BACKGROUND OF THE INVENTION Laundry and cleaning composition having antimicrobial activities are of interest to consumers. Efforts to formulate antimicrobial hand soaps and cleaning compositions are well known. Efforts to produce laundry compositions comprising enzymes having microbial properties are also known, for example, U.S. 5,356,803, issued October 18, 1994 to Carpenter et al.

In spite of such efforts, there continues to be a need for laundry and cleaning compositions having antimicrobial activity. An object of the invention is to provide laundry and cleaning compositions having antimicrobial activity containing hexosaminidase enzymes. These and other objects will be apparent from the detailed description herein.

BACKGROUND ART US 5,356,803 is directed to the use of Type II endoglycosidases (Endo-D, Endo-H, Endo-F and PNGaseF) in laundry and cleaning compositions. See also: US 5,258,304; US 5,395,541; J. Biol. Chem. (1996), 271 (52), 33425-33432; WO 96/25424; Nat. Struct. Biol. (1996), 3(7), 638-648; Microbiology (1994), 140 (12), 3399-3406; J. Bacteriol. (1994), 176(9), 2640-7; Proc. Nat'l Acad. Sci. USA (1993), 90(14), 6751-5; Proc. Natl. Acad. Sci. USA (1985), 82 (23), 7830-4; and WO 96/36700.

SUMMARY OF THE INVENTION The present invention relates to laundry or cleaning products comprising one or more hexosaminidase enzymes, preferably at a level of from about 0.001% to about 1%, more preferably from about 0.01% to about 0.5%, by weight ofthe composition. More preferred are hexosaminidases having minimum inhibitory concentration ("MIC") for antimicrobial activity of less than about 0.125%, most preferably less than about 0.025%, and/or the ability to remove biofilm. The present invention also relates to a method for laundering fabrics (preferably clothes), said method comprising contacting fabrics in need of cleaning with an aqueous solution containing an effective amount of one or more hexosaminidase enzymes, preferably an aqueous solution of a composition according to the present invention. The present invention further relates to a method for cleaning hard surfaces, such as dishes and tableware, said method comprising contacting the hard surface in need of cleaning with an aqueous solution containing an effective amount of one or more hexosaminidase enzymes, preferably an aqueous solution of a composition according to the present invention, and more preferably for dishes and tableware in an automatic di shwashing machine.

As used herein, the term "hexosaminidase enzyme" means those enzymes whose activity is for the hydrolysis of terminal non-reducing N-acetyl-D- hexosamine residues in N-acetyl- -D-hexosaminides, thereby acting on N- acetylglucosides and N-acetylgalactosides, and are classified under the class of enzymes EC 3.2.1.52 (also known as "-N-acetylhexosamim.dase"). N-Acetyl- -D- hexosaminidase is also referred to as "chitobiosidases" or "exochitinase" (see for example, WO 96/36700). Hexosaminidases are known, for example those enzymes having the amino acid SEQ. ID No. 1-5 and 10-11 are classified in the literature as hexosaminidases. Furthermore, DNA sequences encoding for hexosaminidases are known, for example those having the SEQ ID No. 6-9. Examples of such disclosures in the literature include: J. Biol. Chem. (1996), 271 (52), 33425-33432; WO 96/25424; Nat. Struct. Biol. (1996), 3(7), 638-648; Microbiology (1994), 140 (12), 3399-3406; J. Bacteriol. (1994), 176(9), 2640-7; Proc. Nat'l Acad. Sci. USA (1993), 90(14), 6751-5; Proc. Natl. Acad. Sci. USA (1985), 82 (23), 7830-4; and WO 96/36700. In addition, a commercially available hexosaminidase is "exo-P-N- acetylglucosaminidase" sold by Boehringer. Specific N-acetyl- -D- hexosaminidases from Saccharomyces cerevisiae DSM No. 9944 or DSM 9945 are also described in WO 96/36700.

Thus, more specifically, the invention encompasses laundry and cleaning compositions comprising a hexosaminidase enzyme exhibiting antimicrobial activity, which enzyme: i) is encoded by a DNA sequence comprising or included in at least one of the sequences of SEQ ID Nos 6-9, or a sequence homologous thereto encoding a hexosaminidase polypeptide, ii) is immunologically reactive with an antibody raised against a highly purified hexosaminidase encoded by the DNA sequence defined in i), and is specific for hexosaminidase, iii) is immunologically reactive with an antibody raised against a highly purified hexosaminidase having SEQ ID Nos 1-5, 10 or 11, and is specific for hexosaminidase, or iv) is a hexosaminidase having SEQ ID Nos 1-5, 10 or 11, or a hexosaminidase polypeptide sequence homologous thereto.

The terms "homologue" and "homologous" as used herein indicate a polypeptide encoded by DNA which hybridizes to the same probe as the DNA coding for an hexosaminidase enzyme under certain specified conditions (such as presoaking in 5xSSC and prehybridizing for 1 h at -400C in a solution of 5xSSC, SxDenhardt's solution, and 50 pg of denatured sonicated calf thymus DNA, followed by hybridization in the same solution supplemented with 50 pCi 32-P-dCTP labelled probe for 18 h at -400C and washing three times in 2xSSC, 0.2% SDS at 400C for 30 minutes). More specifically, the term is intended to refer to a DNA sequence which is at least 70% homologous to any of SEQ ID Nos 6-9, or the DNA encoding for the hexosaminidases of SEQ ID Nos 1-5, 10 or 11 including at least 75%, at least 80%, at least 85%, at least 90% or even at least 95% with any of these sequences. The term is intended to include modifications of any of such DNA sequences, such as nucleotide substitutions which do not give rise to another amino acid sequence of the polypeptide encoded by the sequence, but which correspond to the codon usage of the host organism into which a DNA construct comprising any of the DNA sequences is introduced or nucleotide substitutions which do give rise to a different amino acid sequence and therefore, possibly, a different amino acid sequence and therefore, possibly, a different protein structure which might give rise to a hexosaminidase mutant with different properties than the native enzyme. Other examples of possible modifications are insertion of one or more nucleotides into the sequence, addition of one or more nucleotides at either end of the sequence, or deletion of one or more nucleotides at either end or within the sequence.

The term "biofiim" as used herein means irreversibly bound bacteria to a surface.

All parts, percentages and ratios used herein are expressed as percent weight unless otherwise specified. All documents cited are, in relevant part, incorporated herein by reference.

DETAILED DESCRIPTION OF THE INVENTION Hexosaminidases: Hexosaminidases have been identified herein as particularly useful for their cleaning and/antimicrobial properties in laundry and cleaning compositions.

A hexosaminidase enzyme useful in the present invention may be isolated by a general method involving: - cloning, in suitable vectors, a DNA library from a selected species, - transforming suitable host cells with said vectors, - culturing the host cells under suitable conditions to express any enzyme of interest encoded by a clone in the DNA library, and - screening for positive clones by determining any hexosaminidase activity of the enzyme produced by such clones.

The DNA sequence encoding for the desired hexosaminidase enzyme may subsequently be inserted into a recombinant expression vector. This may be any vector which may conveniently be subjected to recombinant DNA procedures, and the choice of vector will often depend on the host cell into which it is to be introduced. Thus, the vector may be an autonomously replicating vector, i.e. a vector which exists as an extrachromosomal entity, the replication of which is independent of chromosomal replication, e.g. a plasmid. Alternatively, the vector may be one which, when introduced into a host cell, is integrated into the host cell genome and replicated together with the chromosome(s) into which it has been integrated.

In the vector, the DNA sequence encoding the hexosaminidase should be operably connected to a suitable promoter and terminator sequence. The promoter may be any DNA sequence which shows transcriptional activity in the host cell of choice and may be derived from genes encoding proteins either homologous or heterologous to the host cell. The procedures used to ligate the DNA sequences coding for the hexosaminidase, the promoter and the terminator, respectively, and to insert them into suitable vectors are well known to persons skilled in the art (cf., for instance, Sambrook et al., Molecular Cloning. A Laboratorv Manual, Cold Spring Harbor, NY 1989).

The host cell which is transformed with the DNA sequence encoding the enzyme useful for the present invention compositions is preferably a eukaryotic cell, in particular a fungal cell such as a yeast or filamentous fungal cell. Fungal cells may be transformed by a process involving protoplast formation and transformation of the protoplasts followed by regeneration of the cell wall in a manner known in the art. The host cell may also be a yeast cell, e.g. a strain of Saccharomyces, in particular Saccharomyces cerevisiae.

The medium used to culture the transformed host cells may be any conventional medium suitable for growing the host cells in question. The expressed hexosaminidase may conveniently be secreted into the culture medium and may be recovered therefrom by well-known procedures including separating the cells from the medium by centrifugation or filtration, precipitating proteinaceous components of the medium by means of a salt such as ammonium sulphate, followed by chromatographic procedures such as ion exchange chromatography, affinity chromatography, or the like.

The thus purified hexosaminidase may be employed for immunization of animals for the production of antibodies. More specifically, antiserum against the hexosaminidase may be raised by immunizing rabbits (or other rodents) according to the procedure described by N. Axelsen et al. in: A Manual of Ouantitative Immunoelectrophoresis. Blackwell Scientific Publications, 1973, Chapter 23, or A.

Johnstone and R. Thorpe, Immunochemistrv in Practice, Blackwell Scientific Publications, 1982 (more specifically pp. 27-31). Purified immunoglobulins may be obtained from the antisera, for example by salt precipitation ((NH4) 2S04), followed by dialysis and ion exchange chromatography, e.g. on DEAE-Sephadex.

Immunochemical characterization of proteins may be done either by Outcherlony double-diffusion analysis (0. Ouchterlony in: Handbook of Experimental Immunology (D.M. Weir, Ed.), Blackwell Scientific Publications, 1967, pp. 655- 706), by crossed immunoelectrophoresis (N. Axelsen et al supra, Chapters 3 and 4), or by rocket immunoelectrophoresis (N. Axelsen et al., Chapter 2).

The enzyme preparation useful in the present invention compositions may be prepared in accordance with methods known in the art and may be in the form of a liquid or a dry preparation. For instance, the enzyme preparation may be in the form of a granulate or a microgranulate. The enzyme to be included in the preparation may also be stabilized in accordance with methods known in the art.

The enzyme preparation useful in the present compositions may, in addition to a hexosaminidase, contain one or more other detergent enzymes and/or other plant cell wall degrading enzymes, for instance those with cellulytic, xylanolytic or pectinolytic activities such as xylanase, arabinanase, rhamnogalacturonase, pectin acetylesterase, galactanase, polygalacturonase, pectin lyase, pectate lyase, endo- glucanase or pectin methylesterase. The additional enzymes may be producible by means of a microorganism belonging to the genus Aspergillus, preferably aspergillus niger, Aspergillus aculeatus, Aspergillus awamoi or Aspergillus oryzae.

Test Methods: The potency of antimicrobial activity of the hexosaminidase useful herein is measured by determining the minimum inhibitory concentration (MIC) of enzyme required to inhibit growth of bacteria/fungi. For example, the bacteria used can include Escherichia coli 25922, 11229, Staphylococcus aureaus 25932,6538, Psudomonas aeruginosa 27853 and Proteus mirabilis 12453.

The minimum inhibitory concentration of enzyme to inhibit growth of bacteria is determined in Robbins Scientific 96 well microassay Microplates with 50 , ul wells. 105 ul of stock solutions of the single bacteria (from ATCC) are diluted in 15 ml of growth medium based on Tryptic Soy Broth/Agar (Carr-Scarrborough).

The enzyme samples are diluted to 8000 ppm active enzyme in buffer solution. 10 p l of buffer is added to each well. 10 pl of enzyme solution is added in the first well.

The enzyme solution is diluted in subsequent wells by 50%, by sequential transfer of 10 pl. After final dilution 10 pl of bacteria with growth medium is added to each well. All manipulations are performed with sterile material. All plates are incubated at 37"C for 12-24 hours. The growth of bacteria is assessed under a microscope.

The minimum inhibitory concentration is determined by the lowest enzyme concentration which does not show bacteria growth. Preferred hexosaminidases for use herein have antimicrobial activity of less than about 0.125%.

Scanning electron microscopy can be used to determine biofilm removal.

Preferred hexosaminidases for use herein have the ability to remove biofilm.

Cleaning Composition Ingredients and Detergent Compositions The detergent compositions of the invention contain laundry or cleaning composition ingredients as described hereinafter. The precise nature of these components, and levels of incorporation thereof will depend on the physical form of the composition, and the nature of the cleaning operation for which it is to be used.

The detergent compositions according to the invention can be liquid, paste, gels, bars, tablets, powder or granular forms. Granular compositions can also be in "compact" form, the liquid compositions can also be in a "concentrated" form.

The compositions of the invention may for example, be formulated as hand and machine laundry detergent compositions including laundry additive compositions and compositions suitable for use in the soaking and/or pretreatment of stained fabrics, rinse added fabric softener compositions. Pre-or post treatment of fabric include gel, spray and liquid fabric conditioning compositions.

When formulated as compositions suitable for use in a laundry machine washing method, the compositions of the invention preferably contain both a surfactant and a builder compound and additionally one or more detergent components preferably selected from organic polymeric compounds, bleaching agents, additional enzymes, suds suppressors, dispersants, lime-soap dispersants, soil suspension and anti-redeposition agents and corrosion inhibitors. Laundry compositions can also contain softening agents, as additional detergent components.

The compositions of the invention can also be used as detergent additive products. Such additive products are intended to supplement or boost the performance of conventional detergent compositions.

If needed the density of the laundry detergent compositions herein ranges from 400 to 1200 g/litre, preferably 600 to 950 g/litre of composition measured at 20"C.

The "compact" form of the compositions herein is best reflected by density and, in terms of composition, by the amount of inorganic filler salt; inorganic filler salts are conventional ingredients of detergent compositions in powder form; in conventional detergent compositions, the filler salts are present in substantial amounts, typically 17-35% by weight of the total composition.

In the compact compositions, the filler salt is present in amounts not exceeding 15% of the total composition, preferably not exceeding 10%, most preferably not exceeding 5% by weight of the composition.

The inorganic filler salts, such as meant in the present compositions are selected from the alkali and alkaline-earth-metal salts of sulphates and chlorides. A preferred filler salt is sodium sulphate.

