“ISOLATING HOST SPECIFIC EFFICIENT RHIZOBIUM SPECIES AND METHOD EMPLOYED THEREOF" DESCRIPTION TECHNICAL FIELD [001] The present disclosure generally relates to nitrogen-fixing soil bacteria. More particularly, the present invention relates to the method of identifying, isolating and authentication of host specific efficient Rhizobium species from Homogenous Soil Mixture (HSM) for the preparation of efficient bioinoculant specific to legume plants, more specifically Vigna radiata (green gram) to improve problematic soils with poor nutrition thereby enhancing soil fertility, nodulation and crop growth. BACKGROUND [002] Microorganisms are the central hub of nutrient cycling in the nature. They play an important role in providing nutrients to the plants. This is accomplished by the abilities like Biological Nitrogen fixation (BNF), phosphate solubility, siderophore production, IAA production, etc. of microorganisms. Consequently, soil fertility largely depends upon its microbial population and their activity. BNF is the process that occurs in both independent and symbiotic association. The most popular one is the symbiotic association found in between legume and a Rhizobium species. Legumes are well known for its symbiotic association with Rhizobium for BNF, which further adds to soil nitrogen availability. For instance, groundnut has the potential to fix atmospheric nitrogen at the rate of 21 to 206 kg/ha annually in soils through root nodule bacterium belonging to the genus Bradrhizobium, thus improves soil. Symbiosis between leguminous plants and rhizobia is of considerable agricultural importance. [003] These nitrogen-fixing soil bacteria, which directly or indirectly stimulate the plant growth, have been targeted as the potential plant growth promoting bacteria. The strains of Rhizobium reportedly have the potential to be used as PGPR with non-legumes like corn, rape, wheat, radish, maize, barley and rice. Rhizobial strains which produce siderophore and solubilize inorganic phosphate are further useful in plant growth promotion. Outstanding properties of Rhizobium species made them to be a potential bio-fertilizer/ PGPR. Even Rhizobia were the first microbial inoculants identified and used commercially for improving productivity in legumes. Rhizobium infection to the legume plants is affected by several factors like soil type, pH, moisture, and climate. In other words, geo-climatic conditions affect the interactions of Rhizobium and legume plants; Vigna radiata (green gram) [004] Nitrogen fixing ability of the legume plant-Rhizobium interaction is generally associated with the number of nodule, and dry weight of nodule and plant. As reported earlier, estimating the total N accumulated in legume plants is one of the best parameters to measure N fixation under experimental condition. Therefore, in this study, specific attention is focused on the dry weights of nodule and the plant, nodule number, plant height, and total N and Chlorophyll contents of treated and control plants. Isolated strain of Rhizobium causes a significant increase in nodule development (dry weight and number of nodules) and some growth parameters (dry weights of root and shoot) of the plants under study. Increased nodule number relates positively to the dry weights of the nodules on green gram. [005] An efficient Rhizobium is a species that is able to compete in the field with other indigenous rhizobia for the colonization of the rhizosphere of its homologous legume partner, under various soil physical and chemical conditions. This efficient strain will form many large nitrogen-fixing nodules on the roots of the plant host that will supply, for most legumes, from 70% to 90% of the plant need in nitrogen. Inoculations of Rhizobium species causes a greater increase in growth and yield and the number of nodules per root system is significantly higher in plants inoculated with Rhizobium species compared to plants without Rhizobium species under field condition. In addition to their beneficial N ₂ - fixing activity with legumes, Rhizobium can improve plant P nutrition by mobilizing inorganic and organic P. In the light of aforementioned discussion, there exists a need identify host specific efficient Rhizobium species for nitrogen fixation in problematic soils. SUMMARY [006] The following presents a simplified summary of the disclosure in order to provide a basic understanding to the reader. This summary is not an extensive overview of the disclosure and it does not identify key/critical elements of the invention or delineate the scope of the invention. Its sole purpose is to present some concepts disclosed herein