ISOLATION METHOD FOR LOW-ABUNDANCE DIFFERENCE PROTEIN AND APPLICATIONS THEREOF

WIPO Patent Application WO/2017/008380

A1

An isolation method for a low-abundance difference protein, comprising: acquiring a health control group protein sample, a patient control group protein sample, and a to be tested protein sample; immunizing with the healthy control group protein sample as an antigen in preparing a high-abundance protein polyclonal antibody and preparing a high-abundance affinity chromatography column with the high-abundance protein polyclonal antibody as an affinity ligand; flowing the patient control group protein sample through the high-abundance affinity chromatography column and collecting components that passed through; concentrating the components that passes through to serve as an antigen for repeated immunization and preparing a low-abundance protein polyclonal antibody; preparing a low-abundance affinity chromatography column with the low-abundance protein polyclonal antibody as an affinity ligand; flowing the to be tested protein sample through the low-abundance affinity chromatography column and collecting eluted components. The process allows direct isolation in one run to acquire an upregulated protein of an increased content in normal plasma, reduces the complexity of the isolation process, targets a differentially expressed low-abundance protein, and provides increased specificity and targeting.

WO/2007/098085	TESTING LUMENECTOMY SAMPLES FOR MARKERS OF VASCULAR AND NON-VASCULAR DISEASES
WO/2009/035600	ZAP-70 AS PREDICTOR AND MODULATOR OF EFFECTOR FUNCTION OF T CELLS
WO/2015/015043	METHOD FOR PROGNOSIS OF THE EFFICACY OF ORAL IMMUNOTHERAPY FOR THE TREATMENT OF ALLERGY TO PROTEINS IN COW'S MILK

ZHANG GUANJING (CN)
LI RONGXIU (CN)

PCT/CN2015/088825

January 19, 2017

September 02, 2015

Click for automatic bibliography generation  

SHENZHEN HUAKE ANYCHECK INFORMATION TECH LTD (CN)
BIO-TECH ACADEMY(CHINA) CO LTD (CN)

G01N33/68; B01J20/26; C07K1/22; C07K14/435; G01N30/02; G01N30/60; G01N33/531; G01N33/543

CN102460176A	2012-05-16
CN101694485A	2010-04-14
CN101575370A	2009-11-11

KESHISHIAN, H. ET AL.: "Quantitative, Multiplexed Assays for Low Abundance Proteins in Plasma by Targeted Mass Spectrometry and Stable Isotope Dilution", MOLECULAR & CELLULAR PROTEOMICS., vol. 6, no. 12, 15 October 2007 (2007-10-15), pages 2212 - 2229, XP002586395
XIAO, ZHONGHUA; ET AL.: "Analysis of Low Abundance Differential Proteome in Plasma from Hepatocellular Carcinomas Patients", MEDICINE & PUBLIC HEALTH, CHINA MASTER'S THESES FULL-TEXT DATABASE, 15 May 2010 (2010-05-15)

View/Download PDF      PDF Help

Previous Patent: MULTIFUNCTIONAL CONNECTOR AND COMPOSITE CABLE THEREOF

Next Patent: MULTIFUNCTIONAL PILLOW