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Title:
KIT FOR ISOLATING CIRCULATING TUMOR CELLS, CONTAINER FOR ISOLATING CIRCULATING TUMOR CELLS, AND METHOD FOR ISOLATING CIRCULATING TUMOR CELLS
Document Type and Number:
WIPO Patent Application WO/2023/181468
Kind Code:
A1
Abstract:
Provided is a kit for isolating circulating tumor cells, the kit being capable of increasing the recovery rate of circulating tumor cells, not only when a specimen, which has been stored for not a long period of time after collection, is used, but also when a specimen, which has been stored for several days after the collection of blood, is used. The kit for isolating circulating tumor cells according to the present invention is used for isolating circulating tumor cells in the blood, and comprises a blood collection container in which an aqueous solution is contained and a predetermined amount of blood is collected, and a cell isolation container in which a cell isolation material having a specific gravity of 1.065-1.080 at 25 °C is contained, wherein: the aqueous solution contains an anticoagulant, and a low-molecular compound having a molecular weight of 75-500 or a high-molecular compound having a number-average molecular weight of 2,000-200,000 (exclusive of 200,000); and when a physiological saline, of an equal amount as a predetermined amount of the blood collected in the blood collection container, is collected into the blood collection container and a mixed solution, in which the physiological saline and the aqueous solution are mixed, is obtained, the osmotic pressure of the mixed solution is 270-350 mOsm/L.

Inventors:
UCHIYAMA TAKAYA (JP)
KANDA MARIKA (JP)
KOMAI KUNIYA (JP)
INOUE TOMONORI (JP)
Application Number:
PCT/JP2022/038554
Publication Date:
September 28, 2023
Filing Date:
October 17, 2022
Export Citation:
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Assignee:
SEKISUI MEDICAL CO LTD (JP)
International Classes:
G01N33/48; C12M1/00; C12N1/02; C12N5/09
Domestic Patent References:
WO2015046557A12015-04-02
WO2021182575A12021-09-16
WO2021112119A12021-06-10
WO2013168767A12013-11-14
Foreign References:
JP2021500585A2021-01-07
JP2019211216A2019-12-12
JP2005501236A2005-01-13
JP2020523577A2020-08-06
JP2014524027A2014-09-18
JP2015506482A2015-03-02
JP2007271388A2007-10-18
JPH01199158A1989-08-10
Attorney, Agent or Firm:
OSAKA FRONT (JP)
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