Liquid detergent compositions according to the present invention can also be in a "concentrated form", in such case, the liquid detergent compositions according the present invention will contain a lower amount of water, compared to conventional liquid detergents.

Typically the water content of the concentrated liquid detergent is preferably less than 40%, more preferably less than 30%, most preferably less than 20% by weight of the detergent composition.

Surfactants Preferably, the detergent compositions according to the present invention comprise a surfactant or surfactant system wherein the surfactant can be selected from nonionic and/or anionic and/or cationic and/or ampholytic and/or zwitterionic and/or semi-polar nonionic surfactants.

The surfactant is typically present at a level of from 0.1% to 60% by weight.

More preferred levels of incorporation are 1% to 35% by weight, most preferably from 1% to 30% by weight of detergent compositions in accord with the invention.

The surfactant is preferably formulated to be compatible with enzyme components present in the composition. In liquid or gel compositions the surfactant is most preferably formulated such that it promotes, or at least does not degrade, the stability of any enzyme in these compositions.

Examples of suitable nonionic, anionic, cationic, ampholytic, zwitterionic and semi-polar nonionic surfactants are disclosed in U.S. Patent Nos. 5,707,950 and 5,576,282.

Highly preferred nonionic surfactants are polyhydroxy fatty acid amide surfactants of the formula: R2 -C(O)-N(R1)-Z, wherein R1 is H, or R1 is C 1-4 hydrocarbyl, 2-hydroxy ethyl, 2-hydroxy propyl or a mixture thereof, R2 is Cos 31 hydrocarbyl, and Z is a polyhydroxyhydrocarbyl having a linear hydrocarbyl chain with at least 3 hydroxyls directly connected to the chain, or an alkoxylated derivative thereof. Preferably, R1 is methyl, R2 is a straight C1l l5 alkyl or C 16-18 alkyl or alkenyl chain such as coconut alkyl or mixtures thereof, and Z is derived from a reducing sugar such as glucose, fructose, maltose, lactose, in a reductive amination reaction.

Highly preferred anionic surfactants include alkyl alkoxylated sulfate surfactants hereof are water soluble salts or acids of the formula RO(A)mSO3M wherein R is an unsubstituted C10-C24 alkyl or hydroxyalkyl group having a C10- C24 alkyl component, preferably a C12-C20 alkyl or hydroxyalkyl, more preferably C1 2-C18 alkyl or hydroxyalkyl, A is an ethoxy or propoxy unit, m is greater than zero, typically between about 0.5 and about 6, more preferably between about 0.5 and about 3, and M is H or a cation which can be, for example, a metal cation (e.g., sodium, potassium, lithium, calcium, magnesium, etc.), ammonium or substituted- ammonium cation. Alkyl ethoxylated sulfates as well as alkyl propoxylated sulfates are contemplated herein.

When included therein, the laundry detergent compositions of the present invention typically comprise from about 1% to about 40%, preferably from about 3% to about 20% by weight of such anionic surfactants.

Highly preferred cationic surfactants are the water-soluble quaternary ammonium compounds useful in the present composition having the formula: R1 R2R3R4N+X- wherein R1 is C8-C16 alkyl, each of R2, R3 and R4 is independently C1-C4 alkyl, C1-C4 hydroxy alkyl, benzyl, and (C2H40)xH where x has a value from 2 to 5, and X is an anion. Not more than one oft2, R3 or R4 should be benzyl.

When included therein, the detergent compositions of the present invention typically comprise from 0.2% to about 25%, preferably from about 1% to about 8% by weight of such cationic surfactants.

When included therein, the detergent compositions of the present invention typically comprise from 0.2% to about 15%, preferably from about 1% to about 10% by weight of such ampholytic surfactants.

When included therein, the detergent compositions of the present invention typically comprise from 0.2% to about 15%, preferably from about 1% to about 10% by weight of such zwitterionic surfactants.

When included therein, the detergent compositions of the present invention typically comprise from 0.2% to about 15%, preferably from about 1% to about 10% by weight of such semi-polar nonionic surfactants.

The detergent composition of the present invention may further comprise a cosurfactant selected from the group of primary or tertiary amines.

Suitable primary amines for use herein include amines according to the formula R1NH2 wherein R1 is a C6-C12, preferably C6-C10 alkyl chain or R4X(CH2)n, X is -O-, -C(O)NH- or -NH-, R4 is a C6-C12 alkyl chain n is between 1 to 5, preferably 3. R1 alkyl chains may be straight or branched and may be interrupted with up to 12, preferably less than 5 ethylene oxide moieties.

Preferred amines according to the formula herein above are n-alkyl amines.

Suitable amines for use herein may be selected from 1-hexylamine, 1 -octylamine, 1- decylamine and laurylamine. Other preferred primary amines include C8-C10 oxypropylamine, octyloxypropylamine, 2-ethylhexyl-oxypropylamine, lauryl amido propylamine and amido propylamine.

Suitable tertiary amines for use herein include tertiary amines having the formula R1R2R3N wherein R1 and R2 are C1-Cg alkylchains or R3 is either a C6-C12, preferably C6-C10 alkyl chain, or R3 is R4X(CH2)n, whereby X is -O-, -C(O)NH- or -NH-, R4 is a C4-C12, n is between 1 to 5, preferably 2-3. K5 is II or C1-C2 alkyl and x is between 1 to 6.

R3 and R4 may be linear or branched ; R3 alkyl chains may be interrupted with up to 12, preferably less than 5, ethylene oxide moieties.

Preferred tertiary amines are R1R2R3N where RI is a C6-C12 alkyl chain, R2 and R3 are C1-C3 alkyl or where R5 is H or CH3 and x = 1-2.

Also preferred are the amidoamines of the formula: wherein R1 is C6-C12 alkyl; n is 2-4, preferably n is 3; R2 and R3 is C1-C4 Most preferred amines of the present invention include 1 -octylamine, 1- hexylamine, 1-decylamine,. l-dodecylamine,C8-l Ooxypropylamine, N coco I- 3 diaminopropane, coconutalkyldimethylamine, lauryldimethylamine, lauryl bis(hydroxyethyl)amine, coco bis(hydroxyehtyl)amine, lauryl amine 2 moles propoxylated, octyl amine 2 moles propoxylated, lauryl amidopropyldimethylamine, C8-10 amidopropyldimethylamine and C10 amidopropyldimethylamine.

The most preferred amines for use in the compositions herein are 1- hexylamine, 1-octylamine, 1-decylamine, 1-dodecylamine. Especially desirable are n-dodecyldimethylamine and bishydroxyethylcoconutalkylamine and oleylamine 7 times ethoxylated, lauryl amido propylamine and cocoamido propylamine.

The surfactant and surfactant system of the present invention is preferably formulated to be compatible with enzyme components present in the composition.

In liquid or gel compositions the surfactant is most preferably formulated such that it promotes, or at least does not degrade, the stability of any enzyme in these compositions.

Builders The compositions according to the present invention may further comprise a builder or builder system. Any conventional builder system is suitable for use herein including aluminosilicate materials, silicates, polycarboxylates, alkyl- or alkenyl- succinic acid and fatty acids, materials such as ethylenediamine tetraacetate, diethylene triamine pentamethyleneacetate, metal ion sequestrants such as aminopolyphosphonates, particularly ethylenediamine tetramethylene phosphonic acid and diethylene triamine pentamethylenephosphonic acid. Phosphate builders can also be used herein.

The present invention may include a suitable builder or detergency salt. The level of detergent salt/builder can vary widely depending upon the end use of the composition and its desired physical form. When present, the compositions will typically comprise at least about 1% builder and more typically from about 10% to about 80%, even more typically from about 15% to about 50% by weight, of the builder. Lower or higher levels, however, are not meant to be excluded.

Inorganic or P-containing detergent salts include, but are not limited to, the alkali metal, ammonium and alkanolammonium salts of polyphosphates (exemplified by the tripolyphosphates, pyrophosphates, and glassy polymeric meta- phosphates), phosphonates, phytic acid, silicates, carbonates (including bicarbonates and sesquicarbonates), sulphates, and aluminosilicates. However, non-phosphate salts are required in some locales. Importantly, the compositions herein function surprisingly well even in the presence of the so-called "weak" builders (as compared with phosphates) such as citrate, or in the so-called "underbuilt" situation that may occur with zeolite or layered silicate builders.

Organic detergent builders suitable for the purposes of the present invention include, but are not restricted to, a wide variety of polycarboxylate compounds. As used herein, "polycarboxylate" refers to compounds having a plurality of carboxylate groups, preferably at least 3 carboxylates. Polycarboxylate builder can generally be added to the composition in acid form, but can also be added in the form of a neutralized salt. When utilized in salt form, alkali metals, such as sodium, potassium, and lithium, or alkanolammonium salts are preferred.

Examples of suitable silicate builders, carbonate salts, aluminosilicate <BR> <BR> <BR> <BR> builders, polycarboxylate builders, citrate builders, 3,3 -dicarboxy-4-oxa-l 6- hexanedioate builders and related compounds disclosed in U.S. Patent No.

4,566,984, to Bush, succinic acid builders, phosphorous-based builders and fatty acids, are disclosed in U.S. Patent Nos. 5,576,282, 5,728,671 and 5,707,950.

Additional suitable builders can be an inorganic ion exchange material, commonly an inorganic hydrated aluminosilicate material, more particularly a hydrated synthetic zeolite such as hydrated zeolite A, X, B, HS or MAP.

Specific polycarboxylates suitable for the present invention are polycarboxylates containing one carboxy group include lactic acid, glycolic acid and ether derivatives thereof as disclosed in Belgian Patent Nos. 831,368, 821,369 and 821,370. Polycarboxylates containing two carboxy groups include the water-soluble salts of succinic acid, malonic acid, (ethylenedioxy) diacetic acid, maleic acid, diglycollic acid, tartaric acid, tartronic acid and fumaric acid, as well as the ether carboxylates described in German Offenlegenschrift 2,446,686, and 2,446,687 and U.S. Patent No. 3,935,257 and the sulfinyl carboxylates described in Belgian Patent No. 840,623. Polycarboxylates containing three carboxy groups include, in particular, water-soluble citrates, aconitrates and citraconates as well as succinate derivatives such as the carboxymethyloxysuccinates described in British Patent No.

1,379,241, lactoxysuccinates described in Netherlands Application 7205873, and the oxypolycarboxylate materials such as 2-oxa-1,1,3-propane tricarboxylates described in British Patent No. 1,387,447.

Polycarboxylates containing four carboxy groups include oxydisuccinates disclosed in British Patent No. 1,261,829, 1,1,2,2-ethane tetracarboxylates, 1,1,3,3- propane tetracarboxylates and 1,1,2,3-propane tetracarboxylates. Polycarboxylates containing sulfo substituents include the sulfosuccinate derivatives disclosed in British Patent Nos. 1,398,421 and 1,398,422 and in U.S. Patent No. 3,936,448, and the sulfonated pyrolysed citrates described in British Patent No. 1,082,179, while polycarboxylates containing phosphone substituents are disclosed in British Patent No. 1,439,000.

Alicyclic and heterocyclic polycarboxylates include cyclopentane-cis,cis,cis- tetracarboxylates, cyclopentadienide pentacarboxylates, 2,3,4,5-tetrahydro-furan - cis, cis, cis-tetracarboxylates, 2,5-tetrahydro-furan -cis - dicarboxylates, 2,2,5,5- tetrahydrofuran - tetracarboxylates, 1,2,3,4,5,6-hexane -hexacar-boxylates and and carboxymethyl derivatives of polyhydric alcohols such as sorbitol, mannitol and xylitol. Aromatic poly-carboxylates include mellitic acid, pyromellitic acid and the phthalic acid derivatives disclosed in British Patent No. 1,425,343.

Of the above, the preferred polycarboxylates are hydroxycarboxylates containing up to three carboxy groups per molecule, more particularly citrates.

Preferred builder systems for use in the present compositions include a mixture of a water-insoluble aluminosilicate builder such as zeolite A or of a layered silicate (SKS-6), and a water-soluble carboxylate chelating agent such as citric acid.

Preferred builder systems include a mixture of a water-insoluble aluminosilicate builder such as zeolite A, and a watersoluble carboxylate chelating agent such as citric acid. Preferred builder systems for use in liquid detergent compositions of the present invention are soaps and polycarboxylates.

Other suitable water-soluble organic salts are the homo- or co-polymeric acids or their salts, in which the polycarboxylic acid comprises at least two carboxyl radicals separated from each other by not more than two carbon atoms. Polymers of this type are disclosed in GB-A-1,596,756. Examples of such salts are polyacrylates of MW 2000-5000 and their copolymers with maleic anhydride, such copolymers having a molecular weight of from 20,000 to 70,000, especially about 40,000.

Detergency builder salts are normally included in amounts of from 5% to 80% by weight of the composition preferably from 10% to 70% and most usually from 30% to 60% by weight.

Bleaching agent Additional optional detergent ingredients that can be included in the detergent compositions of the present invention include bleaching agents such as hydrogen peroxide, PB1, PB4 and percarbonate with a particle size of 400-800 microns. These bleaching agent components can include one or more oxygen bleaching agents and, depending upon the bleaching agent chosen, one or more bleach activators. When present oxygen bleaching compounds will typically be present at levels of from about 1% to about 25%.

The bleaching agent component for use herein can be any of the bleaching agents useful for detergent compositions including oxygen bleaches as well as others known in the art. The bleaching agent suitable for the present invention can be an activated or non-activated bleaching agent.

Examples of suitable bleaching agents are disclosed in U.S. Patent Nos.

5,707,950 and 5,576,282.

The hydrogen peroxide releasing agents can be used in combination with, for example, the bleach activators disclosed in U.S. Patent No. 5,707,950 or Phenolsulfonate ester of N-nonanoyl-6-aminocaproic acid (NACA-OBS, described in WO94/28 106), which are perhydrolyzed to form a peracid as the active bleaching species, leading to improved bleaching effect. Also suitable activators are acylated citrate esters.

Useful bleaching agents, including peroxyacids and bleaching systems comprising bleach activators and peroxygen bleaching compounds for use in detergent compositions according to the invention are described in W095/27772, W095/27773, W095/27774, W095/27775 and U.S. Patent No. 5,707,950.

