Title:
ISOLATION OF ENZYMES
Document Type and Number:
WIPO Patent Application WO1997040141
Kind Code:
A3
Abstract:
A method for isolating the DNA encoding an enzyme is presented. The DNA is contained within a support to which the enzyme is linked, wherein the enzyme is further linked to a substrate. When the enzyme reacts with the substrate, the product remains linked to the enzyme. The product linked to the enzyme which is linked to the support containing the DNA encoding the enzyme is isolated, thereby isolating the DNA encoding the enzyme. In this manner an expression library of enzymes produced by mutagenesis can be screened for mutated active enzyme, and thereby obtain the mutated DNA encoding the mutated active enzyme.
Inventors:
NERI DARIO (CH)
DEMARTIS SALVATORE (CH)
HUBER ADRIAN (CH)
VITI FRANCESCA (CH)
TAWFIK DAN S (CH)
WINTER GREGORY PAUL (CH)
DEMARTIS SALVATORE (CH)
HUBER ADRIAN (CH)
VITI FRANCESCA (CH)
TAWFIK DAN S (CH)
WINTER GREGORY PAUL (CH)
Application Number:
PCT/GB1997/001153
Publication Date:
January 08, 1998
Filing Date:
April 25, 1997
Export Citation:
Assignee:
MEDICAL RES COUNCIL (GB)
NERI DARIO (GB)
DEMARTIS SALVATORE (GB)
HUBER ADRIAN (GB)
VITI FRANCESCA (GB)
TAWFIK DAN S (GB)
WINTER GREGORY PAUL (GB)
NERI DARIO (GB)
DEMARTIS SALVATORE (GB)
HUBER ADRIAN (GB)
VITI FRANCESCA (GB)
TAWFIK DAN S (GB)
WINTER GREGORY PAUL (GB)
International Classes:
C07K14/47; C07K14/72; C12N9/00; C12N9/10; C12N15/09; C12N9/12; C12N15/10; C40B40/02; (IPC1-7): C12N9/00; C12N15/00; C12N15/62; C07K14/47
Domestic Patent References:
| WO1995012672A1 | 1995-05-11 | |||
| WO1996017086A1 | 1996-06-06 | |||
| WO1996040723A1 | 1996-12-19 | |||
| WO1997009446A1 | 1997-03-13 |
Other References:
SOUMILLION P ET AL: "SELECTION OF BETA-LACTAMASE ON FILAMENTOUS BACTERIOPHAGE BY CATALYTIC ACTIVITY", JOURNAL OF MOLECULAR BIOLOGY, vol. 237, no. 4, 1994, BALTIMORE US, pages 415 - 422, XP000647071
ILLANGASEKARE M ET AL: "AMINOACYL-RNA SYNTHESIS CATALYZED BY AN RNA", SCIENCE., vol. 267, 3 February 1995 (1995-02-03), WASHINGTON US, pages 643 - 647, XP002044710
LORSCH J R ET AL: "IN VITRO EVOLUTION OF NEW RIBOZYMES WITH POLYNUCLEOTIDE KINASE ACTIVITY", NATURE., vol. 371, 1 September 1994 (1994-09-01), LONDON GB, pages 31 - 36, XP002044711
DATABASE WPI Week 9528, Derwent World Patents Index; AN 95-212960, XP002044713
TAWFIK D S ET AL: "CATELISA: A FACILE GENERAL ROUTE TO CATALYTIC ANTIBODIES", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA., vol. 90, January 1993 (1993-01-01), WASHINGTON US, pages 373 - 377, XP002044712
ILLANGASEKARE M ET AL: "AMINOACYL-RNA SYNTHESIS CATALYZED BY AN RNA", SCIENCE., vol. 267, 3 February 1995 (1995-02-03), WASHINGTON US, pages 643 - 647, XP002044710
LORSCH J R ET AL: "IN VITRO EVOLUTION OF NEW RIBOZYMES WITH POLYNUCLEOTIDE KINASE ACTIVITY", NATURE., vol. 371, 1 September 1994 (1994-09-01), LONDON GB, pages 31 - 36, XP002044711
DATABASE WPI Week 9528, Derwent World Patents Index; AN 95-212960, XP002044713
TAWFIK D S ET AL: "CATELISA: A FACILE GENERAL ROUTE TO CATALYTIC ANTIBODIES", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA., vol. 90, January 1993 (1993-01-01), WASHINGTON US, pages 373 - 377, XP002044712
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