((6-chloro-2-fhioropyridin-3-yl)amino)ethyl)-3-ethyl-4, 7- dimethyl-3,4-dihydro-5H-pyrazolo[3,4-c]isoquinolin-5-one (10.0 mg , 0.02 mmol) in anhydrous DMF (0.3 mb) was added A-cthyl-A-isopropyl-propan-2 -amine (37.0 uh, 0.22 mmol) and 1 -(piperazin- l-yl)ethan-l -one (11.2 uh, 0.09 mmol). The resulting mixture was heated at 100 °C for 3 hours. Upon completion of reaction, the residue was diluted with methanol and purified by prep-HPEC (column: Sunfire prep C18 column, 30* 150 mm, 5 μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C27H33CIN7O2 (M+H) ⁺ m/z = 522.2; found 522.2.

Example 81 : 9-(l-((6-Chloro-2-(l-methyllH-pyrazol-4-yl)pyridin-3-yl)amin o)ethyl)-4,7- dimethyl-3-(l-methylazetidin-3-yl)-3,4-dihydro-5H-pyrazolo[3 ,4-c]isoquinolin-5-one

Step 1: tert-butyl 3-hydrazineylazetidine-l -carboxylate

Boc

To a 500 mb seal flask was added tert-butyl 3-((methylsulfonyl)oxy)azetidine-l- carboxylate (20 g, 79.7 mmol) and hydrazine monohydrate (50 mb). The mixture was then heated at 100 °C for 20 h. After cooled to rt, the reaction mixture was diluted with 50 mb of water and extracted with EtOAc (4x). The combined organics were dried with anhydrous sodium sulfate, fdtered, and concentrated under reduced pressure. The residue was used directly in the next step without further purification.

Step 2: ethyl 5-amino-l-(l-(tert-butoxycarbonyl)azetidin-3-yl)-lH-pyrazole -4-carboxylate

To a 500 mL flask was added tert-butyl 3 -hydrazineylazetidine-1 -carboxylate (15 g, 80 mmol) and ethyl (E)-2-cyano-3 -ethoxyacrylate (16.26 g, 96 mmol) and EtOH (500 mL). The mixture was then heated at 85 °C for 15 h. After cooled to rt, the reaction mixture was concentrated under reduced pressure. The resulting crude product was stirred in 200 mL of DCM. The resulting solid was collected after filtration and used directly in the next step without further purification.

Step 3: tert-butyl 3-(5-amino-lH-pyrazol-l-yl)azetidine-l-carboxylate Boc

To a 500 mL flask was added ethyl 5-amino-l-(l-(tert-butoxycarbonyl)azetidin-3-yl)- IH-pyrazole -4 -carboxylate (20 g, 64 mmol), EtOH (500 mL) and 5N NaOH solution (100 mL). The mixture was then heated at 85 °C for 2 h. After cooled to rt, the reaction mixture was concentrated under reduced pressure. The resulting residue was acidified by adding 6N HC1 slowly at 0 °C. The resulting solid was collected after filtration and dried under vacuum to afford a white solid. To the solid was added chlorobenzene (400 mL), and the resulting mixture was heated at 140 °C for 12h. After completion, the solvent was removed under vacuum and the residue was used directly in next step as crude product.

Step 4: tert-butyl 3-(5-(3-bromo-2-iodo-5-methylbenzamido)-lH-pyrazol-l-yl)azet idine-l- carboxylate To a 500 mL flask was added tert-butyl 3-(5-amino-lH-pyrazol-l-yl)azetidine-l- carboxylate (12 g, 50 mmol), 3 -bromo-2-iodo-5 -methylbenzoic acid (19.4 g, 57 mmoL) and THF (500 mL). To a mixture was then added propanephosphonic acid anhydride (50% THF solution, 50 mL) and DIEA (13 mL, 71 mmol). The reaction mixture was then heated at 70 °C overnight. After completion, the solvent was removed under vacuum. The residue was diluted with EtOAc, washed with sat. NaHCCE and brine. The combined organic layer was dried over Na2SC>4 and concentrated under vacuum. The residue was purified by silica gel column chromatography, eluted with DCM and MeOH (0 to 4%) to provide the desired product as a brown solid (14 g, 50%). LCMS calculated for CigffaBrlbLOs (M+H) ⁺ m/z = 561.0; found 561.0.

Step 5: tert-butyl 3-(5-(3-bromo-2-iodo-N,5-dimethylbenzamido)-lH-pyrazol-l-yl) azetidine- 1 -carboxylate Boc

To a mixture of tert-butyl 3-(5-(3-bromo-2-iodo-5-methylbenzamido)-lH-pyrazol-l- yl)azetidine-l -carboxylate (14 g, 25 mmol) in DMF (300 mL) was added NaH (1.2 g, 30 mmol) at 0 °C under nitrogen atmosphere. The resulting mixture was stirred at same temperature for 30 min and then Mel (4.3 g, 30 mmol) was added. The reaction mixture was then slowly warmed to rt and stirred for 1 h. After completion, the reaction mixture was carefully quenched with water at 0 °C while vigorously stirred. The mixture was then extracted with ethyl acetate. The combined organics were dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with hexanes and ethyl acetate to provide the desired product, which was further purified by silica gel column chromatography, eluted with DCM and EtOAc to provide the desired product as a white solid (11.4 g, 80%). LCMS calculated for C ₂ oH25BrIN ₄ 03 (M+H) ⁺ m/z = 575.1; found 575.0.

Step 6: tert-butyl 3-(9-bromo-4, 7-dimethyl-5-oxo-4,5-dihydro-3H-pyrazolo[3,4-c]isoquinolin- 3-yl)azetidine-l -carboxylate

A mixture of tert-butyl 3-(5-(3-bromo-2-iodo-5-methylbenzamido)-lH-pyrazol-l- yl)azetidine-l -carboxylate (11.4 g, 20 mmol), Pd(PPhs)4 (6.9 g, 6 mmol), KO Ac (11.7 g, 120 mmol) in DMAc (200 mL) was heated at 105 °C for 24 h under nitrogen atmosphere. Upon cooling to room temperature, the mixture was diluted with water and extracted with EtOAc. The combined organics were washed with sat. NaCl, dried with anhydrous sodium sulfate and filtered. The filtrate was concentrated and used directly in the next step without future purification. LCMS calculated for C2oH24BrN403 (M+H) ⁺ m/z = 447.1; found 447.1.

Step 7: tert-butyl 3-(9-acetyl-4, 7-dimethyl-5-oxo-4,5-dihydro-3H-pyrazolo[3,4-c]isoquinolin- 3-yl)azetidine-l -carboxylate

A mixture of tert-butyl 3-(9-bromo-4,7-dimethyl-5-oxo-4,5-dihydro-3H- pyrazolo[3,4-c]isoquinolin-3-yl)azetidine-l -carboxylate (5.3 g, 12 mmol), PdC12(PPh3)2 (842 mg, 1.2 mmol), and tributyl(l-ethoxyvinyl)stannane (4.4 g, 14 mmol) in dioxane (200 mL) was heated at 100 °C for 8 h under nitrogen atmosphere. Upon cooling to room temperature, to the mixture was added 2 N HC1 and stirred for 30 min before the mixture was diluted with water and extracted with EtOAc. The combined organics were washed with sat. NaCl and dried with anhydrous sodium sulfate, fdtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with DCM and ethyl acetate to provide the desired product as a white solid (3.4 g, 70%). LCMS calculated for C22H27N4O4 (M+H) ⁺ m/z = 411.2; found 411.2.

Step 8: tert-butyl 3-(9-(l-hydroxyethyl)-4, 7-dimethyl-5-oxo-4,5-dihydro-3H-pyrazolo[3,4- c]isoquinolin-3-yl)azetidine-l-carboxylate To a mixture of tert-butyl 3-(9-acetyl-4,7-dimethyl-5-oxo-4,5-dihydro-3H- pyrazolo[3,4-c]isoquinolin-3-yl)azetidine-l-carboxylate (3.4 g, 8.3 mmol) in MeOH (100 mL) and DCM (100 mL) was added NaBfL (342 mg, 9 mmol) at 0 °C. The resulting mixture was stirred at same temperature for 10 min before quenched with sat. NH4CI. The mixture was diluted with water and extracted with DCM/IPA 3: 1. The combined organics were washed with sat. NaCl and dried with anhydrous sodium sulfate, fdtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with DCM and MeOH to provide the desired product as a white solid (2.8 g, 82%). LCMS calculated for C22H29N4O4 (M+H) ⁺ m/z = 413.2; found 413.2.

Step 9: tert-butyl 3-(9-(l-bromoethyl)-4, 7-dtmethyl-5-oxo-4,5-dthydro-3H-pyrazolo[3,4- c]isoquinolin-3-yl)azetidine-l-carboxylate

To a mixture of tert-butyl 3-(9-(l-hydroxyethyl)-4,7-dimethyl-5-oxo-4,5-dihydro-3H- pyrazolo[3,4-c]isoquinolin-3-yl)azetidine-l -carboxylate (500 mg, 1.2 mmol) in THF (50 mL) was added pyridine (0.29 mL, 3.6 mmol) and PBr, (648 mg, 2.4 mmol). The resulting mixture was stirred at rt for 2 h. The mixture was then quenched with sat. NaHCO; and extracted with EtOAc. The combined organics were washed with sat. NaCl, dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was used directly in the next step without further purification.

Step 10: 3-(azetidin-3-yl)-9-(l-((6-chloro-2-(l-methyl-lH-pyrazol-4-y l)pyridin-3- yl)amino)ethyl)-4, 7-dimethyl-3, 4-dihydro-5H-pyrazolo[3, 4-c]isoquinolin-5-one

To tert-butyl 3-(9-(l-bromoethyl)-4,7-dimethyl-5-oxo-4,5-dihydro-3H-pyrazo lo[3,4- c]isoquinolin-3-yl)azetidine-l-carboxylate (20 mg, 0.042 mmol) in 2 dram vial was added DMF (0.2 mL), and 6-chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3-amine (20 mg, 0.096 mmol). The mixture was heated to 80 °C for 1 h. Upon cooling to room temperature, TFA (0.5 mL) was added to the mixture. The mixture was stirred for 30 min before diluted with MeOH and purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C26H28CIN8O (M+H) ⁺ m/z = 503.2; found 503.2.

Step 11: 9-(l-((6-chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3-yl)ami no)ethyl)-4, 7-dimethyl- 3-(l-methylazetidin-3-yl)-3, 4-dihydro-5H-pyrazolo[3, 4-c]isoquinolin-5-one

To a mixture of 3-(azetidin-3-yl)-9-(l-((6-chloro-2-(l-methyl-lH-pyrazol-4- yl)pyridin-3 -yl)amino)ethyl)-4,7-dimethyl-3 ,4-dihydro-5H-pyrazolo [3 ,4-c] isoquinolin-5 -one (5 mg, 0.01 mmol) was added DCM (0.5 mL), formaldehyde (water solution, 10 uL) and sodium triacetoxyborohydride (5 mg, 0.023 mmol). The mixture was stirred for 30 min, then diluted with MeOH and purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C27H30CIN8O (M+H) ⁺ m/z = 517.2; found 517.2.

Examples 82-88.

Examples 82-88 in Table 8 were prepared similarly as described for Example 81.

Table 8.

Example 89: 9-(l-((6-chloro-2-(l-methyl-lH-l,2,4-triazol-3-yl)pyridin-3- yl)amino)ethyl)-

3-(l-(2-hydroxyethyl)azetidin-3-yl)-4,7-dimethyl-3,4-dihy dro-5H-pyrazolo[3,4- c]isoquinolin-5-one

Step 1 : 3-(azetidin-3-yl)-9-(l -(( 6-chloro-2-(l -methyl- 1H- 1,2, 4-triazol-3-yl)pyridin-3- yl)amino)ethyl)-4, 7-dimethyl-3, 4-dihydro-5H-pyrazolo [3, 4-c]isoquinolin-5-one

The title compound was prepared using similar procedures as described for Example 81 with 6-chloro-2-( 1 -methyl- 1H- 1 ,2,4-triazol-3 -yl)pyridin-3 -amine replacing 6-chloro-2-( 1 - methyl-lH-pyrazol-4-yl)pyridin-3-amine in Step 10. The resulting mixture was purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5 μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C25H27CIN9O (M+H) ⁺ m/z = 504.2; found 504.2. Step 2: 9-(l-((6-chloro-2-(l-methyl-lH-l,2, 4-triazol-3-yl)pyridin-3-yl)amino)ethyl)-3-(l-(2- hydroxyethyl)azetidin-3-yl)-4, 7-dimethyl-3, 4-dihydro-5H-pyrazolo[3, 4-c]isoquinolin-5-one

To a mixture of 3-(azetidin-3-yl)-9-(l-((6-chloro-2-(l-methyl-lH-l,2,4-triaz ol-3- yl)pyridin-3 -yl)amino)ethyl)-4,7-dimethyl-3 ,4-dihydro-5H-pyrazolo [3 ,4-c] isoquinolin-5 -one (10 mg, 0.02 mmol) was added DCM (0.5 mL), 2-((tert-butyldimethylsilyl)oxy)acetaldehyde (10 mg, 0.05 mmol) and sodium triacetoxyborohydride (5 mg, 0.023 mmol). The mixture was stirred for 30 min, solvent was removed under vacuum. 4 M HC1 in dioxane (0.3 mL) and MeOH (0.2 mL) was added and the mixture was stirred for 10 min before diluted with MeOH and purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C27H31CIN9O2 (M+H) ⁺ m/z = 548.2; found 548.2.

Example 90: 9-(l-((6-chloro-2-(l-methyl-lH-l,2,4-triazol-3-yl)pyridin-3- yl)amino)ethyl)- 3-(l-cyclopropylazetidin-3-yl)-4,7-dimethyl-3,4-dihydro-5H-p yrazolo[3,4-c]isoquinolin- 5-one

To a mixture of 3-(azetidin-3-yl)-9-(l-((6-chloro-2-(l-methyl-lH-l,2,4-triaz ol-3- yl)pyridin-3 -yl)amino)ethyl)-4,7-dimethyl-3 ,4-dihydro-5H-pyrazolo [3 ,4-c] isoquinolin-5 -one (10 mg, 0.02 mmol) was added MeOH (0.5 mL), (1 -ethoxy cyclopropoxy)trimethylsilane (30 mg, 0.17 mmol), sodium cyanoborohydride (10 mg, 0.16 mmol) and acetic acid (10 uL). The mixture was stirred for 12 h before diluted with MeOH and purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5μm; mobile phase A: water (0. 1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C28H31CIN9O (M+H) ⁺ m/z = 544.2; found 544.2. Example 91: 9-(l-((6-chloro-2-(l-methyl-lH-l,2,4-triazol-3-yl)pyridin-3- yl)amino)ethyl)-

3-(l-((S)-2-hydroxypropyl)azetidin-3-yl)-4,7-dimethyl-3,4 -dihydro-5H-pyrazolo[3,4- c]isoquinolin-5-one To a mixture of 3-(azetidin-3-yl)-9-(l-((6-chloro-2-(l-methyl-lH-l,2,4-triaz ol-3- yl)pyridin-3 -yl)amino)ethyl)-4,7-dimethyl-3 ,4-dihydro-5H-pyrazolo [3 ,4-c] isoquinolin-5 -one (10 mg, 0.02 mmol) was added acetonitrile (0.5 mL), (S)-2 -methyloxirane (10 mg, 0.17 mmol) and DIEA(10 uL). The mixture was stirred at 70 °C for 6 h before diluted with MeOH and purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C28H33CIN9O2 (M+H) ⁺ m/z = 562.2; found 562.2.

Examples 92-96.

Examples 92-96 in Table 9 were prepared similarly as described for Example 91.

Table 9.

Example 97: 3-(l-Acetylazetidin-3-yl)-9-(l-((6-chloro-2-(l-methyl-lH-l,2 ,4-triazol-3- yl)pyridin-3-yl)amino)ethyl)-4,7-dimethyl-3,4-dihydro-5H-pyr azolo[3,4-c]isoquinolin-5- one

To a mixture of 3-(azetidin-3-yl)-9-(l-((6-chloro-2-(l-methyl-lH-l,2,4-triaz ol-3- yl)pyridin-3 -yl)amino)ethyl)-4,7-dimethyl-3 ,4-dihydro-5H-pyrazolo [3 ,4-c] isoquinolin-5 -one (Example 89, step 1) (10 mg, 0.02 mmol) in DCM (0.5 mL) was added acetyl chloride (3.2 mg, 0.04 mmol) DCM (0.2 mL) solution and A.A-diisopropylcthylaminc (5.0 uL) at 0 °C. The reaction mixture was stirred at same temperature for 30 minutes before the solvent was removed under vacuum. The resulting mixture was diluted with methanol and purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5 μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C27H29CIN9O2 (M+H) ⁺ m/z = 546.2; found 546.2.

Examples 98-99.

Examples 98-99 in Table 10 were prepared similarly as described for Example 97

Table 10.

Example 100: 3-(l-Acetylazetidin-3-yl)-9-(l-((6-chloro-l'-methyl-6'-oxo-l ',6'-dihydro- [2,3'-bipyridin]-3-yl)amino)ethyl)-4,7-dimethyl-3,4-dihydro- 5H-pyrazolo[3,4- c]isoquinolin-5-one

Step 1 : 9-acetyl-3-(azetidin-3-yl)-4, 7-dimethyl-3, 4-dihydro-5H-pyrazolo [3, 4-c]isoquinolin-5- one

To tert-butyl 3-(9-acetyl-4,7-dimethyl-5-oxo-4,5-dihydro-3H-pyrazolo[3,4- c]isoquinolin-3-yl)azetidine-l-carboxylate (Example 81, step 7) (200 mg, 0.57 mmol) in 2 dram vial was added TFA (0.5 mL). The mixture was stirred at rt for 1 h. The solvent was removed under vacuum. The residue was washed with sat. NaHCCE. then extracted with DCM/IPA 3: 1. The combined organic layer was dried over Na2SC>4 and concentrated under vacuum. The residue was used directly in the next step without further purification. LCMS calculated for C17H19N4O2 (M+H) ⁺ m/z = 311.1; found 311.2. Step 2: 9-acetyl-3-(l-acetylazetidin-3-yl)-4, 7-dimethyl-3,4-dihydro-5H-pyrazolo[3,4- c ] isoquinolin-5-one

To a mixture of 9-acetyl-3-(azetidin-3-yl)-4,7-dimethyl-3,4-dihydro-5H-pyraz olo[3,4- c]isoquinolin-5-one (110 mg, 0.37 mmol) in DCM (3 mL) was added acetyl chloride (32 mg, 0.4 mmol) DCM (2 mL) solution and A.A-diisopropylcthylaminc (110 uL 0.6 mmol) at 0 °C. The reaction mixture was stirred at same temperature for 30 minutes before the solvent was removed under vacuum. The resulting mixture was as washed with sat. NaHCO,. then extracted with EtOAc. The combined organic layer was dried over Na2SC>4 and concentrated under vacuum. The residue was used directly in the next step without further purification. LCMS calculated for C19H21N4O3 (M+H) ⁺ m/z = 353.2; found 353.2.

Step 3: 3-(l-acetylazetidin-3-yl)-9-(l-hydroxyethyl)-4, 7 -dimethyl- 3, 4-dihydro-5H- pyrazolo[3, 4-c]isoquinolin-5-one

To a mixture of 9-acetyl-3-(l-acetylazetidin-3-yl)-4,7-dimethyl-3,4-dihydro- 5H- pyrazolo[3,4-c]isoquinolin-5-one (80 mg, 0.23 mmol) in MeOH (1 mL) and DCM (1 mL) was added NaBfL (12 mg, 0.3 mmol) at 0 °C. The resulting mixture was stirred at same temperature for 10 min before quenched with sat. NH4CI. The mixture was diluted with water and extracted with DCM/IPA 3: 1. The combined organics were washed with sat. NaCl and dried with anhydrous sodium sulfate, fdtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with DCM and MeOH to provide the desired product as a white solid (73 mg, 90%). LCMS calculated for C19H23N4O3 (M+H) ⁺ m/z = 355.2; found 355.2.

Step 4: 3-(l-acetylazetidin-3-yl)-9-(l-bromoethyl)-4, 7-dimethyl-3,4-dihydro-5H- pyrazolo[3, 4-c]isoquinolin-5-one

To a mixture of 3-(l-acetylazetidin-3-yl)-9-(l-hydroxyethyl)-4,7-dimethyl-3, 4- dihydro-5H-pyrazolo[3,4-c]isoquinolin-5-one (73 mg, 0.21 mmol) in THF (1 mL) was added pyridine (29 uL, 0.36 mmol) PBr, (65 mg, 0.24 mmol). The resulting mixture was stirred at rt for 2 h. The mixture was then quenched with sat. NaHCCf and extracted with EtOAc. The combined organics were washed with sat. NaCl, dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was used directly in the next step without further purification.

Step 5: 3-(l-acetylazetidin-3-yl)-9-(l-((6-chloro-l '-methyl-6'-oxo-l ',6'-dihydro-[2,3'- bipyridin] -3-yl)amino)ethyl)-4, 7-dimethyl-3,4-dihydro-5H-pyrazolo[3,4-c]isoquinolin-5-one

3-( 1 -Acetylazetidin-3 -yl)-9-( 1 -bromoethyl)-4,7 -dimethyl-3,4-dihydro-5H- pyrazolo[3,4-c]isoquinolin-5-one (10 mg, 0.024 mmol) in 2 dram vial was added DMF (0.2 mL), and 3-amino-3-amino-6-chloro-r-methyl-[2,3'-bipyridin]-6'(rH)-on e (intermediate 24) (10 mg, 0.04 mmol). The mixture was heated to 80 °C for 1 h, then diluted with MeOH and purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C30H31CIN7O3 (M+H) ⁺ m/z = 572.2; found 572.2.

Examples 101-102.

Examples 101-102 in Table 11 were prepared similarly as described for Example 100.

Table 11.

Example 103: 9-(l-((6-Chloro-2-(l-methyl-lH-l,2,4-triazol-3-yl)pyridin-3- yl)amino)ethyl)-3-(l-(2-hydroxyacetyl)azetidin-3-yl)-4,7-dim ethyl-3,4-dihydro-5H- pyrazolo [3,4-c] isoquinolin-5-one

To a mixture of 3-(azetidin-3-yl)-9-(l-((6-chloro-2-(l-methyl-lH-l,2,4-triaz ol-3- yl)pyridin-3 -yl)amino)ethyl)-4,7-dimethyl-3 ,4-dihydro-5H-pyrazolo [3 ,4-c] isoquinolin-5 -one (Example 89, step 1) (10 mg, 0.02 mmol) was added DMF (0.5 mL), 2-hydroxyacetic acid (10 mg, 0.13 mmol), HATU (10 mg, 0.026 mmol) and DIEA(10 uL) at 0 °C. The mixture was warmed to rt and stirred for 2 h before diluted with MeOH and purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C27H29CIN9O3 (M+H) ⁺ m/z = 562.2; found 562.2.

