Title:
LIMONIN EXTRACTION METHOD
Document Type and Number:
WIPO Patent Application WO/2015/188611
Kind Code:
A1
Abstract:
A limonin extraction method, comprising the following steps: step one, raw material digesting or juicing: directly soaking raw material in water or using a presser to directly juice the raw material to obtain the extract; step two, adding salt and adjusting pH: adding a sulfate solid material or sulfate saturated solution to the extract; adjusting pH to 3-7; step three, heating and preserving heat: heating to 20°C -100°C and preserving heat for 10 min -110 min; step four, centrifuging: centrifuging for 10 min at a rotational speed of 2500×g, and obtaining limonin precipitation. Compared with a traditional method, the raw material in the present invention can directly use the extract liquid or juicing liquid without drying, and recover most limonin in water using a precipitation method without complicated apparatus, and is easy to operate and has a low process cost.
Inventors:
NI HUI (CN)
CAI HUINONG (CN)
YANG YUANFAN (CN)
GAO QIUFEN (CN)
CHEN FENG (CN)
DU XIPING (CN)
HUANG GAOLING (CN)
WU LING (CN)
XIAO ANFENG (CN)
CAI HUINONG (CN)
YANG YUANFAN (CN)
GAO QIUFEN (CN)
CHEN FENG (CN)
DU XIPING (CN)
HUANG GAOLING (CN)
WU LING (CN)
XIAO ANFENG (CN)
Application Number:
PCT/CN2015/000299
Publication Date:
December 17, 2015
Filing Date:
April 30, 2015
Export Citation:
Assignee:
UNIV JIMEI (CN)
International Classes:
C07J73/00
Foreign References:
| CN101897452A | 2010-12-01 | |||
| US4305923A | 1981-12-15 | |||
| CN104004051A | 2014-08-27 |
Other References:
WANG, JIANHONG ET AL.: "Study on extraction of limonin from lemon pulp by aqueous two-phase system of the ethanol-(NH4)2SO4", SCIENCE AND TECHNOLOGY OF FOOD INDUSTRY, vol. 35, no. 3, 22 September 2013 (2013-09-22), pages 230 - 232
GAO, YIYONG: "Research on extraction and industrialization of limonoids in Citrus Peng", SCIENCE AND TECHNOLOGY OF FOOD INDUSTRY, vol. 30, no. 7, 31 December 2009 (2009-12-31), pages 359 - 362
HIROSHI IWATA ET AL.: "Mechanism-Based Inactivation of Human Liver Microsomal CYP3A4 by Rutaecarpine and Limonin from Evodia Fruit Extract", DRUG METAB. PHARMACOKINET, vol. 20, no. 1, 31 December 2005 (2005-12-31), pages 34 - 45, XP055243122
GAO, YIYONG: "Research on extraction and industrialization of limonoids in Citrus Peng", SCIENCE AND TECHNOLOGY OF FOOD INDUSTRY, vol. 30, no. 7, 31 December 2009 (2009-12-31), pages 359 - 362
HIROSHI IWATA ET AL.: "Mechanism-Based Inactivation of Human Liver Microsomal CYP3A4 by Rutaecarpine and Limonin from Evodia Fruit Extract", DRUG METAB. PHARMACOKINET, vol. 20, no. 1, 31 December 2005 (2005-12-31), pages 34 - 45, XP055243122
Download PDF:
Previous Patent: METHOD AND DEVICE FOR ESTIMATING STATE OF CHARGE OF BATTERY
Next Patent: CHIMNEY DISASSEMBLING ELEVATOR
Next Patent: CHIMNEY DISASSEMBLING ELEVATOR