in a simplified form as a prelude to the more detailed description that is presented later. [007] Exemplary embodiments of the present disclosure are directed towards a method for identifying and authentication of efficient host- specific Rhizobium species for the preparation of potent Rhizobium bioinoculant from Homogenous Mixture of Soil (HMS) by cultivatable method and the process comprises of the following steps: a. Collecting a number of forest soil samples from various undisturbed forest areas; b. Mixing a number of forest soil samples to form a single Homogenous Mixture of Soil (HMS); c. Sowing seeds of the desired leguminous plant in the Homogenous Mixture of Soil(HMS) and growing the seeds into the desired leguminous plant under suitable conditions in the suitable container for a predetermined period; d. Selecting and separating the best supporting root nodules from one of the desired leguminous plant, which exhibits desired growth parameters and presence of large proportion of root nodules; e. Washing the best supporting root nodules with either water or diluted ethanol or both for inoculation; f. Inoculating the obtained root nodule onto a sterile YEMA medium plates and incubating the Petri plates under aseptic conditions for isolating the Rhizobium strain ; g. Collecting the Rhizobium strain from the bacterial colonies for isolating the efficient Rhizobium species; h. Identifying and authentication of efficient Rhizobium species. [008] An objective of the present disclosure is directed towards identification of host specific efficient Rhizobium species from Homogenous Mixture of Soils (HMS) for preparation of potent bioinoculant. [009] Another objective of the present disclosure is directed towards fixing atmospheric nitrogen in problematic soils. [0010] Another objective of the present disclosure is directed towards to exploring the forest soils to get a potent Rhizobium species specific to Vigna radiata (green gram). [0011] Another objective of the present disclosure is directed towards improving growth parameters of the legume plants. [0012] Another objective of the present disclosure is directed to increasing nodule development, dry weight and number of nodules. [0013] Another objective of the present disclosure is directed towards isolation, screening, and preparation of the best efficient Rhizobium bio inoculants in a host specific manner from soil samples collected from the unexplored forest rhizosphere soils. [0014] Another objective of the present disclosure is directed towards identifying and grading the efficient strain among the isolates. [0015] Another objective of the present disclosure is directed towards selection of an efficient Rhizobium species apt to the specific locality. [0016] According to an exemplary aspect, methods for preparation and application of host- specific efficient Rhizobium bioinoculant for enhancing soil fertility, improved nodulation and leguminous crop growth are disclosed. [0017] According to another exemplary aspect, host-specific efficient Rhizobium bioinoculants prepared by employing best supporting root nodules from leguminous plants is disclosed. [0018] According to another exemplary embodiment, the present disclosure is on exploring the forest soils to get a potent Rhizobium specific to Vigna radiata (green gram) This method aims at isolation, screening, and preparation of the best efficient Rhizobium bio inoculants in a host specific manner from soil samples collected from the unexplored forest rhizosphere soils. To identify and grade the efficient strain among the isolates, in the present study, the parameters like Nodule size, amount of leghaemoglobin, N-content, chlorophyll content, fresh weight, length of the plant and dry weight of nodule and plant are considered. This method favours the plant by affording several Rhizobium strains available in the source sample; under the geo-climatic condition of the specific place. Thus, favours in selection of an efficient Rhizobium species apt to the said locality. BRIEF DESCRIPTION OF THE DRAWINGS [0019] In the following, numerous specific details are set forth to provide a thorough description of various embodiments. Certain embodiments may be practiced without these specific details or with some variations in detail. In some instances, certain features are described in less detail so as not to obscure other aspects. The level of detail associated with each of the elements or features should not be construed to qualify the novelty or importance of one feature over the others. [0020] FIG. 1 is a flow chart depicting a method for identifying, isolating and authentication