Title:
ISOLATION AND CHARACTERIZATION OF PLANT REGULATORY SEQUENCES
Document Type and Number:
WIPO Patent Application WO1999028483
Kind Code:
A3
Abstract:
In the search for plant regulatory sequences capable of driving nematode-triggered effector gene expression in feeding structures the promoter tagging appears to be a valuable tool. A large collection of transgenic Arabidopsis thaliana(L.) plants was generated. They were transformed with a beta -glucuronidase gene functioning as a promoter tag. Two T-DNA constructs, pGV1047 and p DELTA gusBin19 were used. Early responses to nematode invasion were of primary interest. Four lines exhibiting GUS activity in syncytia induced by the beet cyst nematode were studied. Reporter gene activation was also identified in galls induced by root knot and ectoparasitic nematodes. Time course studies revealed that the four tags were differentially activated during the development of the feeding structure. T-DNA-flanking regions responsible for the observed responses after nematode infection were isolated and characterized for promoter activity.
Inventors:
BARTHELS NATHALIE (BE)
KARIMI DEHKOR DI MANSOUR (BE)
GHEYSEN GODELIEVE (BE)
KARIMI DEHKOR DI MANSOUR (BE)
GHEYSEN GODELIEVE (BE)
Application Number:
PCT/EP1998/007761
Publication Date:
July 22, 1999
Filing Date:
November 26, 1998
Export Citation:
Assignee:
VLAAMS INTERUNIV INST BIOTECH (BE)
BARTHELS NATHALIE (BE)
KARIMI DEHKOR DI MANSOUR (BE)
GHEYSEN GODELIEVE (BE)
BARTHELS NATHALIE (BE)
KARIMI DEHKOR DI MANSOUR (BE)
GHEYSEN GODELIEVE (BE)
International Classes:
A01H5/00; A01N65/08; C12N5/10; C12N9/22; C12N15/29; C12N15/82; (IPC1-7): C12N15/82; A01H5/00; A01N65/00; C12N5/10; C12N9/22; C12N15/29
Domestic Patent References:
| WO1994010320A1 | 1994-05-11 | |||
| WO1995032288A1 | 1995-11-30 | |||
| WO1993010251A1 | 1993-05-27 |
Other References:
GODDIJN O J M ET AL: "DIFFERENTIAL GENE EXPRESSION IN NEMATODE-INDUCED FEEDING STRUCTURES OF TRANSGENIC PLANTS HARBOURING PROMOTER-GUSA FUSION CONSTRUCTS", PLANT JOURNAL, vol. 4, no. 5, 1 January 1993 (1993-01-01), pages 863 - 873, XP002000605
BARTHELS, N., ET AL.: "REGULATORY SEQUENCES OF ARABIDOPSIS DRIVE REPORTER GENE EXPRESSION IN NEMATODE FEEDING STRUCTURES", THE PLANT CELL, vol. 9, December 1997 (1997-12-01), pages 2119 - 2134, XP002057587
BARTHELS, N., ET AL.: "REGULATORY SEQUENCES OF ARABIDOPSIS DRIVE REPORTER GENE EXPRESSION IN NEMATODE FEEDING STRUCTURES", THE PLANT CELL, vol. 9, December 1997 (1997-12-01), pages 2119 - 2134, XP002057587
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