Title:
LIPOXYGENASE-BASED RECOMBINANT MICROORGANISMS, AND METHOD FOR PREPARING HYDROXY FATTY ACIDS AND SECONDARY FATTY ALCOHOLS USING SAME
Document Type and Number:
WIPO Patent Application WO/2022/191638
Kind Code:
A1
Abstract:
The present invention relates to: recombinant microorganisms expressing lipoxygenase; and recombinant microorganisms co-expressing lipoxygenase and chlorella-derived decarboxylase. It was confirmed that when using lipoxygenase-based recombinant microorganisms according to the present invention as whole-cell biocatalysts, hydroxy fatty acids can be produced from unsaturated fatty acids. In addition, it was confirmed that when using the recombinant microorganisms co-expressing lipoxygenase and chlorella-derived decarboxylase as whole-cell biocatalysts, secondary fatty alcohols can be produced from unsaturated fatty acids. Thus, the recombinant microorganisms constructed in the present invention can be used in various ways in the field of hydroxy fatty acid and secondary fatty alcohol production.
Inventors:
PARK JIN BYUNG (KR)
CHA HEE JEONG (KR)
LEE JEONG HOO (KR)
YOON JEONG HYE (KR)
OH DEOK KUN (KR)
CHA HEE JEONG (KR)
LEE JEONG HOO (KR)
YOON JEONG HYE (KR)
OH DEOK KUN (KR)
Application Number:
PCT/KR2022/003365
Publication Date:
September 15, 2022
Filing Date:
March 10, 2022
Export Citation:
Assignee:
UNIV EWHA IND COLLABORATION (KR)
UNIV KONKUK IND COOP CORP (KR)
UNIV KONKUK IND COOP CORP (KR)
International Classes:
C12N1/20; C12N9/02; C12N15/70; C12P7/6409
Foreign References:
| KR20160017427A | 2016-02-16 | |||
| CN102277317A | 2011-12-14 |
Other References:
VILLAVERDE JUAN JOSÉ, SANTOS SÓNIA A.O., HAARMANN THOMAS, NETO CARLOS PASCOAL, SIMÕES MÁRIO M.Q., DOMINGUES M. ROSÁRIO M., SILVEST: "Cloned Pseudomonas aeruginosa lipoxygenase as efficient approach for the clean conversion of linoleic acid into valuable hydroperoxides", CHEMICAL ENGENEERING JOURNAL, ELSEVIER, AMSTERDAM, NL, vol. 231, 1 September 2013 (2013-09-01), AMSTERDAM, NL , pages 519 - 525, XP055964852, ISSN: 1385-8947, DOI: 10.1016/j.cej.2013.07.064
BAE JAE-HAN, HOU CHING T., KIM HAK-RYUL: "Thermostable lipoxygenase is a key enzyme in the conversion of linoleic acid to trihydroxy-octadecenoic acid by Pseudomonas aeruginosa PR3", BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, KOREAN SOCIETY FOR BIOTECHNOLOGY AND BIOENGINEERING, SEOUL, KR, vol. 15, no. 6, 1 December 2010 (2010-12-01), KR , pages 1022 - 1030, XP055964857, ISSN: 1226-8372, DOI: 10.1007/s12257-010-0273-y
VILLAVERDE JUAN JOSÉ, VAN DER VLIST VINCENT, SANTOS SÓNIA A.O., HAARMANN THOMAS, LANGFELDER KIM, PIRTTIMAA MINNI, NYYSSÖLÄ ANTTI, : "Hydroperoxide production from linoleic acid by heterologous Gaeumannomyces graminis tritici lipoxygenase: Optimization and scale-up", CHEMICAL ENGENEERING JOURNAL, ELSEVIER, AMSTERDAM, NL, vol. 217, 1 February 2013 (2013-02-01), AMSTERDAM, NL , pages 82 - 90, XP055964861, ISSN: 1385-8947, DOI: 10.1016/j.cej.2012.11.090
BAE JAE-HAN, HOU CHING T., KIM HAK-RYUL: "Thermostable lipoxygenase is a key enzyme in the conversion of linoleic acid to trihydroxy-octadecenoic acid by Pseudomonas aeruginosa PR3", BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, KOREAN SOCIETY FOR BIOTECHNOLOGY AND BIOENGINEERING, SEOUL, KR, vol. 15, no. 6, 1 December 2010 (2010-12-01), KR , pages 1022 - 1030, XP055964857, ISSN: 1226-8372, DOI: 10.1007/s12257-010-0273-y
VILLAVERDE JUAN JOSÉ, VAN DER VLIST VINCENT, SANTOS SÓNIA A.O., HAARMANN THOMAS, LANGFELDER KIM, PIRTTIMAA MINNI, NYYSSÖLÄ ANTTI, : "Hydroperoxide production from linoleic acid by heterologous Gaeumannomyces graminis tritici lipoxygenase: Optimization and scale-up", CHEMICAL ENGENEERING JOURNAL, ELSEVIER, AMSTERDAM, NL, vol. 217, 1 February 2013 (2013-02-01), AMSTERDAM, NL , pages 82 - 90, XP055964861, ISSN: 1385-8947, DOI: 10.1016/j.cej.2012.11.090
Attorney, Agent or Firm:
KIM, Soon Woong (KR)
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