Title:
ISOLATED DNA ENCODING ENZYME FOR PHAGE RESISTANCE ISOLATED DNA ENCODING ENZYME FOR PHAGE RESISTANCE
Document Type and Number:
WIPO Patent Application WO1996021017
Kind Code:
A3
Abstract:
An isolated DNA of a Lactococcus lactis showing a SEQ ID NO: 1 encoding a restriction and twp modification enzymes (R/M SEQ ID NO: 2, 3 and 4). The isolated DNA is used to transform sensitive dairy cultures, such as Lactococcus lactis and Streptococcus thermophilus, to provide phage resistance. Escherichia coli can be used to produce endonucleases.
Inventors:
MOINEAU SYLVAIN (CA)
WALKER SHIRLEY A (CA)
VEDAMUTHU EBENEZER R (CA)
VANDENBERGH PETER A (CA)
WALKER SHIRLEY A (CA)
VEDAMUTHU EBENEZER R (CA)
VANDENBERGH PETER A (CA)
Application Number:
PCT/NL1995/000448
Publication Date:
November 21, 1996
Filing Date:
December 29, 1995
Export Citation:
Assignee:
QUEST INT (NL)
MOINEAU SYLVAIN (NL)
WALKER SHIRLEY A (NL)
VEDAMUTHU EBENEZER R (NL)
VANDENBERGH PETER A (NL)
MOINEAU SYLVAIN (NL)
WALKER SHIRLEY A (NL)
VEDAMUTHU EBENEZER R (NL)
VANDENBERGH PETER A (NL)
International Classes:
A23C9/123; A23C19/032; C12N1/21; C12N9/10; C12N15/09; C12N15/31; C12R1/46; (IPC1-7): C12N15/31; C12N15/54; C12N15/55; C07K14/315; C12N9/22; C12N9/10; C12N15/75; C12N1/20; A23C9/123
Foreign References:
| EP0316677A2 | 1989-05-24 | |||
| US4883756A | 1989-11-28 |
Other References:
UENO, T. ET AL.: "Gene structure and expression of the MboI restriction-modification system", NUCLEIC ACIDS RESEARCH, vol. 21, no. 10, 25 May 1993 (1993-05-25), pages 2309 - 2313, XP002005772
MANNARELLI, B.M. ET AL.: "Nucleotide sequence of the Dpn II DNA methylase gene of Streptococcus pneumoniae and its relationship to the dam gene of Escherichia coli", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, vol. 82, July 1985 (1985-07-01), pages 4468 - 4472, XP002005773
NYENGAARD, N. ET AL.: "Restriction-modification system in Lactococcus lactis", GENE, vol. 157, 22 May 1995 (1995-05-22), pages 13 - 18, XP002005774
NYENGAARD, N. ET AL.: "LlaAI and LlaBI, two type-II restriction endonucleases from Lactococcus lactis subsp. cremoris W9 and W56 recognizing, respectively, 5'-/GATC-3' and 5'-C/TRYAG-3'", GENE, vol. 136, 1993, pages 371 - 372, XP002005775
KLAENHAMMER, T.R.: "Development of bacteriophage-resistant strains of lactic acid bacteria", BIOCHEMICAL SOCIETY TRANSACTIONS, vol. 19, no. 3, August 1991 (1991-08-01), pages 675 - 681, XP002005776
MOINEAU, S. ET AL.: "Restriction/modification systems and restriction endonucleases are more effective on lactococcal bacteriophages that have emerged recently in the dairy industry", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 59, no. 1, January 1993 (1993-01-01), pages 197 - 202, XP002005777
MERCENIER, A.: "Molecular genetics of Streptococcus thermophilus", FEMS MICROBIOLOGY REVIEWS, vol. 87, 1990, pages 61 - 78, XP002014350
MOLLET, B. ET AL.: "Molecular genetics in Streptococcus thermophilus: from transformation to gene expression", LAIT, vol. 73, 1993, pages 175 - 180, XP002014351
MOINEAU, S. ET AL.: "Cloning and sequencing of LlaII restriction/modification genes from Lactococcus lactis and relatedness of this system to the Streptococcus pneumoniae DpnII system", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 61, no. 6, June 1995 (1995-06-01), pages 2193 - 2202, XP002005778
MOINEAU, S. ET AL.: "Expression of a Lactococcus lactis phage resistance mechanism by Streptococcus thermophilus", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 61, no. 7, July 1995 (1995-07-01), pages 2461 - 2466, XP002014352
MANNARELLI, B.M. ET AL.: "Nucleotide sequence of the Dpn II DNA methylase gene of Streptococcus pneumoniae and its relationship to the dam gene of Escherichia coli", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, vol. 82, July 1985 (1985-07-01), pages 4468 - 4472, XP002005773
NYENGAARD, N. ET AL.: "Restriction-modification system in Lactococcus lactis", GENE, vol. 157, 22 May 1995 (1995-05-22), pages 13 - 18, XP002005774
NYENGAARD, N. ET AL.: "LlaAI and LlaBI, two type-II restriction endonucleases from Lactococcus lactis subsp. cremoris W9 and W56 recognizing, respectively, 5'-/GATC-3' and 5'-C/TRYAG-3'", GENE, vol. 136, 1993, pages 371 - 372, XP002005775
KLAENHAMMER, T.R.: "Development of bacteriophage-resistant strains of lactic acid bacteria", BIOCHEMICAL SOCIETY TRANSACTIONS, vol. 19, no. 3, August 1991 (1991-08-01), pages 675 - 681, XP002005776
MOINEAU, S. ET AL.: "Restriction/modification systems and restriction endonucleases are more effective on lactococcal bacteriophages that have emerged recently in the dairy industry", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 59, no. 1, January 1993 (1993-01-01), pages 197 - 202, XP002005777
MERCENIER, A.: "Molecular genetics of Streptococcus thermophilus", FEMS MICROBIOLOGY REVIEWS, vol. 87, 1990, pages 61 - 78, XP002014350
MOLLET, B. ET AL.: "Molecular genetics in Streptococcus thermophilus: from transformation to gene expression", LAIT, vol. 73, 1993, pages 175 - 180, XP002014351
MOINEAU, S. ET AL.: "Cloning and sequencing of LlaII restriction/modification genes from Lactococcus lactis and relatedness of this system to the Streptococcus pneumoniae DpnII system", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 61, no. 6, June 1995 (1995-06-01), pages 2193 - 2202, XP002005778
MOINEAU, S. ET AL.: "Expression of a Lactococcus lactis phage resistance mechanism by Streptococcus thermophilus", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 61, no. 7, July 1995 (1995-07-01), pages 2461 - 2466, XP002014352
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