of efficient host- specific Rhizobial species using Homogenous Mixture of Soil (HMS) by cultivatable method, in accordance with one or more exemplary embodiments of the present disclosure. [0021] Fig. 2 is a flow chart depicting a process of identification of host-specific Rhizobium strain, in accordance with one or more exemplary embodiments of the present disclosure. [0022] FIG.3is a flow chart depicting a method for authentication of Rhizobium, in accordance with one or more exemplary embodiments of the present disclosure. [0023] FIG. 4 is a flowchart depicting a method of isolating of efficient Rhizobium species available in the Homogenous Soil Mixture (HSM), in accordance with one or more exemplary embodiments of the present disclosure. [0024] FIG.5A is a diagrammatic representation of process of preparation of HMS for growing host specific Rhizobium root nodules, in accordance with one or more exemplary embodiments of the present disclosure. [0025] FIG 5B is a diagrammatic representation of process of selecting the best triplicates of Vigna radiata (green gram), in accordance with one or more exemplary embodiments of the present disclosure. [0026] FIG. 6 is a diagrammatic representation of method of isolation of Rhizobium species, in accordance with one or more exemplary embodiments of the present disclosure. [0027] FIG.7 is schematic representation of phylogenetic tree of Rhizobia present in the Homogenous mixture of all collected Rhizosphere Soil, in accordance with one or more exemplary embodiments of the present disclosure. [0028] FIG.8 is a schematic representation of phylogenetic tree identifying Rhizobium species WG MH290562.1 (Culture deposition number MTCC 12969 &; JCM 33803). DETAILED DESCRIPTION OF EXAMPLE EMBODIMENTS [0029] It is to be understood that the present disclosure is not limited in its application to the details of construction and the arrangement of components set forth in the following description or illustrated in the drawings. The present disclosure is capable of other embodiments and of being practiced or of being carried out in various ways. Also, it is to be understood that the phraseology and terminology used herein is for the purpose of description and should not be regarded as limiting. [0030] The use of “including”, “comprising” or “having” and variations thereof herein is meant to encompass the items listed thereafter and equivalents thereof as well as additional items. The terms “a” and “an” herein do not denote a limitation of quantity, but rather denote the presence of at least one of the referenced item. Further, the use of terms “first”, “second”, and “third”, and the like, herein do not denote any order, quantity, or importance, but rather are used to distinguish one element from another. [0031] Referring to FIG.1 is a flow chart 100 depicting the method for identifying, isolating and authentication of efficient host-specific Rhizobial species using Homogenous Mixture of Soil (HMS) by cultivatable method. [0032] The method commences at step 101, collecting a number of forest soil samples from different forest areas. At step 103, mixing a number of forest soil samples to form a single homogenous mixture of soil (HMS). Thereafter at step 105, sowing seeds of the desired leguminous plant in the homogenous mixture of soil (HMS) and growing the seeds into the desired leguminous plant under suitable conditions in the suitable container for a predetermined period, in the present disclosure the desired leguminous plant is Vigna radiata (green gram). At step 107, selecting and separating the best supporting root nodules from one of the desired leguminous plant, which exhibits desired growth parameters and presence of large proportion of root nodules. Thereafter at step 109, washing the best supporting root nodules with either water or diluted ethanol or both for inoculation. At step 111, inoculating the obtained root nodule onto sterile YEMA medium plates and incubating the Petri plates under aseptic conditions for isolating the Rhizobium strain. At step 113, collecting the Rhizobium from the bacterial colonies for isolating the efficient Rhizobium species and at step 115, identifying and authentication of efficient Rhizobium species. [0033] Referring to Fig. 2 is a flow chart depicting the process of identification of host-specific Rhizobia strain, in accordance with one or more exemplary embodiments of the present disclosure. The method 200 may be carried out in the context of the details of FIG.1. Further, the aforementioned definitions may equally apply to the description below. [0034] The method commences at step 202, collecting soils