Title:
LATERAL FLOW CHROMATOGRAPHY TEST PAPER BASED ON MERCAPTOPHENYLBORONIC ACID FUNCTIONALIZED GOLD NANOPARTICLES, AND DETECTION METHOD THEREFOR
Document Type and Number:
WIPO Patent Application WO/2023/087480
Kind Code:
A1
Abstract:
A lateral flow chromatography test paper based on mercaptophenylboronic acid functionalized gold nanoparticles, and a detection method. The lateral flow chromatography test paper comprises a flat plate (1), a sample pad (2), a nitrocellulose membrane (3) and an absorption pad (4). The lateral flow chromatography detection method can be completed by means of the steps: preparing mercaptophenylboronic acid functionalized gold nanoparticles, preparing a gold-mercaptophenylboronic acid-bacteria conjugate, preparing a lateral flow chromatography test paper strip, and performing lateral flow chromatography detection. Using the strong binding power of phenylboric acid and bacteria overcomes the limitations of a double-antibody sandwich method in conventional lateral flow immunoassays, greatly reducing detection costs, and improving a detection limit; qualitative and quantitative results of pathogenic microorganisms can be obtained by means of the present method, specificity is strong, sensitivity is high, the detection process is convenient and fast, and the detection result is safe and reliable.
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Inventors:
JU YANMIN (CN)
DAI JIANJUN (CN)
WU PENGCHENG (CN)
DAI JIANJUN (CN)
WU PENGCHENG (CN)
Application Number:
PCT/CN2021/140031
Publication Date:
May 25, 2023
Filing Date:
December 21, 2021
Export Citation:
Assignee:
UNIV CHINA PHARMA (CN)
International Classes:
G01N33/569; A61K31/69
Domestic Patent References:
| WO2021218661A1 | 2021-11-04 |
Foreign References:
| CN109444400A | 2019-03-08 | |||
| CN110393725A | 2019-11-01 | |||
| CN106568951A | 2017-04-19 | |||
| CN113447647A | 2021-09-28 | |||
| CN110292652A | 2019-10-01 |
Attorney, Agent or Firm:
NANJING SUGAO PATENT AND TRADEMARK FIRM ( ORDINARY PARTNERSHIP ) (CN)
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