Title:
INSECT POISON ALLERGENS WITH REDUCED IGE REACTIVITY AND METHOD FOR PRODUCING THE SAME
Document Type and Number:
WIPO Patent Application WO2001040266
Kind Code:
A3
Abstract:
The invention relates to recombinant insect poison allergens and to a specific method for producing them. Said allergens can be varied according to whether they are produced using folds (conformations) that are identical or different to those that occur naturally. The proteins with folds that do not occur naturally have a reduced IgE reactivity or allergenity and can therefore be used as therapeutic agents in the immunotherapy of allergies.
Inventors:
SUCK ROLAND (DE)
CROMWELL OLIVER (DE)
FIEBIG HELMUT (DE)
CROMWELL OLIVER (DE)
FIEBIG HELMUT (DE)
Application Number:
PCT/EP2000/011776
Publication Date:
February 28, 2002
Filing Date:
November 27, 2000
Export Citation:
Assignee:
MERCK PATENT GMBH (DE)
SUCK ROLAND (DE)
CROMWELL OLIVER (DE)
FIEBIG HELMUT (DE)
SUCK ROLAND (DE)
CROMWELL OLIVER (DE)
FIEBIG HELMUT (DE)
International Classes:
A61K38/17; A61K39/00; A61K39/35; A61P37/08; G01N33/53; C07K14/00; C07K14/435; C12N15/09; C12P21/00; C12P21/02; G01N33/68; (IPC1-7): C07K14/435; A61K38/17; G01N33/68; A61P37/08
Other References:
KING T.P. ET AL.: "Murine T and B cell response to natural and recombinant hornet venom allergen Dol m 5.02 and its recombinant fragments", THE JOURNAL OF IMMUNOLOGY, vol. 154, 1995, pages 577 - 584, XP002173770
SUCK R. ET AL.: "Purification and immunobiological characterization of folding variants of the recombinant major wasp allergen ves v 5 (antigen 5)", INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY, vol. 121, 1 April 2000 (2000-04-01), pages 284 - 291, XP001010205
MONSALVE R.I. ET AL.: "Expressions of recombinant venom allergen, antigen 5 of yellowjacket (Vespula vulgaris) and paper wasp (Polistes annularis), in bacteria and yeast", PROTEIN EXPRESSION AND PURIFICATION, vol. 16, August 1999 (1999-08-01), pages 410 - 416, XP002173771
SOLDATOVA L.N. ET AL.: "Superior biologic activity of the recombinant bee venom allergen hyaluronidase expressed in baculovirus-infected insect cells as compared with Escherichia coli", THE JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, vol. 101, no. 5, May 1998 (1998-05-01), pages 691 - 698, XP001014760
FÖRSTER E. ET AL.: "Natural and recombinant enzymatically active or inactive bee venom phospholipase A2 has the same potency to release histamine from basophils in patients with hymenoptera allergy", THE JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, vol. 95, no. 6, June 1995 (1995-06-01), pages 1229 - 1235, XP001014781
SUCK R. ET AL.: "Purification and immunobiological characterization of folding variants of the recombinant major wasp allergen ves v 5 (antigen 5)", INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY, vol. 121, 1 April 2000 (2000-04-01), pages 284 - 291, XP001010205
MONSALVE R.I. ET AL.: "Expressions of recombinant venom allergen, antigen 5 of yellowjacket (Vespula vulgaris) and paper wasp (Polistes annularis), in bacteria and yeast", PROTEIN EXPRESSION AND PURIFICATION, vol. 16, August 1999 (1999-08-01), pages 410 - 416, XP002173771
SOLDATOVA L.N. ET AL.: "Superior biologic activity of the recombinant bee venom allergen hyaluronidase expressed in baculovirus-infected insect cells as compared with Escherichia coli", THE JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, vol. 101, no. 5, May 1998 (1998-05-01), pages 691 - 698, XP001014760
FÖRSTER E. ET AL.: "Natural and recombinant enzymatically active or inactive bee venom phospholipase A2 has the same potency to release histamine from basophils in patients with hymenoptera allergy", THE JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, vol. 95, no. 6, June 1995 (1995-06-01), pages 1229 - 1235, XP001014781
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