from six districts (Adilabad, Karimnagar, Khammam, Mahabubnagar, Medak and Warangal) for sampling process, considering 3 different forests from each district (3x6=18) and soils from 9 sites of each forest (9x18=162) total of 162 samples are packed in a sterile zip-lock covers and brought to the lab. Thereafter at step 204, mixing the 162 samples to form a single Homogenous Soil Mixture (HSM) sample; the homogenous mixture of rhizospheric soils being specific to each different plant. Therefore, when a mixture of samples are added to different plants, only one type of host specific Rhizobium strain effects the host forming large root nodules with a tremendous increase in the plants root and shoot height and also increased nitrogen fixation. At step 206, selecting undamaged and healthy Vigna radiata (green gram) seeds of proper shape and size from a single lot for sowing. Thereafter, at step 208, sterilizing the surface of seeds firstly with 0.1% HgCl ₂ solution and then washing with sterile distilled water for 5 times to remove the traces of HgCl ₂ . At step 210, implanting 5 seeds in each pot with Homogenous Soil Mixture (HSM), later thinning to one. At step 212, irrigating the pots regularly and giving Hoagland’s solution course now and then, also maintaining the triplicates. At 214, sowing and extracting after 60 days the best grown plants in terms of length, weight and health and washing the roots with jet of water to remove adhering dirt/soil particles. Thereafter at step 216, selecting the root nodules of large size, pink in color, similar in shape and know to be efficient. Hereby, at step 218 taking the surface sterilized root nodules into a sterile tube containing YEM broth and crushing the root nodules with a sterile glass rod for here care is to be taken that test-tube must not break At step 220, inoculating the obtained root nodule extract onto a sterile YEMA medium plates by spread plate method. At step 222, incubating the Petri plates at room temperature for 3-5 days under aseptic conditions until visible colonies of Rhizobium are developed. At step 224, collecting the Rhizobium sample for conducting root nodule study .The confirmatory tests are performed using the source sample for identifying the Rhizobium species from the Homogenous Soil Mixture (HSM), the confirmatory tests are explained in detail in the following embodiments. [0035] In an exemplary embodiment, confirmatory tests are carried out for the identification of Rhizobium species from the Homogenous Soil Mixture (HSM) the Congo red assists the recognition of Rhizobium amongst other kinds of bacteria. In general the Rhizobium absorbs the dye weekly many of the common soil bacteria take it up strongly. In the present disclosure the Homogenous Soil Mixture (HSM), containing Rhizobial strain bacteria takes it up weekly confirming presence of the Rhizobium species [0036] In another exemplary embodiment, the Rhizobium sample is observed for growth in alkaline medium. According to the test YEMA is added with 1 ml/lit of thymol blue (1.6% sol.) and is adjusted to pH 11 with (approximately 28 m/N NaOH) on slants, the change in colour of indicator is observed up to 15 days. No growth or change in colour is observed, confirming Rhizobium species. [0037] In another embodiment, the Rhizobium sample is observed for growth in Glucose - peptone agar media (Glucose 10 g, peptone 20.0 g NaCl - 5.0, Agar - 15.0, Bromo cresol purple 1.0 ml (1.6% alcoholic sol.) pH 7.1) is used to differentiate rhizobia, which shows little or no growth on the media without altering the pH of the media, observations are taken after 15 days of incubation for growth and change in pH. [0038] In an exemplary embodiment, nodulation tests are conducted by ‘Agar tube method’ this method is to study the nodulation and differentiation of symbiotic effectiveness with plants having small seeds. In this method the plants are wholly enclosed within the glass tube. Sufficient JSA medium (1.0 g CaHPO4, 0.2 g K2HPO4, 0.2 g MgSO4.7H2O, 0.2 g NaCl, 0.1 g FeCl3¬, 8-15 g Agar, 1 litre Distilled water, (1 ml/litre trace element solution) ) is added to the medium (15 ml for deep and 20 ml for slope) and put in the tubes (200 mm x 25 mm). The tubes are closed with cotton plugs of uniform depth (20 mm) and moderate compactness and the tubes are autoclaved and set as agar deep tubes or slopes as required. Nitrogen supplied control and Nitrogen-deficient control are prepared Nitrogen supplied control: Nitrogen-controls are provided to a final concentration of approximately 70 ppm N (0.05% KNO3). Nitrogen-deficient control: Agar tubes without inoculation are planted with