Title:
KIT FOR DETECTING HIGHLY PATHOGENIC AVIAN INFLUENZA VIRUS SUBTYPE H5N1
Document Type and Number:
WIPO Patent Application WO/2010/103583
Kind Code:
A1
Abstract:
Disclosed are an immunoassay kit and an immunoassay method for detecting highly pathogenic avian influenza virus subtype H5N1 rapidly, conveniently and specifically. Also disclosed are an immunochromatographic detection kit and an immunochromatographic detection method for detecting the virus subtype H5N1 rapidly, conveniently and specifically. It is found that a monoclonal antibody 4G6 produced by using the virus subtype H5N1 as an immunogen does not react with the a subtype H5N2 virus or a subtype H5N3 virus and reacts only with a subtype H5N1 virus specifically. It is also found that only an avian influenza virus subtype H5N1 can be detected specifically by an immunoassay utilizing the monoclonal antibody 4G6. It is further found that the sensitivity of the detection of immunochromatography can be increased by adding a nonionic surfactant and a water-soluble vinyl polymer having a polar group containing an oxygen atom and a nitrogen atom to a developing solution to be used in the immunochromatography.
Inventors:
NAKAYA TAKAAKI (JP)
DU ANARIWA (JP)
SHIBAI YUSUKE (JP)
ITOH DAISUKE (JP)
IWAMOTO HISAHIKO (JP)
DU ANARIWA (JP)
SHIBAI YUSUKE (JP)
ITOH DAISUKE (JP)
IWAMOTO HISAHIKO (JP)
Application Number:
PCT/JP2009/005065
Publication Date:
September 16, 2010
Filing Date:
October 01, 2009
Export Citation:
Assignee:
UNIV OSAKA (JP)
TANAKA PRECIOUS METAL IND (JP)
NAKAYA TAKAAKI (JP)
DU ANARIWA (JP)
SHIBAI YUSUKE (JP)
ITOH DAISUKE (JP)
IWAMOTO HISAHIKO (JP)
TANAKA PRECIOUS METAL IND (JP)
NAKAYA TAKAAKI (JP)
DU ANARIWA (JP)
SHIBAI YUSUKE (JP)
ITOH DAISUKE (JP)
IWAMOTO HISAHIKO (JP)
International Classes:
G01N33/569; G01N33/543; G01N33/577
Foreign References:
| JP2008104450A | 2008-05-08 | |||
| JP2007322310A | 2007-12-13 | |||
| JP2005291780A | 2005-10-20 |
Other References:
DU A ET AL.: "Detection of circulating Asian H5N1 viruses by a newly established monoclonal antibody.", BIOCHEM BIOPHYS RES COMMUN., vol. 378, no. 2, 9 January 2009 (2009-01-09), pages 197 - 202, XP025745429
Attorney, Agent or Firm:
SAEKI, NORIO (JP)
Norio Saeki (JP)
Norio Saeki (JP)
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