Metal-containing catalysts for use in bleach compositions, include cobalt- containing catalysts such as Pentaamine acetate cobalt(III) salts and manganese- containing catalysts such as those described in EPA 549 271; EPA 549 272; EPA 458 397; US 5,246,621; EPA 458 398; US 5,194,416 and US 5,114,611. Bleaching composition comprising a peroxy compound, a manganese-containing bleach catalyst and a chelating agent is described in the patent application No 94870206.3.

Dve transfer inhibition The detergent compositions of the present invention can also include compounds for inhibiting dye transfer from one fabric to another of solubilized and suspended dyes encountered during fabric laundering and conditioning operations involving colored fabrics.

Polymeric dye transfer inhibiting agents The detergent compositions according to the present invention can also comprise from 0.001% to 10 %, preferably from 0.01% to 2%, more preferably from 0.05% to 1% by weight of polymeric dye transfer inhibiting agents. Said polymeric dye transfer inhibiting agents are normally incorporated into detergentcompositions in order to inhibit the transfer of dyes from colored fabrics onto fabrics washed therewith. These polymers have the ability to complex or adsorb the fugitive dyes washed out of dyed fabrics before the dyes have the opportunity to become attached to other articles in the wash.

Especially suitable polymeric dye transfer inhibiting agents are polyamine N- oxide polymers, copolymers of N-vinylpyrrolidone and N-vinylimidazole, polyvinylpyrrolidone polymers, polyvinyloxazolidones and polyvinylimidazoles or mixtures thereof. Examples of such dye transfer inhibiting agents are disclosed in U.S. PatentNos. 5,707,950 and 5,707,951.

Additional suitable dye transfer inhibiting agents include, but are not limited to, cross-linked polymers. Cross-linked polymers are polymers whose backbone are interconnected to a certain degree; these links can be of chemical or physical nature, possibly with active groups n the backbone or on branches; cross-linked polymers have been described in the Journal of Polymer Science, volume 22, pages 1035- 1039.

In one embodiment, the cross-linked polymers are made in such a way that they form a three-dimensional rigid structure, which can entrap dyes in the pores formed by the three-dimensional structure. In another embodiment, the cross-linked polymers entrap the dyes by swelling.

Such cross-linked polymers are described in the co-pending European patent application 94870213.9 Addition of such polymers also enhances the performance of the enzymes according the invention.

Dispersants The detergent composition of the present invention can also contain dispersants. Suitable water-soluble organic salts are the homo- or co-polymeric acids or their salts, in which the polycarboxylic acid comprises at least two carboxyl radicals separated from each other by not more than two carbon atoms.

Polymers of this type are disclosed in GB-A-1,596,756. Examples of such salts are polyacrylates of MW 2000-5000 and their copolymers with maleic anhydride, such copolymers having a molecular weight of from 1,000 to 100,000.

Especially, copolymer of acrylate and methylacrylate such as the 480N having a molecular weight of 4000, at a level from 0.5-20% by weight of composition can be added in the detergentcompositions of the present invention.

The compositions of the invention may contain a lime soap peptiser compound, which has a lime soap dispersing power (LSDP), as defined hereinafter of no more than 8, preferably no more than 7, most preferably no more than 6. The lime soap peptiser compound is preferably present at a level from 0% to 20% by weight.

A numerical measure of the effectiveness of a lime soap peptiser is given by the lime soap dispersant power (LSDP) which is determined using the lime soap dispersant test as described in an article by H.C. Borghetty and C.A. Bergman, J.

Am. Oil. Chem. Soc., volume 27, pages 88-90, (1950). This lime soap dispersion test method is widely used by practitioners in this art field being referred to, for example, in the following review articles; W.N. Linfield, Surfactant science Series, Volume 7, page 3; W.N. Linfield, Tenside surf. det., volume 27, pages 159-163, (1990); and M.K. Nagarajan, W.F. Masler, Cosmetics and Toiletries, volume 104, pages 71-73, (1989). The LSDP is the % weight ratio of dispersing agent to sodium oleate required to disperse the lime soap deposits formed by 0.025g of sodium oleate in 30ml of water of 333ppm CaCo3 (Ca:Mg=3:2) equivalent hardness.

Surfactants having good lime soap peptiser capability will include certain amine oxides, betaines, sulfobetaines, alkyl ethoxysulfates and ethoxylated alcohols.

Exemplary surfactants having a LSDP of no more than 8 for use in accord with the present invention include C 16-C18 dimethyl amine oxide, C1 2-C18 alkyl ethoxysulfates with an average degree of ethoxylation of from 1-5, particularly C12- C15 alkyl ethoxysulfate surfactant with a degree of ethoxylation of amount 3 (LSDP=4), and the C14-C15 ethoxylated alcohols with an average degree of ethoxylation of either 12 (LSDP=6) or 30, sold under the tradenames Lutensol A012 and Lutensol A030 respectively, by BASF GmbH.

Polymeric lime soap peptisers suitable for use herein are described in the article by M.K. Nagarajan, W.F. Masler, to be found in Cosmetics and Toiletries, volume 104, pages 71-73, (1989).

Hydrophobic bleaches such as 4- [N-octanoyl-6-aminohexanoyl]benzene sulfonate, 4- [N-nonanoyl-6-aminohexanoyl]benzene sulfonate, 4-[N-decanoyl-6- aminohexanoyl]benzene sulfonate and mixtures thereof; and nonanoyloxy benzene sulfonate together with hydrophilic / hydrophobic bleach formulations can also be used as lime soap peptisers compounds.

Examples of other suitable dispersing agents are disclosed in U.S. Patent Nos.

5,576,282 and 5,728,671.

Conventional detergent enzvmes The detergent compositions can comprise in addition to the hexosaminidase enzyme one or more enzymes which provide cleaning performance and/or fabric care benefits.

Said enzymes include enzymes selected from hemicellulases, peroxidases, proteases, cellulases, xylanases, lipases, phospholipases, esterases, cutinases, pectinases, keratanases, reductases, oxidases, phenoloxidases, lipoxygenases, ligninases, pullulanases, tannases, pentosanases, malanases, 13-glucanases, arabinosidases, hyaluronidase, chondroitinase, laccase, and known amylases, or mixtures thereof.

Examples of suitable enzymes are disclosed in U.S. Patent Nos. 5,576,282, 5,728,671 and 5,707,950 A preferred combination is a detergent composition having cocktail of conventional applicable enzymes like protease, lipase, cutinase and/or cellulase in conjunction with the hexosaminidase.

Particularly useful proteases are described in PCT publications: WO 95/30010 published November 9, 1995 by The Procter & Gamble Company; WO 95/30011 published November 9, 1995 by The Procter & Gamble Company; and WO 95/29979 published November 9, 1995 by The Procter & Gamble Company.

In addition to the peroxidase enzymes disclosed in U.S. Patent Nos.

5,576,282, 5,728,671 and 5,707,950, other suitable peroxidase enzymes are disclosed in European Patent application EP No. 96870013.8, filed February 20, 1996. Also suitable is the laccase enzyme.

Preferred enhancers are substitued phenthiazine and phenoxasine 10- Phenothiazinepropionicacid (PPT), 1 0-ethylphenothiazine-4-carboxylic acid (EPC), 1 0-phenoxazinepropionic acid (POP) and 10-methylphenoxazine (described in WO 94/12621) and substitued syringates (C3-C5 substitued alkyl syringates) and phenols. Sodium percarbonate or perborate are preferred sources of hydrogen peroxide.

Said peroxidases are normally incorporated in the detergent composition at levels from 0.0001% to 2% of active enzyme by weight of the detergent composition.

Other preferred enzymes that can be included in the detergent compositions of the present invention include lipases. Suitable lipase enzymes for detergent usage include those produced by microorganisms of the Pseudomonas group, such as Pseudomonas stutzeri ATCC 19.154, as disclosed in British Patent 1,372,034.

Suitable lipases include those which show a positive immunological cross-reaction with the antibody of the lipase, produced by the microorganism Pseudomonas fluorescent IAM 1057. This lipase is available from Amano Pharmaceutical Co.

Ltd., Nagoya, Japan, under the trade name Lipase P "Amano," hereinafter referred to as "Amano-P". Other suitable commercial lipases include Amano-CES, lipases ex Chromobacter viscosum, e.g. Chromobacter viscosum var. lipolyticum NRRLB 3673 from Toyo Jozo Co., Tagata, Japan; Chromobacter viscosum lipases from U.S.

Biochemical Corp., U.S.A. and Disoynth Co., The Netherlands, and lipases ex Pseudomonas gladioli. Especially suitable lipases are lipases such as M1 LipaseR and LipomaxR (Gist-Brocades) and LipolaseR and Lipolase UltraK(Novo) which have found to be very effective when used in combination with the compositions of the present invention.

Also suitable are cutinases [EC 3.1.1.50] which can be considered as a special kind of lipase, namely lipases which do not require interfacial activation.

Addition of cutinases to detergent compositions have been described in e.g. WO 88/09367 (Genencor).

The lipases and/or cutinases are normally incorporated in the detergent composition at levels from 0.0001% to 2% of active enzyme by weight of the detergent composition.

Known amylases (a and/or B) can be included for removal of carbohydrate- based stains. WO 94/02597, Novo Nordisk A/S published February 03, 1994, describes cleaning compositions which incorporate mutant amylases. See also WO94/18314, Genencor, published August 18, 1994 and WO95/10603, Novo Nordisk A/S, published April 20, 1995. Other amylases known for use in detergent compositions include both cc- and -amylases. amylases are known in the art and include those disclosed in US Pat. 5,003,257; EP 252,666; WO 91/00353; FR 2,676,456; EP 285,123; EP 525,610; EP 368,341; and British Patent Specification No. 1,296,839 (Novo). Other suitable amylase are stability-enhanced amylases including Purafact Ox AmR described in WO 94/18314, published August 18, 1994 and W096/05295, Genencor, published February 22, 1996 and amylase variants from Novo Nordisk A/S, disclosed in WO 95/10603, published April 95.

Examples of commercial amylases products are TermamylB, Ban Fungamyl# and Duramyl#, all available from Nono Nordisk A/S Denmark.

W095/26397 describes other suitable amylases : amylases characterised by having a specific activity at least 25% higher than the specific activity of Termamyl at at a temperature range of 25°C to 55°C and at a pH value in the range of 8 to 10, measured by the Phadebas@ α-amylase activity assay. Other amylotytic enzymes with improved properties with respect to the activity level and the combination of thermostability and a higher activity level are described in W095/35382.

The above-mentioned enzymes may be of any suitable origin, such as vegetable, animal, bacterial, fungal and yeast origin. Purified or non-purified forms of these enzymes may be used. Also included by definition, are mutants of native enzymes. Mutants can be obtained e.g. by protein and/or genetic engineering, chemical and/or physical modifications of native enzymes. Common practice as well is the expression of the enzyme via host organisms in which the genetic material responsible for the production of the enzyme has been cloned.

Said enzymes are normally incorporated in the detergent composition at levels from 0.0001% to 2% of active enzyme by weight of the detergent composition. The enzymes can be added as separate single ingredients (prills, granulates, stabilized liquids, etc. containing one enzyme ) or as mixtures of two or more enzymes ( e.g. cogranulates).

Other suitable detergent ingredients that can be added are enzyme oxidation scavengers. Examples of such enzyme oxidation scavengers are ethoxylated tetraethylene polyamines.

A range of enzyme materials and means for their incorporation into synthetic detergent compositions is also disclosed in WO 9307263 and WO 9307260 to Genencor International, WO 8908694 to Novo, and U.S. 3,553,139, January 5, 1971 to McCarty et al. Enzymes are further disclosed in U.S. 4,101,457, Place et al, July 18, 1978, and in U.S. 4,507,219, Hughes, March 26, 1985. Enzyme materials useful for liquid detergent formulations, and their incorporation into such formulations, are disclosed in U.S. 4,261,868, Hora et al, April 14, 1981. Enzymes for use in detergents can be stabilized by various techniques. Enzyme stabilisation techniques are disclosed and exemplified in U.S. 3,600,319, August 17, 1971, Gedge et al, EP 199,405 and EP 200,586, October 29, 1986, Venegas. Enzyme stabilisation systems are also described, for example, in U.S. 3,519,570. A useful Bacillus, sp. AC13 giving proteases, xylanases and cellulases, is described in WO 9401532 to Novo.

Chelating Agents The detergent compositions herein may also optionally contain one or more iron and/or manganese chelating agents. Such chelating agents can be selected from the group consisting of amino carboxylates, amino phosphonates, polyfunctionally- substituted aromatic chelating agents and mixtures therein, all as hereinafter defined.

Without intending to be bound by theory, it is believed that the benefit of these materials is due in part to their exceptional ability to remove iron and manganese ions from washing solutions by formation of soluble chelates.

Examples of suitable chelating agents are disclosed in U.S. Patent No.

5,728,671.

The compositions herein may also contain water-soluble methyl glycine diacetic acid (MGDA) salts (or acid form) as a chelant or co-builder useful with, for example, insoluble builders such as zeolites, layered silicates and the like.

If utilized, these chelating agents will generally comprise from about 0.1% to about 15% by weight of the detergent compositions herein. More preferably, if utilized, the chelating agents will comprise from about 0.1% to about 3.0% by weight of such compositions.

Suds suppressor Another optional ingredient is a suds suppressor, exemplified by silicones, and silica-silicone mixtures. Examples of suitable suds suppressors are disclosed in U.S. Patent Nos. 5,707,950 and 5,728,671. These suds suppressors are normally employed at levels of from 0.001% to 2% by weight of the composition, preferably from 0.01% to 1% by weight.

Softening agents Fabric softening agents can also be incorporated into laundry detergent compositions in accordance with the present invention. These agents may be inorganic or organic in type. Inorganic softening agents are exemplified by the smectite clays disclosed in GB-A-1 400 898 and in USP 5,019,292. Organic fabric softening agents include the water insoluble tertiary amines as disclosed in GB-A1 514 276 and EP-B0 011 340 and their combination with mono C12-C14 quaternary ammonium salts are disclosed in EP-B-0 026 527 and EP-B-0 026 528 and di-long- chain amides as disclosed in EP-B-0 242 919. Other useful organic ingredients of fabric softening systems include high molecular weight polyethylene oxide materials as disclosed in EP-A-0 299 575 and 0 313 146.

