Title:
LIPID UPTAKE ASSAYS
Document Type and Number:
WIPO Patent Application WO2001088533
Kind Code:
A3
Abstract:
Use of microscopic nematodes such as C. elegans in functional high throughput in vivo assays suitable for the detection of inhibitors or activators of intestinal lipid uptake. Compounds identified as modulators of intestinal lipid uptake using the assays may provide lead compounds for the development of pharmaceutical agents useful in the treatment of diseases of the human or animal (e.g. mammal) body, and in particular diseases and disorders of human and/or animal metabolism, fat handling and/or fat storage, such as obesity, impaired fat metabolism and other related diseases such as diabetes type II and cardiovascular diseases.
Inventors:
VERWAERDE PHILIPPE (FR)
ANTHONISSEN CINDY (BE)
DEPREZ BENOIT (FR)
BONNET BEATRICE (FR)
BOGAERT THIERRY (BE)
ANTHONISSEN CINDY (BE)
DEPREZ BENOIT (FR)
BONNET BEATRICE (FR)
BOGAERT THIERRY (BE)
Application Number:
PCT/IB2001/001061
Publication Date:
May 16, 2002
Filing Date:
May 21, 2001
Export Citation:
Assignee:
DEVGEN NV (BE)
VERWAERDE PHILIPPE (FR)
ANTHONISSEN CINDY (BE)
DEPREZ BENOIT (FR)
BONNET BEATRICE (FR)
BOGAERT THIERRY (BE)
VERWAERDE PHILIPPE (FR)
ANTHONISSEN CINDY (BE)
DEPREZ BENOIT (FR)
BONNET BEATRICE (FR)
BOGAERT THIERRY (BE)
International Classes:
A61K45/00; A61P3/00; A61P3/04; A61P3/06; A61P3/10; A61P9/00; C07F5/02; C07K1/14; C07K14/435; C12N15/01; C12Q1/68; G01N21/77; G01N21/78; G01N33/15; G01N33/50; G01N33/92; (IPC1-7): G01N33/50; G01N33/92; C07F5/02
Foreign References:
| US5338854A | 1994-08-16 |
Other References:
GORDON R ET AL: "UPTAKE OF LIPIDS BY THE ENTOMOPHILIC NEMATODE ROMANOMERMIS-CULICIVORAX", JOURNAL OF NEMATOLOGY, vol. 14, no. 4, 1982, pages 492 - 495, XP001051951, ISSN: 0022-300X
HENDRICKSON H STEWART ET AL: "Intramolecularly quenched BODIPY-labeled phospholipid analogs in phospholipase A2 and platelet-activating factor acetylhydrolase assays and in vivo fluorescence imaging.", ANALYTICAL BIOCHEMISTRY, vol. 276, no. 1, 1 December 1999 (1999-12-01), pages 27 - 35, XP002186412, ISSN: 0003-2697
WITTMANN, VALENTIN; DATTA, ARUN K.; KOELLER, KATHRYN M.; WONG, CHI-HUEY: "Chemoenzymatic Synthesis and Fluorescent Visualization of Cell-Surface Selectin-Bound Sialyl Lewis X Derivatives", CHEMISTRY, A EUROPEAN JOURNAL, vol. 6, no. 1, 2000, pages 162 - 171, XP002186413
RAND J B ET AL: "GENETIC PHARMACOLOGY: INTERACTIONS BETWEEN DRUGS AND GENE PRODUCTS IN CAENORHABDITIS ELEGANS", METHODS IN CELL BIOLOGY, LONDON, GB, vol. 84, 1995, pages 187 - 204, XP000956211
BIRD A F: "A METHOD OF DISTINGUISHING BETWEEN LIVING AND DEAD NEMATODES BY ENZYMATICALLY INDUCED FLUORESCENCE", JOURNAL OF NEMATOLOGY, vol. 11, no. 1, 1979, EN, pages 103 - 105, XP001051693, ISSN: 0022-300X
MATYASH VITALI ET AL: "Distribution and transport of cholesterol in Caenorhabditis elegans.", MOLECULAR BIOLOGY OF THE CELL, vol. 12, no. 6, June 2001 (2001-06-01), pages 1725 - 1736, XP001048139, ISSN: 1059-1524
HENDRICKSON H STEWART ET AL: "Intramolecularly quenched BODIPY-labeled phospholipid analogs in phospholipase A2 and platelet-activating factor acetylhydrolase assays and in vivo fluorescence imaging.", ANALYTICAL BIOCHEMISTRY, vol. 276, no. 1, 1 December 1999 (1999-12-01), pages 27 - 35, XP002186412, ISSN: 0003-2697
WITTMANN, VALENTIN; DATTA, ARUN K.; KOELLER, KATHRYN M.; WONG, CHI-HUEY: "Chemoenzymatic Synthesis and Fluorescent Visualization of Cell-Surface Selectin-Bound Sialyl Lewis X Derivatives", CHEMISTRY, A EUROPEAN JOURNAL, vol. 6, no. 1, 2000, pages 162 - 171, XP002186413
RAND J B ET AL: "GENETIC PHARMACOLOGY: INTERACTIONS BETWEEN DRUGS AND GENE PRODUCTS IN CAENORHABDITIS ELEGANS", METHODS IN CELL BIOLOGY, LONDON, GB, vol. 84, 1995, pages 187 - 204, XP000956211
BIRD A F: "A METHOD OF DISTINGUISHING BETWEEN LIVING AND DEAD NEMATODES BY ENZYMATICALLY INDUCED FLUORESCENCE", JOURNAL OF NEMATOLOGY, vol. 11, no. 1, 1979, EN, pages 103 - 105, XP001051693, ISSN: 0022-300X
MATYASH VITALI ET AL: "Distribution and transport of cholesterol in Caenorhabditis elegans.", MOLECULAR BIOLOGY OF THE CELL, vol. 12, no. 6, June 2001 (2001-06-01), pages 1725 - 1736, XP001048139, ISSN: 1059-1524
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