Example 104: 9-(l-((6-Chloro-2-(l-methyl-lH-l,2,4-triazol-3-yl)pyridin-3- yl)amino)ethyl)-3-(l-(dimethylglycyl)azetidin-3-yl)-4,7-dime thyl-3,4-dihydro-5H- pyrazolo [3,4-c] isoquinolin-5-one

The title compound was prepared using similar procedures as described for Example 103 with dimethylglycine replacing 2-hydroxyacetic acid. The resulting mixture was purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5 μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C29H34CIN10O2 (M+H) ⁺ m/z = 589.2; found 589.2.

Example 105: 9-(l-((6-Chloro-2-(l-methyl-lH-l,2,4-triazol-3-yl)pyridin-3- yl)amino)ethyl)-7-methyl-4-(methyl-d3)-3-((S)-l-methylpyrrol idin-3-yl)-3,4-dihydro-5H- pyrazolo [3,4-c] isoquinolin-5-one

The title compound was prepared using similar procedures as described for Example 81 with tert-butyl (7?)-3-((methylsulfonyl)oxy)pyrrolidine-l -carboxylate replacing tert-butyl 3-((methylsulfonyl)oxy)azetidine-l-carboxylate in step 1, CD3I replacing methyl iodide in step 5, and 6-chloro-2-(l-methyl-lH-l,2,4-triazol-3-yl)pyridin-3-amine replacing 6-chloro-2- (l-methyl-lH-pyrazol-4-yl)pyridin-3-amine in step 10. The resulting mixture was purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5 μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C27H28D3CIN9O (M+H) ⁺ m/z = 535.3; found 535.2. Example 106: 9-(l-((6-Chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3-yl)ami no)ethyl)-3- ((l-(2,2-difluoroethyl)piperidin-4-yl)methyl)-4,7-dimethyl-3 ,4-dihydro-5H-pyrazolo[3,4- c]isoquinolin-5-one Step 1: benzyl 4-((9-(l-bromoethyl)-4, 7-dtmethyl-5-oxo-4,5-dthydro-3H-pyrazolo[3,4- c]isoquinolin-3-yl)methyl)piperidine-l-carboxylate

Cbz

The title compound was prepared using similar procedures as described for Example 81 with benzyl 4-(hydrazineylmethyl)piperidine-l -carboxylate replacing tert-butyl 3- hydrazineylazetidine-1 -carboxylate in step 2.

Step 2: benzyl 4-((9-(l-((6-chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3-yl )amino)ethyl)-4, 7- dimethyl-5-oxo-4,5-dihydro-3H-pyrazolo[3, 4-c]isoquinolin-3-yl)methyl)piperidine-l- carboxylate

Cbz

To benzyl 4-((9-(l-bromoethyl)-4,7-dimethyl-5-oxo-4,5-dihydro-3H-pyraz olo[3,4- c]isoquinolin-3-yl)methyl)piperidine-l -carboxylate (100 mg, 0.18 mmol) in 2 dram vial was added DMF (0.5 mb), and 6-chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3-amine (100 mg, 0.5 mmol). The mixture was heated at 80 °C for 1 h. Upon cooling to room temperature and diluted with water. The mixture was extracted with EtOAc, the combined organic layer was dried over Na2SC>4 and concentrated under vacuum. The residue was purified by silica gel column chromatography, eluted with DCM and EtOAc to provide the desired product as a brown solid (73 mg, 60%). LCMS calculated for C37H40CIN8O3 (M+H) ⁺ m/z = 679.3; found 679.3.

Step 3: 9-(l-((6-chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3-yl)ami no)ethyl)-4, 7-dimethyl- 3-(piperidin-4-ylmethyl)-3, 4-dihydro-5H-pyrazolo[3, 4-c]isoquinolin-5-one

To a mixture of benzyl 4-((9-(l-((6-chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3- yl)amino)ethyl)-4,7-dimethyl-5-oxo-4,5-dihydro-3H-pyrazolo[3 ,4-c]isoquinolin-3- yl)methyl)piperidine-l -carboxylate (73 mg, 0.11 mmol) was added Pd/C (10 wt%, 20 mg) and MeOH (4 mb). The reaction mixture was purged with hydrogen, stirred at rt for 2h. After completion, the mixture was filtered. The solvent was then removed under vacuum to afford the crude product as a pale oil, which was used directly without further purification. LCMS calculated for C29H34CIN8O (M+H) ⁺ m/z = 545.3; found 545.3.

Step 4: 9-(l-((6-chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3-yl)ami no)ethyl)-3-((l-(2,2- difluoroethyl)piperidin-4-yl)methyl)-4, 7-dimethyl-3, 4-dihydro-5H-pyrazolo[3, 4- c ] isoquinolin-5-one

To a mixture of 9-(l-((6-chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3- yl)amino)ethyl)-4,7-dimethyl-3-(piperidin-4-ylmethyl)-3,4-di hydro-5H-pyrazolo[3,4- c]isoquinolin-5-one (10 mg, 0.018 mmol), DIEA (10 uL) and MeCN (0.5 mb) was added 2,2- difluoroethyl trifluoromethane sulfonate (10 uL). The reaction mixture was heated and stirred at 60 °C for Ih. After completion, the resulting mixture was dilute with MeOH and purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5 μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid.

LCMS calculated for C ₃ IH ₃ 6C1F ₂ N ₈ O (M+H) ⁺ m/z = 609.3; found 609.2.

Examples 107-108.

Examples 107-108 in Table 12 were prepared similarly as described for Example 106.

Table 12.

Example 109: 4-((9-(l-((6-Chloro-3'-fluoro-[2,4'-bipyridin]-3-yl)amino)et hyl)-4,7- dimethyl-5-oxo-4,5-dihydro-3H-pyrazolo[3,4-c]isoquinolin-3-y l)methyl)-N- ethylpiperidine-l-carboxamide

Step 1: 9-(l-((6-chloro-3'-fluoro-[2,4'-bipyridin]-3-yl)amino)ethyl) -4, 7-dimethyl-3-(piperidin- 4-ylmethyl)-3, 4-dihydro-5H-pyrazolo[3, 4-c]isoquinolin-5-one

The title compound was prepared using similar procedures as described for Example 106 with 6-chloro-3'-fhioro-[2,4'-bipyridin]-3-amine replacing 6-chloro-2-(l -methyl- 1H- pyrazol-4-yl)pyridin-3-amine in step 2. LCMS calculated for C30H32CIFN7O (M+H) ⁺ m/z = 560.2; found 560.2.

Step 2: 4-((9-(l-((6-chloro-3'-fluoro-[2,4'-bipyridin]-3-yl)amino)et hyl)-4, 7-dimethyl-5-oxo- 4,5-dihydro-3H-pyrazolo[3,4-c]isoquinolin-3-yl)methyl)-N-eth ylpiperidine-l-carboxamide

To a mixture of 9-(l-((6-chloro-3'-fhioro-[2,4'-bipyridin]-3-yl)amino)ethyl) -4,7- dimethyl-3-(piperidin-4-ylmethyl)-3,4-dihydro-5H-pyrazolo[3, 4-c]isoquinolin-5-one (10 mg, 0.018 mmol), and MeCN (0.5 mb) was added ethyl isocyanate (10 uL). The reaction mixture was heated and stirred at 80 °C for Ih. After completion, the resulting mixture was dilute with MeOH and purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5 μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C33H37CIFN8O2 (M+H) ⁺ m/z = 631.3; found 631.2.

Example 110: 9-(l-((6-Chloro-3'-fluoro-[2,4'-bipyridin]-3-yl)amino)ethyl) -3-((l-(2,2- difluoroethyl)pyrrolidin-3-yl)methyl)-4,7-dimethyl-3,4-dihyd ro-5H-pyrazolo[3,4- c]isoquinolin-5-one

The titled compound was prepared using similar procedures as described for Example 106 with benzyl 3 -(hydrazineylmethyl)pyrrolidine-l -carboxylate replacing 4- (hydrazineylmethyl)piperidine-l -carboxylate in step 1 and 6-chloro-3'-fluoro-[2,4'-bipyridin]- 3-amine replacing 6-chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3-amine in step 2. The product was purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5 μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C31H32CIF3N7O (M+H) ⁺ m/z = 610.2; found 610.2.

Example 111: 9-(l-((6-Chloro-2-(l-methyl-lH-l,2,4-triazol-3-yl)pyridin-3- yl)amino)ethyl)-3-(l-hydroxyethyl)-4,7-dimethylimidazo[l,5-a ]quinazolin-5(4H)-one

Step 1: 9-(l-((6-chloro-2-(l-methyl-lH-l,2,4-triazol-3-yl)pyridin-3- yl)amino)ethyl)-4, 7- dimethyl-5-oxo-4, 5-dihydroimidazo [1 , 5 -a] quinazoline- 3 -carboxylic acid

The title compound was prepared using similar procedures as described for Example 4 with 6-chloro-2-(l -methyl- 1H-1, 2, 4-triazol-3-yl)pyridin -3 -amine replacing methyl 2- aminobenzoate in step 10. The crude product was used directly without further purification. LCMS calculated for C23H22CIN8O3 (M+H) ⁺ m/z = 493.1; found 493.1.

Step 2: 9-(l-((6-chloro-2-(l-methyl-lH-l,2,4-triazol-3-yl)pyridin-3- yl)amino)ethyl)-3-iodo-

4, 7-dimethylimidazo[l,5-a]quinazolin-5(4H)-one

To a mixture of 9-(l-((6-chloro-2-(l -methyl- 1H-1, 2, 4-triazol-3-yl)pyridin-3- yl)amino)ethyl)-4,7 -dimethyl-5-oxo-4,5 -dihydroimidazo[ 1 ,5 -a]quinazoline-3 -carboxylic acid (520 mg, 1.05 mmol) in DMF (5 mL) under nitrogen was added NaHCCh (441mg, 5.25 mmol) at 0 °C, after stirring for 5 min, NIS (268 mg, 1.2 mmol) was added in one portion. The resulting mixture was warmed to rt and stirred for 2h under vacuum. After completion, the reaction mixture was quenched with sat. Na2S2O ₃ and diluted with water. The crude product was collected as a brown solid by filtration which was purified by silica gel column chromatography, eluted with DCM and EtOAc to provide the desired product as a brown solid (488 mg, 81%). LCMS calculated for C22H21CIIN8O (M+H) ⁺ m/z = 575.1; found 575.1.

Step 3: 3-acetyl-9-(l-((6-chloro-2-(l-methyl-lH-l,2,4-triazol-3-yl)p yridin-3-yl)amino)ethyl)-

4, 7-dimethylimidazo[l,5-a]quinazolin-5(4H)-one

The mixture of 9-(l-((6-chloro-2-(l-methyl-lH-l,2,4-triazol-3-yl)pyridin-3- yl)amino)ethyl)-3-iodo-4,7-dimethylimidazo[l,5-a]quinazolin- 5(4H)-one (50 mg, 0.087 mmol), PdCl ₂ (PPh ₃ ) ₂ (7 mg, 0.01 mmol), and tributyl(l-ethoxyvinyl)stannane (32 mg, 0.09 mmol) in dioxane (1 mL) was heated at 100 °C for 4 h under nitrogen atmosphere. Upon cooling to room temperature, the mixture was added 2 N HC1 and stirred for 30 min before the mixture was diluted with water and extracted with EtOAc. The combined organics were washed with sat. NaCl and dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with DCM and MeOH to provide the desired product as a brown solid (32 mg, 76%). LCMS calculated for C24H24CIN8O2 (M+H) ⁺ m/z = 491.2; found 491.2. Step 4: 9-(l-((6-chloro-2-(l-methyl-lH-l,2, 4-triazol-3-yl)pyridin-3-yl)amino)ethyl)-3-(l- hydroxyethyl)-4, 7 -dimethylimidazo [1 ,5-a] ' quinazolin-5(4H)-one

To a mixture of 3-acetyl-9-(l-((6-chloro-2-(l-methyl-lH-l,2,4-triazol-3-yl)p yridin-3- yl)amino)ethyl)-4,7-dimethylimidazo[l,5-a]quinazolin-5(4H)-o ne (10 mg, 0.02 mmol), and MeOH (0.5 mL) was added NaBTU (5 mg, 0. 12 mmol) at 0 °C. The reaction mixture was stirred at same temperature for 10 min before quenched with sat. NH4CI. The resulting mixture was dilute with MeOH and purified by prep-HPLC (column: Sunfire prep Cl 8 column, 30* 150 mm, 5 μm; mobile phase A: water (0. 1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C24H26CIN8O2 (M+H) ⁺ m/z = 493.2; found 493.2.

Example 112: 9-(l-((6-Chloro-2-(l-methyl-lH-l,2,4-triazol-3-yl)pyridin-3- yl)amino)ethyl)-4,7-dimethyl-3-(pyridin-3-yl)imidazo[l,5-a]q uinazolin-5(4H)-one

The mixture of 9-(l-((6-chloro-2-(l-methyl-lH-l,2,4-triazol-3-yl)pyridin-3- yl)amino)ethyl)-3-iodo-4,7-dimethylimidazo[l,5-a]quinazolin- 5(4H)-one (Example 111, step 2) (10 mg, 0.02mmol), pyridin-3-ylboronic acid (5 mg, 0.04 moml), K3PO4 (10 mg, 0.05 mmol) and Pd(PPh3)4 (2 mg, 0.002 mmol) in dioxane (0.5 mL) and water (0. 1 mL) was heated at 100 °C for 20 min under nitrogen atmosphere. The mixture was diluted with MeOH and purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C27H25CIN9O (M+H) ⁺ m/z = 526.2; found 526.2.

Examples 113-115.

Examples 113-115 in Table 13 were prepared similarly as described for Example 112. Table 13.

Example 116: 9-(l-((6-Chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3-yl)ami no)ethyl)-

4,7-dimethyl-3-(2-methylpyridin-3-yl)imidazo[l,5-a] quinazolin-5(4H)-one

Step 1: 9-(l-((6-chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3-yl)ami no)ethyl)-4, 7-dimethyl-

5-oxo-4, 5-dihydroimidazo [1 ,5-a] quinazoline-3-carboxylic acid

The title compound was prepared using similar procedures as described for Example 4 with 6-chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3-amine (intermediate 18) replacing methyl 2 -aminobenzoate in step 10. The crude product was used directly without further purification. LCMS calculated for C24H23CIN7O3 (M+H) ⁺ m/z = 492.2; found 492.2.

Step 2: 9-(l -((6-chloro-2-(l -methyl- lH-pyrazol-4-yl)pyridin-3-yl)amino)ethyl)-3-iodo-4, 7- dimethylimidazo[l ,5 -a] quinazolin-5 ( 4H)-one

To a mixture of 9-(l-((6-chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3- yl)amino)ethyl)-4,7 -dimethyl-5-oxo-4,5 -dihydroimidazo[ 1 ,5 -a]quinazoline-3 -carboxylic acid (260 mg, 0.5 mmol) in DMF (2 mb) under nitrogen was added NaHCCE (220 mg, 2.6 mmol) at 0 °C, after stirring for 5 min, NIS (134 mg, 0.6 mmol) was added in one portion. The resulting mixture was warmed to rt and stirred for 2h under vacuum. After completion, the reaction mixture was quenched with sat. Na2S2Cf and diluted with water. The crude product was collected as a brown solid by filtration which was purified by silica gel column chromatography, eluted with DCM and EtOAc to provide the desired product as a brown solid (230 mg, 75%). LCMS calculated for C23H22CIIN7O (M+H) ⁺ m/z = 574.1; found 574.1.

Step 3: 9-(l -((6-chloro-2-(l -methyl- lH-pyrazol-4-yl)pyridin-3-yl)amino)ethyl)-4, 7-dimethyl- 3-(2-methylpyridin-3-yl)imidazo [1 ,5-a] quincizolin-5(4H)-one

The mixture of 9-(l-((6-chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3- yl)amino)ethyl)-3-iodo-4,7-dimethylimidazo[l,5-a]quinazolin- 5(4H)-one (10 mg, 0.02mmol), 2-methyl-3-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl)pyri dine (5 mg, 0.04 mol), K3PO4 (10 mg, 0.05 mmol) and Pd(PPh3)4 (2 mg, 0.002 mmol) in dioxane (0.5 mL) and water (0.1 mL) was heated at 100 °C for 20 min under nitrogen atmosphere. The mixture was diluted with MeOH and purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C29H28CIN8O (M+H) ⁺ m/z = 539.2; found 539.2.

Examples 117-118.

Examples 117-118 in Table 14 were prepared similarly as described for Example 116.

Table 14.

Example 119: 9-(l-((6-Chloro-2-(l-methyl-lH-l,2,4-triazol-3-yl)pyridin-3- yl)amino)ethyl)-N,N,4,7-tetramethyl-5-oxo-4,5-dihydroimidazo [l,5-a]quinazoline-3- carboxamide To a solution of 9-(l-((6-chloro-2-(l -methyl- 1H-1, 2, 4-triazol-3-yl)pyridin-3- yl)amino)ethyl)-4,7 -dimethyl-5-oxo-4,5 -dihydroimidazo[ 1 ,5 -a]quinazoline-3 -carboxylic acid (Example 111, step 1) (20 mg, 0.04 mmol) in DMF (0.5 ml) was added propanephosphonic acid anhydride (50% THF solution, 32 μL, 0.05 mmol) and DIEA (20 μL), and dimethylamine (2M THF solution, 40 μL) at 0 °C. The resulting mixture was warmed to rt and stirred for 5h. The mixture was diluted MeOH and purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5μm; mobile phase A: water (0. 1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a yellow solid. LCMS calculated for C25H27CIN9O2 (M+H) ⁺ m/z = 520.2; found 520.2.

Example 120: 9-(l-((6-Chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3-yl)ami no)ethyl)- N,N,4,7-tetramethyl-5-oxo-4,5-dihydroimidazo[l,5-a]quinazoli ne-3-carboxamide

To a solution of 9-(l-((6-chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3- yl)amino)ethyl)-4,7 -dimethyl-5-oxo-4,5 -dihydroimidazof 1 ,5 -a]quinazoline-3 -carboxylic acid (Example 116, step 1) (20 mg, 0.04 mmol) in DMF (0.5 ml) was added propanephosphonic acid anhydride (50% THF solution, 32 μL, 0.05 mmol) and DIEA (20 μL), and dimethylamine (2M THF solution, 40 μL) at 0 °C. The resulting mixture was warmed to rt and stirred for 5h. The mixture was diluted MeOH and purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5μm; mobile phase A: water (0. 1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a yellow solid. LCMS calculated for C26H ₂₈ C1N ₈ O2 (M+H) ⁺ m/z = 519.2; found 519.2.

Examples 121-122.

Examples 121-122 in Table 15 were prepared similarly as described for Example 120.

Table 15.

Example 123: 9-(l-((6-chloro-2-(l-methyl-lH-l,2,4-triazol-3-yl)pyridin-3- yl)amino)ethyl)-4-(2,2-difluoroethyl)-N,N,7-trimethyl-5-oxo- 4,5-dihydroimidazo[l,5- a] quinazoline-3-carboxamide

Step 1: 9-(l-((6-chloro-2-(l-methyl-lH-l,2,4-triazol-3-yl)pyridin-3- yl)amino)ethyl)-4-(2,2- difluoroethyl)-7-methyl-5-oxo-4,5-dihydroimidazo[l,5-a]quina zoline-3-carboxylic acid

The title compound was prepared using similar procedures as described for Example 4 with 2,2-difluoroethan-l -amine replacing methylamine in step 3, and 6-chloro-2-( 1 -methyl - lH-l,2,4-triazol-3-yl)pyridin-3-amine replacing methyl 2 -aminobenzoate in step 10. The crude product was used directly without further purification. LCMS calculated for C ₂₄ H ₂₂ C1F ₂ N ₈ O ₃ (M+H) ⁺ m/z = 543.1; found 543.1.

Step 2: 9-(l-((6-chloro-2-(l-methyl-lH-l,2,4-triazol-3-yl)pyridin-3- yl)amino)ethyl)-4-(2,2- di fluoroethyl) -N,N, 7-trimethyl-5-oxo-4,5-dihydroimidazo [1 ,5-a] quinazoline-3-carboxamide

To a solution of 9-(l-((6-chloro-2-(l-methyl-lH-l,2,4-triazol-3-yl)pyridin-3- yl)amino)ethyl)-4-(2,2-difluoroethyl)-7-methyl-5-oxo-4,5-dih ydroimidazo[l,5-a]quinazoline- 3-carboxylic acid (20 mg, 0.04 mmol) in DMF (0.5 ml) was added propanephosphonic acid anhydride (50% THF solution, 32 μL, 0.05 mmol) and DIEA (20 μL). and dimethylamine (2M THF solution, 40 μL) at 0 °C. The resulting mixture was warmed to rt and stirred for 5h. The mixture was diluted MeOH and purified by prep-HPLC (column: Sunfire prep Cl 8 column, 30* 150 mm, 5μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a yellow solid. LCMS calculated for C ₂ 6H ₂ 7ClF ₂ NgO ₂ (M+H) ⁺ m/z = 570.2; found 570.2.

Example 124: 9-(l-((6-Chloro-2-(3-methyl-lH-pyrazol-4-yl)pyridin-3-yl)ami no)ethyl)-

N,N,4,7-tetramethyl-5-oxo-4,5-dihydroimidazo[l,5-a]quinaz oline-3-carboxamide

Step 1: 9-acetyl-N,N,4, 7-tetramethyl-5-oxo-4,5-dihydroimidazo [1 ,5-a] ' quinazoline-3- carhoxamide tert-Butyl 9-acetyl-4,7-dimethyl-5-oxo-4,5-dihydroimidazo[l,5-a]quinazo line-3- carboxylate (Example 4, Step 7) (1.0 g, 2.8 mmol) was dissolved in TFA (5 mL). The mixture was stirred for 3 h at rt. After completion, TFA was removed under vacuum. 50 mL of ice water and 20 mL of EtOAc were added to the reaction residue while stirring. After stirring for 20 min, the resulting solid was collected to afford a light brown solid, which was washed with water (2X). The solid was dried under vacuum to provide a beige solid. The solid product was dissolved in DMF (10 mL), then was added propanephosphonic acid anhydride (50% THF solution, 1.9 mL, 0.05 mmol) and DIEA (522 μL, 3 mmoL), and dimethylamine (2M THF solution, 1.5 mL) at 0 °C. The resulting mixture was warmed to rt and stirred for 5h. The mixture was diluted with water and extracted with DCM/IPA 3: 1. The combined organic layers were washed with sat. NaCl, dried with anhydrous sodium sulfate, fdtered, and concentrated under reduced pressure which was used directly in the next step. LCMS calculated for C17H19N4O3 (M+H) ⁺ m/z = 327.1; found 327.2.