Particularly suitable fabric softening agents are disclosed in U.S. Patent Nos.

5,707,950 and 5,728,673.

Levels of smectite clay are normally in the range from 2% to 20%, more preferably from 5% to 15% by weight, with the material being added as a dry mixed component to the remainder of the formulation. Organic fabric softening agents such as the water-insoluble tertiary amines or dilong chain amide materials are incorporated at levels of from 0.5% to 5% by weight, normally from 1% to 3% by weight whilst the high molecular weight polyethylene oxide materials and the water soluble cationic materials are added at levels of from 0.1% to 2%, normally from 0.15% to 1.5% by weight. These materials are normally added to the spray dried portion of the composition, although in some instances it may be more convenient to add them as a dry mixed particulate, or spray them as molten liquid on to other solid components of the composition.

Typical cationic fabric softening components include the water-insoluble quaternary-ammonium fabric softening actives, the most commonly used having been di-long alkyl chain ammonium chloride or methyl sulfate.

Preferred cationic softeners among these include the following: 1) ditallow dimethylammonium chloride (DTDMAC); 2) dihydrogenated tallow dimethylammonium chloride; 3) dihydrogenated tallow dimethylammonium methyl sulfate; 4) distearyl dimethylammonium chloride; 5) dioleyl dimethylammonium chloride; 6) dipalmityl hydroxyethyl methylammonium chloride; 7) stearyl benzyl dimethylammonium chloride; 8) tallow trimethylammonium chloride; 9) hydrogenated tallow trimethylammonium chloride; 10) C1 2-14 alkyl hydroxyethyl dimethylammonium chloride; 11) C1 2-18 alkyl dihydroxyethyl methylammonium chloride; 12) di(stearoyloxyethyl) dimethylammonium chloride (D S OEDMAC); 13) di(tallowoyloxyethyl) dimethylammonium chloride; 14) ditallow imidazolinium methylsulfate; 15) 1 -(2-tallowylamidoethyl)-2-tallowyl imidazolinium methylsulfate.

Biodegradable quaternary ammonium compounds have been presented as alternatives to the traditionally used di-long alkyl chain ammonium chlorides and methyl sulfates. Such quaternary ammonium compounds contain long chain alk(en)yl groups interrupted by functional groups such as carboxy groups. Said materials and fabric softening compositions containing them are disclosed in numerous publications such as EP-A-0,040,562, and EP-A-0,239,910.

Non-limiting examples of softener-compatible anions for the quaternary ammonium compounds and amine precursors include chloride or methyl sulfate.

Others Other components used in detergentcompositions may be employed, such as soil-suspending agents, soil-release agents, optical brighteners, abrasives, bactericides, tarnish inhibitors, coloring agents, and/or encapsulated or non- encapsulated perfumes, examples of which are disclosed in U.S. Patent Nos.

5,707,950, 5,576,282 and 5,728,671.

Is is well known in the art that free chlorine in tap water rapidly deactivates the enzymes comprised in detergent compositions. Therefore, using chlorine scavenger such as perborate, ammonium sulfate, sodium sulphite or polyethyleneimine at a level above 0.1% by weight of total composition, in the formulas will provide improved through the wash stability of the detergent enzymes.

Compositions comprising chlorine scavenger are described in the European patent application 92870018.6 filed January 31, 1992.

Alkoxylated polycarboxylates such as those prepared from polyacrylates are useful herein to provide additional grease removal performance. Such materials are described in WO 91/08281 and PCT 90/01815 at p. 4 et seq., incorporated herein by reference. Chemically, these materials comprise polyacrylates having one ethoxy side-chain per every 7-8 acrylate units. The side-chains are of the formula -(CH2CH2O)m(CH2)nCH3 wherein m is 2-3 and n is 6-12. The side-chains are ester-linked to the polyacrylate "backbone" to provide a "comb" polymer type structure. The molecular weight can vary, but is typically in the range of about 2000 to about 50,000. Such alkoxylated polycarboxylates can comprise from about 0.05% to about 10%, by weight, of the compositions herein.

Method of washing The compositions of the invention may be used in essentially any washing or cleaning methods, including soaking methods, pretreatment methods and methods with rinsing steps for which a separate rinse aid composition may be added.

The process described herein comprises contacting fabrics with a laundering solution in the usual manner and exemplified hereunder.

The process of the invention is conveniently carried out in the course of the cleaning process. The method of cleaning is preferably carried out at 50C to 950C, especially between 10"C and 60°C. The pH of the treatment solution is preferably from 7 to 11.

The following examples are meant to exemplify compositions of the present invention, but are not necessarily meant to limit or otherwise define the scope of the invention. In the detergent compositions, the enzyme levels are expressed by pure enzyme by weight of the total composition and unless otherwise specified, the detergent ingredients are expressed by weight of the total compositions. The abbreviated component identifications herein have the following meanings: LAS Sodium linear C12 alkyl benzene sulphonate TAS Sodium tallow alkyl sulphate CXYAS : Sodium C1 - Cloy alkyl sulfate 25EY : A C12-C15 predominantly linear primary alcohol condensed with an average of Y moles of ethylene oxide <BR> <BR> <BR> <BR> <BR> <BR> CXYEZ : A C1 - C1 Y predominantly linear primary alcohol condensed with an average of Z moles of ethylene oxide XYEZS : C1X - C1Y sodium alkyl sulfate condensed with an average of Z moles of ethylene oxide per mole QAS : R2.N+(CH3)2(C2H4OH) with R2 = C12-C14 Soap Sodium linear alkyl carboxylate derived from a 80/20 mixture of tallow and coconut oils.

Nonionic # C13-C15 mixed ethoxylated/propoxylated fatty alcohol with an average degree of ethoxylation of 3.8 and an average degree of propoxylation of 4.5 sold under the tradename Plurafac LF404 by BASF Gmbh.

CFAA C1 C12-C14 alkyl N-methyl glucamide TFAA C1 6-C18 alkyl N-methyl glucamide.

TPKFA . C12-C14 topped whole cut fatty acids.

DEQA Di-(tallow-oxy-ethyl) dimethyl ammonium chloride.

Neodol 45-13 C14-C15 linear primary alcohol ethoxylate, sold by Shell Chemical CO.

Silicate : Amorphous Sodium Silicate (SiO2:Na2O ratio = 2.0) NaSKS-6 : Crystalline layered silicate of formula #-Na2Si2O5 Carbonate : Anhydrous sodium carbonate with a particle size between 200 µm and 900µm.

Bicarbonate : Anhydrous sodium bicarbonate with a particle size between 400 µm and 1200µm.

STPP : Anhydrous sodium tripolyphosphate MA/AA Copolymer of 1:4 maleic/acrylic acid, average molecular weight about 70,000-80,000 Zeolite A Hydrated Sodium Aluminosilicate of formula Na12(AlO2SiO2)12 27H20 having a primary particle size in the range from 0.1 to 10 micrometers Citrate Tri-sodium citrate dihydrate of activity 86,4% with a particle size distribution between 425 µm and 850 µm.

Citric Anhydrous citric acid Pub 1 Anhydrous sodium perborate monohydrate bleach, empirical formula NaBO2.H2O2 PB4 : Anhydrous sodium perborate tetrahydrate Percarbonate Anhydrous sodium percarbonate bleach of empirical formula 2Na2CO3.3H2O3 TAED : Tetraacetyl ethylene diamine.

NOBS : Nonanoyloxybenzene sulfonate in the form of the sodium salt.

Photoactivated Bleach. Sulfonated zinc phtalocyanine encapsulated in dextrin soluble polymer.

Protease : Proteolytic enzyme sold under the tradename Savinase, Alcalase, Durazym by Novo Nordisk A/S, Maxacal, Maxapem sold by Gist-Brocades and proteases described in patents WO91/06637 and/or WO95/10591 and/or EP 251 446.

Amylase Amylolytic enzyme sold under the tradename Purafact Ox AmR described in WO 94/18314, W096/05295 sold by Genencor; Termamyl#, Fungamyl# and Duramyl#, all available from Novo Nordisk A/S and those described in W095/26397.

Lipase # Lipolytic enzyme sold under the tradename Lipolase, Lipolase Ultra by Novo Nordisk A/S Hexosaminidase A hexosaminidase according to the present invention compositions, having MIC less than about 0. 125%.

Cellulase Cellulytic enzyme sold under the tradename Carezyme, Celluzyme and/or Endolase by Novo Nordisk A/S.

CMC Sodium carboxymethyl cellulose.

HEDP 1,1 -hydroxyethane diphosphonic acid.

DETPMP : Diethylene triamine penta (methylene phosphonic acid), marketed by Monsanto under the Trade name Dequest 2060.

PVNO Poly Poly(4-vinylpyridine)-N-Oxide.

PVPVI : Poly (4-vinylpyridine)-N-oxide/copolymer of vinyl-imidazole and vinyl-pyrrolidone.

Brightener 1 Disodium 4,4'-bis(2-sulphostyryl)biphenyl.

Brightener 2. Disodium 4,4'-bis(4-anilino-6-morpholino- 1.3. 5-triazin-2-yl) stilbene-2 :2'-disulfonate.

Silicone antifoam Polydimethylsiloxane foam controller with siloxane- oxyalkylene copolymer as dispersing agent with a ratio of said foam controller to said dispersing agent of 10 : 1 to 100:1.

Granular Suds 12% Silicone/silica, 18% stearyl alcohol,70% starch in Suppressor granular form SRP 1 Sulfobenzoyl or sodium isethionate end capped esters with oxyethylene oxy and terephtaloyl backbone.

SRP 2 : Diethoxylated poly (1,2 propylene terephtalate) short block polymer.

Sulphate Anhydrous sodium sulphate.

HMWPEO High molecular weight polyethylene oxide Example 1 The following detergent formulations, according to the present invention are prepared, where I and III are phosphorus-containing detergent compositions, and II is a zeolite-containing detergent composition: I II III Blown Powder: STPP 24.0 # 24.0 Zeolite A - 24.0 C45AS 9.0 6.0 13.0 MA/AA 2.0 4.0 2.0 LAS 6.0 8.0 11.0 TAS 2.0 - Silicate 7.0 3.0 3.0 CMC 1.0 1.0 0. 5 Brightener 2 0.2 0.2 0.2 Soap 1.0 1.0 1.0 DETPMP 0.4 0.4 0.2 Spray On C45E7 2.5 2.5 2.0 C25E3 2.5 2.5 2.0 Silicone antifoam 0.3 0.3 0.3 Perfume 0.3 0.3 0.3 Dry additives: Carbonate 6.0 13.0 15.0 PB4 18.0 18.0 10.0 PB1 4.0 4.0 0 TAED 3.0 3.0 1.0 Photoactivated bleach 0.02 0.02 0.02 Protease 0.01 0.01 0.01 Lipase 0.009 0.009 Amylase 0.002 -- 0.001 Hexosaminidase 0.05 0.01 0.001 Dry mixed sodium sulfate 3.0 3.0 5.0 Balance (Moisture & 100.0 100.0 100.0 Miscellaneous) Density (g/litre) 630 670 670 Example 2 The following nil bleach-containing detergent formulations of particular use in the washing of colored clothing, according to the present invention are prepared: I II III Blown Powder Zeolite A 15.0 15.0 Sodium sulfate 0.0 5.0 LAS 3.0 3.0 DETPMP 0.4 0.5 CMC 0.4 0.4 MA/AA 4.0 4.0 Agglomerates C45AS 11.0 LAS 6.0 5.0 TAS 3.0 2.0 Silicate 4.0 4.0 Zeolite A 10.0 15.0 13.0 CMC 0.5 MA/AA 2.0 Carbonate 9.0 7.0 7.0 Spray On Perfume 0.3 0.3 0.5 C45E7 4.0 4.0 4.0 C25E3 2.0 2.0 2.0 Dry additives MA/AA 3.0 NaSKS-6 12.0 Citrate 10.0 8.0 Bicarbonate 7.0 3.0 5.0 Carbonate 8.0 5.0 7.0 PVPVI/PVNO 0.5 0.5 0.5 Protease 0.026 0.016 0.047 Lipase 0.009 -- 0.009 Amylase 0.005 0.005 -- Hexosaminidase 0.05 0.01 0.001 Cellulase 0.006 0.006 Silicone antifoam 5.0 5.0 5.0 Dry additives Sodium sulfate 0.0 9.0 0.0 Balance (Moisture and 100.0 100.0 100.0 Miscellaneous) Density (g/litre) 700 700 700 Example 3 The following detergent formulations, according to the present invention are prepared: I II III IV LAS 20.0 14.0 24.0 22.0 QAS 0.7 1.0 - 0.7 TFAA - 1.0 - - C25E5/C45E7 - 2.0 - 5 C45E3S - 2.5 - - STPP 30.0 18.0 30.0 22.0 Silicate 9.0 5.0 10.0 8.0 Carbonate 13.0 7.5 - 5.0 Bicarbonate - 7.5 - - DETPMP 0.7 1.0 - - SRP 1 0.3 0.2 - 0.1 MA/AA 2.0 1.5 2.0 1.0 CMC 0.8 0.4 0.4 0.2 Hexosaminidase 0.05 0.01 0.001 0.05 Protease 0.008 0.01 0.026 0.026 Amylase 0.007 -- 0.005 0.002 Lipase 0.004 -- -- 0.002 Cellulase 0.0015 0.0005 - - Photoactivated bleach 70ppm 45ppm - 1 0ppm Brightener 1 0.2 0.2 0.08 0.2 PB1 6.0 2.0 - - NOBS 2.0 1.0 Balance (Moisture and 100 100 100 100 Miscellaneous) Example 4 The following liquid detergent formulations, according to the present invention are prepared: I II III IV V VI VII VIII LAS 10.0 13.0 9.0 - 25.0 - - - C25AS 4.0 1.0 2.0 10.0 - 13.0 18.0 15.0 C25E3S 1.0 - - 3.0 - 2.0 2.0 4.0 C25E7 6.0 8.0 13.0 2.5 - - 4.0 4.0 TFAA - - - 4.5 - 6.0 8.0 8.0 QAS - - - - 3.0 1.0 - - TPKFA 2.0 - 13.0 2.0 - 15.0 7.0 7.0 Rapeseed fatty - - - 5.0 - - 4.0 4.0 acids Citric 2.0 3.0 1.0 1.5 1.0 1.0 1.0 1.0 Dodecenyl/ 12.0 10.0 - - 15.0 - - - tetradecenyl succinic acid Oleic acid 4.0 2.0 1.0 - 1.0 - - - Ethanol 4.0 4.0 7.0 2.0 7.0 2.0 3.0 2.0 1,2 4.0 4.0 2.0 7.0 6.0 8.0 10.0 13.- Propanediol Mono Ethanol - - - 5.0 - - 9.0 9.0 Amine Tri Ethanol - - 8 - - - - - Amine NaOH (pH) 8.0 8.0 7.6 7.7 8.0 7.5 8.0 8.2 Ethoxylated 0.5 - 0.5 0.2 - - 0.4 0.3 tetraethylene pentamine DETPMP 1.0 1.0 0.5 1.0 2.0 1.2 1.0 0 SRP 2 0.3 - 0.3 0.1 - - 0.2 0.1 PVNO - - - - - - - 0.10 Hexosaminidase 0.05 0.01 0.001 0.05 0.01 0.001 0.05 0.05 Protease .005 .005 .004 .003 0.08 .005 .003 .006 Lipase .002 - .0002 - - .003 .003 Amylase .002 -- -- .004 .002 .008 .005 .005 Cellulase - - - .0001 - - .0004 .0004 Boric acid 0.1 0.2 - 2.0 1.0 1.5 2.5 2.5 Na formate 1.0 Ca chloride - 0.015 - 0.01 - - - - Bentoniteclay - - - - 4.0 4.0 - - Suspending - - - - 0.6 0.3 3 clay SD3 Balance 100 100 100 100 100 100 100 100 Moisture and Miscellaneous Example 5 Granular fabric detergent compositions which provide "softening through the wash" capability are prepared in accord with the present invention: I II 45AS - 10.0 LAS 7.6 - 68AS 1. 3 45E7 4.0 0 25E3 - 5.0 Coco-alkyl-dimethyl hydroxy- 1.4 1.0 ethyl ammonium chloride Citrate 5.0 3.0 Na-SKS-6 - 11.0 Zeolite A 15.0 15.0 MA/AA 4.0 4.0 DETPMP 0.4 0.4 PB1 15.0 - Percabonate 15.0 TAED 5.0 5.0 Smectite clay 10.0 5.0 HMWPEO - 0.1 Hexosaminidase 0.05 0.01 Protease 0.02 0.01 Lipase 0.02 0.01 Amylase 0.01 0.005 Cellulase 0.001 - Silicate 3.0 5.0 Carbonate 10.0 10.0 Granular suds suppressor 1.0 4.0 CMC 0.2 0.1 Water/minors Up to 100% Example 6 Syndet bar fabric detergent compositions are prepared in accord with the present invention: I II III IV C26AS 20.00 20.00 20.00 20.00 CFAA 5.0 5.0 5.0 5.0 LAS (C11-13) 10.0 10.0 10.0 10.0 Sodium carbonate 25.0 25.0 25.0 25.0 Sodium pyrophosphate 7.0 7.0 7.0 7.0 STPP 7.0 7.0 7.0 7.0 Zeolite A 5.0 5.0 5.0 5.0 CMC 0.2 0.2 0.2 0.2 Polyacrylate (MW 1400) 0.2 0.2 0.2 0.2 Coconut monethanolamide 5.0 5.0 5.0 5.0 Hexosaminidase 0.05 0.01 0.001 0.05 Amylase 0.01 -- 0.005 -- Protease 0.3 0.5 0.05 Brightener, perfume 0.2 0.2 0.2 0.2 CaS04 1.0 1.0 1.0 1.0 MgSO4 1.0 1.0 1.0 1.0 Water 4.0 4.0 4.0 4.0 Filler* : balance to 100% *Can be selected from convenient materials such as CaC03, talc, clay (Kaolinite, Smectite), silicates, and the like.

