Title:
INTELLIGENT AND CONTROLLABLE MICROBIAL DRUG FACTORY AND USE THEREOF IN SKIN WOUND HEALING
Document Type and Number:
WIPO Patent Application WO/2023/185443
Kind Code:
A1
Abstract:
Disclosed are an intelligent and controllable microbial drug factory and use thereof in skin wound healing, said microbial drug factory comprising a gene expression loop control system and a chassis probiotic. Also disclosed are a glucose-regulated gene expression loop control system and use thereof in regulating and controlling blood glucose, said control system comprising a transcriptional repressor HexR, a glucose-induced strong promoter, and a sequence to be transcribed. The glucose-regulated gene expression loop control system of the present invention has kinetic characteristics of fine-tuning and reversible expression. Further disclosed are a prokaryotic expression vector, an engineered cell and use thereof in drugs for treating diabetes, promoting skin wound healing, and preventing and/or treating metabolic diseases. The present invention relates to the fields of synthetic biology and cell therapy, combines microbial synthetic biology with disease treatment, and has a wide range of application prospects.
Inventors:
YE HAIFENG (CN)
GUAN NINGZI (CN)
GAO XIANYUN (CN)
GUAN NINGZI (CN)
GAO XIANYUN (CN)
Application Number:
PCT/CN2023/081260
Publication Date:
October 05, 2023
Filing Date:
March 14, 2023
Export Citation:
Assignee:
UNIV EAST CHINA NORMAL (CN)
International Classes:
C12N1/21; C12N15/70; C12R1/125; C12R1/19; C12R1/25
Foreign References:
| US20210163963A1 | 2021-06-03 | |||
| CN103370410A | 2013-10-23 | |||
| US20190338376A1 | 2019-11-07 | |||
| US20190367865A1 | 2019-12-05 |
Other References:
KIM JUHYUN, JEON CHE OK, PARK WOOJUN: "Dual regulation of zwf-1 by both 2-keto-3-deoxy-6-phosphogluconate and oxidative stress in Pseudomonas putida", MICROBIOLOGY, SOCIETY FOR GENERAL MICROBIOLOGY, READING, vol. 154, no. 12, 1 December 2008 (2008-12-01), Reading , pages 3905 - 3916, XP093094240, ISSN: 1350-0872, DOI: 10.1099/mic.0.2008/020362-0
FAN RONG; LIU JINLEI; HAN WUYANG; XU MEIXUE;: "Study of the New Inducible Promoter in Corynebacterium Glutamicum", JOURNAL OF HAINAN NORMAL UNIVERSITY (NATURAL SCIENCE), vol. 29, no. 02, 31 December 2016 (2016-12-31), pages 154 - 159, XP009548977, ISSN: 1674-4942
FAN RONG; LIU JINLEI; HAN WUYANG; XU MEIXUE;: "Study of the New Inducible Promoter in Corynebacterium Glutamicum", JOURNAL OF HAINAN NORMAL UNIVERSITY (NATURAL SCIENCE), vol. 29, no. 02, 31 December 2016 (2016-12-31), pages 154 - 159, XP009548977, ISSN: 1674-4942
Attorney, Agent or Firm:
MSJLAW OFFICE (CN)
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