Step 2: 9-(l -hydroxyethyl)-N,N,4, 7-tetramethyl-5-oxo-4,5-dihydroimidazo[l,5-a]quinazoline- 3-carboxamide

9-Acetyl -N,N,4, 7 -tetramethyl -5 -oxo -4,5 -dihydroimidazo [ 1 , 5 -a] quinazoline-3 - carboxamide (500 mg, 1.53 mmol) was dissolved in MeOH (10 mL) and DCM (10 mL) then NaBHt (60 mg, 1.6 mmol) was added at 0 °C in three portions under nitrogen. The resulting mixture was stirred at same temperature for 10 min before quenched with sat. NH4CI. The mixture was diluted with water and extracted with DCM. The combined organics were washed with sat. NaCl, and dried with anhydrous sodium sulfate, fdtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with DCM and MeOH (0 to 4%) to provide the desired product as a light yellow solid (378 mg, 77%). LCMS calculated for C17H21N4O3 (M+H) ⁺ m/z = 329.2; found 329.2.

Step 3: 9-(l-bromoethyl)-N,N,4, 7-tetramethyl-5-oxo-4,5-dihydroimidazo[l,5-a]quinazoline-3- carboxamide

To a mixture of 9-(l-hydroxyethyl)-N,N,4,7-tetramethyl-5-oxo-4,5- dihydroimidazo[l,5-a]quinazoline-3-carboxamide (300 mg, 0.91 mmol) in DCM (200 mL) was added PBr3 (114 uL, 1.2 mmol) at 0 °C. The resulting mixture was warmed to rt and stirred for 2 h. The mixture was then quenched with sat. NaHCCE and extracted with DCM (2x). The combined organics were washed with sat. NaCl, and dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was used directly in the next step without further purification.

Step 4: 9-(l-((6-chloro-2-(3-methyl-lH-pyrazol-4-yl)pyridin-3-yl)ami no)ethyl)-N,N,4, 7- tetramethyl-5-oxo-4,5-dihydroimidazo[l,5-a]quinazoline-3-car boxamide

To the solution of 9-(l-bromoethyl)-N,N,4,7-tetramethyl-5-oxo-4,5- dihydroimidazo[l,5-a]quinazoline-3-carboxamide (10 mg, 0.025 mmol) in DMF (0.2 mL) was added tert-butyl 4-(3-amino-6-chloropyridin-2-yl)-3-methyl-lH-pyrazole-l-carb oxylate (intermediate 22) (20 mg, 0.06 mmol). The mixture was heated to 80 °C for 2 h. Upon cooling to room temperature, the mixture was added TFA (0.5 mL) and stirred for 30 min, then diluted with MeOH and purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C26H28CIN8O2 (M+H) ⁺ m/z = 519.2; found 519.2.

Examples 125-130.

Examples 125-130 in Table 16 were prepared similarly as described for Example 124.

Table 16.

Example 131: 9-(l-((6-Chloro-2-(l-methyl-lH-l,2,4-triazol-3-yl)pyridin-3- yl)amino)ethyl)-4,7-dimethyl-3-(l-methylpiperidin-4-yl)imida zo[l,5-a] quinazolin-5(4/T)- one Step 1: tert-butyl 4-(9-acetyl-4, 7-dimethyl-5-oxo-4,5-dihydroimidazo[l ,5-a] quinazolin-3-yl)- 3, 6-dihydropyridine-l (2H) -carboxylate

A mixture of 9-acetyl-3-iodo-4,7-dimethylimidazo[l,5-a]quinazolin-5(4H)-o ne (Example 71, step 1) (1.0 g, 2.6 mmol), PdXPhosG2 (156 mg, 0.2 mmol), tert-butyl 4- (4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl)-3,6-dihydropyr idine-l(2H)-carboxylate (927 mg, 3 mmol) and K3PO4 (636 mg, 3 mmol) in dioxane (20 mL) and water (4 mL) was heated at 100 °C for 2 h under nitrogen atmosphere. Upon cooling to room temperature, the mixture was diluted with water and extracted with EtOAc. The combined organic layers were washed with sat. NaCl, dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with DCM and ethyl acetate to provide the desired product as a brown solid (829 mg, 73%). LCMS calculated for C24H29N4O4 (M+H) ⁺ m/z = 437.2; found 437.2.

Step 2: tert-butyl 4-(9-(l-hydroxyethyl)-4, 7-dimethyl-5-oxo-4,5-dihydroimidazo [1 ,5- a]quinazolin-3-yl)-3, 6-dihydropyridine-l (2H) -carboxylate

To a mixture of tert-butyl 4-(9-acetyl-4,7-dimethyl-5-oxo-4,5-dihydroimidazo[l,5- a]quinazolin-3-yl)-3,6-dihydropyridine-l(2H)-carboxylate (829 mg, 1.9 mmol) in MeOH (30 mL) and DCM (30 mL) was added NaBEL (114 mg, 3 mmol) at 0 °C. The resulting mixture was stirred at same temperature for 10 min before being quenched with sat. NH4CI. The mixture was diluted with water and extracted with DCM/IPA 3: 1. The combined organic layers were washed with sat. NaCl and dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with DCM and MeOH to provide the desired product as a white solid (757 mg, 91%). LCMS calculated for C24H31N4O4 (M+H) ⁺ m/z = 439.2; found 439.2.

Step 3: tert-butyl 4-(9-(l-hydroxyethyl)-4, 7-dimethyl-5-oxo-4,5-dihydroimidazo[l,5- a]quinazolin-3-yl)piperidine-l-carboxylate

To a mixture of tert-butyl 4-(9-(l-hydroxyethyl)-4,7-dimethyl-5-oxo-4,5- dihydroimidazo[l,5-a]quinazolin-3-yl)-3,6-dihydropyridine-l( 2H)-carboxylate (757 mg, 1.7 mmol) in MeOH (20 mL) was added ammonium formate (630 mg, 10 mmol), Pd/C (10 wt%, 185 mg, 0.17 mmol) and acetic acid (0.5 mL) at rt under nitrogen atmosphere. The resulting mixture was stirred at 50 °C for 10 h. The mixture was fdtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with DCM and MeOH to provide the desired product as a white solid (751 mg, 86%). LCMS calculated for C24H33N4O4 (M+H) ⁺ m/z = 441.2; found 441.2.

Step 4: tert-butyl 4-(9-(l-bromoethyl)-4, 7-dtmethyl-5-oxo-4,5-dthydrotmtdazo[l,5- a]quinazolin-3-yl)piperidine-l-carboxylate

To a mixture of tert-butyl 4-(9-(l-hydroxyethyl)-4,7-dimethyl-5-oxo-4,5- dihydroimidazo[l,5-a]quinazolin-3-yl)piperidine-l-carboxylat e (300 mg, 0.68 mmol) in THF (20 mL) was added pyridine (0.22 mL, 2.72 mmol), PBr3 (367 mg, 1.38 mmol). The resulting mixture was stirred at rt for 2 h. The mixture was then quenched with sat. NaHCO; and extracted with EtOAc. The combined organic layers were washed with sat. NaCl, dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was used directly in the next step without further purification.

Step 5: 9-(l-((6-chloro-2-(l-methyl-lH-l,2,4-triazol-3-yl)pyridin-3- yl)amino)ethyl)-4, 7- dimethyl-3-(piperidin-4-yl)imidazo[l,5-a]quinazolin-5(4H)-on e tert-Butyl 4-(9-( 1 -bromoethyl)-4,7 -dimethyl-5 -oxo-4,5 -dihydroimidazo [1,5- a]quinazolin-3-yl)piperidine-l-carboxylate (60 mg, 0.12 mmol) in 2 dram vial was added DMF (0.3 mL), and 6-chloro-2-(l-methyl-lH-l,2,4-triazol-3-yl)pyridin-3-amine (60 mg, 0.30 mmol). The mixture was heated to 80 °C for 1 h. Upon cooling to room temperature, to the mixture was added TFA (0.5 mL) and stirred for 30 min before diluted with MeOH and purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C27H31CIN9O (M+H) ⁺ m/z = 532.2; found 532.2.

Step 6: 9-(l-((6-chloro-2-(l-methyl-lH-l,2,4-triazol-3-yl)pyridin-3- yl)amino)ethyl)-4, 7- dimethyl-3-(l-methylpiperidin-4-yl)imidazo[l,5-a]quinazolin- 5(4H)-one

To a mixture of 9-(l-((6-chloro-2-(l -methyl- 1H-1, 2, 4-triazol-3-yl)pyridin-3- yl)amino)ethyl)-4,7-dimethyl-3-(piperidin-4-yl)imidazo[l,5-a ]quinazolin-5(4H)-one (10 mg, 0.02 mmol) was added DCM (0.5 mL), formaldehyde (water solution, 10 uL) and sodium triacetoxyborohydride (10 mg, 0.04 mmol). The mixture was stirred for 30 min, then diluted with MeOH and purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C28H33CIN9O (M+H) ⁺ m/z = 546.2; found 546.2.

Example 132: 3-(l-Acetylpiperidin-4-yl)-9-(l-((6-chloro-2-(l-methyl-lH-l, 2,4-triazol-3- yl)pyridin-3-yl)amino)ethyl)-4,7-dimethylimidazo[l,5-a]quina zolin-5(4H)-one

To a mixture of 9-(l-((6-chloro-2-(l -methyl- 1H-1, 2, 4-triazol-3-yl)pyridin-3- yl)amino)ethyl)-4,7-dimethyl-3-(piperidin-4-yl)imidazo[l,5-a ]quinazolin-5(4H)-one (Example 131, step 5) (10 mg, 0.019 mmol) in DCM (0.5 mL) was added acetyl chloride (3.2 mg, 0.04 mmol) DCM (0.2 mL) solution and A.A-diisopropylcthylaminc (5.0 uL) at 0 °C. The reaction mixture was stirred at same temperature for 30 minutes before the solvent was removed under vacuum. The resulting mixture was diluted with methanol and purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5 μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C29H33CIN9O2 (M+H) ⁺ m/z = 574.2; found 574.2.

Example 133.

Example 133 in Table 17 were prepared similarly as described for Example 132.

Table 17.

Example 134: 9-(l-((6-Chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3-yl)ami no)ethyl)-

4,7-dimethyl-3-(l-methylpiperidin-4-yl)imidazo[l,5-a] quinazolin-5(4H)-one

The titled compound was prepared using similar procedures as described for Example 131 with 6-chloro-2-(l -methyl- lH-pyrazol-4-yl)pyridin-3 -amine replacing 6-chloro-2-(l- methyl-lH-l,2,4-triazol-3-yl)pyridin-3-amine in step 5. The crude product was used directly without further purification. LCMS calculated for C29H34CIN8O (M+H) ⁺ m/z = 545.3; found 545.2.

Example 135: 9-(l-((6-Chloro-2-(l-methyl-LH-pyrazol-4-yl)pyridin-3-yl)ami no)ethyl)-

4,7-dimethyl-3-(l-methylpyrrolidin-3-yl)-3,4-dihydro-5/f- pyrazolo[3,4-c]isoquinolin-5- one

Step 1: benzyl 3-(9-(l-bromoethyl)-4, 7-dtmethyl-5-oxo-4,5-dthydro-3H-pyrazolo[3,4- c]isoquinolin-3-yl)pyrrolidine-l-carboxylate

The titled compound was prepared using similar procedures as described for Example 81 with phenylmethyl 3-[(methylsulfonyl)oxy]-l-pyrrolidinecarboxylate replacing tert-butyl 3-((methylsulfonyl)oxy)azetidine-l -carboxylate in Step 1.

Step 2: 9-(l -((6-chloro-2-(l -methyl- lH-pyrazol-4-yl)pyridin-3-yl)amino)ethyl)-4, 7-dimethyl- 3-(pyrrolidin-3-yl)-3, 4-dihydro-5H-pyrazolo[3,4-c]isoquinolin-5-one

Benzyl 3-(9-( 1 -bromoethyl)-4,7-dimethyl-5-oxo-4,5-dihydro-3H-pyrazolo[3,4- c]isoquinolin-3-yl)pyrrolidine-l -carboxylate (58 mg, 0.11 mmol) in 2 dram vial was added DMF (0.2 mL), and 6-chloro-2-( 1 -methyl- lH-pyrazol-4-yl)pyridin-3 -amine (20 mg, 0.096 mmol). The mixture was heated at 80 °C for 1 h. Upon cooling to room temperature, the mixture was added HBr (48 wt. % in acetic acid, 1 mL) and stirred for 30 min at 0 °C. The resulting mixture was diluted with MeOH and purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C27H30CIN8O (M+H) ⁺ m/z = 517.2; found 517.2.

Step 3: 9-(l -((6-chloro-2-(l -methyl- lH-pyrazol-4-yl)pyridin-3-yl)amino)ethyl)-4, 7-dimethyl- 3-(l-methylpyrrolidin-3-yl)-3, 4-dihydro-5H-pyrazolo[3, 4-c]isoquinolin-5-one

To a mixture of 9-(l-((6-chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3- yl)amino)ethyl)-4,7-dimethyl-3-(pyrrolidin-3-yl)-3,4-dihydro -5H-pyrazolo[3,4-c]isoquinolin- 5-one (5 mg, 0.01 mmol) was added DCM (0.5 mL), formaldehyde (water solution, 10 μL) and sodium triacetoxyborohydride (4.2 mg, 0.02 mmol). The mixture was stirred for 30 min, then diluted with MeOH and purified by prep-HPLC (column: Sunfire prep Cl 8 column, 30* 150 mm, 5μm; mobile phase A: water (0. 1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C28H32CIN8O (M+H) ⁺ m/z = 531.2; found 531.2.

Examples 136-141.

Examples 136-141 in Table 18 were prepared similarly as described for Example 135. Table 18.

Example 142: 9-(l-((6-Chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3-yl)ami no)ethyl)-3- (l-(2,2-difluoroethyl)pyrrolidin-3-yl)-4,7-dimethyl-3,4-dihy dro-5H-pyrazolo[3,4- c]isoquinolin-5-one

To a solution of 9-(l-((6-chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3- yl)amino)ethyl)-4,7-dimethyl-3-(pyrrolidin-3-yl)-3,4-dihydro -5H-pyrazolo[3,4-c]isoquinolin- 5-one (Example 135, step 2) (5 mg, 0.01 mmol) in DMF (0.2 mb) was added 2,2- difluoroethyl trifluoromethane sulfonate (10.4 mg, 0.05 mmol) and A.A-diisopropylcthylaminc (6.3 mg, 0.05 mmol). The reaction was stirred at room temperature for 16 hours. The resulting mixture was diluted with methanol and purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5 μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C29H32CIF2N8O (M+H) ⁺ m/z = 581.2; found 581.2.

Examples 143-144.

Examples 143-144 in Table 19 were prepared similarly as described for Example 142. Table 19.

Example 145: 9-(l-((6-Chloro-2-(l-methyl-LH-l,2,4-triazol-3-yl)pyridin-3- yl)amino)ethyl)-4-ethyl-3-(l-(2-hydroxyethyl)pyrrolidin-3-yl )-7-methyl-3,4-dihydro-5/f- pyrazolo [3,4-c] isoquinolin-5-one

Step 1: 9-(l-((6-chloro-2-(l-methyl-lH-l,2,4-triazol-3-yl)pyridin-3- yl)amino)ethyl)-4-ethyl-7- methyl-3-(pyrrolidin-3-yl)-3, 4-dihydro-5H-pyrazolo[3,4-c]isoquinolin-5-one The titled compound was prepared using similar procedures as described for Example 81 with phenylmethyl 3-[(methylsulfonyl)oxy]-l-pyrrolidinecarboxylate replacing tert-butyl 3-((methylsulfonyl)oxy)azetidine-l-carboxylate in Step 1, iodoethane replacing iodomethane in step 5, and 6-chloro-2-(l -methyl- 1H-1, 2, 4-triazol-3-yl)pyridin-3 -amine replacing 6-chloro- 2-(l -methyl- lH-pyrazol-4-yl)pyridin -3 -amine in Step 10. The crude product was purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5 μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C27H31CIN9O (M+H) ⁺ m/z = 532.2; found 532.2.

Step 2: 9-(l-((6-chloro-2-(l-methyl-lH-l,2,4-triazol-3-yl)pyridin-3- yl)amino)ethyl)-4-ethyl-3- (l-(2-hydroxyethyl)pyrrolidin-3-yl)-7-methyl-3, 4-dihydro-5H-pyrazolo[3, 4-c]isoquinolin-5- one

To a mixture of 9-(l-((6-chloro-2-(l-methyl-lH-l,2,4-triazol-3-yl)pyridin-3- yl)amino)ethyl)-4-ethyl-7-methyl-3-(pyrrolidin-3-yl)-3,4-dih ydro-5H-pyrazolo[3,4- c]isoquinolin-5-one (7 mg, 0.01 mmol) was added DCM (0.5 mb), 2-((tert- butyldimethylsilyl)oxy)acetaldehyde (10 mg, 0.05 mmol) and sodium triacetoxyborohydride (5.6 mg, 0.03 mmol). The mixture was stirred for 30 min, solvent was removed under vacuum. 4 M HC1 in dioxane (0.3 mL) and MeOH (0.2 mL) were added and the mixture was stirred for 10 min before being diluted with MeOH and purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5μm; mobile phase A: water (0. 1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C29H35CIN9O2 (M+H) ⁺ m/z = 576.2; found 576.2.

Examples 146-147.

Examples 146-147 in Table 20 were prepared similarly as described for Example 145.

Table 20.

Example 148: 9-(l-((6-Chloro-2-(l-methyl-lH-l,2,4-triazol-3-yl)pyridin-3- yl)amino)ethyl)-4-ethyl-7-methyl-3-(l-(methylsulfonyl)pyrrol idin-3-yl)-3,4-dihydro-5H- pyrazolo [3,4-c] isoquinolin-5-one

To a solution of 9-(l-((6-chloro-2-(l-methyl-lH-l,2,4-triazol-3-yl)pyridin-3- yl)amino)ethyl)-4-ethyl-7-methyl-3-(pyrrolidin-3-yl)-3,4-dih ydro-5H-pyrazolo[3,4- c]isoquinolin-5-one (Example 145, step 1) (5.0 mg, 0.01 mmol) in DCM (0.5 mb) was added methanesulfonyl chloride (4.0 mg, 0.04 mmol) and A.A-diisopropylcthylaminc (2.4 mg, 0.02 mmol). The reaction mixture was stirred at room temperature for 30 minutes before the solvent was removed under vacuum. The resulting mixture was diluted with methanol and purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5 μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid.

LCMS calculated for C28H33CIN9O3S (M+H) ⁺ m/z = 610.2; found 610.2.

Examples 149-151.

Examples 149-151 in Table 21 were prepared similarly as described for Example 148.

Table 21.

Example 152: 3-(3-(9-(l-((6-Chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3- yl)amino)ethyl)-7-methyl-4-(methyl-d3)-5-oxo-4,5-dihydro-3H- pyrazolo[3,4- c]isoquinolin-3-yl)pyrrolidin-l-yl)propanenitrile Step 1: 9-(l -((6-chloro-2-(l -methyl- lH-pyrazol-4-yl)pyridin-3-yl)amino)ethyl)-7-methyl-4-

(methyl-d3)-3-(pyrrolidin-3-yl)-3, 4-dihydro-5H-pyrazolo[3,4-c]isoquinolin-5-one

The titled compound was prepared using similar procedures as described for Example 81 with phenylmethyl 3-[(methylsulfonyl)oxy]-l-pyrrolidinecarboxylate replacing tert-butyl 3-((methylsulfonyl)oxy)azetidine-l-carboxylate in Step 1, iodomethane -di replacing iodomethane in step 5. The crude product was deprotected with neat 30% HBr AcOH solution and purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5 μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C27H27D3CIN8O (M+H) ⁺ m/z = 520.2; found 520.2.

Step 2: 3-(3-(9-(l-((6-chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3- yl)amino)ethyl)-7- methyl-4-(methyl-d3)-5-oxo-4,5-dihydro-3H-pyrazolo[3, 4-c]isoquinolin-3-yl)pyrrolidin-l- yl)propanenitrile

To a solution of 9-(l-((6-chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3- yl)amino)ethyl)-7-methyl-4-(methyl-d3)-3-(pyrrolidin-3-yl)-3 ,4-dihydro-5H-pyrazolo[3,4- c]isoquinolin-5-one (10 mg, 0.02 mmol) in methanol (0.5 mb) was added acrylonitrile (5.1 mg, 0.1 mmol) and triethylamine (9.7 mg, 0.1 mmol). The reaction mixture was stirred at 50 °C for 6 hours. Upon cooling to room temperature, the resulting mixture was diluted with methanol and purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C30H30D3CIN9O (M+H) ⁺ m/z = 573.2; found 573.2.

Example 153.

Example 153 in Table 22 were prepared similarly as described for Example 152. Table 22.

Example 154: 9-(l-((6-Chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3-yl)ami no)ethyl)-3- (l-(2-hydroxyacetyl)pyrrolidin-3-yl)-7-methyl-4-(methyl-J3)- 3,4-dihydro-5/f- pyrazolo [3,4-c] isoquinolin-5-one

To a solution of 9-(l-((6-chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3- yl)amino)ethyl)-7-methyl-4-(methyl-d3)-3-(pyrrolidin-3-yl)-3 ,4-dihydro-5H-pyrazolo[3,4- c]isoquinolin-5-one (Example 152, step 1) (10 mg, 0.02 mmol) in DMF (0.2 mb) was added 2-hydroxyacetic acid (2.9 mg, 0.04 mmol), HATU (14.6 mg, 0.04 mmol) and N,N- diisopropylethylamine (7.5 mg, 0.06). The reaction mixture was stirred at room temperature for 30 minutes. The resulting mixture was diluted with methanol and purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C29H ₂ 9D ₃ C1N ₈ O3 (M+H) ⁺ m/z = 578.2; found 578.2.

Examples 155-157.

Examples 155-157 in Table 23 were prepared similarly as described for Example 154. Table 23.

