Title:
LIPASE MUTANT AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2022/151568
Kind Code:
A1
Abstract:
Provided is a lipase mutant and an application thereof. The amino acid sequence of the lipase mutant has the sequence shown in SEQ ID NO: 1, that is, the amino acid mutation site comprises a V154L site. The protein structure and function of the lipase mutant are changed, the solubility of the expression of same in E. coli is improved, and the catalytic activity of the enzyme is improved. In practical applications, the dosage of the enzyme is reduced, the reaction volume is reduced, and the difficulty of post-processing is reduced, and same is suitable for industrial production.
Inventors:
HONG HAO (US)
JAMES GAGE (US)
XIAO YI (CN)
ZHANG NA (CN)
JIAO XUECHENG (CN)
JIA RU (CN)
LIU WENJING (CN)
LIU YE (CN)
JAMES GAGE (US)
XIAO YI (CN)
ZHANG NA (CN)
JIAO XUECHENG (CN)
JIA RU (CN)
LIU WENJING (CN)
LIU YE (CN)
Application Number:
PCT/CN2021/078741
Publication Date:
July 21, 2022
Filing Date:
March 02, 2021
Export Citation:
Assignee:
ASYMCHEM LIFE SCIENCE TIANJIN CO LTD (CN)
International Classes:
C12N9/20; C12N15/55; C12P7/40
Foreign References:
| CN112375751A | 2021-02-19 | |||
| CN103773746A | 2014-05-07 | |||
| CN109468301A | 2019-03-15 | |||
| CN103881992A | 2014-06-25 | |||
| CN108642025A | 2018-10-12 | |||
| CN108642025A | 2018-10-12 | |||
| CN109750013A | 2019-05-14 |
Other References:
LAN DONG-MING, YANG NING, WANG WEN-KAI, SHEN YAN-FEI, YANG BO, WANG YONG-HUA: "A Novel Cold-Active Lipase from Candida albicans: Cloning, Expression and Characterization of the Recombinant Enzyme", INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 12, no. 6, 1 January 2011 (2011-01-01), pages 3950 - 3965, XP055951959, DOI: 10.3390/ijms12063950
HAN ZHU-PING,YE PENG,WANG XIN-PING: "Lipase Immobilization and Research Progress in Chiral Resolution", APPLIED CHEMICAL INDUSTRY, vol. 40, no. 10, 28 October 2011 (2011-10-28), pages 1823 - 1827+1852, XP055951962, ISSN: 1671-3206, DOI: 10.16581/j.cnki.issn1671-3206.2011.10.038
"Lipase immobilization and research progress in chiral resolution", APPLIED CHEMICAL INDUSTRY, vol. 40, no. 10, 2011, pages 1823 - 1827
"Application of Biocatalysis in the Synthesis of Enantiopure Drugs", AMINO ACIDS & BIOTIC RESOURCES, vol. 35, no. 4, 2013, pages 39 - 42
HAN ZHU-PING,YE PENG,WANG XIN-PING: "Lipase Immobilization and Research Progress in Chiral Resolution", APPLIED CHEMICAL INDUSTRY, vol. 40, no. 10, 28 October 2011 (2011-10-28), pages 1823 - 1827+1852, XP055951962, ISSN: 1671-3206, DOI: 10.16581/j.cnki.issn1671-3206.2011.10.038
"Lipase immobilization and research progress in chiral resolution", APPLIED CHEMICAL INDUSTRY, vol. 40, no. 10, 2011, pages 1823 - 1827
"Application of Biocatalysis in the Synthesis of Enantiopure Drugs", AMINO ACIDS & BIOTIC RESOURCES, vol. 35, no. 4, 2013, pages 39 - 42
Attorney, Agent or Firm:
KANGXIN PARTNERS, P.C. (CN)
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