Title:
INTEGRATIVE PLASMID
Document Type and Number:
WIPO Patent Application WO/2022/097647
Kind Code:
A1
Abstract:
The present disclosure pertains to production of a virus vector plasmid. According to one aspect, the present disclosure provides a method for producing a virus vector plasmid having a sequence to be replicated in Bacillus subtilis. The method includes a step for forming a plasmid in a host cell by introducing, into the host cell, a nucleic acid that has a sequence to be replicated in Bacillus subtilis and that includes a nucleic acid sequence for producing a virus vector. In one embodiment, Bacillus subtilis could have the ability to form a plasmid from a nucleic acid acquired from outside, and therefore, in this method, the nucleic acid introduced dose not have be a plasmid.
Inventors:
SAITO SHUNSUKE (JP)
TSUGE KENJI (JP)
TSUGE KENJI (JP)
Application Number:
PCT/JP2021/040411
Publication Date:
May 12, 2022
Filing Date:
November 02, 2021
Export Citation:
Assignee:
SYNPLOGEN CO LTD (JP)
International Classes:
C12N15/86; C12N1/21; C12N7/01; C12N15/34; C12N15/35; C12N15/75
Domestic Patent References:
| WO2001090392A1 | 2001-11-29 | |||
| WO2015111248A1 | 2015-07-30 |
Foreign References:
| JP2002528049A | 2002-09-03 | |||
| US5908845A | 1999-06-01 | |||
| JP2018093024A | 2018-06-14 | |||
| JP2020184491A | 2020-11-12 |
Other References:
ISSAY NARUMT ET AL., BIOTECHNOLOGY LETTERS, vol. 17, no. 5, May 1995 (1995-05-01), pages 475 - 480
MOLEKULIARNAIA GENETIKA, no. 5, 1 May 1991 (1991-05-01), pages 26 - 29
P TRIEU-CUOT ET AL., EMBO J., vol. 4, no. 13A, 16 December 1985 (1985-12-16), pages 3583 - 3587
JANNIERE, L.A. GRUSSS. D. EHRLICH: "Plasmids", 1993, AMERICAN SOCIETY FOR MICROBIOLOGY, article "Bacillus subtilis and other gram-positive bacteria: biochemistry, physiology and molecular genetics", pages: 625 - 644
AUSUBEL ET AL.: "Current Protocols in Molecular Biology", 1995, WILEY INTERSCIENCE PUBLISHERS, article "DNA Cloning 1: Core Techniques, A Practical Approach"
SAMBROOK, J. ET AL.: "Molecular Cloning: A Laboratory Manual", vol. 1, 1989, COLD SPRING HARBOR LABORATORY PRESS, pages: 42 - 45
IMANAKA, T. ET AL., J. GEN. MICROBIOI., vol. 130, 1984, pages 1399 - 1408
TANAKA, TOGRA, M., FEBS LETT., vol. 422, 1998, pages 243 - 246
SWINFIELD, T. J. ET AL., GENE, vol. 87, 1990, pages 79 - 90
YANSURA, D.HENNER, D., J. PRO. NATL. ACAD. SCI, USA, vol. 81, 1984, pages 439 - 443
ITAYA, M., BIOSCI. BIOTECHNOL. BIOCHEM., vol. 63, 1999, pages 602 - 604
ANAGNOSTOPOULOU, C.SPIZIZEN, J., J. BACTERIOL., vol. 81, 1961, pages 741 - 746
IMANAKA, T. ET AL., J.GEN.MICROBIOI, vol. 130, 1984, pages 1399 - 1408
JOURNAL OF BACTERIOLOGY, vol. 183, pages 5453 - 5458
TSUGE, K.SATO, Y.KOBAYASHI, Y.GONDO, M.HASEBE, M.TOGASHI, T.TOMITA, M.ITAYA, M.: "Method of preparing an equimolar DNA mixture for one-step DNA assembly of over 50 fragments", SCIENTIFIC REPORTS, vol. 5, 2015, pages 10655, XP055369781, DOI: 10.1038/srep10655
MOLEKULIARNAIA GENETIKA, no. 5, 1 May 1991 (1991-05-01), pages 26 - 29
P TRIEU-CUOT ET AL., EMBO J., vol. 4, no. 13A, 16 December 1985 (1985-12-16), pages 3583 - 3587
JANNIERE, L.A. GRUSSS. D. EHRLICH: "Plasmids", 1993, AMERICAN SOCIETY FOR MICROBIOLOGY, article "Bacillus subtilis and other gram-positive bacteria: biochemistry, physiology and molecular genetics", pages: 625 - 644
AUSUBEL ET AL.: "Current Protocols in Molecular Biology", 1995, WILEY INTERSCIENCE PUBLISHERS, article "DNA Cloning 1: Core Techniques, A Practical Approach"
SAMBROOK, J. ET AL.: "Molecular Cloning: A Laboratory Manual", vol. 1, 1989, COLD SPRING HARBOR LABORATORY PRESS, pages: 42 - 45
IMANAKA, T. ET AL., J. GEN. MICROBIOI., vol. 130, 1984, pages 1399 - 1408
TANAKA, TOGRA, M., FEBS LETT., vol. 422, 1998, pages 243 - 246
SWINFIELD, T. J. ET AL., GENE, vol. 87, 1990, pages 79 - 90
YANSURA, D.HENNER, D., J. PRO. NATL. ACAD. SCI, USA, vol. 81, 1984, pages 439 - 443
ITAYA, M., BIOSCI. BIOTECHNOL. BIOCHEM., vol. 63, 1999, pages 602 - 604
ANAGNOSTOPOULOU, C.SPIZIZEN, J., J. BACTERIOL., vol. 81, 1961, pages 741 - 746
IMANAKA, T. ET AL., J.GEN.MICROBIOI, vol. 130, 1984, pages 1399 - 1408
JOURNAL OF BACTERIOLOGY, vol. 183, pages 5453 - 5458
TSUGE, K.SATO, Y.KOBAYASHI, Y.GONDO, M.HASEBE, M.TOGASHI, T.TOMITA, M.ITAYA, M.: "Method of preparing an equimolar DNA mixture for one-step DNA assembly of over 50 fragments", SCIENTIFIC REPORTS, vol. 5, 2015, pages 10655, XP055369781, DOI: 10.1038/srep10655
Attorney, Agent or Firm:
YAMAMOTO, Shusaku et al. (JP)
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