Example 158: 9-(l-((6-Chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3-yl)ami no)ethyl)- 4,7-dimethyl-3-(l-methylpiperidin-4-yl)-3,4-dihydro-5H-pyraz olo[3,4-c]isoquinolin-5- one

Step 1: benzyl 4-[9-(l-bromoethyl)-4, 7-dimethyl-5-oxo-pyrazolo[3,4-c]isoquinolin-3- yl ] piperidine- 1 -carboxylate

The titled compound was prepared using similar procedures as described for Example 81 with benzyl 4-hydrazinopiperidine-l -carboxylate replacing tert-butyl 3- hydrazineylazetidine-1 -carboxylate in Step 2. The resulting residue was used directly in next step without further purification.

Step 2: benzyl 4-[9-[l-[[6-chloro-2-(l-methylpyrazol-4-yl)-3-pyridyl] amino] ethyl] -4, 7- dimethyl-5-oxo-pyrazolo[3,4-c]isoquinolin-3-yl]piperidine-l- carboxylate

To a solution of benzyl 4-[9-(l-bromoethyl)-4,7-dimethyl-5-oxo-pyrazolo[3,4- c]isoquinolin-3-yl]piperidine-l -carboxylate (83.0 mg, 0.15 mmol) in 0.5 mb DMF was added 6-chloro-2-(l-methylpyrazol-4-yl)pyridin-3-amine (38.7 mg, 0.19 mmol) The resulting solution was heated to 60 °C for 3 h. Upon cooling down to r.t., the mixture was concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with ethyl acetate in dichloromethane (0 to 100%) to afford the desired product. LCMS calculated for CseFEsClNgCE (M+H) ⁺ m/z = 665.3 Found 665.3.

Step 3: 9-[l-[[6-chloro-2-(l-methylpyrazol-4-yl)-3-pyridyl] amino] ethyl] -4, 7-dimethyl-3-(4- piperidyl)pyrazolo[3, 4-c]isoquinolin-5-one

At 0 °C, to a 2 dram vial containing benzyl 4-[9-[l-[[6-chloro-2-(l-methylpyrazol-4- yl)-3-pyridyl]amino]ethyl]-4,7-dimethyl-5-oxo-pyrazolo[3,4-c ]isoquinolin-3-yl]piperidine-l- carboxylate (18.0 mg, 0.03 mmol) was added 0.5 mL hydrogen bromide solution (33 wt. % in acetic acid). The resulting solution was stirred at the same temperature for 30 min, then diluted with MeOH and purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C28H32CIN8O (M+H) ⁺ m/z = 531.2; found 531.2.

Step 4: 9-(l -((6-chloro-2-(l -methyl- lH-pyrazol-4-yl)pyridin-3-yl)amino)ethyl)-4, 7-dimethyl- 3-(l-methylpiperidin-4-yl)-3, 4-dihydro-5H-pyrazolo[3, 4-c]isoquinolin-5-one

To a solution of 9-[l-[[6-chloro-2-(l-methylpyrazol-4-yl)-3-pyridyl]amino]eth yl]- 4,7-dimethyl-3-(4-piperidyl)pyrazolo[3,4-c]isoquinolin-5-one (5.0 mg , 2.8 umol) in 1 mL DCM was added formaldehyde (1.05 uL, 14.10 umol), followed by sodium triacetoxyborohydride (1.8 mg, 8.46 umol). The resulting solution was stirred at r.t. for Ih, then concentrated under vacuum. The crude product was diluted with MeOH and purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C29H34CIN8O (M+H) ⁺ m/z = 545.3, found 545.2.

Examples 159-171.

Examples 159-171 in Table 24 were prepared similarly as described for Example 158.

Table 24.

Example 172: 9- [1- [ [6-Chloro-2-(l-methylpyrazol-4-yl)-3-pyridyl] amino] ethyl] -3- [l-(2- hydroxy-2-methyl-propyl)-4-piperidyl]-4,7-dimethyl-pyrazolo[ 3,4-c]isoquinolin-5-one To a solution of 9-[l-[[6-chloro-2-(l-methylpyrazol-4-yl)-3-pyridyl]amino]eth yl]- 4,7-dimethyl-3-(4-piperidyl)pyrazolo[3,4-c]isoquinolin-5-one (Example 158, step 3) (5.0 mg, 0.01 mmol) in 0.5 mL EtOH was added 2,2-dimethyloxirane (8 μL, 0.09 mmol), the resulting solution was heated to 80 °C for 30 min. Upon cooling down to r.t. The mixture was diluted with MeOH and purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C32H40CIN8O2 (M+H) ⁺ m/z = 603.3; found 603.2.

Examples 173-175.

Examples 173-175 in Table 25 were prepared similarly as described for Example 172.

Table 25. Example 176: 9-(l-((6-Chloro-2-(l-methyl-lH-l,2,4-triazol-3-yl)pyridin-3- yl)amino)ethyl)-4,7-dimethyl-3-(piperidin-4-yl)-3,4-dihydro- 5H-pyrazolo[3,4- c]isoquinolin-5-one

The titled compound was prepared using similar procedures as described for Example 158 with 6-chloro-2-(l -methyl- 1H-1, 2, 4-triazol-3-yl)pyridin -3 -amine replacing 6-chloro-2- (l-methyl-lH-pyrazol-4-yl)pyridin-3-amine in Step 2. The crude product was purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C27H31CIN9O (M+H) ⁺ m/z = 532.2; found 532.2

Example 177: 9-[l-[[6-Chloro-2-(l-methyl-l,2,4-triazol-3-yl)-3-pyridyl]am ino]ethyl]-3-

[l-(2-hydroxyethyl)-4-piperidyl]-4,7-dimethyl-pyrazolo[3, 4-c]isoquinolin-5-one

To a solution of 9-[l-[[6-chloro-2-(l-methyl-l,2,4-triazol-3-yl)-3- pyridyl]amino]ethyl]-4,7-dimethyl-3-(4-piperidyl)pyrazolo[3, 4-c]isoquinolin-5-one (5.0 mg , 0.01 mmol) in 1 ml DMF was added was added 2-[tert-butyl(dimethyl)silyl]oxyacetaldehyde (4.9 mg, 0.03 mmol), followed by sodium triacetoxyborohydride (5 mg, 0.023mmol). The mixture was stirred for 30 min, solvent was removed under vacuum. 4 M HC1 in dioxane (0.3 mL) and MeOH (0.2 mL) was added and the mixture was stirred for 10 min before diluted with MeOH and purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C29H35CIN9O2 (M+H) ⁺ m/z = 576.3; found page 576.2.

Examples 178-179.

Examples 178-179 in Table 26 were prepared similarly as described for Example 177. Table 26.

Example 180: 3-[4-[9-[l-[[6-Chloro-2-(l-methyl-l,2,4-triazol-3-yl)-3- pyridyl] amino] ethyl] -4,7-dimethyl-5-oxo-pyrazolo [3,4-c] isoquinolin-3-yl] -1-

The titled compound was prepared using similar procedures as described for Example 152 with 9-( 1 -((6-chloro-2-( 1 -methyl- 1H- 1 ,2,4-triazol-3 -yl)pyridin-3 -yl)amino)ethyl)-4,7 - dimethyl-3-(piperidin-4-yl)-3,4-dihydro-5H-pyrazolo[3,4-c]is oquinolin-5-one replacing 9-(l- ((6-chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3-yl)amino)et hyl)-7-methyl-4-(methyl-d3)-3- (pyrrolidin-3-yl)-3,4-dihydro-5H-pyrazolo[3,4-c]isoquinolin- 5-one in step 2. The crude product was purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C30H34CIN10O (M+H) ⁺ m/z = 585.2; found 585.2. Example 181: 9-(l-((6-Chloro-2-(l-methyl-lH-l,2,4-triazol-3-yl)pyridin-3- yl)amino)ethyl)-3-(l-(2,2-difluoroethyl)piperidin-4-yl)-4,7- dimethyl-3,4-dihydro-5H- pyrazolo [3,4-c] isoquinolin-5-one

The titled compound was prepared using similar procedures as described for Example 142 with 9-( 1 -((6-chloro-2-( 1 -methyl- 1H- 1 ,2,4-triazol-3 -yl)pyridin-3 -yl)amino)ethyl)-4,7 - dimethyl-3 -(piperidin-4-yl)-3 ,4-dihydro-5H-pyrazolo [3 ,4-c]isoquinolin-5 -one (Example 176) replacing 9-( 1 -((6-chloro-2-( 1 -methyl- 1 H-py razol -4-yl )py ridi n-3 -yl)amino)ethyl)-4,7- dimethyl-3 -(pyrrolidin-3 -yl) -3 ,4-dihydro-5//-py razolo [3 ,4-c]isoquinolin-5 -one . The crude product was purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C29H33CIF2N9O (M+H) ⁺ m/z = 596.2; found 596.2.

Example 182: 3-(l-Acetylpiperidin-4-yl)-9-(l-((6-chloro-2-(l-methyl-lH-py razol-4- yl)pyridin-3-yl)amino)ethyl)-4,7-dimethyl-3,4-dihydro-5H-pyr azolo[3,4-c]isoquinolin-5- one

At 0 °C, to a solution of 9-[l-[[6-chloro-2-(l-methylpyrazol-4-yl)-3- pyridyl] amino] ethyl] -4,7-dimethyl-3 -(4-piperidyl)pyrazolo [3 ,4-c]isoquinolin-5 -one (Example 158, step 3) (10.0 mg, 0.02 mmol) in 1ml DCM was added /V-ethyl-A-isopropyl-propan-2- amine (9 uL, 0.06 mmol). Then acetyl chloride (2 uL, 0.03 mmol) dissolved in 0.5 mb DCM was added dropwise. The resulting solution was stirred at 0 °C for 15 min and then concentrated under vacuum. The crude product was diluted with MeOH and purified by prep- HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C30H34CIN8O2 (M+H) ⁺ m/z = 573.3; found 573.3.

Examples 183-192. Examples 183-192 in Table 27 were prepared similarly as described for Example 182.

Table 27.

Example 193: 9-[l-[[6-Chloro-2-(l-methylpyrazol-4-yl)-3-pyridyl]amino]eth yl]-3-[l-(2- hydroxyacetyl)-4-piperidyl]-4,7-dimethyl-pyrazolo[3,4-c]isoq uinolin-5-one

To a solution of 9-(l-((6-chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3- yl)amino)ethyl)-4,7-dimethyl-3-(piperidin-4-yl)-3,4-dihydro- 5H-pyrazolo[3,4-c]isoquinolin- 5-one (Example 158, step 3) (10 mg, 0.02 mmol) in DMF (0.2 mL) was added 2- hydroxyacetic acid (3 mg, 0.04 mmol), HATU (15 mg, 0.04 mmol) and N,N- diisopropylethylamine (7 mg, 0.06). The reaction mixture was stirred at room temperature for 30 minutes. The resulting mixture was diluted with methanol and purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C ₃₀ H ₃₄ ClN ₈ O ₃ (M+H) ⁺ m/z = 589.2; found 589.2.

Examples 194-197.

Examples 194-197 in Table 28 were prepared similarly as described for Example 193.

Table 28.

Example 198: 9-(l-((6-Chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3-yl)ami no)ethyl)-4- methyl-3-(l-methylpiperidin-4-yl)-5-oxo-4,5-dihydro-3H-pyraz olo[3,4-c]isoquinoline-7- carbonitrile

Step 1: tert-butyl 4-(9-acetyl-7-chloro-4-methyl-5-oxo-pyrazolo[3,4-c]isoquinol in-3- yl)piperidine-l-carboxylate

The titled compound was prepared using similar procedures as described for Example 81 with tert-butyl 4-hydrazineylpiperidine-l -carboxylate replacing tert-butyl 3- hydrazineylazetidine-1 -carboxylate in Step 2, and 3 -bromo-5-chloro-2 -iodobenzoic acid replacing 3-bromo-2-iodo-5-methylbenzoic acid in step 4. The resulting residue was purified by silica gel column chromatography, eluted with ethyl acetate in DCM (0 to 100%) to afford the desired product as a white solid. LCMS calculated for C23H28CIN4O4 (M+H) ⁺ m/z = 459.2, found 459.2.

Step 2: tert-butyl 4-(9-acetyl-7-cyano-4-methyl-5-oxo-pyrazolo[3,4-c]isoquinoli n-3- yl)piperidine-l-carboxylate

To a screw-cap vial equipped with a magnetic stir bar was added tert-butyl 4-(9- acetyl-7-chloro-4-methyl-5-oxo-pyrazolo[3,4-c]isoquinolin-3- yl)piperidine-l-carboxylate (100 mg, 0.22 mmol), /BuXPhos Pd G3 (35 mg, 0.04 mmol), zinc cyanide (13 mg, 0.11 mmol) and zinc formate dihydrate (63 mg, 0.33 mmol). The vial was sealed with a Teflon- lined septum, evacuated, and backfdled with nitrogen three times, then 4 mb DMAc was added. The resulting mixture was heated at 110 °C for 12 h. Upon cooling to room temperature, the mixture was diluted with 20 mb water and 20 mb EtOAc. The aqueous layer was extracted with EtOAc (3 X 10 mL). The combined organic layers were washed with brine (20 mL), dried over anhydrous sodium sulfate. After filtration, the filtrate was concentrated under reduced pressure. The resulting residue was purified by silica gel column chromatography, eluted with ethyl acetate in dichloromethane (0 to 100%) to afford the desired product. LCMS calculated for C24H28N5O4 (M+H) ⁺ m/z = 450.2; found 450.2.

Step 3: tert-butyl 4-(7-cyano-9-(l-hydroxyethyl)-4-methyl-5-oxo-4,5-dihydro-3H- pyrazolo[3, 4-c]isoquinolin-3-yl)piperidine-l-carboxylcite

At 0 °C, to a solution of tert-butyl 4-(9-acetyl-7-cyano-4-methyl-5-oxo-pyrazolo[3,4- c]isoquinolin-3-yl)piperidine-l -carboxylate (80 mg, 0.18 mmol) in the mixture of 2 mL MeOH and 2 mL DCM was added sodium borohydride (10 mg, 0.27 mmol), the resulting solution was stirred at 0 °C for 30 min, then 1 ml 0.5 N HC1 was added, the resulting solution was stirred at the same temperature for 10 min, then poured into the mixture of DCM and water. The aqueous layer was washed with DCM for 3 times. The combined organic layers were washed with brine, dried over anhydrous sodium sulfate. After filtration, the filtrate was concentrated under reduced pressure. The resulting residue was purified by silica gel column chromatography, eluted with ethyl acetate in dichloromethane (0 to 100%) to afford the desired product. LCMS calculated for C24H30N5O4 (M+H) ⁺ m/z = 452.2; found 452.2.

Step 4: tert-butyl 4-[9-(l-bromoethyl)-7-cyano-4-methyl-5-oxo-pyrazolo[3,4-c]is oquinolin-3- yl ] piperidine- 1 -carboxylate

At 0 °C, to a solution of tert-butyl 4-[7-cyano-9-(l-hydroxyethyl)-4-methyl-5-oxo- pyrazolo[3,4-c]isoquinolin-3-yl]piperidine-l-carboxylate (100 mg, 0.22 mmol) in 6 mb THF was added pyridine (0.07 mb, 0.89 mmol), then tribromophosphane (42 uL, 0.44 mmol) dissolved in 1 mb THF was added. The resulting solution was slowly warm to r.t. and stirred for 2 h. The resulting solution was diluted with DCM and water. The aqueous layer was washed with DCM for three times. The combined organic layers were washed with brine (20 mL), dried over anhydrous sodium sulfate. After filtration, the filtrate was concentrated under reduced pressure. The residue was used directly in the next step without further purification.

Step 5: 9-[l-[[6-chloro-2-(l-methylpyrazol-4-yl)-3-pyridyl] amino] ethyl] -4-methyl-5-oxo-3- ( 4-piperidyl)pyrazolo[3, 4-c]isoquinoline- 7 -carbonitrile tert-Butyl 4-[9-(l-bromoethyl)-7-cyano-4-methyl-5-oxo-pyrazolo[3,4-c]is oquinolin- 3-yl]piperidine-l -carboxylate (74 mg, 0.14 mmol) in 2 dram vial was added DMF (0.5 mL), followed by 6-chloro-2-(l-methylpyrazol-4-yl)pyridin-3 -amine (60 mg, 0.29 mmol) The mixture was heated to 60 °C for 3 h. Upon cooling to room temperature, 0.5 mL TFA was added. The resulting solution was stirred for 30 min before diluted with MeOH and purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C28H29CIN9O (M+H) ⁺ m/z = 542.2; found 542.2.

Step 6: 9-[l-[[6-chloro-2-(l-methylpyrazol-4-yl)-3-pyridyl]amino]eth yl]-4-methyl-3-(l- methyl-4-piperidyl)-5-oxo-pyrazolo[3,4-c]isoquinoline-7-carb onitrile

To a solution of 9-[l-[[6-chloro-2-(l-methylpyrazol-4-yl)-3-pyridyl]amino]eth yl]-4- methyl-5-oxo-3-(4-piperidyl)pyrazolo[3,4-c]isoquinoline-7-ca rbonitrile (10 mg, 0.02 mmol) in 1 mL DCM was added formaldehyde (1 uL, 14 umol), followed by sodium triacetoxyborohydride (12 mg, 0.06 mmol). The resulting solution was stirred at r.t. for 1 h, then concentrated under vacuum. The crude product was diluted with MeOH and purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C29H31CIN9O (M+H) ⁺ m/z = 556.2; found 556.2

Example 199: 9-(l-((6-Chloro-2-(l-methyl-LH-l,2,4-triazol-3-yl)pyridin-3- yl)amino)ethyl)-4-ethyl-7-methyl-2-(tetrahydro-2/7-pyran-4-y l)-2,4-dihydro-5/7- pyrazolo [3,4-c] isoquinolin-5-one

Step 1: 9-(l-((6-chloro-2-(l-methyl-lH-l,2,4-triazol-3-yl)pyridin-3- yl)amino)ethyl)-4-ethyl-3-

(4-methoxybenzyl)-7-methyl-3,4-dihydro-5H-pyrazolo[3, 4-c]isoquinolin-5-one

The titled compound was prepared using similar procedures as described for Example

26 with 1-iodoethane replacing iodomethane in step 2 and 6-chloro-2-( I -methyl -I//- 1.2.4- triazol-3-yl)pyridin-3-amine replacing methyl 2 -aminobenzoate in Step 7. The resulting mixture was diluted with water and extracted with EtOAc. The combined organics were washed with sat. NaCl, dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The crude product was used in the next step without purification. LCMS calculated for C31H32CIN8O2 (M+H) ⁺ m/z = 583.2; found 583.2.

Step 2: 9-(l-((6-chloro-2-(l-methyl-lH-l,2,4-triazol-3-yl)pyridin-3- yl)amino)ethyl)-4-ethyl-7- methyl-2, 4-dihydro-5H-pyrazolo[3, 4-c]isoquinolin-5-one

A solution of methyl 9-(l-((6-chloro-2-(l-methyl-lH-l,2,4-triazol-3-yl)pyridin-3- yl)amino)ethyl)-4-ethyl-3-(4-methoxybenzyl)-7-methyl-3,4-dih ydro-5H-pyrazolo[3,4- c]isoquinolin-5-one (60 mg, 0. 1 mmol) in TFA (2 ml) and DCM (2 mL) was stirred at 60 °C for 1 hour. The reaction mixture was concentrated under reduced pressure. The resulting solid was dissolved in sat. NaHCO, and extracted with dichloromethane (2 x 50 mL). The combined organics were washed with sat. NaCl and dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with DCM and ethyl acetate to provide the desired product (26 mg, 56% yield). LCMS calculated for C23H24CIN8O (M+H) ⁺ m/z = 463.2; found 463.2.

Step 3: 9-(l-((6-chloro-2-(l-methyl-lH-l,2,4-triazol-3-yl)pyridin-3- yl)amino)ethyl)-4-ethyl-7- methyl-2-(tetrahydro-2H-pyran-4-yl)-2,4-dihydro-5H-pyrazolo[ 3, 4-c]isoquinolin-5-one

To a solution of 9-( I -((6-chloro-2-( I -methyl- 1 //- 1 ,2.4-triazol -3 -yl )pyridi n-3 - yl)amino)ethyl)-4-ethyl-7-methyl-2,4-dihydro-5H-pyrazolo[3,4 -c]isoquinolin-5-one (8 mg, 0.02 mmol) in DMF (0.2 mL) was added 4-iodotetrahydropyran (18.3 mg, 0.09 mmol) and potassium carbonate (11.9 mg, 0.09 mmol). The reaction mixture was stirred at 80 °C for 8 hours. The mixture was diluted with methanol and purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5μm; mobile phase A: water (0. 1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C28H32CIN8O2 (M+H) ⁺ m/z = 547.2; found: 547.2; Example 200.

Example 200 in Table 29 were prepared similarly as described for Example 199.

Table 29.

Example 201: 9-(l-((6-Chloro-2-(l-methyl-lH-l,2,4-triazol-3-yl)pyridin-3- yl)amino)ethyl)-4-ethyl-7-methyl-2-(l-methylpiperidin-4-yl)- 2,4-dihydro-5/7- pyrazolo [3,4-c] isoquinolin-5-one

Step 1: tert-butyl 4-(9-(l-((6-chloro-2-(l-methyl-lH-l,2,4-triazol-3-yl)pyridin -3- yl)amino)ethyl)-4-ethyl-7-methyl-5-oxo-4,5-dihydro-2H-pyrazo lo[3,4-c]isoquinolin-2- yl)ptperidme-l -carboxylate

The titled compound was prepared using similar procedures as described for Example

26 1-iodoethane replacing iodomethane in step 2, 6-chloro-2-( l -mcthyl-IT/- l .2.4-triazol-3- yl)pyridin-3 -amine replacing methyl 2 -aminobenzoate in Step 7, and l-boc-4- bromopiperidine replacing 1-iodopropane in step 9. The resulting mixture was diluted with water and extracted with EtOAc. The combined organics were washed with sat. NaCl, dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The crude product was used in the next step without purification. LCMS calculated for C33H41CIN9O3 (M+H) ⁺ m/z = 646.3; found 646.3.