Example 7 Weight % Ingredients A B STPP 24.0 45 Sodium carbonate 20.0 13.5 Silicate 15.0 13.5 Nonionic surfactants 2.0 2.0 MA/AA 4.0 -- Protease 0.083 0.083 Amylase 0.005 0.005 Hexosaminidase 0.01 0.05 PB1 14.5 14.5 Cobalt catalyst* 0.008 -- Dibenzoyl peroxide (18% active) 4.4 4.4 Water, sodium sulfate and misc. Balance Balance *Pentaamineacetatocobalt (III) nitrate.

Example 8 Light-duty liquid dishwashing detergent formulae are prepared as follows: Composition Ingredient A B C % Weight Surfactant 32.00 29.50 30.75 Ethanol 4.00 4.00 4.00 Ammonium citrate 0.06 0.06 0.06 Magnesium chloride 3.32 3.32 3.32 Ammonium sulfate 0.08 0.08 0.08 Hydrogen peroxide 200pom -- -- Perfume 0.18 0.18 0.18 Protease 0.005 0.005 0.005 Amylase 0.005 0.005 0.005 Hexosaminidase 0.05 0.05 0.05 Water and minors Balance Balance Balance SEQUENCE LISTING (1) GENERAL INFORMATION: (i) APPLICANT: ANDRE CHRISTIAN CONVENTS ROSA LAURA MOESE ANN MARGARET WOLFF (ii) TITLE OF INVENTION: LAUNDRY AND CLEANING COMPOSITIONS CONTAINING HEXOSAMINIDASE ENZYMES (iii) NUMBER OF SEQUENCES: 11 (iv) CORRESPONDENCE ADDRESS: (A) ADDRESSEE: THE PROCTER & GAMBLE COMPANY (B) STREET: 11810 East Miami River Road (C) CITY: CINCINNATI (D) STATE: OHIO (E) COUNTRY: USA (F) ZIP: 45253-8707 (v) COMPUTER READABLE FORM: (A) MEDIUM TYPE: Diskette (B) COMPUTER: IBM PC compatible (C) OPERATING SYSTEM: PC-DOS/MS-DOS (D) SOFTWARE: PatentIn Release &num 1.0, Version &num 1.25 (vi) CURRENT APPLICATION DATA: (A) APPLICATION NUMBER: (B) FILING DATE: August 19, 1997 (C) CLASSIFICATION: (viii) ATTORNEY/AGENT INFORMATION: (A) NAME: ZERBY, KIM WILLIAM (B) REGISTRATION NUMBER: 32,323 (C) REFERENCE/DOCKET NUMBER: Case 6616P2 (ix) TELECOMMUNICATION INFORMATION: (A) TELEPHONE: 513-627-2885 (B) TELEFAX: 513-627-0318 (2) INFORMATION FOR SEQ ID NO:1: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 611 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 1: 1 MNYRIDFAVL SEHPQFCRFG LTLHNLSDQD LKAWSLHFTI DRYIQPDSIS 51 HSQIHQVGSF CSLTPEQDVI NSNSHFYCEF SIKTAPFPFH YYTDGIKAAF 101 VQINDVEPRV RHDVIVTPIA LASPYRERSE IPATDAATLS LLPKPNHIER 151 LDGEFALTAG SQISLQSSCA ETAATWLKQE LTHLYQWQPH DIGSADIVLR 201 TNPTLDEGAY LLSVDRKPIR LEASSHIGFV HASATLLQLV RPDGDNLLVP 251 HIVIKDAPRF KYRGMMLDCA RHFHPLERVK RLINQLAHYK FNTFHWHLTD 301 DEGWRIEIKS LPQLTDIGAW RGVDEVLEPQ YSLLTEKHGG FYTQEEIREV 351 IAYAAERGIT VIPEIDIPGH SRAAIKALPE WLFDEDDQSQ YRSIQYYNDN 401 VLSPALPGTY RFLDCVLEEV AALFPSHFIH IGADEVPDGV WVNSPKCQAL 451 MAEEGYTDAK ELQGHLLRYA EKKLKSLGKR MVGWEEAQHG DKVSKDTVIY 501 SWLSEQAALN CARQGFDVIL QPGQFTYLDI AQDYAPEEPG VDWAGVTPLE 551 RAYRYEPLVE VPEHDPLRKR ILGIQCALWC ELVNNQDRMD YMIYPRLTAL 601 AGSGLDTKIP A 2) INFORMATION FOR SEQ ID NO: 2: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 430 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 2: 1 PRFPYRGIFL DVARNFHKKD AVLRLLDQMA AYKLNKFHFH LSDDEGWRIE 51 IPGLPELTEV GGQRCHDLSE TTCLLPQYGQ GPDVYGGFFS RQDYIDIIKY 101 AQARQIEVIP EIDMPAHARA AWSMEARYK KLHAAGKEQE ANEFRLVDPT 151 DTSNTTSVQF FNRQSYLNPC LDSSQRFVDK VIGEIAQMHK EAGQPIKTWH 201 FGGDEAKNIR LGAGYTDKAK PEPGKGIIDQ SNEDKPWAKS QVCQTMIKEG 251 KVADMEHLPS YFGQEVSKLV KAHGIDRMQA WQDGLKDAES SKAFATSRVG 301 VNFWDTLYWG GFDSVNDWAN KGYEWVSNP DYVYMDFPYE VNPDERGYYW 351 GTRFSDERKV FSFAPDNMPQ NAETSVDRDG NHFNAKSDKP WPGAYGLSAQ 401 LWSETQRTDP QMEYMIFPRA LSVAERSWHR 2) INFORMATION FOR SEQ ID NO: 3: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 777 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 3: 1 MKRLTFGACI CCLLSLMACS QKAKQVQIPE YDKGINIIPL PMQLTESDDS 51 FEVDDKTTIC VSAEELKPIA KLLADKLRAS ADLSLQIEIG EEPSGNAIYI 101 GVDTALPLKE EGYMLRSDKR GVSIIGKSAH GAFYGMQTLL QLLPAEVESS 151 NEVLLPMTVP GVEIKDEPAF GYRGFMLDVC RHFLSVEDIK KHIDIMAMFK 201 INRFHWHLTE DQAWRIEIKK YPRLTEVGST RTEGDGTQYS GFYTQEQVRD 251 IVQYASDHFI TVIPMIEMPG HAMAALAAYP QFRCFPREFK PRIIWGVEQD 301 VYCAGKDSVF RFISDVIDEV APLFPGTYFH IGGDECPKDR WKACSLCQKR 351 MRDNGLKDEH ELQSYFIKQA EKVLQKHGKR LIGWDEILEG GLAPSATVMS 401 WRGEDGGIAA ANMNHDVIMT PGSGGLYLDH YQGDPTVEPV AIGGYAPLEQ 451 VYAYNPLPKE LPADKHRYVL GAQANLWAEY LYTSERYDYQ AYPRLLAVAE 501 LTWTPLAKKD FADFCRRLDN ACVRLDMHGI NYHIPLPEQP GGSSDFIAFT 551 DKAKLTFTTS RPMKMVYTLD ETEPSLTSTP YTVPLEFAQT GLLKIRTVTA 601 GGKMSPVRRI RVEKQPFNMS MEVPAPKPGL TIRTAYGDLY DVPDLQQVAS 651 WEVGTVSSLE EIMHGKEKIT SPEVLERRW EATGYVLIPE DGVYEFSTEN 701 NEFWIDNVKL IDNVGEVKKF SRRNSSRALQ KGYHPIKTIW VGAIQGAWPT 751 YWNYSRVMIR LKGEEKFKPI SSDMLFQ 2) INFORMATION FOR SEQ ID NO: 4: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 562 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 4: 1 MVLDKMIIFH LLLWLCNVW HAAKVEILPA PQSVTWENDT AIIINPRLLQ 51 ANTSCPLLED AFVRTVSAIE KLKWHPFPID DFNTANGKNI KTSLVHIQVD 101 DATVDLQLGV NESYTLKINT DGINIHAATT WGALHGLVSL QQLIIHTSED 151 KYWPLSVTI SDFPNFKHRG LMIDSGRNFL TVDSILEQID IMALSKMNSL 201 HWHLADSQSW PVALESYPHM IKDAYSNDEV YSKNDLKYIV DYARARGVRV 251 IPEIDMPGHA RAGWKQVDPT IVECADAFWT DAAVEPPPGQ LNIESEKTYE 301 VISNVYNELS DIFIDDVFHV GNDELQEKCY SAQLLPNNTV TDLLKRYLKK 351 ALPIFNKVNH RKLTMWDDVL LSDVSADKIP SNITLQVWHE ISGVKNLTSR 401 GYDVWSLSD FLYLDCGNAG WVTNDPRYVE TPENVDFNTG QGGSWCGPYK 451 SYQRIYNFDF TANLTETEKN HVLGREAALW SEQVDSTVLT TKIWPRTAAL 501 AELTWSGNKD SNGHHRGYEF TQRILNFREY LVKLGYGVSP LVPKYCLLNP 551 HACDLYKNPP VY 2) INFORMATION FOR SEQ ID NO: 5: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 847 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 5: 1 MASDIDQKDV DYAAKNLKLT TSLVANKPKD CPPEAPWGAC YRVEINLENT 51 GSKSLNENVE IYFSSIHRTL GSKSEEFKVE HINGDLHKIT TTEKFKGLKG 101 GKTKSFQVDF MNWIVSNSDF MPNYYVASEH LEGRNILNTV PIDAVHITEE 151 VSGFTTGIKH TPNQLKRTAN DLLPAATATT RYEQYSKVKD LGADAVSAHI 201 LPTPLETSVH EGSLNIAQGI NIVSDALPAD QVEALNFRFE TLGVNTGTGV 251 PVNVTIKADS SKKSGSYTLD VTSSGIRIVG VDKAGAFYGV QSLAGLVTVG 301 KDTINQVSIN DEPRLDYRGM HMDVSRNFHS KELVFRFLDQ MAAYKMNKFH 351 FHLADDEGWR LEINGLPELT QVGAHRCHDV EQNKCMMPQL GSGAELPNNG 401 SGYYTREDYK EILAYASARN IQVIPSMDMP GHSLAAVKSM EARYRKFMAE 451 GDWKAEMYL LSDPNDTTQY YSIQHYQDNT INPCMESSFV FMDKVIDEIN 501 KLHKEGGQPL TDYHIGADET AGAWGDSPEC RKMFVAPESG VKNAKDINGY 551 FINRISHILD AKGLTLGAWN DGLSHKALDA SSLAGNPPKA WVWGTMFWGG 601 VDQYNSFANK GYDWVTPPD AYYFDMPYEN DPEERGYYWA TRFNDTKKVF 651 SFMPENVPAN VEWMTDRMGA KISATTGEKT HDFLGVQGAL WSETIRTDAQ 701 VEYMVLPRMI AVAERGWHKA SWEEEHKEGI TYTSNVDGHE GTTHLNDNIA 751 TRDADWAHFS NILGYKEMPK LDKAGITYRL PVLGAVIKNN ILDWTEFHG 801 VAIQYSLDGK TWHKYDDTKK PQVSTKALVR SVSTNGRTGR AVEVLAK 2) INFORMATION FOR SEQ ID NO: 6: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 1589 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: cDNA (iii) HYPOTHETICAL: NO (iii) ANTI-SENSE: NO (v) FRAGMENT TYPE: internal (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 6: 1 atgacaagct ccaggctttg gttttcgctg ctgctggcgg cagcgttcgc 51 aggacgggcg acggccctct ggccctggcc tcagaacttc caaacctccg 101 accagcgcta cgtcctttac ccgaacaact ttcaattcca gtacgatgtc 151 agctcggccg cgcagcccgg ctgctcagtc ctcgacgagg ccttccagcg 201 ctatcgtgac ctgcttttcg gttccgggtc ttggccccgt ccttacctca 251 cagggaaacg gcatacactg gagaagaatg tgttggttgt ctctgtagtc 301 acacctggat gtaaccagct tcctactttg gagtcagtgg agaattatac 351 cctgaccata aatgatgacc agtgtttact cctctctgag actgtctggg 401 gagctctccg aggtctggag acttttagcc agcttgtttg gaaatctgct 451 gagggcacat tctttatcaa caagactgag attgaggact ttccccgctt 501 tcctcaccgg ggcttgctgt tggatacatc tcgccattac ctgccactct 551 ctagcatcct ggacactctg gatgtcatgg cgtacaataa attgaacgtg 601 ttccactggc atctggtaga tgatccttcc ttcccatatg agagcttcac 651 ttttccagag ctcatgagaa aggggtccta caaccctgtc acccacatct 701 acacagcaca ggatgtgaag gaggtcattg aatacgcacg gctccggggt 751 atccgtgtgc ttgcagagtt tgacactcct ggccacactt tgtcctgggg 801 accaggtatc cctggattac tgactccttg ctactctggg tctgagccct 851 ctggcacctt tggaccagtg aatcccagtc tcaataatac ctatgagttc 901 atgagcacat tcttcttaga agtcagctct gtcttcccag attttatctt 951 catcttggag gagatgaggt tgatttcacc tgctggaagt ccaacccaga 1001 gatccaggac tttatgagga agaaaggctt cggtgaggac ttcaagcagc 