seed or pre-germinated seedlings and put as un-inoculated N-deficient control. [0039] In another exemplary embodiment, explains the root nodule study, the white nodules are not considered for the study as they are under developed and are formed due to wrong bacteria and do not fix nitrogen. Pink or rusty coloured nodules are active and effective in N2 fixing. Effective nodules change to green under unfavourable conditions like nutrient deficiency, disease or water stress, etc. and revert back after conditions become normal if not results in black i.e. nodule is dead. (Cumming et al. 2014) Relatively large, pink and efficient root nodules are isolated from 4 plants and physical parameters are assessed and tabulated.The isolate obtained in the present disclosure, fulfils the criteria to be accepted as an efficient Rhizobium for the plant under study Vigna radiata (green gram). Further, all the possible combinations of the potential rhizobial isolate are tested under field conditions for evaluating the improvement of nodulation and growth in these plants through inoculation in field trials for the unravelling and amelioration of crop production in barren, polluted and agricultural soils. The results showed significant increase in growth and nodulation. The nodulation ability of the isolate is confirmed by inoculation tests. It is observed that the identified Rhizobium species played an important role in the growth of plants by showing significant increase in nodulation properties even in barren and polluted soils from the tabulated forms (Table 4, Fig.3). The same Rhizobium species showed host specificity with the same legume tree species hence proving the need of the experimentation and this method can be applied globally for any kind of legume plant or tree species. Table 1: Physical parameters of Nodules in Vigna radiata (green gram) grown in HMS Table 2: Physical parameters of the plants grown in HMS.

Table 3: Nodulation score chart. Table 4: Lab plants results growth of Vigna radiata (green gram) after 45 days [0040] Referring to FIG.3 is a flow chart depicting a method for authentication and isolating of host- specific Rhizobium, in accordance with one or more exemplary embodiments of the present disclosure. The method 300 may be carried out in the context of the details of FIG.1.FIG.2. Further, the aforementioned definitions may equally apply to the description below. [0041] The method commences, growing of seedlings for authentication of Rhizobium species. At step 301, taking a sterile screw capped container of 50ml and filling it with substratum (sterilized soil) supporting the growth of green gram. Thereafter, at step 303, placing surface sterilized seeds into the soil, hereby at step 305, covering the base with aluminium foil for protecting the roots from light. At step 307, place the container near the window by allowing sufficient sunlight for photosynthesis to take place. At step 309, inoculate with the Rhizobium after seed germination (say about 2-3 days) at this step the control is maintained without inoculating the Rhizobium. Thereafter, at step 311, the pots are regularly irrigated with sterile water and now and then with sterile Hoagland’s solution which is a hydroponic nutrient solution. At step 313, the plants are evaluated between 35-45 days if inoculation for observation of nodules. Thereafter, at step 315, authentication of isolates by observing the inoculated plants with the root nodules, un-inoculated plants remain nodule free. Further biochemical analysis of the root nodules is conducted for testing the efficiency of identified Rhizobium species. [0042] In an exemplary embodiment, the biochemical tests are carried out like Nitrogen estimation, chlorophyll estimation, total protein estimation, estimation of leghaemoglobin for testing the efficiency of the Rhizobium species are explained in detailed. [0043] In another exemplary embodiment, tests for authentication of Rhizobium species are performed. Nitrogen estimation test is carried out by taking 1gram of root nodules extract in digestion tube, is added with 15g of potassium sulphate, 16.7 g of potassium sulphate, 0.01g of copper sulphate, 0.6g of TiO2, 0.3g of Pumice. To the same tube 20ml of sulphuric acid was added and heated the flask at 390oC for 40 minutes to one hour. After heating, cooling and diluting with 250ml of distilled water. Distillate the flask with 75ml of HCl, and add 2-3 drops of methyl red indicator. The collected distillation sample is titrated with 0.1N NaOH until it changes color from red to yellow. Percentage of nitrogen is calculated by using the formula =[(ml standard acid