Step 2: 9-(l-((6-chloro-2-(l-methyl-lH-l,2,4-triazol-3-yl)pyridin-3- yl)amino)ethyl)-4-ethyl-7- methyl-2-(piperidin-4-yl)-2,4-dihydro-5H-pyrazolo[3, 4-c]isoquinolin-5-one

A solution of tert-butyl 4-(9-( l -((6-chloro-2-( l -mcthyl- IH-l .2.4-triazol-3-yl)pyridin- 3 -yl)amino)ethyl)-4-ethyl-7-methyl-5 -oxo-4,5 -dihydro-2H-pyrazolo [3 ,4-c] isoquinolin-2- yl)piperidine-l -carboxylate (50 mg, 0.08 mmol) in 4N HC1 in dioxane (2 mb) was stirred at room temperature for 30 min. The mixture diluted with MeOH and purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid (22 mg, 51%). LCMS calculated for C28H33CIN9O (M+H) ⁺ m/z = 546.2; found 546.2.

Step 3: 9-(l-((6-chloro-2-(l-methyl-lH-l,2,4-triazol-3-yl)pyridin-3- yl)amino)ethyl)-4-ethyl-7- methyl-2-(l-methylpiperidin-4-yl)-2, 4-dihydro-5H-pyrazolo[3, 4-c]isoquinolin-5-one

To a mixture of 9-( l -((6-chloro-2-( l -mcthyl- IH- l .2.4-triazol-3-yl)pyridin-3- yl)amino)ethyl)-4-ethyl-7-methyl-2-(piperidin-4-yl)-2,4-dihy dro-5H-pyrazolo[3,4- c]isoquinolin-5-one (5 mg, 0.01 mmol) was added DCM (0.5 mb), formaldehyde (water solution, 10 uL) and sodium triacetoxyborohydride (4 mg, 0.02 mmol). The mixture was stirred for 30 min, then diluted with MeOH and purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C29H35CIN9O (M+H) ⁺ m/z = 560.2; found 560.2. Example 202: 2-(l-Acetylpiperidin-4-yl)-9-(l-((6-chloro-2-(l-methyl-LH-l, 2,4-triazol-3- yl)pyridin-3-yl)amino)ethyl)-4-ethyl-7-methyl-2,4-dihydro-5/ 7-pyrazolo[3,4- c]isoquinolin-5-one

To a solution of 9-(l-((6-chloro-2-(l-methyl-lH-l,2,4-triazol-3-yl)pyridin-3- yl)amino)ethyl)-4-ethyl-7-methyl-2-(piperidin-4-yl)-2,4-dihy dro-5H-pyrazolo[3,4- c]isoquinolin-5-one (8 mg, 0.01 mmol) in DCM (0.2 mL) was added acetyl chloride (1.73 mg, 0.015 mmol) and A'.A'-diisopropylcthylaminc (2.84 mg, 0.015 mmol). The mixture was stirred for 30 min, then diluted with MeOH and purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C30H35CIN9O2 (M+H) ⁺ m/z = 588.2; found 588.2.

Example 203: 9-(l-((6-Chloro-2-(l-methyl-lH-l,2,4-triazol-3-yl)pyridin-3- yl)amino)ethyl)-4-ethyl-2-(l-(2-hydroxyethyl)piperidin-4-yl) -7-methyl-2,4-dihydro-5/f- pyrazolo [3,4-c] isoquinolin-5-one

To a mixture of 9-( 1 -((6-chloro-2-( 1 -methyl- 1 //- 1 ,2.4-triazol -3 -yl )pyridi n-3 - yl)amino)ethyl)-4-ethyl-7-methyl-2-(piperidin-4-yl)-2,4-dihy dro-5H-pyrazolo[3,4- c]isoquinolin-5-one (10 mg, 0.02 mmol) was added DCM (0.5 mL), 2-((tert- butyldimethylsilyl)oxy)acetaldehyde (16 mg, 0.09 mmol) and sodium triacetoxyborohydride (11.6 mg, 0.05 mmol). The mixture was stirred for 30 min, solvent was removed under vacuum. 4 M HC1 in dioxane (0.3 mL) and MeOH (0.2 mL) was added and the mixture was stirred for 10 min before diluted with MeOH and purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5μm; mobile phase A: water (0. 1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C30H37CIN9O2 (M+H) ⁺ m/z = 590.3; found 590.3.

Example 204: 3-(4-(9-(l-((6-Chloro-2-(l-methyl-LH-l,2,4-triazol-3-yl)pyri din-3- yl)amino)ethyl)-4-ethyl-7-methyl-5-oxo-4,5-dihydro-2/7-pyraz olo[3,4-c]isoquinolin-2- yl)piperidin-l-yl)propanenitrile

To a solution 9-( 1 -((6-chloro-2-( 1 -methyl - 1 1 ,2.4-triazol-3-yl )pyridin-3- yl)amino)ethyl)-4-ethyl-7-methyl-2-(piperidin-4-yl)-2,4-dihy dro-5H-pyrazolo[3,4- c]isoquinolin-5-one (5 mg, 0.01 mmol) in methanol (0.2 mL) was added prop-2-enenitrile (3 mg, 0.05 mmol). The reaction mixture was stirred at 50 °C for 18 hours before diluted with MeOH and purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C31H36CIN10O (M+H) ⁺ m/z = 599.3; found 599.3.

Example 205: 9-(l-((6-Chloro-2-(l-methyl-LH-l,2,4-triazol-3-yl)pyridin-3- yl)amino)ethyl)-4-ethyl-7-methyl-2-(l-methylazetidin-3-yl)-2 ,4-dihydro-5/f- pyrazolo [3,4-c] isoquinolin-5-one

The titled compound was prepared using similar procedures as described for Example 201 with l-boc-3 -iodoazetidine replacing 1 -boc-4-bromopiperidine in Step 1. The resulting mixture was purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5 μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C27H31CIN9O (M+H) ⁺ m/z = 532.2; found 532.2.

Example 206: 9-(l-((6-Chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3-yl)ami no)ethyl)-

4,7-dimethyl-2-(l-methylpiperidin-4-yl)-2,4-dihydro-5H-py razolo[3,4-c]isoquinolin-5- one

Step 1. tert-butyl 4-(9-(l -((6-chloro-2-(l -methyl- lH-pyrazol-4-yl)pyridin-3-yl)amino)ethyl)- 4, 7-dimethyl-5-oxo-4,5-dihydro-2H-pyrazolo[3,4-c]isoquinolin-2 -yl)piperidine-l-ccirboxylcite

The titled compound was prepared using similar procedures as described for Example 26 with 6-chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3-amine replacing methyl 2- aminobenzoate in Step 7 and tert-butyl 4-iodopiperidine-l -carboxylate replacing 1- iodopropane in Step 9. The mixture was diluted with water and extracted with EtOAc. The combined organics were dried with anhydrous sodium sulfate, fdtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with hexane and ethyl acetate to provide the desired product as a white solid. LCMS calculated for C33H40CIN8O3 (M+H) ⁺ m/z = 631.3; found 631.3.

Step 2. 9-(l-((6-chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3-yl)ami no)ethyl)-4, 7-dimethyl- 2-(piperidin-4-yl)-2,4-dihydro-5H-pyrazolo[3,4-c]isoquinolin -5-one tert-Butyl 4-(9-( 1 -((6-chloro-2-( 1 -methyl-lH-pyrazol-4-yl)pyridin-3 -yl)amino)ethyl)- 4,7-dimethyl-5-oxo-4,5-dihydro-2H-pyrazolo[3,4-c]isoquinolin -2-yl)piperidine-l -carboxylate (10 mg, 0.016 mmol) was dissolved in 1 mb of 30 % TFA in DCM. After stirring for 30 min at r.t., the solvent was removed under vacuum. The residue was used directly in the next step without further purification. LCMS calculated for C28H32CIN8O (M+H) ⁺ m/z = 531.2; found 531.2.

Step 3. 9-(l-((6-Chloro-2-(l -methyl-lH-pyrazol-4-yl)pyridin-3-yl)amino)ethyl)-4, 7-dimethyl- 2-(l-methylpiperidin-4-yl)-2, 4-dihydro-5H-pyrazolo[3, 4-c]isoquinolin-5-one

To a solution of 9-(l-((6-chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3- yl)amino)ethyl)-4,7-dimethyl-2-(piperidin-4-yl)-2,4-dihydro- 5H-pyrazolo[3,4-c]isoquinolin- 5-one (5 mg, 0.01 mmol) in 0.5 mb of ACN and 0.1 mb AcOH was added formaldehyde (6 mg, 0.2 mmol) and sodium triacetoxyhydroborate (7.6 mg, 0.04 mml) at 0 °C. After stirring at r.t. for 30 min, the mixture was diluted with water and extracted with EtOAc. The combined organics were dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The resulting mixture was purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5 μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C29H34CIN8O (M+H) ⁺ m/z = 545.3; found 545.3. Examples 207-208.

Examples 207-208 in Table 30 were prepared similarly as described for Example 206

Table 30. Example 209: 2-(l-Acetylpiperidin-4-yl)-9-(l-((6-chloro-2-(l-methyl-lH-py razol-4- yl)pyridin-3-yl)amino)ethyl)-4,7-dimethyl-2,4-dihydro-5H-pyr azolo[3,4-c]isoquinolin-5- one

The titled compound was prepared using similar procedures as described for Example 202 with 9-(l-((6-chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3-yl)ami no)ethyl)-4,7- dimethyl-2-(piperidin-4-yl)-2,4-dihydro-5H-pyrazolo[3,4-c]is oquinolin-5-one (Example 206, step 2) replacing 9-( I -((6-chloro-2-( I -methyl- 1 1 _2.4-triazol-3-yl)pyridin-3- yl)amino)ethyl)-4-ethyl-7-methyl-2-(piperidin-4-yl)-2,4-dihy dro-5H-pyrazolo[3,4- c]isoquinolin-5-one. The resulting mixture was purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5 μm; mobile phase A: water (0.1% TF A), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C30H34CIN8O2 (M+H) ⁺ m/z = 573.2; found 573.2.

Examples 210-211. Examples 210-211 in Table 31 were prepared similarly as described for Example 209.

Table 31.

Example 212: 9-(l-((6-Chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3-yl)ami no)ethyl)-2- (l-(2,2-difluoroethyl)piperidin-4-yl)-4,7-dimethyl-2,4-dihyd ro-5H-pyrazolo[3,4- c]isoquinolin-5-one

The titled compound was prepared using similar procedures as described for Example 142 with 9-(l-((6-chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3-yl)ami no)ethyl)-4,7- dimethyl-2-(piperidin-4-yl)-2,4-dihydro-5H-pyrazolo[3,4-c]is oquinolin-5-one (Example 206, step 2) replacing 9-(l-((6-chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3-yl)ami no)ethyl)-4,7- dimethyl-3 -(pyrrolidin-3 -yl) -3 ,4-dihydro-5//-py razolo [3 ,4-c]isoquinolin-5 -one . The resulting mixture was purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5 μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C30H34CIF2N8O (M+H) ⁺ m/z = 595.2; found 595.2.

Example 213: 3- [6-Chloro-3- [1- [7-methyl-5-oxo-3- [(35)-tetrahydrofuran-3-yl]-4- (trideuteriomethyl)pyr azolo [3,4-c] isoquinolin-9-yl] ethylamino] -2-pyridyl] -AH- 1 ,2,4- oxadiazol-5-one

Step 1. 9-(l-bromoethyl)-7-methyl-4-(methyl-d3)-3-((S)-tetrahydrofur an-3-yl)-3,4-dihydro- 5H-pyrazolo[3, 4-c]isoquinolin-5-one

The title compound was prepared using similar procedures as described for Example 81 with with [(3R)-tetrahydrofiiran-3-yl]hydrazine replacing tert-butyl 3- hydrazineylazetidine-1 -carboxylate in Step 2, and CD3I replacing iodomethane in step 5.

Step 2. 3-[6-chloro-3-[l -[7 -methyl- 5 -oxo- 3- [( 3S)-tetrahydrofuran-3-yl ]-4- (trideuteriomethyl)pyrazolo [3, 4-c]isoquinolin-9-yl] ethylamino] -2-pyridyl]-4H-l, 2,4- oxadiazol-5-one

9-(l-Bromoethyl)-7-methyl-4-(methyl-d3)-3-((S)-tetrahydro fiiran-3-yl)-3,4-dihydro- 5H-pyrazolo[3,4-c]isoquinolin-5-one (20 mg, 0.06 mmol) in 2 dram vial was added DMF (0.3 mb), and 3-(3-amino-6-chloropyridin-2-yl)-l,2,4-oxadiazol-5(4H)-one (20 mg, 0.09 mmol). The mixture was heated to 80 °C for 2 h. Upon cooling to room temperature, the mixture was diluted with MeOH and purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C25H22D3CIN7O4 (M+H) ⁺ m/z = 525.2; found 525.2.

Examples 214-220.

Examples 214-220 in Table 32 were prepared similarly as described for Example 213.

Table 32.

Example 221: 9-(l-((6-Chloro-2-(l-methyl-lH-l,2,4-triazol-3-yl)pyridin-3- yl)amino)ethyl)-4-cyclopropyl-3-ethyl-7-methyl-3,4-dihydro-5 H-pyrazolo[3,4- c]isoquinolin-5-one

The titled compound was prepared using similar procedures as described for Example 16 with 9-bromo-4-cyclopropyl-3-ethyl-7-methyl-pyrazolo[3,4-c]isoqui nolin-5-one (intermediate 52) replacing 9-bromo-3,4,7-trimethyl-3,4-dihydro-5H-pyrazolo[3,4- c]isoquinolin-5-one in Step 4, and 6-chloro-2-(l -methyl- 1H-1, 2, 4-triazol-3-yl)pyridin-3- amine replacing 2 -aminobenzoic acid in Step 7. The resulting mixture was purified by prep- HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5 μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C26H28CIN8O (M+H) ⁺ m/z = 503.2; found 503.2.

Example 222.

Example 222 in Table 33 were prepared similarly as described for Example 221 Table 33.

Example 223: 9-[l-[[6-Chloro-2-(l-methyl-l,2,4-triazol-3-yl)-3-pyridyl]am ino]ethyl]-3- ethyl-7-methyl-4-tetrahydrofuran-3-yl-pyrazolo[3,4-c]isoquin olin-5-one

The titled compound was prepared using similar procedures as described for Example 16 with 9-bromo-3-ethyl-7-methyl-4-(tetrahydrofuran-3-yl)-3,4-dihydr o-5H-pyrazolo[3,4- c]isoquinolin-5-one (intermediate 53) replacing 9-bromo-3,4,7-trimethyl-3,4-dihydro-5H- pyrazolo[3,4-c]isoquinolin-5-one in Step 4, and 6-chloro-2-(l-methyl-lH-l,2,4-triazol-3- yl)pyridin-3 -amine replacing 2 -aminobenzoic acid in Step 7. The resulting mixture was purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5 μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C27H30CIN8O2 (M+H) ⁺ m/z = 533.2; found 533.2.

Example 224: 9-[l-[[6-Chloro-2-(l-methyl-l,2,4-triazol-3-yl)-3-pyridyl]am ino]ethyl]-3- ethyl-4-(2-hydroxyethyl)-7-methyl-pyrazolo[3,4-c]isoquinolin -5-one The titled compound was prepared using similar procedures as described for Example

16 with 1 -ethyl-lH-pyrazol-5-amme replacing l-methyl-lH-pyrazol-5-amme in Step 1 and tert-Butyl(2-iodoethoxy)dimethylsilane replacing methyl iodide in step 2, 6-chloro-2-(l- methyl-l,2,4-triazol-3-yl)pyridin-3-amine replacing 2-aminobenzoic acid in Step 7. The crude product from Step 7 was dissolved in 4N HC1 and stirred for 30 min before diluted with MeOH and purified by prep-HPEC (column: Sunfire prep C18 column, 30* 150 mm, 5μm; mobile phase A: water (0.1% TEA), mobile phase B: acetonitrile; flow rate: 60 mb/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. ECMS calculated for C25H28CIN8O2 (M+H) ⁺ m/z = 507.2; found 507.1.

Example 225: 4-(6-Chloro-3-(((3-ethyl-4,7-dimethyl-5-oxo-4,5-dihydro-3/f- pyrazolo [3,4- c]isoquinolin-9-yl)methyl)amino)pyridin-2-yl)-2-fluoro-JV-me thylbenzamide

Step 1: 3-ethyl-4, 7-dimethyl-5-oxo-4,5-dihydro-3H-pyrazolo[3,4-c]isoquinoline- 9- carbcildehyde

O xxV N'-'X k '=N

To a stirred solution of 3-ethyl-4,7-dimethyl-9-vinyl-3,4-dihydro-5H-pyrazolo[3,4- c]isoquinolin-5-one (260 mg , 0.97 mmol) (see example 76, step 2), sodium periodate (1.04 g, 4.86 mmol) and 2,6-dimethylpyridine (0.23 mb, 1.95 mmol) in 10 mb THb and 2 mb H2O was added OsCL (19.8 mg , 0.08 mmol) (4% aq. solution) dropwise and the resulting reaction mixture was kept stirred at rt for 3 h. ECMS analysis indicated the reaction was complete. The reaction mixture was diluted with water and extracted with EtOAc. The combined organics were washed with sat. NaCl and dried with anhydrous sodium sulfate, fdtered, and concentrated under reduced pressure to afford the desired product (0.16 g, 58%) which was directly used in the following step without further purification. LCMS calculated for CI ₅ HI ₆ N ₃ O2 (M+H) ⁺ m/z = 270.1; found 270.1.

Step 2: 3-ethyl-9-(hydroxymethyl)-4, 7-dimethyl-3, 4-dihydro-5H-pyrazolo[3, 4-c]isoquinolin- 5-one

To a mixture of 3-ethyl-4,7-dimethyl-5-oxo-4,5-dihydro-3H-pyrazolo[3,4- c]isoquinoline-9-carbaldehyde (150 mg, 0.56 mmol) in THF (6 mL) was added LAH (1.1 mL, 1.1 mmol, 1 M solution in THF) at -78 °C. The resulting mixture was stirred at same temperature for 10 min, and then warmed to -40 °C before quenched with sat. NH4CI. The mixture was diluted with water and extracted with EtOAc. The combined organics were washed with sat. NaCl and dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with DCM and ethyl acetate to provide the desired product as a white solid (125 mg, 83%). LCMS calculated for CI ₅ HI ₈ N ₃ O ₂ (M+H) ⁺ m/z = 272.1; found 272.1.

Step 3: 9-(bromomethyl)-3-ethyl-4, 7-dimethyl-3, 4-dihydro-5H-pyrazolo[3, 4-c]isoquinolin-5- one

To a mixture of 3-ethyl-9-(hydroxymethyl)-4,7-dimethyl-3,4-dihydro-5H- pyrazolo[3,4-c]isoquinolin-5-one (80 mg, 0.29 mmol) in DCM (1 mL) was added PBr ₃ (160 mg, 0.59 mmol). The resulting mixture was stirred at rt for 2 h. The mixture was then quenched with sat. NaHCO ₃ and extracted with DCM. The combined organics were washed with sat. NaCl, dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was used directly in the next step without further purification.

Step 4: 9-(((2-bromo-6-chloropyridin-3-yl)amino)methyl)-3-ethyl-4, 7-dimethyl-3, 4-dihydro- 5H-pyrazolo[3, 4-c]isoquinolin-5-one

To the solution of 9-(bromomethyl)-3-ethyl-4,7-dimethyl-3,4-dihydro-5H- pyrazolo[3,4-c]isoquinolin-5-one (97 mg, 0.29 mmol) in DMF (0.3 mL) was added 2-bromo- 6-chloro-pyridin-3-amine (184 mg, 0.88 mmol). The mixture was heated to 80 °C for 2 h. Upon cooling to room temperature, the mixture was diluted with water and extracted with ethyl acetate (3X). The combined organics were washed with water, sat. NaCl, and dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with DCM and MeOH to provide the desired product as a light yellow solid (75 mg, 54%). LCMS calculated for C2oH2oBrClN50 (M+H) ⁺ m/z = 460.0; found 460.0.

Step 5: 4-(6-chloro-3-(((3-ethyl-4, 7-dimethyl-5-oxo-4,5-dihydro-3H-pyrazolo [3,4- c]isoquinolin-9-yl)methyl)amino)pyridin-2-yl)-2-fluoro-N-met hylbenzamide

A mixture of 9-(((2-bromo-6-chloropyridin-3-yl)amino)methyl)-3-ethyl-4,7- dimethyl-3,4-dihydro-5H-pyrazolo[3,4-c]isoquinolin-5-one (10.0 mg, 0.02 mmol), [3-fluoro- 4-(methylcarbamoyl)phenyl]boronic acid (4.27 mg, 0.02 mmol), cesium carbonate (21.2 mg, 0.07 mmol) and tetrakis(triphenylphosphine)palladium(0) (5.0 mg, 0.004 mmol) in a 5 to 1 mixture of toluene and water (0.2 mL) was heated at 100 °C for 1 h under nitrogen atmosphere. Upon cooling to room temperature, the mixture was concentrated under reduced pressure. The residue was diluted with methanol and purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5μm; mobile phase A: water (0. 1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C28H27CIFN6O2 (M+H) ⁺ m/z = 533.2; found: 533.2;

Example 226: 9-(l-((6-Chloro-2-(3-methyl-LH-pyrazol-4-yl)pyridin-3-yl)oxy )ethyl)-3- ethyl-4,7-dimethyl-3,4-dihydro-5/7-pyrazolo[3,4-c]isoquinoli n-5-one

Step 1 : 9-(l-bromoethyl)-3-ethyl-4, 7-dimethyl-3, 4-dihydro-5H-pyrazolo[3, 4-c]isoquinolin-5- one

The title compound was prepared using similar procedures as described for Example 16 with 1 -ethyl-lH-pyrazol-5-amine replacing l-methyl-lH-pyrazol-5-amine in step 1. The crude product was used in the next step without purification.