1051 tggagtcctt ctacatccag acgctgctgg acatcgtctc ttcttatggc 1101 aagggctatg tggtgtggca ggaggtgttt gataataaag taaagattca 1151 gccagacaca atcatacagg tgtggcgaga ggatattcca gtgaactata 1201 tgaaggagct ggaactggtc accaaggccg gcttccgggc ccttctctct 1251 gccccctggt acctgaaccg tatatcctat ggccctgact ggaaggattt 1301 ctacgtagtg gaacccctgg catttgaagg tacccctgag cagaaggctc 1351 tggtgattgg tggagaggct tgtatgtggg gagaatatgt ggacaacaca 1401 aacctggtcc ccaggctctg gcccagagca ggggctgttg ccgaaaggct 1451 gtggagcaac aagttgacat ctgacctgac atttgcctat gaacgtttgt 1501 cacacttccg ctgtgagttg ctgaggcgag gtgtccaggc ccaacccctc 1551 aatgtaggct tctgtgagca ggagtttgaa cagacctga 2) INFORMATION FOR SEQ ID NO: 7: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 3670 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: cDNA (iii) HYPOTHETICAL: NO (iii) ANTI-SENSE: NO (v) FRAGMENT TYPE: internal (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 7: 1 ggtggtggca cctcctgccg cgcggtattc ggcatgcgtc cggcgtttga 51 ttggcgacag gaccggcagc gccaacctgt tgcttggcgt ggaacgcgat 101 ggacgccgtc attcacgcca tcaccttagc tgccgaacaa ggcggcctga 151 ataacgataa ctttggtcaa ctgcacgtgg gcttggcgct ggctggcgtg 201 agcaccaagc gacttggcat gctttatgca attgccacac cgtttgcgtc 251 gctcacgctc aataccgatg cctatggtgc gtgcctcggt gcgcaccacg 301 gtgacaacgg cgccatcatg attgctggca cgggctcatg cggtttgttc 351 ttgcaagacg gccaccagca cgtggtgggg ggacgtgagt tcccgatctc 401 cgatgagggc agtggcgcgg tgatgggact gcgcctgatt caacaagtgc 451 tgctgattga agatggtatt tatccggcca cgccacttag tcagtgtgtc 501 atgcagcatt gacacgatgt gacgccattg tcgcttggtc gaaatccgct 551 ttacctcgcg actatggtca attttcgccg cagattttcg cgttggcgaa 601 tcaaggtgac acgctagcaa tatccctgct gaaacagaca gcagcggata 651 tcgaaatgtt tttgaacgcc ctgcatcgca aaggggcaca gcgaatctgc 701 ttcatgggca gcatcgcgga acgcattcac gcatggttat cccctcccgt 751 tcagcaatgg atcgtcgcac cgcaagcgga tgcgatggag ggcgcattaa 801 tgtttgccgg caaagccgag cataatttgt attaagggtt gctcatgaac 851 tatcgaatag acttcgcggt attgtcagaa catccacagt tctgccgttt 901 tggcttgacg ctgcataacc tcagcgatca ggacttaaag gcctggagcc 951 tgcatttcac catcgatcgc tacattcagc ccgatagcat cagtcacagc 1001 cagattcatc aagtcggcag tttctgttcg ctcacgccgg agcaggacgt 1051 gataaattcc aacagccatt tctactgcga attcagcatc aaaaccgcgc 1101 cgtttccgtt tcactattac accgacggca tcaaagccgc gtttgtccaa 1151 attaatgatg tagagccgcg ggttcgtcac gacgtgatcg tcacccccat 1201 cgcactcgcc tccccctatc gggaacgcag cgagatcccg gccacggatg 1251 ccgcgacgtt gagcctgtta cccaaaccca atcatatcga acgcttggat 1301 ggtgaatttg cccttaccgc cggcagccag atttcattgc aatcctcttg 1351 tgcagaaact gccgccacgt ggctcaagca agaactgacg catctctatc 1401 agtggcagcc acacgatatt ggcagcgccg acattgtgct acgcaccaac 1451 ccaacgctgg atgaaggcgc ctatctgctg tcagtcgacc gcaaacctat 1501 tcgtttggaa gccagcagtc acatcggctt tgtccatgcc agtgcgacat 1551 tgctgcaatt ggttcgccca gatggcgaca acctgctggt gccacacatc 1601 gttatcaaag acgcaccgcg ctttaaatac cgcggcatga tgctggattg 1651 cgcgcgtcat tttcatccgc tggagcgcgt taaacgcctc atcaaccaac 1701 tggcgcatta caaattcaac acctttcatt ggcatctgac cgatgatgaa 1751 ggttggcgca ttgaaattaa gtctctacct caattgaccg acattggcgc 1801 gtggcgcggt gtggatgaag tcctggaacc gcaatacagc ctgctgaccg 1851 aaaaacacgg tggcttttac acccaagagg agatccgtga agtgatcgcc 1901 tacgccgcag aacgcggcat cacggtgatt ccagaaattg acattcccgg 1951 tcacagccga gcggcgatca aagccttacc ggaatggcta tttgacgaag 2001 atgaccaatc acaataccgc agcattcagt actacaacga caacgtgcta 2051 tcgccagccc tgcccggcac ctaccgtttt ctcgattgcg tattggagga 2101 agtggccgcg ctgtttccga gccatttcat tcacattggc gccgatgaag 2151 tgccagatgg cgtgtgggtc aacagcccga aatgtcaggc attgatggca 2201 gaagagggct acaccgacgc caaagagtta caagggcacc tgctgcgcta 2251 tgcggagaag aagctcaaat cactcggcaa acgcatggtc ggttgggaag 2301 aagcgcagca tggtgacaaa gtcagcaaag ataccgtgat ttattcttgg 2351 ttatccgaac aagccgcact gaactgcgcc cgtcaagggt ttgatgtcat 2401 tttacaaccg ggacagttta cgtacctcga cattgcgcaa gactacgcgc 2451 cagaagagcc gggcgtcgac tgggctggcg tgacgccact ggagcgcgcc 2501 tatcgctacg agccgctggt cgaggtgcca gaacacgacc cgctgcgcaa 2551 acgcattttg gggattcagt gcgcgctgtg gtgtgaactg gtcaacaatc 2601 aagaccgcat ggactacatg atctatccgc gtttgaccgc actggcggga 2651 agcggcttgg acacaaaaat cccagcgtga ttggctggat tacctggcgc 2701 gcctcaaagg ccatttacce caacttgate aacaaggcat ccgctaccgg 2751 gcgccttgga aagcataacg caacacgttt tctctagcat cgacattgag 2801 tggcgccaat gcgccactgt ttaaaaagga aattaccatg aaatacggct 2851 atttcgataa cgacaatcge gaatacgtca ttactcgtce cgatgttcct 2901 gcaccttgga ccaactacct cggcacggaa aaattctgca ccgtcatcte 2951 ccataatgcg gggggctact cgttctatca ctcacccgag tacaaccgtg 3001 tgaccaagtt ccgtccgaac ttcacacaag atcgtcccgg gcattacatc 3051 tatttgcgcg atgatgaaac cggtgatttc tggtcggtct cttggcagcc 3101 cgttgccaaa aaccttgacg atgcccatta cgaagtgcgc catggatgcc 3151 gtgtatgagt atctgttctc cccatacggt ttacacctca acgccccctc 3201 gtttgcaacg cccaacgatg acatcggttt tgtcacccgc gtctaccaag 3251 gcgtgaaaga aaacggtgcg attttctcgc atccgaaccc gtgggcatgg 3301 gtcgccgaag ccaaactggg acgcggtgat cgcgcgatgg aattctacga 3351 ttcgctcaac ccatacaacc agaacgacat cattgaaacg cgcgtggcag 3401 agccatattc ctacgtgcaa ttcatcatgg gtcgcgacca ccaagatcac 3451 ggccgtgcaa accacccttg gctcaccggt acatcgggct gggcctacta 3501 cgcgaccacc aacttcattt tgggagtgcg taccggattt gacaggttga 3551 ccgtggatcc atgtattcct gccgcttggt cgggctttga gcgtcacgcg 3601 cgagtggcgc ggtgcgacgt atcacatgtc agtccaaaac ccgaatggcg 3651 tcagcaaagg cgtgcaatcg 2) INFORMATION FOR SEQ ID NO: 8: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 2000 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: cDNA (vi) ORIGINAL SOURCE: (B) STRAIN: Trichoderma harzianum CBS 243.71 (ix) FEATURE: (A) NAME/KEY: CBS (B) LOCATION;86..1819 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 8: GACATCTCCA CCATAGAGTC GACTCATTGC TGGCATACGG AGCATTCCAA TCTTACTCGT 60 AGTAGTGTTA TTGCCATCGC TCATC ATG CTG CCC AAG GCG ATC ATC GCG ATT 112 Met Leu Pro Lys Ala Ile Ile Ala Ile 1 5 GCC GCA TTG GCT TTC AGC CCA GCA AAT GCG CTG TGG CCC ATT CCT CAG 160 Ala Ala Leu Ala Phe Ser Pro Ala Asn Ala Leu Trp Pro Ile Pro Gln 10 15 20 25 AAG ATC TCG ACC GGA GAC AGC GTG CTC TTT ATT GAC CAG GCT GTT AGG 208 Lys Ile Ser Thr Gly Asp Ser Val Leu Phe Ile Asp Gln Ala Val Arg 30 35 40 GTG ACT TAC AAT GGA GTA CCG ATC ATC CCT ATC GGC TAC AAC CCA CCG 256 Val Thr Tyr Asn Gly Val Pro Ile Ile Pro Ile Gly Tyr Asn Pro Pro 45 50 55 GCC AGC TCC AAC TTC GAC AGC AGG CAA ATC GTC CAG GCG GCT GTC TCG 304 Ala Ser Ser Asn Phe Asp Ser Arg Gln Ile Val Gln Ala Ala Val Ser 60 65 704 CGC GCT TTC CAA AAC ATC TTC AGC ACC AAC TAT GTG CCA TGG AAG CTT 352 Arg Ala Phe Gln Asn Ile Phe Ser Thr Asn Tyr Val Pro Trp Lys Leu 75 80 85 CAC CCG CGT AAC AGC AAC TTT GAG CCG AAG GTG GCC CCT CAG AAC CGA 400 His Pro Arg Asn Ser Asn Phe Glu Pro Lys Val Ala Pro Gln Asn Arg 90 95 100 105 ATC CAG TCC ATC TCA ATT CAG CAG ACT GGA AAG GAT ACG TCC AAG ACG 448 Ile Gln Ser Ile Ser Ile Gln Gln Thr Gly Lys Asp Thr Ser Lys Thr 110 115 120 TTC AAG CCG CGC GCC GGA GAC GTT GAT GAG TCG TAC TCT TTG ACC ATT 496 Phe Lys Pro Arg Ala Gly Asp Val Asp Glu Ser Tyr Ser Leu Thr Ile 125 130 135 TCC AAG AAT GGA CAG GTC AAC ATC AGT GCC AAG TCT TCT ACT GGT GTG 544 Ser Lys Asn Gly Gln Val Asn Ile Ser Ala Lys Ser Ser Thr Gly Val 140 145 150 CTG CAC GCC CTC GAG ACC TTC TCG CAG CTT TTC TAC AAG CAC TCT GCT 592 Leu His Ala Leu Glu Thr Phe Ser Gln Leu Phe Tyr Lys His Ser Ala 155 160 165 GGA CCT TTC TAC TAT ACG ACT CAG GCT CCC GTG TCC ATC ACA GAC GCT 640 Gly Pro Phe Tyr Tyr Thr Thr Gln Ala Pro Val Ser Ile Thr Asp Ala 170 175 180 185 CCC AAA TAT CCC CAC CGT GGC ATC ATG CTT GAC CTT GCC CGT AAC TAT 688 Pro Lys Tyr Pro His Arg Gly Ile Met Leu Asp Leu Ala Arg Asn Tyr 190 195 200 CAA ACC ATT GAT GAC ATC AAG AGG ACC ATT GAC GCC ATG TCG TGG AAC 736 Gln Thr Ile Asp Asp Ile Lys Arg Thr Ile Asp Ala Met Ser Trp Asn 205 210 215 AAG CTT AAC CGC CTG CAC TTG CAC ATC ACC GAC TCT CAG TCG TGG CCG 784 Lys Leu Asn Arg Leu His Leu His Ile Thr Asp Ser Gln Ser Trp