Title:
KIT FOR DETECTING COLORECTAL CANCER
Document Type and Number:
WIPO Patent Application WO/2017/101756
Kind Code:
A1
Abstract:
A kit for detecting colorectal cancer, which is more particularly a kit for detecting colorectal cancer taking excrement as a detection sample, comprising an Alu detection reagent and PVPP. After repeated explorations it is discovered that Alu, among numerous colorectal cancer markers, can be detected by directly diluting the sample to perform PCR amplification on the premise of proper simple pretreatment, without undergoing the complex steps such as cell disruption and DNA extraction, and good sensitivity and specificity are obtained.
Inventors:
ZOU HONGZHI (CN)
NIU ZHITONG (CN)
NIU ZHITONG (CN)
Application Number:
PCT/CN2016/109627
Publication Date:
June 22, 2017
Filing Date:
December 13, 2016
Export Citation:
Assignee:
CREATIVE BIOSCIENCES (GUANGZHOU) CO LTD (CN)
International Classes:
C12Q1/68
Foreign References:
| CN105349686A | 2016-02-24 | |||
| CN104450680A | 2015-03-25 |
Other References:
YANG SHAOBIN: "Quantifying human long DNA in stool of colorectal cancer patients", MEDICNIE AND PUBLIC HEALTH , CHINA MASTER'S THESES FULL TEXT DATABASE, no. 6, 15 June 2011 (2011-06-15), pages 8
YANG SHAOBIN ET AL.: "Quantifying human long DNA in stool of colorectal cancer patients", CHINESE JOURNAL OF PRACTICAL INTERNAL MEDICINE, vol. 30, no. 9, 30 September 2010 (2010-09-30), pages 835 - 836
GUY, R. A. ET AL.: "Real-Time PCR for Quantification of Giardia and Cryptosporidium in Environmental Water Samples and Sewage", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 69, no. 9, 30 September 2003 (2003-09-30), pages 5183, XP008104236, DOI: doi:10.1128/AEM.69.9.5178-5185.2003
BOYNTON, K. A.: "DNA Integrity as a Potential Marker for Stool-based Detection of Colorectal Cancer", CLINICAL CHEMISTRY, vol. 49, no. 7, 31 December 2003 (2003-12-31), pages 1058 - 1065, XP002345190, DOI: doi:10.1373/49.7.1058
WU YONG ET AL.: "Significance of molecule markers and fecal occult blood test in the diagnosis of colorectal cancer", CHINESE JOURNAL OF LABORATORY MEDICINE, vol. 30, no. 12, pages 1340 - 1343
YANG SHAOBIN ET AL.: "Quantifying human long DNA in stool of colorectal cancer patients", CHINESE JOURNAL OF PRACTICAL INTERNAL MEDICINE, vol. 30, no. 9, 30 September 2010 (2010-09-30), pages 835 - 836
GUY, R. A. ET AL.: "Real-Time PCR for Quantification of Giardia and Cryptosporidium in Environmental Water Samples and Sewage", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 69, no. 9, 30 September 2003 (2003-09-30), pages 5183, XP008104236, DOI: doi:10.1128/AEM.69.9.5178-5185.2003
BOYNTON, K. A.: "DNA Integrity as a Potential Marker for Stool-based Detection of Colorectal Cancer", CLINICAL CHEMISTRY, vol. 49, no. 7, 31 December 2003 (2003-12-31), pages 1058 - 1065, XP002345190, DOI: doi:10.1373/49.7.1058
WU YONG ET AL.: "Significance of molecule markers and fecal occult blood test in the diagnosis of colorectal cancer", CHINESE JOURNAL OF LABORATORY MEDICINE, vol. 30, no. 12, pages 1340 - 1343
Attorney, Agent or Firm:
UNITALEN ATTORNEYS AT LAW (CN)
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