Step 2: 9-(l-((2-bromo-6-chloropyridin-3-yl)oxy)ethyl)-3-ethyl-4, 7 -dimethyl- 3, 4-dihydro-5H- pyrazolo[3, 4-c]isoquinolin-5-one

To a solution of 9-(l-bromoethyl)-3-ethyl-4,7-dimethyl-3,4-dihydro-5H- pyrazolo[3,4-c]isoquinolin-5-one (115 mg, 0.33 mmol) in DMF (0.5 mb) was added 2- bromo-6-chloro-pyridin-3-ol (208.2 mg, 1.0 mmol) and potassium carbonate (138.0 mg, 1.0 mmol). The mixture was heated to 60 °C for 16 h. Upon cooling to room temperature, the mixture was diluted with water and extracted with ethyl acetate (3X). The combined organics were washed with water, sat. NaCl, and dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with DCM and MeOH to provide the desired product as a light yellow solid (80 mg, 50%). LCMS calculated for C2iH2iBrClN4C>2 (M+H) ⁺ m/z = 475.1; found 475.1. Step 3: 9-(l-((6-chloro-2-(3-methyl-lH-pyrazol-4-yl)pyridin-3-yl)oxy )ethyl)-3-ethyl-4, 7- dimethyl-3, 4-dihydro-5H-pyrazolo[3, 4-c]isoquinolin-5-one

A mixture of 9-(l-((2-bromo-6-chloropyridin-3-yl)oxy)ethyl)-3-ethyl-4,7-d imethyl- 3,4-dihydro-5H-pyrazolo[3,4-c]isoquinolin-5-one (10 mg, 0.02 mmol), 3 -methyl -4-(4, 4,5,5- tetramethyl-l,3,2-dioxaborolan-2-yl)-lH-pyrazole (4.4 mg, 0.02 mmol), cesium carbonate (20.5 mg, 0.06 mmol) and tetrakis(triphenylphosphine)palladium(0) (4.9 mg, 0.004 mmol) in a 5 to 1 mixture of dioxane and water (0.2 mb) was heated at 100 °C for 1 h under nitrogen atmosphere. Upon cooling to room temperature, the mixture was concentrated under reduced pressure. The residue was diluted with methanol and purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5μm; mobile phase A: water (0. 1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C25H26CIN6O2 (M+H) ⁺ m/z = 477.2; found: 477.2;

Example 227: 3-(6-Chloro-3-((l-(3-ethyl-7-methyl-4-(methyl-d3)-5-oxo-4,5- dihydro-3H- pyrazolo[3,4-c]isoquinolin-9-yl)ethyl)amino)pyridin-2-yl)-l, 2,4-oxadiazol-5(4H)-one

The titled compound was prepared using similar procedures as described for Example 16 with iodomethane -ds replacing iodomethane in Step 2 and 3-(3-amino-6-chloropyridin-2- yl)-l,2,4-oxadiazol-5(4H)-one replacing 2 -aminobenzoic acid in Step 7. The resulting mixture was purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5 μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C23H20D3CIN7O3 (M+H) ⁺ m/z = 483.2; found 483.2

Examples 228-235.

Examples 228-235 in Table 34 were prepared similarly as described for Example 18 Table 34.

Examples 236-240.

Examples 236-240 in Table 35 were prepared similarly as described for Example 60.

Table 35.

Example 241 : 9- [1- [ [2-(l-Acetyl-4-piperidyl)-6-chloro-3-pyridyl] amino] ethyl] -3-ethyl- 4,7-dimethyl-pyrazolo[3,4-c]isoquinolin-5-one

Stepl : tert-butyl 4-(3-amino-6-chloro-2-pyridyl)piperidine-l-carboxylate

Boc

To a solution of 2-bromo-6-chloro-pyridin-3 -amine (300 mg, 1.45 mmol) in 4 mL of THF was added Pd(dppf)C12 (106 mg, 0.14 mmol), followed by ( 1 -tert-butoxycarbonyl -4- piperidyl)-iodo-zinc (0.5 M THF solution, 3.5 mL, 1.74 mmol) dropwise at rt. The resulting solution was then heated to 70 °C for 12 h under nitrogen gas. Upon cooling down to r.t. the crude mixture was diluted with DCM and washed with water. The aqueous layer was extracted with DCM for 3 times. The combined organic layer was washed with brine and dried over sodium sulfate. Column purified: EtOAc/DCM= 0% to 100% to get desired product. LCMS calculated for C11H15CIN3O2 [M-56+H] ⁺ 256.1, found 256.1

Step 2: 9-[l-[[6-chloro-2-(4-piperidyl)-3-pyridyl]amino]ethyl]-3-eth yl-4, 7-dimethyl- pyrazolo[3, 4-c]isoquinolin-5-one

The titled compound was prepared using similar procedures as described for Example 16 with I -cthyl-IH-pyrazol-5-aminc replacing l -mcthyl-IH-pyrazol-5-aminc in Step 1 and tert-butyl 4-(3-amino-6-chloro-2-pyridyl)piperidine-l -carboxylate replacing 2-aminobenzoic acid in Step 7. The crude product was dissolved the mixture of DCM/TFA(1: 1) and stirred for 30 min. After concentration under the vacuum, the crude product was diluted with MeOH and purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C26H32CIN6O (M+H) ⁺ m/z = 479.2 found 479.1.

Step 3: 9-[l -[[2-(l -acetyl-4-piperidyl)-6-chloro-3-pyridyl ] amino] ethyl ]-3-ethyl-4, 7-dimethyl- pyrazolo[3, 4-c]isoquinolin-5-one

To a solution of 9-[l-[[6-chloro-2-(4-piperidyl)-3-pyridyl]amino]ethyl]-3-eth yl-4,7- dimethyl-pyrazolo[3,4-c]isoquinolin-5-one (10 mg, 0.02 mmol) in ImL of DCM was added A-cthyl-A-isopropyl-propan-2 -amine (18 uL, 0.10 mmol) . Then acetyl chloride (4 uL, 0.06 mmol) dissolved in 0.5 mb DCM was added dropwise at 0 °C. The resulting solution was stirred at 0 °C for 15 min and then concentrated under vacuum. The crude product was diluted with MeOH and purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C28H34CIN6O2 (M+H) ⁺ m/z = 521.2; found page 521.2

Example 242: 9-(l-((6-Chloro-2-(l-methyl-lH-l,2,4-triazol-3-yl)pyridin-3- yl)amino)ethyl)-3-(2-hydroxyethyl)-4,7-dimethyl-3,4-dihydro- 5H-pyrazolo[3,4- c]isoquinolin-5-one

The titled compound was prepared using similar procedures as described for Example 16 with l-(2-((triisopropylsilyl)oxy)ethyl)-lH-pyrazol-5-amine (intermediate 51) replacing 1- methyl-lH-pyrazol-5-amine in Step 1, and 6-chloro-2-(l-methyl-lH-l,2,4-triazol-3- yl)pyridin-3 -amine replacing 2 -aminobenzoic acid in Step 7. The resulting mixture was diluted with 1 mL THF and tetrabutylammonium fluoride (THF solution, 86.44 uL, 0.09 mmol) was added to the reaction mixture at 0 °C. The reaction mixture was stirred at 0 °C for 30 min. LCMS analysis indicated the reaction was complete. The reaction was quenched with water and extracted with EtOAc. The combined organics were concentrated under reduced pressure. The residue was purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C24H26CIN8O2 (M+H) ⁺ m/z = 493.2; found 493.2.

Examples 243-252.

Examples 243-252 in Table 36 were prepared similarly as described for Example 242.

Table 36.

Example 253. 5- [6-Chloro-3- [1- [3-(2-hydroxyethyl)-4,7-dimethyl-5-oxo-pyrazolo [3,4- c]isoquinolin-9-yl]ethylamino]-2-pyridyl]-N-methyl-pyridine- 2-carboxamide Step 1: 9-[l-[(2-bromo-6-chloro-3-pyridyl)amino] ethyl] -3-(2-hydroxyethyl)-4, 7-dimethyl- pyrazolo[3, 4-c]isoquinolin-5-one

The titled compound was prepared using similar procedures as described for Example 16 with l-(2-((triisopropylsilyl)oxy)ethyl)-lH-pyrazol-5-amine (Intermediate 51) replacing 1- methyl -IH-pyrazol -5 -amine in Step 1, and 2-bromo-6-chloro-pyridin-3-amine replacing 2- aminobenzoic acid in Step 7. The resulting mixture was diluted with 1 mL THF and tetrabutylammonium fluoride (THF solution, 86.44 uL, 0.09 mmol) was added to the reaction mixture at 0 °C. The reaction was stirred at 0 °C for 30 min then quenched with water and extracted with EtOAc. The combined organics were concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with DCM and MeOH to provide the desired product. LCMS calculated for C2iH22BrQN5C>2 (M+H) ⁺ m/z = 490.1found 490.0 Step 2: 5-[6-chloro-3-[l-[3-(2-hydroxyethyl)-4, 7-dimethyl-5-oxo-pyrazolo[3, 4-c]isoquinolin- 9-yl] ethylamino] -2-pyridyl]-N-methyl-pyridine-2-carhoxamide

A mixture of 9-[l-[(2-bromo-6-chloro-3-pyridyl)amino]ethyl]-3-(2-hydroxye thyl)- 4,7-dimethyl-pyrazolo[3,4-c]isoquinolin-5-one (12.5 mg, 0.03 mmol), tetrakis(triphenylphosphine)palladium(0) (5.9 mg, 0.01 mmol), K3PO4 (16.2 mg, 0.08 mmol) and N-methyl -5-(4, 4, 5, 5 -tetramethyl- 1, 3, 2-dioxaborolan-2-yl)pyridine-2 -carboxamide (8.01 mg, 0.03 mmol) in 1 mb dioxane/H2O(5: 1) was bubbled with N2 for 1 min. The reaction mixture was stirred at 80 °C for 12 h under nitrogen atmosphere. LCMS analysis indicated the reaction was complete. The reaction was quenched with water and extracted with EA. The combined organics were concentrated under reduced pressure. The residue was purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a light yellow solid. LCMS calculated for C28H29CIN7O3 (M+H) ⁺ m/z = 546.2; found 546.2.

Examples 254-256.

Examples 254-256 in Table 37 were prepared similarly as described for Example 253.

Table 37.

Example 257. 9-[l-[[6-Chloro-2-(l-methyl-4-piperidyl)-3-pyridyl]amino]eth yl]-3-(2- hydroxyethyl)-4,7-dimethyl-pyrazolo[3,4-c]isoquinolin-5-one Step 1: 9-[l-[[6-chloro-2-(4-piperidyl)-3-pyridyl]amino]ethyl]-3-(2- hydroxyethyl)-4, 7- dimethyl-pyrazolo[3,4-c]isoquinolin-5-one

The titled compound was prepared using similar procedures as described for Example 16 with l-(2-((triisopropylsilyl)oxy)ethyl)-lH-pyrazol-5-amine (Intermediate 51) replacing 1- methyl -IH-pyrazol -5 -amine in Step 1, and tert-butyl 4-(3-amino-6-chloro-2- pyridyl)piperidine-l -carboxylate (Example 241, step 1) replacing 2-aminobenzoic acid in Step 7. The reaction was quenched with 1 mb water and extracted with 2 x 1 mb EA. The organics were concentrated to dryness. The residue was dissolved in 1 mb 4N HC1 in dioxane and the resulting mixture was kept stirred at rt for 2 h. ECMS analysis indicated the reaction was complete. The crude was diluted with MeOH and purified by prep-HPEC (column: Sunfire prep C18 column, 30* 150 mm, 5 μm; mobile phase A: water (0.1% TEA), mobile phase B: acetonitrile; flow rate: 60 mb/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product (29.00 mg, 61.0 %). ECMS calculated for C26H ₃ 2C1N ₆ O ₂ (M+H) ⁺ m/z = 495.2; found 495.2.

Step 2: 9-[l-[[6-chloro-2-(l-methyl-4-piperidyl)-3-pyridyl]amino]eth yl]-3-(2-hydroxyethyl)- 4, 7-dimethyl-pyrazolo[3, 4 -c] isoquinolin- 5 -one

To a stirred solution of 9-[l-[[6-chloro-2-(4-piperidyl)-3-pyridyl]amino]ethyl]-3-(2- hydroxyethyl)-4,7-dimethyl-pyrazolo[3,4-c]isoquinolin-5-one (7.00 mg, 0.01 mmol) in 0.5 mb DCM was added formaldehyde (5.74 uh, 0.07 mmol) and the resulting mixture was kept stirred at rt for 15 min. Sodium triacetoxyborohydride (8.99 mg, 0.04 mmol) was then added to the reaction mixture and the resulting mixture was kept stirred at rt for Ih. ECMS analysis indicated the reaction was complete. The crude was purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5 μm; mobile phase A: water (0. 1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product. LCMS calculated for C27H34CIN6O2 (M+H) ⁺ m/z = 509.2. found: 509.2.

Example 258. 9- [1- [ [2-(l-Acetyl-4-piperidyl)-6-chloro-3-pyridyl] amino] ethyl] -3-(2- hydroxyethyl)-4,7-dimethyl-pyrazolo[3,4-c]isoquinolin-5-one

To a stirred solution of 9-[l-[[6-chloro-2-(4-piperidyl)-3-pyridyl]amino]ethyl]-3-(2- hydroxyethyl)-4,7-dimethyl-pyrazolo[3,4-c]isoquinolin-5-one (Example 257, step 1) (7.00 mg, 0.01 mmol) and acetyl chloride (1.47 uL, 0.02 mmol) in 1 mL DCM was added A'-cthyl- A-isopropyl-propan-2 -amine (7.6 uL, 0.04 mmol) and the resulting mixture was kept stirred at rt for Ih. LCMS analysis indicated the reaction was complete. The crude was diluted with MeOH and purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5 μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product. LCMS calculated for C^lFuCIM.Ch (M+H) ⁺ m/z = 537.2. found: 537.2.

Example 259. 9-[l-[[6-Chloro-2-(l-methyl-l,2,4-triazol-3-yl)-3-pyridyl]am ino]ethyl]-4- (2,2-difluoroethyl)-3-(2-hydroxyethyl)-7-methyl-pyrazolo[3,4 -c]isoquinolin-5-one

The titled compound was prepared using similar procedures as described for Example 16 with l-(2-((triisopropylsilyl)oxy)ethyl)-lH-pyrazol-5-amine (intermediate 51) replacing 1- methyl-lH-pyrazol-5-amine in Step 1, l,l-difluoro-2 -iodoethane replacing iodomethane in Step 2, and 6-chloro-2-(l-methyl-lH-l,2,4-triazol-3-yl)pyridin-3-amine replacing 2- aminobenzoic acid in Step 7. The product was deprotected with TBAF. The crude product was purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5 μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C25H26CIF2N8O2 (M+H) ⁺ m/z = 543.2; found 543.2. Example 260. 5- [6-Chloro-3- [1- [4-(2,2-difluoroethyl)-3-(2-hydroxyethyl)-7-methyl-5- oxo-pyrazolo [3,4-c] isoquinolin-9-yl] ethylamino] -2-pyridyl] -N-methyl-pyridine-2- carboxamide

Step 1 : 9-[l-[(2-bromo-6-chloro-3-pyridyl)amino] ethyl] -4-(2, 2-difluoroethyl)-3-(2- hydroxyethyl)- 7-methyl-pyrazolo[3, 4-c]isoquinolin-5-one

The titled compound was prepared using similar procedures as described for Example 16 with l-(2-((triisopropylsilyl)oxy)ethyl)-lH-pyrazol-5-amine (intermediate 51) replacing 1- methyl-lH-pyrazol-5-amine in Step 1, l,l-difluoro-2 -iodoethane replacing iodomethane in Step 2, and 2-bromo-6-chloropyridin-3-amine replacing 2-aminobenzoic acid in Step 7. The product was deprotected with TBAF. The crude product was purified by silica gel column chromatography, eluted with DCM and MeOH to provide the desired product (49.0 mg, 79.0 %) as a white solid. LCMS calculated for C22H23BrClF2N5C>2 (M+H) ⁺ m/z = 540. 1; found

540.1.

Step 2: 5-[6-chloro-3-[l-[4-(2,2-difhioroethyl)-3-(2-hydroxyethyl)-7 -methyl-5-oxo- pyrazolo[3, 4-c]isoquinolin-9-yl] ethylamino] -2-pyridyl]-N-methyl-pyridine-2-carboxamide

A mixture of 9-[l-[(2-bromo-6-chloro-3-pyridyl)amino]ethyl]-4-(2,2-difluo roethyl)- 3-(2-hydroxyethyl)-7-methyl-pyrazolo[3,4-c]isoquinolin-5-one (15.00 mg, 0.03 mmol), tetrakis(triphenylphosphine)palladium(0) (6.41 mg, 0.01 mmol), tripotassium phosphate (17.66 mg, 0.08 mmol) and A-methyl-5-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2- yl)pyridine-2 -carboxamide (10.91 mg, 0.04 mmol) in 1 mb dioxane/H2O(5: 1) was bubbled with N2 for 1 min. The reaction mixture was stirred at 80 °C for 12 h under nitrogen atmosphere. LCMS analysis indicated the reaction was complete. The reaction was quenched with water and extracted with EA. The combined organics were concentrated under reduced pressure. The residue was purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a light yellow solid. LCMS calculated for C29H29CIF2N7O3 (M+H) ⁺ m/z = 596.2; found 596.2.

Example 261. 9-[l-[[6-Chloro-2-(l-methyl-l,2,4-triazol-3-yl)-3-pyridyl]am ino]ethyl]-4- ethyl- 3-(2-hydroxyethyl)-7-methyl-pyrazolo[3,4-c]isoquinolin-5-one

The titled compound was prepared using similar procedures as described for Example 16 with l-(2-((triisopropylsilyl)oxy)ethyl)-lH-pyrazol-5-amine (intermediate 51) replacing 1- methyl-lH-pyrazol-5-amine in Step 1, iodoethane replacing iodomethane in Step 2, and 6- chloro-2-(l-methyl-lH-l,2,4-triazol-3-yl)pyridin-3-amine replacing 2-aminobenzoic acid in Step 7. The product was deprotected with TBAF. The crude product was purified by prep- HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5 μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C25H28CIN8O2 (M+H) ⁺ m/z = 507.2; found 507.2.

Example 262. 9-[l-[(6-Chloro-2-morpholino-3-pyridyl)amino]ethyl]-4-ethyl- 3-(2- hydroxyethyl)-7-methyl-pyrazolo[3,4-c]isoquinolin-5-one

The titled compound was prepared using similar procedures as described for Example 16 with l-(2-((triisopropylsilyl)oxy)ethyl)-lH-pyrazol-5-amine (intermediate 51) replacing 1- methyl-lH-pyrazol-5-amine in Step 1, iodoethane replacing iodomethane in Step 2, and 6- chloro-2-morpholinopyridin-3-amine replacing 2-aminobenzoic acid in Step 7. The product was deprotected with TBAF. The crude product was purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5 μm; mobile phase A: water (0. 1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C26H32CIN6O3 (M+H) ⁺ m/z = 511.2; found 511.2.

Example 263. 9-[l-[[6-Chloro-2-(l-methylpyrazol-4-yl)-3-pyridyl]amino]eth yl]-4,7- dimethyl-3-(2-morpholinoethyl)pyrazolo[3,4-c]isoquinolin-5-o ne

To a stirred solution of 9-[l-[[6-chloro-2-(l-methylpyrazol-4-yl)-3- pyridyl] amino] ethyl] -3 -(2-hydroxyethyl)-4,7-dimethyl-pyrazolo [3 ,4-c]isoquinolin-5 -one (Example 244) (35 mg, 0.07 mmol), methanesulfonyl chloride (8 uL, 12.22 mg, 0.11 mmol) in 2 mb DCM was added N,N-diethylethanamine (29.75 uL, 0.21 mmol) and the resulting mixture was kept stirred at rt for 2 h. The reaction mixture was then concentrated to dryness under reduced pressure. A solution of the residue in 0.2 mb DMF was added morpholine (11 mg, 0.13 mmol) and the resulting mixture was kept stirred at rt for 4h. LCMS analysis indicated the reaction was complete. The reaction mixture was diluted with MeOH and purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C29H34CIN8O2 (M+H) ⁺ m/z = 561.2; found 561.2.

Examples 264-268.

Examples 264-268 in Table 38 were prepared similarly as described for Example 263.

Table 38.

Example 269: 9-[l-[[6-Chloro-2-(l-methyl-l,2,4-triazol-3-yl)-3-pyridyl]am ino]ethyl]-4- ethyl-7-methyl-3-(2-pyridyl)pyrazolo[3,4-c]isoquinolin-5-one Step 1: 9-(l-bromoethyl)-4-ethyl-7-methyl-3-(2-pyridyl)pyrazolo[3,4- c]isoquinolin-5-one

The title compound was prepared using similar procedures as described for Example 81 with ethyl 5-amino-l-(2-pyridyl)pyrazole-4-carboxylate replacing ethyl 5-amino-l-(l- (tert-butoxycarbonyl)azetidin-3-yl)-lH-pyrazole-4-carboxylat e in Step 3, and iodoethane replacing Mel in step 5. The residue was used directly in the next step without further purification.

Step 2: 9-[l-[[6-chloro-2-(l-methyl-l,2, 4-triazol-3-yl)-3-pyridyl]amino]ethyl]-4-ethyl-7- methyl-3-(2-pyridyl)pyrazolo[3, 4-c]isoquinolin-5-one

To a stirred mixture of 9-(l-bromoethyl)-4-ethyl-7-methyl-3-(2-pyridyl)pyrazolo[3,4- c]isoquinolin-5-one (10 mg, 0.02 mmol) in 0.1 mL DMF was added 6-chloro-2-(l -methyl - l,2,4-triazol-3-yl)pyridin-3-amine (10 mg, 0.05 mmol). The mixture was stirred at 65°C for 2 h. The residue was diluted with MeOH and then purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5μm; mobile phase A: water (0. 1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product (3.40 mg, 25.9 %) as a white solid. LCMS calculated for C28H27CIN9O (M+H) ⁺ m/z = 540.2; found 540.1.

Examples 270-273.

Examples 270-273 in Table 39 were prepared similarly as described for Example 269.

Table 39.

Example 274: 9-[l-[[6-Chloro-2-(l-methyl-l,2,4-triazol-3-yl)-3-pyridyl]am ino]ethyl]-4- ethyl-7-methyl-3-(3-pyridyl)pyrazolo[3,4-c]isoquinolin-5-one The titled compound was prepared using similar procedures as described for Example

81 with 3 -pyridylhydrazine replacing tert-butyl 3 -hydrazineylazetidine-1 -carboxylate in Step 2, iodoethane replacing iodomethane in step 5, and 6-chloro-2-(l -methyl- 1H-1, 2, 4-triazol-3- yl)pyridin-3 -amine replacing 6-chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3-amine in step 10. The crude product A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C28H27CIN9O (M+H) ⁺ m/z = 540.2; found 540.1.

Example 275: 9-[l-[[6-Chloro-2-(l-methylpyrazol-4-yl)-3-pyridyl]amino]eth yl]-4,7- dimethyl-3-(2-pyridylmethyl)pyrazolo[3,4-c]isoquinolin-5-one

The titled compound was prepared using similar procedures as described for Example 81 with 2-(hydrazineylmethyl)pyridine replacing tert-butyl 3-hydrazineylazetidine-l- carboxylate in Step 2. The crude product A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C29H28CIN8O (M+H) ⁺ m/z = 539.2; found 539.1.