Pro 220 225 230 CTG GTG ATC CCC TCG CTG CCT AAG CTG TCC CAG GCC GGT GCC TAC CAC 832 Leu Val Ile Pro Ser Leu Pro Lys Leu Ser Gln Ala Gly Ala Tyr His 235 240 245 CCC AGC CTC GTC TAC ACT CCC GCA GAC CTT GCT GGC ATT TTC CAG TAC 880 Pro Ser Leu Val Tyr Thr Pro Ala Asp Leu Ala Gly Ile Phe Gln Tyr 250 255 260 265 GGT GTC GCC CGC GGT GTT GAG GTC ATT ACG GAG ATC GAT ATG CCT GGC 928 Gly Val Ala Arg Gly Val Glu Val Ile Thr Glu Ile Asp Met Pro Gly 270 275 280 CAC ATC GGT GTT ATC GAG CTC GCT TAC AGC GAT CTC ATT GTT GCC TAC 976 His Ile Gly Val Ile Glu Leu Ala Tyr Ser Asp Leu Ile Val Ala Tyr 285 290 295 GAA GAG ATG CCT TAC CAG TAC TAC TGC GCC GAG CCA CCT TGC GGT GCC 1024 Glu Glu Met Pro Tyr Gln Tyr Tyr Cys Ala Glu Pro Pro Cys Gly Ala 300 305 310 TTT TCC ATC AAC AAC ACC AAG GTG TAC AGC TTC CTC GAT ACC CTG TTC 1072 Phe Ser Ile Asn Asn Thr Lys Val Tyr Ser Phe Leu Asp Thr Leu Phe 315 320 325 GAC GAC CTT TTG CCT CGC GTC GCT CCT TAC AGC GCG TAC TTC CAC ACC 1120 Asp Asp Leu Leu Pro Arg Val Ala Pro Tyr Ser Ala Tyr Phe His Thr 330 335 340 345 GGT GGT GAC GAG CTC AAC GCT AAC GAC TCC ATG CTC GAC TCT CAC ATC 1168 Gly Gly Asp Glu Leu Asn Ala Asn Asp Ser Met Leu Asp Ser His Ile 350 355 360 AAG AGC AAC GAG ACC TCC GTT CTG CAA CCT CTG CTG CAA AAG TTC ATC 1216 Lys Ser Asn Glu Thr Ser Val Leu Gln Pro Leu Leu Gln Lys Phe Ile 365 370 375 AAC TTT GCC CAC TCC AAG GTC CGT GCC GCG GGC TTG TCG CCA TTT GTC 1264 Asn Phe Ala His Ser Lys Val Arg Ala Ala Gly Leu Ser Pro Phe Val 380 385 390 TGG GAG GAG ATG GTC ACC ACC TGG AAC CTG ACC CTC GGC AGC GAC ACC 1312 Trp Glu Glu Met Val Thr Thr Trp Asn Leu Thr Leu Gly Ser Asp Thr 395 400 405 GTC GTT CAG TCG TGG CTG GGT GGC GAT GCC GTC AAG AAC CTG GCT GAG 1360 Val Val Gln Ser Trp Leu Gly Gly Asp Ala Val Lys Asn Leu Ala Glu 410 415 420 425 AGC GGC CAC AAG GTC ATT GAC ACC GAC TAC AAC TTC TAC TAC TTG GAC 1408 Ser Gly His Lys Val Ile Asp Thr Asp Tyr Asn Phe Tyr Tyr Leu Asp 430 435 440 TGC GGC CGT GGT CAA TGG GTC AAC TTC CCT CCA GGA GAC TCC TAC AAC 1456 Cys Gly Arg Gly Gln Trp Val Asn Phe Pro Pro Gly Asp Ser Tyr Asn 445 450 455 ACC TAC TAC CCA TTC AAC GAC TGG TGC CAG CCC ACC AAG AAC TGG AGG 1504 Thr Tyr Tyr Pro Phe Asn Asp Trp Cys Gln Pro Thr Lys Asn Trp Arg 460 465 470 CTC ATC TAC TCT CAC GAC CCT GCA GCC AAC GTG TCT GCT TCG GCT GCC 1552 Leu Ile Tyr Ser His Asp Pro Ala Ala Asn Val Ser Ala Ser Ala Ala 475 480 485 AAG AAC GTC CTG GGA GGA GAG CTT GCC ATT TGG AGC GAG ATG ATT GAC 1600 Lys Asn Val Leu Gly Gly Glu Leu Ala Ile Trp Ser Glu Met Ile Asp 490 495 500 505 GCC AGC AAC CTG GAC AAC ATC ATC TGG CCT CGT GGC AGC GCC GCC GGT 1648 Ala Ser Asn Leu Asp Asn Ile Ile Trp Pro Arg Gly Ser Ala Ala Gly 510 515 520 GAG GTT TGG TGG TCC GGC AAT ACC GAT GCC TCT GGT GAG CAG CGC AGC 1696 Glu Val Trp Trp Ser Gly Asn Thr Asp Ala Ser Gly Glu Gln Arg Ser 525 530 535 CAG CTG GAC GTT GTT CCT CGT CTG AAC GAG TTC CGA GAA CGC TTG CTT 1744 Gln Leu Asp Val Val Pro Arg Leu Asn Glu Phe Arg Glu Arg Leu Leu 540 545 550 GCT CGT GGT GTC AGC GCG TTC CCC ATC CAG ATG ACC TAC TGC ACT CAG 1792 Ala Arg Gly Val Ser Ala Phe Pro Ile Gln Met Thr Tyr Cys Thr Gln 555 560 565 CTC AAC GCC ACT GCC TGC ACA CTA TTT TAAGTCTAAG ATGACTTTTT 1839 Leu Asn Ala Thr Ala Cys Thr Leu Phe 570 575 CTTTTATTGG GCAGGGTTTT TTCTATTTTT CACGTATTAT CATTAGTGTA CAGTGATTAA 1899 AACAGGTATG GCTTAAGAGG AGCTGGGAGG GTATCCGGCT TGGGGCGGTA TATTATTAAC 1959 TGTATATAAT TCAAATTCAT CTACATATAT GTTATGAAAA A 2000 2) INFORMATION FOR SEQ ID NO: 9: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 2239 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: cDNA (vi) ORIGINAL SOURCE: (B) STRAIN: Trichoderma harzianum CBS 243.71 (ix) FEATURE: (A) NAME/KEY: CDS (B) LOCATION; 282..2086 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 9: CTGAGAAGCG GCACTTGCTG ATCTGCGTGG AACTTGGGGT TACAACGCAC CGGATAGCTC 60 ATCTCCCCAG GACCCCGGAA CTGGAGCTGG AACTGGAATT GGAGCTGGAG CGGACCCAGG 120 CCGGAGACGA GAAACACAGT GAATCACTCC TGCAAGGGGC GGGACAGGAA CGTGGACAGT 180 ATTTAGTTTA AGCAGCTGTC CCAGAGCTGT TCGCCCTGCT TCCAAGCTCG TGTGGCCTGA 240 CCCTGTATAA ACTCATTACG ACCATCAGCT CACAGCCGAC A ATG TTT TCC AGG 293 Met Phe Ser Arg 1 GCC ATT GTC GCC GCA TTG GCC CTG AGC GGC CCG GCT TTT GCC CTG TGG 341 Ala Ile Val Ala Ala Leu Ala Leu Ser Gly Pro Ala Phe Ala Leu Trp 5 10 15 20 CCC GTG CCT AAA CAC TCC TCG ACC GGC AAT GAC ACG CTC TTT ATT GAC 389 Pro Val Pro Lys His Ser Ser Thr Gly Asn Asp Thr Leu Phe Ile Asp 25 30 35 CAG ACG GTC CAG GTT ACC TAC AAT GGT GAA CAG GTG TGG TGG ACT CCT 437 Gln Thr Val Gln Val Thr Tyr Asn Gly Glu Gln Val Trp Trp Thr Pro 40 45 50 CCA TAT GAT GAC CCC GGA TCC CCG GAC TTT GCT GAG ACC AGG ATC GAT 485 Pro Tyr Asp Asp Pro Gly Ser Pro Asp Phe Ala Glu Thr Arg Ile Asp 55 60 65 GAC CAA CAG GTT ACT TAC ACG GCC GGC TAC GTG CCT CCC AGC GGA CCG 533 Asp Gln Gln Val Thr Tyr Thr Ala Gly Tyr Val Pro Pro Ser Gly Pro 70 75 80 CAT TTC ACC AGC AAG GAA ATC GTT CAA GGC GGC GTC TCG CGG ACA TTC 581 His Phe Thr Ser Lys Glu Ile Val Gln Gly Gly Val Ser Arg Thr Phe 85 90 95 100 GGC GCC ATC TTC CAG CAG GGC TTT GTG CCG TGG ATG CTG CGT GAA CGT 629 Gly Ala Ile Phe Gln Gln Gly Phe Val Pro Trp Met Leu Arg Glu Arg 105 110 115 GAT TCG AAC TCT GAA CCG AAT CTA GGC GGA ACG CGG ATC CGG ACA CTG 677 Asp Ser Asn Ser Glu Pro Asn Leu Gly Gly Thr Arg Ile Arg Thr Leu 120 125 130 CAG ATT ATA CAG ACT CAG CAC GAT TCT GCG AAT ACC TTC AAG CCT CTG 725 Gln Ile Ile Gln Thr Gln His Asp Ser Ala Asn Thr Phe Lys Pro Leu 135 140 145 AAT GGC GCA GTG AAT GAA TCC TAT GCC CTG GAT GTC GAC GCA AAG GGC 773 Asn Gly Ala Val Asn Glu Ser Tyr Ala Leu Asp Val Asp Ala Lys Gly 150 155 160 CAC GCA TCT CTC ACC GCT CCG TCG TCA ACG GGC ATC CTT CGA GGC CTT 821 His Ala Ser Leu Thr Ala Pro Ser Ser Thr Gly Ile Leu Arg Gly Leu 165 170 175 180 GAG ACC TTC TCC CAG CTC TTC TTC AAG CAT AGC TCC GGC ACT GCT TGG 869 Glu Thr Phe Ser Gln Leu Phe Phe Lys His Ser Ser Gly Thr Ala Trp 185 190 195 TAT ACG CAG CTT GCA CCT GTT TCG ATC CGC GAT GAG CCC AAG TAT CCT 917 Tyr Thr Gln Leu Ala Pro Val Ser Ile Arg Asp Glu Pro Lys Tyr Pro 200 205 210 CAC CGC GGC CTC CTG TTG GAT GTC AGC CGC CAT TGG TTC GAG GTT TCC 965 His Arg Gly Leu Leu Leu Asp Val Ser Arg His Trp Phe Glu Val Ser 215 220 225 GAC ATT GAG CGC ACT ATC GAT GCT CTG GCC ATG AAC AAA ATG AAT GTG 1013 Asp Ile Glu Arg Thr Ile Asp Ala Leu Ala Met Asn Lys Met Asn Val 230 235 240 CTG CAT CTG CAC GCT ACT GAC ACG CAG TCA TGG CCG CTG GAG ATT CCA 1061 Leu His Leu His Ala Thr Asp Thr Gln Ser Trp Pro Leu Glu Ile Pro 245 250 255 260 TCC CTG CCT CTG CTG GCT GAG AAG GGC GCC TAT CAC AAG GGT TTG AGC 1109 Ser Leu Pro Leu Leu Ala Glu Lys Gly Ala Tyr His Lys Gly Leu Ser 265 270 275 TAC TCG CCA AGC GAT CTT GCG AGC ATC CAA GAA TAT GGT GTT CAT CGA 1157 Tyr Ser Pro Ser Asp Leu Ala Ser Ile Gln Glu Tyr Gly Val His Arg 280 285 290 GGT GTC CAG GTC ATT GTA GAG ATT GAT ATG CCG GGC CAC GTT GGA ATC 1205 Gly Val Gln Val Ile Val Glu Ile Asp Met Pro Gly His Val Gly Ile 295 300 305 GAC AAG GCA TAC CCC GGG CTT AGC AAC GCC TAC GGA GTC AAC CCG TGG 1253 Asp Lys Ala Tyr Pro Gly Leu Ser Asn Ala Tyr Gly Val Asn Pro Trp 310 315 320 CAG TGG TAC TGC GCC CAG CCG CCC TGC GGA TCT TTC AAG CTG AAC AAC 1301 Gln Trp Tyr Cys Ala Gln Pro Pro Cys Gly Ser Phe Lys Leu Asn Asn 325 330 335 340 ACG GAT GTC GAA AAG TTC ATT GAC AAG CTG TTT GAA GAT TTG CTG CCG 1349 Thr Asp Val Glu Lys Phe Ile Asp Lys Leu Phe Glu Asp Leu Leu Pro 345 350 355 CGT CTT TCG CCG TAC TCG GCC TAC TTT CAC ACT GGT GGC GAT GAG TAC 1397 Arg Leu Ser Pro Tyr Ser Ala Tyr Phe His Thr Gly Gly Asp Glu Tyr 360 365 370 AAG GCG AAC AAC TCG CTG CTC GAC CCG GCC CTT CGC ACA AAC GAC ATG 1445 Lys Ala Asn Asn Ser Leu Leu Asp Pro Ala Leu Arg Thr Asn Asp Met 375 380 385 AAC ACC CTG CAG CCG ATG CTG CAG CGC TTC TTG GAC CAC GTG CAT GGC 1493 Asn Thr Leu Gln Pro Met Leu Gln Arg Phe Leu Asp His Val His Gly 390 395 400 AAA GTT CGT GAT CTG GGA CTC GTT CCC ATG GTT TGG GAA GAA ATG ATT 1541 Lys Val Arg Asp Leu Gly Leu Val Pro Met Val Trp Glu Glu Met Ile 405 