Title:
LABEL-FREE BIOCHEMICAL REACTION DETECTION METHOD
Document Type and Number:
WIPO Patent Application WO/2021/052146
Kind Code:
A1
Abstract:
A label-free biochemical reaction detection method, comprising: S100, filling a pipe (4) with an electrolyte solution; S200, selecting receptor molecule A (6) capable of reacting with target molecule B (7) and modifying receptor molecule A (6) on the inner wall of the pipe; S300, introducing a dispersed phase into the pipe (4) for same to form a dispersed phase area (3), and retaining the dispersed phase area (3) in the pipe (4); S400, measuring and recording a contact angle of the dispersed phase area (3) in the pipe (4) and the total resistance of the solution in the pipe (4) before reacting with the target molecule (7); S500, introducing a solution or fluid possibly containing target molecule B (7) into the pipe (4) and reacting with receptor molecule A (6); S600, repeating step S300 to step S400, comparing changes in the contact angle and resistance of the dispersed phase before and after the reaction; if the change is great, then the solution or fluid contains target molecule B (7); and if not, same does not contain target molecule B (7). This serves as an inexpensive, highly sensitive, quick, and label-obviating label-free detection method for a biochemical molecular reaction.
Inventors:
XIE YANBO (CN)
MA YU (CN)
SUN MIAO (CN)
MA YU (CN)
SUN MIAO (CN)
Application Number:
PCT/CN2020/112228
Publication Date:
March 25, 2021
Filing Date:
August 28, 2020
Export Citation:
Assignee:
UNIV NORTHWESTERN POLYTECHNICAL (CN)
International Classes:
G01N33/53; G01N13/02
Foreign References:
| CN110702563A | 2020-01-17 | |||
| US20120142017A1 | 2012-06-07 | |||
| CN1415965A | 2003-05-07 | |||
| CN101561444A | 2009-10-21 | |||
| CN108645905A | 2018-10-12 | |||
| CN109540984A | 2019-03-29 | |||
| CN109453827A | 2019-03-12 |
Attorney, Agent or Firm:
XI'AN TONG DA PATENT AGENCY CO., LTD. (CN)
Download PDF:
Previous Patent: SECURITY VERIFICATION METHOD AND SYSTEM, COMPUTER DEVICE AND MEDIUM
Next Patent: DATA TRANSMISSION METHOD AND RELATED DEVICES
Next Patent: DATA TRANSMISSION METHOD AND RELATED DEVICES