Example 276. 3-(6-Chloro-3-((l-(4,7-dimethyl-5-oxo-3-(pyridin-2-ylmethyl) -4,5-dihydro-

3H-pyrazolo[3,4-c]isoquinolin-9-yl)ethyl)amino)pyridin-2- yl)-l,2,4-oxadiazol-5(4H)-one

The titled compound was prepared using similar procedures as described for Example 81 with 2-(hydrazineylmethyl)pyridine replacing tert-butyl 3-hydrazineylazetidine-l- carboxylate in Step 2, and 3-(3-amino-6-chloropyridin-2-yl)-l,2,4-oxadiazol-5(4H)-one replacing 6-chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3-amine in step 10 The crude product A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C27H24CIN8O3 (M+H) ⁺ m/z = 543.2; found 543.1. Example 277: 9-[l-[[6-Chloro-2-(l-methyl-l,2,4-triazol-3-yl)-3-pyridyl]am ino]ethyl]-4,7- dimethyl-3-(l-methyl-3-piperidyl)pyrazolo[3,4-c]isoquinolin- 5-one

Step 1: benzyl 3-[9-(l-bromoethyl)-4, 7-dimethyl-5-oxo-pyrazolo[3,4-c]isoquinolin-3- yl ] piperidine- 1 -carboxylate

The titled compound was prepared using similar procedures as described for Example 81 with benzyl 3 -hydrazinopiperidine-1 -carboxylate replacing tert-butyl 3- hydrazineylazetidine-1 -carboxylate in Step 2. The crude product was used directly in next step without further purification.

Step 2: 9-[l-[[6-chloro-2-(l-methyl-l,2, 4-triazol-3-yl)-3-pyridyl] amino] ethyl] -4, 7-dimethyl- 3-(3-piperidyl)pyrazolo[3, 4-c]isoquinolin-5-one

To a stirred solution of benzyl 3-[9-(l-bromoethyl)-4,7-dimethyl-5-oxo-pyrazolo[3,4- c]isoquinolin-3-yl]piperidine-l -carboxylate (80.00 mg, 0.15 mmol) in 0.3 mb DMF was added 6-chloro-2-(l-methyl-l,2,4-triazol-3-yl)pyridin-3-amine (46.81 mg, 0.22 mmol) and the resulting mixture was kept stirred at 65 °C for 4 h. The reaction was quenched with water and extracted with EA. The crude was concentrated and 1 mb HBr in acetic acid (33 wt. %) was added at 0 °C. The resulting mixture kept stirred at 0 °C for 15 min. The reaction mixture was diluted with MeOH, and purified by prep-HPLC (column: Sunfire prep Cl 8 column, 30* 150 mm, 5 gm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product (42.00 mg, 53.0 %) as a white solid. LCMS calculated for C27H31CIN9O (M+H) ⁺ m/z = 532.2; found 532.2.

Step 3: 9-[l-[[6-chloro-2-(l-methyl-l,2, 4-triazol-3-yl)-3-pyridyl]amino]ethyl]-4, 7-dimethyl- 3-(l-methyl-3-piperidyl)pyrazolo[3, 4-c]isoquinolin-5-one

To a stirred solution of 9-[l-[[6-chloro-2-(l-methyl-l,2,4-triazol-3-yl)-3- pyridyl] amino] ethyl] -4,7-dimethyl-3 -(3 -piperidyl)pyrazolo [3 ,4-c]isoquinolin-5 -one (8.00 mg, 0.02 mmol) in 0.5 mb DCM was added formaldehyde (6.10 uL, 2.26 mg, 0.08 mmol) and the resulting mixture was kept stirred at rt for 15 min. sodium triacetoxyborohydride (9.56 mg, 0.05 mmol) was then added to the reaction mixture and the resulting mixture was kept stirred at rt for Ih. LCMS analysis indicated the reaction was complete. The crude was purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5 μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product. LCMS calculated for C28H33CIN9O (M+H) ⁺ m/z = 546.2. found: 546.2.

Examples 278-281.

Examples 278-281 in Table 40 were prepared similarly as described for Example 277.

Table 40.

Example 282. 5- [6-Chloro-3- [1- [4,7-dimethyl-3-(l-methyl-4-piperidyl)-5-oxo- pyrazolo[3,4-c]isoquinolin-9-yl]ethoxy]-2-pyridyl]-N-methyl- pyridine-2-carboxamide Step 1: benzyl 4-[9-[l-[(2-bromo-6-chloro-3-pyridyl)oxy]ethyl]-4, 7-dimethyl-5-oxo- pyrazolo[3, 4-c]isoquinolin-3-yl]piperidine-l-ccirboxylate

To a stirred solution of 2-bromo-6-chloro-pyridin-3-ol (38.78 mg, 0.19 mmol) in 0.2 mL DMF was added sodium hydride (6.70 mg , 0.17 mmol) and the resulting mixture was kept stirred at room temperature (rt) for 1 h. Benzyl 4-[9-(l-bromoethyl)-4,7-dimethyl-5-oxo- pyrazolo[3,4-c]isoquinolin-3-yl]piperidine-l-carboxylate (Example 158, step 1) (50.0 mg, 0.09 mmol) was then added to the reaction mixture and the resulting mixture was kept stirred at 65 °C for 12 h. LCMS analysis indicated the reaction was complete. The reaction was quenched with water and extracted with 2 x 2 mL ethyl acetate (EA). The combined organics were concentrated to dryness and the residue was purified by silica gel column chromatography, eluted with dichloromethane (DCM) and MeOH to provide the desired product (42.00 mg, 67.9 %) as a white solid. LCMS calculated for C32H32BrQN5C>4 (M+H) ⁺ m/z = 664.1/666.1; found 664.1/666.1.

Step 2: 5-[6-chloro-3-[l-[4, 7-dimethyl-5-oxo-3-(4-piperidyl)pyrazolo[3, 4-c]isoquinolin-9- ylj ethoxy] -2-pyridyl]-N-methyl-pyridine-2-carboxamide

A mixture of benzyl 4-[9-[l-[(2-bromo-6-chloro-3-pyridyl)oxy]ethyl]-4,7-dimethyl - 5-oxo-pyrazolo[3,4-c]isoquinolin-3-yl]piperidine-l-carboxyla te (40.0 mg, 0.06 mmol), tetrakis(triphenylphosphine)palladium(0) (13.9 mg, 0.01 mmol), tripotassium phosphate (38.3 mg, 0.18 mmol) and A-methyl-5-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2-yl)pyri dine-2- carboxamide (18.92 mg, 0.07 mmol) in 1.5 mL dioxane/H2O(5: 1) was bubbled with N2 for 1 min. The reaction mixture was stirred at 80 °C for 12 h under nitrogen atmosphere. LCMS analysis indicated the reaction was complete. The reaction was quenched with water and extracted with EA. The crude was concentrated and 1 mL HBr in acetic acid (33 wt. %) was added at 0 °C. The resulting mixture kept stirred at 0 °C for 15 min. The reaction mixture was diluted with MeOH and purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5 μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product (22 mg, 62.4 %) as a white solid. LCMS calculated for C31H33CIN7O3 (M+H) ⁺ m/z = 586.2; found 586.2.

Step 3: 5-[6-chloro-3-[l-[4, 7-dimethyl-3-(l-methyl-4-piperidyl)-5-oxo-pyrazolo[3,4- c]isoquinolin-9-yl] ethoxy] -2-pyridyl] -N-methyl-pyridine-2-carboxcimide To a stirred solution of 5-[6-chloro-3-[l-[4,7-dimethyl-5-oxo-3-(4- piperidyl)pyrazolo[3,4-c]isoquinolin-9-yl]ethoxy]-2-pyridyl] -/V-methyl-pyridine-2- carboxamide (7.00 mg , 0.01 mmol) in 0.5 mL DCM was added formaldehyde (4.85 uL, 1.79 mg , 0.06 mmol) and the resulting mixture was kept stirred at rt for 15 min. sodium triacetoxyborohydride (7.59 mg , 0.04 mmol) was then added to the reaction mixture and the resulting mixture was kept stirred at rt for Ih. LCMS analysis indicated the reaction was complete. The crude was purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5 μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product (4.60 mg, 64.2 %). LCMS calculated for C32H35CIN7O3 (M+H) ⁺ m/z = 600.2. found: 600.2.

Example 283: 5-[3-[l-[3-(l-Acetyl-4-piperidyl)-4,7-dimethyl-5-oxo-pyrazol o[3,4- c]isoquinolin-9-yl]ethoxy]-6-chloro-2-pyridyl]-N-methyl-pyri dine-2-carboxamide

To a stirred solution of 5-[6-chloro-3-[l-[4,7-dimethyl-5-oxo-3-(4- piperidyl)pyrazolo[3,4-c]isoquinolin-9-yl]ethoxy]-2-pyridyl] -A-methyl-pyridine-2- carboxamide (7.00 mg , 0.01 mmol) in 0.5 mL DCM was added A-cthyl-A-isopropyl-propan- 2 -amine (6.62 uL, 4.63 mg , 0.04 mmol) and acetyl chloride (1.28 uL, 1.41 mg , 0.02 mmol) and the resulting mixture was kept stirred at rt for lOmin. LCMS analysis indicated the reaction was complete. The crude was diluted with MeOH and purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5 μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product. LCMS calculated for C33H35CIN7O4 (M+H) ⁺ m/z = 628.2; found 628.2. Example 284: 10-(l-((6-Chloro-2-(l-methyl-lH-l,2,4-triazol-3-yl)pyridin-3 - yl)amino)ethyl)-3-(hydroxymethyl)-8-methyl-4,5-dihydro-3H,6H -2,2a,5a- triazaaceanthrylen-6-one

Step 1: 9-bromo-4-(3-((tert-butyldimethylsilyl)oxy)propyl)-3-(4-meth oxybenzyl)-7-methyl-3,4- dihydro-5H-pyrazolo[3, 4-c]isoquinolin-5-one

The titled compound was prepared using similar procedures as described from Step 1 to Step 3 of Example 26 with (3-bromopropoxy)(tert-butyl)dimethylsilane replacing iodomethane in Step 2. The resulting mixture was diluted with water and extracted with EtOAc. The combined organics were washed with sat. NaCl, dried with anhydrous sodium sulfate, fdtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with DCM and ethyl acetate to provide the desired product as a white solid. LCMS calculated for C28H3?BrN3O3Si (M+H) ⁺ m/z = 570.2; found 570.2.

Step 2: 9-bromo-4-(3-hydroxypropyl)-3-(4-methoxybenzyl)-7-methyl-3, 4-dihydro-5H- pyrazolo[3, 4-c]isoquinolin-5-one

9-Bromo-4-(3-((tert-butyldimethylsilyl)oxy)propyl)-3-(4-m ethoxybenzyl)-7 -methyl- 3,4-dihydro-5H-pyrazolo[3,4-c]isoquinolin-5-one (3.7 g, 6.5 mmol) was dissolved in 60 mb of 4N HC1 in dioxane. After stirring for 30 min at r.t., the mixture was diluted with water and extracted with EtOAc. The combined organics were dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with hexanes and ethyl acetate to provide the desired product as a white solid (2.8 g, 94% yield). LCMS calculated for C22H23BrN3C>3 (M+H) ⁺ m/z = 456.1; found 456.1.

Step 3: 3-(9-bromo-3-(4-methoxybenzyl)-7-methyl-5-oxo-3, 5-dihydro-4H-pyrazolo[3, 4- c]isoquinolin-4-yl)propanal

To a solution of 9-bromo-4-(3-hydroxypropyl)-3-(4-methoxybenzyl)-7-methyl-3,4 - dihydro-5H-pyrazolo[3,4-c]isoquinolin-5-one (2.8 g, 6.14 mmol) in 100 mb of DCM was added Dess-Martin periodinane (3.9 g, 9.2 mmol) at 0 °C. After stirring at r.t. for overnight, the mixture was diluted with water and extracted with EtOAc. The combined organics were washed with NaHSO; and brine, then dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with hexanes and ethyl acetate to provide the desired product as a white solid (1.5 g, 54% yield). LCMS calculated for C22H2iBrN3O3 (M+H) ⁺ m/z = 454.1; found 454.1.

Step 4: 9-bromo-4-(3-hydroxypent-4-en-l-yl)-3-(4-methoxybenzyl)-7-me thyl-3, 4-dihydro-5H- pyrazolo[3, 4-c]isoquinolin-5-one

To a solution of 3-(9-bromo-3-(4-methoxybenzyl)-7-methyl-5-oxo-3,5-dihydro-4H - pyrazolo[3,4-c]isoquinolin-4-yl)propanal (1.0 g, 2.2 mmol) in 20 mb of THF was slowly added vinylmagnesium bromide solution (3.3 mb, IM in THF, 3.3 mmol) at -15 °C. After stirring at -15 °C for 1.5h, the mixture was quenched with sat.NHK’l and extracted with EtOAc. The combined organics were dried with anhydrous sodium sulfate, fdtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with hexanes and ethyl acetate to provide the desired product as a white solid (1.0 g, 94% yield). LCMS calculated for C24H25BrN3C>3 (M+H) ⁺ m/z = 482.1; found 482.1.

Step 5: 5-(9-bromo-7-methyl-5-oxo-3,5-dihydro-4H-pyrazolo[3, 4-c]isoquinolin-4-yl)pent-l- en-3-yl ethyl carbonate

To a solution of 9-bromo-4-(3-hydroxypent-4-en-l-yl)-3-(4-methoxybenzyl)-7- methyl-3,4-dihydro-5H-pyrazolo[3,4-c]isoquinolin-5-one (1.0 g, 2.07 mmol) in 20 mb of DCM was added pyridine (0.5 mb, 6.22 mmol) and ethyl carbonochloridate (0.4 mb, 4.15 mmol) at 0 °C. After stirring at r.t. for 2 h, the mixture was diluted with water and extracted with DCM. The combined organics were dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was dissolved in 10 mb of 50 % TEA in DCM at 0 °C. After stirring at 60 °C for 30 min, the mixture was quenched with sat. NaHCCh and extracted with DCM. The combined organics were dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with dichloromethane and ethyl acetate to provide the desired product as a white solid (645 mg, 71% yield). ECMS calculated for CigbhiBrNsCE (M+H) ⁺ m/z = 434.1; found 434.1.

Step 6: 10-bromo-8-methyl-3-vinyl-4,5-dihydro-3H, 6H-2,2a,5a-triazaaceanthrylen-6-one

To a solution of 5-(9-bromo-7-methyl-5-oxo-3,5-dihydro-4H-pyrazolo[3,4- c]isoquinolin-4-yl)pent-l-en-3-yl ethyl carbonate (209 mg, 0.48 mmol) in 2 mb of THF was added tris(dibenzylideneacetone)dipalladium (88.1 mg, 0.1 mmol) and 1,4- bis(diphenylphosphino)butane (82. 1 mg, 0.19 mmol) at r.t. and degassed with nitrogen three times. After stirring at 60 °C for 30 min, the mixture was diluted with water and extracted with EtOAc. The combined organics were dried with anhydrous sodium sulfate, fdtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with hexane and ethyl acetate to provide the desired product as a white solid (124 mg, 75% yield). LCMS calculated for CieHisBrNsO (M+H) ⁺ m/z = 344.0; found 344.0.

Step 7: 10-acetyl-8-methyl-3-vinyl-4,5-dihydro-3H, 6H-2,2a,5a-triazaaceanthrylen-6-one

A mixture of 10-bromo-8-methyl-3-vinyl-4,5-dihydro-3H,6H-2,2a,5a- triazaaceanthrylen-6-one (124 mg, 0.36 mmol), PdC12(PPh3)2 (51 mg, 0.07 mmol), and tributyl(l-ethoxyvinyl)stannane (260 mg, 0.72 mmol) in dioxane (2 mL) was heated at 100 °C for 2 h under nitrogen atmosphere. Upon cooling to room temperature, the mixture was added 2 N HC1 and stirred for 30 min. Then 200 mg CsF was added and stirred for another 30 min before the mixture was diluted with water and extracted with EtOAc. The combined organics were washed with sat. NaCl and dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with DCM and ethyl acetate to provide the desired product as a white solid (74 mg, 67%). LCMS calculated for C18H18N3O2 (M+H) ⁺ m/z = 308.1; found 308.1.

Step 8: 10-(l-hydroxyethyl)-8-methyl-3-vinyl-4, 5-dihydro-3H, 6H-2, 2a, 5a-triazaaceanthrylen- 6-one

To a mixture of 10-acetyl-8-methyl-3-vinyl-4,5-dihydro-3H,6H-2,2a,5a- triazaaceanthrylen-6-one (74 mg, 0.24 mmol) in 2 mL of THF was added LAH (0.36 mL, 0.36 mmol, 1 M solution in THF) at -78 °C. The resulting mixture was stirred at same temperature for 30 min, and then quenched with sat. NH4CI. The mixture was diluted with water and extracted with EtOAc. The combined organics were washed with sat. NaCl and dried with anhydrous sodium sulfate, fdtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with DCM and ethyl acetate to provide the desired product as a white solid (55 mg, 74%). LCMS calculated for CI ₈ H ₂₀ N ₃ O ₂ (M+H) ⁺ m/z = 310.2; found 310.2.

Step 9: 10-(l-bromoethyl)-8-methyl-3-vinyl-4, 5-dihydro-3H, 6H-2, 2a, 5a-triazaaceanthrylen- 6-one

To a mixture of 10-(l-hydroxyethyl)-8-methyl-3-vinyl-4,5-dihydro-3H,6H-2,2a, 5a- triazaaceanthrylen-6-one (20 mg, 0.06 mmol) in DCM (1 mL) was added PBr ₃ (35 mg, 0.13 mmol). The resulting mixture was stirred at rt for 2 h. The mixture was then diluted with water and extracted with DCM. The combined organics were washed with sat. NaCl, dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was used directly in the next step without further purification.

Step 10: 10-(l-((6-chloro-2-(l-methyl-lH-l,2, 4-triazol-3-yl)pyridin-3-yl)amino)ethyl)-8- methyl-3-vinyl-4, 5-dihydro-3H, 6H-2, 2a, 5a-triazaaceanthrylen-6-one

To a solution of 10-(l-bromoethyl)-8-methyl-3-vinyl-4,5-dihydro-3H,6H-2,2a,5a - triazaaceanthrylen-6-one (23 mg, 0.066 mmol) in 0.6 mL of DMF was added 6-chloro-2-(l- methyl-lH-l,2,4-triazol-3-yl)pyridin-3-amine (26 mg, 0.12 mmol). After stirring at 60 °C for 1 h, the mixture was diluted with water and extracted with EtOAc. The combined organics were dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with hexane and ethyl acetate to provide the desired product as a white solid (28 mg, 90% yield). LCMS calculated for C ₂₆ H ₂₆ C1N ₈ O (M+H) ⁺ m/z = 501.2; found 501.2. Step 11: 10-(l-(( 6-chloro-2-(l -methyl- 1H-1, 2, 4-triazol-3-yl)pyridin-3-yl)amino)ethyl)-8- methyl-6-oxo-4, 5-dihydro-3H, 6H-2, 2a, 5a-triazaaceanthrylene-3-carhaldehyde

To a solution of 10-(l-((6-chloro-2-(l-methyl-lH-l,2,4-triazol-3-yl)pyridin-3 - yl)amino)ethyl)-8-methyl-3-vinyl-4,5-dihydro-3H,6H-2,2a,5a-t riazaaceanthrylen-6-one (28 mg, 0.06 mmol) in 1 mL of THF and 0.5 mL of water was added sodium periodate (48 mg, 0.22 mmol) and Osmium tetroxide (0.07 mL, 0.01 mmol, 4% in water). After stirring at r.t. for 2 h, the mixture was diluted with water and extracted with EtOAc. The combined organics were dried with anhydrous sodium sulfate, fdtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with hexane and ethyl acetate to provide the desired product as a white solid (12 mg, 43% yield). LCMS calculated for C ₂₅ H24C1N ₈ O2 (M+H) ⁺ m/z = 503.2; found 503.2.

Step 12: 10-(l-((6-chloro-2-(l-methyl-lH-l,2, 4-triazol-3-yl)pyridin-3-yl)amino)ethyl)-3- (hydroxymethyl)-8-methyl-4, 5-dihydro-3H, 6H-2, 2a, 5a-triazaaceanthrylen-6-one

To a solution of 10-(l-((6-chloro-2-(l-methyl-lH-l,2,4-triazol-3-yl)pyridin-3 - yl)amino)ethyl)-8-methyl-6-oxo-4,5-dihydro-3H,6H-2,2a,5a-tri azaaceanthrylene-3- carbaldehyde (6 mg, 0.01 mmol) in 0.5 mL of MeOH was added sodium borohydride (1 mg, 0.02 mml) at 0 °C. After stirring at 0 °C for 30 min, the mixture was diluted with water and extracted with EtOAc. The combined organics were dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The resulting mixture was purified by prep- HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5 μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C25H26CIN8O2 (M+H) ⁺ m/z = 505.2; found 505.2.

Examples 285-289.

Examples 285-289 in Table 41 were prepared similarly as described for Example 284. Table 41.

Example 290: 10-(l-((6-Chloro-2-(l-methyl-lH-l,2,4-triazol-3-yl)pyridin-3 - yl)amino)ethyl)-3-((dimethylamino)methyl)-8-methyl-4,5-dihyd ro-3H,6H-2,2a,5a- triazaaceanthrylen-6-one

To a solution of 10-(l-((6-chloro-2-(l-methyl-lH-l,2,4-triazol-3-yl)pyridin-3 - yl)amino)ethyl)-8-methyl-6-oxo-4,5-dihydro-3H,6H-2,2a,5a-tri azaaceanthrylene-3- carbaldehyde (6 mg, 0.01 mmol) in 0.5 mL of ACN and 0.1 mL AcOH was added dimethylamine (0.12 mL, 0.12 mmol, 1 M in THF) and sodium triacetoxyborohydride (7.6 mg, 0.04 mml) at 0 °C. After stirring at r.t. for 30 min, the mixture was diluted with water and extracted with EtOAc. The combined organics were dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The resulting mixture was purified by prep- HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5 μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C27H31CIN9O (M+H) ⁺ m/z = 532.2; found 532.2.

Examples 291-322.

Examples 291-322 in Table 42 were prepared similarly as described for Example 36.

Table 42.

