Title:
LENGTH DETERMINATION OF NUCLEIC ACID REPEAT SEQUENCES BY DISCONTINUOUS PRIMER EXTENSION
Document Type and Number:
WIPO Patent Application WO2000032824
Kind Code:
A3
Abstract:
Disclosed is a method for determining the number of repeat units in a repeat region of a target nucleic acid. The method comprises a discontinuous primer extension reaction wherein a primer is extended in discrete increments corresponding to a single repeat unit. Following each increment of primer extension, a detection step is performed in which a modulation in a signal is detected when the primer has been extended by an amount equal to the total length of a repeat region. By counting the number of increments of discrete primer extension required to cause a modulation in the signal, the number of repeat units making up the repeat region is determined. In the method, a plurality of different-sequence primers are contacted with a polynucleotide sample under conditions effective for the primers to anneal to primer-complementary regions in one or more target polynucleotides, to form one or more target-primer hybrid(s), wherein either (1) each different-sequence primer contains (i) a target binding segment and (ii) a tag segment having a nucleotide sequence that uniquely identifies the target binding segment, or (2) one or more polynucleotides in the sample are tagged polynucleotides that contain a tag segment having a nucleotide sequence that uniquely identifies the attached polynucleotide. The tagged moieties can conveniently be immobilized on an array of immobilized tag complements for analysis.
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Inventors:
LIVAK KENNETH J
LOWE ADAM L
BLASBAND ANDREW J
LOWE ADAM L
BLASBAND ANDREW J
Application Number:
PCT/US1999/028584
Publication Date:
May 10, 2001
Filing Date:
December 01, 1999
Export Citation:
Assignee:
PERKIN ELMER CORP (US)
International Classes:
C12Q1/68; C12N15/09; C12Q1/6827; C12Q1/6837; C12Q1/6858; C12Q1/6874; G01N33/53; G01N33/566; (IPC1-7): C12Q1/68
Domestic Patent References:
| WO1996041011A1 | 1996-12-19 | |||
| WO1996036737A1 | 1996-11-21 | |||
| WO1998054362A1 | 1998-12-03 |
Foreign References:
| US5650277A | 1997-07-22 | |||
| FR2718753A1 | 1995-10-20 | |||
| DE4141178A1 | 1993-06-17 |
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