x N of acid) - (ml blank x N of base)] - (ml std base x N of base) x 1.4007 \ Weight of sample in grams. [0044] In another exemplary embodiment, Chlorophyll Estimation test is carried out by taking fresh leaves weighed 500 mg, cleaned and ground in pestle and mortar by adding 10 ml of 80 % acetone. Crushed leaf extract is collected in test tube and centrifuged at 1000 rpm for 15 min. Supernatant is collected and the pellet is re-extracted twice by grinding with 80% acetone. Collected supernatant is estimated for total chlorophyll content by spectrophotometer at 663 nm. [0045] In another exemplary embodiment total protein estimation is carried out in the following way, root nodules are crushed, the sample is collected in test tube and added with 5ml of reagent A (2% Na2CO3, 1% NaK Tartrate and 0.5% CuSO4.5 H2O). Test tube is mixed properly and kept in dark for 10 minutes. After completion of incubation, sample is added with 0.5ml of reagent B (Folin Phenol) and incubated in dark for 30 min, total protein content is estimated in spectrophotometer at 660nm. [0046] In another embodiment, estimation of Leghaemoglobin is carried out. The amount of nitrogen (N) fixed in the symbiotic association between the rhizobia and the plant is closely correlated with the amount of leghaemoglobin (LHb) content of the root nodules of leguminous plants. 50 to 100mg nodules are collected and crushed in 9 volumes of Drabkin's solution in a microfuge tube with a glass rod before centrifugation at 12,000 for 15 minutes. A 0.2m syringe filter is used to filter the supernatant. The filtrate is collected in a micro cuvette, and its absorbance at 540 nm is measured using a spectrophotometer (Kannan, 2015). The above mentioned tests are carried out for identifying efficient Rhizobium species and isolating the species. [0047] Referring to FIG. 4 is a flow chart depicting a method for isolating of host- specific Rhizobium species from Homogenous Soil Mixture (HSM), in accordance with one or more exemplary embodiments of the present disclosure. The method 400 may be carried out in the context of the details of FIG.1.FIG.2. FIG.3. Further, the aforementioned definitions may equally apply to the description below. [0048] The method commences at step 400, isolating Rhizobium by using homogenous soil mixture as source sample. Thereafter, at step, 403 adding 1gm soil to sterile distilled water and subjecting to 10 fold serial dilutions. At step 405, placing the dilutions (10 ^-6 , 10 ^-7 and 10 ^-8 ) on YEMA medium using spread plate method under aseptic conditions at room temperature and observed for 3-6 days until the colonies are developed. Hereafter, observing the obtained Rhizobium for morphological characteristics of the identified species. At step 407, observing the obtained Rhizobium for morphological characteristics of the identified species. Hereafter, at step 409, performing confirmatory tests for microbial identification of the Rhizobium species. [0049] In an exemplary embodiment, Isolation of DNA from rhizobial colonies .The DNA is isolated from the Rhizobial colony. The obtained DNA sample is analysed by colony PCR. DNA is isolated from the bacterial culture; the DNA used in PCR to amplify bacterial 16s rDNA PCR Kit (800). PCR process in the present disclosure uses the rDNA PCR Kit obtained from (TAKARA), Catalog number-RR182A. Using primers from the kit, amplified a 1500bp amplicon and no amplicon was visible in the negative (no DNA) control and the expected sized amplicon (1500bp) was seen in the positive control. The test amplicon of 1500 bp was purified using magnetic beads and the product sequenced by Sanger’s method of DNA sequencing. The sequencing results were assembled and compared with NCBI data base. [0050] In another exemplary embodiment PCR Analysis in polymerase chain reaction 16S rRNA Universal primers are used to amplify the small subunit rRNA of each sample’s culture DNA. The reaction mixture 50 µl contains 4µl bacterial DNA (nearly 200ng), 1µl Taq-DNA polymerase, 5µl of Taq buffer, 5µl of 2mM dNTP mix, 5 µl of forward primer (10 pM/µl) and 5 µl of reverse primer (10 pM/µl). PCR Amplification is carried out in a Bio-Rad thermo cycler run for 30 cycles. Denaturation was done for 94ºC for 20s, annealing at 48ºC for 20s and extension was done at 72ºC for 40s for each cycle, final extension was carried out for 5min at 72ºC at the end of all 30 cycles. The amplified PCR product of approximately 1500 bp is separated on a 1% agarose gel and purified by Qiagen spin columns. [0051] In another exemplary embodiment, 16S rRNA gene sequencing the purified 1542bp PCR product was sequenced using