LIQUID YEAST ONE- AND TWO-HYBRID HIGH-THROUGHPUT LIBRARY SCREENING METHOD AND APPLICATION THEREOF

Title:

LIQUID YEAST ONE- AND TWO-HYBRID HIGH-THROUGHPUT LIBRARY SCREENING METHOD AND APPLICATION THEREOF

Document Type and Number:

WIPO Patent Application WO/2022/193849

Kind Code:

A1

Abstract:

Provided are a liquid yeast one- and two-hybrid high-throughput library screening method and an application thereof. In the method, on the basis of a traditional yeast hybrid system, solid culture medium screening is changed into liquid culture solution screening, a large amount of plate coating work is avoided, the tedious work of picking single colonies for identification one by one is also avoided, moreover, the present invention is further combined with a high-throughput sequencing technology, so that the analysis throughput of yeast hybrid screening work is improved; moreover, the automation level of analysis can also be improved, false negative can be reduced, the method can solve the self-activation phenomenon of a protein, and known protein interaction information can be successfully obtained by using liquid screening.

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Inventors:

JIANG BINGJUN (CN)
QI YANPING (CN)
HAN TIANFU (CN)
SUN SHI (CN)
WU CUNXIANG (CN)
WU TINGTING (CN)
YUAN SHAN (CN)

Application Number:

PCT/CN2022/074316

Publication Date:

September 22, 2022

Filing Date:

January 27, 2022

Export Citation:

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Assignee:

INST OF CROP SCIENCES OF CAAS (CN)

International Classes:

C12N15/29; C12N15/04; C12N15/81; C12Q1/04; C12Q1/6869

Foreign References:

CN113046385A	2021-06-29
CN102220257A	2011-10-19
US6406863B1	2002-06-18
CN110004171A	2019-07-12
CN107034286A	2017-08-11
US20140038831A1	2014-02-06
US6187535B1	2001-02-13
CN102251025A	2011-11-23

Other References:

ZHANG KAI, LIU YING-YING;PENG DE-LIANG;WU ZHENG-DAN;MAO LI-MIN;TANG DAO-BIN;WANG JI-CHUN: "Screening and Identification of Interacting Proteins of Sweet Potato WRKY Transcription Factor IbWRKY61", JOURNAL OF AGRICULTURAL BIOTECHNOLOGY, vol. 28, no. 12, 22 October 2013 (2013-10-22), pages 2118 - 2129, XP055967016, ISSN: 1674-7968, DOI: 10.3969/j.issn.1674-7968.2020.12.003

Attorney, Agent or Firm:

JEEKAI&PARTNERS (CN)

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