410 415 420 CTG GAT TGG AAC GCA ACT CTG GGC AAG GAT GTC GTT GCT CAA ACG TGG 1589 Leu Asp Trp Asn Ala Thr Leu Gly Lys Asp Val Val Ala Gln Thr Trp 425 430 435 CTT GGC GGA GGA GCG ATT CAG AAG CTT GCT CAG GCT GGA TAC AAG GTT 1637 Leu Gly Gly Gly Ala Ile Gln Lys Leu Ala Gln Ala Gly Tyr Lys Val 440 445 450 ATT GAC AGC AGC AAT GAC TTT TAC TAT CTC GAC TGT GGT CGT GGT GAG 1685 Ile Asp Ser Ser Asn Asp Phe Tyr Tyr Leu Asp Cys Gly Arg Gly Glu 455 460 465 TGG CTC GAT TTT GCC AAT GGT GAC CCC TTT AAC AAC AAC TAT CCC TTT 1733 Trp Leu Asp Phe Ala Asn Gly Asp Pro Phe Asn Asn Asn Tyr Pro Phe 470 475 480 CTC GAC TGG TGC GAC CCG ACC AAA AAC TGG AAG CTC ATG TAC TCA CAC 1781 Leu Asp Trp Cys Asp Pro Thr Lys Asn Trp Lys Leu Met Tyr Ser His 485 490 495 500 GAG CCC ACG GAC GGC GTG TCC GAT GAT CTC AAG AAG AAT GTC ATT GGA 1829 Glu Pro Thr Asp Gly Val Ser Asp Asp Leu Lys Lys Asn Val Ile Gly 505 510 515 GGC GAA GTT GCT GTC TGG ACT GAG ACC ATC GAT CCG ACC AGC TTG GAC 1877 Gly Glu Val Ala Val Trp Thr Glu Thr Ile Asp Pro Thr Ser Leu Asp 520 525 530 TCC ATC ATC TGG CCG CGA GCG GGA GCG GCC GCT GAG ATT TGG TGG TCG 1925 Ser Ile Ile Trp Pro Arg Ala Gly Ala Ala Ala Glu Ile Trp Trp Ser 535 540 545 GGC AAG ATC GAT GAG AAG GGC CAG AAC CGA TCA CAG ATT GAT GCA CGG 1973 Gly Lys Ile Asp Glu Lys Gly Gln Asn Arg Ser Gln Ile Asp Ala Arg 550 555 560 CCA AGA TTA TCG GAG CAG CGA GAG CGC ATG TTG GCG AGG GGA GTT CGA 2021 Pro Arg Leu Ser Glu Gln Arg Glu Arg Met Leu Ala Arg Gly Val Arg 565 570 575 580 GGA ACG CCG ATT ACG CAG CTG TGG TGT AGT CAG GTT GAT GTT CAT AAC 2069 Gly Thr Pro Ile Thr Gln Leu Trp Cys Ser Gl Val Asp Val His Asn 585 590 595 TGC GAG TCT GGG AAT TA CTGATGCGGG TTGATGAACA AAGTATGTAA 2116 Cys Glu Ser Gly Asn 600 <BR> <BR> <BR> <BR> <BR> <BR> <BR> <BR> TGTGGTATAT ATGAATGTTT CTTTTTCACG CTGCTGTTAA AGGCCGGGGA CGTCTCGTT 2176 GTGATGACGG TTAGACTGAA AATCACTTAT AATGAATTCA AGTCATTCAA GATGAAAAAA 2236 AAA 2239 2) INFORMATION FOR SEQ ID NO: 10: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 578 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 10: Met Leu Pro Lys Ala Ile Ile Ala Ile Ala Ala Leu Ala Phe Ser Pro 1 5 10 15 Ala Asn Ala Leu Trp Pro Ile Pro Gln Lys Ile Ser Thr Gly Asp Ser 20 25 30 Val Leu Phe Ile Asp Gln Ala Val Arg Val Thr Tyr Asn Gly Val Pro 35 40 45 Ile Ile Pro Ile Gly Tyr Asn Pro Pro Ala Ser Ser Asn Phe Asp Ser 50 55 60 Arg Gln Ile Val Gln Ala Ala Val Ser Arg Ala Phe Gln Asn Ile Phe 65 70 75 80 Ser Thr Asn Tyr Val Pro Trp Lys Leu His Pro Arg Asn Ser Asn Phe 85 90 95 Glu Pro Lys Val Ala Pro Gln Asn Arg Ile Gln Ser Ile Ser Ile Gln 100 105 110 Gln Thr Gly Lys Asp Thr Ser Lys Thr Phe Lys Pro Arg Ala Gly Asp 115 120 125 Val Asp Glu Ser Tyr Ser Leu Thr Ile Ser Lys Asn Gly Gln Val Asn 130 135 140 Ile Ser Ala Lys Ser Ser Thr Gly Val Leu His Ala Leu Glu Thr Phe 145 150 155 160 Ser Gln Leu Phe Tyr Lys His Ser Ala Gly Pro Phe Tyr Tyr Thr Thr 165 170 175 Gln Ala Pro Val Ser Ile Thr Asp Ala Pro Lys Tyr Pro His Arg Gly 180 185 190 Ile Met Leu Asp Leu Ala Arg Asn Tyr Gln Thr Ile Asp Asp Ile Lys 195 200 205 Arg Thr Ile Asp Ala Met Ser Trp As Lys Leu Asn Arg Leu His Leu 210 215 220 His Ile Thr Asp Ser Gln Ser Trp Pro Leu Val Ile Pro Ser Leu Pro 225 230 235 240 Lys Leu Ser Gln Ala Gly Ala Tyr His Pro Ser Leu Val Tyr Thr Pro 245 250 255 Ala Asp Leu Ala Gly Ile Phe Gln Tyr Gly Val Ala Arg Gly Val Glu 260 265 270 Val Ile Thr Glu Ile Asp Met Pro Gly His Ile Gly Val Ile Glu Leu 275 280 285 Ala Tyr Ser Asp Leu Ile Val Ala Tyr Glu Glu Met Pro Tyr Gln Tyr 290 295 300 Tyr Cys Ala Glu Pro Pro Cys Gly Ala Phe Ser Ile Asn Asn Thr Lys 305 310 315 320 Val Tyr Ser Phe Leu Asp Thr Leu Phe Asp Asp Leu Leu Pro Arg Val 325 330 335 Ala Pro Tyr Ser Ala Tyr Phe His Thr Gly Gly Asp Glu Leu Asn Ala 340 345 350 Asn Asp Ser Met Leu Asp Ser His Ile Lys Ser Asn Glu Thr Ser Val 355 360 365 Leu Gln Pro Leu Leu Gln Lys Phe Ile Asn Phe Ala His Ser Lys Val 370 375 380 Arg Ala Ala Gly Leu Ser Pro Phe Val Trp Glu Glu Met Val Thr Thr 385 390 395 400 Trp Asn Leu Thr Leu Gly Ser Asp Thr Val Val Gln Ser Trp Leu Gly 405 410 415 Gly Asp Ala Val Lys Asn Leu Ala Glu Ser Gly His Lys Val Ile Asp 420 425 430 Thr Asp Tyr Asn Phe Tyr Tyr Leu Asp Cys Gly Arg Gly Gln Trp Val 435 440 445 Asn Phe Pro Pro Gly Asp Ser Tyr Asn Thr Tyr Tyr Pro Phe Asn Asp 450 455 460 Trp Cys Gln Pro Thr Lys Asn Trp Arg Leu Ile Tyr Ser His Asp Pro 465 470 475 480 Ala Ala Asn Val Ser Ala Ser Ala Ala Lys Asn Val Leu Gly Gly Glu 485 490 495 Leu Ala Ile Trp Ser Glu Met Ile Asp Ala Ser Asn Leu Asp Asn Ile 500 505 510 Ile Trp Pro Arg Gly Ser Ala Ala Gly Glu Val Trp Trp Ser Gly Asn 515 520 525 Thr Asp Ala Ser Gly Glu Gln Arg Ser Gln Leu Asp Val Val Pro Arg 530 535 540 Leu Asn Glu Phe Arg Glu Arg Leu Leu Ala Arg Gly Val Ser Ala Phe 545 550 555 560 Pro Ile Gln Met Thr Tyr Cys Thr Gln Leu Asn Ala Thr Ala Cys Thr 565 570 575 Leu Phe 2) INFORMATION FOR SEQ ID NO: ii: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 601 base pairs (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 11: Met Phe Ser Arg Ala Ile Val Ala Ala Leu Ala Leu Ser Gly Pro Ala 1 5 10 15 Phe Ala Leu Trp Pro Val Pro Lys His Ser Ser Thr Gly Asn Asp Thr 20 25 30 Leu Phe Ile Asp Gln Thr Val Gln Val Thr Tyr Asn Gly Glu Gln Val 35 40 45 Trp Trp Thr Pro Pro Tyr Asp Asp Pro Gly Ser Pro Asp Phe Ala Glu 50 55 60 Thr Arg Ile Asp Asp Gln Gln Val Thr Tyr Thr Ala Gly Tyr Val Pro 65 70 75 80 Pro Ser Gly Pro His Phe Thr Ser Lys Glu Ile Val Gln Gly Gly Val 85 90 95 Ser Arg Thr Phe Gly Ala Ile Phe Gln Gln Gly Phe Val Pro Trp Met 100 105 110 Leu Arg Glu Arg Asp Ser Asn Ser Glu Pro Asn Leu Gly Gly Thr Arg 115 120 125 Ile Arg Thr Leu Gln Ile Ile Gln Thr Gln His Asp Ser Ala Asn Thr 130 135 140 Phe Lys Pro Leu Asn Gly Ala Val Asn Glu Ser Tyr Ala Leu Asp Val 145 150 155 160 Asp Ala Lys Gly His Ala Ser Leu Thr Ala Pro Ser Ser Thr Gly Ile 165 170 175 Leu Arg Gly Leu Glu Thr Phe Ser Gln Leu Phe Phe Lys His Ser Ser 180 185 190 Gly Thr Ala Trp Tyr Thr Gln Leu Ala Pro Val Ser Ile Arg Asp Glu 195 200 205 Pro Lys Tyr Pro His Arg Gly Leu Leu Leu Asp Val Ser Arg His Trp 210 215 220 Phe Glu Val Ser Asp Ile Glu Arg Thr Ile Asp Ala Leu Ala Met Asn 225 230 235 240 Lys Met Asn Val Leu His Leu His Ala Thr Asp Thr Gln Ser Trp Pro 245 250 255 Leu Glu Ile Pro Ser Leu Pro Leu Leu Ala Glu Lys Gly Ala Tyr His 260 265 270 Lys Gly Leu Ser Tyr Ser Pro Ser Asp Leu Ala Ser Ile Gln Glu Tyr 275 280 285 Gly Val His Arg Gly Val Gln Val Ile Val Glu Ile Asp Met Pro Gly 290 295 300 His Val Gly Ile Asp Lys Ala Tyr Pro Gly Leu Ser Asn Ala Tyr Gly 305 310 315 320 Val Asn Pro Trp Gln Trp Tyr Cys Ala Gln Pro Pro Cys Gly Ser Phe 325 330 335 Lys Leu Asn Asn Thr Asp Val Glu Lys Phe Ile Asp Lys Leu Phe Glu 340 345 350 Asp Leu Leu Pro Arg Leu Ser Pro Tyr Ser Ala Tyr Phe His Thr Gly 355 360 365 Gly Asp Glu Tyr Lys Ala Asn Asn Ser Leu Leu Asp Pro Ala Leu Arg 370 375 380 Thr Asn Asp Met Asn Thr Leu Gln Pro Met Leu Gln Arg Phe Leu Asp 385 390 395 400 His Val His Gly Lys Val Arg Asp Leu Gly Leu Val Pro Met Val Trp 405 410 415 Glu Glu Met Ile Leu Asp Trp Asn Ala Thr Leu Gly Lys Asp Val Val 420 425 430 Ala Gln Thr Trp Leu Gly Gly Gly Ala Ile Gln Lys Leu Ala Gln Ala 435 440 445 Gly Tyr Lys Val Ile Asp Ser Ser Asn Asp Phe Tyr Tyr Leu Asp Cys 450 455 460 Gly Arg Gly Glu Trp Leu Asp Phe Ala Asn Gly Asp Pro Phe Asn Asn 465 470 475 480 Asn Tyr Pro Phe Leu Asp Trp Cys Asp Pro Thr Lys Asn Trp Lys Leu 485 490 495 Met Tyr Ser His Glu Pro Thr Asp Gly Val Ser Asp Asp Leu Lys Lys 500 505 510 Asn Val Ile Gly Gly Glu Val Ala Val Trp Thr Glu Thr Ile Asp Pro 515 520 525 Thr Ser Leu Asp Ser Ile Ile Trp Pro Arg Ala Gly Ala Ala Ala Glu 530 535 540 Ile Trp Trp Ser Gly Lys Ile Asp Glu Lys Gly Gln As Arg Ser Gln 545 550 555 560 Ile Asp Ala Arg Pro Arg Leu Ser Glu Gln Arg Glu Arg Met Leu Ala 565 570 575 Arg Gly Val Arg Gly Thr Pro Ile Thr Gln Leu Trp Cys Ser Gln Val 580 585 590 Asp Val His As Cys Glu Ser Gly Asn 595 600