Example 323: 10-(l-((6-Chloro-2-(5-methyl-4,5,6,7-tetrahydropyrazolo[l,5- a]pyrazin-3- yl)pyridin-3-yl)amino)ethyl)-8-methyl-4,5-dihydro-3H,6H-2,2a ,5a-triazaaceanthrylen-6- one

Step 1: tert-butyl 3-(6-chloro-3-((l-(8-methyl-6-oxo-4,5-dihydro-3H,6H-2,2a,5a- triazaaceanthrylen-10-yl)ethyl)amino)pyridin-2-yl)-6, 7-dihydropyrazolo[l,5-a]pyrazine-

5 ( 4H) -carboxylate To a mixture of tert-butyl 3-(3-amino-6-chloropyridin-2-yl)-6,7-dihydropyrazolo[l,5- a|pyrazinc-5(4//)-carboxylatc (intermediate 17) (15.2 mg, 0.04 mmol) and 10-( 1- bromoethyl)-8-methyl-4,5-dihydro-3H,6H-2,2a,5a-triazaaceanth rylen-6-one (Example 36, step 6) (10.0 mg, 0.03 mmol) was added DMF (0.1 mb). The resulting mixture was heated at 60 °C for 1 hour. Mixture was cooled to room temperature, diluted with methanol, purified with by prep-HPLC (column: Sunfire prep 15 C18 column, 30* 150 mm, 5μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product. LCMS calculated for C32H36CIN8O3 (M+H) ⁺ m/z = 615.3; found: 615.3.

Step 2: 10-(l-(( 6-chloro-2-(5-methyl-4, 5, 6, 7-tetrahydropyrazolo[l, 5-a]pyrazin-3-yl)pyridin- 3-yl)amino)ethyl)-8-methyl-4, 5-dihydro-3H, 6H-2, 2a, 5a-triazaaceanthrylen-6-one

At room temperature, tert-butyl 3-(6-chloro-3-((l-(8-methyl-6-oxo-4,5-dihydro- 3H,6H-2,2a,5a-triazaaceanthrylen-10-yl)ethyl)amino)pyridin-2 -yl)-6,7-dihydropyrazolo[l,5- a]pyrazine-5(4H)-carboxylate (4.00 mg , 0.01 mmol) was treated with 1 to 1 mixture of trifluoroacetic acid and dichloromethane. The resulting mixture was stirred at room temperature for 30 minutes. Solvent was removed under reduced pressure. The crude product was added acetonitrile (0.5 mL), acetic acid (0.1 mL), formaldehyde (0.1 mL) and sodium triacetoxyhydroborate (0.82 mg, 3.88 μmol). the resulting mixture was stirred at rt for 2 hours. Upon completion of reaction, the residue was diluted with methanol and purified by prep-HPLC (column: Sunfire prep 15 C18 column, 30* 150 mm, 5μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C28H30CIN8O (M+H)+ m/z = 529.2; found: 529.2;

Example 324: 10-(l-((6-Chloro-6'-((dimethylamino)methyl)-[2,3'-bipyridin] -3- yl)amino)ethyl)-8-methyl-4,5-dihydro-3H,6H-2,2a,5a-triazaace anthrylen-6-one

Step 1 : 10-(l-((2-bromo-6-chloropyridin-3-yl)amino)ethyl)-8-methyl-4 , 5-dihydro-3H, 6H- 2, 2a, 5a-triazaaceanthrylen-6-one

The titled compound was prepared using similar procedures as described for Example 36 with 2-bromo-6-chloropyridin-3-amine replacing 2-aminobenzoic acid in Step 7. The resulting mixture was diluted with water and extracted with EtOAc. The combined organics were washed with sat. NaCl, dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with DCM and ethyl acetate to provide the desired product as a white solid. LCMS calculated for C ₂ iH ₂₍ iBrClbLO (M+H) ⁺ m/z = 472.1; found 472.1.

Step 2: 6-chloro-3-((l-(8-methyl-6-oxo-4, 5-dihydro-3H, 6H-2, 2a, 5a-triazaaceanthrylen-l 0- yl)ethyl)amino)-[2, 3 '-bipyridine] -6'-carbaldehyde

To a solution of 10-(l-((2-bromo-6-chloropyridin-3-yl)amino)ethyl)-8-methyl-4 ,5- dihydro-3H,6H-2,2a,5a-triazaaceanthrylen-6-one (12 mg, 0.03 mmol) in 1.2 mL of toluene and 0.2 mL water was added tetrakis(triphenylphosphine)palladium (5.87 mg, 0.1 mmol), potassium carbonate (10.5 mg, 0.08 mmol) and 5-(4,4,5,5-tetramethyl-l,3,2-dioxaborolan-2- yl)picolinaldehyde (9 mg, 0.04 mmol) under nitrogen. After stirring at 80 °C for 1 h, the mixture was diluted with water and extracted with EtOAc. The combined organics were dried with anhydrous sodium sulfate, fdtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with hexane and ethyl acetate to provide the desired product as a white solid (10 mg, 79% yield). LCMS calculated for C27H ₂ 4C1N ₆ O ₂ (M+H) ⁺ m/z = 499.2; found 499.2.

Step 3: 10-(l-((6-chloro-6'-((dimethylamino)methyl)-[2,3'-bipyridin] -3-yl)amino)ethyl)-8- methyl-4, 5-dihydro-3H, 6H-2, 2a, 5a-triazaaceanthrylen-6-one To a solution of 6-chloro-3-((l-(8-methyl-6-oxo-4,5-dihydro-3H,6H-2,2a,5a- triazaaceanthrylen-10-yl)ethyl)amino)-[2,3'-bipyridine]-6'-c arbaldehyde (5 mg, 0.01 mmol) in 0.5 mb of ACN and 0. 1 mb AcOH was added dimethylamine (0.06 mb, 0.12 mmol, 2 M in THF) and sodium triacetoxyborohydride (7.6 mg, 0.04 mml) at 0 °C. After stirring at r.t. for 30 min, the mixture was diluted with water and extracted with EtOAc. The combined organics were dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The resulting mixture was purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5 μm; mobile phase A: water (0. 1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C29H31CIN7O (M+H) ⁺ m/z = 528.2; found 528.2.

Example 325: 3-(3-((l-(3,8-Dimethyl-6-oxo-4,5-dihydro-3H,6H-2,2a,5a- triazaaceanthrylen-10-yl)ethyl)amino)-6-methylpyridin-2-yl)- l,2,4-oxadiazol-5(4H)-one

To a solution of 3-(6-chloro-3-((l-(3,8-dimethyl-6-oxo-4,5-dihydro-3H,6H-2,2a ,5a- triazaaceanthrylen-10-yl)ethyl)amino)pyridin-2-yl)-l,2,4-oxa diazol-5(4H)-one (Example 70) (5 mg, 0.01 mmol) in 0.5 mL of dioxane and 0. 1 mL water was added methylboronic acid (1.3 mg, 0.02 mmol), potassium phosphate tribasic (6.5 mg, 0.03 mmol) and P(t-Bu)3 Pd G2 (1 mg, 0.002 mmol) under nitrogen. After stirring at 80 °C for 1 h, the mixture was diluted with water and extracted with EtOAc. The combined organics were dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The resulting mixture was purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5 μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C25H26N7O3 (M+H) ⁺ m/z = 472.2; found 472.2.

Example 326: 8-Methyl-10-(l-((6-methyl-2-(2-methyl-2H-tetrazol-5-yl)pyrid in-3- yl)amino)ethyl)-4,5-dihydro-3H,6H-2,2a,5a-triazaaceanthrylen -6-one

The titled compound was prepared using similar procedures as described for Example 325 with 10-(l-((6-chloro-2-(2-methyl-2H-tetrazol-5-yl)pyridin-3-yl)a mino)ethyl)-8-methyl- 4,5-dihydro-3H,6H-2,2a,5a-triazaaceanthrylen-6-one replacing 3-(6-chloro-3-((l-(3,8- dimethyl-6-oxo-4,5-dihydro-3H,6H-2,2a,5a-triazaaceanthrylen- 10-yl)ethyl)amino)pyridin-2- yl)-l,2,4-oxadiazol-5(4H)-one. The resulting mixture was diluted with water and extracted with EtOAc. The combined organics were dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The resulting mixture was purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5 μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C24H26N9O3 (M+H) ⁺ m/z = 456.2; found 456.2.

Example 327 : 3-(3-((l-(3-Ethyl-4,7-dimethyl-5-oxo-4,5-dihydro-3H-pyrazolo [3,4- c]isoquinolin-9-yl)ethyl)amino)-6-methylpyridin-2-yl)-l,2,4- oxadiazol-5(4H)-one

The titled compound was prepared using similar procedures as described for Example 325 with 3-(6-chloro-3-((l-(3-ethyl-4,7-dimethyl-5-oxo-4,5-dihydro-3H -pyrazolo[3,4- c]isoquinolin-9-yl)ethyl)amino)pyridin-2-yl)-l,2,4-oxadiazol -5(4H)-one (Example 59) replacing 3-(6-chloro-3-((l-(3,8-dimethyl-6-oxo-4,5-dihydro-3H,6H-2,2a ,5a- triazaaceanthrylen-10-yl)ethyl)amino)pyridin-2-yl)-l,2,4-oxa diazol-5(4H)-one. The resulting mixture was diluted with water and extracted with EtOAc. The combined organics were dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The resulting mixture was purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5 μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C24H26N7O3 (M+H) ⁺ m/z = 460.2; found 460.2.

Example 328: 10-(l-((6-Chloro-2-(l-methyl-lH-l,2,4-triazol-3-yl)pyridin-3 -yl)amino)-2- hydroxyethyl)-8-methyl-4,5-dihydro-3/7, 6/7-2, 2a, 5a-triazaaceanthrylen-6-one

Step 1: 8-methyl-10-vinyl-4,5-dihydro-3H, 6H-2,2a,5a-triazaaceanthrylen-6-one o YxbO kj. ' ⁼ N

To the compound of 10-bromo-8-methyl-4,5-dihydro-3H,6H-2,2a,5a- triazaaceanthrylen-6-one (Example 36, step 3) (3.7 g, 11.63 mmol) was added Pd(dppf)C12 • DCM (0.85 g, 1.16 mmol), potassium vinyltrifluoroborate (4.67 g, 34.89 mmol) and potassium carbonate (4.82 g, 34.89 mmol) and 5 to 1 mixture of tetrahydrofuran and water (100 mL) at room temperature. The resulting mixture was heated at 70 °C for 2 hours. Upon completion of reaction based on LCMS analysis, mixture was filtered through Celite, filtrate was concentrated under reduced pressure. The residue was purified with silica gel chromatography, eluting from 0% to 100% ethyl acetate/hexanes to give desired product as a brown solid (2.50 g, 81%). LCMS calculated for C16H16N3O (M+H) ⁺ m/z = 266.1; found: 266.1;

Step 2: 8-methyl-l 0-(oxiran-2-yl)-4, 5-dihydro-3H, 6H-2, 2a, 5a-triazaaceanthrylen-6-one

To the solution of 8-methyl- 10-vinyl -4, 5 -dihydro-3 H,6H-2, 2a, 5a-triazaaceanthrylen- 6-one (500 mg, 1.88 mmol) in dichloromethane (18 mL) was added m-chloroperoxybenzoic acid (975 mg, 5.65 mmol) at room temperature. The resulting mixture was stirred at room temperature for 3 hours. Upon completion of reaction based on LCMS analysis, the reaction was quenched with saturated water solution of sodium bicarbonate, organic layer was dried with sodium sulfate. Solvent was removed under reduced pressure to give desired crude product as orange color solid which was used in the next step directly. LCMS calculated for Ci6Hi ₆ N ₃ O ₂ (M+H) ⁺ m/z = 282.1; found: 282.1.

Step 3: 10-(l -((6-chloro-2-(l -methyl-lH-1 ,2, 4-triazol-3-yl)pyridin-3-yl)amino)-2- hydroxyethyl)-8-methyl-4, 5-dihydro-3H, 6H-2, 2a, 5a-triazaaceanthrylen-6-one

To the solution of 8-methyl- 10-(oxiran-2-yl)-4,5-dihydro-3H,6H-2,2a,5a- triazaaceanthrylen-6-one (10.0 mg, 0.04 mmol) in dichloromethane was added scandium(III) triflate (3.5 mg, 0.01 mmol) followed by addition of 6-chloro-2-(l-methyl-lH-l,2,4-triazol-3- yl)pyridin-3 -amine (14.9 mg, 0.07 mmol) at room temperature. The resulting mixture was heated at 60 °C for 2 hours. Upon completion of reaction, the residue was diluted with methanol and purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a yellow solid. LCMS calculated for C24H24CIN8O2 (M+H) ⁺ m/z = 491.2; found: 491.2.

Examples 329-333.

Examples 329-333 in Table 43 were prepared similarly as described for Example 328.

Table 43.

Example 334: 9-(l-((6-Chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3-yl)ami no)-2-(4- methylpiperazin-l-yl)ethyl)-3-ethyl-4,7-dimethyl-3,4-dihydro -5H-pyrazolo[3,4- c]isoquinolin-5-one

Step 1: 9-(l -((6-chloro-2-(l -methyl- lH-pyrazol-4-yl)pyridin-3-yl)amino)-2-hydroxyethyl)-3- ethyl-4, 7-dimethyl-3, 4-dihydro-5H-pyrazolo[3, 4-c]isoquinolin-5-one

The titled compound was prepared using similar procedures as described for Example 76 with 6-chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3-amine replacing 2-bromo-6- chloropyridin-3 -amine in step 4. The crude product was purified by silica gel column chromatography, eluted with DCM and MeOH to provide the desired product as a brown solid. LCMS calculated for C25H27CIN7O2 (M+H) ⁺ m/z = 492.2; found 492.1.

Step 2: 9-(2-((tert-butyldimethylsilyl)oxy)-l-((6-chloro-2-(l-methyl -lH-pyrazol-4-yl)pyridin-

3-yl)amino)ethyl)-3-ethyl-4, 7-dimethyl-3, 4-dihydro-5H-pyrazolo[3, 4-c]isoquinolin-5-one

To a stirred solution of 9-(l-((6-chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3- yl)amino)-2-hydroxyethyl)-3-ethyl-4,7-dimethyl-3,4-dihydro-5 H-pyrazolo[3,4-c]isoquinolin- 5-one (120 mg, 0.24 mmol) in 2 mb DCM was added TBSC1 (45 mg, 0.3 mmol), imidazole (20 mg, 0.3 mmol) at 0 °C. The reaction mixture was stirred at rt for 12h. LCMS analysis indicated the reaction was complete. The reaction was quenched with 30 mb water and extracted with 2 x 30 mb DCM. The combined organics were washed with saturated NaCl, dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted with 0-10% EtOAc in DCM to provide the desired product (130 mg, 90%). LCMS calculated for C3iH4iClN7C>2Si (M+H) ⁺ m/z = 606.3; found 606.3.

Step 3: 9-(l-((6-chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3-yl)(4- methoxybenzyl)amino)-

2-hydroxyethyl)-3-ethyl-4, 7-dimethyl-3, 4-dihydro-5H-pyrazolo[3, 4-c]isoquinolin-5-one

To a mixture of 9-(2-((tert-butyldimethylsilyl)oxy)-l-((6-chloro-2-(l-methyl -lH- pyrazol-4-yl)pyridin-3-yl)amino)ethyl)-3-ethyl-4,7-dimethyl- 3,4-dihydro-5H-pyrazolo[3,4- c]isoquinolin-5-one (130 mg, 0.22 mmol) in DMF (2 mL) was added NaH (12 mg, 0.3 mmol) at 0 °C under nitrogen atmosphere. The resulting mixture was stirred at same temperature for 30 min before 4-methoxybenzyl bromide (60 mg, 0.3 mmol) was added. The reaction mixture was then slowly warmed to rt and stirred for 1 h. After completion, the reaction was carefully quenched with water at 0 °C while vigorously stirred. The mixture was then extracted with ethyl acetate (2 x 30 mL). The combined organics were dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was added 1 mL of THF and tetrabutylammonium fluoride (THF solution, 0.5 mL, 0.5 mmol) at 0 °C. The reaction was stirred at 0 °C for 30 min before quenched with water and extracted with EtOAc. The combined organics were concentrated under reduced pressure. The residue was purified by silica gel column chromatography, eluted DCM/MeOH to provide the desired product (94 mg, 70%). LCMS calculated for C33H35CIN7O3 (M+H) ⁺ m/z = 612.2; found 612.3.

Step 4: 2-((6-chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3-yl)(4-met hoxybenzyl)amino)-2- (3-ethyl-4, 7-dimethyl-5-oxo-4,5-dihydro-3H-pyrazolo[3, 4-c]isoquinolin-9-yl)cicetaldehyde

To a solution of 9-(l-((6-chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3-yl)(4- methoxybenzyl)amino)-2-hydroxyethyl)-3-ethyl-4,7-dimethyl-3, 4-dihydro-5H-pyrazolo[3,4- c]isoquinolin-5-one (94 mg, 0.15 mmol) in 2 mL of DCM was added Dess-Martin periodinane (76 mg, 0.18 mmol) and NaHCCL (50 mg, 0.5 mmoL) at 0 °C. After stirring at r.t. for overnight, the mixture was diluted with water and extracted with EtOAc. The organic layers were dried with anhydrous sodium sulfate, fdtered, and concentrated under reduced pressure. The crude product was used directly in next step without further purification. LCMS calculated for C33H33CIN7O3 (M+H) ⁺ m/z = 610.2; found 610.2.

Step 5: 9-(l-((6-chloro-2-(l -methyl- lH-pyrazol-4-yl)pyridin-3-yl)amino)-2-(4- methylpiperazin-l-yl)ethyl)-3-ethyl-4, 7-dimethyl-3, 4-dihydro-5H-pyrazolo[3, 4-c]isoquinolin- 5-one

To a solution of 2-((6-chloro-2-(l-methyl-lH-pyrazol-4-yl)pyridin-3-yl)(4- methoxybenzyl)amino)-2-(3-ethyl-4,7-dimethyl-5-oxo-4,5-dihyd ro-3H-pyrazolo[3,4- c]isoquinolin-9-yl)acetaldehyde (10.0 mg, 0.016 mmol) in 0.5 mL of DCM was added 1- methylpiperazine (10 uL) and sodium triacetoxyborohydride (4.2 mg, 0.02 mmol). After stirring at r.t. overnight, the mixture was quenched with water and extracted with EtOAc. The combined organics were washed with brine and dried with anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The residue was added 10% TfOH in TFA, the mixture was stirred at rt for 20 min before diluted with MeOH and purified by prep-HPLC (column: Sunfire prep C18 column, 30* 150 mm, 5μm; mobile phase A: water (0.1% TFA), mobile phase B: acetonitrile; flow rate: 60 mL/min); eluted fractions were collected and lyophilized to provide the TFA salt of the desired product as a white solid. LCMS calculated for C30H37CIN9O (M+H) ⁺ m/z = 574.3; found: 574.2.

Example A. pS473 AKT Assay Protocol

SKBR3 (PIK3CA WT), MCF7 (PIK3CA E545K), and T47D (PIK3CA H1047R) cells were cultured using 10-cm petri dishes with recommended medium plus 10% fetal bovine serum. One day before the assay, cells were seeded in 96-well plates at a final density of 10,000 cells per well. After overnight incubation in complete medium, cells were treated with different concentrations of PI3Ka inhibitors for 2 h. Cells were then fixed using 4% paraformaldehyde at room temperature for 20 min. Aspirate 4% paraformaldehyde, and wash cells using IX regular phosphate buffered saline 3 times, 5 min each. Aspirate any residual phosphate buffered saline and block cells using 10% goat serum containing 1% bovine serum albumin and 0.3% Triton X-100 at room temperature for 1 h. Without any additional washing, primary antibodies (rabbit anti-pSer473 AKT) were diluted using blocking buffer and added at a final volume of 50 microliter per well. Keep assay plates with primary antibodies overnight at 4°C. Wash cells using IX regular phosphate buffered saline 3 times, 5 min each. After the final wash, incubate cells with horseradish peroxidase -conjugated secondary antibodies (goat Anti -rabbit IgG) diluted using the same blocking buffer at room temperature for 1 h. Wash cells thoroughly using IX regular phosphate buffered saline 3 times, 5 min each. Aspirate any residual phosphate buffered saline. Add Super-Signal ELISA Pico Chemiluminescent Substrate at a final volume of 100 microliter per well. Read plates on i3x Multi-Mode Microplate Reader and calculate IC50 values using GraphPad Prism software.

Results of the assay described above are presented in Table A. Compounds denoted of the present disclosure showed IC50 values in the following ranges:

A: IC ₅₀ < lOOO nM;

B: 1000 nM < IC50 < 5000 nM;

C: 5000 nM < IC50 < 10000 nM;

D: 10000 nM < IC50 < 30000 nM.

E: 30000 nM < IC50

Table A.

Example B. pS473 AKT Assay Protocol with 2.5% FBS

T47D (PIK3CA H1047R) cells were cultured using 10-cm petri dishes with recommended medium plus 10% fetal bovine serum. One day before the assay, cells were seeded in 96-well plates. After overnight incubation, the medium was changed to DMEM with 2.5% fetal bovine serum (FBS) and the cells were treated with different concentrations of the compounds for 2 h. Cells were then fixed using 4% paraformaldehyde at room temperature for 20 min. Aspirate 4% paraformaldehyde, and wash cells using IX regular phosphate buffered saline 3 times, 5 min each. Aspirate any residual phosphate buffered saline and block cells using 10% goat serum containing 1% bovine serum albumin and 0.3% Triton X-100 at room temperature for 1 h. Without any additional washing, primary antibodies (rabbit anti-pSer473 AKT) were diluted using blocking buffer and added at a final volume of 50 microliter per well. Keep assay plates with primary antibodies overnight at 4°C. Wash cells using IX regular phosphate buffered saline 3 times, 5 min each. After the final wash, incubate cells with horseradish peroxidase -conjugated secondary antibodies (goat Antirabbit IgG) diluted using the same blocking buffer at room temperature for 1 h. Wash cells thoroughly using IX regular phosphate buffered saline 3 times, 5 min each. Aspirate any residual phosphate buffered saline. Add Super-Signal ELISA Pico Chemiluminescent Substrate at a final volume of 100 microliter per well. Read plates on i3x Multi-Mode Microplate Reader and calculate IC50 values using GraphPad Prism software. Results of the assay described above are presented in Tables B-C. “+” indicates an IC50 less than 1000 nM; “++” indicates an IC50 greater than or equal to 1000 nM but less than 5000 nM; “+++” indicates an IC50 greater than or equal to 5000 nM but less than 10000 nM; and “++++” indicates an IC50 greater than or equal to 10000 nM. Table B. Table C.

While we have described a number of embodiments of this invention, it is apparent that our basic examples may be altered to provide other embodiments that utilize the compounds and methods of this invention. Therefore, it will be appreciated that the scope of this invention is to be defined by the appended claims rather than by the specific embodiments that have been represented by way of example.