universal primers. The complete 16S rRNA gene sequence of the isolate is subjected to BLAST sequence similarity search and Ez Taxon to identify the nearest taxa. The entire related 16S rRNA gene sequence was downloaded from the database (http://www. nbi .nlm.nih-gov) aligned using the celestial – program. [0052] Referring to FIG.5A and FIG.5B is a schematic representation of preparation of Homogenous Soil Mixture (HSM) in accordance with one or more exemplary embodiments of the present disclosure. [0053] In an exemplary embodiment, 500A is a schematic representation of preparation of Homogenous Soil Mixture(HSM) explains the process of collecting forest soil samples from 9 differently located forests; 3 sites of each forest , about 162 samples from an undisturbed forest area where, KH denotes forest soil of Khammam district, KR denote forest soil of Karimnagar district, ME denotes forest soil of Medak district, MB denotes forest soil of Mahabubnagar district , WG denotes forest soil of Warangal district, AD denotes forest soil of Adilabad district and C as control , n = 3 (experiments were done in triplicates) Homogenous Soil Mixture (soil mixture containing soil samples from KH, KR, ME, MB, WG and AD) [0054] In another embodiment, 500B is a schematic representation of selected sample with efficient rood nodules produced and further used root nodule study for identification, isolation and authentication of Rhizobium species. [0055] Referring to FIG.6 is a schematic representation of method of isolation of Rhizobium species to enhance crop productionin accordance with one or more exemplary embodiments of the present disclosure. [0056] In another exemplary embodiment , 600 is a schematic representation of method of isolation of Rhizobium species to enhance crop production, various soil samples are collected from different forest regions to form a Homogenous Soil Mixture (HSM) and triplicates are maintained with Vigna radiata (green gram), from which the best plants with suitable growth parameters are selected , their root nodules are collected , crushed and sprayed to fresh batch of seeds and these are grown by suitable cultivatable methods, later are selected for isolation of DNA by 16S rRNA sequencing for constructing phylogenetic tree for Rhizobium species for identifying the host- specific efficient Rhizobium species. [0057] Referring to FIG.7 is a schematic representation of phylogenetic tree of Rhizobia present in the Homogenous mixture of all collected Rhizospheresoil. [0058] Referring to FIG.8 is a schematic representation of phylogenetic tree identifying Rhizobium species WG MH290562.1 (Culture deposition number MTCC 12969 &; JCM 33803), in accordance with one or more exemplary embodiments of the present disclosure. [0059] In another exemplary embodiment, 800 is a schematic representation of phylogenetic tree identifying Rhizobium species. WG MH290562.1 (Culture deposition number MTCC 12969 & JCM 33803). [0060] In another exemplary embodiment, the present disclosure studies reveal a highly specific species of Rhizobia that contributes plant growth via nitrogen fixing in Vigna radiata (green gram). Out of 26 species of Rhizobium, WG Strain is found to be efficient in nodule formation and in turn aiding in the enhanced growth and yield of Vigna radiata (green gram). Geographical acclimatization is the reason for physical and compositional analogy of the soil samples. This infers that the growth in the top four plant growth supporting soils is due to the microbial biomass, especially nitrogen fixing bacteria which was proved by means of nitrogen fixing in root nodule by Rhizobium sps. WG. The 16S rRNA gene sequence revealed the Rhizobium as Rhizobium species WG strain [WGMH290562.1 (Culture deposition number MTCC 12969 &; JCM 33803)] and has shown positive result in growth of Vigna radiata (green gram). This species of Rhizobium which is host-specific proved to be the best bio-fertilizer with broad application range. [0061] Although the present disclosure has been described in terms of certain preferred embodiments and illustrations thereof, other embodiments and modifications to preferred embodiments may be possible that are within the principles of the invention. The above descriptions and figures are therefore to be regarded as illustrative and not restrictive. [0062] Thus the scope of the present disclosure is defined by the appended claims and includes both combinations and sub-combinations of the various features described hereinabove as well as variations and modifications thereof, which would occur to persons skilled in the art upon reading the foregoing description.